CA2553485A1 - Integration multicopie et expression de genes heterologues dans un methylbacterium - Google Patents

Integration multicopie et expression de genes heterologues dans un methylbacterium Download PDF

Info

Publication number: CA2553485A1
Authority: CA; Canada
Prior art keywords: extorquens; vector; gene; promoter; methylobacterium
Prior art date: 2005-07-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

CA002553485A

Other languages

English (en)

Other versions

CA2553485C (fr

Inventor

Carlos B. Miguez

Young-Jun Choi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

National Research Council of Canada

Original Assignee

National Research Council of Canada

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2005-07-26

Filing date

2006-07-25

Publication date

2007-01-26

2006-07-25 Application filed by National Research Council of Canada filed Critical National Research Council of Canada

2007-01-26 Publication of CA2553485A1 publication Critical patent/CA2553485A1/fr

2014-02-04 Application granted granted Critical

2014-02-04 Publication of CA2553485C publication Critical patent/CA2553485C/fr

2026-07-25 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

108090000623 proteins and genes Proteins 0.000 title claims abstract description 88
241000589323 Methylobacterium Species 0.000 title claims abstract description 22
230000014509 gene expression Effects 0.000 title description 24
241000589308 Methylobacterium extorquens Species 0.000 claims abstract description 70
230000010354 integration Effects 0.000 claims abstract description 32
108010048916 alcohol dehydrogenase (acceptor) Proteins 0.000 claims abstract description 6
239000013598 vector Substances 0.000 claims description 28
102000004169 proteins and genes Human genes 0.000 claims description 20
238000004519 manufacturing process Methods 0.000 claims description 18
108090000371 Esterases Proteins 0.000 claims description 9
230000000853 biopesticidal effect Effects 0.000 claims description 3
231100000331 toxic Toxicity 0.000 claims description 3
230000002588 toxic effect Effects 0.000 claims description 3
238000012258 culturing Methods 0.000 claims description 2
229920001184 polypeptide Polymers 0.000 claims description 2
102000004196 processed proteins & peptides Human genes 0.000 claims description 2
108090000765 processed proteins & peptides Proteins 0.000 claims description 2
101150041868 cry1Aa gene Proteins 0.000 claims 1
241000894007 species Species 0.000 claims 1
101100170478 Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) moxF gene Proteins 0.000 abstract description 2
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
210000004027 cell Anatomy 0.000 description 27
239000013612 plasmid Substances 0.000 description 23
239000005090 green fluorescent protein Substances 0.000 description 17
210000000349 chromosome Anatomy 0.000 description 15
238000000855 fermentation Methods 0.000 description 14
230000004151 fermentation Effects 0.000 description 14
108020004414 DNA Proteins 0.000 description 12
238000000034 method Methods 0.000 description 12
239000012634 fragment Substances 0.000 description 11
238000003780 insertion Methods 0.000 description 11
230000037431 insertion Effects 0.000 description 11
241000894006 Bacteria Species 0.000 description 10
241000588724 Escherichia coli Species 0.000 description 10
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 9
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 9
239000002609 medium Substances 0.000 description 8
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
238000004520 electroporation Methods 0.000 description 6
239000002028 Biomass Substances 0.000 description 5
241000196324 Embryophyta Species 0.000 description 5
239000003242 anti bacterial agent Substances 0.000 description 5
229940088710 antibiotic agent Drugs 0.000 description 5
230000001580 bacterial effect Effects 0.000 description 5
238000010276 construction Methods 0.000 description 5
230000000694 effects Effects 0.000 description 5
101150117187 glmS gene Proteins 0.000 description 5
229930027917 kanamycin Natural products 0.000 description 5
229960000318 kanamycin Drugs 0.000 description 5
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
229930182823 kanamycin A Natural products 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 4
108090000790 Enzymes Proteins 0.000 description 4
241000607479 Yersinia pestis Species 0.000 description 4
239000013611 chromosomal DNA Substances 0.000 description 4
229940088598 enzyme Drugs 0.000 description 4
230000002779 inactivation Effects 0.000 description 4
239000000758 substrate Substances 0.000 description 4
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
108010043121 Green Fluorescent Proteins Proteins 0.000 description 3
108091029795 Intergenic region Proteins 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
235000008582 Pinus sylvestris Nutrition 0.000 description 3
241000218626 Pinus sylvestris Species 0.000 description 3
241000589776 Pseudomonas putida Species 0.000 description 3
238000002105 Southern blotting Methods 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
230000003115 biocidal effect Effects 0.000 description 3
229910052799 carbon Inorganic materials 0.000 description 3
238000000799 fluorescence microscopy Methods 0.000 description 3
239000007789 gas Substances 0.000 description 3
239000003550 marker Substances 0.000 description 3
239000000463 material Substances 0.000 description 3
238000005259 measurement Methods 0.000 description 3
239000012528 membrane Substances 0.000 description 3
229910052757 nitrogen Inorganic materials 0.000 description 3
238000012261 overproduction Methods 0.000 description 3
239000001301 oxygen Substances 0.000 description 3
229910052760 oxygen Inorganic materials 0.000 description 3
239000000523 sample Substances 0.000 description 3
238000012163 sequencing technique Methods 0.000 description 3
239000002689 soil Substances 0.000 description 3
230000017105 transposition Effects 0.000 description 3
229920001817 Agar Polymers 0.000 description 2
241001156002 Anthonomus pomorum Species 0.000 description 2
241000207199 Citrus Species 0.000 description 2
MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
101150066002 GFP gene Proteins 0.000 description 2
102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
108010004901 Haloalkane dehalogenase Proteins 0.000 description 2
201000008225 Klebsiella pneumonia Diseases 0.000 description 2
241000588747 Klebsiella pneumoniae Species 0.000 description 2
244000199866 Lactobacillus casei Species 0.000 description 2
235000013958 Lactobacillus casei Nutrition 0.000 description 2
239000006137 Luria-Bertani broth Substances 0.000 description 2
241000218922 Magnoliophyta Species 0.000 description 2
241000432067 Methylobacterium extorquens AM1 Species 0.000 description 2
206010035717 Pneumonia klebsiella Diseases 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
241000607715 Serratia marcescens Species 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
239000008272 agar Substances 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
238000013019 agitation Methods 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
238000012512 characterization method Methods 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
230000002759 chromosomal effect Effects 0.000 description 2
238000001514 detection method Methods 0.000 description 2
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
108010047973 enterocin P Proteins 0.000 description 2
239000013604 expression vector Substances 0.000 description 2
239000006260 foam Substances 0.000 description 2
239000013505 freshwater Substances 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
229910052500 inorganic mineral Inorganic materials 0.000 description 2
230000003993 interaction Effects 0.000 description 2
229940017800 lactobacillus casei Drugs 0.000 description 2
244000005700 microbiome Species 0.000 description 2
239000011707 mineral Substances 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
230000002018 overexpression Effects 0.000 description 2
239000002953 phosphate buffered saline Substances 0.000 description 2
238000002731 protein assay Methods 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
230000010473 stable expression Effects 0.000 description 2
230000014616 translation Effects 0.000 description 2
238000012795 verification Methods 0.000 description 2
108010011958 1,3-propanediol dehydrogenase Proteins 0.000 description 1
OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
241000186015 Bifidobacterium longum subsp. infantis Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
108010080972 Catechol 2,3-dioxygenase Proteins 0.000 description 1
241000929260 Choristoneura orae Species 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
238000007399 DNA isolation Methods 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 description 1
241000194033 Enterococcus Species 0.000 description 1
241000194031 Enterococcus faecium Species 0.000 description 1
241000282326 Felis catus Species 0.000 description 1
230000005526 G1 to G0 transition Effects 0.000 description 1
102000004894 Glutamine-fructose-6-phosphate transaminase (isomerizing) Human genes 0.000 description 1
108090001031 Glutamine-fructose-6-phosphate transaminase (isomerizing) Proteins 0.000 description 1
244000020551 Helianthus annuus Species 0.000 description 1
241000238631 Hexapoda Species 0.000 description 1
108010059881 Lactase Proteins 0.000 description 1
241000408736 Methylobacterium aquaticum Species 0.000 description 1
241000408722 Methylobacterium hispanicum Species 0.000 description 1
241000869140 Noccaea goesingensis Species 0.000 description 1
108091092724 Noncoding DNA Proteins 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
235000008124 Picea excelsa Nutrition 0.000 description 1
229920001397 Poly-beta-hydroxybutyrate Polymers 0.000 description 1
229920000331 Polyhydroxybutyrate Polymers 0.000 description 1
241000589540 Pseudomonas fluorescens Species 0.000 description 1
WHBMMWSBFZVSSR-UHFFFAOYSA-N R3HBA Natural products CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
244000269722 Thea sinensis Species 0.000 description 1
241000589494 Xanthobacter autotrophicus Species 0.000 description 1
241000204362 Xylella fastidiosa Species 0.000 description 1
238000009825 accumulation Methods 0.000 description 1
230000003044 adaptive effect Effects 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
235000011114 ammonium hydroxide Nutrition 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
230000003321 amplification Effects 0.000 description 1
238000013459 approach Methods 0.000 description 1
210000003578 bacterial chromosome Anatomy 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
229940004120 bifidobacterium infantis Drugs 0.000 description 1
238000012742 biochemical analysis Methods 0.000 description 1
239000012681 biocontrol agent Substances 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000003593 chromogenic compound Substances 0.000 description 1
238000010367 cloning Methods 0.000 description 1
238000011259 co-electroporation Methods 0.000 description 1
238000004883 computer application Methods 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
230000002950 deficient Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
101150061843 dhaT gene Proteins 0.000 description 1
238000009826 distribution Methods 0.000 description 1
238000011143 downstream manufacturing Methods 0.000 description 1
239000003651 drinking water Substances 0.000 description 1
235000020188 drinking water Nutrition 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
235000013305 food Nutrition 0.000 description 1
239000000499 gel Substances 0.000 description 1
238000003633 gene expression assay Methods 0.000 description 1
239000008187 granular material Substances 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000009396 hybridization Methods 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
208000006278 hypochromic anemia Diseases 0.000 description 1
238000007901 in situ hybridization Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
238000002372 labelling Methods 0.000 description 1
235000021374 legumes Nutrition 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
230000001450 methanotrophic effect Effects 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
238000007479 molecular analysis Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
ZHCAAFJSYLFLPX-UHFFFAOYSA-N nitrocyclohexatriene Chemical group [O-][N+](=O)C1=CC=C=C[CH]1 ZHCAAFJSYLFLPX-UHFFFAOYSA-N 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
230000005305 organ development Effects 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
230000005298 paramagnetic effect Effects 0.000 description 1
230000002572 peristaltic effect Effects 0.000 description 1
230000003032 phytopathogenic effect Effects 0.000 description 1
238000011020 pilot scale process Methods 0.000 description 1
229920001296 polysiloxane Polymers 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
239000011546 protein dye Substances 0.000 description 1
238000000751 protein extraction Methods 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000003259 recombinant expression Methods 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
239000013049 sediment Substances 0.000 description 1
239000004065 semiconductor Substances 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
239000000243 solution Substances 0.000 description 1
238000002798 spectrophotometry method Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
230000009897 systematic effect Effects 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1

Classifications

- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

CA2553485A 2005-07-26 2006-07-25 Integration multicopie et expression de genes heterologues dans un methylbacterium Expired - Fee Related CA2553485C (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US70229105P	2005-07-26	2005-07-26
US60/702,291		2005-07-26

Publications (2)

Publication Number	Publication Date
CA2553485A1 true CA2553485A1 (fr)	2007-01-26
CA2553485C CA2553485C (fr)	2014-02-04

Family

ID=37682464

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2553485A Expired - Fee Related CA2553485C (fr)	2005-07-26	2006-07-25	Integration multicopie et expression de genes heterologues dans un methylbacterium

Country Status (1)

Country	Link
CA (1)	CA2553485C (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8216821B2 (en)	2005-08-23	2012-07-10	National Research Council Of Canada	Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria

2006
- 2006-07-25 CA CA2553485A patent/CA2553485C/fr not_active Expired - Fee Related

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US8216821B2 (en)	2005-08-23	2012-07-10	National Research Council Of Canada	Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria

Also Published As

Publication number	Publication date
CA2553485C (fr)	2014-02-04

Legal Events

Date	Code	Title	Description
2011-01-12	EEER	Examination request
2022-04-12	MKLA	Lapsed	Effective date: 20220301
2022-10-06	MKLA	Lapsed	Effective date: 20200831

Publication	Publication Date	Title
Vary et al.	2007	Bacillus megaterium—from simple soil bacterium to industrial protein production host
US7670824B2 (en)	2010-03-02	Multicopy-integration of heterologous genes and expression in Methylobacterium
Vary	1994	Prime time for Bacillus megaterium
Tutino et al.	2001	A novel replication element from an Antarctic plasmid as a tool for the expression of proteins at low temperature
CA2372556A1 (fr)	2000-11-23	Evolution dirigee de micro-organismes
US8216821B2 (en)	2012-07-10	Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
Feng et al.	2014	Metabolic engineering of B acillus amyloliquefaciens for poly‐gamma‐glutamic acid (γ‐PGA) overproduction
Choi et al.	2006	Multicopy integration and expression of heterologous genes in Methylobacterium extorquens ATCC 55366
Murakami et al.	2003	Cloning and functional analysis of aniline dioxygenase gene cluster, from Frateuria species ANA-18, that metabolizes aniline via an ortho-cleavage pathway of catechol
US12378587B2 (en)	2025-08-05	Recombinant methanotrophic bacteria for indigo biosynthesis and methods thereof
Srinivasan et al.	2003	Production of recombinant proteins using multiple‐copy gene integration in high‐cell‐density fermentations of Ralstonia eutropha
WO2022069644A1 (fr)	2022-04-07	Bactéries methylobacillus génétiquement modifiées ayant des propriétés améliorées
WO2022018260A1 (fr)	2022-01-27	Délétion unique et transcomplémentation de l'alanine racémase
Schweder et al.	1992	An expression vector system providing plasmid stability and conditional suicide of plasmid-containing cells
Liang et al.	2007	Expression of Vitreoscilla hemoglobin in Bacillus thuringiensis improve the cell density and insecticidal crystal proteins yield
CN113461789B (zh)	2022-05-27	一种来源于伯克霍尔德氏菌的LysR家族转录调控蛋白、基因及应用
CA2553485C (fr)	2014-02-04	Integration multicopie et expression de genes heterologues dans un methylbacterium
US20060172423A1 (en)	2006-08-03	New expression system from rhodococcus
CN114630895A (zh)	2022-06-14	用于增加地衣芽孢杆菌中蛋白质生产的组合物和方法
US20160115490A1 (en)	2016-04-28	Bacterial Mutants with Improved Transformation Efficiency
WO1994023042A1 (fr)	1994-10-13	Procede pour augmenter la production de proteines de recombinaison actives sur le plan biologique
CN116897160A (zh)	2023-10-17	在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物
CA1304705C (fr)	1992-07-07	Systemes organiques
Ji et al.	2015	Enhancement of gellan production in Sphingomonas paucimobilis JLJ by heterogeneous expression of Vitreoscilla hemoglobin
MATSUNO et al.	1992	Transformation of Bacillus subtilis, antifungal-antibiotic iturin producers with isolated antibiotic resistance plasmids

CA2553485A1 - Integration multicopie et expression de genes heterologues dans un methylbacterium - Google Patents

Info

Links

Classifications

Landscapes

Applications Claiming Priority (2)

Publications (2)

Family

ID=37682464

Family Applications (1)

Country Status (1)

Cited By (1)

Cited By (1)

Also Published As

Similar Documents

Legal Events