CA2573972C - Procede permettant d'augmenter la teneur d'organismes transgeniques en acides gras a chaine longue polyinsatures - Google Patents

Procede permettant d'augmenter la teneur d'organismes transgeniques en acides gras a chaine longue polyinsatures Download PDF

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Publication number
CA2573972C
CA2573972C CA2573972A CA2573972A CA2573972C CA 2573972 C CA2573972 C CA 2573972C CA 2573972 A CA2573972 A CA 2573972A CA 2573972 A CA2573972 A CA 2573972A CA 2573972 C CA2573972 C CA 2573972C
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Canada
Prior art keywords
seq
nucleic acid
acid
desaturase
fatty acids
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Expired - Fee Related
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CA2573972A
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English (en)
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CA2573972A1 (fr
Inventor
Petra Cirpus
Joerg Bauer
Xiao Qiu
Patricia Vrinten
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BASF Plant Science GmbH
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BASF Plant Science GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Seasonings (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Edible Oils And Fats (AREA)

Abstract

L'invention concerne un procédé permettant d'augmenter la teneur d'un organisme en acides gras à chaîne longue polyinsaturés. Selon ce procédé, on introduit dans l'organisme des acides nucléiques qui sont codants pour les polypeptides ou protéines ayant une activité de phospholipase, cétoacyl-CoA-réductase et/ou déshydratase. De préférence, les enzymes sont issus d'ostreococcus ou de thraustochytrium. L'invention concerne également les séquences d'acides nucléiques, les structures d'acides nucléiques, les vecteurs et organismes contenant les séquences d'acides nucléiques selon l'invention, les vecteurs contenant les séquences d'acides nucléiques et/ou les structures d'acides nucléiques ainsi que les organismes transgéniques contenant les séquences d'acides nucléiques, les structures d'acides nucléiques et/ou les vecteurs précités. De préférence, les séquences d'acides nucléiques, les structures d'acides nucléiques et les vecteurs précités peuvent, éventuellement avec d'autres séquences d'acides nucléiques qui sont codantes pour des polypeptides ou protéines de la biosynthèse du métabolisme des acides gras ou des lipides, être exprimés dans l'organisme. Des séquences d'acides nucléiques particulièrement avantageuses du métabolisme des acides gras ou des lipides sont codantes pour une activité de .DELTA.-9 élongase, .DELTA.-8-désaturase, .DELTA.-5-désaturase, .DELTA.-4-désaturase, .DELTA.-12-désaturase, .DELTA.-5-élongase et/ou .DELTA.-6-élongase. De préférence, ces désaturases et élongases proviennent des organismes suivants Thalassiosira, Euglena, Isochrysis, Physcomitrella, Thraustochytrium, Borago, Phytophthora, Crypthecodinium, Oncorhynchus, Primula, Xenopus, Ciona, Arabidopsis, Mortierella, Caenorhabditis, Phaeodactylum, Ceratodon ou Ostreococcus. Un autre aspect de l'invention concerne les huiles, lipides et/ou les acides gras produits d'après ce procédé et leur utilisation. L'invention concerne enfin des acides gras insaturés et des triglycérides ayant une teneur accrue en acides gras insaturés et leur utilisation.
CA2573972A 2004-07-16 2005-07-15 Procede permettant d'augmenter la teneur d'organismes transgeniques en acides gras a chaine longue polyinsatures Expired - Fee Related CA2573972C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102004034442 2004-07-16
DE102004034442.6 2004-07-16
DE102004060340A DE102004060340A1 (de) 2004-07-16 2004-12-15 Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
DE102004060340.5 2004-12-15
PCT/EP2005/007754 WO2006008099A2 (fr) 2004-07-16 2005-07-15 Procede permettant d'augmenter la teneur d'organismes transgeniques en acides gras a chaine longue polyinsatures

Publications (2)

Publication Number Publication Date
CA2573972A1 CA2573972A1 (fr) 2006-01-26
CA2573972C true CA2573972C (fr) 2016-06-07

Family

ID=35612959

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2573972A Expired - Fee Related CA2573972C (fr) 2004-07-16 2005-07-15 Procede permettant d'augmenter la teneur d'organismes transgeniques en acides gras a chaine longue polyinsatures

Country Status (6)

Country Link
US (1) US7842852B2 (fr)
EP (1) EP1769074B1 (fr)
AT (1) ATE445703T1 (fr)
CA (1) CA2573972C (fr)
DE (2) DE102004060340A1 (fr)
WO (1) WO2006008099A2 (fr)

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US7211418B2 (en) 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
ES2713548T3 (es) 2003-08-01 2019-05-22 Basf Plant Science Gmbh Procedimiento para la producción de ácidos grasos poliinsaturados en organismos transgénicos
EP2623584B1 (fr) 2004-02-27 2019-04-10 BASF Plant Science GmbH Procédé de fabrication de plusieurs acides gras insaturés dans des plantes transgéniques
WO2005083053A2 (fr) 2004-02-27 2005-09-09 Basf Plant Science Gmbh Procede pour produire des acides gras $g(v)-3 insatures dans des organismes transgeniques
DK1756280T3 (en) 2004-04-22 2015-02-02 Commw Scient Ind Res Org SYNTHESIS OF CHAIN, polyunsaturated fatty acids BY RECOMBINANT CELLS
CA3056110C (fr) 2004-04-22 2020-07-14 Surinder Pal Singh Synthese d'acides gras polyinsatures a chaine longue par des cellules de recombinaison
AR059376A1 (es) 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
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US9090902B2 (en) * 2008-08-26 2015-07-28 Basf Plant Science Gmbh Nucleic acids encoding desaturases and modified plant oil
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WO2011090493A1 (fr) 2010-01-19 2011-07-28 Martek Biosciences Corporation Micro-organismes produisant de l'acide eicosapentaénoïque, compositions d'acides gras et leurs procédés de fabrication et d'utilisation
CA2808002A1 (fr) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Delta-5 desaturases a motif hpgg et a motif hdash mutes et leur utilisation dans la fabrication d'acides gras polyinsatures
CN103703135B (zh) * 2011-05-27 2015-12-02 淡马锡生命科学研究院有限公司 微粒体ω6油酸去饱和酶
HK1198515A1 (en) 2011-07-21 2015-05-15 Dsm Ip Assets B.V. Fatty acid compositions
BR112014025265A2 (pt) * 2012-04-12 2017-07-11 Rothamsted Res Ltd produção de ácidos graxos poli-insaturados ômega-3 de cadeia longa
PL2861059T3 (pl) 2012-06-15 2017-10-31 Commw Scient Ind Res Org Wytwarzanie długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach roślinnych
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CA2510462A1 (fr) 2002-12-19 2004-07-08 University Of Bristol Methode de production d'acides gras polyinsatures dans une plante transgenique ou un micro-organisme comprenant une delta-9-elongase et une delta-8-desaturase introduites

Also Published As

Publication number Publication date
DE502005008336D1 (de) 2009-11-26
US20080076164A1 (en) 2008-03-27
EP1769074A2 (fr) 2007-04-04
DE102004060340A1 (de) 2006-02-09
WO2006008099A2 (fr) 2006-01-26
CA2573972A1 (fr) 2006-01-26
ATE445703T1 (de) 2009-10-15
EP1769074B1 (fr) 2009-10-14
WO2006008099A3 (fr) 2007-03-15
US7842852B2 (en) 2010-11-30

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