CA2687062C - Procedes et systemes d'analyse de matiere fluorescente a autofluorescence limitee - Google Patents

Procedes et systemes d'analyse de matiere fluorescente a autofluorescence limitee Download PDF

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Publication number
CA2687062C
CA2687062C CA2687062A CA2687062A CA2687062C CA 2687062 C CA2687062 C CA 2687062C CA 2687062 A CA2687062 A CA 2687062A CA 2687062 A CA2687062 A CA 2687062A CA 2687062 C CA2687062 C CA 2687062C
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CA
Canada
Prior art keywords
illumination
systems
fluorescent
substrate
optical
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Active
Application number
CA2687062A
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English (en)
Other versions
CA2687062A1 (fr
Inventor
Paul Lundquist
Cheng-Frank Zhong
Denis Zaccarin
Peiqian Zhao
Stephen Turner
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Pacific Biosciences of California Inc
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Pacific Biosciences of California Inc
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Publication date
Priority claimed from US11/901,273 external-priority patent/US20080277595A1/en
Application filed by Pacific Biosciences of California Inc filed Critical Pacific Biosciences of California Inc
Publication of CA2687062A1 publication Critical patent/CA2687062A1/fr
Application granted granted Critical
Publication of CA2687062C publication Critical patent/CA2687062C/fr
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Des approches réductrices et correctives permettant de limiter le bruit parasite d'autofluorescence sont appliquées dans les systèmes analytiques reposant sur la mesure sensible de signaux fluorescents émis par des réseaux de sources de signaux fluorescents. Ces systèmes sont particulièrement utiles dans le séquençage basé sur la fluorescence par des systèmes d'intégration reposant sur des petits effectifs ou des molécules fluorescentes individuelles qui détectent l'intégration de nucléotides dans des réactions d'extension d'amorces. L'invention concerne des systèmes et des procédés permettant d'analyser des réseaux d'échantillons extrêmement multiplexés en utilisant des systèmes optiques de haute densité, fortement multiplexés, pour éclairer les réseaux d'échantillons de haute densité et/ou obtenir une détection et de préférence une détection confocale sans signaux émanant de ces réseaux de haute densité. Les systèmes et les procédés sont appliqués selon diverses opérations analytiques différentes, y compris l'analyse de réactions biologiques et biochimiques, y compris la synthèse d'acides nucléiques et la dérivation d'informations de séquences provenant de cette synthèse.
CA2687062A 2007-05-10 2008-05-09 Procedes et systemes d'analyse de matiere fluorescente a autofluorescence limitee Active CA2687062C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US92861707P 2007-05-10 2007-05-10
US60/928,617 2007-05-10
US11/901,273 US20080277595A1 (en) 2007-05-10 2007-09-14 Highly multiplexed confocal detection systems and methods of using same
US11/901,273 2007-09-14
PCT/US2008/005953 WO2008140758A1 (fr) 2007-05-10 2008-05-09 Procédés et systèmes d'analyse de matière fluorescente à autofluorescence limitée

Publications (2)

Publication Number Publication Date
CA2687062A1 CA2687062A1 (fr) 2008-11-20
CA2687062C true CA2687062C (fr) 2016-04-12

Family

ID=41382245

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2687062A Active CA2687062C (fr) 2007-05-10 2008-05-09 Procedes et systemes d'analyse de matiere fluorescente a autofluorescence limitee

Country Status (4)

Country Link
EP (1) EP2150806A4 (fr)
AU (1) AU2008251861B2 (fr)
CA (1) CA2687062C (fr)
WO (1) WO2008140758A1 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
EP2881736B1 (fr) 2008-03-31 2017-06-07 Pacific Biosciences of California, Inc. Procédés de chargement de polymerase unique et compositions
EP2362947B1 (fr) 2008-09-30 2017-05-10 Pacific Biosciences of California, Inc. Systèmes analytiques à multiplexage ultra-élevé et procédés
US8906670B2 (en) 2009-09-11 2014-12-09 Pacific Bioscience Of California, Inc. Zero-mode waveguides with non-reflecting walls
US8367159B2 (en) 2009-09-11 2013-02-05 Pacific Biosciences Of California, Inc. Methods for producing ZMWs with islands of functionality
EP2491370B1 (fr) 2009-10-23 2023-03-01 Koninklijke Philips N.V. Dispositif pour surveiller une pluralité de signaux discrets de fluorescence
EP2564191B1 (fr) 2010-04-28 2017-11-01 Pacific Biosciences Of California, Inc. Ouvertures à l'échelle du nanomètre possédant des îlots de fonctionnalité
JP2013525811A (ja) * 2010-05-03 2013-06-20 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア オンチップの広視野のレンズフリー蛍光画像化
US9581550B2 (en) * 2013-05-31 2017-02-28 Pacific Biosciences Of California Analytical devices having compact lens train arrays
WO2015036732A1 (fr) 2013-09-10 2015-03-19 Bae Systems Plc Mesure d'une rugosité de surface optique
KR102239487B1 (ko) 2018-01-08 2021-04-14 일루미나, 인코포레이티드 반도체-기반 검출을 사용한 고-처리율 서열분석
NL2020621B1 (en) * 2018-01-08 2019-07-15 Illumina Inc Multiplexing of an active sensor detector using structured illumination
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
CN108181290B (zh) * 2018-03-24 2020-07-10 王碧 一种荧光定量分析仪
WO2021081129A1 (fr) 2019-10-21 2021-04-29 Illumina, Inc. Efficacité de calcul accrue pour la microscopie à éclairage structuré
CN117330542A (zh) * 2022-06-24 2024-01-02 华中科技大学苏州脑空间信息研究院 高对比度各向同性高分辨荧光显微成像方法及成像系统
EP4379355A1 (fr) * 2022-11-29 2024-06-05 Gnothis Holding AG Analyse à haut débit d'événements de molécule unique

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6055106A (en) * 1998-02-03 2000-04-25 Arch Development Corporation Apparatus for applying optical gradient forces
WO2001002850A1 (fr) * 1999-07-06 2001-01-11 Caliper Technologies Corp. Systemes et methodes microfluidiques permettant de determiner la cinetique d'un modulateur
DE10126083A1 (de) * 2001-05-29 2002-12-05 Gnothis Holding Sa Ecublens Verwendung von optischen Diffraktionselementen in Nachweisverfahren
WO2003001178A2 (fr) * 2001-06-20 2003-01-03 Arryx, Inc. Jeu ordonne tridimensionnel dynamique de sondes
US20030031596A1 (en) * 2001-08-09 2003-02-13 Yokogawa Electric Corporation Biochip reader and fluorometric imaging apparatus
DE102004034951A1 (de) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Verfahren zur bildlichen Erfassung von Objekten mittels eines Lichtrastermikrokopes mit linienförmiger Abtastung
CA2579150C (fr) * 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Appareil et procede d'analyse de molecules
DE102005009188A1 (de) * 2005-03-01 2006-09-07 Carl Zeiss Jena Gmbh Punktscannendes Laser-Scanning-Mikroskop sowie Verfahren zur Einstellung eines Mikroskopes
WO2006127967A2 (fr) * 2005-05-25 2006-11-30 Massachusetts Institute Of Technology Systemes d'imagerie multifocaux et procedes associes

Also Published As

Publication number Publication date
EP2150806A4 (fr) 2013-01-02
EP2150806A1 (fr) 2010-02-10
WO2008140758A1 (fr) 2008-11-20
AU2008251861B2 (en) 2014-03-20
AU2008251861A1 (en) 2008-11-20
CA2687062A1 (fr) 2008-11-20

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