CA2723536C - Preparation d'echantillon d'acide nucleique base sur un ecoulement lateral hautement simplifie et regulation d'ecoulement de fluide passive - Google Patents
Preparation d'echantillon d'acide nucleique base sur un ecoulement lateral hautement simplifie et regulation d'ecoulement de fluide passive Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
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Abstract
Linvention propose des procédés de préparation déchantillon dacide nucléique chromatographique à écoulement latéral hautement simplifiés, des dispositifs et systèmes intégrés pour la concentration efficace déchantillons à létat de traces et lélimination dinhibiteurs damplification dacide nucléique. Linvention propose également des procédés pour capturer et réduire des inhibiteurs de réactions damplification dacide nucléique, tels que lacide humique, au moyen déléments traités par polyvinylpyrrolidone du dispositif découlement latéral, ainsi que des procédés de régulation de fluide passive et des systèmes à utiliser dans des essais à écoulement latéral.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12664508P | 2008-05-05 | 2008-05-05 | |
| US61/126,645 | 2008-05-05 | ||
| PCT/US2009/002809 WO2009137059A1 (fr) | 2008-05-05 | 2009-05-05 | Préparation d’échantillon d’acide nucléique basé sur un écoulement latéral hautement simplifié et régulation d’écoulement de fluide passive |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2723536A1 CA2723536A1 (fr) | 2009-11-12 |
| CA2723536C true CA2723536C (fr) | 2017-06-13 |
Family
ID=41264882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2723536A Active CA2723536C (fr) | 2008-05-05 | 2009-05-05 | Preparation d'echantillon d'acide nucleique base sur un ecoulement lateral hautement simplifie et regulation d'ecoulement de fluide passive |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9207236B2 (fr) |
| EP (2) | EP3067694A1 (fr) |
| JP (2) | JP2011522521A (fr) |
| CN (1) | CN102084238B (fr) |
| CA (1) | CA2723536C (fr) |
| WO (1) | WO2009137059A1 (fr) |
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2009
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- 2009-05-05 JP JP2011508502A patent/JP2011522521A/ja active Pending
- 2009-05-05 EP EP15196744.5A patent/EP3067694A1/fr not_active Ceased
- 2009-05-05 CN CN200980125614.XA patent/CN102084238B/zh active Active
- 2009-05-05 EP EP09743046.6A patent/EP2279403B1/fr active Active
- 2009-05-05 WO PCT/US2009/002809 patent/WO2009137059A1/fr not_active Ceased
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|---|---|
| US9207236B2 (en) | 2015-12-08 |
| HK1158312A1 (zh) | 2012-07-13 |
| EP3067694A1 (fr) | 2016-09-14 |
| CN102084238B (zh) | 2016-06-01 |
| EP2279403A4 (fr) | 2011-11-23 |
| JP2015108637A (ja) | 2015-06-11 |
| US20160083716A1 (en) | 2016-03-24 |
| US9944922B2 (en) | 2018-04-17 |
| CN102084238A (zh) | 2011-06-01 |
| CA2723536A1 (fr) | 2009-11-12 |
| EP2279403A1 (fr) | 2011-02-02 |
| EP2279403B1 (fr) | 2016-03-16 |
| JP6026570B2 (ja) | 2016-11-16 |
| US20110117540A1 (en) | 2011-05-19 |
| WO2009137059A1 (fr) | 2009-11-12 |
| JP2011522521A (ja) | 2011-08-04 |
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