CA2741119C - Procedes et compositions pour inactiver l'expression du gene de la glutamine synthetase - Google Patents

Procedes et compositions pour inactiver l'expression du gene de la glutamine synthetase Download PDF

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Publication number
CA2741119C
CA2741119C CA2741119A CA2741119A CA2741119C CA 2741119 C CA2741119 C CA 2741119C CA 2741119 A CA2741119 A CA 2741119A CA 2741119 A CA2741119 A CA 2741119A CA 2741119 C CA2741119 C CA 2741119C
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cell
seq
gene
zinc finger
cells
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CA2741119A1 (fr
Inventor
Pei-Qi Liu
Jeffrey C. Miller
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Sangamo Therapeutics Inc
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Sangamo Therapeutics Inc
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Publication of CA2741119A1 publication Critical patent/CA2741119A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention porte sur des procédés et des compositions pour inactiver un gène de la glutamine synthétase (GS), à l'aide de protéines de fusion comprenant une protéine en doigt de zinc et un domaine de clivage ou un demi-domaine de clivage. L'invention porte également sur des polynucléotides codant pour lesdites protéines de fusion, ainsi que sur des cellules comprenant lesdits polynucléotides et lesdites protéines de fusion.
CA2741119A 2008-10-29 2009-10-29 Procedes et compositions pour inactiver l'expression du gene de la glutamine synthetase Active CA2741119C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19760008P 2008-10-29 2008-10-29
US61/197,600 2008-10-29
PCT/US2009/005865 WO2010053518A2 (fr) 2008-10-29 2009-10-29 Procédés et compositions pour inactiver l'expression du gène de la glutamine synthétase

Publications (2)

Publication Number Publication Date
CA2741119A1 CA2741119A1 (fr) 2010-05-14
CA2741119C true CA2741119C (fr) 2019-02-12

Family

ID=42153446

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2741119A Active CA2741119C (fr) 2008-10-29 2009-10-29 Procedes et compositions pour inactiver l'expression du gene de la glutamine synthetase

Country Status (9)

Country Link
US (4) US8153399B2 (fr)
EP (1) EP2344660B1 (fr)
JP (2) JP5756016B2 (fr)
KR (1) KR101673566B1 (fr)
CN (1) CN102264915B (fr)
AU (1) AU2009311697B2 (fr)
CA (1) CA2741119C (fr)
IL (2) IL212454A (fr)
WO (1) WO2010053518A2 (fr)

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JP5756016B2 (ja) 2008-10-29 2015-07-29 サンガモ バイオサイエンシーズ， インコーポレイテッド グルタミンシンセターゼ遺伝子の発現を不活性化する方法及び組成物

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US20130164785A1 (en) 2013-06-27
US20150087067A1 (en) 2015-03-26
US20100129869A1 (en) 2010-05-27
JP2015147820A (ja) 2015-08-20
IL238983A0 (en) 2015-07-30
KR20110075021A (ko) 2011-07-05
US20100311124A1 (en) 2010-12-09
IL212454A0 (en) 2011-06-30
US8153399B2 (en) 2012-04-10
AU2009311697A1 (en) 2010-05-14
EP2344660B1 (fr) 2018-01-17
CN102264915B (zh) 2014-04-16
CA2741119A1 (fr) 2010-05-14
EP2344660A2 (fr) 2011-07-20
KR101673566B1 (ko) 2016-11-07
WO2010053518A3 (fr) 2010-07-22
AU2009311697B2 (en) 2014-12-18
WO2010053518A2 (fr) 2010-05-14
IL212454A (en) 2015-11-30
JP5756016B2 (ja) 2015-07-29
EP2344660A4 (fr) 2013-05-29
JP2012507282A (ja) 2012-03-29
US9388426B2 (en) 2016-07-12

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