CA2760941C - Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes - Google Patents

Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes Download PDF

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Publication number
CA2760941C
CA2760941C CA2760941A CA2760941A CA2760941C CA 2760941 C CA2760941 C CA 2760941C CA 2760941 A CA2760941 A CA 2760941A CA 2760941 A CA2760941 A CA 2760941A CA 2760941 C CA2760941 C CA 2760941C
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electrode
impedance
cells
cell
recording
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CA2760941A1 (fr
Inventor
Xiaobo Wang
Wei Ouyang
Nan Li
Biao Xi
Yama A. Abassi
Xiao Xu
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Agilent Technologies Inc
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Acea Biosciences Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des dispositifs et des procédés pour effectuer un enregistrement extracellulaire de cellules, telles que des cellules pouvant être excitées, des cardiomyocytes, et des cellules précurseurs de cardiomyocytes. Un dispositif cité en exemple comprend un substrat non conducteur formant une base ou fourni comme base d'un ou plusieurs puits ; une électrode d'enregistrement positionnée sur le substrat à l'intérieur du puits, l'électrode d'enregistrement étant accessible aux cellules lorsqu'un échantillon de cellules est ajouté au dispositif ; et une électrode de référence positionnée à l'intérieur du puits dans une zone exempte de cellules, la zone exempte de cellules étant caractérisée en ce qu'elle n'est pas en contact avec les cellules lorsque l'échantillon de cellules est ajouté au dispositif, empêchant ainsi tout contact entre les cellules et l'électrode de référence.
CA2760941A 2009-05-05 2010-05-05 Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes Active CA2760941C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US17556609P 2009-05-05 2009-05-05
US61/175,566 2009-05-05
US31055710P 2010-03-04 2010-03-04
US61/310,557 2010-03-04
US32378210P 2010-04-13 2010-04-13
US61/323,782 2010-04-13
PCT/US2010/033801 WO2010129725A1 (fr) 2009-05-05 2010-05-05 Système et procédé destinés à suivre le battement, la viabilité et la morphologie des cardiomyocytes et à cribler des molécules à la recherche d'agents pharmacologiques qui peuvent induire une cardiotoxicité ou moduler la fonction des cardiomyocytes

Publications (2)

Publication Number Publication Date
CA2760941A1 CA2760941A1 (fr) 2010-11-11
CA2760941C true CA2760941C (fr) 2019-07-23

Family

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CA2760941A Active CA2760941C (fr) 2009-05-05 2010-05-05 Systeme et procede destines a suivre le battement, la viabilite et la morphologie des cardiomyocytes et a cribler des molecules a la recherche d'agents pharmacologiques qui peuven t induire une cardiotoxicite ou moduler la fonction des cardiomyocytes

Country Status (3)

Country Link
EP (1) EP2427543A4 (fr)
CA (1) CA2760941C (fr)
WO (1) WO2010129725A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192752B2 (en) 2002-12-20 2007-03-20 Acea Biosciences Real time electronic cell sensing systems and applications for cell-based assays
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
WO2009137440A1 (fr) 2008-05-05 2009-11-12 Acea Biosciences, Inc. Surveillance sans marqueur d’un couplage excitation-contraction et cellules pouvant être excitées utilisant des systèmes fondés sur l'impédance avec une résolution dans le temps de l'ordre de la milliseconde
WO2011146531A1 (fr) 2010-05-18 2011-11-24 Acea Biosciences, Inc Analyse de données de signaux de battements de cardiomyocytes à base d'impédance tels que détectés sur des instruments cardiologiques d'analyse de cellules en temps réel (rtca)
WO2012178095A1 (fr) * 2011-06-22 2012-12-27 Ifluidics, Inc. Système électromoteur pour le criblage à haut débit
WO2014085933A1 (fr) 2012-12-07 2014-06-12 The Governing Council Of The University Of Toronto Constructions de tissu cardiaque et leurs procédés de fabrication
US12066428B2 (en) 2015-11-20 2024-08-20 Agilent Technologies, Inc. Cell-substrate impedance monitoring of cancer cells
EP3589299B1 (fr) 2017-03-03 2025-04-09 Agilent Technologies, Inc. Procédés et systèmes pour la maturation fonctionnelle de cardiomyocytes dérivés de cspi et de cellule se
EP3668966B1 (fr) 2017-08-16 2022-09-28 Amgen Inc. Arrangement d'electrodes adaptatif pour la mise en oueuvre dans un système incubateur de cellules et ses utilisations
US20210301245A1 (en) 2020-03-29 2021-09-30 Agilent Technologies, Inc. Systems and methods for electronically and optically monitoring biological samples
KR102629870B1 (ko) * 2021-05-14 2024-01-29 (주) 넥셀 인간 줄기세포 유래 심근세포를 이용한 약물의 심장 안전성 평가 방법
CN113655102A (zh) * 2021-08-25 2021-11-16 深圳市溢鑫科技研发有限公司 一种电化学测量微电极结构及其制作方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981268A (en) * 1997-05-30 1999-11-09 Board Of Trustees, Leland Stanford, Jr. University Hybrid biosensors
JPH11187865A (ja) * 1997-12-25 1999-07-13 Matsushita Electric Ind Co Ltd 細胞電位測定電極及びこれを用いた測定装置
US8206903B2 (en) * 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
EP1527328B1 (fr) * 2002-07-20 2009-09-09 Acea Biosciences, Inc. Appareils et procedes a base d'impedance pour l'analyse de cellules et de particules
GB0414546D0 (en) * 2004-06-29 2004-08-04 Oxford Biosensors Ltd Electrode for electrochemical sensor
US8702922B2 (en) * 2007-09-18 2014-04-22 Reprocell, Inc. Cell measuring vessel, extracellular potential measuring method, and chemical testing method

Also Published As

Publication number Publication date
EP2427543A1 (fr) 2012-03-14
WO2010129725A1 (fr) 2010-11-11
EP2427543A4 (fr) 2017-11-29
CA2760941A1 (fr) 2010-11-11

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