CA2820739A1 - Technique de transformation de microspores par macro-injection - Google Patents

Technique de transformation de microspores par macro-injection Download PDF

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Publication number
CA2820739A1
CA2820739A1 CA2820739A CA2820739A CA2820739A1 CA 2820739 A1 CA2820739 A1 CA 2820739A1 CA 2820739 A CA2820739 A CA 2820739A CA 2820739 A CA2820739 A CA 2820739A CA 2820739 A1 CA2820739 A1 CA 2820739A1
Authority
CA
Canada
Prior art keywords
plant
nanocarrier
microspores
macroinjection
buds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2820739A
Other languages
English (en)
Inventor
Pankaj K. Bhowmik
Joan Dirpaul
Alison M. R. Ferrie
Patricia L. Polowick
Hicham Fenniri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Publication of CA2820739A1 publication Critical patent/CA2820739A1/fr
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CA2820739A 2010-12-08 2011-12-07 Technique de transformation de microspores par macro-injection Abandoned CA2820739A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42090010P 2010-12-08 2010-12-08
US61/420,900 2010-12-08
PCT/CA2011/001326 WO2012075562A1 (fr) 2010-12-08 2011-12-07 Technique de transformation de microspores par macro-injection

Publications (1)

Publication Number Publication Date
CA2820739A1 true CA2820739A1 (fr) 2012-06-14

Family

ID=46206485

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2820739A Abandoned CA2820739A1 (fr) 2010-12-08 2011-12-07 Technique de transformation de microspores par macro-injection

Country Status (2)

Country Link
CA (1) CA2820739A1 (fr)
WO (1) WO2012075562A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103314845A (zh) * 2013-02-21 2013-09-25 江苏省中国科学院植物研究所 一种用单壁碳纳米管缩短彩色马蹄莲组培繁殖周期的方法
CN106342689B (zh) * 2016-09-06 2018-09-28 海南大学 一种南美星油藤快速繁殖的方法
US11371056B2 (en) 2017-03-07 2022-06-28 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
US11180770B2 (en) 2017-03-07 2021-11-23 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
CN115843796B (zh) * 2023-02-27 2023-05-12 四川科宏达集团有限责任公司 一种含有双子表面活性剂的除草剂组合物

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2485180C2 (ru) * 2007-06-07 2013-06-20 Эгрикалча Энд Эгри-Фуд Кэнэда Способ трансфекции и трансдукции растительных клеток

Also Published As

Publication number Publication date
WO2012075562A1 (fr) 2012-06-14

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20171207