CA2865254C - Compositions et procedes pour tests de diagnostic in vitro comportant de l'acide sulfonique - Google Patents

Compositions et procedes pour tests de diagnostic in vitro comportant de l'acide sulfonique Download PDF

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Publication number
CA2865254C
CA2865254C CA2865254A CA2865254A CA2865254C CA 2865254 C CA2865254 C CA 2865254C CA 2865254 A CA2865254 A CA 2865254A CA 2865254 A CA2865254 A CA 2865254A CA 2865254 C CA2865254 C CA 2865254C
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Prior art keywords
acid
composition
analyte
sulfonic acid
peroxidase
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CA2865254A1 (fr
Inventor
Gary Opperman
Wendy Nelson
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Surmodics IVD Inc
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Surmodics IVD Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des compositions, des trousses et des procédés pour effectuer une analyse colorimétrique. Un substrat est soumis à une réaction pour générer un produit de réaction chromogène, et un réactif d'interruption de réaction qui est un acide sulfonique est additionné pour interrompre et stabiliser le produit de réaction. Les propriétés d'absorbance du produit de réaction chromogène peuvent être maintenues sur des périodes de temps sensiblement plus longues que celles des réactifs et procédés classiques. L'acide sulfonique peut être utilisé dans des méthodes de dosage telles que les méthodes ELISA pour fournir une détection plus précise et plus sure d'analytes dans un échantillon biologique.
CA2865254A 2012-03-23 2013-03-22 Compositions et procedes pour tests de diagnostic in vitro comportant de l'acide sulfonique Active CA2865254C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261614602P 2012-03-23 2012-03-23
US61/614,602 2012-03-23
PCT/US2013/033429 WO2013142758A1 (fr) 2012-03-23 2013-03-22 Compositions et procédés pour tests de diagnostic in vitro comportant de l'acide sulfonique

Publications (2)

Publication Number Publication Date
CA2865254A1 CA2865254A1 (fr) 2013-09-26
CA2865254C true CA2865254C (fr) 2020-10-13

Family

ID=48143356

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2865254A Active CA2865254C (fr) 2012-03-23 2013-03-22 Compositions et procedes pour tests de diagnostic in vitro comportant de l'acide sulfonique

Country Status (5)

Country Link
US (2) US9229005B2 (fr)
EP (1) EP2841592B8 (fr)
JP (1) JP6437430B2 (fr)
CA (1) CA2865254C (fr)
WO (1) WO2013142758A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2865254C (fr) * 2012-03-23 2020-10-13 Surmodics, Inc. Compositions et procedes pour tests de diagnostic in vitro comportant de l'acide sulfonique
CN103884833B (zh) * 2014-02-17 2015-07-08 江苏硕世生物科技有限公司 一种用于增加显色持续时间的elisa酶免显色剂及其制备方法和应用
JP6533076B2 (ja) 2014-03-26 2019-06-19 アークレイ株式会社 還元力の分析方法および還元力の分析試薬
US9588123B2 (en) 2014-04-11 2017-03-07 Surmodics Ivd, Inc. Compositions and methods for in vitro diagnostic tests including zwitterionic solubilization reagent
WO2016154250A1 (fr) 2015-03-23 2016-09-29 Surmodics Ivd, Inc. Agents bloquants hétérophiles pour immunoessais
CN108181245B (zh) * 2017-12-07 2020-12-08 中国农业科学院北京畜牧兽医研究所 酶法定量测定乳中乳过氧化物酶活性的方法及其试剂盒
WO2020217175A1 (fr) * 2019-04-26 2020-10-29 3M Innovative Properties Company Indicateur chimique de peroxyde d'hydrogène
CN111505274B (zh) * 2020-04-28 2023-06-20 武汉生命起源科技有限公司 一种用于酶联免疫反应的单组份tmb显色液的制备方法
CN112345475B (zh) * 2020-11-11 2022-09-20 昆明理工大学 一种快速检测食品中亚硝酸盐的方法
CN119630965B (zh) * 2023-07-18 2026-02-03 日东纺绩株式会社 TRACP-5b的测定方法和用于该方法的试剂盒

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE29498E (en) 1972-05-12 1977-12-20 Istituto Sieroterapico e Vaccinogeno Toscano "SCLAVO", S.p.A. Process for the enzymatic determination of glucose with a glucose-oxydazed/peroxidazed enzyme system
JPS58175496A (ja) * 1982-04-08 1983-10-14 Toyobo Co Ltd 基質または酵素活性の定量方法
US4778757A (en) * 1982-04-08 1988-10-18 Toyo Boseki Kabushiki Kaisha Method for the determination of substrates or enzyme activities
JPS6264955A (ja) * 1985-09-18 1987-03-24 Dai Ichi Pure Chem Co Ltd 発色の安定化法
JP2585723B2 (ja) * 1988-06-15 1997-02-26 コニカ株式会社 検出系の反応停止方法
US5206006A (en) 1989-08-31 1993-04-27 Instrumentation Laboratory Spa Stabilized trinder reagent
US5976896A (en) 1994-06-06 1999-11-02 Idexx Laboratories, Inc. Immunoassays in capillary tubes
AU754237B2 (en) * 1998-08-06 2002-11-07 Syntron Bioresearch, Inc. Uric acid assay device with stabilized uricase reagent composition
US6376252B1 (en) 1999-08-19 2002-04-23 Environmental Test Systems, Inc. Preparation and method for cat box filler additive capable of detecting feline lower urinary tract disease (FLUTD)
US6872828B2 (en) * 2001-12-20 2005-03-29 Lumigen, Inc. Compounds for generating chemiluminescence with a peroxidase
US7560556B2 (en) 2001-12-20 2009-07-14 Lumigen, Inc. Assay methods using chemiluminescent detection of peroxidase
GB0208989D0 (en) 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring enzyme activity
US20040219620A1 (en) 2003-04-29 2004-11-04 Mayer Brent A. Chromogenic substrate with a pH indicator dye
IT1400876B1 (it) * 2010-07-06 2013-07-02 Cyanagen S R L Metodo per incrementare e regolare l'emissione di luce da una reazione chemiluminescente.
CA2865254C (fr) * 2012-03-23 2020-10-13 Surmodics, Inc. Compositions et procedes pour tests de diagnostic in vitro comportant de l'acide sulfonique

Also Published As

Publication number Publication date
EP2841592B1 (fr) 2017-02-22
EP2841592A1 (fr) 2015-03-04
US20130252261A1 (en) 2013-09-26
CA2865254A1 (fr) 2013-09-26
EP2841592B8 (fr) 2017-05-03
US8927226B2 (en) 2015-01-06
US20140234874A1 (en) 2014-08-21
JP6437430B2 (ja) 2018-12-12
JP2015519038A (ja) 2015-07-09
WO2013142758A1 (fr) 2013-09-26
US9229005B2 (en) 2016-01-05

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