CA2950582C - Derivation sans cellules nourricieres, de cellules souches pluripotentes induites humaines, au moyen d'arn messager synthetique - Google Patents

Derivation sans cellules nourricieres, de cellules souches pluripotentes induites humaines, au moyen d'arn messager synthetique Download PDF

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CA2950582C
CA2950582C CA2950582A CA2950582A CA2950582C CA 2950582 C CA2950582 C CA 2950582C CA 2950582 A CA2950582 A CA 2950582A CA 2950582 A CA2950582 A CA 2950582A CA 2950582 C CA2950582 C CA 2950582C
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cells
reprogramming
somatic cells
reprogramming factor
feeder
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CA2950582A1 (fr
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Jiwu Wang
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Allele Biotechnology and Pharmaceuticals Inc
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Allele Biotechnology and Pharmaceuticals Inc
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Priority claimed from US14/292,317 external-priority patent/US10119150B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Transplantation (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne généralement de nouvelles méthodes et compositions permettant d'utiliser des facteurs de reprogrammation d'ingénierie génétique pour la création de cellules souches pluripotentes induites (iPSCs) par un processus régulé cinétiquement. Spécifiquement, cette invention se rapporte à l'établissement de combinaisons de facteurs de reprogrammation, y compris des fusions entre facteurs de reprogrammation classiques et des domaines de transactivation, optimisées pour la reprogrammation de divers types de cellules. Plus spécifiquement, les méthodes citées à titre d'exemple peuvent être utilisées pour créer des cellules souches pluripotentes induites à partir de divers types de cellules de mammifères, y compris des fibroblastes humains. L'invention porte également sur des méthodes représentatives de production sans cellules nourricières de cellules souches pluripotentes induites humaines, au moyen d'ARN messager synthétique.
CA2950582A 2014-05-30 2015-05-29 Derivation sans cellules nourricieres, de cellules souches pluripotentes induites humaines, au moyen d'arn messager synthetique Active CA2950582C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/292,317 2014-05-30
US14/292,317 US10119150B2 (en) 2012-05-13 2014-05-30 Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
PCT/US2015/033275 WO2015184318A1 (fr) 2014-05-30 2015-05-29 Dérivation sans cellules nourricières, de cellules souches pluripotentes induites humaines, au moyen d'arn messager synthétique

Publications (2)

Publication Number Publication Date
CA2950582A1 CA2950582A1 (fr) 2015-12-03
CA2950582C true CA2950582C (fr) 2020-09-22

Family

ID=54699891

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2950582A Active CA2950582C (fr) 2014-05-30 2015-05-29 Derivation sans cellules nourricieres, de cellules souches pluripotentes induites humaines, au moyen d'arn messager synthetique

Country Status (7)

Country Link
KR (1) KR102361849B1 (fr)
CA (1) CA2950582C (fr)
IL (1) IL249193B (fr)
PH (1) PH12016502343B1 (fr)
SG (1) SG11201609898WA (fr)
TW (1) TWI698526B (fr)
WO (1) WO2015184318A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12338462B2 (en) 2016-11-16 2025-06-24 Allele Biotechnology And Pharmaceuticals, Inc. Induction of pancreatic beta cells by stem cell differentiation with RNA
KR102586507B1 (ko) 2016-11-16 2023-10-11 알렐 바이오테크놀로지 앤 파마슈티칼스, 인크. Ｒｎａ를 사용한 줄기 세포 분화에 의한 간세포의 유도
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010108126A2 (fr) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Compositions de reprogrammation et procédés d'utilisation de celles-ci
US9580689B2 (en) * 2010-07-22 2017-02-28 Regents Of The University Of Minnesota Induced pluripotent stem cells
AU2013222188A1 (en) * 2012-02-22 2014-09-11 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA

Also Published As

Publication number Publication date
TW201600605A (zh) 2016-01-01
CA2950582A1 (fr) 2015-12-03
TWI698526B (zh) 2020-07-11
KR20170023014A (ko) 2017-03-02
PH12016502343B1 (en) 2023-02-01
SG11201609898WA (en) 2016-12-29
KR102361849B1 (ko) 2022-02-11
IL249193A0 (en) 2017-01-31
IL249193B (en) 2020-05-31
PH12016502343A1 (en) 2017-02-13
WO2015184318A1 (fr) 2015-12-03

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