TWI698526B - 利用合成之信使rna進行之人類誘導型多能幹細胞之無餵養細胞衍化(derivation) - Google Patents

利用合成之信使rna進行之人類誘導型多能幹細胞之無餵養細胞衍化(derivation) Download PDF

Info

Publication number
TWI698526B
TWI698526B TW104117573A TW104117573A TWI698526B TW I698526 B TWI698526 B TW I698526B TW 104117573 A TW104117573 A TW 104117573A TW 104117573 A TW104117573 A TW 104117573A TW I698526 B TWI698526 B TW I698526B
Authority
TW
Taiwan
Prior art keywords
cells
reprogramming
mrna
cell
feeder
Prior art date
Application number
TW104117573A
Other languages
English (en)
Chinese (zh)
Other versions
TW201600605A (zh
Inventor
繼武 王
Original Assignee
美商綠陽生物科技及製藥公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/292,317 external-priority patent/US10119150B2/en
Application filed by 美商綠陽生物科技及製藥公司 filed Critical 美商綠陽生物科技及製藥公司
Publication of TW201600605A publication Critical patent/TW201600605A/zh
Application granted granted Critical
Publication of TWI698526B publication Critical patent/TWI698526B/zh

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48Reproductive organs
    • A61K35/54Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/98Xeno-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/602Sox-2
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/603Oct-3/4
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/604Klf-4
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/605Nanog
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Transplantation (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
TW104117573A 2014-05-30 2015-05-29 利用合成之信使rna進行之人類誘導型多能幹細胞之無餵養細胞衍化(derivation) TWI698526B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/292,317 2014-05-30
US14/292,317 US10119150B2 (en) 2012-05-13 2014-05-30 Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA

Publications (2)

Publication Number Publication Date
TW201600605A TW201600605A (zh) 2016-01-01
TWI698526B true TWI698526B (zh) 2020-07-11

Family

ID=54699891

Family Applications (1)

Application Number Title Priority Date Filing Date
TW104117573A TWI698526B (zh) 2014-05-30 2015-05-29 利用合成之信使rna進行之人類誘導型多能幹細胞之無餵養細胞衍化(derivation)

Country Status (7)

Country Link
KR (1) KR102361849B1 (fr)
CA (1) CA2950582C (fr)
IL (1) IL249193B (fr)
PH (1) PH12016502343B1 (fr)
SG (1) SG11201609898WA (fr)
TW (1) TWI698526B (fr)
WO (1) WO2015184318A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12338462B2 (en) 2016-11-16 2025-06-24 Allele Biotechnology And Pharmaceuticals, Inc. Induction of pancreatic beta cells by stem cell differentiation with RNA
KR102586507B1 (ko) 2016-11-16 2023-10-11 알렐 바이오테크놀로지 앤 파마슈티칼스, 인크. Rna를 사용한 줄기 세포 분화에 의한 간세포의 유도
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012012708A1 (fr) * 2010-07-22 2012-01-26 Regents Of The University Of Minnesota Cellules souches pluripotentes induites

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010108126A2 (fr) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Compositions de reprogrammation et procédés d'utilisation de celles-ci
AU2013222188A1 (en) * 2012-02-22 2014-09-11 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012012708A1 (fr) * 2010-07-22 2012-01-26 Regents Of The University Of Minnesota Cellules souches pluripotentes induites

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rosa A1, Brivanlou AH.,Synthetic mRNAs: powerful tools for reprogramming and differentiation of human cells.,Cell Stem Cell.2010 Nov 5;7(5):p.549-550. *

Also Published As

Publication number Publication date
TW201600605A (zh) 2016-01-01
CA2950582C (fr) 2020-09-22
CA2950582A1 (fr) 2015-12-03
KR20170023014A (ko) 2017-03-02
PH12016502343B1 (en) 2023-02-01
SG11201609898WA (en) 2016-12-29
KR102361849B1 (ko) 2022-02-11
IL249193A0 (en) 2017-01-31
IL249193B (en) 2020-05-31
PH12016502343A1 (en) 2017-02-13
WO2015184318A1 (fr) 2015-12-03

Similar Documents

Publication Publication Date Title
US10435711B2 (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
AU2019203335B2 (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US10155929B2 (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
TWI698526B (zh) 利用合成之信使rna進行之人類誘導型多能幹細胞之無餵養細胞衍化(derivation)
JP2019170405A (ja) 合成メッセンジャーrnaによるヒト人工多能性幹細胞のフィーダーフリー誘導
HK40015744B (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
HK40015744A (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
HK40013817A (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
HK1204005B (zh) 使用合成的信使rna无饲养细胞地衍生人类诱导性多能干细胞
HK40013817B (en) Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna