CA2992462C - Silk performance apparel and products and methods of preparing the same - Google Patents

Abstract

Silk infused performance apparel and methods of preparing the same are disclosed herein. In some embodiments, silk performance apparel includes textiles, fabrics, consumer products, leather, and other materials that are coated with aqueous solutions of pure silk fibroin-based protein fragments. In some embodiments, coated apparel products, textiles, and upholstery, as well as other materials, exhibit surprisingly improved moisture management properties, resistance to microbial growth, increased abrasion resistance, and flame resistance.

Description

SILK PERFORMANCE APPAREL AND PRODUCTS AND METHODS OF PREPARING THE SAME CROSS-REFERENCE TO RELATED APPLICATIONS FIELD OF THEINVENTION In some embodiments, the invention relates to silk-coated performance apparel and products for use in home and automotive applications, such as fabrics or leather coated with pure silk fibroin-based proteins or protein fragments thereof.

BACKGROUND OF THE INVENTION Silk is a natural polymer produced by a variety of insects and spiders, and comprises a filament core protein, silk fibroin, and a glue-like coating consisting of a non-filamentous protein, sericin.

Silk fibers are light weight, breathable, and hypoallergenic, Silk is comfortable when worn next to the skin and insulates very well; keeping the wearer warm in cold temperatures and is cooler than many other fabrics in warm temperatures.

SUMMARY OF THE INVENTION Silk performance apparel and methods of preparing the same are disclosed herein According to aspects illustrated herein, the present disclosure relates to a product, including, but not limited to, apparel, padding, shoes, gloves, luggage, furs, jewelry and bags, configured to be worn or carried on the body, that is at least partially surface treated with a solution of pure silk fibroin-based protein fragments of the present disclosure so as to result in a silk coating on the product.

In some embodiments, the solutions of silk Date re?ue/Date received 2023-07-20 fibroin-based proteins or fragments thereof may be aqueous solutions, organic solutions, or emulsions.

In an embodiment, the product is manufactured from a textile material.

In an embodiment, the product is manufactured from a nan-textile material.

In an embodiment, desired additives can be added to an aqueous solution of pure silk fibroinbased protein fragments of the present disclosure so as to result in a silk coating having desired additives.

In an embodiment, a method is provided for coating a material with silk fibroin that may include silk-based proteins or fragments thereof to provide a silk fibroin coated material, wherein the silk fibroin coated upon Hie silk fibroin coated material may be heat resistant to a selected temperature.

In some embodiments, the method may include preparing a silk fibroin solution that may include a concentration of one or mare of low molecular weight silk fibroin, medium molecular weight silk fibroin, and high molecular weight silk fibroin at less than about 1% by volume (v/v), or less than about 0.1 % by volume (v/v), or less than about 0.01 % by volume (v/v), or less than about 0.001 % by volume (v/v).

In some embodiments, the method may include, coating a surface of the material with the silk fibroin solution.

In some embodiments, the method may include drying the surface of the material that has been coated with the silk fibroin solution to provide the silk fibroin coated material, wherein drying the surface of the material comprises heating the surface of the material without substantially decreasing silk fibroin coating performance.

In an embodiment, a method is provided for coating a textile with a silk fibroin solution that may include silk-based proteins or fragments thereof to provide a silk fibroin coated article, wherein the silk fibroin coated upon the silk fibroin coated article may be heat resistant to a selected temperature.

In some embodiments, the method may include preparing the silk fibroin solution with one or more of low molecular weight silk fibroin, medium molecular weight silk fibroin, and high molecular weight silk fibroin.

In some embodiments, the method may include acidically adjusting the pH of the silk fibroin solution with an acidic agent.

In some embodiments, the method may indude coating a surface of the textile with the silk fibroin solution.

In same embodiments, the method may include drying the surface of the textile that has been coated with the silk fibroin solution to provide the silk fibroin coated artide, wherein drying the surface of Date re?ue/Date received 2023-07-20 the textile comprises heating the surface of the textile without substantially decreasing silk fibroin coating performance.

In some embodiments, a method is provided for manufacturing a silk fibroin coated textile that may include selected fabric properties.

In some embodiments, the method may include admixing silk-based proteins or fragments thereof with one or more chemical agents to provide a coating solution, wherein the one or more chemical agents may be selected to modify one or more of a first selected property and second selected property of the silk fibroin coated textile.

In some embodiments, the method may include providing the coating solution to a textileto be coated with one or more of a bath coating process, a kiss rolling process, a spray process, and a two-sided rolling process.

In some embodiments, the method may indude removing excess coating solution from the silk fibroin coated textile.

In some embodiments, the method may include heating the silk fibroin coated textile to modify a third selected property of the silk fibroin coated textile.

In some embodiments, the first selected property may include one or more of an antimicrobial property, a water repelIant property, an oil repellant property, a flame retardant property, a coloring property, a fabric softening property, a stain repellant property, a pH adjusting property, an anticrocking property, an antipilling property, and an antifelting property.

In some embodiments, the second selected property may indude one or more of wetting time; absorption rate, spreading speed, accumulative one-way transport, and overall moisture management capability.

In some embodiments, the third selected property may include one or more of fabric hand, fabric stretch, and drapability.

In an embodiment, the silk fibroin coated materials of Ute invention may be coated with one or more of low molecular weight silk, medium molecular weight silk, and high molecular weight silk to provide resulting coated materials having enhanced hydrophobic or hydrophilic properties.

In and embodiment, materials coated by silk fibroin coatings described herein may include one or more of textiles, woven materials, non-woven materials, knit materials, crochet materials, and leather materials.

Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight averagemolecular weight range of about 5 kDa toabout 144 kDa.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having an average number of amino acid residues of about 1to 400 residues, or 1 to 300 residues, or 1 to 200 residues, or 1 to 100 residues, or 1 to 50 residues, or 5 to 25 residues, or 10 to 20 residues.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein thearticleis afabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-basedproteins orproteinfragments havingabout 0.01%(w/w)to about 10% (w/w) sericin.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinationsthereof.

In an embodiment, die invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected Date regue/Date received 2023-07-20 from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein Ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and tite silkw orm silk based proteins or fragments thereof is Bombyx mori silk based proteins orfragments thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins ar protein fragments thereof have an average weight average molecular weight range selected from the group consisting of about 5 toabout 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gdate and do not visibly change in color or turbidity when in a solution for at least 10 days. 55 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, syntheticfiber or yarn, or combinations thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinationsthereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein die fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester** polyurethane copolymer, and combinations thereof.

In an embodiment, die invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the fabric exhibits an improved property, wherein the improved property is an accumulative one-way moisture transport index selected from the group consisting of greater than 40 %, greater than 60 %, greater than 80 %, greater than 100 %, greater than 120 %, greater than 140 %, greater than 160 %, and greater than 180%.

In an embodiment, the foregoing improved property is determined after a period of machine washing cycles selected from the group consisting of 5 cydes, 10 cycles, 25 cydes, and 50 cycles. 65 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the fabric exhibits animproved property, wherein the improved property is an accumulative one way transport capability increase relative to uncoated fabric selected from the group consisting of 1.2 fold, 1.5 fold, 2.0 fold, 3.0 fold, 4.0 fold, 5.0 fold, and 10 fold.

In an embodiment, the foregoing improvedproperty is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the fabric exhibits an improved property, wherein the improved property is an overall moisture management capability selected from the group consisting of greater than 0.05, greater than 0.10, greater than 0.15, greater than 0.20, greater than 0.25, greater than 0.30, greater than 0.35, greater than0.40, greater than 0.50, greater than 0.60, greater than 0.70, and greater than 0.80.

In an embodiment, the foregoing improved property is determined after a period of machine washing cycles selected fiom the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric exhibits substantially no increase in microbial growth after a number of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die article is a fabric, wherein the fabric exhibits substantially no increase in microbial growth after a number of machine washing cycles selected from die group 75 Bate re?ue/Date received 2023-07-20 consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles, and wherein the microbial growth is microbial growth of a microbe selected from the group consisting of Staphylococcusaureus,Klebisiella pneumoniae, and combinationsthereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the fabric exhibits substantially no increase in microbial growth after a number of machine washing cycles selected from the group consisting cf 5 cycles, 10 cycles, 25 cycles, and 50 cycles, wherein the microbial growth is microbial growth a microbe selected from the group consisting of Staphylococcus aureus, Klebisiella pneumoniae, and combinations thereof, wherein the microbial growth is reduced by a percentage selected from tire group consisting of 50 %, 100 %, 500 %, 1000 %, 2000 %, and 3000 % compared toan uncoated fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the coating is applied to the fabric at the fiber level priorto forming the fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the coating is applied tothe fabric at the fabric level.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to 1he fabric at the fabric level, and wherein thefabricis bath coated.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 85 Date re?ue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein thefabricis spray coated.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein the fabric is coated with a stencil.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to1he fabric at 1he fabric level, wherein the coating is applied to at least one side of the fabric using a method selected from the group consisting of a bath coating process, a spray coating process, a stencil process, a silk-foam based process, and a roller-based process.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein the coating has a thickness of about one nanolayer.

In an embodiment, the invention provides an article comprising a flbar or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein die coating has a thickness selected from the group consisting of about 5 nm, about 10 nm, about 15 nm, about 20 nm, about 25 nm, about 50 nm, about 100 nm, about 200 nm, about 5OO nm, about1 pm, about 5 pm, about 10 pm, and about 20 pm.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is afabric, and wherein the (matingis adsorbed on the fabric. 95 Date regue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the coating is attached to the fabric through chemical, enzymatic, thermal, or irradiative cross-linking.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein the hand of the coated fabric is improved relative to an uncoated fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein the hand of the coated fabric is improved relative to an uncoated fabric, wherein the hand of the coated fabric that is improved is selected from the group consistingof softness, crispness, dryness, silkiness, and combinations thereof.

According to aspects illustrated herein, an aqueous solution of pure silk fibroinbased protein fragments of the present disclosure is available for application toa product, including, but not limited to, apparel, padding, shoes, gloves, luggage, furs, jewelry and bags, or for directly spraying on the body of a consumer, to impart desired properties to the product.

In an embodiment, the product is manufactured from a textile material.

In an embodiment, the product is manufactured from a non-textile material.

In an embodiment, desired additives can be added to an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure so as to result in a silk coating having desired additives.

In an embodiment, a textile comprising a silk coating of the present disclosure is sold to a consumer.

In an embodiment, a textile of the present disclosure is used in constructing action sportswear apparel.

In an embodiment, a textile of the present 105 Date re?ue/Date received 2023-07-20 disclosure is used in constructing fitness apparel.

In an embodiment, a textile of the present disclosure is used in constructing performance apparel.

In an embodiment, a textile of the present disclosure is used in constructing golf apparel.

In an embodiment, a textile of tiie present disclosure is used in constructing lingerie.

In an embodiment, a silk coating of the present disclosure is positioned on the underlining of action sportswear/app.'wel.

In an embodiment, a silk coating of the present disclosure is positioned on the shell, the lining, or the interlining of action sportswear/apparel.

In an embodiment, action sportswear/apparel is partially made from a silk coated textile of the present disclosure and partially made from an uncoated textile.

In an embodiment, action sportswear/apparel partially made from a silk coated textile and partially made from an uncoated textile combines an uncoated inert synthetic material with a silk coated inert synthetic material.

Examples of inert synthetic material indude, but are not limited to, polyester, polyamide, polyaramid, polytetrafluorethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethylenglycol, ultrahigh molecular weight polyethylene, high-performance polyethylene, nylon, LYCRA* (polyester-polyurethane copolymer, also known as SPANDEX and elastomer), and mixtures thereof.

In an embodiment, action sportswear/apparel partially made from a silk coated textile and partially made from an uncoated textile combines an elastomeric material at least partially covered with a silk coating of the present disclosure.

In an embodiment, the percentage of silk to elastomeric material can be varied to achieve desired shrink or wrinkle resistant properties and desired moisture content against the skin surface.

In an embodiment, a silk coating of the present disclosure is positioned on an internal layer of a shoe (textile or non-textile based).

In an embodiment, a silk coating of the present disclosure positioned on an internal layer of a shoe helps maintain optimal feet microenvironment such as temperature and humidity while reducing any excessive perspiration.

In an embodiment, a silk coating of the present disclosure is visible.

In an embodiment, a silk coating of the present disclosure is transparent In an embodiment, a silk coating of the present disclosure positioned on action sportswear/apparel helps control skin temperature of a person wearing the apparel.

In an embodiment, a silk coating of the present disclosure positioned on action sportswear/apparel helps control 115 Date re?ue/Date received 2023-07-20 fluid transfer away from the skin of a person wearing the apparel.

In an embodiment, a silk coaling of the present disclosure positioned on action sportswear/apparel has a soft feel against the skin decreasing abrasions from fabric on the skin.

In an embodiment, a silk coating of die present disclosure positioned on a textile has properties that confer at least one of wrinkle resistance, shrinkage resistance, or machine washability to the textile.

In an embodiment, a silk coated textile of the present disclosure is 100% machine washable and dry cleanable.

In an embodiment, a silk coated textile of dm present disclosure is 100% waterproof In an embodiment, a silk coated textile of the present disclosure is wrinkle resistant.

In an embodiment, a silk coated textile of the present disclosure is shrink resistant.

In an embodiment, a silk coated fabric improves die health of the skin.

In an embodiment, healthy skin can be determined by visibly seeing an even skin tone, hi an embodiment, healthy skin can be determined by visibly seeing a smooth, glowing complexion.

In an embodiment, a silk coated fabric decreases irritation of the skin.

In an embodiment, a decrease in irritation of the skin can result in a decrease in skin bumps or sores.

In an embodiment, a decrease in irritation of the skin can result in a decrease in scaly or red skin.

In an embodiment, a decrease in irritation of the skin can result in a decrease in itchiness or burning.

In an embodiment; a silk coated fabric decreasesinflammation of the skin.

In an embodiment, a silk coated textile of tire present disclosure has the qualities of being waterproof, breathable, and elastic and possess a number of other qualities which are highly desirable in action sportswear.

In an embodiment, a silk coated textile of the present disclosure manufactured from a silk fabric of1he present disclosure further includes LYCR/^brand spandex fibers (polyester¬ polyurethane copolymer) In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a breathable fabric.

In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a water-resistant fabric.

In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a shrink-resistant fabric.

In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a machine-washablefabric.

In 125 Date re?ue/Date received 2023-07-20 an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the prosent disclosure is a wrinkle resistant fabric.

In an embodiment, textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure provides moisture and vitamins to the skin.

Inan embodiment, thetextile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has an accumulative one-way transport index of greater than 140.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has an accumulative one-way transport index of greater than 120.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has an accumulative one¬ way transport index of greater than 100.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosurehas an accumulative one-way transport index ofgreater than 80.

Inan embodiment, thetextile at least partially coated with an aqueous solution of pure silk fibroin-basedprotein fragmentsofthepresent disclosurehas an overall moisture management capability of greater than 0.4.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has an overall moisture management capability of greater than 0.35.

In an embodiment, the textile at least partially coated with an aqueous solution of pure sulk fibroin-basedprotein fragmentsof thepresent disclosurehas anoverallmoisture management capability of greater than 0.3.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-basedprotein fragments of the present disclosure has an overall moisture management capability of greater than 0.25.

Inan embodiment, thetextile at least partially coatedwith an aqueous solution of pure silkfibroin-basedproteinfragments of thepresent disclosurehas awettingtimeof at least 3 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a 135 Bate regWBate njKfeimi wetting time of at least 2.5 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosurehas a wetting time of at least 2 seconds.

In an embodiment, the textile at least partially coaled with an aqueous solution of pure silk fibroin-based protein fragmentsof the present disclosurehas awettingtimeof at least 1.5 seconds.

Inanembodiment, thetextile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a top absorption time of at least 50 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a top absorption time of at least 40 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosurehas a top absorptiontimeof at least 30 seconds.

Inan embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a bottom absorption time of at least 80 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a bottom absorption time of at least 70 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of tire present disclosure has a bottom absorption time of at least 60 seconds.Inan embodiment, thetextile at least partially coatedwith an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a bottom absorption time of at least 50 seconds.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosurehas abottom absorption time ofat least 40 seconds.

Inan embodiment, thetextile at least partially coated with an aqueous solutionof pure silk fibroin-based protan fragments of the present disclosure has a spreading speed of atleast 1.6 mm/second, Inan embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a spreading speed of at least 1.4 mm/second.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of 145 Date re?ue/Date received 2023-07-20 the present disclosure has a spreading speed of at least 1.2 mm/second.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a spreading speed of at least 1.0 mm/second.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure has a spreading speed of at least 0.8 mm/second.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 2000% microbial growth over 24 hours.

In an embodiment, fire textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure show's less than 1000% microbial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 500% microbial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 400% microbial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 300% microbial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 200% microbial growth over 24 hours.

In some embodiments, as described herein, the reduction in microbial growth may be measured and provided after one or more wash cycles in non¬ chlorine bleach, hi some embodiments, solutions that include silk fibroin-based protein fragments may include an additional chemical agent, as described herein, that may provide antimicrobrial (e g., antifungal and/or antibacterial) properties.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 2000% bacterial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 1000% bacterial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein 155 Date re?ue/Date received 2023-07-20 fragments of the present disclosure shows less than 500% bacterial growth over 24 hairs.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 400% bacterial growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 300% bacterial growth over 24 hours.

In an embodiment, the textile at least partially coatedwith an aqueous solution of pure silk fibroin-basedprotein fragmentsof the present disclosure showsless than 200% bacterial growth over24 hours.

Inan embodiment, thetextile at least partially coatedwithan aqueous solution of pure silk fibroin-basedprotein fragmentsof thepresent disclosure showsless than 2000% fungal growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution ofpure silk fibroin-basedprotein fragments ofthe present disclosure shows less than 1000% fungal growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 500% fungal growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 400% fungal growth over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution ofpure silk fibroin-based protein fragments ofthe present disclosure shows lessthan300% fungalgrowth over 24 hairs.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 200% fungal growth over 24hours.

Inan embodiment, thetextile at least partially coatedwith an aqueous solution of pure silk fibroin-basedprotein fragments of thepresent disclosure showsless than 2000% growth ofStaphylococcus aureus over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 1000% growth of Staphylococcus aureus over 24 hours.

Inan embodiment, the textile at least partially coated with an aqueous solution of pure silk,fibroin-hasedprotein fragments of the present disclosure shows less than 500% growth ofStaphylococcus aureus over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of 165 Date re?ue/Date received 2023-07-20 the present disclosure shows less than 400% growth of Staphylococcus aureus over 24 hours.

Inan embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 300% growth ofStaphylococcus aureus over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 200% growth of Staphylococcus aureus over 24 hours.

Inan embodiment, thetextile at least partially coatedwith an aqueous solution of pure silk fibroin-basedprotein fragmentsof thepresent disclosure showsless than 2000% growth ofKlebsiella pneumoniae over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 1000% growth of Klebsiellapneumoniae over 24 hours.

Inan embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-basedprotein fragments of the present disclosure shows less than 500% growth ofKlebsiella pneumoniae over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less tihan 400% growth of Klebsiellapneumoniae over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-basedprotein fragments of the present disclosure shows less than 300% growth ofKlebsiellapneumoniae over 24 hours.

In an embodiment, the textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure shows less than 200% growth of Klebsiella pneumoniae over 24 hours.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is used to coat a textile.

In an embodiment, the concentration of silk in the solution ranges from about 0.001% to about 20.0%.

In an embodiment, the concentration of silk in the solution ranges from about 0.01% to about 15.0%.

In an embodiment, the concentration of silk in the solution ranges from about 0.5% to about 10.0%.In an embodiment, the concentration of silkin the solution ranges from about 1.0% to about 5.0%.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is applied directly to a fabric. 175 Date re?ue/Date received 2023-07-20 Alternatively. silk mioosphae and any additives may be used for coating a fabric.

In an embodiment, additives can be added to an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure before coating (e.g., alcohols) to further enhance material properties.

In an embodiment, a silk coating of 1he present disclosure can have a pattern to optimize properties of the silk on the fabric.

In an embodiment, a coating isapplied to a fabric under tension and/or lax to vary penetration into thefabric.

In an embodiment, a silk coating of the present disclosure can be applied at the yam level, followed by creation ofa fabric once the yam is coated.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure can be spun into fibers to make a silk fabric and/or silk fabric blend with other materials known in the apparel industry.

In an embodiment, a method for silk coating a fabric includes immersion of the fabric in any of the aqueous solutions of pure silk fibroin-based protein fragments of the present disclosure.

In an embodiment, a method for silk coating a fabric includes spraying.

In an embodiment, a method for silk coating a fabric includes chemical vapor deposition.

In an embodiment, a method for silk coating a fabric includes electrochemical coating.

In an embodiment, a method for silk coating a fabric includes knife coating to spread any of the aqueous solutions of pure silk fibroin-based protein fragments of the present disclosure onto the fabric.

The coated fabric may then be air dried, dried under heat/air flow, or cross-linked to the fabric surface.

In an embodiment, a (hying process includes curing with additives and/or ambient condition.

According to aspects illustrated herein, methods for preparing aqueous solutions of pure silk fibroin-based protein fragments are disclosed.

In an embodiment, at least one pure silk fibroin-based protein fragment (SPF) mixture solution having a specific average weight average molecular weight (MW) range and polydispersity is created.

In an embodiment, at least SPF mixture solution having a MW range between about 6 kDa and 16 kDa and a polydispersity range between about 1.5 and about 3.0 is created.

In an embodiment, at least one SPF mixture solution having a MW between about 17 kDa and 38 kDa and a polydispersity range between about 1.5 and about 3.0 is created.

In an 185 Date re?ue/Date received 2023-07-20 embodiment, at least one SPF mixture solution having a MW range between about 39 kDa and 80 kDa and a polydispersity range between about 1.5 and about 3.0 is created.

According to aspects illustrated herein, there is disclosed a composition that includes pure silk fibrain-based protein fragments that are substantially devoid of sericin, wherein the composition has an average weight average molecular weight ranging from about 6 kDa to about 16 kDa, wherein die composition has a polydispersity of between about 1.5 and about 3.0, wherein the composition is substantially homogenous, wherein the composition includes between 0 ppm and about 500 ppm of inorganic residuals, and wherein the composition includes between 0 ppm and about 500 ppm of organic residuals.

In an embodiment, the pure silk fibroin-based protein fragments have between about 10 ppm and about 300 ppm of lithium bromide residuals and between about 10 ppm and about 100 ppm of sodium carbonate residuals.

In an embodiment, the lithium bromide residuals are measurable using a high-performance liquid chromatography lithium bromide assay, and the sodium carbonate residuals are measurable using a highperformance liquid chromatography sodium carbonate assay.

In an embodiment, the composition further includes less than 10% water.

In an embodiment, the composition is in the form of a solution.

In an embodiment, the composition indudes from about 0.01 wt %to about 30.0 wt % pure silk fibroin-based protein fragments.

The pure silk fibroinbased protein fragments are stable in the solution for at least 30 days.

In an embodiment, the term “stable” refers to the absence of spontaneous or gradual gelation, with no visible change in the color or turbidity of the solution.

In an embodiment, the term “stable” refers to no aggregation of fragments and therefore no increase in molecular weight over time.

In an embodiment, the composition is in the form of an aqueous solution.

In an embodiment, the composition is in the form of an organic solution.

The composition may be provided in a sealed container.

In some embodiments, the composition further includes one or more molecules selected from the group consisting of therapeutic agents, growth factors, antioxidants, proteins, vitamins, carbohydrates, polymers, nucleic adds, salts, acids, bases, biomolecules, glycosamino glycans, polysaccharides, extracellular matrix molecules, metals, metal ion, metal oxide, synthetic molecules, polyanhydrides, cells, fatty acids, fragrance, minerals, plants, plant extracts, preservatives and essential oils.

In an embodiment, the added molecule or molecules are stable (i.e., retain activity over 195 Date re?ue/Date received 2023-07-20 time) within the composition and can be released at a desired rate.

In an embodiment, the one or more molecules is vitamin C or a derivative thereof.

In an embodiment, the composition further includes an alpha hydroxy acid selected from the group consisting of glycolic add, lactic acid, tartaric acid and citric acid, hi an embodiment, the composition further indudes hyaluronic add or its salt form at a concentration of about 0.5% to about 10.0%.

In an embodiment, the composition further indudes at least one of zinc oxide or titanium dioxide.

In an embodiment, the pure silk fibroin-based protein fragments in the composition are hypoallergenic.

In an embodiment, the pure silk fibroin-based protein fragmentsare biocompatible, non-sensitizing, and non-immunogenic.

According to aspects illustrated herein, there is disclosed a composition that includes pure silk fibroin-based protein fragments that are substantially devoid of seridn, wherein the composition has an average weight average molecular weight ranging from about 17 kDa to about 38 kDa, wherein the composition has a polydispersity of between about 1.5 and about 3.0, wherein the composition is substantially homogenous, wherein the composition includes between 0 ppm and about 500 ppm of inorganic residuals, and wherein the composition includes between 0 ppm and about 500 ppm of organic residuals.

In an embodiment, the pure silk fibroin-based protein fragments have between about 10 ppm and about 300 ppm of lithium bromide residuals and between about 10 ppm and about 100 ppm of sodium carbonate residuals.

In an embodiment, the lithium bromide residuals are measurable using a high-performance liquid chromatography lithium bromide assay, and the sodium carbonate reriduals are measurable using a highperformance liquid chromatography sodium carbonate assay.

In an embodiment, the composition further indudes less than 10% water.

In an embodiment, the composition is in the form of a solution.

In an embodiment, the composition indudes from about 0.01 wt %to about 30.0 wt% pure silk fibroin-based protein fragments.

The pure silk fibroinbased protein fragments are stable in the solution for at least 30 days.

In an embodiment, the term “stable” refers to the absence of spontaneous or gradual gelation, with no visible change in the color or turbidity of the solution.

In an embodiment, the term “stable” refers to no aggregation of fragments and therefore no increase in molecular weight over time.

In an embodiment, the composition is in the form of an aqueous solution, hi an embodiment, the composition is in the form of an organic solution.

The composition may 205 Date re?ue/Date received 2023-07-20 be provided in a sealed container.

In some embodiments, the composition further includes one or more molecules selected from the group consisting of therapeutic agents, growth factors, antioxidants, proteins, vitamins, carbohydrates, polymere, nucleic adds, salts, acids, bases, biomolecules, glycosamino glyeans, polysaccharides, extracellular matrix molecules, metals, metal ion, metal oxide, synthetic molecules, polyanhydrides, cells, fatty acids, fragrance, minerals, plants, plant extracts, preservatives and essential oils.

In an embodiment, the added molecule or molecules are stable (i.e., retain activity over time) within the composition and can be released at a desired rate, hi an embodiment, the one or more molecules is vitamin C or a derivative thereof.

In an embodiment, the composition further indudes an alpha hydroxy acid selected from the group consisting of glycolic acid, lactic acid, tartaric acid and citric add.

In an embodiment, the composition further includes hyaluronic acid or its salt form at a concentration of about 0.5% to about 10.0%.

In an embodiment, the composition further includes at least one of zinc oxide or titanium dioxide.

In an embodiment, the pure silk fibroin-based protein fragments in the composition are hypoallergenic.

In an embodiment, the pure silk fibroin-based protein fragments are biocompatible, non¬ sensitizing, and non-immunogenic.

According to aspects illustrated herein, there is disclosed a compostion that includes pure silk fibroin-based protein fragments that are substantially devoid of sericin, wherein die composition has an average weight average molecular weight ranging from about 39 kDa to about 80 kDa, wherein the composition has a polydispersity of between about 1.5 and about 3.0, wherein die composition is substantially homogenous^ wherein the composition includes between 0 ppm and about 500 ppm of inorganic residuals, and wherein die composition includes between 0 ppm and about 500 ppm of organic residuals.

In an embodiment, the pure silk fibroin-based protein fragments have between about 10 ppm and about 300 ppm of lithium bromide residuals and between about 10 ppm and about 100 ppm of sodium carbonate residuals.

In an embodiment, die lithium bromide residuals are measurable using a high-performance liquid chromatography lithium bromide assay, and the sodium carbonate residuals are measurable using a highperformance liquid chromatography sodium carbonate assay.

In an embodiment, the composition further includes less than 10% water.

In an embodiment, the composition is 215 Date re?ue/Date received 2023-07-20 in the form of a solution.

In an embodiment, the composition includes from about 0.01 wt%to about 30.0 wt%pure silk fibroin-basedprotein fragments.

Thepure silk fibroinbasedprotein fragments are stablein the solution for at least 30 days.

In an embodiment, the term “stable” refers to the absence of spontaneous or gradual gelation, withno visible change in die odor or turbidity of the solution.

In an embodiment, the term “stable” refers to no aggregation of fragments and therefore no increase inmolecular weight over time.

In an embodiment, the composition is in the form of an aqueous solution.

In an embodiment, the compositionisinthe form ofan organic solution.

The compoationmay be providedin a sealedcontainer.In some embodiments, the compostionfurtherincludes one or more molecules selected from the group consisting of therapeutic agents, growth factors, antioxidants, proteins, vitamins, carbohydrates, polymers, nucleic adds, salts, acids, bases, biomolecules, glycosamino glycans, polysaccharides, extracellular matrix molecules, metals, metal ion, metal oxide, synthetic molecules, polyanhydrides, cells, fatty acids, fragrance, minerals, plants, plant extracts, preservatives and essential oils.

In an embodiment, the added molecule or molecules are stable (i.e., retain activity over time) within the composition and can bereleased at a desired rate.In an embodiment, the one or more molecules is vitamin C or a derivative thereof.

In an embodiment, the composition further indudes an alpha hydroxy acid sdected from the group consisting of glycolic arid, lactic acid, tartaric arid and citric acid.In an embodiment, the composition further indudeshyaluronic arid orits salt form at a concentration of about 0.5%to about 10.0%.

In an embodiment, the composition further indudes at least one of zinc oxide or titanium dioxide.

In an embodiment, the pure silk fibroin-based protein fragmentsin the composition are hypoallergenic, hi an embodiment, the pure silk fibroin-based protein fragments are biocompatible,non-sensitizing, andnon-immunogenic.

According to aspects illustrated herein, there is disclosed a gel that indudes pure silk fibroin-based protein fragments substantially devoid of sericin and comprising: an average weight average molecular weight ranging from about 17 kDa to about 38 kDa; and a polydispersity ofbetween about 1.5 and about 3.0; and water from about 20 wt % to about 99.9 wt %, wherein the gel includes between 0 ppm and 500 ppm ofinorganic residuals, and wherein the gd includes between 0 ppm and 500 ppm of organic residuals.

In an embodiment, the gel includes between about 1.0% and about 50.0% crystalline 225 Date re$ue/Date received 2023-07-20 protein domains.

In an embodiment, the gel indudes from about O.l wt. % to about 6.0 wt. % of pure silk fibroin-based protein fragments In an embodiment, the gel has a pH from about 1.0 to about 7.0.

In an embodiment, the gel further includes from about 0.5 wt. % to about 20.0 wt. % of vitamin C or a derivative thereof.

In an embodiment, the vitamin C or a derivative thereof remains stable within the gel for a period of flora about 5 days to about 5 years.

In an embodiment, the vitamin C or a derivative thereof is stable within the gel so as to result in release of the vitamin C in a biologically active form.

In an embodiment, the gd further includes an additive selected from the group consisting of vitamin E, rosemary oil, rose oil, lemon juice, lemon grass oil and caffeine.

In an embodiment, the gel is packaged in an airtight container.

In an embodiment, the pure silk fibroin-based protein fragments are hypoallergenic.

In an embodiment, the gd has less than 10 colony forming units per milliliter.

According to aspects illustrated herein, there is disclosed a method for preparing an aqueous solution of pure silk fibroin-based protdn fragments having an average weight average molecular weight ranging from about 6 kDa to about 16 kDa, the method including the steps of: degumming a silk source by adding the silk source to a boiling (100°C) aqueous solution of sodium carbonate for a treatment time of between about 30 minutes to about 60 minutes; removing sericin from the solution to produce a silk fibroin extract comprising non-detectable levels of sericin; draining the solution from the silk fibroin extract; dissolving the silk fibroin extract in a solution of lithium bromide having a starting temperature upon placement of the silk fibroin extract in the lithium bromide solution that ranges from about 60°C to about 140°C; maintaining the solution of silk fibrom-lithium bromide in an oven having a temperature of about 140°C for a period of at least1 hour, removingthe lithium bromide from the silk fibroin extract; and producing an aqueous solution of silk protdn fragments, the aqueous solution comprising: fragments having an average weight average molecular weight ranging from about 6 kDa to about 16 kDa, and wherein die aqueous solution of pure silk fibroin-based protein fragments comprises a polydispersity of between about 1.5 and about 3.0.

In an embodiment, the method includes die step of drying die silk fibroin extract prior to die dissolving step.

In an embodiment, the amount of lithium bromide residuals in the aqueous solution can be measured using a high-performance liquid chromatography lithium bromide assay.

In an 235 Date re?ue/Date received 2023-07-20 embodiment, the amount of sodium carbonate residuals in the aqueous solution can be measured using a high-performance liquid chromatography sodium carbonate assay.

In an embodiment, die method includes the step of adding a therapeutic agent to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method includes the step of adding a molecule selected from one of an antioxidant or an enzyme to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method includes die step of adding a vitamin to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, die vitamin is selected from one of vitamin C or a derivative thereof.

In an embodiment, die method further includes the step of adding an alpha hydroxy arid todie aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the alpha hydroxy acid is selected from die group consisting of glycolic acid, lactic acid, tartaric acid and citric acid.

In an embodiment, the method further includesdie step of adding hyaluronic arid at a concentration of about 0.5%to about 10.0% tothe aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method further includes the step of adding at least one of zinc oxide or titanium dioxide to the aqueous solution of pure silk fibroin-based protein fragments.

According to aspects illustrated herein, there is disclosed a method for preparing an aqueous solution of pure silk fibroin-based protein fragments having an average weight average molecular weight ranging from about 17 kDa to about 38 kDa, the method including the steps of: adding a silk source to a boiling (100°C) aqueous solution of sodium carbonate for a treatment time of between about 30 minutes to about 60 minutes so as to result in degumming; removing seririn from the solution to produce a silk fibroin extract comprising non-detectable levels of seririn; draining the solution from the silk fibroin extract; dissolving the silk fibroin extract in a solution of lithium bromide having a starting temperature upon placement of the sulk fibroin extract in the lithium bromide solution that ranges from about 80°C to about 140°C; maintaining the solution of silk fibroin-lithium bromide in a dry oven having a temperature in the range between about 60°C to about 100°C for a period of at least 1 hour; removing the lithium bromide from the silk fibroin extract; and producing an aqueous solution of pure silk fibroin-based protein fragments, wherein the aqueous solution of pure silk fibroin-based protein 245 Date re?ue/Date received 2023-07-20 fragments comprises lithium bromide residuals of between about 10 ppm and about 300 ppm, wherein the aqueous solution of silk protein fragments comprises sodium carbonate residuals of between about 10 ppm and about 100 ppm, wherein die aqueous solution of pure silk fibroin-based protein fragments comprises fragments having an average weight average molecular weight ranging from about 17 kDa to about 38 kDa, and wherein the aqueous solution of pure silk fibroin-based protein fragments comprises a polydispersity of between about 1.5 and about 3.0.

In an embodiment, the method includes the step of drying the silk fibroin extract prior to the dissolving step.

In an embodiment, the amount of lithium bromide residuals in the aqueous solution can be measured using a highperformance liquid chromatography lithium bromide assay.

In an embodiment, the amount of sodium carbonate residuals in the aqueous solution can be measured using a high-performance liquid chromatography sodium carbonate assay.

In an embodiment, the method includes the step of adding a therapeutic agent to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method includes the step of adding a molecule selected from one of an antioxidant or an enzyme to the aqueous solution of pure silk fibroin-based protan fragments.

In an embodiment, the method includes the step of adding a vitamin to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the vitamin is selected from one of vitamin C or a derivative thereof.

In an embodiment, the method further includes the step of adding an alpha hydroxy acid to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the alpha hydroxy acid is selected from the group consisting of glycolic acid, lactic acid, tartaric acid and citric acid.

In an embodiment, the method further includes the step of adding hyaluronic acid at a concentration of about 0.5% to about 10.0% to die aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method further includes the step of adding at least one of zinc oxide or titanium dioxide to the aqueous solution of pure silk fibroin-based protein fragments.

According to aspects illustrated herein, there is disclosed a method for preparing an aqueous solution of pure silk fibroin-based protein fragments having an average weight average molecular weight ranging from about 39 kDa to about 80 kDa, the method including the steps of: adding a silk source to a boiling (100°C) aqueous solution of sodium carbonate for a treatment time of about 30 minutes so as to result in 255 Date re?ue/Date received 2023-07-20 degumming; removing sericin from the solution to produce a silk fibroin extract comprising non-detectable levels of sericin; draining the solution from the silk fibroin extract; dissolving the silk fibroin extract in a solution of lithium bromide having a starting temperature upon placement of the silk fibroin extract in the lithium bromide solution that ranges from about 80°C to about 140°C; maintaining the solution of silk fibroin-lithium bromide in a dry oven having a temperature in the range between about 60°C to about 100°C for a period of at least 1 hour; removing the lithium bromide from the silk fibroin extract; and producing an aqueous solution of pure silk fibroin-based proton fragments, wherein the aqueous solution of pure silk fibroin-based protein fragments comprises lithium bromide reriduals of between about 10 ppm and about 300 ppm, sodium carbonate reriduals of between about 10 ppm and about 100 ppm, fragments having an average weight average molecular weight ranging from about 40 kDa to about 65 kDa, and wherein the aqueous solution of pure silk fibroin-based protein fragments comprises a polydispersity of between about 1.5 and about 3.0.

In an embodiment, the method includes die step of drying the silk fibroin extract prior to the dissolving step.

In an embodiment, the amount of lithium bromide residuals in the aqueous solution can be measured using a high-performance liquid chromatography lithium bromide assay.

In an embodiment, the amount of sodium carbonate reriduals in the aqueous solution can be measured using a high-performance liquid chromatography sodium carbonate assay.

In an embodiment, the method includes the step of adding a therapeutic agent to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method includes the step of adding a molecule selected from one of an antioxidant or an enzyme to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the method includes file step of adding a vitamin to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the vitamin is selected from one of vitamin C or a derivative thereof.

In an embodiment, the method further includes the step of adding an alpha hydroxy acid to the aqueous solution of pure silk fibroin-based protein fragments.

In an embodiment, the alpha hydroxy acid is selected from the group consisting of glycolic acid, lactic acid, tartaric acid and citric acid.

In an embodiment, the method further includes the step of adding hyaluronic acid at a concentration of about 0.5% to about 10.0% to the aqueous solution of pure silk 265 Date repue/Date received 2023-07-20 fibroin-based protein fragments.

In an embodiment, the method further includes the step of adding at least one of zinc oxide or titanium dioxide to the aqueous solution of pure silk fibroin-based protein fragments.

According to aspects illustrated herein, a method is disclosed for producing silk gels having entrapped molecules or therapeutic agents such as those listed in the following paragraphs.

In an embodiment, at least one molecule or therapeutic agent of interest is physically entrapped into a SPF mixture solution of the present disclosure during processing into aqueous gels.

An aqueous silk gel of the present disclosure can be used to release atleast one molecule or therapeutic agent of interest.

According to aspects illustrated herein, pure silk fibroin-based protein fragments from aqueous solutions of the present disclosure can be formed into yams and fabrics includingfor example, woven or weaved fabrics, and these fabrics can be used in textiles, as described above.

According to aspects illustrated herein, silk fabric manufactured from SPF mixture solutions of the present disclosure are disclosed.

In an embodiment, at least one molecule or therapeutic agent of interest is physically entrapped into a SPF mixture solution of tide present disclosure. A silk film of the present disclosure can be used to release atleast one molecule or therapeutic agent of interest.

In some embodiments, the invention may include an article having a fiber or yam having a coating, wherein the coating may include silk based proteins or fragments thereof hawing a weight average molecular weight range of about 5 kDa to about 144 kDa.

In some embodiments, the article may be a fabric.

In some embodiments, the silk based proteins or fragments thereof may include silk fibroin-based proteins or protein fragments having about 0,01% (w/w) to about 10% (w/w) sericin.

In some embodiments, the silk based proteins or fragments thereof may be selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof.

In some embodiments, the silk based proteins or fragments thereof may be natural silk based proteins or fragments thereof that may be selected from the group consisting of 275 Date re?ue/Date received 2023-07-20 spider silk based proteins or fragments thereof, silkworm silk based proteins orfragments thereof, and combinations thereof.

In some embodiments, the natural silk based proteins or fragments may be silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof may be Bambyx mori silk based proteins or fragments thereof.

In some embodiments, the silk based proteins or fragments may include silk and a copolymer.

In some embodiments, the silk based proteins or protein fragments thereof may have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof may have a polydispersity of between about 1.5 and about 3.0, and wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for at least 10 days.

In some embodiments, the fiber or yam may be selected from the group consisting of natural fiber or yarn, synthetic fiber oryam, or combinations thereof.

In some embodiments, the fiber or yam may be natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof.

In some embodiments, the fiber or yam may be synthetic fiber or yam selected from the group consisting of polyester, nylon, polyester-polyurethane copolymer, and combinations thereof, In some embodiments, the fabric may exhibit an improved property, wherein the improved property may be an accumulative one-way moisture transport index selected from the group consisting of greater than 40 %, greater than 60 %, greater than 80 %, greater than 100 %, greater titan 120 %, greater than 140 %, greater than 160 %, and greater than 180%. 285 Date re?ue/Date received 2023-07-20 In some embodiments, the fabric may exhibit an improvedproperty, wherein the improvedproperty may be an accumulative one way transport capability increase relative to uncoated fabric selected from the group consisting of 1.2 fold, 1.5 fold, 2.0 fold, 3.0 fold, 4.0 fold, 5.0 fold, and 10 fold.

In some embodiments, the fabric may exhibit an improved property, wherein the improved property may be an overall moisture management capability selected from the group consisting of greater than 0.05, greater than 0.10, greater than 0.15, greater than 0.20, greater than0.25, greater than 0.30, greatarthan 0.35, greater than 0.40, greater than 0.50, greater than 0.60, greater than 0.70, and greater than 0.80.

In some embodiments, the improved property may be determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In some embodiments, the fabric may exhibit substantially no increase in microbial growth after a number of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In some embodiments, the microbial growth may be microbial growth of a microbe selected from die group consisting of Staphylococcus aureus, Klebisiella pneumoniae, and combinations thereof.

In some embodiments, the microbial growth may be reduced by a percentage selected from the group consisting of 50 %, 100 %, 500 %, 1000 %, 2000 %, and 3000 % comparedto anuncoated fabric.

In some embodiments, the coating may be applied to the fabric at the fiber level prior to forming the fabric.

Insome embodiments,the coatingmaybe applied tothe fabric at the fabric level.

In sone embodiments, the fabric may bebath coated.

In some embodiments, the fabric may be spray coated, in some embodiments, the fabric may be coated with a stencil.

In some embodiments, the coating may be applied to at least one side of the fabric using a method selected from the group consisting of a bath coating process, a spray coating process, astencilprocess, a silk-foam based process, and aroller-based process.

Insome embodiments,the coatingmayhave athickness ofabout one nanolayer. 295 Date re?ue/Date received 2023-07-20 In some embodiments, the coating may have a thickness selected from the group consisting of about 5 nm, about 10 nm, about 15 nm, about 20 nm, about 25 nm, about 50 nm, about 100 tun, about 200 nm, about 500 nm, about 1 pm, about 5 tun, about 10 gm, and about 20 um.

In some embodiments, the coating may be adsorbed on the fabric.

In some embodiments, the coaling may be attached to the fabric through chemical, enzymatic, thermal, or inradiative cross-linking.

In some embodiments, the hand of the coated fabric may be improved relative to an uncoated fabric.

In some embodiments, the hand of the coated fabric that may be improved may be selected from the group consisting of softness, crispness, dryness, silkiness, and combinations thereof.

In some embodiments, a flame retardation property of the coated fabric may be improved relativeto an uncoated fabric.

In some embodiments, a flame retardation property of an uncoated fabric may not be adversely affected by the coating.

In some embodiments, the abrasion resistance may be improved relative to an uncoated fabric.

In an embodiment, the invention may include an article comprising a textile or leather having a coating, whorein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa.

In some embodiments, the silk based proteins or protein fragments thereof have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and wherein the proteins or protein fragments, prior to 305 Date re$ue/Date received 2023-07-20 coating the fabric, do not spontaneously or gradually gdate and do not visibly change in color or turbidity whenin a solution for atleast 10 days.

In some embodiments, at least one property of the article may be improved, wherein dieproperty that may be improved may be selected from the group consisting of color retention, resistance to microbial growth, resistance to bacterial growth, resistance to fungal growth, resistance to the buildup of static electrical charge, resistance to the growth ofmildew, transparency of the coating, resistance to freeze-thaw cycle damage, resistance from abrasion, blocking of ultraviolet (UV) radiation, regulation of the body temperature of a wearer, resistance to tearing, elasticity of die article, rebound dampening, tendency to cause itching in die wearer, thermal insulation of the wearer, wrinkle resistance, stainresistance, stickiness to skin, and flame resistance.

Insomeembodiments,the articlemay bea textileusedfor apparel.

Insome embodiments, the article may be fabricated as anitem selected from the group consisting of an item of athletic apparel, an item of outdoor gear, a jacket, an overcoat, a shoe, a sneaker, a glove, an umbrella, a chair, a blanket, a towel, a surgical drape, a surgical gown, a laboratory coat, a wound dressing, a sterilization wrap, a surgical face mat, a surgical sleeve, a laboratory sleeve, a retention bandage, a support device, a compressionbandage, a shoe cover, and a surgicalblanket.

In some embodiments, the article may be a textile, leather, or foam used to fabricateanautomotiveproduct.

Insome embodiments, the article may be fabricated as anitem selected from the group consist!ngof anupholstery, a foam cushion, a fabric cushion, a floor mat,a vehicle carpet, an automotive trim, a children’s car seat, a seat belt, a safety harness, a headrest, an armrest, a dashboard, a sunvisor, a seat, aninterior panel, an airbag, an airbag cover, a wiringharness, or aninsulation.

In an embodiment, the invention may include a method of coating a fabric that may include the step of optionally applying a pretreatment selected from 1he group consistingof a wetting agent, a detergent, a sequestering or dispersing agent, an enzyme, a bleaching agent, an antifoaming agent, an anti-creasing agent, a dye dispersing agent, a 315 Date re?ue/Date received 2023-07-20 dye leveling agent, a dye fixing agent, a dye special resin agent, a dye anti-reducing agent, a pigment dye system anti-migrating agent, a pigment dye system binder, a delave agent, a wrinkle free treatment, a softener, a handle modifier, a waterborne polyurethane dispersion, a finishing resin, an oil or water repellant, a flame retardant, a crosslinker, a thickener fat technical finishing, or any combination thereof.

In an embodiment, the method may include the step of applying a coating that may include a solution of silk based proteins or fragments thereof that may have an average molecular weight range of about 5 kDa to about 144 kDa, using a process selected from the group consisting of a continuous spray process, a continuous screen or stencil process, a continuous bath process, a batch spray process, a batch screen or stencil process, and a batch bath process.

In an embodiment, the method may include the step of drying and optionally curing the coating.

In an embodiment, the silk based proteins or protein fragments thereof may have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, abort 17 kDa to about 38 kDa. about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof may have a polydispersity of between about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for at least 10 days.

BRIEF DESCRIPTION OF THE DRAWINGS The presently disclosed embodiments will be further explained with reference to the attached drawings.

The drawings shown are not necessarily to scale, with emphasis instead generally being placed upon illustrating the principles of the presently disclosed embodiments.

Fig, 1 is a flow chart showing various embodiments for producing pure silk fibroin-based protein fragments (SPFs) of the present disclosure. 325 Date re?ue/Date received 2023-07-20 Fig. 2 is a flow chart showing various parameters that canbe modified during the process of producing SPFs of the present disclosure during the extraction and the dissolution steps.

Fig. 3 is aphotograph showing dry extracted silk fibroin.

Fig. 4 is aphotograph showing an embodiment of a SPF inthe form ofa solution of the present disclosure.

Figs. 5A-5D are photographs showing dissolved silkin room temperature lithium bromide (LiBr) solutions dissolved in a 60°C oven for 4 hours (sericin extraction temperature andtime were varied).

Figs. 6A-6D are photographs showing dissolved silk in room temperature LiBr solutions dissolved in a 60°C oven for 6 hours (sericin extraction temperature and time werevaried).

Figs. 7A-7D are photographs showing dissolved silk in room temperature LiBr solutions dissolved in a 60°C oven for 8 hours (sericin extraction temperature and time werevaried).

Figs. 8A-8D are photographs showing dissolved silk in room temperature LiBr solutions dissolved in a 60°C oven for 12 hours (sericin extraction temperature and time werevaried).

Figs. 9A-9D are photographs showing dissolved silk in room temperature LiBr solutions dissolved in a 60°C oven for 24 hours (sericin extraction temperature and time werevaried).

F^gs. 10A-10C are photographs showing dissolved silk inroom temperatureLiBr solutions dissolved in a 60°C oven for 168/192 hours(sericin extraction temperature and time werevaried).

Figs. 11A-11C are photographs showing dissolved silk in room temperatureLiBr solutions dissolved in 60°C oven for 1, 4, and 6 hairs, where sericin extraction was completed at 100°C for 60 min. 335 Date re?ue/Date received 2023-07-20 Figt 12A-12D are photographs showing dissolved silk in 60°C LiBr solutions dissolved in a 60°C oven for 1 hour (sericin extraction temperature and time were varied).

Figs. 13A-13D are photographs showing dissolved silk in 60°C LiBr solutions dissolved in a 60°C oven for 4 hours (sericin extraction temperature and time were varied).

Figs. 14A-14D are photographs showing dissolved silk in 60°C LiBr solutions dissolved in a 60’C oven for 6 hours (sericin extraction temperature and time were varied).

Figs. 15A-15D are photographs showing dissolved silk in 80°C LiBr solutions dissolved in a 60°C oven for 1 hour (sericin extraction temperature and time were varied).

Figs. 16A-16D are photographs showing dissolved silk in 80°C LiBr solutions dissolved in a 60°C oven for 4 hours (sericin extraction temperature and time were varied).

FigS. 17A-17D are photographs showing dissolved silk in 80°C LiBr solutions dissolved in a 60°C oven for 4 hours (sericin extraction temperature and time were varied).

Figs. 18A-18D are photographs showing dissolved silk in 100°C LiBr solutions dissolved in a 60°C oven for 1 hour (sericin extraction temperature and time were varied).

Figs. 19A-19D are photographs showing dissolved silk in 100°C LiBr solutions dissolved in a 60°C oven for 4 hours (sericin extraction temperature and time were varied).

Figs. 20A-20D are photographs showing dissolved silk in 100°C LiBr solutions dissolved in a 60°C oven for 6 hours (sericin extraction temperature and time were varied). 345 Date re?ue/Date received 2023-07-20 Figs. 21A-21D are photographs showing dissolved silk in 140°C (boiling point for LiBr) LiBr solutions dissolved in a 60°C oven for 1 hour (sericin extraction temperature andtime were varied time).

Figs. 22A-22® are photographs showing dissolved silk in 140°C (boiling point for LiBr) LiBr solutions dissolved in a 60°C oven for 4 hours (sericin extraction temperatureandtime were varied).

Figs. 23A-23D are photographs showing dissolved silk in 140°C (boiling point for LiBr) LiBr solutions dissolved in a 60°C oven for 6 hours (sericin extraction temperatureandtime were varied).

F^ 24A-24D are photographs showing dissolved silk in 80°C LiBr solutions dissolved in a 80°C oven for 1 hour (sericin extraction temperature and time were varied).

Figs. 25A-25D are photographs showing dissolved silk in 80°C LiBr solutions dissolved in a 80°C oven for 4 hours (sericin extraction temperature and time were varied).

F^s. 26A-26D are photographs showing dissolved silk in 80°C LiBr solutions dissolved in a 80°C oven for 6 hours (sericin extraction temperature and time were varied).

Figs. 27A-27D are photographs showing dissolved silk in 100°C LiBr solutions dissolved in a 100°C oven for 1 hour (sericin extraction temperature and time were varied).

Figs. 28A-28D are photographs showing dissolved silk in 100°C LiBr solutions dissolved in a 100°C oven for 4 hours (sericin extraction temperature and time were varied).

Figs. 29A-29D are photographs showing dissolved silk in 100°C LiBr solutions dissolved in a 100°C oven for 6 hours (sericin extraction temperature and time were varied). 355 Date re?ue/Date received 2023-07-20 F^p. 30A-30D are photographs showing dissolved silk in 140°C (boiling point for LiBr) LiBr solutions dissolved in a 120°C oven for 1 hour (sericin extraction temperature andtime were varied).

Figs. 31A-31D are photographs showing dissolved silk in 140°C (boiling point for LiBr) LiBr solutions dissolved in a 120°C oven for 4 hours (sericin extraction temperatureandtime were varied).

Fig. 32A-32D are photographs showing dissolved silk in 140°C (boilingpoint for LiBr) LiBr solutions dissolved in a 120°C oven for 6 hours (sericin extraction temperatureandtime were varied).

F^ 33 shows HPLC chromatograms from samples comprising vitamin C.

Fig. 33 shows peaks from (1) a chemically stabilized sample of vitamin C at ambient conditions and (2) a sampleofvitamin C takenafter 1hour at ambient conditions without chemical stabilizationtoprevent oxidation, where degradation products are visible.

Fig. 34 is a table summarizing the LiBr and Sodium Carbonate (Na^Oj) concentration insilk protein solutions ofthe present disclosure.

Fig. 35 is a table summarizing theLiBr and NaiCOj concentration in silk protein solutionsofthe present disclosure.

Fig. 36 is a table summarizing the stability of vitamin C in chemically stabilized solutions.

Fig. 37 is a table summarizing theMolecular Weights of silk protein solutions of the present disclosure.

Figs. 38A and 38B are graphs representing the effect of extraction volume on % mass loss.

Fig. 39 is a table summarizing the Molecular Weights of silk dissolved from different concentrations ofLiBr and from difFerent extraction and dissolution sizes.

Fig. 40 is a graph summarizing the effect of Extraction Time on Molecular Wright of silk processed under the conditions of 100°C Extraction Temperature, 100°C LiBr and 100°C OvenDissolution(Oven/Dissolution Time was varied). 365 Date re?ue/Date received 2023-07-20 Fig. 41 is a graph summarizing the effect of Extraction Time on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, boiling LiBr and 60°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 42 is a graph summarizing the effect of Extraction Time on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 60°C LiBr and 60°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 43 is a graph summarizing the effect of Extraction Time on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 80°C LiBr and MFC Oven Dissolution (Oven/Dissolution Time was varied). 44 is a graph summarizing the effect of Extraction Time on Molecular Weight of silk processed under the corufitions of 10(FC Extraction Temperature, 80°C LiBr and 60°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 45 is a graph summarizing the effect of Extraction Time on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 100°C LiBr and 60°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 46 is a graph summarizing the effect of Extraction Time on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 140°C LiBr and 140°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 47 isa graph summarizing theeffect of Extraction Temperature on Molecular Weight of silk processed under the conditions of 60 minute Extraction Time, 100°C LiBr and 100°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 48 is a graph summarizing the effect of LiBr Temperature on Molecular Weight of silk processed under the conditions of 60 minute Extraction Time, 100°C Extraction Temperature and 60°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 49 is a graph summarizing the effect of LiBr Temperature on Molecular Weight of silk processed under the conditions of 30 minute Extraction Time, 100°C Extraction Temperature and 60°C Oven Dissolution (Oven/Dissolution Time was varied).

Fig. 50 is a graph summarizing tire effect of Oven/Dissolution Temperature on Molecular Weight of silk processed under the conditions of 100°C Extraction 375 re?ae?Bate received 2&23-V7-2Q Temperature, 30 minute Extraction Time, and 100°C Lithium Bromide (Oven/Dissolution Time was varied).

Fig, 51 is a graph summarizing die effect of Oven/Dissolution Temperature on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 60 minute Extraction Time, and 100°C Lithium Bromide. (Oven/Dissolution Time was varied).

Fig. 52 is a graph summarizing die effect of Oven/Dissolution Temperature on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 60 minute Extraction Time, and 140°C Lithium Bromide (Oven/Dissolution Time was varied).

Fig. 53 is a graph summarizing die effect of Oven/Dissolution Temperature on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 30 minute Extraction Time, and 140°C Lithium Bromide (Oven/Dissolution Time was varied).

Fig. 54 is a graph summarizing die effect of Oven/Dissolution Temperature on Molecular Weight of silk processed under the conditions of 100°C Extraction Temperature, 60 minute Extraction Time, and 80°C Lithium Bromide (Oven/Dissolution Time was varied).

Fig. 55 is a graph summarizing die Molecular Weights of silk processed under varying conditions including Extraction Time, Extraction Temperature, Lithium Bromide (LiBr) Temperature, Oven Temperature for Dissolution, Oven Timefor Dissolution.

Fig. 56 is a gfaph summarizing die Molecular Weights of silk processed under conditionsin which Oven/Dissolution Temperature is equal to LiBr Temperature.

Fig. 57A is a graph illustrating wetting time with spray coating.

Fig.57Bis a graph illustrating wetting time with stencil coating.

Fig.57C is a graph illustrating wetting time with bath coating.

Fig.57D is a graph illustrating wetting time with screen coating.

F^.58A is a graph illustrating absorption time with spray coating. 385 Date re?ue/Date received 2023-07-20 Fig. 58B is a seraph illustrating absorption time with stencil coating.

Fig. 58Cis a graph illustrating absorption time with bath coating.

Fig. 58D is a graph illustrating absorption time with screen coating.

Fig. 59A is a graph illustrating spreading speed with spray coating.

Fig. 59Bis a graph illustrating spreading speed with stencil coating.

Fig. 59C is a graph illustrating spreading speed with bath coating.

Fig. 59D is a graph illustrating spreading speed with screen coating.

Fig. 60A is a graph illustrating accumulative one way transport index with spray coating.

Fig. 60Bis a graph illustrating accumulative one way transport index with stencil coating.

Fig. 60Cis a graph illustrating accumulative one way transport index with bath coating.

F^. 60D is a graph illustrating accumulative one way transport index with screen coating.

Fig. 61A is a graph illustrating overall moisture management capability with spray coating.

Fig. 6IB is a graph illustrating overall moisture management capability with stencil coating.

Fig. 61C is a graph illustrating overall moisture management capability with bath coating.

Fig. 6ID is a graph illustrating overall moisture management capability with screen coating.

Fig. 62A is a graph illustrating wetting time top.

Fig. 62Bis a graph illustrating wetting time bottom.

Fig. 63A is a graph illustrating top absorption rate. 395 Date re?ue/Date received 2023-07-20 Fig. 63Bis a seraph illustrating bottom absorption rate.

Fig. 64A is a graph illustrating top max wetted radius.

Fig. 64Bis a graph illustrating bottom max wetted radius.

Fig. 65A is a graph illustrating top spreading speed.

Fig. 65Bis a graph illustrating bottom spreading speed.

Fig. 66A is a graph illustrating accumulative one-way transport index.

Fig. 66B is a graph illustrating overall moisture management capability.

Kg.67A is a graph illustrating wetting time of non-wickingfinished.

Fig. 67B is a graph illustrating wetting time of semi-finished before final setting.

Fig. 68A is a graph illustrating absorption time of non-wicking finished.

Fig. 68Bis a graph illustrating absorption time of semi-finished before final setting Fig. 69A is a graph illustrating spreading speed of non-wicking finished.

Fig. 69Bis a graph illustrating spreading speed of semi-finished before final setting.

Fig. 70A is a graph illustrating accumulative one way transport index of nonwieking finished.

Fig, 70Bis a graph illustrating accumulative one way transport index of semi¬ finished before final setting.

Fig. 71A is a graph illustrating overall moisture management capability of nonwicking finished.

F^. 71B is a graph illustrating overall moisture management capability of semi¬ finished before final setting.

F®. 72A is a graph illustrating wetting time with spray coating.

Fig. 72Bis a graph illustrating wetting time with stencil coating.

Fig. 72Cis a graph illustrating wetting time with bath coating. 405 Date re?ue/Date received 2023-07-20 Fig. 73A is a graph illustrating absorption time with spray coating.

Fig. 73Bis a graph illustrating absorption time with stencil coating.

Fig. 73Cis a graph illustrating absorption time with bath coating.

Fig. 74A is a graph illustrating spreading speed with spray coating.

Fig. 74Bis a graph illustrating spreading speed with stencil coating.

Fig. 74C is a graph illustrating spreading speed with bath coating.

Fig. 75A is a graph illustrating accumulative one way transport index with spray coating.

F^.7SBis a graph illustrating accumulative one way transport index with stencil coating.

Fig. 75C is a graph illustrating accumulative one way transport index with bath coating, Fig. 76A is a graph illustrating overall moisture management capability with spray coating.

Fig. 76Bis a graph illustrating overall moisture management capability with stencil coating.

Fig. 76Cis a graph illustrating overall moisture management capability with bath coating.

Fig, 77A is a graph illustrating wetting time with 1% SFS.

F®. 77Bis a graph illustrating wetting time with 0.1% SFS.

F^ 78A is a graph illustrating absorption timewith 1% SFS.

Fig. 78B is a graph illustrating absorption time with 0.1% SFS.

Fig. 79A is a graph illustrating spreading speed with 1% SFS.

F®.79B is a graph illustrating spreading speed with 0.1% SFS.

Fig. 80A is a graph illustrating accumulative one way transport index with 1% SFS. 415 Date re?ue/Date received 2023-07-20 Fig.SOBis a gaph illustrating accumulative one way transport index with 0.1% SFS.

Fig. 81A is a graph illustrating overall moisturemanagement capability with 1% SFS.

Fig. 8IBis a graph illustrating overall moisturemanagement capability with 0.1% SFS.

Fig. 82Ais a graph illustrating summary of wetting time top.

Fig.82Bis a graph illustrating summary of wettingtime bottom.

F^ 83Ais a graph illustrating summary of top absorptionrate.

Fig. 83Bis a graph illustrating summary of bottom absorption rate.

F^.84A is a graph illustrating summary of top max wettedradius.

Fig.84Bis a graph illustrating summary ofbottom wettedradius.

Fig. 85A is a graph illustrating summary of top spreading speed.

Ffe.85Bis a graph illustrating summary of bottom spreading speed.

Fig.86A is a graph illustrating summary of accumulative one-way transport index.

Fig. 86Bis a graph illustrating summary of overall moisturemanagement capability.

F^.87 illustrates bacterial growthresults.

F^88 illustrates bacterial growthresults.

Fig. 89 illustrates bacterial growthresults.

F®.90 illustrates bacterial growthresults.

Fig. 91illustrates bacterial growthresults.

F®.92 illustrates bacterial growthresults.

Fig. 93 illustrates accumulative one-way transport index versus fabric washing cycles. 425 Date re?ue/Date received 2023-07-20 Fig.94 illustrates overall moisture management capability (OMMC) versus fabric washing cycles.

Fig.95 illustrates wetting time at the top of thefabric versusfabric washing cycles.

Fig.96 illustrates wetting time at the bottom of the fabric versus fabric washing cycles.

Fig. 97 illustrates absorption rate at the top of the fabric versus fabric washing cycles.

Fig.98 illustrates absorption rate at the bottom of thefabricversus fabric washing cycles.

Fig.99 illustrates spreading speed at thetop of the fabric versus fabric washing cycles.

Fig.100 illustrates spreading speed at the bottom of tire fabric versus fabric washing cycles.

Fig.101illustrates wetted radius atthe top of the fabric versus fabric washing cycles.

Fig.102 illustrates wetted radius at the bottom of thefabric versus fabric washing cycles.

Fig.103illustrates percent reduction in growth of Staphylococcusaureus ATCC 6538versus fabric washing cycles.

Fig.104 illustrates percent reduction in growth of Klebisiella pneumoniae ATCC 4354 versus fabric washing cycles.

Fig.105illustrates a scanning electron microscopy image of fabric sample FAB- 01-BATH-B (first view).

Fig.106 illustrates a scanning electron microscopy image of fabric sample FAB- 01-BATH-B (second view). 435 Date re?ue/Date received 2023-07-20 Fig.107 illustrates a scanning electron microscopy image of fabric sample FAB- 01-BATH-B (third view).

Fig.108 illustrates a scanning electron microscopy image of fabric sample FAB- 01-BATH-B (fourth view).

Fig.109 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (first view).

Fig.110 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (second view).

Fig.

Ill illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (third view).

F^.112 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (fourth view).

Fig.113illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (fifth view).

Fig.114 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (sixth view).

Fig.1ISillustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-B (seventh view).

Fig.116 illustrates a scanning electron microscopy image offabric sample FAB- 01-SPRAY-C (first view).

Fig.117 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-C (second view).

Fig.118illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-C (third view).

Fig.119 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-C (fourth view). 445 Date re?ue/Date received 2023-07-20 Fig.120 illustrates a scanning electron microscopy image of fabric sample FAB- 01-SPRAY-C (fifth view).

Fig.121 illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (first view).

Fig.122 illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (second view).

Fig. 123illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (thin!view).

Fig.124 illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (fourth view).

F®.12S illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (fifth view).

Fig.126 illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (sixth view).

Fig.127 illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (seventh view).

Fig.128illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (eighth view).

Fig.129 illustrates a scanning electron microscopy image of fabric sample FAB- 01-STEN-C (ninth view).

Fig.130 illustrates a scanning electron microscopy image offabric sample FAB- 10-BATH-B (first view).

Fig.131 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-B (second view).

Fig.132 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-B (third view). 455 Date re?ue/Date received 2023-07-20 Fig.133illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-B (fourth view).

Fig.134 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-B (fifth view).

Fig.135illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-B (sixth view).

Fig. 136 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-B (seventh view).

Fig.137 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (first view).

Fig.138 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (second view).

Fig.139 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (third view).

Fig. 140 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (fourth view).

Fig.141illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (fifth view).

Fig.142 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (sixth view).

Fig. 143 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (seventh view).

Fig.144 illustrates a scanning election microscopy image of fabric sample FAB- 10-BATH-C (eighth view).

Fig.145 illustrates a scanning electron microscopy image of fabric sample FAB- 10-BATH-C (ninth view). 465 Date re?ue/Date received 2023-07-20 Fig.146 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (first view).

Fig.147 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (second view).

Fig. 148 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (third view).

Fig.149 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (fourth view).

Fig.150 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (fifth view).

Fig.151illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (sixth view).

Fig.152 illustrates a scanning electron microscopy image of fabric sampleFAB- 10-SPRAY-B (seventh view).

Fig.153illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (eighth view).

Fig.154 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-B (mirth view).

Fig.155 illustrates a scanning electron microscopy image of fabric sample FAB- 10-SPRAY-C.

Fig.156 illustrates a scanning electron microscopy image of fabric sample FAB- 10-STEN-B (first view).

Fig.157 illustrates a scanning electron microscopy image of fabric sample FAB- 10-STEN-B (second view).

Fig. 158 illustrates a scanning electron microscopy image offabric sample FAB- 10-STEN-B (third view). 475 Date re?ue/Date received 2023-07-20 Fig.159 illustrates a scanning electron microscopy image of fabric sample FAB- 10-STENB (fourth view).

Fig.160 illustrates a scanning electron microscopy image offabric sample FAB- 10-STEN-B (fifth view).

Fig.161 illustrates a scanning electron microscopy image of fabric sample FAB- 10-STEN-B (sixth view).

Fig. 162 illustrates a scanning electron microscopy image of fabric sample FAB- 10-STEN-B (seventh view).

Fig.163illustrates a scanning electron microscopy image of fabric sample FAB- 10-STEN-B (eighth view).

Fig. 164 illustrates a scanning electron microscopy image of a fabric control sample (first view).

F^165 illustrates a scanning electron microscopy image of a fabric control sample (second view).

Fig.166 illustrates a scanning electron microscopy image of a fabric control sample (third view).

Fig.167 illustrates a scanning electron microscopy image of a fabric control sample (fourth view). 168 illustrates a scanning electron microscopy image of film sample FIL-01- BATH-B-01MYL (first view).

Fig.169 illustrates a scanning electron microscopy image of film sampleFIL-01- BATH-B-01MYL (second view).

Fig.170 illustrates a scanning electron microscopy image of film sample FIL-01- BATH-B-01MYL (third view).

Fig. 171 illustrates a scanning electron microscopy image of film sample F1L-01- BATH-B-01MYL (fourth view). 485 Date re?ue/Date received 2023-07-20 Fig. 172 illustrates a scanning electronmicroscopy image of film sampleFIL-01- BATH-B-01MYL (Fifth view).

Fig.173 illustrates a scanning electron microscopy image of film sampleFIL-01- BATH-B-01MYL (sixthview).

Fig. 174 illustrates a scanning electronmicroscopy image of film sampleFIL-01- BATH-B-01MYL (seventhview).

Fig, 175 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-01MYL (first view).

Fig. 176 illustrates a scanning electronmicroscopy image of film sampleFIL-01- SPRAY-B-01MYL (second view).

F^.177 illustrates a scanning electronmicroscopy image of film sampleFIL-01- SPRAY-B-01MYL (third view).

Fig. 178 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-01MYL (fourth view).

Fig. 179 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-01MYL (fifthview).

Fig. 180 illustrates a scanning electronmicroscopy image of film sampleFIL-01- SPRAY-B-01MYL (sixth view).

Fig. 181illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-01MYL (seventhview).

Fig. 182 illustrates a scanning electronmicroscopy image of film sampleFIL-01- SPRAY-B-01MYL (eighth view).

Fig.183 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-007MYL (first view).

Fig.184 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O07MYL (secondview). 495 Date re?ue/Date received 2023-07-20 Fig.185 illustrates a scanning electron microscopy image of film sample FIL-01- SPRAY-B-O07MYL (third view).

Fig.186 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O07MYL (fourth view).

Fig. 187 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-007MYL (fifth view).

Fig. 188 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O1MYL cross-section(first view).

Fig. 189 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O1MYL cross-section (secondview).

F^.190 illustrates a scanning electronmicroscopy image of film sampleFIL-01- SPRAY-B-O1MYL cross-section(third view).

Fig.191 illustrates a scanning electron microscopy image of film sample FIL-01- SPRAY-B-O1MYL cross-section (fourth view).

W 192 illustrates a scanning electronmicroscopy image offilm sampleFIL-01- SPRAY-C-01MYL (first view).

Fig. 193 illustrates a scanning electronmicroscopy image of film sampleFIL-01- SPRAY-C-01MYL (secondview).

W 194 illustrates a scanning electron microscopy imageof film sampleFIL-01- SPRAY-C-01MYL (third view).

Fig. 19S illustrates a scanning electron microscopy image of film sampleF1L-01- SPRAY-C-01MYL (fourth view).

Fig.196 illustrates a scanning electron microscopy image of film sample FIL-01- SPRAY-C-01MYL (fifthview).

Fig.197 illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-B-01-MYL (first view). 505 Date re?ue/Date received 2023-07-20 Fig. 198 illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-B-01-MYL (second view).

Fig.199 illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-B-O1-MYL (thirdview).

Fig. 200 illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-B4H-MYL (fourth view).

Fig. 201illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-C-01-MYL (first view).

Fig. 202 illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-C-01-MYL (second view).

F^.203 illustrates a scanning electronmicroscopy image of film sampleFIL-01- STEN-C-01-MYL (third view).

Fig. 204 illustrates a scanning electron microscopy image of film sampleFIL-01- STEN-C-01-MYL (fourth view). w 205 illustrates a scanning electronmicroscopy image offilm sampleFIL-01- STEN-C-O1-MYL(fifthview).

Fig. 206 illustrates a scanning electronmicroscopy image of film sampleFIL-01- STEN-C-01-MYL (sixth view).

Fig. 207 illustrates a scanning electron microscopy image of film sampleFIL-10- BATH-B-01MYL (first view).

Fig. 208 illustrates a scanning electron microscopy image of film sampleF1L-10- BATH-B-01MYL (second view).

Fig. 209 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-01MYL (thirdview).

Fig. 210 illustrates a scanning electron microscopy image of film sampleFIL-10- BATH-B-01MYL (fourth view). 515 Date re?ue/Date received 2023-07-20 Fig. 211 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-01MYL (Fifth view).

Fig. 212 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-01MYL (sixth view).

Fig. 213 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-01MYL (seventh view).

Fig. 214 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-007MEL (first view).

Fig. 215 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-007MEL (second view).

F^. 216 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-007MEL (third view).

Fig. 217 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-007MEL (fourth view). w 218 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-B-007MEL (fifth view).

Fig. 219 illustrates a scanning electron microscopy image of film sample FIL-10- BATH-C-01MYL cross-section (first view).

Fig. 220 illustrates a scanning electron microscopy image of film sample FIL-10- SPRAY-B-01MYL (first view).

Fig. 221 illustrates a scanning electron microscopy image of film sample F1L-10- SPRAY-B-01MYL (second view).

Fig. 222 illustrates a scanning electron microscopy image of film sample FIL-10- SPRAY-B-01MYL (third view).

Fig. 223 illustrates a scanning electron microscopy image of film sample FIL-10- SPRAY-B-01MYL (fourth view). 525 Date regue/Date received 2023-07-20 Fig. 224 illustrates a scanning electron microscopy image of film sampleFIL-10- SPRAY-B-01MYL (fifthview).

Fig. 225 illustrates a scanning electron microscopy image of film sampleFIL-10- SPRAY-B-01MYL (sixth view).

Fig. 226 illustrates a scanning electron microscopy image of film sample FILBATH-C-01-MYL (firstview).

Fig, 227 illustrates a scanning electron microscopy image of film sample FILBATH-C-01-MYL (second view).

Fig. 228 illustrates a scanning electron microscopy image of film sample FILBATH-C-01-MYL (third view).

F^. 229 illustrates a scanning electron microscopy image of film sample FILBATH-C-01-MYL (fourth view).

Fig. 230 illustrates a scanning electron microscopy image of film sample FILBATH-C-01-MYL (fifthview).

Fig. 231 illustrates a scanning electron microscopy image of film sample FILBATH-C-01-MYL (sixth view).

Fig. 232 illustrates a scanning electronmicroscopy image of film sampleMelinex Control (first view).

Fig. 233 illustrates a scanning electronmicroscopy image of film sampleMelinex Control (secondview).

Fig. 234 illustrates a scanning electronmicroscopy image of film sampleMelinex Control (third view).

Fig.235 illustrates a scanning electron microscopy image of film sampleMelinex Control (fourth view).

Fig. 236 illustrates a scanning electron microscopy image of film sample Mylar Control (first view). 535 Date re?ue/Date received 2023-07-20 Fig. 237 illustrates a scanning electron microscopy image of film sample Mylar Control (second view).

Fig. 238 illustrates a scanning electron microscopy image of film sample Mylar Control (third view).

Fig. 239 illustrates a scanning electron microscopy image of film sample Mylar Control (fourth view).

Fig, 240 illustrates a scanning electron microscopy image of film sample Mylar Control (fifth view).

Fig. 241 shows results from optical profiling measurements on the Mylar Control sample taken at the top, location 1 (shiny side).

F^. 242 shows results from optical profiling measurements on the Mylar Control sample taken at the bottom, location 2 (more matte side).

Fig. 243 shows results from optical profiling measurements on the Melinex Control sample taken at the top, location 1.

Fig. 244 shows results from optical profiling measurements on the Melinex Control sample taken at the bottom, location 2.

Fig. 245 shows results from optical profiling measurements on sample FIL-10- SPRAY-B-01MYL taken at the top, location 1.

Fig. 246 shows results from optical profiling measurements on sample FIL-10- SPRAY-B-01MYL taken at the bottom, location 2.

Fig. 247 shows results from optical profiling measurements on sample FIL-01- SPRAY-B-01MYL taken at the top, location 1.

Fig. 248 shows results from optical profiling measurements on sample FIL-01- SPRAY-B-01MYL taken at the bottom, location 2.

Fig. 249 shows results from optical profiling measurements on sample FIL-01- SPRAY-B-O07MEL taken the top, location 1. 545 Date re?ue/Date received 2023-07-20 Fig. 250 shows results from optical profiling measurements on sample FIL-01- SPRAY-B-O07MEL taken at the bottom, location 2.

Fig, 251 shows results from optical profiling measurements on sample FIL-01- SPRAY-C-01MYL taken at the top, location 1.

Fig, 252 shows results from optical profiling measurements on sample FIL-01- SPRAY-C-01MYL taken at bottom, location 2 Fig, 253 shows results from optical profiling measurements on sample FIL-01- STEN-B-01MYL taken at thetop, location1.

F^, 254 shows results from optical profiling measurements on sample FIL-01- STEN-B-01MYL taken at the bottom, location 2.

Fig. 255 shows results from optical profiling measurements on sample FIL-01- STEN-C-01MYL taken at thetop, location 1.

Fig. 256 shows results from optical profiling measurements on sample FIL-01- STEN-C-01MYL taken at the bottom, location 2.

Fig. 257 shows results from optical profiling measurements on sample FIL-10- BATH-B-01MYL taken at thetop, location 1.

Fig. 258 shows results from optical profiling measurements on sample FIL-10- BATH-B-01MYL taken at the bottom, Location 2. w 259 shows results from optical profiling measurements on sample FIL-10- BATH-B-007MEL taken at the top, location 1, Fig. 260 shows results from optical profiling measurements on sample F1L-10- BATH-B-007MEL taken at the bottom, location 2.

Fig. 261 shows results from optical profiling measurements on sample FIL-10- BATH-C-01MYL taken at top, location 1.

Fig. 262 shows results from optical profiling measurements on sample FIL-10- BATH-C-01MYL taken at the bottom, location 2. 555 Date re?ue/Date received 2023-07-20 Fig. 263 shows results from optical profiling measurements on sample FIL-01- BATH-B-01MYL taken at thetop,location 1.

Fig, 264 shows results from optical profiling measurements on sample FIL-01- BATH-B-01MYL takenat thebottom, location 2.

Fig. 265 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O1MYL cross-section.

Fig, 266 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O1MYL cross-section.

Fig. 267 illustrates a scanning electron microscopy image of film sampleFIL-01- SPRAY-B-O1MYL cross-section.

Fig. 268 illustrates a scanning electron microscopy image of film sampleFIL-10- BATH-C-01MYL cross-section.

F^ 269 illustrates accumulative one-way transport index results fir natural fibers.

Fig.270illustrates overall moisturemanagement capability for natural fibers.

Fig. 271 illustrates flammability test results for a cotton interlock fabric with (16021103) and without (16021101) coating with 1% silk fibroin solution.

Fig. 272 illustrates flammability test results for a cotton interlock fabric with (16021103) andwithout (16021101) coatingwith 1% silk fibroin solution.

Fig. 273illustrates flammability test results for apolyester doubleknitfabric with (16021104) andwithout (16021102) coatingwith 1% silk fibroin solution.

Fig. 274illustrates flammability test results for a polyester doubleknit fabric with (16021104) andwithout (16021102) coating with 1% silk fibroin solution Fig. 275 illustrates abrasion test results for a cotton interlock fabric with (16021501) andwithout (16021101) coatingwith 1% silk fibroin solution.

Fig. 276 illustrates abrasion test results for a polyester double knit fabric with (16021502) andwithout (16021102) coating with 1% silk fibroin solution. 565 Bate re?ne/Bate received zOzS-Oz-zO Fig.277 illustrates a scanning electron microscopeimage ofsample 16041301.

Fig.278 illustrates a scanning electronmicroscope image of sample 16041301.

Fig.279illustrates a scanning electronmicroscopeimage ofsample 16041301.

Fig.280illustrates a scanning electron microscopeimage ofsample 16041301.

Fig.281illustrates a scanning electron microscope image ofsample 16041301.

Fig. 282illustrates a scanning electron microscope image ofsample 16041302.

Fig.283illustrates a scanning electron microscope image ofsample 16041302.

Fig.284illustrates a scanning electron microscopeimage of sample 16041302.

Fig.285illustrates a scanning electron microscopeimage of sample 16041302.

Fig.286illustrates a scanning electronmicroscope image ofsample 16041302.

Fig.287illustrates a scanning electron microscope image of sample 16041303.

Fig.288illustrates a scanning electronmicroscope image of sample 16041303.

Fig.289illustrates a scanning electron microscope image of sample 16041303.

Fig.290illustrates a scanning electron microscope image of sample 16041303.

Fig.291illustrates a scanning electron microscope image of sample 16041303.

Fig.292illustrates a scanning electronmicroscope image of sample 16041304.

Fig.293 illustrates a scanning electron microscope image of sample 16041304.

Fig.294illustrates a scanning electronmicroscope image of sample 16041304.

Fig.295 illustrates a scanning electron microscope image of sample 16041304.

Ffe.296 illustrates a scanning electronmicroscope image of sample 16041304.

Fig.297illustrates a scanning electronmicroscope image ofsample 16041305.

Fig,298illustrates a scanning electron microscope image ofsample 16041305.

Fig.299illustrates a scanning electronmicroscope image ofsample 16041305.

Fig,300illustrates a scanning electron microscope image of sample 16041305. 575 Date re?ue/Date received 2023-07-20 Fig.301illustrates a scanning electron microscopeimage ofsample 16041305.

Fig.302illustrates a scanning electronmicroscope image of sample 16041306.

Fig.303illustrates a scanning electron microscopeimage ofsample 16041306.

Fig.304illustrates a scanning electron microscopeimage ofsample 16041306.

Fig.305illustrates a scanning electron microscope image ofsample 16041306.

Fig.306 illustrates a scanning electron microscope image ofsample 16041306.

Fig.307illustrates a scanning electron microscope image ofsample 16040803.

Fig.308illustrates a scanning electron microscopeimage of sample 16040803.

Fig.309illustrates a scanning electron microscopeimage of sample 16040803.

F®.310illustrates a scanning electronmicroscope image ofsample 16040803.

Fig.311illustrates a scanning electron microscope Image of sample 16040803.

Fig.312 illustrates a scanning electronmicroscope image of sample 16040808.

Fig.313illustrates a scanning electron microscope image of sample 16040808.

Fig.314illustrates a scanning electron microscope image of sample 16040808.

Fig.315 illustrates a scanning electron microscope image of sample 16040808.

Fig.316illustrates a scanning electronmicroscope image of sample 16040808.

Fig.317 illustrates an exemplary padderroller.

Fig.318illustrates anexemplary kiss roller Fig.319 illustrates theprocess ofunrolling an exemplary fabric roller.

Fig.320illustrates a squareof sample fabric tobe coated.

Fig.321illustrates an exemplary stainless steelbath.

Fig,322illustrates apadderunit havingtwo rollers.

Fig.323illustrates a curingframe without fabric providedthereon.

Fig,324illustrates a curingframewith fabric provided thereon. 585 Date re?ue/Date received 2023-07-20 Fig. 325illustrates an exemplary curing oven.

Kg. 326 illustrates a cooling rack with a curing frame and fabric provided thereon.

Mg. 327 illustrates a table that provides testing results for wetting time, absorption rate, wetted radius, spreading speed, accumulative one-way transport, and overall moisture management capability (OMMC) for sample nos. 16040101, 16040102, 16040103,16040104, 16040105, and 16040106.

Kg. 328 illustrates testing results in grades for wetting time, absorption rate, wetted radius, spreading speed, accumulative one-way transport and OMMC for 16040101, 1604010% 16040103, 16040104, 16040105, and 16040106.

Mg. 329 illustrates testing results for wetting time, absorptionrate, wetted radius, spreading speed, accumulative one-way transport, and OMMC for 16040801, 16040802, 16040803, 16040804, 16040805, 16040806, 16040807, and 16040808. »8. 330 illustrates testing results in grades far wetting time, absorption rate, wetted radius, spreading speed, accumulative one-way transport, and OMMC for 16040801, 16040802, 16040803, 16040804, 16040805, 16040806, 16040807, and 16040808.

Mg. 331 illustrates testingresults for wetting time, absorption rate, wetted radius, spreading speed, accumulative one-way transport, and OMMC for 16041201, 16041202, 16041302, 16041303, 16041203, 16041204, 16041305, 16041306, 16041301, and 16041304.

Kg. 332 illustrates testing results in grades for wetting time, absorption rate, wetted radius, spreading speed, accumulative one-way transport, and OMMC for 16041201, 16041202, 16041302, 16041303, 16041203, 16041204, 16041305, 16041306, 16041301, and 16041304.

Fig. 333 illustrates testingresults far wetting time, absorptionrate, wetted radius, spreading speed, accumulative one-way transport, and OMMC for 16041301, 16041302, 16041303, 16041304, 16041305, 16041306, 16042001, 16040101, and 16040106. 595 Date re?ue/Date received 2023-07-20 fig. 334 illustrates testing results in grades far wetting time, absorption rate, wetted radius, spreading speed, accumulative one-way transport, and OMMC for 16041301, 16041302, 16041303, 16041304, 16041305, 16041306, 16042001, 16040101, and 16040106.

Fig. 335 illustrates amap ofLiquidMoisture Management Test results for various coatedfabricsdescribed herein Fig. 336 illustrates drapability coefficient testing results for various STS coated fabrics.

Fig. 337 illustrates drapability coefficient testing results for an SFS coated fabric after mechanical and steam finishing.

Fig. 338 illustrates theresults of asolutiondepletion calculation duringcoating.

Fig. 339illustrates samplesused inmoisture management testing.

Fig. 340illustrates theresults of moisturemanagement testing.

Fig. 341illustrates samplesused inmoisture management testing.

Fig. 342 illustrates theresults of moisturemanagement testing Fig. 343 illustrates samplesused inmoisture management testing Fig. 344illustrates theresults of moisturemanagement testing.

Fig. 345illustrates rampiesused inantimicrobial testing Fig. 346 illustrates theresults of antimicrobial testing.

Fig. 347 illustrates theresults of a water drop teston polyester/lycra knitted fabric treatedwith Ultratex CSP.

Fig. 348 illustrates the results of awater drop test on polyester/lycra knitted fabric treatedwithUltratex SI.

Fig. 349 represents atable that describes test variables for an antibacterial study.

Fig. 350 represents a table that describes the study intervals for an antibacterial study 603 Date re?ue/Date received 2023-07-20 fig. 351 represents a table that describes the additional fabric bacteria load after washing cycles for an antibacterial study.

For example, after 1 washing cycle the additional fabric will receive 4xl07 of bacteriaload. figs. 352A and 3S2B represent tables that describe parameters and results for an antibacterial study.

Jig, 353 illustrates an image of bacterial colonies for sample 16060901 after washing. fig. 354 illustrates an image of bacterial colonies for sample 16060902 after washing. fig. 355 illustrates an image of bacterial colonies for sample 16060903 after washing. fig. 356 illustrates an image of bacterial colonies for sample 16060904 after washing.

Fig. 357 illustratesanimageofbacterial colonies for a control.

Fig.358 illustratesanimage ofbacterial colonies for acontrol.

Figs. 359A to 359C illustrate a microscopic image of coated fabric sample 16060901 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification,before any wash cycles.

Figs. 360A to 36OC illustrate a microscopic image of coated fabric sample 16060902 at (A) 350X magnification, (B) 1050X magnification, and (C) 35O0X magnification,before any wash cycles figs. 361A to 361C illustrate a microscopic image of coated fabric sample 16060903 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification,before any wash cycles Figs. 362A to 362C illustrate a microscopic image of coated fabric sample 16060904 at (A) 350X magnification, (B) 10S0X magnification, and (C) 3500X magnification, before any wash cycles. 615 Date repue/Date received 2023-07-20 Figs. 363A to 363C illustrate a microscopic image of coated fabric sample 16060901 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification, after one wash cycle.

Figs. 364A to 364C illustrate a microscopic image of coated fabric sample 16060902 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification, after one wash cycle. figs. 365A to 36SC illustrate a microscopic image of coated fabric sample 16060903 at (A) 350X magnification, (B) 105OX magnification, and (C) 3500X magnification, after one wash cycle.

Figs. 366A to 3660 illustrate a microscopic image of coated fabric sample 16060904 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification, after one wash cycle.

Figs. 367A to 367C illustrate a microscopic image of coated fabric sample 16060901 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification, after ten wash cycles.

Figs. 368A to 368C illustrate a microscopic image of coated fabric sample 16060902 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification, after ten wash cycles.

Figs. 369A to 369C illustrate a microscopic image of coated fabric sample 16060903 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnification, afteften wash cycles.

Figs. 370A to 370C illustrate a microscopic image of coated fabric sample 16060904 at (A) 350X magnification, (B) 1050X magnification, and (C) 3500X magnificat!on, after ten wash cycles.

Fig. 371 provides a qualitative analysis of the bacterial was study by observing the % foreignmatter coverage area observedinFigs. 359A-359CtoFigs. 370A-370C.

Fig, STB illustrates the results ofa water drop testonpolyesterflycra'knjtted ftbric treated withUltratex CSP. 625 Date re?ue/Date received 2023-07-20 Fig. 373 illustratesthe results ofawater drop test on polyestei/lycraknittedfabric treatedwithUltratex SI.

Fig. 374 illustratesdie results ofa water drop test onpolyester/lycraknittedfabric treatedwithRODIwater or tap water.

While the above-identified drawings set forth presently disclosed embodiments, other embodiments are also contemplated, as noted in the discussion.

This disclosure presents illustrative embodimentsby way of representation and not limitation.

Numerous other modifications and embodiments canbe devised by those skilledin the art which fall withinthe scope and spirit of theprinciples ofdie presently disclosedembodiments.

DETAILEDDESCRIPTIONOF THE INVENTION Silk Broin-Based Barf Provided herein are methods for producing pure and highly scalable silk protein fragment (SPF) mixture solutions that may be used to coat at least aportion of textiles or may be formed into usable fibers for weaving into yam.

In some embodiments, SPF mixture solutions may also refer to silk fibroin solutions (SFS), and vice versa.

The solutions are generated from raw pure intact silk protein material and processed in order to remove any sericin and achieve the desired weight average molecular weight (MW) and polydisparsity of the fragment mixture.

Select method parameters may be altered to achieve distinct final silk protein fragment characteristics depending upon the intended use.

The resulting final fragment solution is pure silk protein fragments and water with PPM to non-detectable levels of process contaminants.

The concentration, size and polydispersity of silk protein fragments in the solution may farther be altered depending upon the desired use and performance requirements.

In an embodiment, the pure silk fibroin-basedprotein fragments in the solution are substantially devoid of sericin, have mi averageweight average molecular weight ranging from about 6 kDato about 16 kDa, and have a polydispersity ranging from about 1.5 and about 3.0.

In an embodiment, the pure silk fibroin-based protein fragments in the solution are substantially devoid of sericin, 635 Date re?ue/Date received 2023-07-20 have an average weight average molecular weight ranging from about 17 kDa toabout 38 kDa, and have a polydispersity ranging from about 1.5 and about 3.0.

In an embodiment, the pure silk fibroin-based protein fragments in the solution are substantially devoid of sericin, have an average w eight average molecular weight ranging from about 39 kDa to about 80 kDa, and have a polydispersity ranging from about 1.5 and about 3.0.

In an embodiment, the solutions may be used to generate articles, such as silk gels of varying gel and liquid consistencies by varying water contenl/concentration, or sold as a raw ingredient into the consumer market As used herein, the term “silk solution” may refer to solutionsof silk proteins, including solutions of silk fibroin-based protein fragments.

As used herein, “siIk based proteins or fragments thereof’ includes silk fibroinbased proteins or fragments thereof, natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof Natural silk based proteins or fragments thereof include spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, Silkworm based proteins or fragments thereof may include Bombyx mori silk based proteins or fragments thereof.

The SPF mixture solutions described herein may include silk based proteins or fragments thereof.

Moreover, SFS, as described herein, may be replaced with SPF mixture solutions.

As used herein, “low molecular weight” silk fibroin solutions may include those SFS solutionis that include silk fibroin-based protein fragments having a molecular weight in a range of about 5 kDa to 20 kDa.

In some embodiments, a target low molecular weight farcertain silk fibroin-based protein fragments may be about 11 kDa, As used herein, “medium molecular weight” silk fibroin solutions may indude those SFS solutions that include silk-fibroin based protein fragments having a molecular weight in a range of about 20 kDa to about 55 kDa.

In some embodiments, a target medium molecular weight for certain silk fibroin-based protein fragments may be about 40 kDa.

As used herein, “high molecular weight” silk fibroin solutions may indude those SFS solutions that include silk-fibroin based protein fragments having a molecular weight that is in a range of about 55 kDa to about 150 kDa.

In some embodiments, a target high 645 Date re?ue/Date received 2023-07-20 molecular weight for certain silk flbroin-based protein fragments may be about 100 kDa to about 145 kDa.

As used herein, the terms “substantially sericin free” or “substantially devoid of sericin” refer to silk fibers in which a majority of the sericin protein has been removed.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.01% (w/w) and about 10.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.01% (w/w) and about 9.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.01% (w/w) and about 8.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.01% (w/w) and about 7.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.01% (w/w) and about 6.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.01% (w/w) and about 5.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0% (w/w) and about 4.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.05% (w/w) and about 4.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.1% (w/w) and about 4.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 0.5% (w/w) and about 4.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 1.0% (w/w) and about 4.0% (w/w) sericin. hi an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 1.5% (w/w) and about 4.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 2.0% (w/w) and about 4,0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having between about 2.5% (w/w) and about 4.0% (w/w) sericin.

In an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having a sericin content between about 0.01% (w/w) and about 0.1 % (w/w).

In 655 Date re$ue/Date received 2023-07-20 an embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having a sericin content below about 0.1% (w/w).

Inan embodiment, silk fibroin that is substantially devoid of sericin refers to silk fibroin having a sericin content below about 0.05 % (w/w).

In an embodiment, when a silk source is added to a boiling (100°C) aqueous solution of sodium carbonate for a treatment time of between about 30 minutes to about 60minutes, adegummingloss ofabout 26 wt % to about 31wt.% is obtained.

As used herein, the term “substantially homogeneous” may refer to pure silk fibroin-based protein fragments that are distributed in a normal distribution about an identifiedmolecular weight As used herein, the term “substantially homogeneous” may referto an even distributionof additive, for example vitaminC, throughout a composition of thepresent disclosure.

Asusedherein, the term “substantially free ofinorganic residuals” means that the composition exhibits residuals of 0.1 % (w/w) or less.

In an embodiment, substantially free of inoiganic residuals refers to a composition that exhibits residuals of 0.05% (w/w) or less.

In an embodiment, substantially free of inorganic residuals refers to a composition that exhibits residuals of 0.01% (w/w) or less.

In an embodiment, the amount of inorganic residuals is between 0 ppm (“non-detectable” or “ND”) and 1000 ppm.In anembodiment, the amount of inorganic residuals isND to about 500 ppm.In an embodiment, embodiment, embodiment, embodiment, the amount of inorganic residuals the amount of inorganic residuals the amount of inorganic residuals the amount of inorganic residuals is ND to about is ND to about is ND to about is ND to about 400 ppm.

In an 300 ppm.

In an 200 ppm.

In an 100 ppm.

In an embodiment, the amount ofinorganic residualsisbetween 10ppmand 1000 ppm.

As used herein, the term “substantially free of organic residuals” means that the composition exhibits residuals of 0.1 % (w/w) or less.

In an embodiment, substantially free of organic residuals refers to a composition that exhibits residuals of 0.05%(w/w) or less.

In an embodiment, substantially free of organic residuals refers to a composition that exhibits residuals of 0.01% (w/w) or less.

In an embodiment, the amount of organic residuals is between 0 ppm (“non-detectable” or “ND”) and 1000 ppm.

In an embodiment, the amount of organic residuals is ND to about 500 ppm.

In an 665 Date re?ue/Date received 2023-07-20 embodiment, the amount of organic residuals is ND to about 400 ppm.

In an embodiment, the amount of organic residuals is ND to about 300 ppm.

In an embodiment, the amount of organic residuals is ND to about 200 ppm.

In an embodiment, the amount of organic residuals is ND to about 100 ppm.

In an embodiment, the amount of organic residuals isbetween 10 ppm and 1000 ppm.

Compositions of the present disclosure are “biocompatible” or otherwise exhibit “biocompatibility” meaning that the compositions are compatible withliving tissue or a living system by not being toxic, injurious, or physiologically reactive and not causing immunological rejection or an inflammatory response.

Such biocompatibility can be evidenced by participants topically applying compositions of the present disclosure on their skinfor an extended period oftime.

In an embodiment, the extendedperiod oftime is about 3 days, hi an embodiment, the extended period of time is about 7 days.

In an embodiment, the extended period of time is about 14 days.

In an embodiment, the extendedperiodof time is about 21 days.In an embodiment, the extended period of time is about 30 days.

In an embodiment, the extended period of time is selected from the group consisting of about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7months, about 8 months, about 9 months, about 10 months., about 11 months, about 12 months, and indefinitely.

For example, in some embodiments, the coatings described herein arebiocompatible coatings.

In some embodiments, compositions described herein, which may be biocompatible compositions (e.g., biocompatible coatings that include silk), may be evaluated and comply with International Standard ISO 10993-1, titled the “Biological evaluation of medical devices-Part 1: Evaluation and testing within a risk management process.” In some embodiments, compositions described herein, which may be biocompatible compositions, may be evaluated under ISO 106993-1 for one or more of cytotoxicity, sensitization, hemocompatibility, pyrogenicity, implantation, genotoxicity, carcinogenicity,reproductive and developmental toxicity, and degradation.

Insome embodiments,compositions andarticlesdescribedherein, andmethods of preparing the same, include silk coated fabrics and textiles wherein the silk coating is partially dissolved in the fabric or textile.

Die fabric or textile may be a polymeric 675 Bate re?ue/Date received 2023-07-20 material such as those described elsewhere herein.

The term “partially dissolved'' includes mixing to form a dispersion of, e.g., a portion of a polymeric fabric or textile with a portion of the silk based coating.

In some embodiments, the dispersion may be a solid suspension (/.<?., a dispersion comprising domains on the order of 10 nm) or a solid solution a molecular dispersion) of silk in the polymeric fabric or textile.

In some embodiments. the dispersion may be localized at the surface interface between the silk coating and the polymeric fabric or textile, and may have a depth of 1 nm, 2 nm, 5 nm, 10 nm, 25 nm, 50 nm, 75 nm, 100 nm, or greater titan 100 nm, depending on the method of preparation.

In some embodiments, the dispersion may be a layer sandwiched between the polymeric fabric or textile and the silk coating.

In some embodiments, the dispersion may be prepared by coating silk, including silk fibroin with the characteristics described herein, onto the polymeric fabric or textile, and then performing an additional process to form the dispersion, including heating at a temperature of 100 °C, 125 °C, 150 °C, 175 °C, 200 °C, 225 °C, or 250 °C for a time period selected from the group consisting of 1 minute, 2 minutes, 5 minutes, 10 minutes, 15 minutes, 20 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, 8 hours, 16 hours, or 24 hours.

In some embodiments, heating may be performed at or above the glass transition temperature (Tg) of silk and/or the polymeric fabric or textile, which may be assessed by methods known in the art.

In some embodiments, the dispersion may be formed by coating silk, including silk fibroin with the characteristics described herein, onto the polymeric fabric or textile, and then performing an additional process to impregnate the silk coating into the polymeric fabric or textile, including treatment with an organic solvent.

Methods for characterizing the properties of polymers dissolved in one another are well known in the art and include differential scanning calorimetry and surface analysis methods capable of depth profiling, including spectroscopic methods.

Compositions of the present disclosure are‘hypoallergenic” meaning thatthey are relatively unlikely to cause an allergic reaction.

Such hypoallergenicity can be evidenced by participants topically applying compositions of the present disclosure on their skin for an extended period of time.

In an embodiment, the extended period of time is about 3 days.

In an embodiment, the extended period of time is about 7 days.

In an embodiment, the extended period of time is about 14 days In an embodiment, the extended period of 685 Date re?ue/Date received 2023-07-20 time is about 21 days.

In an embodiment, the extended period of time is about 30 days.

In an embodiment, the extended period of time is selected from the group consisting of about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, about 12 months, and indefinitely.

In some embodiments, where aqueous solutions are used to prepare SPF compositions or SPF containing coatings, the aqueous solutions may be prepared with DI water or tsp water.

As used herein, “tap water” refers to potable water provided by public utilities and water of comparable quality, regardless of the source, without further refinement such as by reverse osmosis, distillation, and/or deionization.

Therefore, the use of “DI water,” “RODI water,” or “water,” as set forth herein, may be understood to be interchangeable with “tap water” according to the processes described herein without deleterious effects to such processes.

Textiles and LgafliersCgated with SilkIfein^^^ As used herein, the term “washable” and “exhibiting washability” means that a silk coated fabric of the present disclosure is capable of being washed without shrinking, fading, or the like.

As used herein, the term “textile” refers to a flexible woven or non-woven material consisting of a network of natural or artificial fibers often referred to as fabric, thread, or yam.

In an embodiment, textiles can be used to fabricate clothing, shoes and bags.

In an embodiment, textiles can be used to fabricate carpeting, upholstered furnishings, window shades, towels, and coverings for tables, beds, and other flat surfaces.

In an embodiment, textiles can be used to fabricateflags, backpacks, tents, nets, handkerchiefs, balloons, kites, sails, and parachutes.

As used herein, the term “leather” refers to natural leather and synthetic leather.

Natural leather includes chrome-tanned leather (e.g, tanned using chromium sulfate and other chromium salts), vegetable-tanned leather (e,^, tanned using tannins), aldehydetanned leather (also known as wet-white leather, e.g., tanned using glutaraldehyde or oxazolidine compounds), brain-tanned leather, formaldehyde-tanned leather, Chamois leather (e.g., tanned using cod oils), rose-tanned leather (e.g., tanned using rose otto mis), 695 Date re$ue/Date received 2023-07-20 s\ iithetic-tanned leather (e.g., tanned using aromatic polymers), alum-tanned leather, patent leather, Vachetta leather, nubuck leather, and rawhide leather.

Natural leather also includes s^lit leather, full-grain leather, top-grain leather, and corrected-grain leather, the properties and preparation of which are known to those of skill in the art.

Synthetic leather indudes poromeric imitation leathers (e.g., polyurethane cm polyester), vinyl and polyamidefelt fibers, polyurethane, polyvinyl chloride, polyethylene (PE), polypropylene (PP), vinyl acetate copolymer (EVA), polyamide, polyester, textile-polymer composite microfibers, corfan, koskin, leatherette, BIOTHANE®, BIRK1BUC®, BIRKOFLOR®, GLARING®, ECOLORICA®, KYDEX®, LORICA®, NAUGAHYDE®, REXINE®, VEGETAN®, FABRIKOID®, or combinations thereof.

As used herein, the term “hand” refers to the feel of a fabric, which may be further described as the feeling of softness, crispness, dryness, silkiness, and combinations thereof.

Fabric hand is also referred to as “drape.” A fabric with a hard hand is coarse, rough, and generally less comfortable for the wearer. A fabric with a soft hand is fluid and smooth, such as fine silk or wool, and generally more comfortable for the wearer.

Fabric hand can be determined by comparison to collections of fabric samples, or by use of methods such as 1he Kawabata Evaluation System (KES) or the Fabric Assurance by Simple Testing (FAST) methods.

Behera and Hari, Ind J.

Fibre & Textile Res.,1994, 19, 168-71.

Asused herein, the term “yam” refers toa single or multi-fiber construct As used herein, a “coating” refers to a material, or combination of materials, that form a substantially continuous layer or film on an exterior surface of a substrate, such as a textile.

In some embodiments, a portion of the coating may penetrate at least partially into fee substrate.

In some embodiments, fee coating may penetrate at least partially into the interstices of a substrate.

In some embodiments, fee coating may be infused into a surface of fee substrate such that fee application of the coating, or coating process, may include infusing (at fee melting temperature of the substrate) at least one coating component at least partially into a surface of the substrate. A coating may be applied to a substrate by one or more of fee processes described herein. 705 Date re?ue/Date received 2023-07-20 Inembodiments describedwhere the cartingmay be infusedinto a surface ofthe substrate, the coating may be codissolved in a surface of the substrate such that a component of the coating may be intermixed inthe surface of the substrate to a depth of at least about 1nm, or at least about 2 nm, or at least about 3 nm, or at least about 4 nm, or at least about 5 nm, or at least about 6 nm, or at least about 7 nm, or at least about 8 nm, or at least about 9 nm, or at least about 10 nm, or at least about 20 nm, or at least about 30nm, or at least about 40 nm, or at least about 50 nm, or at least about 60 nm, or at least about 70 nm, or at least about 80 nm, or at least about 90 nm, or at least about 100nm.

In some embodiments, the coatingmay be infusedinto a surface of the substrate where the substrate includes one or more polymers including, but not limited to, polyester, polyamide, polyaramid, polytetrafluorethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethyleneglycol, ultrahigh molecular weightpolyethylene,high-performance polyethylene, nylon, andLYCRA? Asused herein, the team “bath coating” encompasses coating a fabric in a batch, immersinga fabric in abath, and submerging a fabricin abath.

Concepts of bath coaling are set forth in U.S.

Patent No. 4,521,458.

As used herein, and unless mote specifically described, the term “drying” may refer to drying a coated material as described herein at a temperature greater than room temperature(i.e., 20 °C).

Inan embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile used for human apparel, including performance and/or athletic apparel.

In an embodiment, die invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, and wherein the textile or leather product exhibits improved moisture management properties and/or resistance to microbial growth.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product used for home upholstery.

In an embodiment, the 715 Date re?ue/Date received 2023-07-20 invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile or leather product is used for automobile upholstery.

In an embodiment, the invention provides a textile orleather product coated with silk fibroin-based proteins or fragments thereof, wherein die textile or leather product isused for aircraft upholsteiy.

Inan embodiment, the inventionprovides a textile or leather product coated with silk fibroin-based proteins cm- fragments thereof, wherein the textile or leather product is used for upholstery in transportation vehicles for public, commercial, military, or other use, including buses and trains.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile or leather product is used for upholstery of a product that requires a high degree of resistance to wear as compared to normal upholstery.

Inan embodiment, theinventionprovides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as trim on automobile upholstery.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as a steering wheel.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a tortile or leather product fabricated as a headrest.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as an armrest.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as an automobile floor mat.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein thetextileis a textile orleather product fabricated as automobile or vehicle carpet.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as automotive trim.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based protons or fragments thereof, wherein the textile is a 725 textile or leather product fabricated as a children’s car seat In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as a seat belt or safety harness.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein thetextile is a textile or leather product fabricated as a dashboard.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as a seat.

In an embodiment, the invention provides a textile or leather product coated with silk fibroinbased protons or fragments thereof, wherein the textile is a textile or leather product fabricated as a seat panel.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based protans or fragments thereof, wherein the textile is a textile or leather product fabricated as an interior panel.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as an airbag cover.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as an airbag.

In an embodiment, the invention provides a textile or leather product coated with silk fibroin-based proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as a sunvisor.

In an embodiment, the invention provides a textile or leather product coated with silk fibroinbased proteins or fragments thereof, wherein the textile is a textile or leather product fabricated as a wiring harness.

In an embodiment, the invention provides a product coated with silk fibroin-based proteins or fragments thereof, wherein the product is a cushion.

In an embodiment, the invention provides a product coated with silk fibroinbased protons or fragments thereof wherein the product is automotive, aircraft, or other vehicular insulation.

The coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or protein fragments thereof have an average weight average molecular weight range selected from the group consisting of about 5 toabout 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 735 Date re?ue/Date received 2023-07-20 80 kDa, about 60 to about 100kDa, andabout 80kDato about 144kDa, wherein the silk basedproteins ch* fragments thereofhave a polydispersity ofbetween about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity whenin asolutionfor at least 10 days.

Inanembodiment, theinventionprovides an articlecomprising a textileorleather coated with silk fibroin-based proteins or fragments thereof.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of tents, sleeping bags, ponchos, and soft-walled coolers.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of athletic equipment.

In mi embodiment, the textile or leather is atextile or leather usedinthe manufacture of outdoor gear.

Inan embodiment, the textile or leather is a textile or leather usedin the manufacture ofhiking gear, such as harnesses and backpacks.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of climbing gear.

In an embodiment, the textile or leather is canvass.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of ahat.

In an embodiment, the textile or leather is a textile or leather used in themanufacture of anumbrella.

In an embodiment, the textile or leather is atextile or leather used in the manufacture of a tent In an embodiment, the textile or leather is a textile or leather used in the manufacture of a baby sleeper, a baby blanket, or a baby pajama.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of a glove, such as a driving glove or an athletic glove.

In an embodiment, the textile or leather is a textile or leather usedin the manufacture of athletic pants, such as sweat pants, jogging pants, yoga pants, or pants for use in competitive sports.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of athletic shirts, such as sweat shirts, jogging shirts, yoga shirts, or shirts for use in competitive sports.

Inan embodiment, the textile or leather is a textile or leather usedin the manufacture of beach equipment, such as beach umbrellas, beach chain, beach blankets, and beach towels.

In an embodiment, the textile or leatheris atextile or leather usedin themanufacture of jackets or overcoats.

In an embodiment, the textile or leather is a textile or leather used in the manufacture of medical garments, such as surgical drapes, surgical gowns, surgical sleeves, laboratory sleeves, laboratory coals, wound 745 Date regue/Date received 2023-07-20 dressings, sterilization wraps, surgical face masks, retention bandages, support devices, compression bandages, shoe covers, surgical blankets, and the like.

The coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144kDa, wherein the silk based proteins or protein fragments thereofhave an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80kDa to about 144 kDa, whereinthe silkbasedproteins or fragments thereofhave a polydispersity of between about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly changein color or turbidity when ina solution for at least 10 days.

In an embodiment, the invention provides a shoe coated with silk fibroin-based protans ar fragments thereof.

In an embodiment, the invention provides a shoe coated with silk fibroin-based proteins or fragments thereof, wherein the shoe exhibits an improved property relative to an uncoated shoe.

In an embodiment, the invention provides a shoe coated with silk fibroin-based proteins or fragments thereof, wherein the shoe exhibits an improved property relative to an uncoated shoe, and wherein the improved property is stain resistance.

In an embodiment, the invention provides a shoe coated with silk fibroin-based proteins or fragments thereof, wherein the shoe exhibits an improvedproperty relative to an uncoated shoe, and wherein the shoe is made of natural leather or synthetic leather.

The coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk basedproteins or protein fragments thereofhave an average weight average molecular weight range selectedfrom the group consistingof about 5 to about 10 kDa, about 6 kDa to about 16kDa, about 17 kDa to about 38 kDa, about 39 kDato about 80kDa, about 60 to about 1OOkDa, andabout 80kDato about 144kDa, whereinthe silk basedproteins or fragments thereofhave apolydispersity ofbetween about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly change in color ar turbidity whcrnin a solution for at least 10 days. 755 Bate re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight averagemolecular weight range of about 5 kDa toabout 144 kDa.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein the articleis atextileor leather.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or proteinfragments havingabout 0.01%(w/w)to about 10% (w/w) sericin.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragmentsthereof, andcombinationsthereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein tbe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 765 Date re?ue/Date received 2023-07-20 wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based protons or fragments thereof In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silkbasedproteins or fragments comprise silk and a copolymer.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or protan fragments thereof have an average weight average molecular weight range selected from the group consistingof about 5 to about 10 kDa, about 6 kDa to about 16kDa, about 17 kDa to about 38 kDa, about 39 kDato about 80 kDa, about 60 to about 100kDa, and about 80 kDato about 144kDa, whereinthe silk basedproteins or fragments thereofhave apolydispersity ofbetween about 1.5 and about 3.0, and wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gclatc and donot visibly changein color or turbidity whenin a solutionfor at least 10 days.

In an embodiment, die invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprise® silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yarn, or combinations thereof. 775 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber ar yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece,lamawool, cotton, cashmere, sheep fleece, sheep wool, andcombinationsthereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein 1he fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe article is a fabric, wherein the fabric exhibits animproved property, wherein the improved property is an accumulative one-way moisture transport index selected from tite group consisting of greater than 40 %, greater than 60 %, greater than 80 %, greater than 100 %, greater titan 120 %, greater than 140 %, greater titan 160 %, and greater than 180%.

In an embodiment, the foregoing improved property is determined alter a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles,25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprise® silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, wherein the fabric exhibits animproved property, wherein the improved property is an accumulative one way transport capability increase relative to uncoated fabric selected from the group consisting of 1.2 fold, 1.5 fold, 2.0 fold, 3.0 785 Date re?ue/Date received 2023-07-20 fold, 4.0 fold. 5.0 fold, and 10 fold.

In an embodiment, the foregoing improvedpropertv is determined after a period of machine washing cycles selected from die group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average moleculai weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the fabric exhibits animproved property, wherein the improved properly is an overall moisture management capability selected from the group consisting of greater than 0.05, greater than 0.10, greater than 0.15, greater than 0.20, greater than 0.25, greater than 0.30, greater than0.35, greater than 0.40, greater than 0.50, greater than 0.60, greater than 0.70, and greater than 0.80.

In an embodiment, the foregoing improved property is determined ate a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, and wherein the fabric exhibits substantially noincrease in microbial growth ate a number of machine washing cycles selected from die group consistingof 5 cycles, 10 cycles, 25 cycles, and50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die article is a fabric, wherein the fabric exhibits substantially no increase in microbial growth after a number of machine washing cycles selected from die group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles, and wherein the microbial growth is microbial growth of a microbe selected from the group consisting of Staphylococcus aureus,Klebisiettapneumoniae, and combinations thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 795 Date re?ue/Date received 2023-07-20 wherein the article is a fabric, wherein the fabric exhibits substantially no increase in microbial growth after a number of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles, wherein the microbial growth is microbial growth of a microbe selected from the group consisting of Staphylococcus aureus, Klebisiella pneumoniae, and combinations thereof, wherein the microMal growth is reduced by a percentage selected from the group consisting of 50 %, 100 %, 500 %, 1000 %, 2000 %, and 3000 % compared toan uncoated fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the coating is applied to the fabric at the fiber level priorto forming the fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the coating is applied tothe fabric atthe fabric level or garment level (e.g., after manufacture of a garment from fabrics, leathers, and/or other materials).

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level or garment level, and wherein the fabric is bath coated.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, w herein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the artide is a fabric, wherein the coating is applied to 1he fabric at the fabric level or garmentlevel, and wherein the fabric is spray coated.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 805 Date re?ue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level or garmentlevel,and whereinthe fabric iscoatedwitha stencil.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level or garment level, and wherein the coatingis applied to at least one side ofthe fabric using a method selected from the group consisting of a bath coating process, a spray coating process, a stencil (i.e., screen) process, a silk-foam based process, a roller-based process, a magnetic idler process, a knife process, a transfer process, a foam process, a lacqueringprocess, and a printing process.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied tothe fabric at the fabric level, and wherein the coating is applied to both sides of the fabricusing amethod selected from the group consisting of abath coating process, a spray coating process, a stencil (i.e., screen) process, a silk-foam based process, a roller¬ based process, a magnetic roller process, a knife process, a transfer process, a foam process, alacqueringprocess, and aprintingprocess.

In any of the foregoing embodiment, the coating may be applied at the fabric garment level by any of the methods disclosedhereinto recondition fabrics or garments.

For example, such reconditioning using a coating comprising silk based proteins or fragmentsthereofmay be performed as part ofwashingor cleaninga fabric or garment.

In an embodiment, tiie invention provides an article comprising a fiber or yam having a coating, wherein the coating comprise® silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein thecoating has a thickness of about onenanolayer.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 815 Bate re?ne/Bate received zOzS-Oz-zO having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein the coating has a thickness selected from the group consisting of about 5 nm, about 10 nm, about 15 nm, about 20nm, about 25 nm, about 50 nm, about 100nm, about 200nm, about 500nm, about1gm, about 5 gm, about 10 urn, andabout 20 gm.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis afabric, and whereinthe coatingis adsorbed on the fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the coating is attached to the fabric through chemical, enzymatic, thermal, or irradiative cross-linking.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein the hand of the coated fabric is improved relative to an uncoated fabric.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein the hand of the coated fabric is improved relative to an uncoated fabric, wherein the hand of the coated fabric that is improved is selected from the group consistingof softness, crispness, dryness, silkiness, andcombinations thereof.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 825 Date re?ue/Date received 2023-07-20 wherein the article is a fabric, wherein the coating is applied to the fabric at the fabric level, and wherein the pilling of thefabricis improved relative to an uncoated fabric.

In an embodiment, the silk coating is applied using a bath process, a screen (or stencil) process, a spray process, a silk-foam based process, and a roller based process.

In an embodiment, a fiber or a yam comprises a synthetic fiber or yam, including polyester.Mylar, cotton, nylon, polyester-polyurethane copolymer, rayon, acetate, aramid (aromatic polyamide), acrylic, ingeo (polylactide), lurex (polyamide-polyester), olefin (polyethylene-polypropylene), and combinations thereof.

In an embodiment, a fiber or a yam comprises a natural fiber or yam (e.g., from animal or plant sources), including alpaca fiber, alpaca fleece, alpaca wool, lama fiber, lama fleece, lama wool, cotton, cashmere and sheep fiber, sheep fleece, sheep wool, byssus, chiengora, quiviut, yak, rabbit, lambswool, mohair wool, camel hair, angora wool, silkworm silk, abaca fiber, coir fiber, flax fiber, jute fiber, kapok fiber, kenaf fiber, raffia fiber, bamboo fiber, hemp, modal fiber, pina, ramie, sisal, and soy protein fiber.

In an embodiment, a fiber or a yam comprises a mineral fiber, also known as mineral wool, mineral cotton, or man-made mineral fiber, including fiberglass, glass, glasswool, stone wool, rock wool, slagwool, glass filaments, asbestos fibers, and ceramic fibers.

In an embodiment, a water-soluble silk coating may be used as an adhesive or binder for binding particles tofabrics or for binding fabrics.

In an embodiment, an article comprises a fabric bound to another fabric using a silk coating.

In an embodiment, an article comprises a fabric with particles bound tothe fabric tiring a silk adhesive.

In an embodiment, the coating is applied to an article including a fabric at the yam level.

In an embodiment, the coating is applied at the fabric level.

In an embodiment, the coating has a thickness selected from the group consisting of about 5 nm, about 10 nm, abort 15 nm, about 20 nm, about 25 nm, about 50 nm, about 100 nm, abort 200 nm, about 500 nm, about 1 pm, about 5 pm, about 10 pm, and abort 20 pm.

In an embodiment, the coating has a thickness range selected from the group consisting of about 5 nm to about 100 nm, about 100 nm to about 200 nm, about 200 nm to about 835 Date re?ue/Date received 2023-07-20 500 nm, about I gm to about 2 gm, about 2 gm to about 5 gm, about 5 gm to about 10 gm, and about 10 gm to about 20 gm.

In an embodiment, a fiber or a yam is treated with a polymer, such as polyglycolide (PGA), polyethylene glycols, copolymers of glycolide, glycolide/L-lactide copolymers (PGAZPLLA), glycolide/trimethylene carbonate copolymers (PGA/TMC), polylactides (PLA), stereocopolyniers of PLA, poly-L-lactide (PLLA), poly-DL-lactide (PDLLA), L-lactide/DL-lactide copolymers, co-polymers of PLA, lactide/tetramethylglycolide copolymers, lactide/trimethylene carbonate copolymers, laclide/b-valerolactone copolymers, lactide/E-caprolactone copolymers, polydepsipeptides, PLA/polyethylene oxide copolymers, unsymmetrically 3,6-substituted poly-1,4-dioxane-2,5-diones, poly-0-hydroxybutyrate (PHBA), PHBA/0-hydroxyvalerate copolymers (PHBA/HVA), poly-0-hydroxypropionate (PHPA), poly-p-dioxanone (PDS), poly-5-valerolactone, poly-e-caprolactone, methylmethacrylate-N-vinyl pyrrolidine copolymers, polyesteramides, polyesters of oxalic acid, polydihydropyrans, pdyalkyl-2- cyanoaciylates, polyurethanes (PU), polyvinylalcohols (PVA), polypeptides, poly-0- malic acid (PMLA), poly-0-alkanoic acids, polyvinylalcohol (PVA), polyethyleneoxide (PEO), chitine polymers, polyethylene, polypropylene, polyasetal, polyamides, polyesters, polysulphone, polyether ether ketone, polyethylene terephthalate, polycarbonate, polyaryl ether ketone, and polyether ketone ketone.

In an embodiment, the silk coating surface can be modified siIk crystalsthat range in size from nm to gm.

The criterion for “visibility” is satisfied by any one of the following: a change in the surface character of the textile; the silk coating fills the interstices where the yams intonsect; or the silk coating blurs or obscures the weave.

In an embodiment, a silk based protein or fragment solution may be utilized to coat at least a portion of a fabric which can be used tocreate a textile.

In an embodiment, a silk based protein or fragment solution may be weaved into yam that can be used as a fabric in a textile.

In an embodiment, a silk based protein or fragment solution may be used to coat a fiber.

In an embodiment, the invention provides an article comprising a silk based protein or fragment solution coating at least a portion of a fabric or a textile. 845 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a silk based protein or fragment solution coating a yam.

In an embodiment, the invention provides an article comprisinga silk based protein or fragment solution coating a fiber.

There is disclosed a textile that is at least partially surface treated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure so as to result in a silk coating on the textile.

In an embodiment, the silk coating of the present disclosure is available in a spray can and can be sprayed on any textile by a consumer.

In an embodiment, a textile comprising a silk coating of the present disclosure is sold to a consumer.

In an embodiment, a textile of the present disclosure is used in constructing action sportswear/apparel In an embodiment, a silk coating of the present disclosure is positioned on the underlining of apparel.

In an embodiment, a silk coating of the present disclosure is positioned on die shell, the lining, or the interlining of apparel.

In an embodiment, apparel is partially made from a silk coated textile of the present disclosure and partially made from an uncoated textile, In an embodiment, apparel partially made from a silk coated textile and partially made from an uncoated textile combines an uncoated inert synthetic material with a silk coated inert synthetic material.

Examples of inert synthetic material include, but are not limited to, polyester, polyamide, polyaramid, polytetrafhiorethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethylenglycol, ultrahigh molecular weight polyethylene, highperformance polyethylene, and mixtures thereof.

In an embodiment, apparel partially made from a silk coated textile and partially made from an uncoated textile combines an elastomeric material at least partially covered with a silk coating of the present disclosure.

In an embodiment, the percentage of silk to elastomeric material can be varied to achieve desired shrink or wrinkle resistant properties.

In an embodiment, a silk coating of the present disclosure is visible.

In an embodiment, a silk coating of the present disclosure positioned on apparel helps control skin temperature.

In an embodiment, a silk coating of the present disclosure positioned on apparel helps control fluid transfer away from the skin.

In an embodiment, a silk coating of the present disclosure positioned cm apparel has a soft feel against the skin decreasing abrasions from fabric on skin.

In an embodiment, a silk coating of the present disclosure positioned on a textile has properties that confer at least one of wrinkle 855 Date re?ue/Date received 2023-07-20 resistance, shrinkage resistance, or machine washability to the textile In an embodiment, a silk coated textile of the present disclosure is 100% machine washable and dry cleanable.

In an embodiment, a silk coated textile of the present disclosure is 100% waterproof.

In an embodiment, a silk coated textile of the present disclosure is wrinkle resistant, hi an embodiment, a silk coated textile of the present disclosure is shrink resistant.

In an embodiment, a silk coated textile of the present disclosure has the qualities of being waterproof, breathable:, and elastic and possess a number of other qualities which are highly desirable in action sportswear.

In an embodiment, a silk coated textile of the present disclosure manufactured from a silk fabric- of the present disclosurefurtherincludes LYCRA® brand spandex fibers.

In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a breathable fabric.

In an embodiment, a textile at leastpartially coated with an aqueous solution ofpure silk fibroin-based protein fragments ofthe present disclosure is a water-resistant fabric.In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a shrink-resistant fabric.In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments ofthe present disclosureis amachine-washablefabric.In an embodiment, a textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is a wrinkle resistant fabric.

In an embodiment, textile at least partially coated with an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure provides moisture and vitamins to the skin.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present disclosureis used to coat a textile or leather.

In an embodiment, the concentration of silk in the solution ranges from about 0.1% to about 20.0%.

In an embodiment, the concentration of silk In the solution ranges from about 0.1% to about 15.0%.

In an embodiment, the concentration of silk in the solution ranges from about 0.5%to about 10.0%.

In an embodiment, the concentration of silk in the solution ranges from about 1.0% to about 5.0%.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure is applied directly to a fabric. 865 Date re?ue/Date received 2023-07-20 Alternatively. silk mioosphae and any additives may be used for coating a fabric.

In an embodiment, additives can be added to an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure before coating (e.g., alcohols) to further enhance material properties.

In an embodiment, a silk coating of 1he present disclosure can have a pattern to optimize properties of the silk on the fabric.

In an embodiment, a coating isapplied to a fabric under tension and/or lax to vary penetration into thefabric.

In an embodiment, a silk coating of the present disclosure can be applied at the yam level, followed by creation ofa fabric once the yam is coated.

In an embodiment, an aqueous solution of pure silk fibroin-based protein fragments of the present disclosure can be spun into fibers to make a silk fabric and/or silk fabric blend with other materials known in the apparel industry.

Uges of...Textil.es...and Leafers Coated,..with Silk FibrpifcBasgd Apparel and Garment Applications In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article exhibits an improved color retention property.

Without bong bound by any specific theory, it is postulated that the coating prevents the article from color degradation by separating thefiber or yam from air or from detergents during washing.

Methods of testing the color retention property of an article are well within the knowledge of one skilled in the art. A specific method of testing of the color retention property of a fabric is described in U.S.

Patent No. 5,142,292.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits an improved color retention property.

In an embodiment, die invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 875 Date re$ue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from 1he group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from tire group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein tire silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that ate selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural 885 Date re?ue/Date received 2023-07-20 silk based proteins or fragments are silkworm silk based proteins or fragments thereof and die silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article exhibits an improved color retention property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits an improved color retention property.

In an embodiment, the foregoing color retention property of the fabric is 895 Date re?ue/Date received 2023-07-20 determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits an improved color retention property.

In an embodiment, the foregoing improved color retention property of the textile is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fibrar or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is resistant to microbial (including bacterial and fungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is resistant to microbial (including bacterial and fungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article is resistant to microbial (including bacterial and fungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof wherein the article is resistant to microbial including bacterial and fungal) growth. 905 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof wherein the article is resistant tomicrobial (includingbacterial and fungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein the article is resistant to microbial (including bacterial and fungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silkbased proteins or fragments comprise silk and a copolymer, wherein the artideisresistant to microbial(indudingbacterial andfungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 915 Date re?ue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein thearticleis resistant to microbial(includingbacterial andfungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article is resistant to microbial(includingbacterial and fungal) growth.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is resistant to microbial (including bacterial and fungal) growth.

In an embodiment, the foregoing resistant to microbial (includingbacterial and fungal) growth property of the fabric is determined after aperiod of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits resistant to microbial (including bacterial and fungal) growth property.

In an embodiment, the foregoing resistant to microbial (including bacterial and fungal) growth property of the textile is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 925 Date re?ue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein thearticleis resistant to thebuildup of static electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wirerein the article is resistant to the buildup of static electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating; wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article isresistant to thebuildup ofstatic electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article is resistant to thebuildup of static electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof recombinant silk based proteins ar fragments thereof, and combinations thereof, wherein 1he silk based proteins or fragments thereof are natural silk basedprotans or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof, wherein the article is resistant tothe buildup of static electrical charge. 935 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein die article is resistant to the buildup of static electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the articleisresistant to thebuildup ofstatic electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof’ wherein the fiber or yam is natural fiber or yarn selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein thearticleisresistantto thebuildup of static electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die fiber or yam is selected from the group consisting of natural fiber or yam, 945 Date re?ue/Date received 2023-07-20 synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article is resistant to the buildup of static electrical charge.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is resistant to the buildup of static electrical charge.

In an embodiment, the foregoing resistant to the buildup of static electrical charge property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits resistant to the buildup of static electrical charge property.

In an embodiment, the foregoing resistant to the buildup of static electrical charge property of the textile is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yarn having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article is mildew resistant. 955 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof. wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedprotoms or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof, wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk basedproteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein ihe natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein the articleis mildew resistant. 965 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article is mildew resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is mildew resistant.

In an embodiment, the foregoing mildew resistant property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits mildew resistant property.

In an embodiment, tire foregoing mildew resistant property of the 975 Date re?ue/Date received 2023-07-20 textile is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein thecoaling istransparent.

In an embodiment, the invention provides an article comprising a fibrar or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range Of about 5 kDa to about 144 kDa, wherein the coating istransparent.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the coating istransparent.

In an embodiment, the invention provides an article comprising a fibs' or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based protons or fragments thereof, and combinations thereof, wherein the coating is transparent In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that are selected 985 Date re?ue/Date received 2023-07-20 from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the coating is transparent In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein tire silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and tiie silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins orfragments thereof, wherein the coating is transparent.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the coating is transparent.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber ar yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, w herein the coating is transparent.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 995 Date regue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, andcombinations thereof, whereinthe coatingis transparent.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating is transparent.

In an embodiment, the foregoing transparent property of the coating is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather comprises a silk coating of the present disclosure, wherein the silk coating is transparent.

In an embodiment, the foregoing transparent property of the coating is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleisresistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is resistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins 1005 Date re?ue/Date received 2023-07-20 or protein fragments having about 0.01%(w/w) to about 10% (w/w) sericin, wherein the articleisresistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein die article is resistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from die group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins ar fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof, wherein the article is resistant tofreeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based protans or fragments thereof, and combinations thereof, wherein die silk basedproteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based protans or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based protans or fragments thereof is Bombyx mori silk based 1015 Date re?ue/Date received 2023-07-20 proteins or fragments thereof, wherein the article is resistant to freeze-thaw cycle damage In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article is resistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yarn, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article is resistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article is resistant to freeze-thaw cycle damage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is resistant to freeze-thaw cycle damage.

In an embodiment, the foregoing resistant to freeze-thaw cycle damage property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles. 1025 Date re?ue/Date received 2023-07-20 In an embodiment, a textile or leather of the present disclosure exhibits resistant to freeze-thaw cycle damage.

In an embodiment, the foregoing resistant to freeze-thaw cycle damage property of the textile is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the coating provides protection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating provides protection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the coating provides protection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof and combinations thereof, wherein the coating provides protection from abrasion In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof recombinant silk based 1035 Date re?ue/Date received 2023-07-20 proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that ate selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the coaling providesprotection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins ar fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkw orm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein thecoatingprovidesprotection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam hatring a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the coating providesprotection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber ar yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the coating providesprotection from abrasion. 1045 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the coating provides protection from abrasion.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the coating provides protection from abrasion.

In an embodiment, the foregoing abrasion resistant property of die fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits abrasion resistant.

In an embodiment, the foregoing abrasion resistant property of the textile is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article exhibits the property' of blocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits the property of blocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 1055 Bate re?ne/Bate received zOzS-Oz-zO having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article exhibitsIheproperty ofblocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from 1he group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article exhibits the property ofblockingultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yarn having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant talk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that ate selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the article exhibits theproperty ofblocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural 1065 Date re?ue/Date received 2023-07-20 silk based proteins or fragments are silkworm silk based proteins or fragments thereof and die silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein the article exhibits the property of blocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article exhibits the property of blocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article exhibits the property of blocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article exhibits the property of blocking ultraviolet (UV) radiation.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits the property of blocking ultraviolet (UV) radiation.

In an embodiment, the foregoing UV blocking property of the 1075 Date re?ue/Date received 2023-07-20 fabric is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits UV blocking property.

In an embodiment, the foregoing UV blocking property of die textile is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the garment regulates the body temperature of a wearer.

In an embodiment, the invention provides a garment comprising a fiber or yarn having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the garment regulates the body temperature of a wearer.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the garment regulatesthe body temperature of a wearer.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereo£ and combinations thereof, wherein the garment regulates the body temperature of a wearer. 1085 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides a garment comprising a fibs’ or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the garment regulates the body temperature of a wearer.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein the garment regulates the body temperature of a wearer.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the garment regulatesthe body temperature of a wearer.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 1095 Date re?ue/Date received 2023-07-20 having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein thegarment regulates thebody temperature ofa wearer, In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the garment regulates the body temperatureof awearer.

In an embodiment, the invention provides a garment comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the garment regulates the body temperature of a wearer.

In an embodiment, the foregoingtemperature regulation property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cyclo, 10 cycles,25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits a temperatureregulationproperly.

Inan embodiment, the foregoingtemperatureregulation property of the textile is determined after a period of machine washing cycles selected fromthe group consisting of5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, and wherein the articleis tear resistant. 1105 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the article istear resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, and wherein the articleis tear resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, and wherein the article is tear resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, and wherein the article is tear resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 1115 Date regue/Date received 2023-07-20 wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof and whereinthe articleistearresistart.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silkbasedproteins or fragments comprise silk and a copolymer, and wherein the articleis tear resistant, In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber car yam, or combinations thereof, wherein the fiber or yam is natural fiber or yarn selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, and whereinthe articleis tearresistant, In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein die fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber w yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, and wherein the article is tear resistant, 1125 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, and whereindie articleis tear resistant.

Inan embodiment, the foregoing tear resistant property of the fabric is determined after a period ofmachine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits a tear resistant property.

In an embodiment, the foregoing tear resistant property of the textile is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein theelasticity ofthe articleisimproved.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the elasticity ofthe articleisreduced.

In an embodiment, the invention provides an article comprising a flbar or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the elasticityofthe articleis improved.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins 1135 Date re?ue/Date received 2023-07-20 or protein fragments having about 0.01%(w/w) to about 10% (w/w) sericin, wherein the elasticity ofthe articleis reduced.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article exhibits arebound dampeningproperty.

Without being boundby any specific theory,it is postulated that the coating prevents the article from returning to the original shape or orientation, and resultsinthe rebounddampeningproperty.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein thearticleis afabric, wherein the article exhibits arebound dampeningproperty.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-basedproteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article exhibits arebound dampening property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof and combinations thereof, wherein the article exhibits a rebounddampening property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based 1145 Date re?ue/Date received 2023-07-20 proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that ate selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein die article exhibits arebound dampeningproperty.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins ar fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkw orm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein thearticle exhibits a rebounddampeningproperty.

In an embodiment, the invention provides an article comprising a fiber or yam hatring a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article exhibits arebound dampeningproperty.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber ar yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein thearticle exhibits arebound dampeningproperty. 1155 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article exhibitsa rebound dampening property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits a rebound dampening property.

In an embodiment, the foregoing rebound dampening property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits a rebound dampening property, hi an embodiment, the foregoing rebound dampening properly of the textileis determined aftera period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, w'herein the article is a fabric, wherein the article exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yarn having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 1165 Date re?ue/Date received 2023-07-20 wherein the silk based protans or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein die article exhibits an anti-itch properly.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the article exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from die group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein die silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein die natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, 1175 Date re?ue/Date received 2023-07-20 and the alkwonn silk based proteins or fragments thereof is Bombyx mori silk based protans orfragments thereof, wherein thearticle exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama w ool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article exhibits an anti-itch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article exhibits an antiitch property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits an anti-itch property.

In an embodiment, the foregoing anti-itch property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles. 1185 Date re?ue/Date received 2023-07-20 In an embodiment, a textile or leather of the present disclosure exhibits an antiitch property.

In an embodiment, the foregoing anti-itch property of the textile is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article exhibits an improved insulation/wannth property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article exhibits an improved insulation/wannth property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article exhibits an improved insulation/wannth property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article exhibits an improved insulation/wannth property.

In an embodiment, tire invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group 1195 Date re?ue/Date received 2023-07-20 consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof silkworm silk based proteins ar fragments thereof, and combinations thereof, wherein the article exhibits animprovedinsulation/warmth properly.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based protons or fragments thereof, and combinations thereof, wherein the silk basedproteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein the article exhibits animproved insulation/warmth property.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, wherein the article exhibits animproved insulation/warmth property.

In an embodiment, the foregoing improved insulation/warmth property of the fabric is determined after a period of machine washing cycles selected from 1he group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

Inan embodiment, a textile or leather ofthe present disclosure exhibitsimproved an insulation/warmth property.

In an embodiment, the foregoing improved insulation/warmth property ofthe textileis determined after a period ofmachine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles. 1205 Date regue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die article is a fabric, wherein the article is wrinkle resistant In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm 1215 Date re?ue/Date received 2023-07-20 silk based proteins or fragments thereof, and combinations thereof, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk based proteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins orfragments thereof, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, syntheticfiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 1225 Date re?ue/Date received 2023-07-20 wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article is wrinkle resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, wherein the article is wrinkleresistant.

In an embodiment, the foregoing wrinkle resistant property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits wrinkle resistant property.

In an embodiment, the foregoing wrinkle resistant property of the textile is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis stain resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, wherein the articleis stainresistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the articleis stain resistant. 1235 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein ihe silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article is stain resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from ihe group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk based proteins or fragments thereof are natural silk basedprotoms or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof, wherein the article is stainresistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the silk basedproteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein ihe natural silk based proteins or fragments are silkworm silk based proteins or fragments thereof, and the silkworm silk based proteins or fragments thereof is Bombyx mori silk based proteins or fragments thereof, wherein thearticleis stainresistant. 1245 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk basedproteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the articleis stain resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the articleis stain resistant.

In an embodiment the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, andcombinations thereof, whereinthe article is stainresistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, wherein the articleis stainresistant.

In anembodiment, the foregoing stain resistant property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits stain resistant property.

In an embodiment, the foregoing stain resistant property of the textile 1255 Date re?ue/Date received 2023-07-20 is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis sticky.

Without being bound to any specific theory,it is postulated thatthe coatingprovides stickiness and maintains stickiness.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis afabric,wherein the articleis sticky.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins or protein fragments having about 0.01% (w/w) to about 10% (w/w) sericin, wherein the articleis sticky.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the article is sticky.

In an embodiment, the foregoing sticky property of the fabric is determined after a period of machine washing cycles selected from the group consistingof 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

In an embodiment, a textile or leather of the present disclosure exhibits sticky property.

In an embodiment, the foregoing sticky property of the textile is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles,25 cycles, and 50 cycles.

Inan embodiment, theinventionprovides an articlecomprising a textileor leather coated with silk fibroin-based proteins or fragments thereof, wherein the article exhibits 1265 Date re?ue/Date received 2023-07-20 improved flame resistance relative to an uncoated textile.

In an embodiment, the inventionprovides an article comprising a textile or leather coatedwith silk fibroin-based proteins or fragments thereof, wherein the article exhibits equal flame resistance relative to an uncoated textile or leather, hi an embodiment, the invention provides an article comprising a textile or leather coated with silk fibroin-based proteins or fragments thereof, wherein the article exhibits equal flame resistance relative to anuncoated textile or leather, wherein an alternative textile or leather coating exhibits reduced flame resistance.

In an embodiment, the invention provides an article comprising a textile or leather coated with silk fibroin-based proteins or fragments thereof, wherein the article exhibits improved resistance to fire relative to an uncoated textile or leather, wherein the improved resistance to fire is determinedby a flammability test, In an embodiment, the flammability test measures afterflame time, afterglow time, char length, and the observation of fabric meltingor dripping.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis flame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range Of about 5 kDa to about 144 kDa, wherein the articleis a fabric, wherein the articleis flame resistant.

Inanembodiment, theinventionprovides an articlecomprising apolyesterhaving a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleisflame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof comprise silk fibroin-based proteins 1275 Date re?ue/Date received 2023-07-20 or protein fragments having about 0.01%(w/w) to about 10% (w/w) sericin, wherein the article is flame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from the group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins or fragments thereof, and combinations thereof, wherein the article is flame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from 1he group consisting of natural silk based proteins or fragments thereof, recombinant silk based proteins ar fragments thereof, and combinations thereof, wherein die silk based proteins or fragments thereof are natural silk basedproteins or fragments thereof that are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk basedproteins or fragments thereof, and combinations thereof, wherein the article is flameresistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof are selected from die group consisting of natural silk based proteins or fragments thereof, recombinant silk based protans or fragments thereof, and combinations thereof, wherein the silk basedproteins or fragments thereof are natural silk basedproteins or fragments thereofthat are selected from the group consisting of spider silk based proteins or fragments thereof, silkworm silk based proteins or fragments thereof, and combinations thereof, wherein the natural silk based protans or fragments are silkworm silk based proteins or fragments thereof, 1285 Date re?ue/Date received 2023-07-20 and the alkwonn silk based proteins or fragments thereof is Bombyx mori silk based proteins orfragments thereof, wherein thearticle is flame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the silk based proteins or fragments comprise silk and a copolymer, wherein the article isflame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is natural fiber or yam selected from the group consisting of cotton, alpaca fleece, alpaca wool, lama fleece, lama w ool, cotton, cashmere, sheep fleece, sheep wool, and combinations thereof, wherein the article is flame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein Ilie fiber or yam is selected from the group consisting of natural fiber or yam, synthetic fiber or yam, or combinations thereof, wherein the fiber or yam is synthetic fiber or yarn selected from the group consisting of polyester, nylon, polyester¬ polyurethane copolymer, and combinations thereof, wherein the article is flame resistant.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, wherein the fabric is flame resistant In an embodiment, the foregoing flame resistant property of the fabric is determined after a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles. 1295 Date re?ue/Date received 2023-07-20 Inan embodiment, atextile or leather of the present disclosure is flame resistant.

In anembodiment, the foregoing flame resistantproperty ofthe textileis determinedafter a period of machine washing cycles selected from the group consisting of 5 cycles, 10 cycles, 25 cycles, and 50 cycles.

Inan embodiment, the inventionprovides a leather coated with coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the leather exhibits an property selected from the group consisting of an improved color retention property, improvedmildewresistance,improved resistance to freeze-thaw cycle damage,improved resistance to abrasion, improved blocking of ultraviolet (UV) radiation, improved regulation of the body temperature of a wearer, improved tear resistance, improved elasticity, improved rebound dampening, improved anti-itch properties, improved insulation, improved wrinkle resistance, improved stain resistance, and improved stickiness.

In an embodiment, the invention provides a leather coated with coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the coating is transparent In any of the foregoing embodiments, at least one property of the article is improved, wherein the property that is improvedis selected from1he group consisting of color retention, resistance to microbial growth, resistance to bacterial growth, resistance to fungal growth, resistance to the buildup of static electrical charge, resistance to the growth ofmildew, transparency of the coating, resistance to freeze-thaw cycle damage, resistance from abrasion, blocking of ultraviolet (UV) radiation, regulation of the body temperature of a wearer, resistance to tearing, elasticity of the article, rebound dampening, tendency to cause itching in the wearer, thermal insulation of the wearer, wrinkle resistance, stain resistance, stickiness to skin, and flame resistance, and wherein the property is improved by an amount relative to an uncoated article selected from the group consisting of at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at 1305 Date re?ue/Date received 2023-07-20 least 95°i, at least 100%, at least 125%, at least 150%, at least 200%, at least 300%, at least 400%, and at least 500%.

In any of the foregoing embodiments, the silk based proteins or protein fragments thereof have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa. about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gdate and do not visibly change in color or turbidity when in a solution for at least 10 days.

Additional Agents, for., Use..with,„Te^les .Coated,.,with Silk Jibroin-Based Protein Fragments In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is pretreated with a wetting agent.

In an embodiment, the wetting agent improves one or more coating properties.

Suitable wetting agents are known to those of skill in the art.

Exemplary, non-limiting examples of wetting agents from a representative supplier, Lamberti SPA, are given in the following table.

Imbitex NDT Non silicone low foaming with high wetting in both hot or cold conditions, with good detergency and good stability to alkalis.

Imbitex TBL Wetting and de-aerating agent.

Imbitex MRC Wetting and penetrating agent for mercerizing of cotton.

Tensdam Na liq Low foam, special wetting and dispersing agent for non¬ woven wet treatments Imbitex \RW3 Wetting agent for water-and oil repellent finishing.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and w'herein the fabric is pretreated with a detergent.

In an 131embodiment the detergent improves one or more coating properties.

Suitable detergents are known to those of skill in the art.

Exemplary, non-limiting examples of detergents froma representative supplier,Lambert! SPA, are giveninthe followingtable.

BiordCPNN Wettingand detergent agent withalkaline stability inNaOH up to 10° C.

Recommended for continuous scouring, bleaching, and Jigger applications.

BiordJK new Wettinganddetergent agent with extremelylow foam properties, recommended for high bath turbulencemachine (e.#., jet, overflow,etc}.

Biord OW 60 General-purpose wettingand detergent agent suitable for desizing, scouring, andbleaching processes.

Biord OWK Detergent /wettingagent,low foaming, high concentration, recommended for over-flow.

Useful for removal of silicone oil onLycrfblends.

Cesapon Silk liq.

Specific scouring, de-gumming agent for silk.

CesaponExtra Highdetergent power product containing sdvent. 5 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is pretreated with a sequestering or dispersingagent Suitable sequestering or dispersing agents are known to thoseof skillin 10 the art.

Exemplary, non-limiting examples of sequestering or dispersing agents from a representative supplier,Lamberti SPA, are givenin the following table.

Lamqgal DSP Dispersingand anti-redepositing agent usefill for preparation dyeingand after soapingof dyedandprintedmaterials with reactive andvat dyes.

This product is alsouseful as an antiolygomer agent inreduction clearingof polyester, dyedor printedwith disperse dyes.

Chelam TLW/T Multi-purpose sequestring anddispersing agent for a wide variety of textileprocesses.

No shade variation on dyestuff containingmetals.

Lamegal TL5 Multi-purpose sequestring anddispersing agent for a wide variety oftextile processes 132 Bate re?ae/Bate received5 Date re?ue/Date received 2023-07-20 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is pretreated with an enzyme.

Suitable enzymes are known to those of skill in the art.

Exemplary, non-limiting examples of enzymes from a representative supplier, Lamberti SPA, are given in the following table.

LazimHT Thermo-stable amylase for rapidhightemperature desizing.

LazimPE Specific enzyme for bioscouring; provides optimal wettability, it improves dyeing and color fastness without causing depolimerization and fabric strength loss.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is pretreated with a bleaching agent.

Suitable bleaching agents are known to those of skill in the art.

Exemplary, non-limiting examples of bleaching agents from a representative supplier, Lamberti SPA, are given in the following table.

Stabilox OTN cone.

Highly concentrated stabilizerfor alkaline bleaching with hydrogen peroxide.

Suitable for a wide variety of processes.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is pretreated with an antifoaming agent.

Suitable antifoaming agents are known to those of skill in the art.

Exemplars, non-limiting examples of antifoaming agents from a representative supplier, Lamberti SPA, are given in the following table.

Antifoam SE 47 General purpose defoaming agent.

Defomex JET Silicone defoamer effective up to 130° C.

Recommended for HT and JET dyeing systems. 133|Defomex 2033 |Non-siliconedefoamer.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 5 wherein the article is a fabric, and wherein the fabric is pretreated with an anti-creasing agent.

Suitable anti-creasing agents are known to those of skill in the art.

Exemplary, non-limiting examples of anti-creasing agents from a representative supplier, Lamberti SPA, aregiven in the following table.

Lubisol AM Lubricating and anti-creasing agent for rope wet operation on all kind of fibers and machines. 10 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is treated with a dye dispersing agent.

Suitable dye dispersing agents are known to those of skill in the art.

Exemplary, 15 non-limiting examples of dye dispersing agents from a representative supplier, Lamberti SPA, aregiven in the following table.

Lamegal BO Liquid dispersing agent (non-ionic), suitable for direct, reactive, disperse dyeing and PES stripping.

Lamegal DSP Dispersing and anti back-staining agent in preparation, dyeing and soaping of dyed and printed materials.

Antioligomer agent Lamegal 619 Effective lowfoam dispersing leveling agent for dyeing of PES.

Lamegal TL5 Multi-purpose sequestering and dispersing agent for a variety of textile processes.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof 20 having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is treated with a dye leveling agent. 134 Bate re?ne/Bate received 2023-07-26Suitable dye leveling agents are known to those of skill in the art.

Exemplary, non¬ limiting examples of dye leveling agents from a representative supplier, Lamberti SPA, are givento the following table.

Lamegal A 12 Leveling agent for dyeingon wool, polyamide anditsblends withadd or metal complex dyes. 5 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular w eight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is treated with a dye fixing agent.

Suitable dye fixing agents areknown to those of skill to the art.

Exemplary, non-limiting 10 examples of dye fixing agents from a representative supplier,Lamberti SPA, are givenin the following table.

Lamfix 1/ Fixingagent for direct andreactive dyestuffs, containing formaldehyde.

Lamfix LUcone.

Formaldehyde free cationic fixingagent for direct andreactive dyes.It doesnot affect the shade andlight fastness.

Lamfix PA/TR Fixingagentto improve the wet fastness ofadd dyes onpolyamide fabrics, dyedor printed and polyamide yams.

Retarding agentin dyeingof Polyamide/cellulosicblends withdirect dyes.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 15 having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is treated with a dye special resin agent.

Suitable dye special resin agents areknown to thoseof skillin the art.

Exemplary, non-limiting examples of dye special resin agents from a representative supplier, Lamberti SPA, are givenin the followingtable.

DenifastTC Special resin for cationization ofcellulase fibers to obtain special effects ("DENIFAST system" and "DENISOL system"). 135 Bate re?ue/Date received 2023-07-205 Bate regWBate receiver! CobralDD/50 Specialresin for cationization ofcellulose fibers to obtain special effect ("DENIFAST system" and "DENISOL system").

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is treated with a dye anti-reducing agent.

Suitable dye anti-reducing agents are known to those of skill in the art.

Exemplary, non-limiting examples of dye anti-reducing agents from a representative supplier,Lamberti SPA, are giveninthe following table.

LambertiRedoxL2S gra Anti-reducing agentingrain form. 100% active content.

LambertiRedox L2S liq.

Anti-reducing agentinliquid form for automatic dosage.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the articleis a fabric, andwhereinthe fabric istreated with a pigment dye system anti-migrating agent.

Suitable pigment dye system anti-migrating agents are known to those of skill in die art.

Exemplary, non-limiting examples of pigment dye system anti¬ migratingagents fromarepresentative supplier,Lamberti SPA, aregivenin thefollowing table.

Neopat Compound 96An cone.

Compound, developed as migration inhibitor fir continuous dyeing process with pigments (paddry process).

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range <£ about 5 kDa to about 144 kDa, wherein the articleis afabric, and whereinthe fabric istreated with apigment dye system binder.

Suitable pigment dye system binders are known to those of skill in the art. 1365 re?ae?Bate received 2&23-V7-2Q Exemplary, non-limiting examples of pigment dye system binders from a representative supplier, Lamberti SPA, are given in the following table.

Neopat Binder PM/S cone.

Concentrated version of a specific binder used to prepare pad-liquor for dyeing with pigments (pad-dry process).

In an embodiment, die invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein die article is a fabric, and wherein the fabric is treated with a pigment dye system binder and anti-migrating agent combination.

Suitable pigment dye system binder and anti-migrating agent combinations are known tothose of skill in the art.

Exemplary, non¬ limiting examples of pigment dye system binder and anti-migrating agent combinations from a representative supplier, Lamberti SPA, are given in the following table.

Neopat Canpound PK1 Highly concentrated all-in-one product specifically developed as migration inhibitor with specific binder for continuous dyeing process with pigments (pad-dry process).

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is treated with a delave agent.

Suitable delave agents are known to those of skill in the art.

Exemplary, non-limiting examples of delave agents from a representative supplier, Lamberti SPA, are given in the following table.

Neopat compound FTN Highly concentrated compound of surfactants and polymers specifically developed for pigment dyeing and pigment-reactive dyeing process; especially for medium/dark shades for wash off effect. 137In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is traditionally finished with a 5 wrinkle free treatment.

Suitable wrinkle free treatments are known to those of skill in the art.

Exemplary, non-limiting examples of wrinkle free treatments from a representative supplier, Lamberti SPA, are given in the following table.

Cellofix ULF cone.

Anti-crease modified glyoxalic resin for finishing of cottons, cellulosics and blends with synthetics fibers.

PoliflexPO40 Polyethilenic resin for waxy, full and dippy handle by foulard applications.

RolflexWF Aliphatic waterborne Nano-PU dispersion used as extender for wrinkle free treatments.

In an embodiment, the invention provides an article comprising a fiber or yam 10 having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is traditionally finished with a softener.

Suitable softeners are known to those of skill in the art Exemplary, non¬ limiting examples of softeners from a representative supplier, Lamberti SPA, are given in 15 the following table.

Texamina C/FPN Cationic softening agent with a very soft handle particularly recommended for application by exhaustion for all kind of fabrics.

Suitable alsofor cone application.

Texamina C SAL flakes 100% cationic softening agent in flakesform for all type of fabrics.

Dispersible at room temperature.

Texamina CL L1Q.

Anphoteric softening agent for all types of fabrics.

Not yellowing.

Texamina HVO Anphoteric softening agent for woven and knitted fabrics of cotton, other cellulosics and blends.

Provides a soft, smooth and dry handle.

Applied by padding.

Texamina SIL Nonionic silicon dispersion in water.

Excellent softening, lubricating and anti-static properties for all fibre types by padding.

Texamina SILK Special cationic softener with silk protean inside.

Provides a “swollen touch” particularly suitable for cellulosic, wool, silk. 138 Date re?ue/Date received 2023-07-20LamfinishLW All-in compound based onspecial polymeric hydrophilic softeners; by coating, foulard, and exhaustion.

ElastolamE50 General purposemono-component silicone elastomeric softener for textile finishing.

ElastolamEC 100 Modifiedpolysiloxanemicro-emulsion whichgives a permanent finishing,with extremely soft and silky handle.

In an embodiment. the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 5 wherein the article is a fabric, and wherein the fabric is traditionally finished with a handle modifier.

Suitable handle modifiers are known to those of skill in the art.

Exemplary, non-limiting examples of handle modifiers from a representative supplier, Lamberti SEPA, are givenin the followingtable.

Poliflex CSW Cationic anti-slipping agent.

Poliflex R75 Parafine finishing agent to give waxy handle.

Poliflex s Compound specifically developed for special writingeffects.

Poliflexm Compound for special dry-waxy handle.

Lamsoft SW 24 Compound for special slippy handle specifically developed for coating application.

Lamfinish SLIPPY All-in one compoundto get a slippy touch; by coating.

LamfinishGUMMY All-in-one compoundto get a gummy touch;by coating.

LamfinishOLDRY All-in-one compoundto get dry-sandy touch especially suitable for vintage effects;by coating. 10 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coaling comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is traditionally finished with a waterbornepolyurethane (PU) dispersion.

Suitable waterborne polyuretihane dispersions 15 for traditional finishing are known to those of skill in the art.

Exemplary, non-limiting examples of waterborne polyuretihane dispersions for traditional finishing from a representative supplier,Lamberti SPA, aregivenin the followingtable. 139 Bate ragoa/Bate receivedRolflex LB 2 Aliphatic waterbornePU dispersion particularly suggested for the formulation of textile coatings wherebright andrigidtop finish isrequired.Itisparticularly suitable as a finishing agent for organza touch on silk fabrics.

Transparent and shiny.

Rolflex HP 51 Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for outwear,luggage, technical articles especially wherehardand flexible touchis required.

Transparent and shiny RolflexPU879 Aliphatic waterbornePU dispersionparticularly suggested for the formulationof textile coatings for outwear, luggage, technical articles where a medium-hard and flexibletouchis required.

Rolflex ALM Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for outwear, luggage, technical articles where a soft and flexible touch is required.

Canbe also suitable for printingapplication.

Rolflex AP Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for outwear, fashion where a soft and gummy touch isrequired.

Rolflex W4 Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for clothing, outwear where a full, soft andnon sticky touchisrequired.

Rolflex ZB7 Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for clothing, outwear, sportswear, fashion and technical articles forindustrial applications.

The product has a very high charge digestion properties, electrolytes stability and excellent mechanical and tear resistance.

Canbe also suitable for foamcoatingand printingapplication.

Rolflex BZ 78 Aliphatic waterbornePU dispersion particularly suggested for the formulation of textile coatings for clothing, outwear, sportswear, fashion and technical articles for industrial applications.

The product has anexcellent hydrolysis resistance, a very high charge digestionand electrolytes stability and anexcellent mechanical andtear resistance.

Can be also suitable for foam coatingand printingapplication.

RolflexKUO Gives tothe coated fabric afull, soft, and slightly sticky handle with excellent fastness onall types of fabrics.

Rolflex OP 80 Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for outwear, luggage and fashion finishes where an opaquenon writingeffectis desired. 140 Date re?ue/Date received 2023-07-20Rdflex NBC Aliphatic waterbornePU dispersion general1y usedby padding applicationas a filling and zero formaldehyde sizing agent.

Can beused for outwear and fashion finishingwhere a full, elastic andnon-sticky touchisrequired.

RolflexPAD Aliphatic waterbornePU dispersion specifically designed for padding application for outwear, sportswear and fashion applications where a full, elastic and non sticky touchis required.Excellent washinganddry cleaning fastness as well as goodbath stability.

RolflexPN Aliphatic waterbornePUdispersion generally appliedby padding application for outerwear and fashionhigh quality applications where strong, elastic non sticky finishes are required.

ElafixPV4 Aliphaticblocked isocyanatenano-dispersionusedinorder to give anti-felting and anti-pilingproperties topure wool fabrics and hisblend.

Rdflex SW3 Aliphatic waterbornePU dispersion particularly suggestedto beusedby paddingapplication for the finishing of outwear, sportswear andfashion where a slippery and elastic touchis required.It is also a good anti-pilling agent.

Excellentin wool application.

RolflexC86 Aliphatic cationic waterbornePUdispersion particularly suggested for the formulation oftextile coatings for dothing, outwear, fashion where medium-soft andpleasant fulltouchis required.Fabrics treated withtheproduct canbe dyedwitha selection of dyes, to get double-color effects of different intensity.

RdflexCN29 Aliphatic cationic waterbornePUdispersion particularly suggested for the formulation oftextile coatings for dothing, outwear, fashion where soft andpleasantfull touchis required.Fabrics treated with theproduct canbe dyedwitha selection of dyes, to get double-color effects of different intensity.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 5 wherein the article is a fabric, and wherein the fabric is traditionally finished with a finishing resin.

Suitable finishing resins are known to those of skill in the art.

Exemplaty, non-limiting examples of finishing resins from a representative supplier, Lamberti SPA, are givenin the followingtable. 141 Date re?ue/Date received 2023-07-20Textol 110 Handle modifier with very soft handle for coating finishes TextolRGD Water emulsion of acrylic copolymer for textile coating, with very rigidhandle.

TextolSB21 Butadienic resinfor finishing andbinder for textileprinting ApprettoPV/CC Vinylacetate water dispersion forrigidstiffening AmisoloB CMS water dispersion for textile finishing as stiffening agent LamovilRP PVOHstabilized solution as stiffening agent In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 5 wherein the article is a fabric, and wherein the fabric is technically finished with a waterborne polyurethane dispersion.

Suitable waterborne polyurethane dispersions for technical finishing are known to those cf skill in the art Exemplary, non-limiting examples of waterborne polyurethane dispersions for technical finishing from a representative supplier,Lamberti SPA, are givenin the following table.

Rolflex AFP Aliphatic polyether polyurethane dispersioninwater.

The product has highhydrolysisresistance, goodbreakingload resistance andexcellent tear resistance.

Rolflex ACF Aliphatic polycarbonate polyurethane dispersionin water The product shows goodPU andPVCbonding properties, excellent abrasion resistance as well as chemical resistance, includedalcohol.

Rolflex V 13 Aliphatic polyether/acrylic copolymer polyurethane dispersion inwater.

Theproducthas goodthermoadhesiveproperties and good adhesionproperties onPVC.

Rolflex K 80 Aliphatic polyether/acrylic copolymer polyurethane dispersion inwater.ROLFLEXK 80is specifically designed asahigh performing adhesive for textilelamination.

Theproduct has excellent perchloroethylene and water fastness.

Rolflex ABC Aliphatic polyether polyurethane dispersioninwater.

Particularly, theproduct presents very high water column, excellent electrolyte resistance, highLOIindex,high resistance to multiplebending.

Rolflex ADH Aliphatic polyether polyurethane dispersion inwater.

The producthas a very high water column resistance.

Rolflex W4 Aliphatic waterbornePU dispersionparticularly suggested for the formulationof textile coatings for clothing, outwear where afull, soft andnon-sticky touchisrequired. 142 Date re?ue/Date received 2023-07-20RdflexZB7 Aliphatic waterbornePU dispersion particularly suggested for the formulationof textile coatings for clothing, outwear, sportswear, fashion and technical articles for industrial applications.

The product has a very high charge digestion properties, electrolytes stability and excellent mechanical and tearresistance.

Canbe also suitable for foam coatingand printingapplication.

RolflexBZ78 Aliphatic waterbomedPUdispersion particularly suggested forthe formulation oftextile coatings for clothing, outwear, sportswear, fashion and technical articles forindustrial applications.

The product has an excellent hydrolysis resistance, a very high charge digestionand electrolites stability and anexcellent mechanical andtear resistance.

Can be also suitable for foam coatingand printingapplication.

RolflexPU147 Aliphatic polyether polyurethane dispersioninwater.

This product showsgoodfilm formingproperties atroom temperature, fthas high fastness tolight and ultraviolet radiation andgood resistance to water, solvent and chemical agents, as well as mechanical resistance.

RolflexSG Aliphatic polyether polyurethane dispersioninwater.Due to its thermoplastic propertiesitis suggestedto formulateheat activated adhesives atlow temperatures.

ElafixPV4 Aliphaticblockedisocyanatenano-dispersionusedinorder to give antifeltingand antipilling propertiesto pure wool fabrics andhis blend.

Rolflex C 86 Aliphatic cationic waterbornePUdispersion particularly suggested for the formulation oftextile coatings for clothing, outwear,fashionwhere medium-soft andpleasant full touchis required.Fabrics treated withtheproduct canbe dyedwitha selectionof dyes, toget double-color effects of different intensity.

RolflexCN29 Aliphatic cationic waterbornePUdispersion particularly suggested for the formulation oftextile coatings for clothing, outwear, fashionwhere soft andpleasant full touchis required.Fabrics treated withtheproduct canbe dyedwith a selection of dyes, to get double-color effects of different intensity.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 5 wherein tire articleis a fabric, and whereinthe fabric istechnically finished with an oil or waterrepdlant.

Suitable oil or water repellants for technical finishing areknownto those 143 Date re?ue/Date received 2023-07-20of skill in the art.

Exemplary, non-limiting examples of oil or water repdlants for technical finishing from a representative supplier, Lamberti SPA, are given in the following table.

LamgardFT 60 General purpose fluorocarbon resan for water and oil repellency; by padding application.

Lamgard48 High performance fluorocarbon resin for water and oil repellency; by padding application.

High rubbing fastness.

ImbitexNRW3 Wetting agent for water-and oil repellent finishing.

LamgardEXT Crosslinker for fluorocarbon resins to improve washing fastness. 5 In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is technically finished with a flame retardant.

Suitable flame retardants for technical finishing are known to those of skill in 10 the art.

Exemplary, non-limiting examples of flame retardants for technical finishing from a representative supplier, Lamberti SPA, are given in the following table.

Piroflam 712 Hon-permanent flame retardant compound for padding and spray application.

Piroflam ECO Alogen free flame retardant compound for back coating application for all kind of fibers.

Piroflam UBC Flame retardant compound for back coating application for all kind of fibers.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof 15 having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the article is a fabric, and wherein the fabric is technically finished with a crosslinker.

Suitable crosslinkers for technical finishing are known to those of skill in the art.

Exemplary, non-limiting examples of crosslinkers for technical finishing from a representative supplier, Lamberti SPA, are given in the following table. 144 Rolflex BK8 Aromatic blocked polyisocyanate in water dispersion.

It is suggested as a cross-linking agent in coating pastes based of Date re?ue/Date received 2023-07-20polyurethane resins to improve washi ng fastness.

Fissativo05 Water dispersible aliphatic polyisocyanate suitable as crosslinking agent for acrylic and polyurethane dispersions to improve adhesion and wet and dry scrub resistance.

Resina MEL Melammine-formaldheyde resin.

Cellofix VLF Low formaldheyde malammine retin.

In an embodiment, the invention provides an article comprising a fiber or yam having a coating, wherein the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, 5 wherein the article is a fabric, and wherein the fabric is technically finished with a thickenerfor technical finishing.

Suitable thickeners for technical finishing are known to those of skill in the art Exemplary, non-limiting examples of thickeners for technical finishing from a representative supplier, Lamberti SPA, are given in the following table.

Lambicol CL 60 Fully neutralised synthetic thickener for pigment printing in oil/water emulsion; medium viscosity type ViscolamPL cone.

Nonionic polyurethane based thickener with pseudoplastic behavior.

Viscolam 115 new Acrylic thickener; not neutralised.

Viscolam PS 202 Nonionic polyurethane based thickener with newtonian behavior.

Viscolam 1022 Nonionic polyurethane based thickener with moderate pseudoplastic behavior. 10 In any of the foregoing textile or leather embodiments, the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa.

In any of the foregoing textile or leather embodiments, the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 6 kDa to about 16 kDa.

In any of the foregoing tortile 15 or leather embodiments, the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 17 kDa to about 38 kDa.

In any of the foregoing textile or leather embodiments, the coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 39 kDa toabout80 kDa. 145 Date re?ue/Date received 2023-07-205 Date re?ue/Date received 2023-07-20 In any of the foregoing textile or leather embodiments, the silk based proteins or protein fragments thereof have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 toabout 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating die fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for at least 10 days.

OtherMaterials Coated with Silk Fibroin-Based Protein Fragments In an embodiment, the invention provides a material coated with silk fibroinbased proteins or fragments thereof.

Die material may be any material suitable for coating, including plastics (e.g., vinyl), foams (e.g., for use in padding and cushioning), and various natural or synthetic products.

In an embodiment, the invention provides an automobile component coated with silk fibroin-based proteins car fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa.

In an embodiment, the invention provides an automobile component coated with silk fibroin-based proteins or fragments thereof having a weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispereity of between about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for at least 10 days.

In an embodiment, the invention provides an automobile component coated with silk fibroin-based proteins or fragments thereof, wherein the automobile component exhibits an improved property relative to an uncoated automobile component.

In an embodiment, the invention provides an automobile component coated with silk fibroin-based proteins or fragments thereof, wherein the automobile component exhibits an improved property relative toan uncoated automobile component, and wherein the automobile component is 1465 Date re?ue/Date received 2023-07-20 selected from the group consisting of anupholster, fabric, aheadliner, a seat, aheadrest, a transmission control, a floor mat, a carpet fabric, a dashboard, a steering wheel, aHim, a wiringharness, an airbag cover, an airbag, a sunvisor, a seatbelt, a headrest, an armrest, and a children’s car seat, hi an embodiment, the invention provides an electrical component insulated with a coating comprising silk fibroin-based proteins or fragments thereof.

Inan embodiment, the invention provides a foam coated with silk fibroin-based proteins or fragments thereofhaving a weight average molecular weight range of about 5 kDato about 144kDa. hi anembodiment, the invention provides afoam coatedwith silk fibroin-based proteins or fragments thereof having a weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to abort 16 kDa, about 17kDa to abort 38 kDa, about 39kDa to abort 80kDa, about 60 to about 100 kDa, and about 80 kDa to abort 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and optionally wherein the protons or protein fragments, prior to coaling the fabric, do not spontaneously or gradually gelate and donot visibly change in color or turbidity when in a solution for at least 10 days.

In an embodiment, the invention provides a foam coated with silk fibroin-based proteins or fragments thereof, wherein the foam exhibits an improved properly relative to an uncoated foam, and wherein the foamis selected from the groupconsisting ofapolyurethane foam, an ethylene-vinyl acetete copolymer foam, a low density polyethylene foam, a low density polyethylene foam, a high density polyethylene foam, a polypropylene copolymer foam, a linear low density polyethylene foam, a natural rubber foam, alatex foam,and combinationsthereof.

Inany of the foregoing embodiments, the material coating comprises silk based proteins or fragments thereofhaving a weight average molecular weight range of about 5 kDa to about 144 kDa In any of the foregoing embodiments, the material coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 6 kDa to about 16 kDa.

In any of the foregoing embodiments, the material coating comprises silk based proteins or fragments thereof having a weight average molecular weight range of about 17 kDa to about 38 kDa.

In any of the foiegoing embodiments, the material coating comprises silk based proteins or fragments 1475 Date re?ue/Date received 2023-07-20 thereof having a weight axerage molecular weight range of about 39 kDa to about 80 kDa.

In any of the foregoing embodiments, the silk based proteins or protein fragments thereof have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa. about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and wherein the proteins or protein fragments, priorto coating die fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for atleast 10 days.

Processesfor Coating Textiles,and.Leathers with, Silk Fibroin-Based Protein Fragments, In an embodiment, a method for silk coating a textile, leather, or other material (such as a foam) includes immersion of the textile, leather, or other material in any of the aqueous solutions of pure silk fibroin-based protein fragments of the present disclosure In an embodiment, a method for coating a textile, leather, or other material (such as a foam) indudes spraying.

In an embodiment, a method for coating a textile, leather, or other material (such as a foam) indudes chemical vapor deposition.

In an embodiment, a method for silk coating a textile, leather, or other material (such as a foam) includes electrochemical coating.

In an embodiment, a method for silk coating a textile, leather, <x other material (such as a foam) includes knife coating to spread any of the aqueous solutions of pure silk fibroin-based protein fragments of the present disclosure onto the fobric.

The coated article may then be air dried, dried under heat/air flow, or cross-linked to the fabric surface.

In an embodiment, a drying process includes curing with additives, inradition (e.g., using UV light), heat (e.g., microwave or radiofrequency irradiation), and/or drying at ambient condition.

In an embodiment, the invention provides a method of coating a textile, leather, or other material (such as a foam) comprising the step of applying a coating, wherein the coating comprises a solution of silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa, wherein the coating is applied to at 1east one side of the textile, leather, or other material using a method selected from the group consisting of a bath coating process, a spray coating process, a stencil (i.e., screen) process, a silk-foam 1485 Date re?ue/Date received 2023-07-20 based process, a roller-based process, a magnetic roller process, a knife process, a transfer process, a foam process, a lacquering process, a supercritical fluid impregnation process, and a printing process.

In an embodiment, the invention provides a method of coating a textile or leather comprising a step selected from the group consisting of providing an unwinding device used to unroll the fabric supply in a roll configuration, providing a feeding system used to control the feed rate of fabric, providing a material compensator used to maintain consistent1he fabric tension, providing a coating machine to apply the silk solution (i.e., silk fibroin-based protein fragments) in different state (liquid or foam) to the fabric, providing a measuring system used to control the amount of silk solution applied, providing a dryer used to cure or dry the silk solution on the fabric, providing a cooling station used to bring the fabric temperature close to room value, providing a steering frame used to guide the fabric to the rewinding device and maintain straight edges, providing a rewinding step used to collect the coated fabric in roll, providing UV irradiation for curing of silk and/or other fabric additives (eg., in a chemical cross¬ linking step), providing radiofrequency (RF) irradiation (e.g., using microwave irradiation) for drying and chemical cross-linking, and combinations thereof.

Chemical and enzymatic cross-linking steps suitable for use with the compositions, articles, and methods of the invention include any method known to those of skill in the art, including but not limited to N-hydroxysuccinimide ester crosslinking, imidoester crosslinking, carbodiimide crosslinking, dicyclohexyl carbodiimide crosslinking, maleimide crosslinking, haloacetyl crosslinking, pyridyl disulfide crosslinking, hydraride crosslinking, alkoxyamine crosslinking, reductive amination crossling, aryl azide crosslinking, diazirine crosslinking, azide-phosphine crosslinking, transferase crosslinking, hydrolase crosslinking, transglutaminase crosslinking, peptidase crosslinking (e.g., sortase SrtA from Staphylococcus aureus), oxidoreductase crosslinking, tyrosinase crosslinking, laccase crosslinking, peroxidase crosslinking (e.g., horseradish peroxidase), lysyl oxidase crosslinking, and combinations thereof In an embodiment, the invention provides a method of coating a textile or leather comprising the step of applying a coating, wherein the coating comprises a solution of silk based proteins or fragments thereof having a weight average molecular weight range 1495 Date re?ue/Date received 2023-07-20 of about 5 kDa to about 144 kDa, and wherein the coatingis applied to at least one side of the textile orleatherusing a supercritical fluidimpregnation process.

The supercritical fluid impregnation process may use CQz as the supercritical fluid to solubilize and impregnate silk based proteins or fragments thereof into a textile or leather, wherein the supercritical COj may include optional organic modifiers known in the art (e.g,, methanol)and may further include additional agents described herein, such as dyes.

Inan embodiment, theinventionprovides a method of coating a textile or leather comprising the step of applying a coating, wherein the coating comprises a solution of silk basedproteins or fragments thereofhavinga weight average molecular weight range of about 5 kDa to about 144 kDa, using a handheld aerosol spray suitable for consumer use or an aerosol spray system suitable for use by a professional cleaner (e.g,, a dry cleaner).

Inan embodiment, theinventionprovides a method of coating a textile or leather comprising the step of applying a coating, wherein the coating comprises a solution of silk basedproteins or fragments thereofhaving a weight average molecular weight range of about 5kDa to about 144kDa, using ahome washingmachine.

Inanembodiment, theinventionprovides a methodofcoating afabric comprising the steps of: (a) applying a pretreatment selected from the group consisting of a wetting agent, a detergent, a sequestering or dispersing agent, an enzyme, a bleaching agent, an antifoaming agent, an anti-creasing agent, a dye dispersing agent, a dye leveling agent, a dye fixing agent, a dye special resin agent, a dye anti-reducing agent, a pigment dye system anti-migrating agent, a pigment dye system binder, a delave agent, a wrinkle free treatment, a softener, a handle modifier, a waterborne polyurethane dispersion, a finishingresin, an oil or water repcllant, a flame retardant, a crosslinker, a thickener for technical finishing, or any combination thereof; (b) applying a coating comprising a solution of silk based proteins or fragments thereof having a weight average molecular weight range of about 5 kDa to about 144 kDa,usinga spray, screen, or stencil coating process; and 1505 Date re?ue/Date received 2023-07-20 (c)drying and optionally curing the coaling.

In any of the foregoing embodiments of methods, the silk based proteins or protean fragments thereof may have an average weight average molecular weight range selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 toabout 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk based proteins or fragments thereof have a polydispersity of between about 1.5 and about 3.0, and optionally wherein the proteins or protein fragments, prior to coating the fabric, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for at least 10 days.

Additivesfor SilkFilmDrBfl^.P^ and Solutions.Therepf In an embodiment, a solution of the present disclosure is contacted with an additive, such as a therapeutic agent and/or a molecule.

In an embodiment, molecules include, but are not limited to, antioxidants and enzymes Tn an embodiment, molecules include, tat are not limited to, ceramics, ceramic partides, metals, metal partides, polymer particles, aldehydes, luminescent molecules, phosphorescent molecules, fluorescent molecules, inorganic partides, organic particles, selenium, ubiquinone derivatives, thiol-based antioxidants, saccharide-containing antioxidants, polyphenols, botanical extracts, caffeic add, apigenin, pvcnogenol, resveratrol, folic acid, vitamin B12, vitamin B6, vitamin B3, vitamin E, vitamin C and derivatives thereof, vitamin D, vitamin A, astaxathin, Lutein, lycopene, essential fatty acids (omegas 3 and 6), iron, zinc, magnesium, flavonoids (soy, Curcumin, Silymarin, Pycnongeol), growth factors, aloe, hyaluronic acid, extracellular matrix proteins, cells, nucleic acids, biomarkers, biological reagents, zinc oxide, benzyol peroxide, retnoids, titanium, allergens in a known dose (for sensitization treatment), essential oils including, but not limited to, lemongrass or rosemary oil, and fragrances.

Therapeutic agents indude, but are not limited to, small molecules, drugs, proteins, peptides and nucleic acids.

In an embodiment, a solution of the present disclosure is contacted with an allergen of known quantity prior to forming the article.

Allergens include but are not limited to milk, eggs, peanuts, tree nuts, fish, shellfish, soy and wheat.

Known doses of allergen loaded within a silk artide can be 1515 Date re?ue/Date received 2023-07-20 released at a known rate for controlled exposure allergy study, tests and sensitization treatment Inan embodiment, silk fibroin-based protein fragments and solutions thereofmay be combined with other soluble andinsoluble additives coated ontotextiles andleather as describedherein, wherein the silk fibroin-basedprotein fragments and solutions functions as a binder or a dispersion medium for the additives.

Additives described herein and those known of ordinary skill in the art for use with coating textiles and leather may be used.

Th® combinations of silk fibroin-based protein fragments and solutions thereof with other soluble and insoluble additives may exhibit improvedproperties as described herein.

Theproperty that isimproved may be selected from the group consisting of color retention, resistance to microbial growth, resistance to bacterial growth, resistance to fungal growth, resistance to the buildup of static electrical charge, resistance to the growth ofmildew, transparency of the coating, resistance to freeze-thaw cycle damage, resistance from abrasion, blocking of ultraviolet (UV) radiation, regulation of the body temperature of a wearer, resistance to tearing, elasticity of the article, rebound dampening, tendency to cause itching in the wearer, thermal insulation of the wearer, wrinkle resistance, stain resistance, stickiness to skin, flameresistance, and combinations thereof.

For example, silk fibroin-based protein fragments and solutions thereof may be combinedwithinsoluble ceramic particles as a suspension, and subsequently coated onto a textile using airy of the methods described herein to provide furflier thermal insulation for the wearer and/or to provide improved flame resistance, or to provide other improved properties.

Inan embodiment, a solution of the present disclosure is used to create an article withmicroneedlesby standardmethods knownto onein the art for controlled delivery of moleculesor therapeutic agents to or through the skin.

Asused herein, the term “fibroin” includes silkworm fibroin and insect or spider silk protein.

In an embodiment, fibroin is obtained from Bombyx mart.

In an embodiment, the spider silk proteinis selected from the group consisting of swathing silk (Achnifonn gland silk), egg sac silk (Cylindriform gland silk), egg case silk (Tubuliform 1525 Date re?ue/Date received 2023-07-20 silk), nan-sticky dragline silk (Ampullate gland silk), attaching thread silk (Pyriform gland silk), stidsy silk core fibers (FlageHiform gland silk), and sticky silk outer fibers (Aggregate gland silk). 1 is a flow chart showing various embodiments for producing pure silk fibroin-based protein fragments (SPFs) of the present disclosure.

It should be understood that not all of the stepsillustrated are necessarily required to fabricate all silk solutions of thepresort disclosure.

As illustratedin Fig. l,step A,cocoons (heat-treated or non-heattreated), silk fibers, silk powder or spider silk can be used as the silk source.If starting from raw silk cocoons fromBombyx mart, the cocoons canbe cut into small pieces, for example pieces of approximately equal si®, step Bl.

The raw silk is then extracted and rinsed toremove any sericin, step Cla This results in substantially sericin freeraw silk.

In an embodiment, water is heated to a temperature between 84T and 100°C (ideally boiling) and then NajCOj (sodium carbonate) is added to the boiling water until the NajCOj is completely dissolved.

The raw silk is added to the boiling water/NajjCOj (1(MFC) and submerged for approximately 15-90 minutes, where boiling for a longer time results in smaller silk protein fragments.

In an embodiment, the water volume equals about 0.4 x raw silk weight and theNaiCOs volume equals about. 0.848 x raw silk weight.

In an embodiment, the water volume equals 0.1 x raw silk weight and the Na2CO3 volumeis maintainedat 2.12g/L.

Thisis demonstrated in Fig. 38A andFig. 38B silk mass (x-axis) was variedin the same volume of extraction solution (i.e., Ihe same volume of water and concentration of NazCO?) achieving sericin removal (substantially sericin free) as demonstrated by an ovendl silk mass loss of 26 to 31 percent (y-axis).

Subsequently, the water dissolvedNa^COj solution is drained and excess watesWajCOa is removed from the silk fibroin fibers (e.g., ring out the fibroin extract by hand, spin cyde using a machine, etc ).

The resulting silk fibroin extract is rinsed with warm tohot water to remove any remaining adsorbed sericin or contaminate, typically at a temperature range of about 40°C to about 80°C, changing the volume of water at least once (repeated for as many times as required).

The resulting silk fibroin extract is a substantially sericin-depleted silk fibroin.

In an embodiment, the resulting silk fibroin extract is rinsed with water at a temperature of about 60T.

In an embodiment, the volume of rinse water for each cycle equals 0.1 L to 0.2 L x raw silk weight.It may be 1535 Date re?ue/Date received 2023-07-20 advantageous to agitate, turn or circulate the rinse water to maximize the rinse effect.

Atarinring, excess waterisremoved tanthe extracted silk fibroinfibers(e.g,ringout fibroin extract by hand or using amachine).

Alternatively, methodsknown to one skilled indie art such aspressure, temperature, or other reagents or combinationsthereofmay be usedfor toe purpose of sericin extraction.

Altemativdy, the silk gland (100% sericin free silk protein) can be removed directly from a worm.

This would result in liquid silk protan, without any alterationoftheprotein structure, free of sericin.

The extracted fibroin fibers are then allowed to dry completely.

Fig. 3 is a photograph showing dry extracted silk fibroin.

Once dry, the extracted silk fibroin is dissolvedusinga solvent added to the silk fibroin at a temperature between ambient and boiling, step Clb.

Inan embodiment, the solvent is a solution ofLithium bromide (LiBr) (boiling for LiBr is 140°C).

Alternatively, the extracted fibroin fibers are not. dried but wet andplacedin the solvent; solvent concentration can thenbe varied to achieve similar concentrations as to when adding dried silk to the solvent The final concentration of LiBr solvent can rangefrom0.1Mto 9.3M.

Fig. 39 is a table summarizingtheMolecular Weights of silk dissolved from different concentrations of Lithium Bromide (LiBr) and from different extraction and dissolution sizes.

Complete dissolution of the extracted fibroin fibers canbe achieved by varying the treatment time and temperature along with the concentration of dissolving solvent.

Other solvents may be used including, but not limited to,phosphatephosphoric add, caldum nitrate, calcium chloride solution or other concentrated aqueous solutions of inorganic salts.

To ensure complete dissolution, the silk fibers should be fully immersed within the already heated solvent solution and then maintained at atemperatureranging from about 60°C to about 140°C for 1-168 hrs.

In an embodiment, the silk fibers shouldbe fully immersed within the solvent solutionand then placedinto a dry ovenat a temperature ofabout 100°C for about 1hair.

The temperature at which the silk fibroin extract is added to theLiBr solution (or vice versa) has an effect on the time required to completely dissolve the fibroin and on theresulting molecular wei^it andpolydispersity of the final SPF mixture solution, hi an embodiment, silk solvent solution concentration is less than or equal to 20%wh.

In addition, agitation during introduction or dissolutionmay beusedto facilitate dissolution at varying temperatures and concentrations.

Hie temperature of toe LiBr solution will 1545 Date re?ue/Date received 2023-07-20 provide control over the silk protean fragment mixture molecular weight and pctydispsaty created.

In an embodiment, a higher temperature will more quiddy dissolve die silk offering enhanced process sealability and mass production of silk solution.Inan embodiment.using aLiBr solutionheated to a temperaturebetween 80°C - 140°C reduces the time required in an oven in order to achieve full dissolution.

Varying time and temperature at or above 60°C of the dissolution solvent will alter and control the MW andpolydispersity of die SPFmixture solutions famed from the originalmolecular weight ofthe native silk fibroin protein.

Alternatively, whole cocoons may be placed directly into a solvent, such as LiBr, bypassing extraction, step B2.

This requires subsequent filtration of silk worm particles from the silk and solvent solution and sericin removal usingmethods know inthe art for separating hydrophobic and hydrophilic proteins such as a column separation and/or chromatography, ion exchange, chemical precipitation with salt and/or pH, and or enzymatic digestion and filtration or extraction, all methods are common examples and without limitation for standard protein separation methods* step O.

Non-heat treated cocoons with the silkworm removed, may alternatively be placedinto a solvent such as LiBr, bypassing extraction.

The methods described above may be used for sericin separation, with the advantage that non-heat treated cocoons will contain significantly less worm debris.

Dialysis may be used to remove the dissolution solvent from the resulting dissolved fibroinprotein fragment solutionby dialyzing the solution against a volume of water, step El.

Pre-filtration prior to dialysis is helpfill to remove any debris 0.e., silk worm remnants) fromthe silk andLiBr solution, stepD.

Inone example, a 3 pm or 5pm filter is used with a flow-rate of 200-300tnL/min to filter a 0.1% to 1.0% slk-UBr solution prior to dialysis and potential concentration if desired. A method disclosed herein, as described above, is to use time and/or temperature to decrease the concentration from 93M LiBr to a range from 0.1M to 9.3M to facilitate filtration and downstream dialysis, particularly when considering creating a scalable process method.

Alternatively, without the use of additional time or temperate, a 9.3M LiBr-siJk protein fragment solution may be diluted with water to facilitate debris filtration and dialysis.

The result of dissolution at the desired time and temperate filtration is a translucent 1555 Date regue/Date received 2023-07-20 particle-free room temperature shelf-stable silk protein fragment-LiBr solution of a known MW andpolydispersity.

Itis advantageous to change die dialysis waterregulariy until the solvent has been removed (e.g., change water after 1 hour, 4 hours, and then every 12 hours for a total of 6 water changes).

The total number of water volume changes may be varied based on the resulting concentration of solvent used for silk protein dissolution and fragmentation.

After dialysis, the final silk solutionmaybe further filteredtoremove any remaining debris (i.e., silk wormremnants).

Alternatively, Tangential Flow Filtration (TFF), which is a rapid and efficient method for the separation and purification of biomolecules, may be used to remove the solvent from the resulting dissolved fibroin solution, step E2. TFF offers a highly pure aqueous silk protein fragment solution and enables scalability of the process in order to producelarge volumes of the solutionin a controlled andrepeatablemanner.

The silk and LiBr solution may be diluted prior to TFF (20% down to 0.1% silk in either water or LiBr).

Pro-filtration as desaribed above prior to TFF processing may maintain filter efficiency and potentially avoids the creation of silk gel boundary layers on the filter’s surface astiie result of the presence of debris particles.

Pre-filtration prior to TFF is also helpful toremove any remaining debris (i.e., silk worm remnants) from the silk andLiBr solution that may cause spontaneous or long-term gelation of the resulting water only solution, step D. TFF,recirculatingor single pass, may be used for the creation of water¬ silk protein fragment solutions ranging from 0.1% silk to 3O.Q% silk (more preferably, 0.1% - 6.0% silk).

Different cutoff size TFF membranes may be requiredbasedupon the desired concentration, molecular weight and polydispersity of the silk protein fragment mixturein solution.

Membranes ranging from 1-100 kDa may be necessary for varying molecular weight silk solutions created for example by varying the length of extraction boil time or the time andtemperatein dissolution solvent (e.g., LiBr).Inan embodiment, a TFF 5 or 10 kDa membraneis used to purify the silk protean fragment mixture solution and to create the final desired silk-to-water ratio.

As well, TFF single pass, TFF, and other methods known in the art, such as a felling film evaporator, may be used to concentrate the solution following removal of the dissolution solvent (e.g., LiBr) (with resulting desired concentration ranging from 0.1% to 30% silk).

This can be used as an alternative to standard HFIP concentration methods known in the art to create a water- 1565 Date re?ue/Date received 2023-07-20 based solution. A larger pore membrane could also be utilized to filter out small silk prottin fragments and to create a solution of higher molecular weight silk wifli and/or without tighter polydispersity values.

Fig. 37 is a table summarizing Molecular Weights for some embodiments of silk protein solutions of die present disclosure.

Silk protein solution processing conditions were as follows: KXFC extraction for 20 min, room temperature rinse, LiBr in 60°C oven for 4-6 hours.

Figs. 40-49 further demonstrate manipulation of extraction time, LiBr dissolution conditions, and TFF processtag and resultant example molecular weights and polydispersities, d hese examples are not intended to be limiting, but rather to demonstrate the potential of specifying parameters for specific molecular weight silk fragment solutions.

An assay for LiBr and NazCOj detection was performed using an HPLC system equipped with evaporative light scattering detector (ELSD).

The calculation was performedby linear regression of the resulting peak areas for the analyte plotted against concentration, More than one sample of a number of formulations of the present disclosure was used for sample preparation and analysis.

Generally, four samples of different formulations were weighed directly in a 10 mL volumetric flask.

Hie analytical method developed for the quantitation of NajCCh andLiBr in silk protein formulations was found to be linear in the range 10 - 165 pg/mL, withRSD for injection precision as 2% and 1% for area and 0.38% and 0.19% for retention time for sodium carbonate and lithium bromide respectively.

The analytical method can be applied for the quantitative determination of sodium carbonate and lithium bromide in silk protein formulations.

Hie final silk protein fragment solution, as shown in Fig. 4, is pure silk protein fragments and water with PPM to undetectable levels of particulate debris and/or process CMUaminants, including LiBr and NaiCOj.

Fig. 34 and Fig. 35 are tables summarizing LiBr and Na^CO? concentrations in solutions of the present disclosure.

In Fig. 34, the processing conditions included 100°C extraction for 60 min, 60°C rinse, 100°C LiBr in 100°C oven for 60 min. TFF conditions including pressure differential and number of diafiltration volumes were varied.

In Fig. 35, the processing conditions included 100°C bad for 60min, 60°C rinse,LiBrin 60°C oven for 4-6 hours.

In an embodiment, a SPF 1575 Date re?ue/Date received 2023-07-20 composition of the present disclosure is not soluble in an aqueous solution due to the crystallinity of die protein.

In an embodiment, a SPF composition of the present disclosure is soluble in an aqueous solution.

In an embodiment, the SPFs of a composition ofIhe present disclosureinclude a <srystalline portion ofabouttwo-tods and an amorphous region of about one-third.

In an embodiment, the SPFs of a composition ofthe present disclosure include a crystalline portion of about or.e-lralf and an amorphous re^ai of about one-half In an embodiment, the SPFs of a composition of the present disclosure include a 99% crystalline portion and a 1% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 95% crystalline portion and a 5% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 90% crystalline portion and a 10% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 85% aystalline portion and a 15% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 80% crystalline portion and a 20% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 75% crystalline portion and a 25% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 70% oystalline potion and a 30% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 65% crystalline portion and a 35% amorphous re^on. fa an embodiment, the SPFs of a composition of the present disclosure include a 60% crystalline portion and a 40% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 50% crystalline portion and a 50% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 40% crystalline portion and a 60% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 35% crystalline portion and a 65% amorphous region, fa an embodiment, the SPFs of a composition of the present disclosure include a 30% oystalline portion and a 70% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 25% crystalline portion and a 75% amorphous region, fa an embodiment, the SPFs of a composition of tire present disclosure include a 20% crystalline portion and a 80% 1585 Date re$ue/Date received 2023-07-20 amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 15% aystaffing portion and a 85% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 10% crystalline portion and a 90% amorphous region.

In an embodiment, the SPFs of a composition of the present disclosure include a 5% crystallin© portion and a 90% amorphous region.

In an embodiment, the SPFs rtf a composition of the present disclosureinclude a 1% crystallineportionanda 99%amorphous region.

A unique feature of toe SPF compositions of the present disclosure are shelf stability (they will not slowly or spontaneously gel when stored in an aqueous solution and thereis no aggregation of fragments and therefore no increase in molecular weight over time), from 10 days to 3 years depending on storage conditions, percent silk, and number of shipments and shipment conditions.

Additionally pHmay be alteredtoextend shelf-life and/or support shipping conditions by preventing premature folding and aggregation of the silk.

In an embodiment, a SPF solution composition of the present disclosure has a shelf stability for up to 2 weeks at room temperature (RT).

In an embodiment, a SPF solutioncompositionof the present disclosurehas a shelf stability for up to 4 weeks at RT.

In an embodiment, a SPF solution composition of the present disclosure has a shelf stability for up to 6 weeks at RT.

In an embodiment, a SPF solution composition of the present disclosure has a shelf stability for up to 8 weeks at RT.

In an embodiment, a SPF solution composition of the present disclosure has a shelf stability for up to 10 weeks at RT.In an embodiment, a SPF solution composition of the present disclosurehas a shelfstability forup to 12 weeks atRT.

Inan embodiment, a SPF solution composition of the present disclosure, has a shelf stability ranging from about 4 weeks to about 52 weeks at RT.

Table 1 below shows shelf stability test results for embodiments of SPF compositions of thepresent disclosure. 159 Tablet Shelf Stability of SPF Compositions of thePresentDisclosure % Silk Temperature Time to Gelation 2 RT 4 weeks 2 4C >9 weeks 4 RT 4 weeks5 Date re?ue/Date received 2023-07-20 4 4C >9 weeks 6 RT 2 weeks 6 4C >9 weeks A silk fragment-water solution of the present disclosure can be sterilized followirg standard methodsin the art not limited to filtration, heat, radiation or e-beam.

It is anticipated that the silk protein fragment mixture, because of its shorter protein polymer length, will withstand sterilization better than intact silk protein solutions describedin the art.

Additionally, silk articles created from the SPF mixtures described herein may be sterilized as appropriate to application.

Fig. 2 is a flow chart showing various parameters that canbe modified duringthe process ofproducinga silk protein fragment solution of the present disclosure duringthe extraction and the dissolution steps.

Select method parameters may be altered to achieve distinct final solution characteristics depending upon the intended use, e.g,, molecular weight and polydisperaity.It should be understood that not all of the steps illustrated are necessarily required to fabricate all silk solutions of thepresent disclosure.

Inan embodiment, aproem for producing a silk protein fragment solution ofthe present disclosure includes forming pieces of silk cocoons from the Bombyx mori silk worm; extracting the pieces at about 100°C in a solution of water and NajCCb for about 60 minutes, wherein a volume of the water equals about 0.4 x raw silk weight and the amount ofNa^COsis about 0.848 x the weight of the pieces to form a silk fibroin extract; triple rinsing the silk fibroin extract at about WC for about 20 minutes per rinse in a volume of rinse water, wherein the rinse waler for each cycle equals aboil 0.2L x the weight ofthe pieces; removing excess water from the silk fibroin extract; drying the silk fibroin extract; dissolving thedry silk fibroin extract inaLiBr solution, whereintheLiBr solutionis first heatedto about 100°C to create a silk andLiBr solution and maintained; placing the silk and UBr solutionin a dry oven at about 100°C for about 60 minutes to achieve complete dissohition and further fragmentationof the native silk proteinstructure into mixture with desired molecular weight and polydispersity; filtering the solution to remove airy remainingdebris fromthe silkworm; dilutingthe solution with watertoresult in a 1% silk solution; and removing solvent from the solution using Tangential Flow 1605 Date re?ue/Date received 2023-07-20 Filtration (IFF).

In an embodiment, a 10 kDa membrane is utilized to purify the silk solution and create the final desired silk-to-wMer ratio. TFF can then be used to further concenttale thepure silk solution toa concentration of2% silk to water.

Each process step from raw cocoons to dialysis is scalable to increase efficiency in manufacturing.

Whole cocoons are currently purchased as the raw material, but pre¬ cleaned cocoons or non-heat treated cocoons, where worm rentoval leaves minimal debris, have also been used.

Cutting and cleaning the cocoons is a manual process, however for scalability this process could be made less labor intensive by, for exampleusingan automatedmachine incombination with compressed air to remove toe worm and any particulates, orusing a cutting mill to cut the cocoons into smaller pieces.

The extraction step, currently performedin small batches, could be completed ina larger vessel, for example an industrial washing machine where temperatures at or in between oOT to 100°C can be maintained.

The rinsing step could also be completed in toe industrial washing machine, eliminating toe manual rinse cycles.

Dissolution of toe silk in LiBr solution could occur in a vessel other than a convection oven, for example a stirred tank reactor.

Dialyzingthe silk through a series of water changesis a manual and time intensive process, which could be accelerated by changing certain parameters, for example diluting the silk solution prior to dialysis.

The dialysis process could be scaled for manufacturing by using semi-automated equipment, for example a tangential flow filtration system.

Varying extraction (i.e., time and temperature), LiBr (i.e., temperature of LiBr solution when added to silk fibroin extract or vice versa) and dissolution (i.e., time and temperature) parameters results in solvent and silk solutions with different viscosities, homogeneities, and colors (see Figs. 5-32).

Increasing the temperature for extraction, lengthening the extraction time, using a higher temperatureLiBr solution at emason and over time whendissolving the silk and increasing the time at temperature(e.g,inanoven as shown here, or an alternative heat source) all resulted in less viscous and more homogeneous solvent and silk solutions.

While almost all parameters resulted ina viable silk solution, methods that allow complete dissolution to be achievedin fewer titan 4 to 6 hours arepreferred forprocess scalability. 1615 Date re?ue/Date received 2023-07-20 Figs. 5-10 show photographs of four different silk extraction combinations tested: 90°C 30min, 90°C 60min, 100°C 30 mm, and 100°C 60 min.

Briefly, 9.3 MLiBr was prepared and allowed to sit at room temperature for at least 30 minutes. 5 mL of LiBr solution was added to 1.25 gof silk and placedin the 60°C oven, Samples from each set were removed at 4, 6, 8, 12, 24, 168 and 192 hours.

The remaining sample was photographed figs. 11-23 show photographs of four different silk extraction combinations tested: 90*0 30 min, 90°C 60 min, 100°C 30 min, and 100°C 60 min.

Briefly, 9.3 M LiBr solution was heated to one of four temperatures: 60®C, 80°C, 100°C or baling. 5 mL of hot LiBr solution was added to 1.25 g of silk and placed in the 60°C oven.

Samples from each set were removed at 1, 4 and 6 hours.

The remaining sample was photographed. figl. 24-32 show photographs of four different silk extraction combinations tested: Four different silk extraction combinations were used: 90°C 30 min, 90°C 60 min, 100“C 30 min, and 100°C 60min.

Briefly, 93MLiBr solution was heated to one offour temperatures: 60°C, 80°C, 100°C or boiling. 5 mL ofhot LiBr solution was added to 1.25 gof silk and placed in the oven at the same temperature of the LiBr.

Samples from each set were removed at 1, 4 and 6 hours. 1 mL of each sample was added to 7.5 mL of 9.3 M LiBr and refrigerated for viscosity testing.

The remaining sample was photographed.

Molecular weigh: of the silk protein fragments may be controlledbasedupon the specific parameters utilized during the extraction step, including extraction time and temperature; specific parameters utilized during tire dissolution step, including the LiBr temperature at the time of submersion ofthe silk in to the lithiumbromide and time that the solution is maintained at specific temperatures; and specific parameters utilized during the filtration step.

By controlling process parameters using the disclosedmethods, it is posable to create SPF mixture solutions with polydispersity equal to or lower than 2.5 at a variety of different molecular weight ranging from 5 kDa to 200 kDa, more preferably between 10 kDa and 80kD4.

By altering process parameters to achieve silk sedations with different molecular weights, a range of fragment mixture end products, 1625 Date re?ue/Date received 2023-07-20 with desired polydispersity of equal to or less than 2.5 may be targeted based upon the desired performance requirements.

Additionally, SPF mixture solutions with a polydispersity of greater than 2.5 can be achieved.

Further; two solutions with different average molecular weights and polydispersities can be mixed to create combination solutions.

Alternatively, a liquid silk gland (100% sericin free silk protein) that has been removeddirectly from a worm couldbe used in combinationwith any ofthe SPFmixture solutions of the present disclosure.

Molecular weight of the pine silk fibroin-based protein fragment composition was determined using High Pressure Liquid Chromatography (HPLC) with a Refractive Index Detector (RID), Polydispewity was calculatedusingCirrus GPC Online GPC/SEC Software Version 3.3 (Agilent).

Parameters were varied during the processing of raw silk cocoons into silk solution.

Varying these parameters affected die MW of tire resulting silk solution.

Parameters manipulated included (i) time and temperature cf extraction, (ii) temperature ofLiBr,(hi) temperature ofdissolution oven, and(iv) dissolution time.

Molecular weight was detenninedwithmass spec as showninFigs. 40-54.

Experiments were carried out to determine the effect of varying the extraction time.

Mp. 40-46 are graphs showing these results, and Tables 2-8 summarize the results.

Belowis a summary: A sericin extractiontime of30 minutesresultedin largerMW thana sericin extraction timeof 60minutes MW decreases withtimeinthe oven 140°CLiBr and ovenresultedinthe low endoftheconfidenceintavd to bebelowaMWof9500Da 30min extraction at the 1hour and 4 how timepointshaveundigested silk 30minextraction at the 1hour time pointresultedina significantty high molecular weight wifethelow endof the confidenceintervalbeing35,000Da Hierange ofMW reachedfor thehighend ofthe confidenceinterval was 18000 to 216000 Da(important for offering solutions with specifiedupperlimit) 163Table 2.

The effect of extraction time (30 m processed under the conditions of 100°C Bromide(LiBr) and100°C OvenDissolution in vs 60 min) on molecular weight Extraction Temperature, 100°C 1 Oven/Dissolution lime was varied ;of silk Lithium Boi! Time Oven Time Average Mw Stddev ConfidenceInterval PD 30 1 57247 12780 35093 93387 1.63 60 1 31520 1387 11633 85407 2.71 30 4 40973 2632 14268 117658 2.87 60 4 25082 1248 10520 59803 2.38 30 6 25604 1405 10252 63943 2.50 60 6 20980 1262 10073 43695 2.08 164 fable 3.The effect ofextraction time(30min vs60 min) onmolecular weight ofsilk jrocessedunder the conditionsof 100°CExtraction Temperature,balingLithium bromide(LiBr)and 60°C Oven Dissolution for 4 hr.

Sample Boil Time AverageMw Std dev Confidence Interval PD 30 min,4 hr 30 49656 4580 17306 142478 2.87 60 min,4 hr 60 30042 1536 11183 80705 2.69 Table 4.The effect ofextraction time (30min vs 60 min) onmolecular weight of silk processedunder the conditions of100°C Extraction Temperature, 60°C LithiumBromide (LiBr) and60°C OvenDissolution (Oven/DissolutionTime was varied).

Sample Boil Time Oven Time Average Mw Std dev Confidence Interval PD 30min,1hr 30 1 58436 22201 153809 2.63 60 min,1hr 60 1 31700 11931 84224 2.66 30 min,4 hr 30 4 61956.5 13337 21463 178847 2.89 60 4 hr 60 4 25578.5 2446 9979 65564 2.56 Table 5,The effect ofextraction time (30min vs 60 min) onmolecular weight of silk processedwider the conditions of 100°CExtraction Temperature, SO°C LithiumBromide (LiBr) and 80’C OvenDissolutionfor 6 hr.

Sample Bal Time Average Mw Std dev Confidence Interval PD Date re?ue/Date received 2023-07-20165 30 min,6 hr 30 63510 18693i 215775 3.40 60min,6 hr 60 25164 238 9637 65706 2.61 Table 6.The effect ofextraction time (30min vs 60 min) onmolecular weight of silk processedunder the conditions of 100°C Extraction Temperature, 80°C LithiumBromide (LiBr) and60°C OvenDissolution (Oven/Dissolution Timewas varied).

Sample Boil Time Oven Time Average Mw Std dev Confidence Interval PD 30 min,4 hr 30 4 59202 14028 190731 183760 3.10 60 min,4 hr 60 4 26312.5 637 10266i 67442 2.56 30 min,6 hr 30 6 46824 1807«i 121293 2.59 60 min,6 hr 60 6 26353 10168i 68302 2.59 Table 7.The effect ofextraction, time (30minvs 60 min) onmolecular weight ofslk processedunder the conditions of100°CExtraction Temperature, 100°C Lithium Bromide(LiBr) and 60°C OvenDissolution (Oven/Dissolution Time was varied).

Sample Boil Time Oven Time Average Mw Std dev Confidence Interval PD 30 min,4 hr 30 4 47853 19758! 115900 2.42 60min,4hr 60 4 25082 1248 1052C» 59804 2.38 30 min,6 hr 30 6 55421 8992 19153l 160366 2.89 &)min,6 hr 60 6 20980 1262 10073i 43694 2.08 Table 8.The effect ofextraction time (30minvs 60 min) onmolecular weight of silk processedunder the conditions of100°CExtraction Temperature, 140°CLithium Bromide(LiBr) and 140°C OvenDissolution (Oven/Dissolution Time was varied).

Sample Boil Time Oven Time Average Mw Std dev Confidence Interval PD 30 min,4 hr 30 4 9024.5 1102 4493 18127 2.00865 60 min,4 hr 60 4 15548 6954 34762 22358 30 min,6 hr 30 6 13021 5987 28319 2.1749 60min,6 hr 60 6 10888 5364 22100 2.0298 Date re?ue/Date received 2023-07-20Experiments were carried out to determine the effect ofvarying the extraction temperature.

Fig. 47is agmph showingtheseresults,and Table 9 summarizes1he results Belowisa summary: Sericinextraction at 90°C resultedin higherMW than sericin extraction at 5 100°C extraction - Both 90°C and100°C show decreasingMW over timeinthe oven Table 9.The effect ofextraction temperature (90°C vs. 100°C) on molecular weight of silk processedunder the conditionsof 60min.

Extraction Temperature, 100°C Lithium Bromide(LiBr) and 100°C OvenDissolution (Oven/Dissolution Time was varied).

Sample Boil Time Oven Time AverageMw Stddev ConfidenceInterval PD 90*C,4hr 60 4 37308 4204 13368 104119 2.79 100°C,4hr 60 4 25082 1248 10520 59804 238 90°C,6hr 60 6 34224 1135 12717 92100 2.69 100°C,6hr 60 6 20980 1262 10073 43694 2.08 Experiments were carried out to determine the effect of varying theLithium 10 Bromide(LiBr)temperature when addedto silk Figs. 48-49 are graphs showingthese results, and T ables 10-11 summarize theresults.

Below is a summary: - Noimpact onMW or confidenceinterval(allCI~10500-6500Da) Studiesillustrated that the temperature ofLiBr-silk dissolution, asUBris added andbegins dissolving, rapidly drops below theoriginalLiBr temperature 15 due to themajority ofthe mass being silk at room temp 166 Table 1®.

The effect ofLithium Bromide processedunder the conditions of 60min.

Temperature and60°C OvenDissolution (LiBr)temperatureon molecular weight of silk Extraction Time 100°CExtraction Oven/Dissolution Time was varied).

Sample LiBr Temp fC) Oven Time Average Mw Stddev ConfidenceInterval PD 60°CLiBr, Ihr 60 1 31700 11931 84223 2.66 100 °CLiBr, Ihr 100 1 27907 200 10735 72552 2.60 RTLiBr,4hr RT 4 29217 1082 10789 79119 2.71 Date re?ue/Date received 2023-07-2060°C LiBr, 4hr 60 4 25578 2445 9978 65564 2.56 80°CLiBr, 4hr 80 4 26312 637 10265 67441 2.56 100 °CLiBr, 4hr 100 4 27681 1729 11279 67931 2.45 BoilLiBr,4hr Boil 4 30042 1535 11183 80704 2.69 KTLiBr,6hr RT 6 26543 1893 10783 65332 2.46 »»CIiBr, 6hr 80 6 26353 10167 68301 2.59 100 °CLiBr, 6hr 100 6 27150 916 11020 66889 2.46 Tdbk IL.Hie effectofLithium Bromide(LiBr) temperature on molecular weight of silk processedunder the conditions of30min.Extraction Time, 100°CExtraction Temperature and60°C OvenDissolution(Oven/Dissolution Time was varied).

Sample LiBr Temp CC) Oven Time Average Mw Stddev ConfidenceInterval PD 60°CLiBr, 4hr 60 4 61956 13336 21463 178847 2.89 80°CLiBr, 4hr 80 4 59202 14027 19073 183760 3.10 100 °CLiBr, 4hr 100 4 47853 19757 115899 2.42 80°C LiBr. 6hr 80 6 46824 18075 121292 2.59 100 °CLiBr, 6hr 100 6 55421 8991 19152 160366 2.89 Experiments were carried out to determine the effect of varying the oven/dissohtiontemperature.

Figu 50-54 are ^aphs showingtheseresults, andTables 12-16 summarizetheresults.

Below is a summary: 5 - Oven temperature has lessof an effect on 60min extracted silk than30 rainextractedrilk, Withoutwislungtobeboundby theory,itisbelievedthatthe 30min silk is less degraded duringextraction and thereforethe oven temperature hasmore of an effect on thelargerMW,less degradedportionofthe silk.

For 60°C vs. 140®C ovenOre 30min extracted silk showeda very 10 significant effect of lower MW athigher oven temp, while 60min extracted silk had an effectbutmuchless 167 Date re?ue/Date received 2023-07-20He 140°C ovenresultedinalow endinthe confidence interval at~6000 Da Table11The effectof oven/dissolutiontemperature onmolecular weight of silk processedunder the conditions of 100°CExtraction Temperature, 30 min.Extraction Time, and 100°CLithiumBromide (LiBr)(Oven/Dissdutiffli Timewas varied).

Boil Time Oven Temp (°C) Oven Time Average Mw Stddev Confidence Interval PD 30 60 4 47853 19758 115900 2.42 30 100 4 40973 2632 14268 117658 2.87 30 60 6 55421 8992 19153 160366 2.89 30 100 6 25604 1405 10252 63943 2.50 Table IX The effectof oven/dissolutiontemperature on molecular weight of silk processedunder the conditions of100°C Extraction Temperature, 60min.Extraction Time, and 100®CLithiumBromide(LiBr)(OvenZDissolution Time was varied).

Boil Time OvenTemp CC) Oven Time Average Mw Stddev Confidence Interval PD 60 60 1 27908 200 10735 72552 2.60 60 100 1 31520 1387 11633 85407 2.71 60 60 4 27681 1730 11279 72552 2.62 60 100 4 25082 1248 10520 59803 2.38 60 60 6 27150 916 11020 66889 2.46 60 100 6 20980 1262 10073 43695 2.08 Table 14.The effectof oven/dissolution temperature onmolecular weight of silk processedundo-the conditions of 100°CExtraction Temperature, 60 min.Extraction Time, and 140°CLithiumBromide (LiBr)(OvenZDissolution Timewas varied).

Boil Time Oven TempfC) Oven Time Average Mw Std dev Confidence Interval PD 60 60 4 30042 1536 11183 80705 2.69 60 140 4 15548 7255 33322 2.14 Table 15,The effect of oven/dissolutiontemperature onmolecular weight of silk processedunder die conditions of 100°CExtraction Temperature, 30min.Extraction Time, and 140°CLithium Bromide (LiBr)(OvenZDissolution Timewas varied). 168 Date re$ue/Date received 2023-07-20Boil Time Oven Temp (°C) Oven Time Average Mu Std dev Confidence Interval PD 30 60 4 49656 4580 17306 142478 2.87 30 140 4 9025 1102 4493 18127 2.01 30 60 6 59383 11640 17641 199889 3.37 30 140 6 13021 5987 28319 2.17 Table 16.The effectof oven processedunder the conditio Time, and 80°CLithiumBn /dissolutiontemperature cmmolecular weight of silk ns of 100°CB£traction Temperature, 60min.Extraction amide(LiBr) (OvenZDissolution Time was varied).

Boil Time Oven Temp (°C) Oven Time Average Mw Std dev Confidence Interval PD 60 60 4 26313 637 10266 67442 2.56 60 80 4 30308 4293 12279 74806 2.47 60 60 6 26353 10168 68302 2.59 60 80 6 25164 238 9637 65706 2.61 In an embodiment, when producing a silk gel, an acid is used to help facilitate gelation.

In an embodiment, whenproducing a silk gel that includes a neutral or a basic 5 molecule and/or therapeutic agent, an acid can be added to facilitate gelation.

In an embodiment, when producing a silk gel, increasing the pH (making the gel more base) increases the shelf stability of the gel, In an embodiment, when producing a silk gel, increasing the pH (making the gel more basic) allows for a greater quantity of an acidic moleculetobe loaded into thegel. 10 In an embodiment, natural additives may be added to the silk gel to further stabilize additives.

For example, trace elements such as selenium or magnesium or Lmethoinine canbe used.

Further,light-block containers canbe added to further increase stability. 169 Date re?ue/Date received 2023-07-205 Date re?ue/Date received 2023-07-20 In an embodiment, the methods disclosed herein result in a solution with characteristics that canbe cratirolled during manufacturing, including, but notlimited to: MW-may be variedby changingextraction and/or dissolution time and temp (e.g.,UBr temperature), pressure, and filtration (e.g^ size exclusion chromatography); Structureremoval or cleavage of heavy or light chain of the fibroin protein polymer; Purity -■ hot water rinse temperature for improved sericin removal or filter capability for improved particulate removal that adversely affects shelf stability of the silk fragment protein mixture solution; Color - the color of the solution can be ccMitrdlled with, for example, UBr temp andtime; Viscosity; Clarity; and Stability of solution, The resultant pHofthe solution is typically about 7 and can be altered using an arid or base as appropriate to storage requirements.

In an embodiment, the above-described SPF mixture solutions may be utilized to coat at least a portionof a fabric which canbeused to createatextile.

In an embodiment, the above-described SPF mixture solutionsmay be weaved into yamthat can beused as a fabric inatextile.

Fig. 33 shows two HPLC chromatograms from samples comprising vitamin C.

The chromatogram shows peaks from (1) a chemically stabilized sample of vitamin C at ambient conditions and (2) a sample of vitamin C taken after 1 hour at ambient conditions without chemical stabilization to prevent oxidation, where degradation products are risible.

Fig. 3ti is a table summarizing the stability of vitamin C in chemically stabilized solutions. fasome embodiments, a composition ofthe present disclosure can fartherinclude skin penetration enhancers, including, but not limited to, sulfoxides (such as dimethylsulfoxide), pyrrolidones (such as 2-pyrrolidone), alcohols (such as ethanol or decanol) azones (such as laurocapram and l-dodecylazacydoheptan-2-one), surfactants (including alkyl caiboxylates and their corresponding acids such as oleic arid, fluoroalkylcarboxylates andtheir corresponding acids, alkyl sulfates, alkyl ether sulfates, docusates such as dioctyl sodium sulfosuccinate, alkyl benzene sulfonates, alkyl ether phosphates, andalkyl aryl eflier phosphates), glycols (such as propylene gjycol),terpenes 1705 Date re?ue/Date received 2023-07-20 (such as limonene, p-cymene, geraniol, farnesol, eugenol, menthol, terpineol, carveol, carvone,fenchone, andvcrbenone), and dimethyl isosortride.

Following are non-limiting examples of suitable ranges for various parameters in and for preparation of the silk solutions of the present disclosure.

The silk solutions of the present disclosure may include one or more, but not necessarily all, erf these parameters and may be prepared using various combinations of ranges of such parameters.

In an embodiment, die percent silk in the solution is less than 30%.

In an embodiment, the percent silk in the solution is less than 25%.

Tn an embodiment, the percent silk in the solution is less than 20?^.

In an embodiment, the percent silk in tile solutionisless than 19%, In an embodiment, the percent silk in the solution isless than 18%.

In an embodiment, the percent silk in the solution is less than 17%.

In an embodiment, the percent silk in the solution is less than 16% In an embodiment, the percent silk in die solution is less than 15%.

In an embodiment, the percent silk in the solutionisless than 14%, In an embodiment, the percent silk in the solution is less than 13%.

In an embodiment, the percent silk in the solution is less than 12%.

In an embodiment, tire percent silk in the solution is less than 11%.

In an embodiment, the percent silk in the solution is less than 10%.

In an embodiment, the percent silk in the solutionis less than 9%.

In an embodiment, the percent silk in the solution is less than 8%.

In an embodiment, the percent silk in the solution is less than 7%.

In an embodiment, the percent silk in the solution is less than 6%.

In an embodiment, the percent silk in the solutionis less than 5%.

In an embodiment, tire parcent silk in the solutionis less titan 4%.

In an embodiment, tire percent silk in the solution is less than 3%.

In an embodiment, tire percent silk in the solution is less than 2%.

In an embodiment, the percent silk in the solution is less than 1%.

In an embodiment, the percent silk in the solutionis less than 0.9%.

In an embodiment, the percent silk in the solutionisless titan 0.8%.

Inan embodiment, the percent silk in the solution is less than 0.7%.

In an embodiment, tire percent silk in the solution is less than 0,6%.

In an embodiment, the percent silk in the solution is less than 0.5%.

In an embodiment, the percent silk in tire solution is less than 0.4%.

In an embodiment, the percent silk in the solutionisless than 0.3%. in an embodiment, the percent silk in the solution isless than 1715 Date re?ue/Date received 2023-07-20 0.2%.

In an embodiment, the percent silk in the solution is less than 0.1%.

In an embodimert, the percent silkin the solutionis g-eater than 0.1%.

In an embodiment, the percent silkin the solution is greater than 0.2%.

In anembodiment, the percent silkin the solution is greater than 0,3%.

In an embodiment, 1he percent silk in the solution is greater than 0.4%.

In an embodiment, the percent silk in the solution is greater than 0.5%.

In an embodiment, the percent silk in the solution is greater than 0.6%.

In an embodiment, the percent silkin the solutionis greater than 0.7%.

In an embodiment, the percent silkinthe solution is greater than0.8%.

In anembodiment, the percent silkinthe solution is greater than 0,9%.

In an embodiment, the percent silk in the solution is greater than 1%.

In an embodiment, the percent silk in the solution is greater than 2%.

Inan embodiment, thepercent silkin the solutionis greater titan 3%.

In an embodiment, the percent silkin the solutionis greater than 4%.

In an embodiment, the percent silkin the solution is greater than 5%.

In an embodiment, the percent silk in the solution is greater than 6%.

In an embodiment, the percent silk in the solution is greater than 7%, Inan embodiment, thepercart silkinthesolutionis greater than 8%.

In an embodiment, the percent silkin the solution is greater than 9%.

In an ernbodiment, the percent silk in the solution is greater than In an embodiment, the percent silk in the solution is greater than 11%.

Inan embodiment, thepercent silkin the solutionis greater than 12%.

In an embodiment, the percent silk in the solution is greater than 13%.

In an embodiment, the percent silkin the solution is greater than 14%.

In an embodiment, the percent silk in the solutionis greater than 15%.

In an embodiment, the percent silk inthe solutionis greater than 16%.

In anembodiment, the percent silk inthe solutionis greater than 17%.

In an embodiment, the percent silk inthe solutionis greater titan 18%.

In an embodiment, the percent silk in the solution is greater than 19%.

In an embodiment, the percent silkinthe solutionis greater than 20%.

In anembodiment, the percent silkinthe solution is greater than 25%.

In an embodiment, the percent silk in die solution is between 0.1% and 30%.

In an embodiment, the percent silk in the solution is between 0.1% and 25%.

In an embodiment, the percent silk in the solutionis between 0.1% and 20%.

Inan embodiment, the percent silkin the solutionis between 0.1%and 15%.

In an embodiment, the percent silk in the solution is between 0.1% and 10%.

In an embodiment, thepercent silkin the solutionisbetween0.1%and 9%.

Inan embodiment, 1725 Date re?ue/Date received 2023-07-20 the percent siIk in the solution is between 0.1% and 8%.

In an embodiment, the percent silk in die solution is between 0.1% and 7%.

In an embodiment,Ihe percent silk in the solutionisbetween 0,1% and6.5%.

In an embodiment, the percent silk in the solution is between 0.1% and 6%.

In an embodiment, the percent silk in the solution is between 0.1% and 5.5%.

In an embodiment, the percent silk inthe solutionis between 0.1% and 5%.

In an embodiment, the percent silk inthe solutionis between 0.1% and 4.5%.

In an embodiment, thepercent silkinthe solutionis between 0.1%and 4%.

Inan embodiment, thepercent siIkin the solution is between 0.1% and 3.5%.

In an embodiment, thepercent silk in the solution is between 0,1% and 3%.

In an embodiment, the percent silk in the solutionisbetween 0.1% and 2.5%.

In an embodiment, the percent silkin the solutionis between 0.1% and 2.0%.

In an embodiment, the percent silk in the solution isbetween 0.1% and 2.4%.

In an embodiment, the percent silk inthe solutionis between 0.5% and 5%.

Inan embodiment, the percent silkinthe solutionis between0.5% and 4.5%.

In an embodiment, thepercent silkin the solutionisbetween0.5%and 4%.

Inan embodiment, thepercent silkinthe solutionis between0.5% and 3.5%.

In an embodiment, thepercent silk in the solution is between 0.5% and 3%.

In an embodiment, the percent silk in the solutionisbetween 0.5% and 2.5%.

In an embodiment, the percent silk inthe solution is between1and 4%.

In an embodiment, the percent silk in tire solutionis between 1 and 3.5%.

In an embodiment, the percent silk in the solution is between 1 and 3%.

In an embodiment, the percent silk in the solution is between 1 and 2.5%.

In an embodiment, the percent silkinthe solutionis between1and 2.4%.

In anembodiment,the percent silk inthe solutionisbetween 1 and 2%.

In anembodiment, thepercent silkinthe solutionis between 20% and 30%.

In an embodiment, the percent silk in the solution is between 0.1% and 6%.

In an embodiment, the percent silk in the solution is between 6% and 10%.

In an embodiment, the percent silk in the solution is between 6% and 8%.

In an embodiment, the percent silk in the solution is between 6% and 9A.

In an embodiment, the percent silkin the solution is between 10% and 20%.

In an embodiment, the percent silk in the solution is between 11% and 19%.

In an embodiment, die percent silk in the solutionisbetween 12% and 18%.

In an embodiment, the percent silk in the solution is between 13% and IM.

In an embodiment, the percent silk in the solution is between 1735 Date re?ue/Date received 2023-07-20 14% and 16%, In an embodiment, the percent silk in the solution is 2,4%, In an embodimort, thepercent silkin the solutionis hian embodiment, thepercent sericininthe solutionisnon-detectable to30%.

In an embedment, the percent sericin in the solution is non-detectable to 5%.

In an embodiment, the percent sericin in the solution is 1%.

In an embodiment, the percent sericin inthe solutionis 2%.

In an embodiment, the percent sericinin the solutionis 3%.

In an embodiment, the percent sericin in the solution is 4%.

In an embodiment, the percent sericin in the solution is 5%, In an embodiment, the percent sericin in the solutionis10%.

In anembodiment, the percent sericinin the solutionis 30%.

In an embodiment, the stability of the LiBr-sdlk fragment solution is 0 to 1year.

In an embodiment, the stability of the LiBr-silk fragment solutionis 0 to 2 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 0 to 3 years In an embodiment, the stability of the LiBr-silk fragment solution is 0 to 4 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 0 to 5 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 1 to 2 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 1 to 3 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 1 to 4 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 1 to 5 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 2 to 3 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 2 to 4 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 2 to 5 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 3 to 4 years.

In an embodiment, the stability of the LiBr-silk fragment solution is 3 to 5 years.

In an embodiment, the stability ofthe HBr-siIk fragment solutionis 4 to 5 years.

In an embodiment, the stalrility of a composition of the present disclosure is 10 days to 6 months.

In an embodiment, tire stability of a composition of the present disclosureis 6 months to 12months.

In an embodiment, the stability of a composition of the present disclosure is 12 months to 18 months.

In an embodiment, the stability of a composition of1he present disclosure is 18 months to 24 months.

In an embodiment, the stability of a composition of the present disclosure is 24 months to 30 months.

In an 174DEMANDE OU BREVET VOLUMINEUX LA PRESENTE PARTIE DE CETTE DEMANDE OU CE BREVET COMPREND PLUS D’UN TOME.

CECI EST LE TOME 1 DE 2 CONTENANT LES PAGES 1 A 174 NOTE : Pour les tomes additionels, veuillez contacter le Bureau canadien des brevets JUMBO APPLICATIONS/PATENTS THIS SECTION OF THE APPLICATION/PATENT CONTAINS MORE THAN ONE VOLUME THIS IS VOLUME 1 OF 2 CONTAINING PAGES 1 TO 174 NOTE: For additional volumes, please contact the Canadian Patent Office NOM DU FICHIER / FILE NAME : NOTE POUR LE TOME / VOLUME NOTE:

Claims (29)

1. CLAIMS We claim: 1. A method of coating a material with silk fibroin to provide a silk fibroin coated material, wherein the silk fibroin coated upon the silk fibroin coated material is heat resistant to a selected temperature, the method comprising: (a) preparing a silk fibroin solution comprising silk fibroin fragments having a weight average molecular weight selected from the group consisting of about 5 to about 10 kDa, about 6 kDa to about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, wherein the silk fibroin fragments have a polydispersity ranging from 1.5 to about 3; (b) coating one or more surfaces of the material with the silk fibroin solution; and (c) drying the one or more surfaces of the material that have been coated with the silk fibroin solution to provide the silk fibroin coated material, wherein drying the one or more surfaces of the material comprises heating the one or more surfaces of the material, wherein the silk fibroin fragments, prior to coating the material, do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in a solution for at least 10 days.
2. 2. The method of claim 1, wherein the silk fibroin fragments have a weight average molecular weight of about 6 kDa to about 16 kDa.
3. 3. The method of claim 1, wherein the silk fibroin fragments have a weight average molecular weight of about 39 kDa to about 80 kDa.
4. 4. The method of claim 1, wherein the silk fibroin fragments have a weight average molecular weight of about 5 kDa to about 10 kDa. 341 Date regue/Date received 2024-05-175.
5. The method of claim 1, wherein the silk fibroin fragments have a weight average molecular weight of about 17 kDa to about 38 kDa.
6. 6. The method of claim 1, wherein the silk fibroin fragments have a weight average molecular weight of about 60 kDa to about 100 kDa.
7. 7. The method of claim 1, wherein the silk fibroin fragments have a weight average molecular weight of about 80 kDa to about 144 kDa.
8. 8. The method of any one of claims 1-7, wherein the step of preparing the silk fibroin solution comprises adding a chemical fabric softener to the silk fibroin solution.
9. 9. The method of any one of claims 1-8, wherein the silk fibroin solution comprises a Bronsted acid.
10. 10. The method of any one of claims 1-9, wherein the silk fibroin solution comprises one or more of citric acid and acetic acid.
11. 11. The method of any one of claims 1-10, wherein the step of coating the one or more surfaces of the material comprises one or more of a roller application process, a saturation and removal process, and a topical application process. 342 Date regue/Date received 2024-05-1712.
12. The method of any one of claims 1-10, wherein the step of coating the one or more surfaces of the material comprises one or more of a bath coating process, a kiss rolling process, spray coating, and a two-sided rolling process.
13. 13. The method of any one of claims 1-12, further comprising the step of dyeing the one or more surfaces of the material prior to coating the one or more surfaces of the material with the silk fibroin solution.
14. 14. The method of any one of claims 1-12, further comprising the step of dyeing the one or more surfaces of the material after coating the one or more surfaces of the material with the silk fibroin solution.
15. 15. The method of any one of claims 1-14, wherein the material comprises one or more of a woven material, a non-woven material, a knit material, and a crochet material.
16. 16. The method of any one of claims 1-15, wherein the material comprises fabric, thread, yam, or a combination thereof.
17. 17. The method of any one of claims 1-16, wherein the material comprises one or more of polyester, polyamide, polyaramid, polytetrafluorethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethyleneglycol, ultrahigh molecular weight polyethylene, high-performance polyethylene, nylon, and spandex.
18. 18. The method of any one of claims 1-17, wherein the material comprises leather. 343 Date regue/Date received 2024-05-1719.
19. An article comprising a fabric or leather, wherein the fabric or leather is coated with a coating, wherein the coating comprises silk fibroin fragments having a weight average molecular weight selected from the group consisting of about 5 to about 10 kDa, about 6 kDato about 16 kDa, about 17 kDa to about 38 kDa, about 39 kDa to about 80 kDa, about 60 to about 100 kDa, and about 80 kDa to about 144 kDa, and wherein the silk fibroin fragments have a polydispersity ranging from 1.5 to about 3.
20. 20. The article of claim 19, wherein the fabric is selected from the group consisting of alpaca fleece, alpaca wool, lama fleece, lama wool, cotton, cashmere, sheep fleece, sheep wool, polyester, nylon, polyester-polyurethane copolymer, and combinations thereof.
21. 21. The article of claim 19, wherein the fabric comprises a polyester-polyurethane copolymer.
22. 22. The article of claim 21, wherein a portion of the silk fibroin is partially dissolved in a surface of the polyester-polyurethane copolymer.
23. 23. The article of claim 21 or 22, wherein the silk fibroin is crosslinked with the polyesterpolyurethane copolymer.
24. 24. The article of any one of claims 19 to 23, wherein the silk fibroin fragments have a weight average molecular weight of about 6 kDa to about 16 kDa.
25. 25. The article of any one of claims 19 to 23, wherein the silk fibroin fragments have a weight average molecular weight of about 39 kDa to about 80 kDa. 344 Date regue/Date received 2024-05-1726.
26. The article of any one of claims 19 to 23, wherein the silk fibroin fragments have a weight average molecular weight of about 5 kDa to about 10 kDa.
27. 27. The article of any one of claims 19 to 23, wherein the silk fibroin fragments have a weight average molecular weight of about 17 kDa to about 38 kDa.
28. 28. The article of any one of claims 19 to 23, wherein the silk fibroin fragments have a weight average molecular weight of about 60 kDa to about 100 kDa.
29. 29. The article of any one of claims 19 to 23, wherein the silk fibroin fragments have a weight average molecular weight of about 80 kDa to about 144 kDa. 345 Date regue/Date received 2024-05-17