CA3014584A1 - Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioreacteur a plateforme basculante - Google Patents

Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioreacteur a plateforme basculante Download PDF

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Publication number
CA3014584A1
CA3014584A1 CA3014584A CA3014584A CA3014584A1 CA 3014584 A1 CA3014584 A1 CA 3014584A1 CA 3014584 A CA3014584 A CA 3014584A CA 3014584 A CA3014584 A CA 3014584A CA 3014584 A1 CA3014584 A1 CA 3014584A1
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CA
Canada
Prior art keywords
cell
cells
cell aggregates
aggregates
passaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3014584A
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English (en)
Inventor
Brian Michael Davis
Kenneth Roger Conway
Xiaohua Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/075,211 external-priority patent/US10100277B2/en
Application filed by General Electric Co filed Critical General Electric Co
Publication of CA3014584A1 publication Critical patent/CA3014584A1/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne de nouvelles méthodes destinées à l'expansion et au repiquage d'agrégats cellulaires comprenant des cellules souches et/ou des cellules différenciées et comprenant l'utilisation de systèmes clos sur des bioréacteurs à plateforme basculante. Les méthodes de l'invention permettent une expansion du repiquage en série en système clos des cellules souches pluripotentes et/ou de leurs descendances avec des marqueurs pluripotents et un potentiel de différenciation.
CA3014584A 2016-03-21 2017-03-14 Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioreacteur a plateforme basculante Pending CA3014584A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/075,211 2016-03-21
US15/075,211 US10100277B2 (en) 2015-01-16 2016-03-21 Pluripotent stem cell expansion and passage using a stirred tank bioreactor
PCT/EP2017/055892 WO2017162467A1 (fr) 2016-03-21 2017-03-14 Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioréacteur à plateforme basculante

Publications (1)

Publication Number Publication Date
CA3014584A1 true CA3014584A1 (fr) 2017-09-28

Family

ID=58347333

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3014584A Pending CA3014584A1 (fr) 2016-03-21 2017-03-14 Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioreacteur a plateforme basculante

Country Status (5)

Country Link
EP (1) EP3433352A1 (fr)
JP (1) JP6943449B2 (fr)
CN (1) CN108779424A (fr)
CA (1) CA3014584A1 (fr)
WO (1) WO2017162467A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6815618B1 (ja) * 2019-03-15 2021-01-20 エイブル株式会社 培養システム及び培養方法
CN113785049B (zh) * 2019-06-10 2025-11-25 爱平世股份有限公司 细胞培养方法及单核细胞的回收方法
WO2021108243A1 (fr) * 2019-11-25 2021-06-03 Lonza Walkersville, Inc. Plate-forme intégrale pour la fabrication de cellules souches pluripotentes humaines
EP3835403A1 (fr) * 2019-12-11 2021-06-16 Sartorius Stedim Biotech GmbH Stratégie d'échange automatique de support pour cellules de suspension
KR20220128648A (ko) * 2020-01-14 2022-09-21 아지노모토 가부시키가이샤 세포의 배양 방법
CA3200169A1 (fr) * 2021-01-21 2022-07-28 Luis Haupt Procede de changement de milieu de culture d'une culture a l'aide de filtres a centrifuger
JP2022151855A (ja) * 2021-03-25 2022-10-07 株式会社カネカ 多能性幹細胞集団の製造方法
JPWO2022203051A1 (fr) * 2021-03-25 2022-09-29
JP2022151854A (ja) * 2021-03-25 2022-10-07 株式会社カネカ 多能性幹細胞集団の製造方法
TW202346574A (zh) * 2022-01-31 2023-12-01 日商希里歐斯股份有限公司 自然殺手細胞之製造方法
WO2025028596A1 (fr) * 2023-08-02 2025-02-06 株式会社ヘリオス Procédé de production de cellules tueuses naturelles
CN119432595A (zh) * 2023-08-07 2025-02-14 浙江霍德生物工程有限公司 利用生物反应器进行诱导多能干细胞的悬浮培养

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101517063A (zh) * 2006-07-28 2009-08-26 尤德斯·德克雷西 具有移动容器且以连续方式生产培养物的连续培养装置
WO2008136729A1 (fr) * 2007-05-04 2008-11-13 Sundstroem Erik Dispositif diviseur
WO2009006422A1 (fr) * 2007-06-29 2009-01-08 Stem Cell Products, Inc. Procédé automatisé et appareil permettant une culture de cellule souche embryonnaire
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CN102071137B (zh) * 2009-11-19 2014-10-22 上海坤巨科技发展有限公司 连续灌注生物反应器/罐袋系统制备干细胞装置和方法
CA2861270C (fr) * 2012-01-18 2021-08-03 Bayer Healthcare Llc Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes
MX2015004516A (es) * 2012-10-10 2015-10-14 Bayer Healthcare Llc Procedimientos y sistemas para optimizar el sistema de cultivo de celulas de perfusion.
US9738861B2 (en) * 2013-03-06 2017-08-22 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells

Also Published As

Publication number Publication date
JP6943449B2 (ja) 2021-09-29
EP3433352A1 (fr) 2019-01-30
JP2019509047A (ja) 2019-04-04
CN108779424A (zh) 2018-11-09
WO2017162467A1 (fr) 2017-09-28

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