JP6943449B2 - 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代 - Google Patents

撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代 Download PDF

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JP6943449B2
JP6943449B2 JP2018548844A JP2018548844A JP6943449B2 JP 6943449 B2 JP6943449 B2 JP 6943449B2 JP 2018548844 A JP2018548844 A JP 2018548844A JP 2018548844 A JP2018548844 A JP 2018548844A JP 6943449 B2 JP6943449 B2 JP 6943449B2
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cell
cells
cell aggregates
aggregates
closed system
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JP2019509047A (ja
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デイビス，ブライアン・マイケル
コンウェイ，ケネス・ロジャー
チャン，シャオフア
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Global Life Sciences Solutions USA LLC
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Amersham Biosciences Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity

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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2018548844A 2016-03-21 2017-03-14 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代 Active JP6943449B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/075,211 US10100277B2 (en) 2015-01-16 2016-03-21 Pluripotent stem cell expansion and passage using a stirred tank bioreactor
US15/075,211 2016-03-21
PCT/EP2017/055892 WO2017162467A1 (fr) 2016-03-21 2017-03-14 Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioréacteur à plateforme basculante

Publications (2)

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JP2019509047A JP2019509047A (ja) 2019-04-04
JP6943449B2 true JP6943449B2 (ja) 2021-09-29

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JP2018548844A Active JP6943449B2 (ja) 2016-03-21 2017-03-14 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代

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EP (1) EP3433352A1 (fr)
JP (1) JP6943449B2 (fr)
CN (1) CN108779424A (fr)
CA (1) CA3014584A1 (fr)
WO (1) WO2017162467A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6815618B1 (ja) * 2019-03-15 2021-01-20 エイブル株式会社 培養システム及び培養方法
WO2020250927A1 (fr) 2019-06-10 2020-12-17 アイ ピース， インコーポレイテッド Dispositif d'élimination d'érythrocytes, collecteur de cellules mononucléaires, dispositif de culture cellulaire, système de culture cellulaire, procédé de culture cellulaire et procédé de collecte de cellules mononucléaires
CA3156095A1 (fr) * 2019-11-25 2021-06-03 Lonza Walkersville, Inc. Plate-forme integrale pour la fabrication de cellules souches pluripotentes humaines
EP3835403A1 (fr) * 2019-12-11 2021-06-16 Sartorius Stedim Biotech GmbH Stratégie d'échange automatique de support pour cellules de suspension
CN114981411A (zh) * 2020-01-14 2022-08-30 味之素株式会社 细胞的培养方法
CA3200169A1 (fr) * 2021-01-21 2022-07-28 Luis Haupt Procede de changement de milieu de culture d'une culture a l'aide de filtres a centrifuger
JP2022151854A (ja) * 2021-03-25 2022-10-07 株式会社カネカ 多能性幹細胞集団の製造方法
WO2022203051A1 (fr) * 2021-03-25 2022-09-29 株式会社カネカ Procédé de production d'une population de cellules souches pluripotentes
JP2022151855A (ja) * 2021-03-25 2022-10-07 株式会社カネカ 多能性幹細胞集団の製造方法
US20250122474A1 (en) * 2022-01-31 2025-04-17 Healios K.K. Production method for natural killer cells
JPWO2025028596A1 (fr) * 2023-08-02 2025-02-06
CN119432595A (zh) * 2023-08-07 2025-02-14 浙江霍德生物工程有限公司 利用生物反应器进行诱导多能干细胞的悬浮培养

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101517063A (zh) * 2006-07-28 2009-08-26 尤德斯·德克雷西 具有移动容器且以连续方式生产培养物的连续培养装置
CA2686389C (fr) * 2007-05-04 2017-03-21 Erik Sundstroem Dispositif diviseur
DK2173863T3 (en) * 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CN102071137B (zh) * 2009-11-19 2014-10-22 上海坤巨科技发展有限公司 连续灌注生物反应器/罐袋系统制备干细胞装置和方法
EP2804943A1 (fr) * 2012-01-18 2014-11-26 Bayer HealthCare LLC Systèmes de bioréacteur de perfusion et procédés d'exploitation de ces systèmes
SG10201705806YA (en) * 2012-10-10 2017-08-30 Bayer Healthcare Llc Methods and systems for optimizing perfusion cell culture system
EP2966163B1 (fr) * 2013-03-06 2018-01-17 Kyoto University Système de culture pour des cellules souches pluripotentes et procédé pour la sous-culture de cellules souches pluripotentes

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Publication number Publication date
CA3014584A1 (fr) 2017-09-28
EP3433352A1 (fr) 2019-01-30
JP2019509047A (ja) 2019-04-04
WO2017162467A1 (fr) 2017-09-28
CN108779424A (zh) 2018-11-09

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