CA3041973A1 - Procede de mesure de cellules - Google Patents

Procede de mesure de cellules Download PDF

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Publication number
CA3041973A1
CA3041973A1 CA3041973A CA3041973A CA3041973A1 CA 3041973 A1 CA3041973 A1 CA 3041973A1 CA 3041973 A CA3041973 A CA 3041973A CA 3041973 A CA3041973 A CA 3041973A CA 3041973 A1 CA3041973 A1 CA 3041973A1
Authority
CA
Canada
Prior art keywords
image
cells
island
measurement method
light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3041973A
Other languages
English (en)
Inventor
Yuko NANJO
Hiroyuki Asano
Isao Miyagawa
Yoshio Takada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kurashiki Spinning Co Ltd
Original Assignee
Kurashiki Spinning Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kurashiki Spinning Co Ltd filed Critical Kurashiki Spinning Co Ltd
Publication of CA3041973A1 publication Critical patent/CA3041973A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/40—Extraction of image or video features
    • G06V10/60—Extraction of image or video features relating to illumination properties, e.g. using a reflectance or lighting model
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Multimedia (AREA)
  • Organic Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Optics & Photonics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Quality & Reliability (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Software Systems (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)

Abstract

Le proble`me de´crit par la pre´sente invention est de fournir un procédé de mesure de cellules de haute précision. La solution selon l'invention consiste en un procédé de mesure de cellules qui comprend : une étape de coloration de cellules cibles à l'aide d'un colorant; une étape d'acquisition d'image pour l'acquisition d'une image des cellules cibles; une étape de discrimination consistant à traiter l'image par le procédé de binarisation à étapes multiples afin de distinguer les cellules cibles des cellules contaminantes; une étape consistant à éliminer le bruit dû aux cellules contaminantes de l'image sur la base du résultat obtenu lors de l'étape de discrimination; et une étape visant à intégrer des valeurs d'indice de quantité de cellules dans l'image, dont les cellules contaminantes ont été éliminées, de façon à évaluer la quantité de cellules cibles.
CA3041973A 2016-11-01 2017-10-20 Procede de mesure de cellules Abandoned CA3041973A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016213995 2016-11-01
JP2016-213995 2016-11-01
PCT/JP2017/037954 WO2018084002A1 (fr) 2016-11-01 2017-10-20 Procédé de mesure de cellules

Publications (1)

Publication Number Publication Date
CA3041973A1 true CA3041973A1 (fr) 2018-05-11

Family

ID=62076550

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3041973A Abandoned CA3041973A1 (fr) 2016-11-01 2017-10-20 Procede de mesure de cellules

Country Status (6)

Country Link
US (1) US10920262B2 (fr)
JP (1) JP7088839B2 (fr)
CN (1) CN109890975A (fr)
CA (1) CA3041973A1 (fr)
TW (1) TWI733927B (fr)
WO (1) WO2018084002A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7678424B2 (ja) 2018-05-10 2025-05-16 学校法人順天堂 画像解析方法、装置、コンピュータプログラム、及び深層学習アルゴリズムの生成方法
WO2020009962A1 (fr) * 2018-07-02 2020-01-09 Ortho-Clinical Diagnostics, Inc. Analyse de lame sèche à l'aide d'une fenêtre de lecture réduite
JP7381003B2 (ja) * 2019-04-26 2023-11-15 学校法人順天堂 疾患解析を支援する方法、装置、及びコンピュータプログラム、並びにコンピュータアルゴリズムを訓練する方法、装置、及びプログラム
CN111321078B (zh) * 2020-03-05 2023-12-22 吉林大学 细胞培养箱及其控制方法和培养皿
CN114015741B (zh) * 2021-11-08 2024-01-30 中山大学 一种非侵入式的细胞活性分析方法
CN119413797B (zh) * 2024-09-09 2025-09-30 中国科学院苏州生物医学工程技术研究所 一种备液质量检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0728757B2 (ja) 1990-03-15 1995-04-05 新田ゼラチン株式会社 制癌剤感受性試験方法
US5356793A (en) * 1990-03-15 1994-10-18 Nitta Gelatin Inc. Method for testing the sensitivity of anticancer drug
WO1995018216A1 (fr) 1993-12-30 1995-07-06 Nitta Gelatin Inc. Procedes permettant d'enrober des cultures de cellules animales
JP3222785B2 (ja) * 1996-10-11 2001-10-29 新田ゼラチン株式会社 癌細胞の定量測定方法
JP2002312761A (ja) * 2001-04-12 2002-10-25 Matsushita Electric Ind Co Ltd 細胞画像の画像処理方法
JP3363445B1 (ja) * 2001-04-23 2003-01-08 新田ゼラチン株式会社 生検採取細胞の培養方法および動物細胞培養用キット
US7760925B2 (en) * 2004-03-16 2010-07-20 Kurashiki Boseki Kabushiki Kaisha Method for automatically detecting degenerated regions in stained thin section slides
WO2006001397A1 (fr) * 2004-06-25 2006-01-05 National Institute Of Advanced Industrial Science And Technology Système d’analyse de réseau de cellules
JP4866162B2 (ja) 2006-07-06 2012-02-01 倉敷紡績株式会社 抗がん作用の評価方法
JP2014063019A (ja) * 2012-09-21 2014-04-10 Keyence Corp 撮影解析装置、その制御方法及び撮影解析装置用のプログラム
JP2015092852A (ja) * 2013-11-12 2015-05-18 倉敷紡績株式会社 生体組織分散用組成物
WO2016136441A1 (fr) * 2015-02-23 2016-09-01 コニカミノルタ株式会社 Dispositif, procédé et programme de traitement d'images
JP6609954B2 (ja) * 2015-03-26 2019-11-27 東洋製罐グループホールディングス株式会社 Ｄｎａチップ画像のスポット有効性判定装置、ｄｎａチップ画像のスポット有効性判定方法、及びｄｎａチップ画像のスポット有効性判定プログラ
HK1248774A1 (zh) 2015-08-26 2018-10-19 仓敷纺绩株式会社 细胞测定方法

Also Published As

Publication number Publication date
JPWO2018084002A1 (ja) 2019-09-19
TW201818350A (zh) 2018-05-16
CN109890975A (zh) 2019-06-14
JP7088839B2 (ja) 2022-06-21
US10920262B2 (en) 2021-02-16
US20190256885A1 (en) 2019-08-22
WO2018084002A1 (fr) 2018-05-11
TWI733927B (zh) 2021-07-21

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20220420