CA3082861A1 - Procedes d'enrichissement et d'isolement de cellules - Google Patents

Procedes d'enrichissement et d'isolement de cellules Download PDF

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Publication number
CA3082861A1
CA3082861A1 CA3082861A CA3082861A CA3082861A1 CA 3082861 A1 CA3082861 A1 CA 3082861A1 CA 3082861 A CA3082861 A CA 3082861A CA 3082861 A CA3082861 A CA 3082861A CA 3082861 A1 CA3082861 A1 CA 3082861A1
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CA
Canada
Prior art keywords
magnetic field
holder
bag
cells
magnetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3082861A
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English (en)
Inventor
Weston Blaine Griffin
Xiaohua Zhang
Reginald Donovan Smith
Chengkun ZHANG
Kashan Ali Shaikh
Ye BAI
Susumu Mine
Minfeng Xu
Kenneth Roger Conway
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/829,615 external-priority patent/US10696961B2/en
Application filed by General Electric Co filed Critical General Electric Co
Publication of CA3082861A1 publication Critical patent/CA3082861A1/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3618—Magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/23—Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Mechanical Engineering (AREA)
  • Anesthesiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de biotraitement comprenant les étapes de combinaison d'une suspension comprenant une population de cellules avec des billes magnétiques pour former une population de cellules liées à des billes dans la suspension, isolant la population de cellules liées à des billes sur une colonne d'isolement magnétique, et collectant des cellules cibles à partir de la population de cellules. La collecte des cellules cibles consiste à déloger les cellules liées à des billes de la colonne d'isolement à l'aide d'une prise d'air. La présente invention concerne également un système comprenant un générateur de champ magnétique, un dispositif de maintien d'isolement de cellule magnétique, un kit fluidique jetable et des procédés associés pour leur utilisation.
CA3082861A 2017-12-01 2018-12-03 Procedes d'enrichissement et d'isolement de cellules Pending CA3082861A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US15/829,615 2017-12-01
US15/829,615 US10696961B2 (en) 2017-12-01 2017-12-01 Magnetic cell isolation techniques
US201862736151P 2018-09-25 2018-09-25
US201862736170P 2018-09-25 2018-09-25
US62/736,170 2018-09-25
US62/736,151 2018-09-25
PCT/EP2018/083382 WO2019106207A1 (fr) 2017-12-01 2018-12-03 Procédés d'enrichissement et d'isolement de cellules

Publications (1)

Publication Number Publication Date
CA3082861A1 true CA3082861A1 (fr) 2019-06-06

Family

ID=64606988

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3082861A Pending CA3082861A1 (fr) 2017-12-01 2018-12-03 Procedes d'enrichissement et d'isolement de cellules

Country Status (8)

Country Link
EP (1) EP3717622A1 (fr)
JP (2) JP7350741B2 (fr)
KR (2) KR20250076673A (fr)
CN (2) CN111757928A (fr)
AU (1) AU2018376657B2 (fr)
CA (1) CA3082861A1 (fr)
SG (1) SG11202004646TA (fr)
WO (1) WO2019106207A1 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7520322B2 (ja) 2018-02-09 2024-07-23 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 細胞療法のためのバイオプロセッシング方法
US12331276B2 (en) 2018-02-09 2025-06-17 Global Life Sciences Solutions USA Apparatus for fluid line management in a bioprocessing system
GB2629063B (en) * 2019-06-17 2025-02-12 Deka Products Lp System and method for cell and tissue preparation
GB201911687D0 (en) * 2019-08-15 2019-10-02 Fujifilm Diosynth Biotechnologies Uk Ltd Apparatus for purifying a liquid comprising a target substance
US11000780B1 (en) * 2019-12-06 2021-05-11 AnuCell Biosystems Limited Torus reactor for a combined cell isolator and bioreactor
US20210260600A1 (en) * 2020-02-01 2021-08-26 Sunil Mehta Automated device and method to purify biomaterials from a mixture by using magnetic particles and disposable product-contact materials
AU2021217829B2 (en) * 2020-02-06 2025-12-04 Cutiss Ag Cell isolation device and method
KR20230026340A (ko) * 2020-05-22 2023-02-24 에스큐지 바이오테크놀로지스 컴퍼니 세포를 자동으로 처리하기 위한 현장 의료 시스템
EP3936600A1 (fr) * 2020-07-06 2022-01-12 Sartorius Stedim Biotech GmbH Configuration automatique d'une centrifugeuse d'une installation de biotraitement
US20240392226A1 (en) * 2020-10-12 2024-11-28 Life Technologies As Magnetic particle processing systems for use with biological cells and related methods
JP2024503215A (ja) * 2020-12-15 2024-01-25 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 細胞の活性化、形質導入および増幅のための方法
CN116547371A (zh) 2020-12-15 2023-08-04 环球生命科技咨询美国有限责任公司 生物处理系统和方法
WO2022132724A1 (fr) 2020-12-15 2022-06-23 Global Life Sciences Solutions Usa Llc Appareil et procédé de surveillance en ligne d'un fluide de bioprocédé
WO2022132747A1 (fr) 2020-12-15 2022-06-23 Global Life Sciences Solutions Usa Llc Récipient de culture pour un système de biotraitement
EP4263788A1 (fr) 2020-12-15 2023-10-25 Global Life Sciences Solutions USA LLC Système et procédé de transfert de chaleur et de masse dans un système de biotraitement
JP2024503211A (ja) * 2020-12-15 2024-01-25 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 細胞洗浄、磁気単離および投与調整のための使い捨てキット
KR20230119645A (ko) 2020-12-15 2023-08-16 글로벌 라이프 사이언시즈 솔루션즈 유에스에이 엘엘씨 세포 농축, 단리 및 제형화를 위한 시스템 및 방법
EP4263789A1 (fr) 2020-12-15 2023-10-25 Global Life Sciences Solutions USA LLC Système et procédés de vérification de l'intégrité d'un système de biotraitement par mise sous pression
US20240102882A1 (en) 2020-12-15 2024-03-28 Global Life Sciences Solutions Usa Llc System and methods for verifying the integrity of a bioprocessing system using mass balancing techniques
WO2022132770A1 (fr) 2020-12-15 2022-06-23 Global Life Sciences Solutions Usa Llc Système, procédé et appareil pour mélanger un fluide dans un système de biotraitement
WO2022214558A1 (fr) * 2021-04-07 2022-10-13 Norgren LLC Systèmes de biotraitement
WO2023110682A2 (fr) * 2021-12-15 2023-06-22 Norgren LLC Système de biotraitement et collecteur de capteur associé
CN114563331B (zh) * 2022-03-22 2023-12-19 重庆市公共卫生医疗救治中心 基于微流控芯片的cd4+t淋巴细胞自动计数检测系统
CN114574323B (zh) * 2022-03-31 2023-04-07 江苏莱尔生物医药科技有限公司 一种细胞分离装置
WO2023215876A1 (fr) * 2022-05-06 2023-11-09 Tissue Genesis International Llc Procédé d'isolement d'îlots pancréatiques
US20250215372A1 (en) 2022-06-15 2025-07-03 Global Life Sciences Solutions Usa Llc Disposable kit and culture vessel for a bioprocessing system
WO2024047359A1 (fr) * 2022-08-31 2024-03-07 Cellular Origins Limited Système de remplissage/finition automatisé
US20250361483A1 (en) * 2022-10-24 2025-11-27 National Resilience, Inc. Systems, Methods, and Compositions for Selecting or Isolating Cells
WO2024215466A1 (fr) * 2023-04-12 2024-10-17 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Procédés et systèmes pour la perfusion des tissus
WO2025090532A1 (fr) * 2023-10-23 2025-05-01 Ctrl Therapeutics Usa, Inc. Plateformes, cartouches et procédés d'isolement et d'enrichissement de cellules cibles
WO2026057931A1 (fr) * 2024-09-10 2026-03-19 Sorbonne Universite Dispositif de detection d'au moins un composant present dans un fluide et procede correspondant

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7217565B2 (en) * 1999-02-12 2007-05-15 Stemcells California, Inc. Enriched central nervous system stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations
US6159378A (en) * 1999-02-23 2000-12-12 Battelle Memorial Institute Apparatus and method for handling magnetic particles in a fluid
US6764859B1 (en) * 1999-07-19 2004-07-20 Biomerieux, B.V. Device and method for mixing magnetic particles with a fluid
AU5967100A (en) * 1999-07-21 2001-02-13 Britta Christensen A novel method for the improved isolation of a target cell population
DE102004040785B4 (de) * 2004-08-23 2006-09-21 Kist-Europe Forschungsgesellschaft Mbh Mikrofluidisches System zur Isolierung biologischer Partikel unter Verwendung der immunomagnetischen Separation
CN101305087A (zh) * 2005-09-15 2008-11-12 阿尔特弥斯康复公司 用于磁富集细胞和其他颗粒的装置和方法
CN101172664A (zh) * 2006-11-01 2008-05-07 中国民航大学 磁性四氧化三铁纳米超细微粒的超声波辅助水热合成工艺
ES2660180T3 (es) * 2007-12-07 2018-03-21 Miltenyi Biotec Gmbh Sistemas y métodos para procesamiento de células
BR112012018166A2 (pt) * 2010-01-21 2015-09-15 Biocep Ltd separação magnética de células raras.
EP2412802A1 (fr) * 2010-07-29 2012-02-01 TXCell Cellules de type TR1 produisant de l'IL-13 et leurs utilisations
CN102174465A (zh) * 2011-01-12 2011-09-07 武汉格蓝丽富科技有限公司 一种从组织中分离富集靶细胞的方法
WO2013048546A1 (fr) * 2011-03-29 2013-04-04 Yongxin Zhang Système de bioréacteur multifonctionnel et procédés pour le triage et la culture cellulaires
EP2737317A2 (fr) * 2011-07-28 2014-06-04 The Trustees Of The University Of Pennsylvania Méthodes et réactifs pour le diagnostic d'états pathologiques et la caractérisation de cellules tumorales associées à des liquides séreux
CN102504991B (zh) * 2011-10-11 2013-12-04 中国人民解放军军事医学科学院卫生装备研究所 液样中细菌的非特异性快速富集纯化装置与方法
ES2602500T3 (es) * 2011-11-25 2017-02-21 Miltenyi Biotec Gmbh Método de separación celular
JP2013223820A (ja) * 2012-04-20 2013-10-31 Hitachi High-Technologies Corp 磁気分離装置およびこれを備えた自動分析装置、及び分離方法
US9797817B2 (en) * 2012-05-03 2017-10-24 The Regents Of The University Of Michigan Multi-mode separation for target detection
GB201208547D0 (en) * 2012-05-15 2012-06-27 Life Technologies As Sample holder
CN102690786B (zh) * 2012-06-05 2014-02-12 武汉格蓝丽富科技有限公司 一种细胞富集、分离、提取的方法和仪器及单细胞分析方法
CN104540594B (zh) * 2012-06-25 2019-07-02 通用医疗公司 使用高梯度磁场对粒子进行分类
FR3009082B1 (fr) * 2013-07-26 2016-10-21 Centre Nat Rech Scient Systeme microfluidique et procede pour isoler et quantifier au moins une sous-population de cellules a partir d'une population de cellules.
CN106062185A (zh) * 2014-04-24 2016-10-26 美天旎生物技术有限公司 用于自动生成遗传修饰的t细胞的方法
US20170268037A1 (en) * 2014-05-15 2017-09-21 Fluxion Biosciences, Inc. Methods and systems for cell separation using magnetic-and size-based separation
ES2981726T3 (es) * 2014-12-19 2024-10-10 Biosafe Sa Procesamiento secuencial de fluidos biológicos
EP3037170A1 (fr) * 2014-12-27 2016-06-29 Miltenyi Biotec GmbH Procédé de séparation de cellules multitris
EP3061529A1 (fr) * 2015-02-24 2016-08-31 AdnaGen GmbH Appareil et procédé pour l'analyse, l'isolement et/ou l'enrichissement de structures cibles dans un échantillon de fluide
CA2996529A1 (fr) * 2015-08-24 2017-03-02 Gpb Scientific, Llc Procedes et dispositifs de purification et de concentration de cellules multi-etapes
CN105606795B (zh) * 2015-12-31 2018-04-24 上海白泽医疗器械有限公司 一种细胞免疫磁珠分选系统
CN105651680A (zh) * 2016-02-04 2016-06-08 关节动力安达（天津）生物科技有限公司 鉴定不同免疫磁珠细胞分离器分选细胞效率的方法

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CN111757928A (zh) 2020-10-09
CN117343836A (zh) 2024-01-05
AU2018376657A1 (en) 2020-06-11
JP2023165752A (ja) 2023-11-17
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WO2019106207A9 (fr) 2019-08-01
KR20250076673A (ko) 2025-05-29
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