CN111757928A - 用于细胞富集和分离的方法 - Google Patents

用于细胞富集和分离的方法 Download PDF

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Publication number
CN111757928A
CN111757928A CN201880088322.2A CN201880088322A CN111757928A CN 111757928 A CN111757928 A CN 111757928A CN 201880088322 A CN201880088322 A CN 201880088322A CN 111757928 A CN111757928 A CN 111757928A
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magnetic field
bag
magnetic
holder
cell
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Chinese (zh)
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W.B.格里芬
张小华
R.D.史密斯
张呈昆
K.A.谢赫
白烨
三根进
徐民风
K.R.康维
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Globegroup Life Technology Consulting America Co ltd
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Globegroup Life Technology Consulting America Co ltd
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Priority claimed from US15/829,615 external-priority patent/US10696961B2/en
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Priority to CN202310912295.9A priority Critical patent/CN117343836A/zh
Publication of CN111757928A publication Critical patent/CN111757928A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3618—Magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/23—Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
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    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

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  • Bioinformatics & Cheminformatics (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880088322.2A 2017-12-01 2018-12-03 用于细胞富集和分离的方法 Pending CN111757928A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310912295.9A CN117343836A (zh) 2017-12-01 2018-12-03 用于细胞富集和分离的方法

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US15/829615 2017-12-01
US15/829,615 US10696961B2 (en) 2017-12-01 2017-12-01 Magnetic cell isolation techniques
US201862736151P 2018-09-25 2018-09-25
US201862736170P 2018-09-25 2018-09-25
US62/736170 2018-09-25
US62/736151 2018-09-25
PCT/EP2018/083382 WO2019106207A1 (fr) 2017-12-01 2018-12-03 Procédés d'enrichissement et d'isolement de cellules

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EP (1) EP3717622A1 (fr)
JP (2) JP7350741B2 (fr)
KR (2) KR20250076673A (fr)
CN (2) CN111757928A (fr)
AU (1) AU2018376657B2 (fr)
CA (1) CA3082861A1 (fr)
SG (1) SG11202004646TA (fr)
WO (1) WO2019106207A1 (fr)

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CN114574323A (zh) * 2022-03-31 2022-06-03 江苏莱尔生物医药科技有限公司 一种细胞分离装置

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US20210260600A1 (en) * 2020-02-01 2021-08-26 Sunil Mehta Automated device and method to purify biomaterials from a mixture by using magnetic particles and disposable product-contact materials
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KR20230026340A (ko) * 2020-05-22 2023-02-24 에스큐지 바이오테크놀로지스 컴퍼니 세포를 자동으로 처리하기 위한 현장 의료 시스템
EP3936600A1 (fr) * 2020-07-06 2022-01-12 Sartorius Stedim Biotech GmbH Configuration automatique d'une centrifugeuse d'une installation de biotraitement
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CN116547371A (zh) 2020-12-15 2023-08-04 环球生命科技咨询美国有限责任公司 生物处理系统和方法
WO2022132724A1 (fr) 2020-12-15 2022-06-23 Global Life Sciences Solutions Usa Llc Appareil et procédé de surveillance en ligne d'un fluide de bioprocédé
WO2022132747A1 (fr) 2020-12-15 2022-06-23 Global Life Sciences Solutions Usa Llc Récipient de culture pour un système de biotraitement
EP4263788A1 (fr) 2020-12-15 2023-10-25 Global Life Sciences Solutions USA LLC Système et procédé de transfert de chaleur et de masse dans un système de biotraitement
JP2024503211A (ja) * 2020-12-15 2024-01-25 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 細胞洗浄、磁気単離および投与調整のための使い捨てキット
KR20230119645A (ko) 2020-12-15 2023-08-16 글로벌 라이프 사이언시즈 솔루션즈 유에스에이 엘엘씨 세포 농축, 단리 및 제형화를 위한 시스템 및 방법
EP4263789A1 (fr) 2020-12-15 2023-10-25 Global Life Sciences Solutions USA LLC Système et procédés de vérification de l'intégrité d'un système de biotraitement par mise sous pression
US20240102882A1 (en) 2020-12-15 2024-03-28 Global Life Sciences Solutions Usa Llc System and methods for verifying the integrity of a bioprocessing system using mass balancing techniques
WO2022132770A1 (fr) 2020-12-15 2022-06-23 Global Life Sciences Solutions Usa Llc Système, procédé et appareil pour mélanger un fluide dans un système de biotraitement
WO2022214558A1 (fr) * 2021-04-07 2022-10-13 Norgren LLC Systèmes de biotraitement
WO2023110682A2 (fr) * 2021-12-15 2023-06-22 Norgren LLC Système de biotraitement et collecteur de capteur associé
WO2023215876A1 (fr) * 2022-05-06 2023-11-09 Tissue Genesis International Llc Procédé d'isolement d'îlots pancréatiques
US20250215372A1 (en) 2022-06-15 2025-07-03 Global Life Sciences Solutions Usa Llc Disposable kit and culture vessel for a bioprocessing system
WO2024047359A1 (fr) * 2022-08-31 2024-03-07 Cellular Origins Limited Système de remplissage/finition automatisé
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