CH283634A - Process for the preparation of a peptide-like derivative of the lysergic acid series. - Google Patents

Process for the preparation of a peptide-like derivative of the lysergic acid series.

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Publication number
CH283634A
CH283634A CH283634DA CH283634A CH 283634 A CH283634 A CH 283634A CH 283634D A CH283634D A CH 283634DA CH 283634 A CH283634 A CH 283634A
Authority
CH
Switzerland
Prior art keywords
methyl ester
histidine
preparation
lysergyl
lysergic acid
Prior art date
Application number
Other languages
German (de)
Inventor
Ag Sandoz
Original Assignee
Ag Sandoz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ag Sandoz filed Critical Ag Sandoz
Publication of CH283634A publication Critical patent/CH283634A/en

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Description

  

  Verfahren zur Darstellung eines     peptidartigen    Derivates der     Lysergsäure-Reihe.       Das vorliegende Patent betrifft ein Ver  fahren zur Darstellung von d -     Lysergyl-1-          histidin-rnethylester,welches    dadurch gekenn  zeichnet ist., dass     nian        d-Lysergsätire-azid    mit       1-Histidin-inethylester    kondensiert.

      <I>Beispiel:</I>  Eine Lösung von 1,0 g     d-Isolysergsäure-          azid    in 120     em3        Äther    mischt man mit einer       bösung    von 1,16 g     1-Ilistidin-iriethylester    (ent  sprechend 2 Äquivalenten, auf das     Azid    be  zogen) in einem Gemisch von 50     em3    Äther  und 10     em3        Isopropylalkoliol,    worin sich das       d-Isolysergsäure-azid    bis zu einem Gleichge  wicht in     d-Lysergsäure-azid    umlagert., und  lässt bei 20" C 12 Stunden stehen.  



  Nachdem man den     stickstoffwasserstoff-          sauren        Histidiri-methylester    durch Ausschüt  teln mit dreimal 20     em3    Wasser entfernt. hat,  dampft man die mit Natriumsulfat getrock  nete Reaktionslösung unter Vakuum ein. Das  Rohprodukt wird     ehromatographiseh    zerlegt.  Dazu wird der Rückstand in wenig Chloroform  gelöst und an eine Säule aus 300g     Ahimi-          niumoxyd    aufgezogen. Beim Entwickeln mit  dem gleichen Lösungsmittel werden wenig  dunkle Nebenprodukte ins Filtrat gewaschen.

    Beim Weiterentwickeln mit Chloroform, das       1%        Alkohol        enthält,        beobachtet        man        im        UV-          Licht    die Aufspaltung der breiten blauleuch  tenden Hauptzone in zwei getrennte Bänder.  Aus der besser haftenden Zone lassen sich  0,3 bis 0,5 g des     Lysergsäure-Deriv        ates        eluie-          ren.       Der     d-Ly        sergyl-l-histidin-metliylester    ist in  Alkohol, Aceton und Chloroform leicht, in  Benzol schwerlöslich.

   Er kristallisiert aus  keinem dieser     Lösungsmittel.     



  Beim Neutralisieren der alkoholischen Lö  sung der Base mit alkoholischer Salzsäure,  wobei zwei Äquivalente Sä     Lire    verbraucht wer  den, erhält man das     Dichlorhydrat,    das auf  Zusatz von Äther     auskristallisiert.    Das     d-          Ly        ser        gyl-l-Iristidin        -methylester-diclilorhy        drat,          C231-I2503N5    . 2     HCI,    schmilzt unscharf bei  1.90" C     (korr.)    unter Zersetzung. Das Salz  ist in Wasser ziemlich leicht. löslich.  



  [all' = +13  (e = 0,25 in     Methanol).  



  Process for the preparation of a peptide-like derivative of the lysergic acid series. The present patent relates to a process for the preparation of d-lysergyl-1-histidine-methyl ester, which is characterized in that nian d-lysergyl-azide condenses with 1-histidine-methyl ester.

      <I> Example: </I> A solution of 1.0 g of d-isolysergic acid azide in 120 em3 ether is mixed with a solution of 1.16 g of 1-ilistidine-iriethyl ester (corresponding to 2 equivalents, based on the azide pulled) in a mixture of 50 em3 ether and 10 em3 isopropyl alcohol, in which the d-isolysergic acid azide is rearranged to an equilibrium in d-lysergic acid azide., and left at 20 "C for 12 hours.



  After the histidiral methyl ester is removed by shaking with three times 20 cubic meters of water. the reaction solution getrock designated with sodium sulfate is evaporated under vacuum. The crude product is broken down by chromatography. To do this, the residue is dissolved in a little chloroform and drawn up on a column of 300 g ammonium oxide. When developing with the same solvent, a few dark by-products are washed into the filtrate.

    When developing further with chloroform, which contains 1% alcohol, the splitting of the broad blue luminous main zone into two separate bands can be observed in UV light. 0.3 to 0.5 g of the lysergic acid derivative can be eluted from the better adhering zone. The d-lysergyl-1-histidine methyl ester is easily soluble in alcohol, acetone and chloroform, and is sparingly soluble in benzene.

   It does not crystallize from any of these solvents.



  When the alcoholic solution of the base is neutralized with alcoholic hydrochloric acid, whereby two equivalents of acid are consumed, the dichlorohydrate is obtained, which crystallizes out on addition of ether. The d-ly ser gyl-l-iristidine methyl ester diclilorhydrate, C231-I2503N5. 2 HCI, melts indistinctly at 1.90 "C (corr.) With decomposition. The salt is quite easily soluble in water.



  [all '= +13 (e = 0.25 in methanol).

 

Claims (1)

PATENTANSPRUCH: Verfahren zur Darstellung von d-Ly sergy l- 1-histidin-methylester, dadurch gek'ennzeieh- net, dass man d-Lysergsäure-azid finit 1-Histi- din-methylester kondensiert. Die neue Verbindung, der d-Lysergyl-1- liistidin-niethylester, konnte nicht kristalli siert erhalten werden. PATENT CLAIM: Process for the preparation of d-Ly sergy l- 1-histidine methyl ester, characterized in that d-lysergic acid azide finite 1-histidine methyl ester is condensed. The new compound, the d-lysergyl-1-liistidine-diethyl ester, could not be obtained in crystallized form. Das d-Lysergyl-l- histidin-methy lester-dichlorhydrat besitzt die Bruttoformel C23H2503-NT5 . 2 HCl und schmilzt unscharf bei 190 C (korr., unter Zersetzung). Die spezifische Drehung des Dichlorhydrats beträgt [a] = +13" (in Me- D thanol). Der d-Ly sergyl-l-histidin-methy lester soll als Zwischenprodukt zur Herstellung thera peutisch wirksamer Verbindungen Verwen dung finden. The d-lysergyl-l-histidine-methyl ester dichlorohydrate has the gross formula C23H2503-NT5. 2 HCl and melts indistinctly at 190 C (corr., With decomposition). The specific rotation of the dichlorohydrate is [a] = +13 "(in methanol). The d-lysergyl-1-histidine-methyl ester is said to be used as an intermediate product for the production of therapeutically active compounds.
CH283634D 1948-09-17 1948-09-17 Process for the preparation of a peptide-like derivative of the lysergic acid series. CH283634A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH283634T 1948-09-17
CH269795T 1948-09-17

Publications (1)

Publication Number Publication Date
CH283634A true CH283634A (en) 1952-06-15

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CH283634D CH283634A (en) 1948-09-17 1948-09-17 Process for the preparation of a peptide-like derivative of the lysergic acid series.

Country Status (1)

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CH (1) CH283634A (en)

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