CH618985A5 - Process for the purification of protein conjugates. - Google Patents

Process for the purification of protein conjugates. Download PDF

Info

Publication number: CH618985A5
Authority: CH; Switzerland
Prior art keywords: protein; antibody; protein conjugates; conjugated; conjugation
Prior art date: 1974-07-02

Application number

CH857175A

Other languages

German (de)

English (en)

Inventor

Siegfried Dr Baudner

Heinz Haupt

Hans Merle

Original Assignee

Behringwerke Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1974-07-02

Filing date

1975-07-01

Publication date

1980-08-29

1975-07-01 Application filed by Behringwerke Ag filed Critical Behringwerke Ag

1980-08-29 Publication of CH618985A5 publication Critical patent/CH618985A5/de

Links

102000004169 proteins and genes Human genes 0.000 title claims description 48
108090000623 proteins and genes Proteins 0.000 title claims description 48
238000000034 method Methods 0.000 title claims description 32
230000008569 process Effects 0.000 title claims description 8
238000000746 purification Methods 0.000 title claims description 5
230000021615 conjugation Effects 0.000 claims description 18
238000001962 electrophoresis Methods 0.000 claims description 11
239000007850 fluorescent dye Substances 0.000 claims description 11
108010074605 gamma-Globulins Proteins 0.000 claims description 5
230000001900 immune effect Effects 0.000 claims description 4
150000002894 organic compounds Chemical class 0.000 claims description 3
239000006227 byproduct Substances 0.000 claims description 2
235000018102 proteins Nutrition 0.000 description 41
239000000975 dye Substances 0.000 description 19
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 13
238000002360 preparation method Methods 0.000 description 12
238000006243 chemical reaction Methods 0.000 description 10
230000002378 acidificating effect Effects 0.000 description 7
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 7
229920000915 polyvinyl chloride Polymers 0.000 description 7
239000004800 polyvinyl chloride Substances 0.000 description 7
239000000047 product Substances 0.000 description 6
238000000926 separation method Methods 0.000 description 5
108060003951 Immunoglobulin Proteins 0.000 description 4
239000012876 carrier material Substances 0.000 description 4
238000010166 immunofluorescence Methods 0.000 description 4
102000018358 immunoglobulin Human genes 0.000 description 4
102000004338 Transferrin Human genes 0.000 description 3
108090000901 Transferrin Proteins 0.000 description 3
238000004140 cleaning Methods 0.000 description 3
210000004201 immune sera Anatomy 0.000 description 3
229940042743 immune sera Drugs 0.000 description 3
239000000463 material Substances 0.000 description 3
230000005012 migration Effects 0.000 description 3
238000013508 migration Methods 0.000 description 3
239000012581 transferrin Substances 0.000 description 3
102000004506 Blood Proteins Human genes 0.000 description 2
108010017384 Blood Proteins Proteins 0.000 description 2
241000283707 Capra Species 0.000 description 2
229920006385 Geon Polymers 0.000 description 2
101000766306 Homo sapiens Serotransferrin Proteins 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
230000008901 benefit Effects 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
230000000694 effects Effects 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
238000010828 elution Methods 0.000 description 2
238000000605 extraction Methods 0.000 description 2
238000002523 gelfiltration Methods 0.000 description 2
229940072221 immunoglobulins Drugs 0.000 description 2
238000004255 ion exchange chromatography Methods 0.000 description 2
239000000843 powder Substances 0.000 description 2
239000011546 protein dye Substances 0.000 description 2
239000012460 protein solution Substances 0.000 description 2
XNSAINXGIQZQOO-SRVKXCTJSA-N protirelin Chemical compound NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H]1NC(=O)CC1)CC1=CN=CN1 XNSAINXGIQZQOO-SRVKXCTJSA-N 0.000 description 2
238000003908 quality control method Methods 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
102000002322 Egg Proteins Human genes 0.000 description 1
108010000912 Egg Proteins Proteins 0.000 description 1
241000283086 Equidae Species 0.000 description 1
241000282412 Homo Species 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
241001494479 Pecora Species 0.000 description 1
101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
239000002253 acid Substances 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
239000011324 bead Substances 0.000 description 1
230000027455 binding Effects 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
239000000969 carrier Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000009833 condensation Methods 0.000 description 1
239000012084 conversion product Substances 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
235000014103 egg white Nutrition 0.000 description 1
210000000969 egg white Anatomy 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000012757 fluorescence staining Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000008187 granular material Substances 0.000 description 1
230000008105 immune reaction Effects 0.000 description 1
238000011835 investigation Methods 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000000877 morphologic effect Effects 0.000 description 1
230000009871 nonspecific binding Effects 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
238000006116 polymerization reaction Methods 0.000 description 1
235000004252 protein component Nutrition 0.000 description 1
230000005855 radiation Effects 0.000 description 1
230000009467 reduction Effects 0.000 description 1
239000004576 sand Substances 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000000243 solution Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
235000013311 vegetables Nutrition 0.000 description 1
238000007693 zone electrophoresis Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/561—Immunoelectrophoresis

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
Analytical Chemistry (AREA)
General Health & Medical Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Biotechnology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Electrochemistry (AREA)
Biochemistry (AREA)
Cell Biology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Peptides Or Proteins (AREA)
Supplying Of Containers To The Packaging Station (AREA)
Making Paper Articles (AREA)
Folding Of Thin Sheet-Like Materials, Special Discharging Devices, And Others (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

CH857175A 1974-07-02 1975-07-01 Process for the purification of protein conjugates. CH618985A5 (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE2431719A DE2431719A1 (de)	1974-07-02	1974-07-02	Verfahren zur gewinnung von proteinkonjugaten

Publications (1)

Publication Number	Publication Date
CH618985A5 true CH618985A5 (en)	1980-08-29

Family

ID=5919498

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH857175A CH618985A5 (en)	1974-07-02	1975-07-01	Process for the purification of protein conjugates.

Country Status (15)

Country	Link
JP (1)	JPS5129222A (fr)
AT (1)	AT346365B (fr)
BE (1)	BE830923A (fr)
CA (1)	CA1064425A (fr)
CH (1)	CH618985A5 (fr)
DE (1)	DE2431719A1 (fr)
DK (1)	DK142147C (fr)
ES (1)	ES438882A1 (fr)
FR (1)	FR2277096A1 (fr)
GB (1)	GB1515392A (fr)
IE (1)	IE41485B1 (fr)
IL (1)	IL47594A (fr)
IT (1)	IT1039578B (fr)
LU (1)	LU72871A1 (fr)
NL (1)	NL7507620A (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4276140A (en) *	1980-01-10	1981-06-30	Ionics Inc.	Electrodialysis apparatus and process for fractionating protein mixtures
CH660368A5 (fr) *	1982-08-23	1987-04-15	Oreal	Derive proteinique comportant, en greffage, des restes absorbeurs d'ultra-violets, son procede de preparation et compositions le contenant.

1974
- 1974-07-02 DE DE2431719A patent/DE2431719A1/de not_active Ceased
1975
- 1975-06-26 NL NL7507620A patent/NL7507620A/xx not_active Application Discontinuation
- 1975-06-26 ES ES438882A patent/ES438882A1/es not_active Expired
- 1975-06-27 IL IL47594A patent/IL47594A/en unknown
- 1975-06-27 CA CA230,337A patent/CA1064425A/fr not_active Expired
- 1975-06-27 FR FR7520246A patent/FR2277096A1/fr active Granted
- 1975-06-30 LU LU72871A patent/LU72871A1/xx unknown
- 1975-06-30 IT IT24967/75A patent/IT1039578B/it active
- 1975-07-01 GB GB27687/75A patent/GB1515392A/en not_active Expired
- 1975-07-01 CH CH857175A patent/CH618985A5/de not_active IP Right Cessation
- 1975-07-01 DK DK298575A patent/DK142147C/da not_active IP Right Cessation
- 1975-07-01 AT AT504475A patent/AT346365B/de not_active IP Right Cessation
- 1975-07-01 IE IE1456/75A patent/IE41485B1/en unknown
- 1975-07-02 JP JP50081005A patent/JPS5129222A/ja active Pending
- 1975-07-02 BE BE157913A patent/BE830923A/fr not_active IP Right Cessation

Also Published As

Publication number	Publication date
DK298575A (da)	1976-01-03
BE830923A (fr)	1976-01-02
NL7507620A (nl)	1976-01-06
AU8263875A (en)	1977-01-06
GB1515392A (en)	1978-06-21
IE41485L (en)	1976-01-02
LU72871A1 (fr)	1977-03-07
FR2277096B1 (fr)	1979-03-23
ES438882A1 (es)	1977-01-16
ATA504475A (de)	1978-03-15
AT346365B (de)	1978-11-10
IL47594A0 (en)	1975-08-31
IL47594A (en)	1977-10-31
CA1064425A (fr)	1979-10-16
DK142147B (da)	1980-09-08
FR2277096A1 (fr)	1976-01-30
JPS5129222A (en)	1976-03-12
IT1039578B (it)	1979-12-10
DK142147C (da)	1981-02-09
DE2431719A1 (de)	1976-01-22
IE41485B1 (en)	1980-01-16

Legal Events

Date	Code	Title	Description
1988-03-31	PL	Patent ceased
2024-10-31	PL	Patent ceased

Publication	Publication Date	Title
DE3521679C2 (fr)	1989-08-24
DE2430357C2 (de)	1983-08-11	Verfahren zur qualitativen oder quantitativen Feststellung von Immunoglobulin der IgG-Klasse
DE3218348C2 (de)	1993-12-16	Verfahren zur Extraktion von Lactoferrin aus Lactoserum
EP1320747B1 (fr)	2006-03-01	Substance pour electrophorese analytique et experimentale
DE69128088T2 (de)	1998-03-19	Trennung der proteine und farbstoffe
DE3853224T2 (de)	1995-06-29	Produktion von Proteinen in aktiver Form.
DE19538625C2 (de)	1999-08-12	Verfahren zur Abtrennung von Albumin aus Serum durch Membranionenaustauschchromatographie
DE2640387C3 (de)	1981-01-22	Gewebespezifisches Protein und Verfahren zu dessen Herstellung
DE2557419A1 (de)	1976-06-24	Reagens zum nachweis von analyt- koerpern
DE19828466A1 (de)	1999-12-30	Entstörung von Immunoassays durch Substanzen, die aus den Framework-Regionen von Antikörpern abgeleitet sind
DE3887596T2 (de)	1994-05-19	Antikörper zum Propeptid des Prokollagen-III und seine Benutzung in einem Analyseverfahren.
DE2616984C3 (de)	1981-10-29	Verfahren zur Isolierung und Reinigung eines plazentenspezifischen Glycoproteins
CH618985A5 (en)	1980-08-29	Process for the purification of protein conjugates.
DE1767899A1 (de)	1971-09-30	Verfahren zur Isolierung von Antigenen und Antikoerpern
DE3809796A1 (de)	1989-10-05	Verfahren zum reinigen eines 168 kd-proteins von mycoplasma pneumoniae
CH639394A5 (de)	1983-11-15	Verfahren zur herstellung von immunglobulinpraeparationen mit verminderter komplementaktivitaet.
WO1999002566A2 (fr)	1999-01-21	Proteine normalisee
DE2448371A1 (de)	1975-05-07	Verfahren zur isolierung der transferrine aus biologischen stoffen
DE1767404C3 (de)	1975-02-27	Verfahren zur Gewinnung von Lysozym aus einem lysozymhaHigen Ausgangsmaterial
DE69316911T2 (de)	1998-05-28	Reine tsh zubereitung, verfahren zur herstellung und ihre anwendung für die herstellung von tsh spürelementen für tsh rezeptor nachweis und in tsh rezeptor testproben
DE102010054767B4 (de)	2013-02-21	Verfahren zur Trennung, Aufkonzentration und/oder Reinigung von (Blut)Plasmaprotein, Viren oder Virenbestandteilen
EP0766697A1 (fr)	1997-04-09	Procede permettant d'isoler des isolectines de gui
DE3028977A1 (de)	1981-07-02	Verfahren zur ausstellung von antikoerperserum mit erhoehter agglutinationsaktivitaet
DE2353035B2 (de)	1979-10-31	Verfahren zur Stabilisierung menschlicher oder tierischer Körperflüssigkeiten oder pflanzlicher Extrakte
DE19836213A1 (de)	2000-02-24	Verfahren zur Elution von Biomolekülen von Chromatographie-Materialien