CH663094A5 - Verfahren zur bestimmung des glukosegehaltes von nicht-enzymatisch glukosylierten proteinen und ein kit zur durchfuehrung des verfahrens. - Google Patents

Verfahren zur bestimmung des glukosegehaltes von nicht-enzymatisch glukosylierten proteinen und ein kit zur durchfuehrung des verfahrens. Download PDF

Info

Publication number: CH663094A5
Authority: CH; Switzerland
Prior art keywords: acid; solution; glucose; proteins; mixture
Prior art date: 1982-10-01

Application number

CH34884A

Other languages

German (de)

English (en)

Inventor

Bela Dr Szajani

Judit Dr Szelenyi

Janos Foeldi

Margit Dr Horanyi

Original Assignee

Reanal Finomvegyszergyar

Judit Dr Szelenyi

Janos Foeldi

Margit Dr Horanyi

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-10-01

Filing date

1984-01-26

Publication date

1987-11-13

1984-01-26 Application filed by Reanal Finomvegyszergyar, Judit Dr Szelenyi, Janos Foeldi, Margit Dr Horanyi filed Critical Reanal Finomvegyszergyar

1987-11-13 Publication of CH663094A5 publication Critical patent/CH663094A5/de

Links

238000000034 method Methods 0.000 title claims description 38
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims description 37
239000008103 glucose Substances 0.000 title claims description 37
102000004169 proteins and genes Human genes 0.000 title claims description 32
108090000623 proteins and genes Proteins 0.000 title claims description 32
MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 48
239000000203 mixture Substances 0.000 claims description 25
235000006408 oxalic acid Nutrition 0.000 claims description 16
HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 claims description 11
WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 claims description 10
239000002253 acid Substances 0.000 claims description 10
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 9
239000003599 detergent Substances 0.000 claims description 6
NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 claims description 5
238000005903 acid hydrolysis reaction Methods 0.000 claims description 5
RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 claims description 5
150000001299 aldehydes Chemical class 0.000 claims description 4
239000003054 catalyst Substances 0.000 claims description 3
150000002894 organic compounds Chemical class 0.000 claims description 3
DSLRVRBSNLHVBH-UHFFFAOYSA-N 2,5-furandimethanol Chemical compound OCC1=CC=C(CO)O1 DSLRVRBSNLHVBH-UHFFFAOYSA-N 0.000 claims description 2
239000011541 reaction mixture Substances 0.000 claims description 2
238000002798 spectrophotometry method Methods 0.000 claims description 2
230000003544 deproteinization Effects 0.000 claims 1
239000000243 solution Substances 0.000 description 36
108010054147 Hemoglobins Proteins 0.000 description 22
102000001554 Hemoglobins Human genes 0.000 description 22
230000031700 light absorption Effects 0.000 description 15
239000007788 liquid Substances 0.000 description 13
RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 11
210000004369 blood Anatomy 0.000 description 10
239000008280 blood Substances 0.000 description 10
239000003153 chemical reaction reagent Substances 0.000 description 10
239000006228 supernatant Substances 0.000 description 10
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
238000005259 measurement Methods 0.000 description 9
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 8
229940083575 sodium dodecyl sulfate Drugs 0.000 description 7
235000019333 sodium laurylsulphate Nutrition 0.000 description 7
239000000725 suspension Substances 0.000 description 7
238000010438 heat treatment Methods 0.000 description 6
108010088751 Albumins Proteins 0.000 description 5
102000009027 Albumins Human genes 0.000 description 5
230000002378 acidificating effect Effects 0.000 description 5
238000006243 chemical reaction Methods 0.000 description 5
238000000354 decomposition reaction Methods 0.000 description 5
239000012528 membrane Substances 0.000 description 5
210000002381 plasma Anatomy 0.000 description 5
230000008033 biological extinction Effects 0.000 description 4
239000012496 blank sample Substances 0.000 description 4
238000005119 centrifugation Methods 0.000 description 4
210000003743 erythrocyte Anatomy 0.000 description 4
238000011156 evaluation Methods 0.000 description 4
HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 4
239000002504 physiological saline solution Substances 0.000 description 4
239000002244 precipitate Substances 0.000 description 4
230000008569 process Effects 0.000 description 4
239000000047 product Substances 0.000 description 4
102000004506 Blood Proteins Human genes 0.000 description 3
108010017384 Blood Proteins Proteins 0.000 description 3
108060003951 Immunoglobulin Proteins 0.000 description 3
239000002202 Polyethylene glycol Substances 0.000 description 3
125000003277 amino group Chemical group 0.000 description 3
238000004587 chromatography analysis Methods 0.000 description 3
238000012937 correction Methods 0.000 description 3
230000002949 hemolytic effect Effects 0.000 description 3
102000018358 immunoglobulin Human genes 0.000 description 3
229920001223 polyethylene glycol Polymers 0.000 description 3
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
INGWEZCOABYORO-UHFFFAOYSA-N 2-(furan-2-yl)-7-methyl-1h-1,8-naphthyridin-4-one Chemical class N=1C2=NC(C)=CC=C2C(O)=CC=1C1=CC=CO1 INGWEZCOABYORO-UHFFFAOYSA-N 0.000 description 2
DNZPLHRZXUJATK-UHFFFAOYSA-N 2-sulfanylidene-5-[[5-[2-(trifluoromethyl)phenyl]furan-2-yl]methyl]-1,3-diazinane-4,6-dione Chemical compound FC(F)(F)C1=CC=CC=C1C(O1)=CC=C1CC1C(=O)NC(=S)NC1=O DNZPLHRZXUJATK-UHFFFAOYSA-N 0.000 description 2
239000004134 Dicalcium diphosphate Substances 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
229920005654 Sephadex Polymers 0.000 description 2
239000012507 Sephadex™ Substances 0.000 description 2
239000008351 acetate buffer Substances 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
230000023852 carbohydrate metabolic process Effects 0.000 description 2
235000021256 carbohydrate metabolism Nutrition 0.000 description 2
210000004027 cell Anatomy 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
238000001816 cooling Methods 0.000 description 2
206010012601 diabetes mellitus Diseases 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
150000002303 glucose derivatives Chemical class 0.000 description 2
229940072221 immunoglobulins Drugs 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
238000001727 in vivo Methods 0.000 description 2
238000005406 washing Methods 0.000 description 2
125000004042 4-aminobutyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])N([H])[H] 0.000 description 1
IYLLULUTZPKQBW-UHFFFAOYSA-N Acrinol Chemical compound CC(O)C(O)=O.C1=C(N)C=CC2=C(N)C3=CC(OCC)=CC=C3N=C21 IYLLULUTZPKQBW-UHFFFAOYSA-N 0.000 description 1
238000003691 Amadori rearrangement reaction Methods 0.000 description 1
SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
101710169606 Hemoglobin-2 Proteins 0.000 description 1
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
108010052285 Membrane Proteins Proteins 0.000 description 1
102000018697 Membrane Proteins Human genes 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
235000011054 acetic acid Nutrition 0.000 description 1
230000009471 action Effects 0.000 description 1
125000003172 aldehyde group Chemical group 0.000 description 1
239000003146 anticoagulant agent Substances 0.000 description 1
229940127219 anticoagulant drug Drugs 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
150000001491 aromatic compounds Chemical class 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
230000008859 change Effects 0.000 description 1
238000001311 chemical methods and process Methods 0.000 description 1
238000013375 chromatographic separation Methods 0.000 description 1
230000001684 chronic effect Effects 0.000 description 1
238000004737 colorimetric analysis Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
238000006482 condensation reaction Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
210000003617 erythrocyte membrane Anatomy 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
230000005714 functional activity Effects 0.000 description 1
108700004775 glucosylated hemoglobin A Proteins 0.000 description 1
108010004903 glycosylated serum albumin Proteins 0.000 description 1
229960002897 heparin Drugs 0.000 description 1
229920000669 heparin Polymers 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000003301 hydrolyzing effect Effects 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
239000008267 milk Substances 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
150000007522 mineralic acids Chemical class 0.000 description 1
229920002113 octoxynol Polymers 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000002265 prevention Effects 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000003756 stirring Methods 0.000 description 1
239000010414 supernatant solution Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Biomedical Technology (AREA)
Immunology (AREA)
Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Food Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Bioinformatics & Cheminformatics (AREA)
Bioinformatics & Computational Biology (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Investigating Or Analysing Biological Materials (AREA)

CH34884A 1982-10-01 1984-01-26 Verfahren zur bestimmung des glukosegehaltes von nicht-enzymatisch glukosylierten proteinen und ein kit zur durchfuehrung des verfahrens. CH663094A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
HU315582A HU186976B (en)	1982-10-01	1982-10-01	Process for determation of glucose containt of glucolized in non-ensimatic way proteins and reagent lutes for this process

Publications (1)

Publication Number	Publication Date
CH663094A5 true CH663094A5 (de)	1987-11-13

Family

ID=10962846

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH34884A CH663094A5 (de)	1982-10-01	1984-01-26	Verfahren zur bestimmung des glukosegehaltes von nicht-enzymatisch glukosylierten proteinen und ein kit zur durchfuehrung des verfahrens.

Country Status (5)

Country	Link
CH (1)	CH663094A5 (fr)
DE (1)	DE3414379A1 (fr)
FR (1)	FR2559267B1 (fr)
HU (1)	HU186976B (fr)
RO (1)	RO90093A (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5002893A (en) *	1985-09-19	1991-03-26	Isolab, Inc.	Single color reading method for determining fructosamine
DE4206932A1 (de) *	1992-03-05	1993-09-09	Boehringer Mannheim Gmbh	Immunologische methode zur bestimmung eines haemoglobinderivates

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1146769A (fr) *	1980-02-04	1983-05-24	Beat E. Glatthaar	Procede de depistage du diabete
US4349352A (en) *	1980-08-14	1982-09-14	Rockefeller University	Test for glucosylated hemoglobin and other glucosylated proteins
IT1167460B (it) *	1981-06-26	1987-05-13	Sclavo Inst Sieroterapeut	Dosaggio delle proteine glicosilate in fluidi biologici e mezzi adatti allo scopo
US4465774A (en) *	1981-07-16	1984-08-14	Sherwood Medical Company	Standard or control material for glysocylated or total hemoglobin determination

1982
- 1982-10-01 HU HU315582A patent/HU186976B/hu not_active IP Right Cessation
1984
- 1984-01-26 CH CH34884A patent/CH663094A5/de not_active IP Right Cessation
- 1984-02-06 FR FR8401745A patent/FR2559267B1/fr not_active Expired
- 1984-02-08 RO RO84113552A patent/RO90093A/fr unknown
- 1984-04-16 DE DE19843414379 patent/DE3414379A1/de not_active Withdrawn

Also Published As

Publication number	Publication date
RO90093A (fr)	1986-09-30
HU186976B (en)	1985-10-28
DE3414379A1 (de)	1985-10-17
FR2559267B1 (fr)	1988-03-04
HU32919A (fr)	1984-09-28
FR2559267A1 (fr)	1985-08-09

Legal Events

Date	Code	Title	Description
1992-09-30	PL	Patent ceased
2024-10-31	PL	Patent ceased

Publication	Publication Date	Title
Brennan	1989	Changes in solubility, non-enzymatic glycation, and fluorescence of collagen in tail tendons from diabetic rats
Gustavson	1954	Interaction of vegetable tannins with polyamides as proof of the dominant function of the peptide bond of collagen for its binding of tannins
DE3720736C2 (fr)	1990-04-19
EP0021152A1 (fr)	1981-01-07	Procédé pour la détermination immunologique d'une matière de membrane basale, fragments de membranes basales spécifiques pour ce procédé et procédé pour leur préparation, resp. leur obtention
EP0070992A1 (fr)	1983-02-09	Méthode de stabilisation de la peroxydase en milieu liquide et milieu stabilisé ainsi obtenu
DE19647927A1 (de)	1998-05-28	Verfahren zur Herstellung einer stabilen Troponin I Präparation und dessen Verwendung als Kalibrator in Immunoassays
CH663094A5 (de)	1987-11-13	Verfahren zur bestimmung des glukosegehaltes von nicht-enzymatisch glukosylierten proteinen und ein kit zur durchfuehrung des verfahrens.
Ward et al.	1961	The chemical and biochemical properties of fluorocitric acid
DE69430410T2 (de)	2002-12-05	Methode zum Nachweis von Fructosamin
DD270847A7 (de)	1989-08-16	Verfahren zur bestimmung des glukosegehaltes von nicht-enzymatisch glukosylierten proteinen und reagenssatz zur durchfuehrung des verfahrens
DE69610679T2 (de)	2001-05-03	Antigen für Enzymimmuntest und Verfahren zur Messung von erythrozytären Antikörpern
EP0033543B1 (fr)	1985-01-02	Procédé pour la détermination d'une sorte spéciale d'hémoglobine et utilisation dudit procédé pour la détection du diabète
EP0011107B1 (fr)	1983-06-22	Méthode pour la détermination d'un acide aminé ou aminosulfonique
EP0351790B1 (fr)	1994-06-01	Méthode pour la détermination de fructosamine
DE3223837A1 (de)	1983-01-13	Verfahren und ausruestung zur bestimmung von glykosilierten proteinen in biologischen fluiden oder fluessigkeiten
EP0349987B1 (fr)	1994-10-05	Solution standard pour fructosamine
EP1188055B1 (fr)	2004-04-14	Examen en laboratoire d'un echantillon de liquide organique ou de tissu effectue
Li	1944	Electrophoresis of Rat Sera1
Cross et al.	1973	Quantitative isolation and partial characterization of elastin in bovine muscle tissue
DE69637466T2 (de)	2009-03-26	Verfahren zur prüfung von antigenen, die mit durch mentalen stress bedingten erkrankungen assoziiert sind
DE69619915T2 (de)	2002-10-31	Immunologischer ELISA-Test für Inter-Alpha-Trypsin-Inhibitor
EP0809709B1 (fr)	2002-04-10	Necessaire d'amor age
Lenzi et al.	1985	An Optimized Method to Measure Glycosylproteins: HPLC Assay of 5-Hydroxymethyl-2-Furfuraldehyde (5-HMF)
EP0155676A2 (fr)	1985-09-25	Procédé d'obtention des domaines globulaires du collagène de la membrane basale et détermination immunologique de matériaux de la membrane basale et d'auto-anticorps
DE10125188A1 (de)	2002-11-28	Vorrichtung zum in vivo-Nachweis von oxidativem Stress durch Bestimmung von Aldehyden auf Thiobarbitursäure beschichteten Oberflächen