CL2016000572A1 - Proteína vainillil alcohol oxidasa recombinante (vaor) - Google Patents

Proteína vainillil alcohol oxidasa recombinante (vaor)

Info

Publication number
CL2016000572A1
CL2016000572A1 CL2016000572A CL2016000572A CL2016000572A1 CL 2016000572 A1 CL2016000572 A1 CL 2016000572A1 CL 2016000572 A CL2016000572 A CL 2016000572A CL 2016000572 A CL2016000572 A CL 2016000572A CL 2016000572 A1 CL2016000572 A1 CL 2016000572A1
Authority
CL
Chile
Prior art keywords
recombinant
vaor
alcohol oxidase
protein
vanillyl alcohol
Prior art date
Application number
CL2016000572A
Other languages
English (en)
Inventor
Fuentes Vergara Valery
Rodríguez Collazo Pedro
Álvarez Tejos Paola
Calderón Santa Cruz Rosario
Ehrenfeld Stolzenbach Nicole
Garretón Rodríguez Virginia
Original Assignee
Univ Santo Tomás
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Santo Tomás filed Critical Univ Santo Tomás
Priority to CL2016000572A priority Critical patent/CL2016000572A1/es
Publication of CL2016000572A1 publication Critical patent/CL2016000572A1/es
Priority to PCT/CL2017/050012 priority patent/WO2017152297A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención apunta a una proteína Vainillil alcohol oxidasa recombinante (VAOr) que resulta de la fusión de VAO y MBP (maltose binding protein, donde la VAO utilizada es el gen vaoA del hongo Penicillium simplicissimum. Esta proteína recombinante se expresa en E. coli, es funcional, con alto rendimiento, con alta actividad enzimática, estable en una conformación dimérica, donde la cola de histidina facilita la purificación de la enzima obtenida. Útil para producción natural de vainilla a gran escala.
CL2016000572A 2016-03-10 2016-03-10 Proteína vainillil alcohol oxidasa recombinante (vaor) CL2016000572A1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CL2016000572A CL2016000572A1 (es) 2016-03-10 2016-03-10 Proteína vainillil alcohol oxidasa recombinante (vaor)
PCT/CL2017/050012 WO2017152297A1 (es) 2016-03-10 2017-03-10 PROTEÍNA VAINILLIL ALCOHOL OXIDASA RECOMBINANTE (VAOr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2016000572A CL2016000572A1 (es) 2016-03-10 2016-03-10 Proteína vainillil alcohol oxidasa recombinante (vaor)

Publications (1)

Publication Number Publication Date
CL2016000572A1 true CL2016000572A1 (es) 2016-12-02

Family

ID=59788885

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2016000572A CL2016000572A1 (es) 2016-03-10 2016-03-10 Proteína vainillil alcohol oxidasa recombinante (vaor)

Country Status (2)

Country Link
CL (1) CL2016000572A1 (es)
WO (1) WO2017152297A1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE135403T1 (de) * 1987-03-10 1996-03-15 New England Biolabs Inc Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist
WO2015066722A1 (en) * 2013-11-04 2015-05-07 Bgn Tech Llc Methods of making vanillin via the microbial fermentation of ferulic acid from eugenol using a plant dehydrogenase.

Also Published As

Publication number Publication date
WO2017152297A1 (es) 2017-09-14

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