CL2017000528A1 - Descubrimiento y producción de proteínas biológicas condicionalmente activas en las mismas células hospederas eucariotas de producción - Google Patents

Descubrimiento y producción de proteínas biológicas condicionalmente activas en las mismas células hospederas eucariotas de producción

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Publication number
CL2017000528A1
CL2017000528A1 CL2017000528A CL2017000528A CL2017000528A1 CL 2017000528 A1 CL2017000528 A1 CL 2017000528A1 CL 2017000528 A CL2017000528 A CL 2017000528A CL 2017000528 A CL2017000528 A CL 2017000528A CL 2017000528 A1 CL2017000528 A1 CL 2017000528A1
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CL
Chile
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protein
production
wild
conditionally active
discovery
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CL2017000528A
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English (en)
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Jay M Short
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Bioatla Llc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
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    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K2317/00—Immunoglobulins specific features
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    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
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    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

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  • Gastroenterology & Hepatology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

<p>UN MÉTODO DE PREPARACIÓN DE UNA PROTEÍNA BIOLÓGICA CONDICIONALMENTE ACTIVA MEDIANTE LA SELECCIÓN DE UNA PROTEÍNA BIOLÓGICA SILVESTRE, EVOLUCIONANDO EL ADN QUE CODIFICA LA PROTEÍNA BIOLÓGICA SILVESTRE UTILIZANDO UNA O MÁS TÉCNICAS EVOLUTIVAS PARA CREAR ADN MUTANTES, EXPRESAR LOS ADN MUTANTES EN UNA CÉLULA HOSPEDERA EUCARIOTA DE PRODUCCIÓN PARA OBTENER UNA PROTEÍNA MUTANTE, SOMETER LA PROTEÍNA MUTANTE Y LA PROTEÍNA SILVESTRE A UN ENSAYO BAJO CONDICIONES FISIOLÓGICAS NORMALES Y A UN ENSAYO BAJO UNA CONDICIÓN ABERRANTE, SELECCIONAR UNA PROTEÍNA MUTANTE CONDICIONALMENTE ACTIVA, QUE EXHIBE AL MENOS UNO DE: (A) UNA DISMINUCIÓN DE LA ACTIVIDAD EN EL ENSAYO EN LA CONDICIÓN FISIOLÓGICA NORMA) EN COMPARACIÓN CON LA PROTEÍNA SILVESTRE, Y (B( UN AUMENTO DE LA ACTIVIDAD EN EL ENSAYO BAJO LA CONDICIÓN ABERRANTE EN COMPARACIÓN CON LA PROTEÍNA SILVESTRE; Y PRODUCIR LA PROTEÍNA BIOLÓGICA CONDICIONALMENTE ACTIVA EN LA MISMA CÉLULA HOSPEDERA EUCARIOTA DE PRODUCCIÓN USADA EN LA ETAPA DE EXPRESIÓN.</p>
CL2017000528A 2014-09-03 2017-03-03 Descubrimiento y producción de proteínas biológicas condicionalmente activas en las mismas células hospederas eucariotas de producción CL2017000528A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462045207P 2014-09-03 2014-09-03

Publications (1)

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CL2017000528A1 true CL2017000528A1 (es) 2017-11-03

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CL2017000528A CL2017000528A1 (es) 2014-09-03 2017-03-03 Descubrimiento y producción de proteínas biológicas condicionalmente activas en las mismas células hospederas eucariotas de producción

Country Status (11)

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US (2) US10513699B2 (es)
EP (1) EP3189152A4 (es)
JP (1) JP2017532019A (es)
KR (1) KR20170044115A (es)
CN (1) CN106795548A (es)
AU (2) AU2015311911B2 (es)
BR (1) BR112017004131A2 (es)
CA (1) CA2959786A1 (es)
CL (1) CL2017000528A1 (es)
MX (1) MX386703B (es)
WO (1) WO2016036916A1 (es)

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CA2959786A1 (en) 2016-03-10
BR112017004131A2 (pt) 2017-12-12
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