CL2017001929A1 - Método y producto - Google Patents

Método y producto

Info

Publication number
CL2017001929A1
CL2017001929A1 CL2017001929A CL2017001929A CL2017001929A1 CL 2017001929 A1 CL2017001929 A1 CL 2017001929A1 CL 2017001929 A CL2017001929 A CL 2017001929A CL 2017001929 A CL2017001929 A CL 2017001929A CL 2017001929 A1 CL2017001929 A1 CL 2017001929A1
Authority
CL
Chile
Prior art keywords
false
sequence
methods
analysis
double
Prior art date
Application number
CL2017001929A
Other languages
English (en)
Inventor
Knut Rudi
Robert C Wilson
Original Assignee
Norwegian Univ Of Life Sciences
Hedmark Univ Of Applied Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Norwegian Univ Of Life Sciences, Hedmark Univ Of Applied Sciences filed Critical Norwegian Univ Of Life Sciences
Publication of CL2017001929A1 publication Critical patent/CL2017001929A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

<p>LA PRESENTE INVENCIÓN SE REFIERE A UN MÉTODO QUE EVITA LA UNIÓN INDESEABLE DE DDNTP A POLINUCLEÓTIDOS DE DOBLE CADENA CUANDO ESTÁ EN PRESENCIA DE UNA POLIMERASA. TALES MÉTODOS PUEDEN USARSE PARA PREVENIR LA APARICIÓN DE FALSOS POSITIVOS EN MÉTODOS QUE EMPLEAN DDNTPS, POR EJEMPLO, EN MÉTODOS DE DETECCIÓN DE SECUENCIAS. LA PRESENTE INVENCIÓN TAMBIÉN PROPORCIONA UN MÉTODO PARA EVITAR UNA LECTURA FALSA DE TM O FALSOS EFECTOS DE FRET (TALES COMO SUPRESIÓN DE FALSOS POSITIVOS), POR EJEMPLO EN UN MÉTODO DE ANÁLISIS DE LA CURVA DE FUSIÓN. EN PARTICULAR, SE PROPORCIONA UN MÉTODO EN EL QUE SE DETECTA UNA SECUENCIA DE NUCLEÓTIDOS DIANA EN UN POLINUCLEÓTIDO DE PRUEBA UTILIZANDO UN MÉTODO EN EL QUE SE GENERA UNA MOLÉCULA DE DOBLE CADENA QUE PUEDE O NO COMPRENDER DOS ETIQUETAS DEPENDIENDO DE SI LA SECUENCIA DIANA ESTÁ PRESENTE EN LA QUE SE DETERMINA LA PRESENCIA DE LAS DOS ETIQUETAS, PREFERIBLEMENTE REALIZANDO UN ANÁLISIS DE LA CURVA DE FUSIÓN.</p>
CL2017001929A 2015-01-30 2017-07-27 Método y producto CL2017001929A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1501603.3A GB201501603D0 (en) 2015-01-30 2015-01-30 Method and product

Publications (1)

Publication Number Publication Date
CL2017001929A1 true CL2017001929A1 (es) 2018-06-15

Family

ID=52705543

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2017001929A CL2017001929A1 (es) 2015-01-30 2017-07-27 Método y producto

Country Status (12)

Country Link
US (1) US12595507B2 (es)
EP (1) EP3250711B8 (es)
JP (1) JP6837997B2 (es)
CN (1) CN107709573A (es)
AU (1) AU2016211132B2 (es)
CL (1) CL2017001929A1 (es)
DK (1) DK3250711T3 (es)
ES (1) ES2865436T3 (es)
GB (1) GB201501603D0 (es)
HK (1) HK1251024A1 (es)
PL (1) PL3250711T3 (es)
WO (1) WO2016120494A1 (es)

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WO2018185105A1 (en) * 2017-04-03 2018-10-11 Bio-Me As Microorganism detection methods
CN109735607A (zh) * 2017-10-31 2019-05-10 中国科学院上海巴斯德研究所 一种双脱氧核苷封闭的寡核苷酸的制备方法及其应用
GB201817889D0 (en) 2018-11-01 2018-12-19 Bio Me As Microorganism detection methods
JP7097849B2 (ja) * 2019-04-23 2022-07-08 ジェネシスヘルスケア株式会社 過敏性腸症候群のリスクを判定する方法
KR20240101595A (ko) * 2021-10-18 2024-07-02 트위스트 바이오사이언스 코포레이션 연속 서열분석에 관한 방법 및 조성물
CN114015766B (zh) * 2021-11-18 2024-04-05 银丰基因科技有限公司 一种心脑血管疾病精准用药的检测试剂盒
CN115478074A (zh) * 2022-11-01 2022-12-16 东南大学 一种基于催化发夹自组装反应的miRNA检测探针及其应用

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Also Published As

Publication number Publication date
EP3250711B8 (en) 2021-05-05
JP2018503404A (ja) 2018-02-08
DK3250711T3 (da) 2021-04-26
JP6837997B2 (ja) 2021-03-03
EP3250711A1 (en) 2017-12-06
PL3250711T3 (pl) 2021-11-15
CA2974458A1 (en) 2016-08-04
HK1251024A1 (zh) 2019-01-18
CN107709573A (zh) 2018-02-16
US12595507B2 (en) 2026-04-07
AU2016211132A1 (en) 2017-08-10
WO2016120494A1 (en) 2016-08-04
AU2016211132B2 (en) 2022-03-31
GB201501603D0 (en) 2015-03-18
ES2865436T3 (es) 2021-10-15
US20170369941A1 (en) 2017-12-28
EP3250711B1 (en) 2021-03-31

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