CL2017002092A1 - Modificación del sitio de unión del represor transcripcional del promotor nf-yc4 para un contenido de proteína aumentado y resistencia al estrés - Google Patents

Modificación del sitio de unión del represor transcripcional del promotor nf-yc4 para un contenido de proteína aumentado y resistencia al estrés

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Publication number
CL2017002092A1
CL2017002092A1 CL2017002092A CL2017002092A CL2017002092A1 CL 2017002092 A1 CL2017002092 A1 CL 2017002092A1 CL 2017002092 A CL2017002092 A CL 2017002092A CL 2017002092 A CL2017002092 A CL 2017002092A CL 2017002092 A1 CL2017002092 A1 CL 2017002092A1
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CL
Chile
Prior art keywords
binding site
protein content
transcriptional repressor
plant
promoter
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CL2017002092A
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English (en)
Inventor
Ling Li
Eve Syrkin Wurtele
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Univ Iowa State Res Found Inc
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Application filed by Univ Iowa State Res Found Inc filed Critical Univ Iowa State Res Found Inc
Publication of CL2017002092A1 publication Critical patent/CL2017002092A1/es

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    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract

<p>Método para aumentar el contenido de proteína en una célula eucariótica que comprende un gen NF—YC4 que comprende modificar el sitio de unión para el represor transcnpcional; el método para producir una planta con contenido de proteina aumentado que comprende cruzar y seleccionar el contenido de proteina aumentado; el método para aumentar Ia resistencia a un patógeno o una plaga en una planta que comprende el gen NF—YC4 que comprende modificar el sitio de unión para el represor transcripcional, solo o además en combinación con expresar QQS en Ia planta; el método para producir una planta con resistencia aumentada frente a un patôgeno o plaga que comprende cruzar y seleccionar para Ia resistencia aumentada al patógeno o a Ia plaga; una célula, colección de células, tejido, órgano, u organismo, donde el gen NF—YC4 comprende un promotor que comprende un sitio de unión para un represor transcripcional que ha sido modificado de modo que el represor transcripcional no puede prevenir Ia transcripción de NF—YC4; de híbridos de plantas; y sus semillas.</p>
CL2017002092A 2015-02-18 2017-08-16 Modificación del sitio de unión del represor transcripcional del promotor nf-yc4 para un contenido de proteína aumentado y resistencia al estrés CL2017002092A1 (es)

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US201562117924P 2015-02-18 2015-02-18
US201562244131P 2015-10-20 2015-10-20

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US (4) US10640781B2 (es)
EP (2) EP3258774B1 (es)
JP (3) JP6785235B2 (es)
KR (2) KR102630802B1 (es)
CN (2) CN107532148B (es)
AU (2) AU2016220088C1 (es)
BR (1) BR122023024788A2 (es)
CA (1) CA2977215A1 (es)
CL (1) CL2017002092A1 (es)
HK (1) HK1248274A1 (es)
IL (2) IL254038B2 (es)
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Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10640781B2 (en) 2015-02-18 2020-05-05 Iowa State University Research Foundation, Inc. Modification of transcriptional repressor binding site in NF-YC4 promoter for increased protein content and resistance to stress
JP7075170B2 (ja) * 2018-01-23 2022-05-25 インスティチュート フォー ベーシック サイエンス 延長された単一ガイドrna及びその用途
WO2019204253A1 (en) 2018-04-18 2019-10-24 Pioneer Hi-Bred International, Inc. Genes, constructs and maize event dp-202216-6
BR112020021364A2 (pt) 2018-04-18 2021-01-19 Pioneer Hi-Bred International, Inc. Aprimoramento de características agronômicas em milho por modificação de fatores de transcrição de mads box endógena
CN110894505A (zh) * 2018-08-23 2020-03-20 中国农业科学院作物科学研究所 一种培育抗大豆花叶病毒的大豆的方法
KR101994831B1 (ko) * 2018-10-02 2019-07-01 주식회사 바이오메딕 감귤 트리스테자 바이러스 저항성 유전자좌의 유전형 결정용 멀티플렉스 프라이머 세트 및 이의 용도
US20220119827A1 (en) * 2018-10-31 2022-04-21 Pioneer Hi-Bred International, Inc. Genome editing to increase seed protein content
US20230104317A1 (en) * 2020-01-31 2023-04-06 The Board Of Trustees Of The Leland Stanford Junior University Systems and Methods to Identify Genetic Silencers and Applications Thereof
CN112301038B (zh) * 2020-10-26 2023-06-13 吉林农业大学 人参wrky64-04基因及其应用
CN116888752A (zh) 2021-02-10 2023-10-13 株式会社Aesc日本 电池
CN113528518B (zh) * 2021-06-28 2022-07-15 西南大学 抑制核盘菌的miRNA及其应用
CA3255876A1 (en) 2022-05-27 2023-11-30 Genxtraits, Inc. NEW METHODS FOR IDENTIFYING AND USING UPFRONT OPEN READING FRAMEWORKS
CN116790634B (zh) * 2023-06-19 2024-04-16 昆明理工大学 一种锌指转录因子基因及其应用
CN118421693B (zh) * 2024-04-25 2024-12-10 广东省农业科学院设施农业研究所 CaCP450基因对黄瓜疫病的抗性及应用
CN118460595B (zh) * 2024-05-17 2026-02-13 中国农业大学 紫花苜蓿MsNF-YC4基因提高植物耐逆性的应用
CN119372214B (zh) * 2024-11-22 2025-09-16 南京林业大学 毛竹PeTEM1基因及其表达蛋白和应用
CN119639764A (zh) * 2024-12-13 2025-03-18 扬州大学 一种草莓抗逆及果品调控基因FaCOL1及其应用
CN120866352B (zh) * 2025-08-29 2026-04-24 湖北省农业科学院果树茶叶研究所 枳PtNF-YA3基因及其在植物抗旱遗传改良中的应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618988A (en) 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
IL101508A0 (en) 1991-04-08 1992-12-30 Rhone Poulenc Agrochimie Chimeric plant genes based on upstream regulatory elements of helianthinin
US8030546B2 (en) 1998-09-22 2011-10-04 Mendel Biotechnology, Inc. Biotic and abiotic stress tolerance in plants
US20050086718A1 (en) 1999-03-23 2005-04-21 Mendel Biotechnology, Inc. Plant transcriptional regulators of abiotic stress
WO2004087878A2 (en) 2003-03-28 2004-10-14 Monsanto Technology, Llc Novel plant promoters for use in early seed development
JP2005304409A (ja) 2004-04-23 2005-11-04 Yamasa Shoyu Co Ltd 表皮に傷を付けた丸大豆及びその利用
AR061685A1 (es) 2006-06-23 2008-09-17 Monsanto Technology Llc Plantas de cultivo transgenicas con mayor tolerancia al estres
CA2659252A1 (en) * 2006-08-07 2008-02-21 Mendel Biotechnology, Inc. Plants with enhanced size and growth rate
EP1917858A1 (en) * 2006-09-29 2008-05-07 Syngeta Participations AG A method for enhancing plant tolerance
JP5735775B2 (ja) * 2009-10-21 2015-06-17 花王株式会社 改変プロモーター
CN103403169A (zh) * 2011-02-24 2013-11-20 衣阿华州立大学研究基金公司 改变植物中生物化学成分的材料和方法
US10415053B2 (en) 2011-02-24 2019-09-17 Iowa State University Research Foundation, Inc. Materials and method for increasing a plant's resistance to stress
CN103509094B (zh) * 2012-06-25 2015-09-02 中国农业科学院作物科学研究所 植物耐逆性相关蛋白GmNF-YC9及其编码基因与应用
US20140186957A1 (en) * 2013-01-03 2014-07-03 Fred Hutchinson Cancer Research Center Engineered tal effector proteins with enhanced dna targeting capacity
KR101380049B1 (ko) * 2013-02-12 2014-04-01 경상대학교산학협력단 B―box 도메인 단백질들 및 이를 이용한 식물의 개화시기 조절방법
CN112301024A (zh) * 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
US10640781B2 (en) * 2015-02-18 2020-05-05 Iowa State University Research Foundation, Inc. Modification of transcriptional repressor binding site in NF-YC4 promoter for increased protein content and resistance to stress
CN112795572B (zh) * 2020-11-13 2022-12-27 中国农业大学 月季核因子RhNF-YC9及其在调控花瓣扩展中的应用
US20230151378A1 (en) * 2021-10-28 2023-05-18 Board Of Trustees Of Southern Illinois University Soybean Lines with High Seed Protein and Steady to High Oil Content

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