CL2017002314A1 - Métodos de preparación de polinucleótidos usando composiciones salinas de cationes multivalentes - Google Patents

Métodos de preparación de polinucleótidos usando composiciones salinas de cationes multivalentes

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Publication number
CL2017002314A1
CL2017002314A1 CL2017002314A CL2017002314A CL2017002314A1 CL 2017002314 A1 CL2017002314 A1 CL 2017002314A1 CL 2017002314 A CL2017002314 A CL 2017002314A CL 2017002314 A CL2017002314 A CL 2017002314A CL 2017002314 A1 CL2017002314 A1 CL 2017002314A1
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CL
Chile
Prior art keywords
polynucleotide
salt
composition
methods
multivalent cations
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CL2017002314A
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English (en)
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Premchandran H Ramiya
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Geron Corp
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Publication of CL2017002314A1 publication Critical patent/CL2017002314A1/es

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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
    • C12N2310/3145—Phosphoramidates with the nitrogen in 3' or 5'-position
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    • C12N2310/30—Chemical structure
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

<p>Los aspectos de la descripción incluyen métodos para la preparación de un polinucleótido. En algunas modalidades, el método incluye poner en contacto una primera composición de polinucleótidos que incluye: un polinucleótido que tiene una secuencia de 7 o más subunidades nucleósido donde al menos dos de las subunidades nucleósido están unidas por un enlace entre subunidades de tiofosforamidato N3'→P5'; y reactivos y productos sintéticos no objetivo; con una sal de cationes multivalente para precipitar una primera sal de polinucleótidos que incluye al menos un contraión de cationes multivalente; y separar la sal de polinucleótidos de la primera composición de polinucleótidos en contacto para producir una segunda composición de polinucleótidos que incluye la primera sal de polinucleótidos. En determinadas modalidades, el método incluye además poner en contacto la primera sal de polinucleótidos con un soporte de cromatografía de fase inversa; y eluir a partir del soporte de cromatografía una tercera composición de polinucleótidos que incluye una segunda sal de polinucleótidos. También se proporcionan composiciones que incluyen una sal del polinucleótido que incluye al menos un contraión de cationes multivalentes.</p>
CL2017002314A 2015-04-23 2017-09-13 Métodos de preparación de polinucleótidos usando composiciones salinas de cationes multivalentes CL2017002314A1 (es)

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US201562151891P 2015-04-23 2015-04-23

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CL2017002314A1 true CL2017002314A1 (es) 2018-05-04

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EP (1) EP3286203B1 (es)
JP (4) JP2018513127A (es)
KR (1) KR102401252B1 (es)
CN (2) CN107429247B (es)
AU (2) AU2016250576C1 (es)
BR (1) BR112017019627B1 (es)
CA (1) CA2978191C (es)
CL (1) CL2017002314A1 (es)
CO (1) CO2017009217A2 (es)
CY (1) CY1123197T1 (es)
DK (1) DK3286203T3 (es)
EA (1) EA035885B1 (es)
ES (1) ES2798270T3 (es)
HR (1) HRP20201218T1 (es)
HU (1) HUE051148T2 (es)
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MA (2) MA42157A (es)
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SG (2) SG11201707893RA (es)
SI (1) SI3286203T1 (es)
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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA42157A (fr) 2015-04-23 2018-02-28 Geron Corp Procédés de préparation de polynucléotides à l'aide de compositions de sel cationique multivalent
AU2016341309A1 (en) 2015-10-22 2018-06-07 Modernatx, Inc. Cancer vaccines
US11384352B2 (en) 2016-12-13 2022-07-12 Modernatx, Inc. RNA affinity purification
EP3554556B1 (en) 2016-12-19 2022-03-09 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
MA49914A (fr) * 2017-08-18 2021-04-21 Modernatx Inc Procédés analytiques par hplc
JP7441455B2 (ja) * 2017-09-22 2024-03-01 ザ リージェンツ オブ ザ ユニバーシティ オブ コロラド，ア ボディー コーポレイト 筋ジストロフィーの処置のためのチオモルホリノオリゴヌクレオチド
JP7727385B2 (ja) * 2017-12-18 2025-08-21 ヴェンタナ メディカル システムズ， インク． ペプチド核酸コンジュゲート

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4046720A (en) 1974-01-17 1977-09-06 California Institute Of Technology Crosslinked, porous, polyacrylate beads
US4413070A (en) 1981-03-30 1983-11-01 California Institute Of Technology Polyacrolein microspheres
US4678814A (en) 1981-03-30 1987-07-07 California Institute Of Technology Polyacrolein microspheres
US5185444A (en) 1985-03-15 1993-02-09 Anti-Gene Deveopment Group Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages
US4659774A (en) 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
DE69120821T2 (de) 1990-08-31 1997-01-23 The Regents Of The University Of Minnesota, Minneapolis, Minn. Polyethylenglykol-Derivate für Festphasenanwendungen
US5281701A (en) 1991-07-12 1994-01-25 Applied Biosystems, Inc. Process and compounds for RNA synthesis
US5476925A (en) 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
US5726297A (en) 1994-03-18 1998-03-10 Lynx Therapeutics, Inc. Oligodeoxyribonucleotide N3' P5' phosphoramidates
US5599922A (en) 1994-03-18 1997-02-04 Lynx Therapeutics, Inc. Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties
CZ274596A3 (en) 1994-03-18 1997-03-12 Lynx Therapeutics Oligonucleotide n3 - p5 phosphoramidates and process of their synthesis and hybridization
US5684143A (en) 1996-02-21 1997-11-04 Lynx Therapeutics, Inc. Oligo-2'-fluoronucleotide N3'->P5' phosphoramidates
US5859233A (en) 1996-02-21 1999-01-12 Lynx Therapeutics, Inc. Synthons for synthesis of oligonucleotide N3-P5 phosphoramidates
CA2274586A1 (en) 1996-12-20 1998-07-02 Geron Corporation Methods for detecting and inhibiting the rna component of telomerase
US5998604A (en) 1997-09-15 1999-12-07 The Perkin-Elmer Corporation Polynucleotide purification method
US6114519A (en) 1997-10-15 2000-09-05 Isis Pharmaceuticals, Inc. Synthesis of sulfurized oligonucleotides
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
CN1317291C (zh) 1999-09-10 2007-05-23 杰龙公司 寡核苷酸n3′→p5′硫代氨基磷酸酯,其合成及应用
JP2002060341A (ja) 2000-08-21 2002-02-26 Terumo Corp 止血剤
US7563618B2 (en) 2001-03-23 2009-07-21 Geron Corporation Oligonucleotide conjugates
CA2440322C (en) 2001-03-23 2014-09-09 Geron Corporation Oligonucleotide conjugates
PL1667522T3 (pl) * 2003-09-09 2018-06-29 Geron Corporation Zmodyfikowane oligonukleotydy do hamowania telomerazy
US20120329858A1 (en) 2010-08-04 2012-12-27 Geron Corporation Modified oligonucleotides for telomerase inhibition
TW200540180A (en) 2004-05-28 2005-12-16 Sankyo Co Telomerase inhibitor ena oligonucleotide
HRP20171705T1 (hr) 2004-07-02 2017-12-15 Geron Corporation Sinteza zaštićenih 3’-amino-nukleozidnih monomera
US20070212704A1 (en) * 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
JP5732196B2 (ja) * 2006-11-01 2015-06-10 バイオジェン アイデック エムエー インコーポレイティドＢｉｏｇｅｎ Ｉｄｅｃ Ｉｎｃ． 低ｐＨおよび二価カチオンを用いる生物高分子を単離する方法
BR112013003875B1 (pt) 2010-08-20 2021-10-05 Replicor Inc Composições farmacêuticas compreendendo complexo de quelato de oligonucleotídeo, formulação de oligonucleotídeo, e usos
US9106103B2 (en) 2011-09-23 2015-08-11 Eaton Corporation Unintteruptible power supply systems and methods employing on-demand energy storage
US9006630B2 (en) 2012-01-13 2015-04-14 Altasens, Inc. Quality of optically black reference pixels in CMOS iSoCs
AR091065A1 (es) 2012-05-18 2014-12-30 Replicor Inc Una formulacion farmaceutica que comprende un quelato de oligonucleotidos antiviral para el tratamiento de una infeccion antiviral
JP6450308B2 (ja) * 2012-06-14 2019-01-09 ライフ テクノロジーズ コーポレーション ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット
RS59363B1 (sr) * 2012-12-07 2019-11-29 Geron Corp Upotreba inhibitora telomeraze imetelstata za lečenje mijelofibroze
JOP20200257A1 (ar) * 2014-05-01 2017-06-16 Geron Corp تركيبات أوليجو نوكليوتيد وطرق لتحضيرها
MA42157A (fr) 2015-04-23 2018-02-28 Geron Corp Procédés de préparation de polynucléotides à l'aide de compositions de sel cationique multivalent

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SI3286203T1 (sl) 2020-10-30
BR112017019627B1 (pt) 2022-11-29
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CA2978191A1 (en) 2016-10-27
BR112017019627A2 (pt) 2018-05-15
JP2021152079A (ja) 2021-09-30
EP3286203B1 (en) 2020-05-06
WO2016172346A1 (en) 2016-10-27
EP3286203A1 (en) 2018-02-28
PH12017501927A1 (en) 2018-03-05
CN107429247B (zh) 2021-08-13
AU2016250576C1 (en) 2021-05-06
US20200339975A1 (en) 2020-10-29
MD3286203T2 (ro) 2020-08-31
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LT3286203T (lt) 2020-07-27
PE20171785A1 (es) 2017-12-27
JP2018513127A (ja) 2018-05-24
SMT202000389T1 (it) 2020-09-10
DK3286203T3 (da) 2020-06-02
CO2017009217A2 (es) 2018-02-20
CY1123197T1 (el) 2021-10-29
US20160312227A1 (en) 2016-10-27
MA42157A (fr) 2018-02-28
PH12017501927B1 (en) 2022-09-02
TW201706284A (zh) 2017-02-16
US11441144B2 (en) 2022-09-13
JP2025061848A (ja) 2025-04-11
PT3286203T (pt) 2020-07-08
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CN113564168B (zh) 2024-10-25
ME03811B (me) 2021-04-20
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KR102401252B1 (ko) 2022-05-24
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CA2978191C (en) 2022-10-04
JP2022168017A (ja) 2022-11-04
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