CN101167883A - A traditional Chinese medicine compound preparation for resisting gastric cancer metastasis and preparation method thereof - Google Patents

Abstract

The invention provides a Chinese medicine compound preparation for resisting gastric cancer metastasis and a preparation method thereof. The Chinese medicine compound preparation is made of the following Chinese medicine raw materials in parts by weight: 40-70 parts of Astragalus membranaceus, 30-60 parts of psoralen, Ligustrum lucidum 20-40 servings, 20-40 servings of wolfberry, 10-30 servings of Scrophulariaceae. The traditional Chinese medicine compound preparation has a good effect on inhibiting gastric cancer metastasis. The preparation method is as follows: mixing the above-mentioned traditional Chinese medicine raw materials by weight, adding 8-12 times the amount of water to soak for 1-2 hours, ultrasonically extracting for 10-30 minutes, filtering, and collecting the filtrate; adding 6-10 times the amount of water to the filter residue, ultrasonically extracting 10 After ~20 minutes, filter and collect the filtrate; add 4 to 8 times the amount of water to the filter residue, ultrasonically extract for 5 to 15 minutes, filter, and collect the filtrate; combine the filtrates three times, and use rotary evaporation to remove the solvent to make a dry extract. Baked at 80°C until completely dry, then pulverized into fine powder; adding the fine powder to various conventional auxiliary materials required for preparing different dosage forms to prepare the required dosage form of traditional Chinese medicine preparations.

Description

A traditional Chinese medicine compound preparation for resisting gastric cancer metastasis and preparation method thereof

technical field

The invention belongs to the field of traditional Chinese medicines, in particular to a compound traditional Chinese medicine preparation for resisting gastric cancer metastasis and a preparation method thereof.

Background technique

Malignant tumors are common and frequently-occurring diseases that seriously endanger people's health and life. Among them, gastric cancer is one of the more common tumors in humans, with a high incidence rate, ranking first in digestive tract tumors, and its death rate is the first among various cancers in my country. About 150,000 people die of gastric cancer in China every year, and the death rate of gastric cancer in my country is also relatively high in the world.

Metastasis of gastric cancer cells, like other tumor metastasis, is a complicated process. Tumor cells break away from the primary lesion, pass through the basement membrane and matrix into the circulatory system, then selectively adhere to vascular endothelial cells, and then pass through the vascular basement membrane. And destroy the extracellular matrix, and then enter the heterotopic organ, continue cell growth, division and angiogenesis, etc., forming metastatic lesions. There are many therapeutic targets in the whole process of metastasis, but the complete mechanism of anti-tumor metastasis is not completely clear, and further exploration and research are needed. The anti-tumor treatment of traditional Chinese medicine focuses on the whole, which is to comprehensively improve the body's immunity, improve blood hypercoagulability and microcirculation disorders, reduce the chance of thrombus, and reduce tissue edema. Many studies have also proved that traditional Chinese medicine and its active ingredients have good anti-tumor and anti-metastasis effects, and are one of the important adjuvant treatments for traditional anti-tumor and anti-metastasis treatments.

Chinese invention patent CN1457837A discloses a traditional Chinese medicine preparation that has an inhibitory effect on gastric cancer, but its shortcoming is that the metastasis effect on gastric cancer is not mentioned. Chinese invention patent CN1394646A discloses an anti-cancer traditional Chinese medicine powder and its preparation method. Its effect is to improve the immunity of the body and suppress and dissolve cancer cells. Its shortcoming is that whether it can resist cancer metastasis is not mentioned.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies of the prior art, and to provide a Chinese medicine compound preparation for resisting gastric cancer metastasis. It has a good effect on inhibiting the metastasis of gastric cancer.

Another object of the present invention is to provide a preparation method of a traditional Chinese medicine compound preparation for resisting gastric cancer metastasis.

The object of the present invention is achieved through the following technical solutions: a traditional Chinese medicine compound preparation for resisting gastric cancer metastasis, made of the following Chinese medicine raw materials in parts by weight: 40-70 parts of astragalus, 30-60 parts of psoralen, 20 parts of Ligustrum lucidum ～40 parts, 20～40 parts of Chinese wolfberry, 10～30 parts of Scrophulariaceae.

The traditional Chinese medicine compound preparation for resisting gastric cancer metastasis is preferably made of the following Chinese medicine raw materials in parts by weight: 55 parts of astragalus, 50 parts of psoralen, 30 parts of Ligustrum lucidum, 30 parts of wolfberry, and 20 parts of scrophulariaceae.

The dosage form of the traditional Chinese medicine compound preparation for anti-gastric cancer metastasis is a conventional oral preparation prepared by conventional methods of traditional Chinese medicine preparations, such as tablets, capsules, oral liquids, granules and the like.

The preparation method of the Chinese medicine compound preparation for anti-gastric cancer metastasis comprises the following steps:

(1) Mix astragalus, psoralen, wolfberry, scrophulariaceae and privet privet fruit according to the weight ratio, add 8 to 12 times the amount of water to soak for 1 to 2 hours, ultrasonically extract for 10 to 30 minutes, filter, and collect the filtrate Add 6 to 10 times the amount of water to the filter residue, ultrasonically extract for 10 to 20 minutes, filter, and collect the filtrate; add 4 to 8 times the amount of water to the filter residue, ultrasonically extract for 5 to 15 minutes, filter, and collect the filtrate; Make use of the solvent to make a dry extract, bake it in an oven at 70-80°C until it is completely dry, and then pulverize it into a fine powder.

(2) Add the fine powder to various conventional auxiliary materials required for preparing different dosage forms, and prepare the required dosage forms of Chinese medicine preparations according to conventional methods of Chinese medicine preparations.

In the step (1), it is preferred to mix astragalus, psoraleae, wolfberry, scrophulariaceae and Ligustrum lucidum according to the weight ratio, add 10 times the amount of water to soak for 1.5 hours, ultrasonically extract for 20 minutes, filter, and collect the filtrate; Add 8 times the amount of water to the filter residue, ultrasonically extract for 15 minutes, filter, and collect the filtrate; add 6 times the amount of water to the filter residue, ultrasonically extract for 10 minutes, filter, and collect the filtrate; combine the three filtrates, freeze-dry and crush into fine powder.

The traditional Chinese medicine compound preparation for resisting gastric cancer metastasis of the present invention selects astragalus, psoraleae, wolfberry, scrophulariaceae and Ligustrum lucidum to prepare active components. Among them, astragalus, psoraleae, privet fruit, and wolfberry have the effects of invigorating qi and liver and kidney, and crocodile has the effects of promoting blood circulation, removing blood stasis, cooling blood, detoxifying, dissipating blood stasis, and relieving pain. Modern studies have shown that the above medicinal flavors all have anti-tumor effects. The above-mentioned five herbs share the functions of invigorating qi, promoting blood circulation and removing blood stasis, and have the effect of resisting gastric cancer metastasis.

Compared with the prior art, the present invention has the following outstanding advantages:

The Chinese medicine compound preparation of the present invention is under the guidance of the theory of traditional Chinese medicine, aiming at gastric cancer patients with "Qi deficiency and blood stasis" syndrome, and taking "tonifying qi, activating blood and removing blood stasis" as the treatment principle, the compound preparation has a good effect on inhibiting the metastasis of gastric cancer. effect.

Detailed ways

The present invention will be further described in detail below in conjunction with the examples, but the embodiments of the invention are not limited thereto.

Example 1

(1) Weigh each raw material according to the following weight ratio (take ten times the ratio of raw materials as an example)

550 parts of astragalus, 500 parts of psoralen, 300 parts of Ligustrum lucidum, 300 parts of wolfberry, 200 parts of scrophulariaceae, set aside.

(2) Astragalus, psoraleae, wolfberry, scrophulariaceae and Ligustrum lucidum in the above weight ratio are soaked in 10 times the amount of water for 1.5 hours, ultrasonically extracted for 20 minutes, filtered, and the filtrate is collected; the filter residue is added with 8 times the amount of water, and ultrasonically extracted After 15 minutes, filter and collect the filtrate; add 6 times the amount of water to the filter residue, and ultrasonically extract for 10 minutes. The three filtrates were combined, and the solvent was removed by rotary evaporation to make a dry extract, which was baked in an oven at 75°C until completely dry and then pulverized into fine powder (passed through a 80-mesh sieve).

(3) Get 75 parts of above-mentioned subdivisions, add 5 parts of starch, 4 parts of 8% starch slurry, 12.5 parts of dry starch, 0.4 part of magnesium stearate, and the above-mentioned fractions are all parts by weight. The drug is subdivided and mixed with starch, and starch slurry is added to make a soft material, granulated with a 14-mesh sieve, dried at 70°C, granulated with a 12-mesh sieve, added with dry starch and magnesium stearate, mixed evenly, and compressed into tablets. Get the tablet.

Example 2

(1) Weigh each raw material according to the following weight ratio (take ten times the ratio of raw materials as an example)

400 parts of astragalus, 300 parts of psoralen, 200 parts of Ligustrum lucidum, 200 parts of wolfberry, and 100 parts of scrophulariaceae for later use.

(2) Astragalus, psoraleae, wolfberry, scrophulariae and Ligustrum lucidum in the above weight ratio are soaked in 8 times the amount of water for 1 hour, ultrasonically extracted for 10 minutes, filtered, and the filtrate is collected; the filter residue is added with 6 times the amount of water, and ultrasonically extracted After 10 minutes, filter and collect the filtrate; add 4 times the amount of water to the filter residue, and ultrasonically extract for 10 minutes. The three filtrates were combined, and the solvent was removed by rotary evaporation to make a dry extract, which was baked in an oven at 70°C until completely dry and then pulverized into fine powder (passed through a 80-mesh sieve).

(3) Take 75 parts of the above-mentioned subdivision, add 5 parts of starch, 8 parts of 10% starch slurry, and 10 parts of dry starch, and the above-mentioned fractions are all parts by weight. The drug is subdivided and mixed with starch, and starch slurry is added to make a soft material, granulated with a 14-mesh sieve, dried at 80°C, and granulated with a 12-mesh sieve to make granules, mixed evenly, and packed into hard gelatin capsules to obtain capsules .

Example 3

(1) Weigh each raw material according to the following weight ratio (take ten times the ratio of raw materials as an example)

700 parts of astragalus, 600 parts of psoralen, 400 parts of Ligustrum lucidum, 400 parts of wolfberry, and 300 parts of scrophulariaceae for later use.

(2) Astragalus, psoraleae, wolfberry, scrophulariaceae and Ligustrum lucidum in the above weight ratio were soaked in 12 times the amount of water for 2 hours, ultrasonically extracted for 30 minutes, filtered, and the filtrate was collected; the filter residue was added with 10 times the amount of water, and ultrasonically extracted After 20 minutes, filter and collect the filtrate; add 8 times the amount of water to the filter residue, and ultrasonically extract for 15 minutes. The three filtrates were combined, and the solvent was removed by rotary evaporation to make a dry extract, which was baked in an oven at 80°C until completely dry and then pulverized into fine powder (passed through a 80-mesh sieve).

(3) Take 67 parts of the above-mentioned subdivisions, add 12 parts of starch, 8 parts of 10% starch slurry, and 13 parts of dry starch. The above-mentioned fractions are all parts by weight. The drug is subdivided and mixed with starch, and starch slurry is added to make a soft material, granulated with a 14-mesh sieve, dried at 75°C, granulated with a 12-mesh sieve, and packaged to obtain the granules of this product.

Example 4

(1) Weigh each raw material according to the following weight ratio (take ten times the ratio of raw materials as an example)

600 parts of astragalus, 400 parts of psoralen, 300 parts of Ligustrum lucidum, 200 parts of wolfberry, and 150 parts of scrophulariaceae for later use.

(2) Astragalus, psoraleae, wolfberry, scrophulariaceae and Ligustrum lucidum in the above weight ratio are soaked in 10 times the amount of water for 1.5 hours, ultrasonically extracted for 20 minutes, filtered, and the filtrate is collected; the filter residue is added with 8 times the amount of water, and ultrasonically extracted After 15 minutes, filter and collect the filtrate; add 6 times the amount of water to the filter residue, and ultrasonically extract for 10 minutes. The three filtrates were combined, and the solvent was removed by rotary evaporation to make a dry extract, which was baked in an oven at 75°C until completely dry and then pulverized into fine powder (passed through a 80-mesh sieve).

(3) Dissolve 60g of the above-mentioned fine powder in 500ml of distilled water, stir to dissolve, filter, and add distilled water to 1000ml through the filter to obtain the oral liquid of this product.

Example 5

Influence of the Chinese medicine compound of the present invention on the metastasis inhibition rate of FC gastric cancer

Experimental materials: 615 mice, gastric cancer FC tumor strain.

Experimental methods and results: Under sterile conditions, a 1:3 dilution of FC cell suspension was prepared, with a cell concentration of 2×10 ⁶ /ml, and 0.2ml was subcutaneously injected into the axilla of each mouse. After 24 hours, they were randomly divided into 4 groups according to body weight: Control group and high, medium and low dose groups, 10 rats in each group, half male and half male.

The traditional Chinese medicine compound prescription of the present invention adopts the ultrasonic method to extract the active ingredient and then concentrates to the corresponding concentration. Different dose groups include high dose group (40g/kg), middle dose group (20g/kg) and low dose group (10g/kg). Oral administration once a day for 10 consecutive days. The control group was intragastrically given the same amount of normal saline. The mice were sacrificed on the day after drug withdrawal, weighed, the lungs and metastatic nodules were removed, and the inhibition rate of lung metastasis of gastric cancer was calculated. The results are shown in Table 1. From the results in Table 1, it can be seen that the Chinese medicine compound of the present invention has a good effect on inhibiting the metastasis of gastric cancer in mice with gastric cancer. The inhibition rate of low-dose drugs on the metastasis of FC gastric cancer is 50%, and the inhibition rate of high-dose drugs on the metastasis of FC gastric cancer is as high as 90%.

Example 6

Influence of the Chinese medicine compound of the present invention on tumor metastasis inhibition rate in FC gastric cancer mice

Experimental animals: inbred 615 mice, male, 6-8 weeks old, weighing 18-22 g.

Experimental methods and results: The transplantable forestomach squamous cell carcinoma (FC) tissues were taken 8-10 days after transplantation, prepared into a cell suspension of 1×10 ¹⁰ /L, and injected subcutaneously into the right axilla of mice 0.2ml/piece. After being inoculated with the tumor strain, the mice were randomly divided into 4 groups, including the control group and high, medium and low dose groups, with 10 mice in each group.

The traditional Chinese medicine compound prescription of the present invention adopts the ultrasonic method to extract the active ingredient and then concentrates to the corresponding concentration. Different dose groups include high dose group (40g/kg), middle dose group (20g/kg) and low dose group (10g/kg). Animals in each group were intragastrically administered on the second day after tumor inoculation, once a day for 3 consecutive weeks, and the control group was intragastrically administered with the same amount of normal saline. After the experiment, the experimental mice were dissected, the lungs were taken out, and the total number of metastatic lesions in the lungs was counted. The metastases were beige, rounded nodules with smooth edges. Metastasis inhibition rate=(the number of metastases in the blank group-the number of metastases in the drug-administered group)/the number of metastases in the blank group×100%. The results are shown in Table 2. From the results in Table 2, it can be seen that the Chinese medicine compound of the present invention has a good effect on inhibiting the metastasis of gastric cancer in mice with gastric cancer. With the increase of drug dose, the number of metastatic tumor nodules decreased. The inhibition rate of tumor metastasis in FC gastric cancer mice was 20.5% for low-dose drugs, and 54.5% for high-dose drugs.

Table 1 The results of the influence of traditional Chinese medicine compound on the metastasis inhibition rate of FC gastric cancer

group Dose (g/kg) number of animals Number of transfers Transfer rate (%) transfer inhibition rate

Control group Low dose group Middle dose group High dose group 0102040   10101010 10/105/104/101/10   100504010 -506090

Table 2 Effects of traditional Chinese medicine compound on tumor metastasis inhibition rate in FC gastric cancer mice

grouping Dose (g/kg) number of animals Number of tumor nodules (pieces) Transfer inhibition rate (%)   Control group, low dose group, middle dose group, high dose group 0102040   10101010 15.6±2.612.4±2.110.2±1.97.1±1.7 -20.534.654.5

The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplifications should be equivalent replacement methods, and all are included in the protection scope of the present invention.

Claims (6)

1. the compound Chinese medicinal preparation of an anti-stomach cancer metastasis, it is characterized in that: the raw material of Chinese medicine by following weight parts is made: 40～70 parts of the Radixs Astragali, 30～60 parts of Fructus Psoraleaes, 20～40 parts of Fructus Ligustri Lucidi, 20～40 parts of Fructus Lyciis, 10～30 parts of Radix Scrophulariaes.

2. the compound Chinese medicinal preparation of anti-stomach cancer metastasis according to claim 1, it is characterized in that: the raw material of Chinese medicine by following weight parts is made: 55 parts of the Radixs Astragali, 50 parts of Fructus Psoraleaes, 30 parts of Fructus Ligustri Lucidi, 30 parts of Fructus Lyciis, 20 parts of Radix Scrophulariaes.

3. the compound Chinese medicinal preparation of anti-stomach cancer metastasis according to claim 1 is characterized in that: the conventional oral formulations of its dosage form for adopting the Chinese medicine preparation conventional method to be prepared into.

4. the compound Chinese medicinal preparation of anti-stomach cancer metastasis according to claim 3, it is characterized in that: described conventional oral formulations is tablet, capsule, oral liquid or electuary.

5. the preparation method of the compound Chinese medicinal preparation of anti-stomach cancer metastasis according to claim 1 is characterized in that comprising the steps:

(1) Radix Astragali, Fructus Psoraleae, Fructus Lycii, Radix Scrophulariae and Fructus Ligustri Lucidi are mixed by described weight ratio, add 8～12 times of water gagings and soaked 1～2 hour, supersound extraction 10～30 minutes is filtered, and collects filtrate; Filtering residue adds 6～10 times of water gagings, and supersound extraction 10～20 minutes is filtered, and collects filtrate; Filtering residue adds 4～8 times of water gagings, and supersound extraction 5～15 minutes is filtered, and collects filtrate; Merge three times filtrate, rotary evaporation is flung to solvent and is made dry extract, and 70～80 ℃ are ground into fine powder after being baked to bone dry in baking oven;

The various conventional adjuvant that needs when (2) this fine powder being added the preparation different dosage form, method of Chinese medicinal routinely is prepared into required Chinese medicine preparation dosage form.

6. the preparation method of the compound Chinese medicinal preparation of anti-stomach cancer metastasis according to claim 5, it is characterized in that: in the described step (1) with the mixing of the Radix Astragali, Fructus Psoraleae, Fructus Lycii, Radix Scrophulariae and Fructus Ligustri Lucidi according to described weight ratio, adding 10 times of water gagings soaked 1.5 hours, supersound extraction 20 minutes, filter, collect filtrate; Filtering residue adds 8 times of water gagings, and supersound extraction 15 minutes is filtered, and collects filtrate; Filtering residue adds 6 times of water gagings, and supersound extraction 10 minutes is filtered, and collects filtrate; Merge three times filtrate, rotary evaporation is flung to solvent and is made dry extract, and 70～80 ℃ are ground into fine powder after being baked to bone dry in baking oven.