CN101815784A - 植物系统中外来核酸和多肽的产生 - Google Patents

植物系统中外来核酸和多肽的产生 Download PDF

Info

Publication number
CN101815784A
CN101815784A CN200780007805A CN200780007805A CN101815784A CN 101815784 A CN101815784 A CN 101815784A CN 200780007805 A CN200780007805 A CN 200780007805A CN 200780007805 A CN200780007805 A CN 200780007805A CN 101815784 A CN101815784 A CN 101815784A
Authority
CN
China
Prior art keywords
protein
polypeptide
plant
plants
seedlings
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200780007805A
Other languages
English (en)
Chinese (zh)
Inventor
维达季·尤西波夫
瓦季姆·梅特
莎伊拉贾·拉宾德拉
穆奈姆·沙劳尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer USA Inc
Original Assignee
Fraunhofer USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/353,905 external-priority patent/US7692063B2/en
Application filed by Fraunhofer USA Inc filed Critical Fraunhofer USA Inc
Publication of CN101815784A publication Critical patent/CN101815784A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A61K2039/541—Mucosal route
    • A61K2039/542—Mucosal route oral/gastrointestinal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Diabetes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN200780007805A 2006-02-13 2007-02-13 植物系统中外来核酸和多肽的产生 Pending CN101815784A (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US77337806P 2006-02-13 2006-02-13
US77337406P 2006-02-13 2006-02-13
US77325506P 2006-02-13 2006-02-13
US60/773,255 2006-02-13
US11/353,905 US7692063B2 (en) 2002-11-12 2006-02-13 Production of foreign nucleic acids and polypeptides in sprout systems
US11/353,905 2006-02-13
US60/773,374 2006-02-13
US60/773,378 2006-02-13
US87945007P 2007-01-09 2007-01-09
US60/879,450 2007-01-09
PCT/US2007/003942 WO2007095304A2 (en) 2006-02-13 2007-02-13 Production of foreign nucleic acids and polypeptides in plant systems

Publications (1)

Publication Number Publication Date
CN101815784A true CN101815784A (zh) 2010-08-25

Family

ID=38372119

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200780007805A Pending CN101815784A (zh) 2006-02-13 2007-02-13 植物系统中外来核酸和多肽的产生

Country Status (8)

Country Link
EP (1) EP1984509A4 (pt)
JP (1) JP2009528025A (pt)
KR (1) KR20080113372A (pt)
CN (1) CN101815784A (pt)
AU (1) AU2007215066A1 (pt)
BR (1) BRPI0707785A2 (pt)
CA (1) CA2642138A1 (pt)
WO (1) WO2007095304A2 (pt)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103146736A (zh) * 2013-03-28 2013-06-12 福建农林大学 一种植物表达载体pGⅡ0229-GUS的构建及应用
CN110423746A (zh) * 2019-07-16 2019-11-08 北京林业大学 编码小肽pfer的基因序列、小肽pfer和应用及诱导植物生根的方法
CN114736920A (zh) * 2022-03-01 2022-07-12 中国计量大学 农杆菌侵染菜豆叶片实现目的基因瞬时过表达或瞬时沉默的方法

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
CN1833030B (zh) 2003-05-22 2014-07-23 美国弗劳恩霍夫股份有限公司 用于表达、传递及纯化目标多肽的重组载体分子
EP1919504B1 (en) 2005-08-03 2013-10-16 iBio, Inc. Antibody to bacillus anthracis protective antigen
US8404252B2 (en) 2007-07-11 2013-03-26 Fraunhofer Usa, Inc. Yersinia pestis antigens, vaccine compositions, and related methods
CA2729628A1 (en) * 2008-06-30 2010-01-07 Orf Liftaekni Hf Industrial plant-based production of animal-free recombinant proteins in defined environment
BRPI0919485A2 (pt) * 2008-09-28 2015-12-01 Fraunhofer Usa Inc vacinas, antígenos, composições de plasmodium e métodos
WO2010037046A1 (en) 2008-09-28 2010-04-01 Fraunhofer Usa, Inc. Humanized neuraminidase antibody and methods of use thereof
US8784819B2 (en) 2009-09-29 2014-07-22 Ibio Inc. Influenza hemagglutinin antibodies, compositions and related methods
JP6358257B2 (ja) 2013-09-06 2018-07-18 三菱ケミカル株式会社 植物を用いたタンパク質の製造方法
CA2944422A1 (en) * 2014-04-10 2015-10-15 Mitsubishi Chemical Corporation Method for producing a useful protein using a plant
ES2876036T3 (es) * 2015-01-30 2021-11-11 Univ Tokyo Composición que contiene una bacteria láctica, composición farmacéutica oral para su uso en el tratamiento de infecciones por VPH y/o tumores asociados a VPH y agente inductor de inmunidad de la mucosa para su uso en dotar de inmunidad mucosal contra la infección por VPH
JP6578681B2 (ja) * 2015-03-11 2019-09-25 三菱ケミカル株式会社 植物栽培方法及びそれを用いる有用タンパク質の製造
JP7165056B2 (ja) * 2016-11-30 2022-11-02 キリンホールディングス株式会社 植物による有用タンパク質の生産方法
WO2018144843A1 (en) 2017-02-03 2018-08-09 Arizona Board Of Regents On Behalf Of The University Of Arizona Engineering high-protein-content soybeans
WO2018232406A1 (en) * 2017-06-16 2018-12-20 Arizona Board Of Regents On Behalf Of The University Of Arizona Methods, systems, and compositions for legume-based production of therapeutic proteins and therapeutic medical materials.
US10889826B2 (en) 2017-06-16 2021-01-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Methods and compositions for producing epidermal growth factor (EGF) in soybeans
EP3864042A1 (en) * 2018-10-11 2021-08-18 Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. Means and methods for detecting soy allergens
CN118085045B (zh) * 2023-11-29 2025-12-19 玉溪中烟种子有限责任公司 一种促进烟草生长的NtSSP2多肽及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693506A (en) * 1993-11-16 1997-12-02 The Regents Of The University Of California Process for protein production in plants
US6700040B2 (en) * 1998-01-16 2004-03-02 Large Scale Biology Corporation Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector
US7683238B2 (en) * 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
CN1833030B (zh) * 2003-05-22 2014-07-23 美国弗劳恩霍夫股份有限公司 用于表达、传递及纯化目标多肽的重组载体分子
DE602004027403D1 (de) * 2003-11-10 2010-07-08 Icon Genetics Ag Von rna-virus abgeleitetes pflanzenexpressionssystem
ES2532609T3 (es) * 2004-02-20 2015-03-30 Ibio, Inc. Sistemas y métodos para expresión clonales en plantas
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103146736A (zh) * 2013-03-28 2013-06-12 福建农林大学 一种植物表达载体pGⅡ0229-GUS的构建及应用
CN110423746A (zh) * 2019-07-16 2019-11-08 北京林业大学 编码小肽pfer的基因序列、小肽pfer和应用及诱导植物生根的方法
CN110423746B (zh) * 2019-07-16 2021-03-12 北京林业大学 编码小肽pfer的基因序列、小肽pfer和应用及诱导植物生根的方法
CN114736920A (zh) * 2022-03-01 2022-07-12 中国计量大学 农杆菌侵染菜豆叶片实现目的基因瞬时过表达或瞬时沉默的方法

Also Published As

Publication number Publication date
CA2642138A1 (en) 2007-08-23
EP1984509A4 (en) 2010-07-14
EP1984509A2 (en) 2008-10-29
AU2007215066A1 (en) 2007-08-23
JP2009528025A (ja) 2009-08-06
WO2007095304A3 (en) 2010-01-21
BRPI0707785A2 (pt) 2011-05-10
WO2007095304A2 (en) 2007-08-23
KR20080113372A (ko) 2008-12-30

Similar Documents

Publication Publication Date Title
US7683238B2 (en) Production of pharmaceutically active proteins in sprouted seedlings
WO2007095304A2 (en) Production of foreign nucleic acids and polypeptides in plant systems
Gao et al. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg
Birch‐Machin et al. Accumulation of rotavirus VP6 protein in chloroplasts of transplastomic tobacco is limited by protein stability
US8058511B2 (en) System for expression of genes in plants
US8148608B2 (en) Systems and methods for clonal expression in plants
US20110070609A1 (en) Production of Foreign Nucleic Acids and Polypeptides in Sprout Systems
KR20010085901A (ko) 식물에서의 치료학적 활성 단백질
CN111565747B (zh) 与猪fc片段融合的抗原，以及包含该抗原的疫苗组合物
KR20140034139A (ko) 식물에서의 단백질 발현
KR20110009197A (ko) 세균 독소 백신
TW200914611A (en) Pig edema disease vaccine
KR20080038131A (ko) 생물학적으로 활성인 거대분자의 점막 또는 장관 투여
US20080115243A1 (en) Transgenic Plants Expressing Intein Modified Proteins and Associated Processes for Bio-Pharmaceutical Production
CA2509678A1 (en) Immunization of fish with plant-expressed recombinant proteins
WO2007089950A2 (en) Preparations of growth hormone
Elkholy et al. Expression of hepatitis B surface antigen (HBsAg) gene in transgenic banana (Musa Sp.)
Wiktorek-Smagur et al. Green way of biomedicine–how to force plants to produce new important proteins
Cheung et al. Expression and subcellular targeting of human insulin-like growth factor binding protein-3 in transgenic tobacco plants
KR101428843B1 (ko) 로타바이러스에 대한 백신효과를 유도하는 형질전환체 및 이를 포함하는 백신 조성물
Alli et al. Pharming vaccines for hepatitis and cytomegalovirus: towards the development of multivalent and subunit vaccines for oral delivery of antigens
CN107573410A (zh) 一种人工合成的植物抗虫蛋白及其制备方法和应用
US20040237146A1 (en) Floral transformation
US20040268434A1 (en) Plant transformation
CZ20012644A3 (cs) Způsob provádění řízené produkce proteinů v hostitelském organismu po jeho zachycení

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20100825