CN106916723A - A kind of haematococcus pluvialis cultural method and culture apparatus - Google Patents

Abstract

The application is related to microalgae industry application field, in particular to a kind of haematococcus pluvialis cultural method and culture apparatus.More specifically, this application discloses a kind of stacked thin plate haematococcus pluvialis culture apparatus, it is characterized in that, the stacked thin plate haematococcus pluvialis culture apparatus is included in multiple thin plates of arranged stacked on vertical direction, the multiple thin plate is supported to cause to be spaced a preset distance between adjacent thin plate, each thin plate is horizontally extending, with opposite upper and lower surface, and it is provided peripherally with the cofferdam upwardly extended from the upper surface of corresponding thin plate, the upper surface of each thin plate and the inner surface of cofferdam form the space for accommodating haematococcus pluvialis nutrient solution, wherein described cofferdam is higher by the upper surface 1.5mm 4.5mm of corresponding thin plate.

Description

A kind of cultivation method and cultivation device of Haematococcus pluvialis

field of invention

The present application relates to the field of industrial application of microalgae, in particular to a cultivation method and cultivation device for Haematococcus pluvialis.

Background of the invention

Astaxanthin has been proven to improve the body's immunity, prevent ultraviolet (UV-A) damage to cells, inhibit the growth of cancer cells, delay the aging process, and prevent cardiovascular diseases, so it has great medical application prospects (Han D, Li Y, Hu Q. Astaxanthin in microalgae: pathways, functions and biotechnological implications. Algae, 2013, 28:131-147). In addition, astaxanthin is also the main component of the pigment of salmon (Salmon). For artificially farmed salmon, etc., it is necessary to add astaxanthin to the feed to ensure the bright red color of the fish. With the rapid development of this kind of economic aquatic animal breeding industry, the market demand for astaxanthin is also increasing rapidly. In 2013, sales of astaxanthin as a fish feed additive reached half a billion dollars.

Haematococcus pluvialis is the organism with the highest natural astaxanthin content. Under certain conditions, astaxanthin content can account for more than 1%-3% of the dry weight of Haematococcus pluvialis cells, so Haematococcus pluvialis is considered It is the best biological source of natural astaxanthin.

At present, the method of cultivating Haematococcus pluvialis mainly adopts the liquid immersion method, and typical representatives include open raceway pools and closed photobioreactors composed of flat-plate, pipe-type, column-type and other transparent containers. The immersion method is to immerse the algae in a large amount of medium, and keep the medium in a state of motion through agitator, circulation equipment or bubbling equipment, so that each algal cell can receive light and absorb CO ₂ evenly. However, these culture methods and culture devices have the disadvantages of large energy consumption, low cell density (the yield of cell dry weight is less than 1g/L), fast propagation speed inside the pollution source, low light utilization efficiency, large space occupation, large water consumption, and limited space per unit volume. Shortcomings such as low yield.

Another existing method for culturing Haematococcus pluvialis is the semi-dry method, that is, the algae are attached to the surface of sheet-shaped water-absorbing materials, and the attached algae grow like moss by supplying culture fluid to these water-absorbing materials. These water-absorbing materials can be hung vertically at certain intervals, and a light wall is placed on the side to provide light. The advantage of the semi-dry method is that there is no need to stir the culture solution, which can save energy compared with the immersion method; it occupies a small space, and many sheet-shaped water-absorbing materials can be hung in a unit space; and the algae cells are attached to the surface of the water-absorbing material, which can be directly used CO ₂ in the air, no need to artificially introduce CO ₂ . However, the surface of semi-dry water-absorbing materials (such as canvas, cotton, etc.) is very prone to mildew. This is because the growth environment of the mold is very consistent with the environment where the water-absorbing material membrane is located, and the growth rate of the mold even exceeds that of the algae. Once infected with mold, the entire absorbent material will deteriorate and even rot and break within a period of time. There is currently no mold treatment that does not leave a toxic residue on the algae. In addition, the semi-dry culture method needs to continuously or intermittently pump the culture solution to the top of the water-absorbing material and spray it down, which also requires a lot of energy consumption.

Therefore, there is still a need in the art for new methods and devices for cultivating Haematococcus pluvialis.

Contents of the invention

In one aspect, the present invention provides a stacked thin-plate Haematococcus pluvialis culture device, comprising a plurality of thin plates stacked in the vertical direction, and the plurality of thin plates are supported so that adjacent thin plates are spaced apart by For a predetermined distance, each thin plate extends horizontally, has opposite upper and lower surfaces, and is provided on the periphery with a cofferdam portion extending upward from the upper surface of the corresponding thin plate, and the upper surface of each thin plate and the inner surface of the cofferdam portion form The space for accommodating the culture solution of Haematococcus pluvialis, wherein the cofferdam part is 1.5mm-4.5mm higher than the upper surface of the corresponding thin plate.

In some embodiments, a plurality of supporting columns are arranged between adjacent thin plates in the vertical direction, and the supporting columns are used to support the upper thin plates.

In some embodiments, the dam portion is integrally formed with the corresponding sheet, or is formed separately and then attached to the corresponding sheet.

In some embodiments, the support posts are integrally formed with the corresponding sheet, or are formed separately and then attached to the lower surface of the corresponding sheet.

In some embodiments, the dam portion has an inclination angle greater than or equal to 90 degrees with respect to the upper surface of the corresponding sheet.

In some embodiments, the dam portion is made of the same or a different material than the sheet, and/or the support columns are made of the same or a different material as the sheet.

In some embodiments, the support column is in the form of a column or tube having a circular, rectangular or square cross-section.

In some embodiments, the thin plate is made of light-transmitting glass or plastic with high light-transmitting rate.

In some embodiments, the sheet is made of one of the following materials: glass, GPPS, transparent ABS, AS (styrene acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate ), PS (polystyrene).

In some embodiments, the thin plate is made of flat glass, and the coffering portion is an adhesive strip attached to the thin plate, preferably a transparent adhesive strip, that is, a circle of transparent adhesive strip is bonded around the flat glass A storage space for the culture medium is formed.

In some embodiments, the sheet is made of organic material, and the dam part is integrally formed with the sheet.

In some embodiments, the spacing between the thin plates in the vertical direction is the same or different.

In some embodiments, the spacing between adjacent sheets is 4.5-9.5mm or 10.5mm-15.5mm.

In some embodiments, the specification of the sheet is 12 cm in length and 6 cm in width.

In some embodiments, holes through which light can pass are formed on the supporting columns.

In some embodiments, a light source is provided on the side of the Haematococcus pluvialis cultivation device.

In another aspect, the present invention provides a method for cultivating Haematococcus pluvialis, which includes statically cultivating the Haematococcus pluvialis in a thin-layer liquid medium with a thickness of 0.5mm-2.5mm. In some embodiments, the Haematococcus pluvialis is Haematococcus pluvialis K-0084 strain.

In some embodiments, the ambient temperature used in the cultivation of Haematococcus pluvialis is 22-28°C.

In some embodiments, the light intensity used in the cultivation of Haematococcus pluvialis is 1-600umol·m ^-2 ·s ^-1 .

In some embodiments, the medium used in the cultivation of Haematococcus pluvialis is BG11 medium.

In some embodiments, the ambient humidity in the cultivation of Haematococcus pluvialis is in the range of 80-99%.

In some embodiments, the initial inoculation density of Haematococcus pluvialis in the culture of Haematococcus pluvialis is 0.1-0.3 OD ₇₅₀ .

In some embodiments, the culture of Haematococcus pluvialis is carried out in the stacked thin-plate Haematococcus pluvialis culture device of the present invention. In some embodiments, multiple stacked thin-plate Haematococcus pluvialis culture devices can be arranged in an array in the horizontal direction, wherein a light source is arranged on one side of the array.

Brief description of the drawings

Fig. 1 is a perspective view of a laminated thin-plate Haematococcus pluvialis cultivation device according to a preferred embodiment of the present invention;

Figure 2 is a front view of Figure 1; and

Fig. 3 is a sectional view along A-A in Fig. 2 .

Fig. 4A is a top view of the device array of the present invention, in which a light source of 1 m x 1 m is arranged on the left side, and 10 rows of devices of the present invention are arranged along the direction of light propagation.

Fig. 4B is a top view of a conventional flat container reactor, in which a 1 m x 1 m light source is set on the left side.

Fig. 5 shows the light intensity values on the medium surface of each row of the device array of the present invention and the final harvested biomass of Haematococcus pluvialis under different support column heights.

Fig. 6 shows the light source yield per unit area of Haematococcus pluvialis under different support column heights.

Detailed description of the invention

The invention provides a method for cultivating Haematococcus pluvialis, which comprises statically cultivating the Haematococcus pluvialls in a thin-layer liquid culture medium with a thickness of 0.5mm-2.5mm. In some embodiments, the Haematococcus pluvialis is Haematococcus pluvialis K-0084 strain obtained from the Scandinavian Culture Collection of Algaeand Protozoa, University of Copenhagen, Denmark.

Because the thickness of the culture medium is small enough (0.5mm-2.5mm) in the method of the present invention, therefore do not need the homogenization operation such as stirring and mixing, still can guarantee that the Haematococcus pluvialls cells in the culture fluid can obtain light more evenly (within the effective distance of light transmission to the underwater). And by using the culture medium thickness (0.5mm-2.5mm) described in the present invention, make Haematococcus pluvialis cell can directly obtain the _CO needed for photosynthesis from the liquid surface of culture solution by gas exchange and discharge to photosynthesis Inhibited _O2 is generated, so aeration of the culture medium is not required.

Harvesting and drying algae is an important step in algae cultivation, which consumes a lot of energy and has low efficiency. However, according to the method of the present invention, Haematococcus pluvialis is statically cultured using a thin-layer liquid medium, and the medium is usually nearly completely consumed when the algae grows to maturity (generally about 8-10 days). At this time, the dry weight of the cells has reached a relatively high ratio, which is very conducive to the harvesting and drying of the algae. Within the thickness range of the medium of the present invention (0.5mm-2.5mm), the thickness of the medium can be further adjusted according to factors such as Haematococcus pluvialls inoculation amount, light intensity, humidity and temperature, so that the amount of the medium is just right. Available for algae to grow to maturity without too much surplus. In addition, since the algae are always in the thin-layer culture solution, it is less prone to mold.

The thickness of the culture medium used in the methods of the present invention can be about 0.5 mm, about 0.7 mm, about 0.9 mm, about 1 mm, about 1.5 mm, about 2 mm, about 2.5 mm, or a range between any of said values.

In some embodiments, the temperature used in the method for culturing Haematococcus pluvialis is 22-28°C. In some embodiments, the light intensity used in the cultivation of Haematococcus pluvialis is 1-600umol·m ^-2 ·s ^-1 . In some embodiments, the medium used in the cultivation of Haematococcus pluvialis is BG11 medium. In some embodiments, the ambient humidity in the cultivation of Haematococcus pluvialis is in the range of 80-99%. In some embodiments, the initial inoculation density of Haematococcus pluvialis in the culture of Haematococcus pluvialis is 0.1-0.3 OD ₇₅₀ , wherein OD ₇₅₀ is the absorbance measured at a wavelength of 750 nm. 0.1-0.3OD ₇₅₀ corresponds to the initial inoculated biomass of 0.16g·L ^-1 -0.54g·L ^-1 .

The method for culturing Haematococcus pluvialls of the present invention adopts the mode of thin-layer static cultivation, and its essence is a novel method for cultivating Haematococcus pluvialls between the immersion method and the semi-dry method. The advantage of reducing consumption, reducing the cost of cultivation, and reducing the risk of mold contamination.

In another aspect, the present invention also provides a stacked thin-plate Haematococcus pluvialis culture device, which includes a plurality of thin plates stacked in the vertical direction and arranged at predetermined intervals, and the plurality of thin plates extend in the horizontal direction , each thin plate has an upper surface for placing Haematococcus pluvialis culture solution and an opposite lower surface, and the periphery of each thin plate is provided with a cofferdam portion protruding from the corresponding thin plate and extending upwardly from the upper surface of the corresponding thin plate , the cofferdam portion is 1.5mm-4.5mm higher than the upper surface of the corresponding thin plate.

1 to 3 show a layered thin-plate Haematococcus pluvialis culture device 100 according to a preferred embodiment of the present invention.

It can be seen from the figure that the stacked thin-plate Haematococcus pluvialis cultivation device 100 includes a plurality of thin plates 20 stacked in the vertical direction and a plurality of support columns 40 between adjacent thin plates. Each sheet 20 extends horizontally and has an upper surface 22 for placing a culture solution of Haematococcus pluvialis and an opposite lower surface 24 . The periphery of each thin plate 20 is provided with a cofferdam portion 26 extending upward from the upper surface 22 of the corresponding thin plate 20 protruding from the corresponding thin plate 20 .

Each thin plate 20 forms a culture plate of the culture device, that is, the upper surface 22 of each thin plate 20 and the inner surface of the coffered portion 26 of the thin plate 20 define a space 30 for containing the culture solution of Haematococcus pluvialis.

The thin plate 20 forming the culture device can be a transparent flat plate made of glass material or organic material. Compared with tubular, trough-shaped, or tank-shaped culture devices, the Haematococcus pluvialis culture device made of flat plates according to the present invention is simple to manufacture, low in cost, and can achieve the same culture area as a single culture plate. Larger, the thickness can also be relatively thin, and the transparency can be higher. The length of the thin plate may be, for example, 10cm-10m, and the width may be, for example, 5cm-1m. In some embodiments, the specification of the sheet is 12 cm in length x 6 cm in width.

The thin plates 20 are stacked vertically so that the structure is integrated and the effective cultivation area per unit occupied space is maximized.

Because the material of each sheet 20 is a material with high transparency, at the initial stage of the cultivation of Haematococcus pluvialls, because the Haematococcus pluvialls on each sheet 20 has not yet formed a dark biofilm, so the light from the outside can pass through. The upper surface of each thin plate 20 reaches the culture liquid surface of the adjacent lower thin plate, and also can reach the culture liquid surface of the upper thin plate from the lower surface of each thin plate 20, so the light efficiency is higher. The light source directly irradiates the algae cells above from the upper surface of the thin plate 20 and irradiates the cells cultured on the upper surface 22 of the lower thin plate 20 through the thin plate 20, so the light receiving area is large and the light utilization efficiency is very high.

With the Haematococcus pluvialis cultivation device of the present structure, less water is used, so the cultivation of the Haematococcus pluvialls does not need stirring, which saves energy consumption. In addition, due to the small amount of water, the harvesting process can be reduced and the harvesting cost can be reduced. Therefore, using the Haematococcus pluvialis culture device of this structure to cultivate Haematococcus pluvialls is a static culture, and there is no need to transport gas or provide stirring power during the cultivation process, and it can obtain light well.

According to the present invention, the dam portion 26 of each sheet 20 may be integrally formed with the sheet 20 or may be formed separately and then attached to the sheet 20 .

When the thin plate 20 is made of glass material, the dam portion 26 may be a circle of adhesive strips, preferably transparent adhesive strips, bonded to the periphery of the thin plate 20 . For the thin plate 20 of organic material, the dam portion 26 can be integrally formed with the thin plate 20 .

The material that forms thin plate 20 can be the high plastics of glass or light transmittance, as GPPS, transparent ABS, AS (styrene acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene), etc. The material forming the dam portion 26 can be the same material as the thin plate 20, or other transparent materials, such as rubber strips, glass glue, etc. can also be used.

The cofferdam part 26 forms an angle greater than or equal to 90 degrees with the upper surface 22 of the thin plate 20, so that the Haematococcus pluvialis cultivated on the upper surface 22 is blown out of the thin plate 20 with high-pressure water/gas, thereby realizing pluvialls. Harvesting of Haematococcus; preferably, the cofferdam portion 26 forms an angle greater than 90 degrees with the upper surface 22 of the thin plate 20 and forms a rounded corner between the joints to prevent the formation of dead angles for harvesting or cleaning. Optionally, the distance of the dam portion 26 above the upper surface 22 of the corresponding thin plate 20 is 1.5 mm, 2.5 mm, 3.5 mm, 4.5 mm, or a range between any of the above-mentioned values. For example, the distance may be 1.5mm-4.5mm.

The spacing between the thin plates 20 (the vertical distance between the lower surfaces of two adjacent thin plates, or the vertical distance between the upper surfaces of two adjacent thin plates) is determined according to the arrangement of light sources, the cultivation and harvesting requirements of Haematococcus pluvialis. For example, the spacing between the thin plates 20 is 4.5mm, 5.5mm, 6.5mm, 7.5mm, 8.5mm, 9.5mm, 10.5mm, 11.5mm, 12.5mm, 13.5mm, 14.5mm or 15.5mm, or any value mentioned above The range between, such as 4.5-9.5mm or 10.5mm-15.5mm.

The shape, material, quantity, spacing and arrangement of the support columns 40 are not limited, as long as the support columns 40 have a certain strength enough to support the culture solution on the stacked thin plates 20 and their upper surfaces 22 . Preferably, the support column 40 is made of transparent materials, such as glass, acrylic, PC and other materials. The support column 40 can have any suitable shape, including but not limited to cylindrical, round tube, square, strip and so on.

In the illustrated embodiment, the support columns 40 are arranged in a row. Optionally, the support column 40 is provided with a hole for allowing light to pass through.

For the glass thin plate 20, the support column 40 can be a glass column or a non-glass column. The support column 40 can be separated from both the upper and lower sheets 20 , or can be bonded to the lower surface 24 of the upper sheet 20 .

For the thin plate 20 made of organic transparent material, the support column 40 can be integrally formed with the thin plate 20 .

The stacked thin-plate Haematococcus pluvialis culture device of the present invention can further be provided with a culture solution instillation device 50 capable of replenishing the culture solution to each thin plate 20, and the culture solution infusion device 50 is schematically shown in FIG. 3 , And optionally, liquid level monitors (not shown) that measure the precise liquid level of each sheet 20 may be provided. In addition, a light source 60 is shown in FIG. 3 . The light source can be arranged on one side of the stacked thin-plate Haematococcus pluvialis culture device of the present invention.

Optionally, the laminated thin-plate Haematococcus pluvialis culture device of the present invention may further include a light-transmitting film cover for enclosing the laminated thin-plate Haematococcus pluvialis culture device, which is the layered thin-plate Haematococcus pluvialis inside. The Haematococcus culture device provides an atmosphere with constant temperature and air humidity. This can reduce the evaporation of water and create the most suitable growth conditions for the cultivation of Haematococcus pluvialis, so that during the cultivation period of Haematococcus pluvialis, there is no need to replenish the supply of culture fluid.

In addition to the laminated thin-plate Haematococcus pluvialis cultivation device of the above-mentioned example, each thin plate 20 can also be placed on a support with several cross bars respectively, and the gravity of each thin plate 20 and the culture solution can be dispersed by this support; The same technical effect as the foregoing examples can be achieved.

The aforementioned method for culturing Haematococcus pluvialls of the present invention can be carried out in the laminated thin-plate Haematococcus pluvialls cultivation device of the present invention, so that it can be scaled up and cultured Haematococcus pluvialls at low cost.

In some embodiments of the method for cultivating Haematococcus pluvialis, multiple stacked thin-plate Haematococcus pluvialis cultivating devices may be arranged in an array in the horizontal direction. In some embodiments, a light source is disposed on one side of the array. A plurality of culture devices are horizontally arranged in an array, and because the cofferdam part separates each thin plate, the possible pollution in the culture can be limited to a smaller range.

Use the culture device of the present invention to carry out high-density layered culture of Haematococcus pluvialis. Since there is a gap between each layer, light can be transmitted to a long distance, so it can be used for culture within the reach of light. Haematococcus pluvialis. Therefore, the biomass output per unit area of the light source is directly increased, and the utilization rate of light energy is greatly improved.

Use the device of the present invention to cultivate Haematococcus pluvialis, assuming that the layer spacing is 4.9mm-11.9mm, and the thickness of the culture solution is 0.5mm-2.5mm, then 84-204 layers can be stacked in a space of 1m height. If Haematococcus pluvialis is cultivated in the cofferdam of each layer of thin plates, the biomass that can be cultured within the height range of 1m is quite considerable. In the following examples of the present invention, the dry cell weight can reach 416g/m ³ , the net increase can reach 309g/m ³ , the net yield per unit light source area can reach 29.4-30.9g/m ² /d, and the pluvialis red ball per unit mass The energy consumption of algae is only about 213-405 kJ/g.

Example

In order to further demonstrate the beneficial technical effects of the cultivation method and cultivation device of the present invention, the following examples will discuss "production per unit volume", "net production rate of light source per unit area", "energy consumption per unit mass of algae flour", "water consumption per unit mass of algae flour" etc. factors were examined.

test group:

Haematococcus pluvialis K-0084 strain (also named CW316) was cultured using the culture device of the present invention arranged in an array. A top view of the array is shown in Figure 4A.

On the left is a light source with a length of 1m and a height of 1m, with a light intensity of 600umol·m ^-2 ·s ^-1 . On the right is an array of several culture devices of the present invention. Ten rows of culture devices are arranged in the direction perpendicular to the light source. Among them, each thin plate (glass plate) has a specification of 120mm×60mm, and the layer spacing is 11.9mm, 6.8mm, and 4.9mm respectively, and is stacked up to 1m in the vertical direction. The height of the cofferdam is about 1.5 mm. Each sheet is covered with culture medium of Haematococcus pluvialis. The amount of algae liquid that can be accommodated on each sheet surface is about 10ml. The ambient temperature is controlled at about 25 degrees, and the relative humidity is 80%-99%. The initial inoculum OD ₇₅₀ on each thin plate was 0.2, corresponding to an inoculum volume of 0.35 g·L ^-1 . The cultivation was carried out in an environment completely shielded from natural light to exclude interference from natural light.

As detected by the light intensity detector, the light intensity on the surface of the medium in the culture device from the 10th row farthest to the light source to the 1st row closest to the light source is shown in FIG. 5 . It can be seen from Figure 5 that due to the support columns between the stacked thin plates, there are a large number of small spaces between the layers, and the light can be continuously transmitted to the far side through the gaps between the layers, and the farther away the light intensity is weaker. . From left to right in each figure in Fig. 5 are the light intensity values on the surface of the culture medium in the 10th row, the 9th row ... the 1st row and the biomass of Haematococcus pluvialis finally harvested in each row.

After observation, the growth condition of the glass thin plates in the first row is the best because it is close to the light source, the further away from the light source the worse the growth condition of the lamp panels, and the growth condition of the 10th row is the worst. Harvest after about 6 days of cultivation, count the total biomass of Haematococcus pluvialis in each row, and the growth situation is shown in Figure 5. It can be seen from this that although the growth of Haematococcus pluvialis in the 10th row is relatively poor, it can still obtain light and grow. The number of rows arranged in the direction perpendicular to the light source can be adjusted according to the intensity of the light source to obtain suitable growth of Haematococcus pluvialis.

Control group:

The cultivation of Haematococcus pluvialis using a traditional flat-plate container reactor (goldfish tank reactor) was carried out in an environment completely shielded from natural light. As shown in the top view of Fig. 4B, a light source of 1 m 2 illuminates one side of a conventional flat container type reactor, the side of the flat container is 1 m 2 , and the container width is 5 cm. Traditional flat vessel reactors require stirring and dedicated _CO2 high-pressure tank gas supply.

The light intensity measured after the light passed through the 5cm algae liquid was almost 0, and the light could not spread further, resulting in a light source of 1m2 only being able to illuminate the growth of Haematococcus pluvialis in a row of flat containers. The volume of the flat container is 1㎡*0.05m=50L.

Result comparison:

As mentioned above, the results of cultivating Haematococcus pluvialls using the laminated thin-plate Haematococcus pluvialls cultivation device of the present invention and the traditional flat container type reactor are summarized in the following table 1 (all with 1㎡ light source as reference object):

Table 1

this invention Flat Photobioreactor Yield per unit volume 1.01g/L 0.73g/L Net yield of light source per unit area Water consumption per unit mass of algae flour 0.99L/g 1.37L/g Energy consumption per unit mass of algae flour 213-405kJ/g 1717kJ/g

Figure 6 shows that when the interlayer spacing is 11.9mm, 6.8mm, and 4.9mm, the light source yields per unit area using the device of the present invention are 10.83g/m ² /d, 15.44g/m ² /d, and 19.46g/m ² respectively /d. It can be seen that using the culture device of the present invention, under the above layer spacing, the yield per unit area is higher than that of traditional flat-plate containers. However, if the spacing is too small, the algae liquid will easily stick to the upper glass sheet, which will affect the growth of Haematococcus pluvialis. The proper spacing should not be less than 4.5mm.

It can be seen that the method and device for cultivating Haematococcus pluvialis of the present invention are significantly better than the methods and devices of the prior art. In addition, the cultivation method and device of the present invention are cultivated in a static manner, during which there is no need for high-pressure _CO2 to realize air flotation stirring, and no need for circulating pumps, stirring paddles and other equipment, which further reduces energy consumption. In addition, the present invention uses thin-layer culture, and the harvested Haematococcus pluvialis is already in a semi-humid state, further reducing the energy consumption of concentration and drying.

Claims (24)

1. A stacked thin-plate Haematococcus pluvialis culture device, characterized in that, the stacked thin-plate Haematococcus pluvialis culture device comprises a plurality of thin plates stacked in the vertical direction, and the plurality of thin plates are The support makes a predetermined distance between adjacent thin plates, each thin plate extends in the horizontal direction, has opposite upper and lower surfaces, and is provided with a cofferdam portion extending upward from the upper surface of the corresponding thin plate on the periphery, each thin plate The upper surface and the inner surface of the dam part form a space for accommodating the culture solution of Haematococcus pluvialis, wherein the dam part is 1.5mm-4.5mm higher than the upper surface of the corresponding thin plate. 2. The stacked thin-plate Haematococcus pluvialis culture device according to claim 1, characterized in that a plurality of supporting columns are arranged between adjacent thin plates in the vertical direction, and the supporting columns are used to support the upper thin plates. 3. The laminated thin-plate Haematococcus pluvialis culture device according to claim 1 or 2, characterized in that the dam portion is formed integrally with the corresponding thin plate, or is attached to the corresponding thin plate after being formed separately. 4. The laminated thin-plate Haematococcus pluvialis culture device according to any one of claims 1-3, characterized in that, the support column is integrally formed with the corresponding thin plate, or is attached to the bottom of the corresponding thin plate after being formed separately surface. 5. The stacked thin-plate Haematococcus pluvialis culture device according to any one of claims 1-4, characterized in that, the cofferdam part has an inclination angle greater than or equal to 90 degrees with respect to the upper surface of the corresponding thin plate. 6. The stacked thin-plate Haematococcus pluvialis culture device according to any one of claims 1-5, characterized in that, the cofferdam part is made of the same or different material as the thin plate, and/or the support column Made of the same or different material as the sheet. 7. The stacked thin-plate Haematococcus pluvialis culture device according to any one of claims 1-6, characterized in that, the support column is in the form of a column or tube with a circular, rectangular or square cross-section. 8. The stacked thin-plate Haematococcus pluvialis culture device according to any one of claims 1-7, characterized in that said thin plate is made of light-transmitting glass or plastic with high light-transmitting rate. 9. The stacked thin-plate Haematococcus pluvialis cultivation device according to any one of claims 1-8, wherein said thin plate is made of one of the following materials: glass, GPPS, transparent ABS, AS (styrene Acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene). 10. The stacked thin-plate Haematococcus pluvialis cultivation device according to claim 1, characterized in that, the thin plate is made of flat glass, and the cofferdam part is an adhesive strip attached to the thin plate, preferably a transparent adhesive strip . 11. The stacked thin-plate Haematococcus pluvialis culture device according to claim 1, characterized in that the thin plate is made of organic material, and the cofferdam part is integrally formed with the thin plate. 12. The stacked thin-plate Haematococcus pluvialis culture device according to any one of claims 1-11, characterized in that the distances between the thin plates are the same or different. 13. The stacked thin-plate Haematococcus pluvialis cultivation device according to any one of claims 1-12, characterized in that the distance between adjacent thin plates is 4.5mm-9.5mm or 10.5mm-15.5mm. 14. The stacked thin-plate Haematococcus pluvialis culture device according to any one of claims 1-13, characterized in that, the specification of the thin plate is 12 cm in length and 6 cm in width. 15. The laminated thin-plate Haematococcus pluvialis culture device according to any one of claims 1-14, characterized in that holes through which light can pass are formed on the support columns. 16. A method for cultivating Haematococcus pluvialis, comprising statically cultivating the Haematococcus pluvialls in a thin-layer liquid medium with a thickness of 0.5mm-2.5mm. 17. The method of claim 16, wherein said Haematococcus pluvialis is Haematococcus pluvialis K-0084 strain. 18. The method of claim 16 or 17, wherein said culturing is performed at 20-30°C. 19. The method according to any one of claims 16-18, wherein the culturing is carried out under a light intensity of 1-600 umol·m ^-2 ·s ^-1 . 20. The method of any one of claims 16-19, wherein the medium is BG11 medium. 21. The method of any one of claims 16-20, wherein the culturing is performed at a humidity of 80-99%. 22. The method of any one of claims 16-21, wherein the density of the initial inoculation of Haematococcus pluvialis is 0.1-0.3 _OD750 . 23. The method according to any one of claims 16-22, wherein the cultivation of Haematococcus pluvialis is carried out in the stacked thin-plate Haematococcus pluvialis cultivation device according to any one of claims 1-15. 24. The method of claim 23, wherein a plurality of stacked thin-plate Haematococcus pluvialis cultivation devices are arranged in an array in the horizontal direction, wherein a light source is arranged on one side of the array.