CN106916723B - A kind of haematococcus pluvialis cultural method and culture apparatus - Google Patents
A kind of haematococcus pluvialis cultural method and culture apparatus Download PDFInfo
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Abstract
This application involves microalgae industry application fields, in particular to a kind of haematococcus pluvialis cultural method and culture apparatus.More specifically, this application discloses a kind of stacked thin plate haematococcus pluvialis culture apparatus, it is characterized in that, the stacked thin plate haematococcus pluvialis culture apparatus includes multiple thin plates of the arranged stacked on vertical direction, the multiple thin plate is supported so that being spaced a preset distance between adjacent thin plate, each thin plate is horizontally extending, with opposite upper and lower surfaces, and it is provided peripherally with the cofferdam upwardly extended from the upper surface of corresponding thin plate, the upper surface of each thin plate and the inner surface of cofferdam form the space for accommodating haematococcus pluvialis culture solution, wherein the cofferdam is higher by the upper surface 1.5mm-4.5mm of corresponding thin plate.
Description
Invention field
This application involves microalgae industry application fields, in particular to a kind of haematococcus pluvialis (Haematococcus
Pluvialis) cultural method and culture apparatus.
Background of invention
Astaxanthin prevents damage of the ultraviolet light (UV-A) to cell it is verified that immunity of organisms can be improved, and inhibits
Growth of cancer cells, retardation aging course, prevent cardiovascular disease, therefore have huge medical applications prospect (Han D, Li Y,
Hu Q.Astaxanthin in microalgae:pathways,functions and biotechnological
implications.Algae,2013,28:131-147).In addition, astaxanthin is also the master of the pigment of dog salmon (Salmon)
Want composition.For the dog salmon etc. of artificial breeding, need to add astaxanthin in feed to guarantee the scarlet color of the flesh of fish.With
The rapid development of this kind of economy aquatic animals aquaculture, market the demand of astaxanthin is also increased rapidly.2013, shrimp was green
The plain sales volume as fish feed additive is up to 500,000,000 dollars.
Haematococcus pluvialis is the highest biology of natural astaxanthin content, and under given conditions, content astaxanthin can Zhan Yusheng
The 1%-3% or more of haematococcus dry cell weight, therefore haematococcus pluvialis is considered as the best biological source of natural astaxanthin.
The method of culture haematococcus pluvialis mainly uses liquid-immersed method at present, it is typical represent have open raceway pond with
And the closed photo bioreactor of the compositions such as other transparent vessels such as flat, duct type, column.Immersion method is by algae
It is immersed in a large amount of culture medium, so that culture medium is continuously in motion state by blender or recycle unit or sparging apparatus,
Make each frustule that can equably receive illumination and absorbs CO2.However, these cultural methods and culture apparatus have, energy consumption is high,
Cell density low (dry cell weight yield is less than 1g/L), pollution sources internal communication speed is fast, light utilization ratio is low, occupied space
Greatly, the disadvantages such as water consumption is big, unit volume space yield is low.
Existing another kind haematococcus pluvialis cultural method is semidry method, i.e., algae is attached to sheet water-absorbing material table
Face grows the algae of attachment equally like moss by supplying culture solution to these water-absorbing materials.These water-absorbing materials can
It is hung vertically with a fixed spacing, and is being laterally located a smooth wall to provide illumination.The benefit of semidry method is to be not necessarily to stir culture liquid,
Opposite immersion method can save energy consumption；It occupies little space, many sheet water-absorbing materials can be hung in a unit space；
And frustule is attached to water-absorbing material surface, can directly utilize the CO in air2, without being manually passed through CO2.However, semidry method
Water-absorbent material (such as canvas, cotton) surface be very easy to mildew.This is because the growing environment and water-absorbing material of mould
Environment where diaphragm coincide very much, and the mould speed of growth is even more than the speed of algae.Once catching mould, monolith water imbibition material
It is even rotten broken that rotten denaturation can all occur whithin a period of time for material.Currently without to the remaining mould processing side of algae nonhazardous
Method.In addition, semidry method training method needs continuously or discontinuously to spray culture liquid pump down to water-absorbing material top, also have
No small energy requirements.
Therefore, this field still needs new haematococcus pluvialis cultural method and device.
Summary of the invention
On the one hand, the present invention provides a kind of stacked thin plate haematococcus pluvialis culture apparatus, it is included in vertical direction
Multiple thin plates of upper arranged stacked, the multiple thin plate is supported so that be spaced a preset distance between adjacent thin plate, each thin
Plate is horizontally extending, have opposite upper and lower surfaces, and be provided peripherally with from the upper surface of corresponding thin plate to
The cofferdam of upper extension, the upper surface of each thin plate and the inner surface of cofferdam form the sky for accommodating haematococcus pluvialis culture solution
Between, wherein the cofferdam is higher by the upper surface 1.5mm-4.5mm of corresponding thin plate.
In some embodiments, multiple support columns are set between the adjacent thin plate of up and down direction, the support column is used for
The thin plate of support above.
In some embodiments, the cofferdam is integrally formed with corresponding thin plate, or after independently forming it is attached
It is connected to corresponding thin plate.
In some embodiments, the support column is integrally formed with corresponding thin plate, or after independently forming it is attached
It is connected to the lower surface of corresponding thin plate.
In some embodiments, the cofferdam is relative to the upper surface of corresponding thin plate at inclining more than or equal to 90 degree
Angle.
In some embodiments, the cofferdam is made and/or the support of the material identical or different with thin plate
Column is made of the material identical or different with thin plate.
In some embodiments, the support column is the shape of column or pipe with round, rectangle or square cross section
Formula.
In some embodiments, the thin plate is made of the high plastics of the glass or light transmittance of light transmission.
In some embodiments, the thin plate is made of one of materials described below: glass, GPPS, transparent ABS, AS (benzene
Ethylene, propylene nitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene).
In some embodiments, the thin plate is made of plate glass, and the cofferdam is attached to thin plate
Adhesive tape, preferably cellophane adhesive tape form the receiving of a culture solution in one circle cellophane adhesive tape of the surrounding bonding of the plate glass
Space.
In some embodiments, the thin plate is made of organic material, and the cofferdam and thin plate are integrally formed.
In some embodiments, the spacing between each thin plate of up and down direction is identical or different.
In some embodiments, the spacing between adjacent sheet is 4.5-9.5mm or 10.5mm-15.5mm.
In some embodiments, the specification of the thin plate is length 12cm × width 6cm.
In some embodiments, the hole that light can penetrate is formed on the support column.
In some embodiments, the side of the haematococcus pluvialis culture apparatus is provided with light source.
On the other hand, the present invention provides a kind of haematococcus pluvialis cultural methods, including with a thickness of 0.5mm-
Haematococcus pluvialis described in the thin layer fluid nutrient medium static culture of 2.5mm.In some embodiments, the haematococcus pluvialis is
Haematococcus pluvialis K-0084 algae strain.
In some embodiments, environment temperature used in the haematococcus pluvialis culture is 22-28 DEG C.
In some embodiments, light intensity used in the haematococcus pluvialis culture is 1-600umolm-2·s-1。
In some embodiments, culture medium used in the haematococcus pluvialis culture is BG11 culture medium.
In some embodiments, the ambient humidity range in the haematococcus pluvialis culture is 80-99%.
In some embodiments, the density of haematococcus pluvialis initial inoculation is 0.1- in the haematococcus pluvialis culture
0.3OD750。
In some embodiments, the haematococcus pluvialis culture is in stacked thin plate haematococcus pluvialis culture of the invention
It is carried out in device.In some embodiments, multiple stacked thin plate haematococcus pluvialis culture apparatus can in the horizontal direction by
It is arranged to array, wherein the side in array is disposed with light source.
Brief description
Fig. 1 is the perspective view of stacked thin plate haematococcus pluvialis culture apparatus according to the preferred embodiment of the invention；
Fig. 2 is the front view of Fig. 1；And
Fig. 3 is the cross-sectional view of the A-A along Fig. 2.
Fig. 4 A is the top view of apparatus of the present invention array, wherein the light source of left side setting 1m × 1m, along the light direction of propagation
10 row's the device of the invention are set.
Fig. 4 B is the top view of classic flat-plate vessel type reactor, wherein the light source of left side setting 1m × 1m.
Fig. 5 shows apparatus of the present invention array under different support column heights and respectively arranges the light intensity value of media surface and finally harvest
Haematococcus pluvialis biomass.
Fig. 6 shows the unit area light source yield of haematococcus pluvialis under different support column heights.
Detailed description of the invention
The present invention provides a kind of haematococcus pluvialis cultural methods, including in the thin layer with a thickness of 0.5mm-2.5mm
Haematococcus pluvialis described in fluid nutrient medium static culture.In some embodiments, the haematococcus pluvialis is haematococcus pluvialis
K-0084 algae strain, obtained from the Scandinavian Culture Collection of Algae of University Copenhagen Denmark
and Protozoa。
Since the thickness of culture medium in method of the invention is sufficiently small (0.5mm-2.5mm), do not need to be stirred
Etc. the operation that homogenizes, it still is able to guarantee that the haematococcus pluvialis cell in culture solution more can equably obtain illumination (in light
Line is into the effective distance transmitted under water).And by using culture medium thickness of the present invention (0.5mm-2.5mm), make
CO required for photosynthesis can be directly obtained from the liquid level of culture solution by gas exchanges by obtaining haematococcus pluvialis cell2Side by side
It puts and the O inhibited is generated to photosynthesis2, therefore do not need to carry out aeration to culture solution.
Frond harvest and drying are the important steps of algae culture, and energy consumption is high, low efficiency.However, according to the present invention
Method, using thin layer fluid nutrient medium static culture haematococcus pluvialis, (generally about 8-10 when algae grows to maturation
It), culture medium is generally also close to be completely consumed.At this point, dry cell weight has been reached compared with ratios, it is very beneficial for frond harvest, does
It is dry.It, can be according to haematococcus pluvialis inoculum concentration, light intensity, humidity within the scope of culture medium thickness of the invention (0.5mm-2.5mm)
The thickness further progress of culture medium is adjusted with factors such as temperature, so that the amount of culture medium grows into the maturity period just for algae
Without having excessive have more than needed.Further, since frond is constantly in Thin cell layer liquid, it is less susceptible to bacterium of mildewing.
Culture medium thickness used in method of the invention can be about 0.5mm, about 0.7mm, about 0.9mm,
Range between about 1mm, about 1.5mm, about 2mm, about 2.5mm or the arbitrary value.
In some embodiments, temperature used in the haematococcus pluvialis cultural method is 22-28 DEG C.In some realities
It applies in mode, light intensity used in the haematococcus pluvialis culture is 1-600umolm-2·s-1.In some embodiments,
Culture medium used in the haematococcus pluvialis culture is BG11 culture medium.In some embodiments, the haematococcus pluvialis
Ambient humidity range in culture is 80-99%.In some embodiments, haematococcus pluvialis in the haematococcus pluvialis culture
The density of initial inoculation is 0.1-0.3OD750, wherein OD750It is the absorbance measured under 750nm wavelength.0.1-0.3OD750It is corresponding
Initial inoculation biomass 0.16gL-1-0.54g·L-1。
Haematococcus pluvialis cultural method of the invention by the way of thin layer static culture, its essence is between immersion method and
A kind of novel haematococcus pluvialis cultural method between semidry method reduces toxigenic capacity, subtracts as described above, having energy saving
The advantage of few mould contamination risk.
On the other hand, the present invention also provides a kind of stacked thin plate haematococcus pluvialis culture apparatus comprising perpendicular
Histogram is laminated upwards and in multiple thin plate of preset space length arrangement, and the multiple thin plate is horizontally extending, each thin plate tool
There are the upper surface and opposite lower surface for placing haematococcus pluvialis culture solution, the periphery of each thin plate is equipped with from corresponding thin plate
The cofferdam that the upper surface for favouring corresponding thin plate stretched out upwardly extends, the cofferdam are higher by the upper surface of corresponding thin plate
1.5mm-4.5mm。
Fig. 1 to 3 shows stacked thin plate haematococcus pluvialis culture apparatus 100 according to the preferred embodiment of the invention.
It can be seen from the figure that stacked thin plate haematococcus pluvialis culture apparatus 100 is included in vertical direction upper layer laying up
The multiple thin plates 20 set and multiple support columns 40 between adjacent thin plate.Each thin plate 20 is horizontally extending, and
With the upper surface 22 and opposite lower surface 24 for placing haematococcus pluvialis culture solution.The periphery of each thin plate 20 be equipped with from
The cofferdam 26 that the upper surface 22 for favouring corresponding thin plate 20 that corresponding thin plate 20 stretches out upwardly extends.
Each thin plate 20 forms the culture plate of culture apparatus, that is, the upper surface 22 of each thin plate 20, the cofferdam 26 of thin plate 20
Inner surface limits the space 30 for containing haematococcus pluvialis culture solution.
The transparent plate made of glass material or organic material can be used in the thin plate 20 for forming culture apparatus.Relative to pipe
For shape, channel-shaped or can-like culture apparatus, the haematococcus pluvialis culture apparatus according to the present invention being made of plate adds
Work is simple to manufacture, is at low cost, and can accomplish that the culture area of single culture plate is larger, and thickness can also be relatively thin, thoroughly
Lightness can be higher.The length of the thin plate for example can be 10cm-10m, and width for example can be 5cm-1m.Some embodiment party
In formula, the specification of the thin plate is length 12cm × width 6cm.
Each thin plate 20 arranged stacked in the vertical direction, keeps structure integrated, effective culture area of unit space occupied
It maximizes.
Since the material of each thin plate 20 is the high material of transparency, in the Initial stage of culture of haematococcus pluvialis, due to each thin plate
Dark biomembrane has not yet been formed in haematococcus pluvialis on 20, therefore can reach through each 20 upper surface of layer thin plate from external light
Onto the culture liquid level of adjacent lower layer's thin plate, the culture of upper layer thin plate can also be equally reached from the lower surface of each layer thin plate 20
On liquid level, light efficiency is higher.Light source from the frustule above the upper surface direct irradiation of thin plate 20 and through thin plate 20 and
It is irradiated to the cell cultivated on the upper surface 22 of following thin plate 20, so light-receiving area is big, light utilization ratio is very high.
Using the haematococcus pluvialis culture apparatus of this structure, the water body used is few, so the culture of haematococcus pluvialis is not necessarily to
Energy consumption is saved in stirring.In addition, since water is few, when harvest, which can reduce process thus, reduces harvest cost.Thus, utilize this
The haematococcus pluvialis culture apparatus culture haematococcus pluvialis of structure is static gas wave refrigerator, is not necessarily to convey gas or offer in incubation
Stirring power, and illumination can be obtained well.
According to the present invention, the cofferdam 26 of each thin plate 20 can be integrally formed with thin plate 20, after can also independently forming
It is attached to thin plate 20.
When thin plate 20 is made of glass material, cofferdam 26 can be the circle adhesive tape for being adhered to 20 periphery of thin plate, excellent
Select cellophane adhesive tape.For the thin plate 20 of organic material, cofferdam 26 can be integrally formed with thin plate 20.
The material for forming thin plate 20 can be glass or the high plastics of light transmittance, such as GPPS, transparent ABS, AS (styrene third
Alkene nitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene) etc..Form the material of cofferdam 26
Material can be material identical with thin plate 20, can also use other transparent materials, such as adhesive tape, glass cement.
The upper surface 22 of cofferdam 26 and thin plate 20 is at the angle for being greater than or equal to 90 degree, in order to train on upper surface 22
Feeding haematococcus pluvialis is gone out with high pressure water/air-blowing to outside thin plate 20, to realize the harvest of haematococcus pluvialis；Preferably enclose
Weir portion 26 and the upper surface of thin plate 20 22 at the angle greater than 90 degree and form fillet between the two junction, prevent from being formed
Harvest dead angle or cleaning dead angle.Optionally, the cofferdam 26 be higher by the upper surface 22 of corresponding thin plate 20 distance be 1.5mm,
Range between 2.5mm, 3.5mm, 4.5mm or above-mentioned arbitrary value.For example, the distance can be 1.5mm-4.5mm.
Spacing (table on vertical range or two neighboring thin plate between two neighboring thin plate lower surface between each thin plate 20
Vertical range between face) it is required according to light source arrangement, the culture of haematococcus pluvialis and harvest to determine.For example, each thin plate 20 it
Between spacing be 4.5mm, 5.5mm, 6.5mm, 7.5mm, 8.5mm, 9.5mm, 10.5mm, 11.5mm, 12.5mm, 13.5mm,
Range between 14.5mm or 15.5mm or above-mentioned arbitrary value, such as 4.5-9.5mm or 10.5mm-15.5mm.
Shape, material, quantity, spacing and the arrangement of support column 40 can not limit, and support column 40 is only needed to have
Some strength, the culture solution being supported on the thin plate 20 and its upper surface 22 of stacked on top enough.Preferably, support column 40 uses
Transparent material, such as glass, acrylic, PC material.Support column 40 can have any suitable shape, including but not limited to round
Cylindricality, tubular, rectangular, strip etc..
In the illustrated embodiment, support column 40 is arranged in banks.Additionally optionally, it is provided on support column 40 for making light
The hole that can be penetrated.
For sheets of glass 20, support column 40 can use glass column, be also possible to non-glass column.Support column 40 can be with
It is all separated with above and below thin plate 20, the lower surface 24 of thin plate 20 above can also be adhered to.
For the thin plate 20 made of organic transparent material, support column 40 can be integrally formed with thin plate 20.
Stacked thin plate haematococcus pluvialis culture apparatus of the invention, which may further be provided, to be supplemented to each thin plate 20
The culture solution drop instillator 50 of culture solution, culture solution drop instillator 50 are schematically shown in Fig. 3, and optionally, can
Configuration measures the leve monitor (not shown) of the accurate liquid level of each thin plate 20.In addition light source 60 is shown in FIG. 3.Light source can
To be arranged in the stacked thin plate haematococcus pluvialis culture apparatus side of the present invention.
Additionally optionally, stacked thin plate haematococcus pluvialis culture apparatus of the invention can further include by stacked thin plate rain
The light-transmissive film cover that raw haematococcus culture apparatus is encapsulated provides for its internal stacked thin plate haematococcus pluvialis culture apparatus
Steady temperature, the atmosphere of air humidity.This can reduce the evaporation of moisture, create to the culture of haematococcus pluvialis most appropriate
Growth conditions so can no longer need to the culture solution that replenishes the supply in the cultivation cycle of haematococcus pluvialis.
Other than the stacked thin plate haematococcus pluvialis culture apparatus of above-mentioned example, each thin plate 20 can also be placed respectively
On the bracket with several cross bars, the gravity of each thin plate 20 and culture solution is dispersed by the bracket；Equally it can achieve
Technical effect identical with previous examples.
Present invention haematococcus pluvialis cultural method above-mentioned can be in stacked thin plate haematococcus pluvialis culture of the invention
It is carried out in device, allows to scale, cultivate haematococcus pluvialis at low cost.
In some embodiments of haematococcus pluvialis cultural method, multiple stacked thin plate haematococcus pluvialis culture dresses
Array can be arranged in the horizontal direction by setting.In some embodiments, wherein the side in array is disposed with light source.It is multiple
Culture apparatus is horizontally disposed at array, since cofferdam separates each thin plate, can make the pollution quilt being likely to occur in culture
It is limited in lesser range.
High density stacking culture is carried out to haematococcus pluvialis using culture apparatus of the invention, between having between every layer
Gap propagates to larger distance for light, therefore, light can and in the range of can be used to culture haematococcus pluvialis.Cause
This directly increases the yield of biomass of unit area light source, substantially increases the efficiency of light energy utilization.
Use the device of the invention culture haematococcus pluvialis, it is assumed that interlamellar spacing 4.9mm-11.9mm, culture solution with a thickness of
0.5mm-2.5mm, then 84-204 layers can be stacked under 1m height space.If it is raw to cultivate rain in the cofferdam of each layer of thin plate
Haematococcus, the then biomass that can be cultivated in 1m altitude range are considerable.It can reach cell in following examples of the present invention
Dry weight 416g/m3, net increment reaches 309g/m3, the net yield of flat light source area reach 29.4-30.9g/m2/ d, unit mass
The energy consumption of haematococcus pluvialis is only about 213-405kJ/g.
Embodiment
In order to further display the advantageous effects of cultural method and culture apparatus of the invention, following embodiment pair
" unit volume yield " " the net yield of unit area light source " " unit mass algae powder energy consumption " " unit mass algae powder water consumption " etc. because
Element is investigated.
Experimental group:
It (is also named as using the culture apparatus culture haematococcus pluvialis K-0084 algae strain of the invention for being arranged to array
CW316).The array top view is shown in Fig. 4 A.
Left side is that length is 1m, is highly the light source of 1m, light intensity 600umolm-2·s-1.Right side is several present invention trainings
Support the array of device composition.10 row's culture apparatus are arranged on the direction vertical with light source.Wherein, each thin plate (glass plate) rule
Lattice are 120mm × 60mm, and interlamellar spacing is 11.9mm, 6.8mm and 4.9mm respectively, is laminated to 1m in vertical direction.The height in cofferdam
Degree is about 1.5mm.Each thin plate is paved with haematococcus pluvialis culture solution.Each open ended algae solution amount of thin sheet surface is about
10ml.Environment temperature controls 25 degree or so, relative humidity 80%-99%.Initial inoculation OD on each thin plate750=0.2, it is right
Answer inoculum concentration 0.35gL-1.Culture carries out in the environment of completely obscured natural light, excludes natural light interference.
Detected through light-intensity test instrument, from apart from lamp source it is farthest the 10th drain into culture medium in the 1st nearest row's culture apparatus
The light intensity on surface is shown in Fig. 5.From fig. 5, it can be seen that existing between layers a large amount of due to there is support column between the thin plate of stacking
Tiny spacing, light can be continuously passed on by the gap of interlayer toward distal side, and remoter local light intensity is weaker.Fig. 5 is each
It is successively from left to right the rain that the 10th row, the light intensity value of the 1st row's media surface of the 9th row ... and each row finally harvest in width figure
Raw haematococcus biomass.
And it is observed that the sheets of glass of the 1st row since close to light source, upgrowth situation is best, is grown further away from light source lamp plate
Situation is poorer, and the growing state of the 10th row is worst.Wait be harvested after cultivating about 6 days, it is total to count each row's haematococcus pluvialis
Biomass, growing state are as shown in Figure 5.It can therefrom see, although the haematococcus pluvialis growing state of the 10th row is poor,
Light source can still be obtained and grown.The number of rows arranged on the direction vertical with light source can be adjusted according to the intensity of light source with
Obtain suitable haematococcus pluvialis growing.
Control group:
It is carried out in the environment of completely obscured natural light using classic flat-plate vessel type reactor (goldfish jar formula reactor)
Haematococcus pluvialis culture.As shown in the top view of Fig. 4 B, the light source of 1 ㎡ irradiates a side of traditional plate container type reactor
Face, plate container side are 1 ㎡, container width 5cm.Classic flat-plate vessel type reactor needs to stir and special CO2It is high
Press tank gas supply.
Light passes through the light intensity measurement almost 0 after 5cm algae solution, and light can not be propagated further, and lead to the light source of 1 ㎡ only
It can be for the haematococcus pluvialis growing in the irradiation plate container of one row.The volume of plate container is 1 ㎡ * 0.05m=50L.
As a result compare:
It is anti-using stacked thin plate haematococcus pluvialis culture apparatus of the invention and traditional plate container type as described above
The result of device culture haematococcus pluvialis is answered to be summarized as follows table 1 (using 1 ㎡ light source as references object):
Table 1
| The present invention | Flat plate photobioreactor | |
| Unit volume yield | 1.01g/L | 0.73g/L |
| The net yield of unit area light source | 29.4-30.9g/m2/d | 3.65-7.25g/m2/d |
| Unit mass algae powder water consumption | 0.99L/g | 1.37L/g |
| Unit mass algae powder energy consumption | 213-405kJ/g | 1717kJ/g |
Fig. 6 is shown in when interlamellar spacing is 11.9mm, 6.8mm, 4.9mm and is produced using the unit area light source of the device of the invention
Rate is respectively 10.83g/m2/ d, 15.44g/m2/ d, 19.46g/m2/d.It can be seen that using culture apparatus of the invention, above-mentioned interlayer
Away under, the yield of unit area is higher than traditional plate container.But if spacing is too small, the algae solution glass easy to stick for connecting one layer
Glass thin plate, influences the growth of haematococcus pluvialis, and suitable spacing should be not less than 4.5mm.
As it can be seen that the method and apparatus that haematococcus pluvialis cultural method of the invention and device are significantly better than the prior art.This
Outside, cultural method and device of the present invention are to cultivate in a static manner, during which without for high pressure CO2Realize air-flotation type stirring,
Without equipment such as circulating pump, agitating paddles, energy consumption is further reduced.In addition, the present invention uses Thin cell layer, the rain life of harvest is red
Ball algae has been in half wet condition, further reduces the energy consumption of concentrate drying.
Claims (23)
1. a kind of stacked thin plate haematococcus pluvialis culture apparatus, characterized in that the stacked thin plate haematococcus pluvialis culture
Device includes multiple thin plates of the arranged stacked on vertical direction, and the multiple thin plate is supported so that between adjacent thin plate
Every a preset distance, each thin plate is horizontally extending, has opposite upper and lower surfaces, and be provided peripherally with from phase
The cofferdam for answering the upper surface of thin plate to upwardly extend, the upper surface of each thin plate and the inner surface of cofferdam form raw for accommodating rain
The space of haematococcus culture solution, wherein the cofferdam is higher by the upper surface 1.5mm-4.5mm of corresponding thin plate, wherein each thin plate it
Between spacing it is identical or different.
2. the stacked thin plate haematococcus pluvialis culture apparatus of claim 1, characterized in that between the adjacent thin plate of up and down direction
Multiple support columns are set, and the support column is used to support thin plate above.
3. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the cofferdam with it is corresponding thin
Plate is integrally formed, or corresponding thin plate is attached to after independently forming.
4. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the support column with it is corresponding thin
Plate is integrally formed, or the lower surface of corresponding thin plate is attached to after independently forming.
5. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the cofferdam is relative to phase
Answer the upper surface of thin plate at the inclination angle for being greater than or equal to 90 degree.
6. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the cofferdam by with thin plate
Identical or different material is made and/or the support column is made of the material identical or different with thin plate.
7. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the support column is that have circle
The form of shape, the column of rectangle or square cross section or pipe.
8. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the thin plate by light transmission glass
Glass or the high plastics of light transmittance are made.
9. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the thin plate is by materials described below
One of be made: glass, GPPS, transparent ABS, AS (styrene-acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC are (poly-
Carbonic ether), PS (polystyrene).
10. the stacked thin plate haematococcus pluvialis culture apparatus of claim 1, characterized in that the thin plate is by plate glass system
At, and the cofferdam is attached to the adhesive tape of thin plate.
11. the stacked thin plate haematococcus pluvialis culture apparatus of claim 10, wherein the adhesive tape is cellophane adhesive tape.
12. the stacked thin plate haematococcus pluvialis culture apparatus of claim 1, characterized in that the thin plate is by organic material system
At, and the cofferdam and thin plate are integrally formed.
13. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the spacing between adjacent sheet
For 4.5mm-9.5mm or 10.5mm-15.5mm.
14. the stacked thin plate haematococcus pluvialis culture apparatus of claims 1 or 2, characterized in that the specification of the thin plate is length
Spend 12cm × width 6cm.
15. the stacked thin plate haematococcus pluvialis culture apparatus of claim 2, characterized in that form luminous energy on the support column
The hole enough penetrated.
16. a kind of haematococcus pluvialis cultural method, including quiet in the thin layer fluid nutrient medium with a thickness of 0.5mm-2.5mm
Haematococcus pluvialis described in state culture, wherein haematococcus pluvialis culture is raw in the stacked thin plate rain of any one of claim 1-15
It is carried out in haematococcus culture apparatus.
17. the method for claim 16, wherein the haematococcus pluvialis is haematococcus pluvialis K-0084 algae strain.
18. the method for claim 16 or 17, wherein the culture carries out at 20-30 DEG C.
19. the method for claim 16 or 17, wherein the culture is in 1-600umolm-2·s-1Light intensity under carry out.
20. the method for claim 16 or 17, wherein the culture medium is BG11 culture medium.
21. the method for claim 16 or 17, wherein the culture carries out under the humidity of 80-99%.
22. the method for claim 16 or 17, wherein the density of the initial inoculation of haematococcus pluvialis is 0.1-0.3OD750。
23. the method for claim 16, plurality of stacked thin plate haematococcus pluvialis culture apparatus is arranged in the horizontal direction
At array, wherein the side in array is disposed with light source.
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| CN110423675B (en) * | 2019-09-11 | 2024-08-23 | 宁波大学 | Large-scale haematococcus pluvialis cultivation device manufactured by using ecological niche principle |
| CN113214962A (en) * | 2021-05-10 | 2021-08-06 | 东台市赐百年生物工程有限公司 | Multilayer microalgae breeding device |
| CN119040094B (en) * | 2024-09-05 | 2025-01-21 | 云南爱尔发生物技术股份有限公司 | A Haematococcus algae cultivation device based on the niche principle |
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