CN106924808A - A kind of fibroin tissue renovation material containing the fruit of summer cypress - Google Patents
A kind of fibroin tissue renovation material containing the fruit of summer cypress Download PDFInfo
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- CN106924808A CN106924808A CN201710206195.9A CN201710206195A CN106924808A CN 106924808 A CN106924808 A CN 106924808A CN 201710206195 A CN201710206195 A CN 201710206195A CN 106924808 A CN106924808 A CN 106924808A
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- Prior art keywords
- fibroin
- fruit
- summer cypress
- solution
- parts
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- 108010022355 Fibroins Proteins 0.000 title claims abstract description 85
- 241001645380 Bassia scoparia Species 0.000 title claims abstract description 44
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 44
- 239000000463 material Substances 0.000 title claims abstract description 34
- 238000009418 renovation Methods 0.000 title claims abstract description 20
- 239000002121 nanofiber Substances 0.000 claims abstract description 27
- 239000000284 extract Substances 0.000 claims abstract description 24
- BLXXJMDCKKHMKV-UHFFFAOYSA-N Nabumetone Chemical compound C1=C(CCC(C)=O)C=CC2=CC(OC)=CC=C21 BLXXJMDCKKHMKV-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229960004270 nabumetone Drugs 0.000 claims abstract description 19
- 239000003094 microcapsule Substances 0.000 claims abstract description 15
- 239000000126 substance Substances 0.000 claims abstract description 15
- 239000011148 porous material Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 23
- 239000000706 filtrate Substances 0.000 claims description 16
- 238000009987 spinning Methods 0.000 claims description 14
- 239000012141 concentrate Substances 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 238000010041 electrostatic spinning Methods 0.000 claims description 8
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 8
- 238000003760 magnetic stirring Methods 0.000 claims description 8
- 238000000967 suction filtration Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 6
- 241000255789 Bombyx mori Species 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000000502 dialysis Methods 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000004945 emulsification Methods 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 239000000839 emulsion Substances 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 4
- 239000003517 fume Substances 0.000 claims description 4
- 239000000499 gel Substances 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 4
- 238000004804 winding Methods 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims 1
- 210000000056 organ Anatomy 0.000 abstract description 8
- 230000017423 tissue regeneration Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 230000006870 function Effects 0.000 abstract description 5
- 239000012620 biological material Substances 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 230000008827 biological function Effects 0.000 abstract description 2
- 230000008439 repair process Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 60
- 210000001519 tissue Anatomy 0.000 description 20
- 238000001035 drying Methods 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 230000001741 anti-phlogistic effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- -1 amino, carboxyl Chemical group 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000009881 electrostatic interaction Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
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- A61L2300/622—Microcapsules
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- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
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Abstract
A kind of fibroin tissue renovation material containing the fruit of summer cypress, is the membranoid substance comprising fibroin, and internal average cell density is 1 3000/mm2, average pore size is 1 500 μm, described raw material of the membranoid substance comprising following parts by weight：70 100 parts of fibroin solutions, 69 parts of fibroin nanofiber solution, 79 parts of fruit of summer cypress extract solution, 23 parts of Nabumetone slow-release microcapsule.The present invention relates to biomaterial and fibroin field.Big tissue and the biomaterial of complex organ must can just make to organize greatly with the ability for promoting vascularization and complex organ has biological function that reparation is damaged.But it is generally in the prior art simple fibroin albumen repair materials, is extremely difficult to preferable rush vascularization and tissue repair function, and the obtained tissue renovation material of the present invention has the effect of more preferable tissue repair.
Description
Technical field
The present invention relates to biomaterial and fibroin field, more particularly to a kind of fibroin tissue containing the fruit of summer cypress is repaiied
Multiple material.
Background technology
Tissue engineering technique is a new tool for being used to treat tissue and organ defect, by biological support, cell, growth
The elements such as the factor are constituted.Wherein, the physical support that biological support is formed as cell growth, propagation and new organization, in tissue
Played an important role in engineering.Organizational project is intended to the 26S Proteasome Structure and Function integrality of restoration and reconstruction damaged tissues or organ, and shows
The present can't solve the reparation of complicated tissue organ.Big tissue, complex organ can be by small points unlike simple lamellar tissue
Son diffusion obtains nutriment and oxygen, and they need that by huge vascular system nutriment and oxygen could be obtained.Repair
The big tissue of multiple damage and the biomaterial of complex organ must can just make big tissue and complexity with the ability for promoting vascularization
Organ has biological function.
Fibroin albumen has excellent biocompatibility, degradability, improvability and mechanical stretching performance etc..Fibroin egg
Contain hydroxyl, amino, carboxyl isoreactivity group in white, hydrogen bond, electrostatic interaction, chelating can be passed through with other high molecular polymers
Effect etc. is combined, the superior compound bio timbering material of forming properties.But it is generally in the prior art simple fibroin albumen material
Material, is extremely difficult to preferable rush vascularization and tissue repair function.
The content of the invention
For problems of the prior art, the invention provides a kind of fibroin tissue repair material containing the fruit of summer cypress
Material, compared with the prior art in simple fibroin material there is more preferable tissue repair function.
In order to solve the above problems, the present invention uses following technical scheme：
A kind of fibroin tissue renovation material containing the fruit of summer cypress, is the membranoid substance comprising fibroin, internal average hole
Density is 1-3000/mm2, average pore size is 1-500 μm, described raw material of the membranoid substance comprising following parts by weight：Silk
Protein solution 70-100 parts, 6-9 parts of fibroin nanofiber solution, 7-9 parts of fruit of summer cypress extract solution, Nabumetone slow-release microcapsule
2-3 parts.
Preferably, described fruit of summer cypress extract solution is prepared using following methods：Fruit of summer cypress 25g is weighed, meal is ground into, plus
Enter the water of 10 times of its weight, decocted under the conditions of 90-100 DEG C and extract 1-1.5h, suction filtration obtains filtrate 1；Again to 6 times of addition in filter residue
The distilled water of filter residue weight, decocts under the conditions of 70-80 DEG C and extracts 1-1.5h, and suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously,
Temperature be 40-50 DEG C, vacuum be 0.05-0.08Pa under the conditions of be concentrated under reduced pressure into 4 times of fruit of summer cypress weight, filtering obtains final product ground
Skin extract solution.
Preferably, described Nabumetone slow-release microcapsule is the water that the Nabumetone of 2-3 mass parts is dissolved in 70-80 mass parts
In, and the hydrophilic emulsifier of 1-3 mass parts is added, and emulsion reaction is carried out in the presence of magnetic stirring apparatus and is obtained, it is described
The stir speed (S.S.) of magnetic stirring apparatus is 1000-3000rpm, and emulsification times are 30-40min.
Preferably, described fibroin nanofiber solution is prepared using following methods：Fibroin solutions are taken to be placed in
The half of original volume is concentrated into 60 DEG C of insulating boxs, then is transferred in fume hood and is concentrated into mass fraction for 20-30% obtains concentrate,
It is 0.5% concentrate to be put into concentrate is diluted with water into mass fraction after 4 DEG C of refrigerator storage 2d, is put after full and uniform stirring
Enter and be incubated in insulating box to the fibroin nanofiber solution for forming translucent gels shape.
Preferably, the length of fibroin nanofiber is 1 μm in described fibroin nanofiber solution, a diameter of
10-20nm。
Preferably, described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, is added
4.24g sodium carbonate and 5g silkworm raw silks, boil 30min, after being cleaned up with deionized water, drying, with the LiBr of 9.3mol/L
Solution dissolves；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated
Twice, fibroin solutions are obtained.
Preferably, described fibroin solutions are silk fibroin solution.
Preferably, described membranoid substance is prepared using following methods：By fibroin solutions and fibroin nanofiber
Solution mixes, and adds the well mixed prepared spinning solution of fruit of summer cypress extract solution, Nabumetone slow-release microcapsule, spinning solution to use electrostatic
Spinning technique is obtained three-D non-woven net-structured film.
It is further preferred that described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed
100-300rpm, spinning solution flow 2-5ml/h, 50-150 DEG C of heating-up temperature.
Preferably, fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-
500um, film thickness is 50nm-20mm.
The beneficial effects of the present invention are：
1) tissue renovation material of the invention, relative to simple fibroin material, is the soft tissue repair material of low modulus
Material, with preferably rush vascularization and tissue repair function.
2) three-dimensional porous material for organizational project must have porosity higher, with interconnected three-dimensional knot
Structure, in order to the propagation of cell, migration, for the growth of cell provides enough nutriments.After adding nanofiber, improve
There are nanofibrous structures on the pore-creating character support hole wall of support, the specific surface area of support is considerably increased.
3) medicinal herb componentses fruit of summer cypress extract solution, and Nabumetone slow-release microcapsule are added, the group of material is further increased
Knit the ability of reparation.
4) fruit of summer cypress has clearing heat and promoting diuresis, dispelling wind and arresting itching, and effect that anti-inflammatory relieves the pain can be used for various allergic skins and ask
Topic.
Specific embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of fibroin tissue renovation material containing the fruit of summer cypress, is the membranoid substance comprising fibroin, internal average hole
Density is 1-3000/mm2, average pore size is 1-500 μm, described raw material of the membranoid substance comprising following parts by weight：Silk
70 parts of protein solution, 6 parts of fibroin nanofiber solution, 7 parts of fruit of summer cypress extract solution, 2 parts of Nabumetone slow-release microcapsule.
Described fruit of summer cypress extract solution is prepared using following methods：Fruit of summer cypress 25g is weighed, meal is ground into, adds its heavy
The water of 10 times of amount, decocts under the conditions of 90-100 DEG C and extracts 1h, and suction filtration obtains filtrate 1；Again to 6 times of filter residue weight of addition in filter residue
Distilled water, decocts under the conditions of 70-80 DEG C and extracts 1h, and suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, it is 40-50 in temperature
DEG C, vacuum be to be concentrated under reduced pressure into 4 times of fruit of summer cypress weight under the conditions of 0.05-0.08Pa, filtering obtains final product fruit of summer cypress extract solution.
Described Nabumetone slow-release microcapsule is that the Nabumetone of 2 mass parts is dissolved in the water of 70 mass parts, and adds 1 matter
The hydrophilic emulsifier of part is measured, emulsion reaction is carried out in the presence of magnetic stirring apparatus and is obtained, described magnetic stirring apparatus is stirred
Speed is mixed for 1000-3000rpm, emulsification times are 30-40min.
Described fibroin nanofiber solution is prepared using following methods：Take fibroin solutions and be placed in 60 DEG C of constant temperature
The half of original volume is concentrated into case, then be transferred in fume hood be concentrated into mass fraction for 20% concentrate, concentrate is put
Enter that concentrate is diluted with water into mass fraction after 4 DEG C of refrigerator storage 2d is 0.5%, be put into insulating box after full and uniform stirring
It is incubated to the fibroin nanofiber solution for forming translucent gels shape.
The length of fibroin nanofiber is 1 μm, a diameter of 10- in described fibroin nanofiber solution
20nm。
Described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4.24g carbon is added
Sour sodium and 5g silkworm raw silks, boil 30min, and after being cleaned up with deionized water, drying, the LiBr solution with 9.3mol/L is molten
Solution；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice,
Obtain fibroin solutions.
Described fibroin solutions are silk fibroin solution.
Described membranoid substance is prepared using following methods：Fibroin solutions are mixed with fibroin nanofiber solution
Close, and add the well mixed prepared spinning solution of fruit of summer cypress extract solution, Nabumetone slow-release microcapsule, spinning solution to use electrostatic spinning work
Skill is obtained three-D non-woven net-structured film.
Described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin
Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film
Thickness is 50nm-20mm.
Embodiment 2
A kind of fibroin tissue renovation material containing the fruit of summer cypress, is the membranoid substance comprising fibroin, internal average hole
Density is 1-3000/mm2, average pore size is 1-500 μm, described raw material of the membranoid substance comprising following parts by weight：Silk
100 parts of protein solution, 9 parts of fibroin nanofiber solution, 9 parts of fruit of summer cypress extract solution, 3 parts of Nabumetone slow-release microcapsule.
Described fruit of summer cypress extract solution is prepared using following methods：Fruit of summer cypress 25g is weighed, meal is ground into, adds its heavy
The water of 10 times of amount, decocts under the conditions of 90-100 DEG C and extracts 1.5h, and suction filtration obtains filtrate 1；Again to 6 times of filter residue weight of addition in filter residue
Distilled water, decocted under the conditions of 70-80 DEG C and extract 1.5h, suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, be in temperature
40-50 DEG C, vacuum be to be concentrated under reduced pressure into 4 times of fruit of summer cypress weight under the conditions of 0.05-0.08Pa, filtering obtains final product fruit of summer cypress extraction
Liquid.
Described Nabumetone slow-release microcapsule is that the Nabumetone of 3 mass parts is dissolved in the water of 80 mass parts, and adds 3 matter
The hydrophilic emulsifier of part is measured, emulsion reaction is carried out in the presence of magnetic stirring apparatus and is obtained, described magnetic stirring apparatus is stirred
Speed is mixed for 1000-3000rpm, emulsification times are 30-40min.
Described fibroin nanofiber solution is prepared using following methods：Take fibroin solutions and be placed in 60 DEG C of constant temperature
The half of original volume is concentrated into case, then be transferred in fume hood be concentrated into mass fraction for 30% concentrate, concentrate is put
Enter that concentrate is diluted with water into mass fraction after 4 DEG C of refrigerator storage 2d is 0.5%, be put into insulating box after full and uniform stirring
It is incubated to the fibroin nanofiber solution for forming translucent gels shape.
The length of fibroin nanofiber is 1 μm, a diameter of 10- in described fibroin nanofiber solution
20nm。
Described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4.24g carbon is added
Sour sodium and 5g silkworm raw silks, boil 30min, and after being cleaned up with deionized water, drying, the LiBr solution with 9.3mol/L is molten
Solution；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice,
Obtain fibroin solutions.
Described fibroin solutions are silk fibroin solution.
Described membranoid substance is prepared using following methods：Fibroin solutions are mixed with fibroin nanofiber solution
Close, and add the well mixed prepared spinning solution of fruit of summer cypress extract solution, Nabumetone slow-release microcapsule, spinning solution to use electrostatic spinning work
Skill is obtained three-D non-woven net-structured film.
Described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin
Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film
Thickness is 50nm-20mm.
By tissue renovation material (S), tissue renovation material (SS) obtained in embodiment 2 obtained in embodiment 1, and by pure silkworm
The performance of the tissue renovation material (SSS) that silk fibroin albumen is made is contrasted, using with identical degree of tissue damage
Small white mouse is tested, and material S, SS and SSS are spread on the injury site of small white mouse respectively, is changed twice daily, is continuously used
10 days, using observation point system, small white mouse wounded tissue recovery extent is given a mark by 1-5 points respectively, every using effect
Optimal is 5 points, and the experimental data for drawing is shown in Table 1.
The resultant effect of table 1 is investigated
| Material | S | SS | SSS |
| Tissue repair degree | 4.6 | 4.7 | 2.7 |
| Antiphlogistic effects | 4.8 | 4.4 | 2.6 |
By experimental result it can be seen that：To addition fibroin nanofiber, the fruit of summer cypress, Nabumetone in silk fibroin
Slow-release microcapsule, can effectively lift tissue repairing ability and the antiphlogistic effects of fibroin albumen.
Claims (9)
1. a kind of fibroin tissue renovation material containing the fruit of summer cypress, is the membranoid substance comprising fibroin, internal average Kong Mi
It is 1-3000/mm to spend2, average pore size is 1-500 μm, it is characterised in that described membranoid substance includes following parts by weight
Raw material：Fibroin solutions 70-100 parts, 6-9 parts of fibroin nanofiber solution, 7-9 parts of fruit of summer cypress extract solution, Nabumetone
Slow-release microcapsule 2-3 parts；
Described fruit of summer cypress extract solution is prepared using following methods：Fruit of summer cypress 25g is weighed, meal is ground into, its weight 10 is added
Water again, decocts under the conditions of 90-100 DEG C and extracts 1-1.5h, and suction filtration obtains filtrate 1；Again to 6 times of filter residue weight of addition in filter residue
Distilled water, decocts under the conditions of 70-80 DEG C and extracts 1-1.5h, and suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, be in temperature
40-50 DEG C, vacuum be to be concentrated under reduced pressure into 4 times of fruit of summer cypress weight under the conditions of 0.05-0.08Pa, filtering obtains final product fruit of summer cypress extraction
Liquid.
2. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 1, it is characterised in that described
Nabumetone slow-release microcapsule is that the Nabumetone of 2-3 mass parts is dissolved in the water of 70-80 mass parts, and adds 1-3 mass parts
Hydrophilic emulsifier, emulsion reaction is carried out in the presence of magnetic stirring apparatus and is obtained, the stir speed (S.S.) of described magnetic stirring apparatus
It is 1000-3000rpm, emulsification times are 30-40min.
3. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 1, it is characterised in that described
Fibroin nanofiber solution is prepared using following methods：Take fibroin solutions and be placed in 60 DEG C of insulating boxs and be concentrated into substance
Long-pending half, then be transferred in fume hood and be concentrated into mass fraction for 20-30% obtains concentrate, concentrate is put into 4 DEG C of refrigerators storages
It is 0.5% to deposit and concentrate is diluted with water into mass fraction after 2d, is put into after full and uniform stirring in insulating box and is incubated to being formed
The fibroin nanofiber solution of translucent gels shape.
4. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 3, it is characterised in that：Described
The length of fibroin nanofiber is 1 μm, a diameter of 10-20nm in fibroin nanofiber solution.
5. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 1, it is characterised in that described
Fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4.24g sodium carbonate and the life of 5g silkworms is added
Silk, boils 30min, after being cleaned up with deionized water, dry, with the LiBr solution dissolving of 9.3mol/L；Until completely dissolved
Solution is loaded into bag filter, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, and obtains fibroin solutions.
6. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 1, it is characterised in that：Described
Fibroin solutions are silk fibroin solution.
7. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 1, it is characterised in that described
Membranoid substance is prepared using following methods：Fibroin solutions are mixed with fibroin nanofiber solution, and adds the fruit of summer cypress
The well mixed prepared spinning solution of extract solution, Nabumetone slow-release microcapsule, spinning solution is obtained three-D non-woven using electrostatic spinning process
Net-structured film.
8. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 7, it is characterised in that described
Electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spinning solution flow 2-5ml/
H, 50-150 DEG C of heating-up temperature.
9. a kind of fibroin tissue renovation material containing the fruit of summer cypress according to claim 7, it is characterised in that：Described
Fibre diameter is 50nm-20um in three-D non-woven net-structured film, and interfiber pore is l-500um, and film thickness is 50nm-
20mm。
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| CN1397354A (en) * | 2002-08-14 | 2003-02-19 | 苏州大学 | Porous material for scaffold of tissue engineering and its preparing process |
| EP1613796A2 (en) * | 2003-04-10 | 2006-01-11 | Tufts University | Concentrated aqueous silk fibroin solution and use thereof |
| CN101036802A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and the preparation method |
| CN102512710A (en) * | 2011-12-28 | 2012-06-27 | 苏州大学 | Preparation method of silk fibroin porous three-dimensional material |
| CN103341209A (en) * | 2013-07-08 | 2013-10-09 | 苏州大学 | Silk fibroin nanofiber membrane and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397354A (en) * | 2002-08-14 | 2003-02-19 | 苏州大学 | Porous material for scaffold of tissue engineering and its preparing process |
| EP1613796A2 (en) * | 2003-04-10 | 2006-01-11 | Tufts University | Concentrated aqueous silk fibroin solution and use thereof |
| CN101036802A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and the preparation method |
| CN102512710A (en) * | 2011-12-28 | 2012-06-27 | 苏州大学 | Preparation method of silk fibroin porous three-dimensional material |
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