CN107029289A - A kind of spider silk fibroin tissue renovation material - Google Patents
A kind of spider silk fibroin tissue renovation material Download PDFInfo
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- CN107029289A CN107029289A CN201710206322.5A CN201710206322A CN107029289A CN 107029289 A CN107029289 A CN 107029289A CN 201710206322 A CN201710206322 A CN 201710206322A CN 107029289 A CN107029289 A CN 107029289A
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- Prior art keywords
- spider silk
- silk fibroin
- solution
- parts
- spider
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- 108010022355 Fibroins Proteins 0.000 title claims abstract description 84
- 229920001872 Spider silk Polymers 0.000 title claims abstract description 62
- 239000000463 material Substances 0.000 title claims abstract description 33
- 238000009418 renovation Methods 0.000 title claims abstract description 20
- 239000002121 nanofiber Substances 0.000 claims abstract description 26
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229960005489 paracetamol Drugs 0.000 claims abstract description 18
- 239000003094 microcapsule Substances 0.000 claims abstract description 15
- 239000000126 substance Substances 0.000 claims abstract description 14
- 239000000341 volatile oil Substances 0.000 claims abstract description 11
- 239000011148 porous material Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 63
- 238000000034 method Methods 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 238000009987 spinning Methods 0.000 claims description 14
- 239000012141 concentrate Substances 0.000 claims description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000010041 electrostatic spinning Methods 0.000 claims description 7
- 238000003760 magnetic stirring Methods 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 239000012460 protein solution Substances 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000000502 dialysis Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000004945 emulsification Methods 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 239000000839 emulsion Substances 0.000 claims description 4
- 239000003517 fume Substances 0.000 claims description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 4
- 238000004804 winding Methods 0.000 claims description 4
- 101710089165 Protein white Proteins 0.000 claims 3
- 241000239290 Araneae Species 0.000 claims 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 210000000056 organ Anatomy 0.000 abstract description 8
- 230000017423 tissue regeneration Effects 0.000 abstract description 8
- 230000006870 function Effects 0.000 abstract description 5
- 239000012620 biological material Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008827 biological function Effects 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 230000008439 repair process Effects 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 20
- 239000000499 gel Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000001741 anti-phlogistic effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- -1 amino, carboxyl Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001523 electrospinning Methods 0.000 description 1
- 230000009881 electrostatic interaction Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
- A61L2300/622—Microcapsules
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- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
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Abstract
A kind of spider silk fibroin tissue renovation material, for the membranoid substance comprising spider silk fibroin, internal average cell density is 1 2000/mm2, average pore size is 1 300 μm, it is characterised in that described membranoid substance includes the raw material of following parts by weight：80 90 parts of spidroin solution, 46 parts of spider silk fibroin nanofiber solution, 45 parts of paracetamol slow-release microcapsule, 12 parts of essential oil.The present invention relates to biomaterial and spider silk fibroin field.The biomaterial of the big tissue and complex organ of repairing damage must have the ability for promoting vascularization big tissue and complex organ is had biological function.But it is generally in the prior art simple fibroin albumen repair materials, is extremely difficult to preferable rush vascularization and tissue repair function, and tissue renovation material produced by the present invention has the effect of more preferable tissue repair.
Description
Technical field
The present invention relates to biomaterial and spider silk fibroin field, more particularly to a kind of spider silk fibroin tissue repair material
Material.
Background technology
Tissue engineering technique is a new tool for being used to treat tissue and organ defect, by biological support, cell, growth
The elements such as the factor are constituted.Wherein, the physical support that biological support is formed as cell growth, propagation and new organization, in tissue
Played an important role in engineering.Organizational project is intended to the 26S Proteasome Structure and Function integrality of restoration and reconstruction damaged tissues or organ, and shows
The present can't solve the reparation of complicated tissue organ.Big tissue, complex organ can pass through small point unlike simple lamellar tissue
Son diffusion obtains nutriment and oxygen, and they need that by huge vascular system nutriment and oxygen could be obtained.Repair
The big tissue and the biomaterial of complex organ damaged again must have the ability for promoting vascularization just to make big tissue and complicated
Organ has biological function.
Fibroin albumen has excellent biocompatibility, degradability, improvability and mechanical stretching performance etc..Fibroin egg
Contain hydroxyl, amino, carboxyl isoreactivity group in white, hydrogen bond, electrostatic interaction, chelating can be passed through with other high molecular polymers
Effect etc. is combined, the superior compound bio timbering material of forming properties.But it is generally simple fibroin albumen material in the prior art
Material, is extremely difficult to preferable rush vascularization and tissue repair function.
The content of the invention
For problems of the prior art, the invention provides a kind of spider silk fibroin tissue renovation material, relatively
Simple fibroin material has more preferable tissue repair function in the prior art.
In order to solve the above problems, the present invention uses following technical scheme：
A kind of spider silk fibroin tissue renovation material, for the membranoid substance comprising spider silk fibroin, internal average cell density is
1-2000/mm2, average pore size is 1-300 μm, and described membranoid substance includes the raw material of following parts by weight：Spider silk fibroin
80-90 parts of solution, 4-6 parts of spider silk fibroin nanofiber solution, 4-5 parts of paracetamol slow-release microcapsule, essential oil 1-2
Part.
It is preferred that, described paracetamol slow-release microcapsule is that the paracetamol of 2-3 mass parts is dissolved in into 70-
In the water of 80 mass parts, and the hydrophilic emulsifier of 1-3 mass parts is added, emulsion reaction is carried out in the presence of magnetic stirring apparatus
It is made, the stir speed (S.S.) of described magnetic stirring apparatus is 1000-3000rpm, and emulsification times are 30-40min.
It is preferred that, described spider silk fibroin nanofiber solution is prepared using following methods：Take spidroin solution
It is placed in 60 DEG C of insulating boxs and is concentrated into the half of original volume, then is transferred in fume hood and is concentrated into mass fraction and obtains dense for 20-30%
Contracting liquid, it is 0.5% concentrate to be put into after 4 DEG C of refrigerator storage 2d concentrate is diluted with water into mass fraction, full and uniform to stir
It is put into insulating box and is incubated to the spider silk fibroin nanofiber solution for forming translucent gels shape after mixing.
It is preferred that, the length of spider silk fibroin nanofiber is 1 μm in described spider silk fibroin nanofiber solution, directly
Footpath is 10-20nm.
It is preferred that, described spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, plus
Enter 4.24g sodium carbonate and 5g spider silks, boil 30min, after being cleaned up, drying with deionized water, with 9.3mol/L LiBr
Solution dissolves；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated
Twice, spidroin solution is obtained.
It is preferred that, described spidroin solution is spider silk silk fibroin protein solution.
It is preferred that, described membranoid substance is prepared using following methods：By spidroin solution and spider silk fibroin nanometer
Fiber solution is mixed, and adds the well mixed obtained spinning solution of paracetamol slow-release microcapsule, essential oil, and spinning solution is using quiet
Three-D non-woven net-structured film is made in electrospinning process.
It is further preferred that described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed
100-300rpm, spinning solution flow 2-5ml/h, 50-150 DEG C of heating-up temperature.
It is preferred that, fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-
500um, film thickness is 50nm-20mm.
The beneficial effects of the present invention are：
1) tissue renovation material of the invention is relative to silk fibroin material, for the soft tissue repair material of low modulus
Material, vascularization and tissue repair function are promoted with more preferable.
2) three-dimensional porous material for organizational project must have higher porosity, with interconnected three-dimensional knot
Structure, propagation, migration in order to cell, enough nutriments are provided for the growth of cell.Add after nanofiber, improve
There are nanofibrous structures on the pore-creating character support hole wall of support, the specific surface area of support is considerably increased.
3) addition paracetamol slow-release microcapsule, essential oil, further increase the ability of materials microstructure reparation.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of spider silk fibroin tissue renovation material, for the membranoid substance comprising spider silk fibroin, internal average cell density is
1-2000/mm2, average pore size is 1-300 μm, it is characterised in that described membranoid substance includes the raw material of following parts by weight：
80 parts of spidroin solution, 4 parts of spider silk fibroin nanofiber solution, 4 parts of paracetamol slow-release microcapsule, essential oil 1
Part.
Described paracetamol slow-release microcapsule is the water that the paracetamol of 2 mass parts is dissolved in 70 mass parts
In, and the hydrophilic emulsifier of 1 mass parts is added, and emulsion reaction is carried out in the presence of magnetic stirring apparatus and is made, described magnetic
The stir speed (S.S.) of power agitator is 1000-3000rpm, and emulsification times are 30-40min.
Described spider silk fibroin nanofiber solution is prepared using following methods：Spidroin solution is taken to be placed in 60 DEG C
The half of original volume is concentrated into insulating box, then be transferred in fume hood be concentrated into mass fraction for 20% concentrate, will concentrate
It is 0.5% that liquid, which is put into after 4 DEG C of refrigerator storage 2d concentrate being diluted with water mass fraction, and constant temperature is put into after full and uniform stirring
It is incubated in case to the spider silk fibroin nanofiber solution for forming translucent gels shape.
The length of spider silk fibroin nanofiber is 1 μm, a diameter of 10- in described spider silk fibroin nanofiber solution
20nm。
Described spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, 4.24g is added
Sodium carbonate and 5g spider silks, boil 30min, molten with 9.3mol/L LiBr solution after being cleaned up, drying with deionized water
Solution；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice,
Obtain spidroin solution.
Described spidroin solution is spider silk silk fibroin protein solution.
Described membranoid substance is prepared using following methods：By spidroin solution and spider silk fibroin nanofiber solution
Mixing, and the well mixed obtained spinning solution of paracetamol slow-release microcapsule, essential oil is added, spinning solution uses electrostatic spinning work
Three-D non-woven net-structured film is made in skill.
Described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin
Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film
Thickness is 50nm-20mm.
Embodiment 2
A kind of spider silk fibroin tissue renovation material, for the membranoid substance comprising spider silk fibroin, internal average cell density is
1-2000/mm2, average pore size is 1-300 μm, it is characterised in that described membranoid substance includes the raw material of following parts by weight：
90 parts of spidroin solution, 6 parts of spider silk fibroin nanofiber solution, 5 parts of paracetamol slow-release microcapsule, essential oil 2
Part.
Described paracetamol slow-release microcapsule is the water that the paracetamol of 3 mass parts is dissolved in 80 mass parts
In, and the hydrophilic emulsifier of 3 mass parts is added, and emulsion reaction is carried out in the presence of magnetic stirring apparatus and is made, described magnetic
The stir speed (S.S.) of power agitator is 1000-3000rpm, and emulsification times are 30-40min.
Described spider silk fibroin nanofiber solution is prepared using following methods：Spidroin solution is taken to be placed in 60 DEG C
The half of original volume is concentrated into insulating box, then be transferred in fume hood be concentrated into mass fraction for 30% concentrate, will concentrate
It is 0.5% that liquid, which is put into after 4 DEG C of refrigerator storage 2d concentrate being diluted with water mass fraction, and constant temperature is put into after full and uniform stirring
It is incubated in case to the spider silk fibroin nanofiber solution for forming translucent gels shape.
The length of spider silk fibroin nanofiber is 1 μm, a diameter of 10- in described spider silk fibroin nanofiber solution
20nm。
Described spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, 4.24g is added
Sodium carbonate and 5g spider silks, boil 30min, molten with 9.3mol/L LiBr solution after being cleaned up, drying with deionized water
Solution；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice,
Obtain spidroin solution.
Described spidroin solution is spider silk silk fibroin protein solution.
Described membranoid substance is prepared using following methods：By spidroin solution and spider silk fibroin nanofiber solution
Mixing, and the well mixed obtained spinning solution of paracetamol slow-release microcapsule, essential oil is added, spinning solution uses electrostatic spinning work
Three-D non-woven net-structured film is made in skill.
Described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin
Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film
Thickness is 50nm-20mm.
By tissue renovation material (SS) made from tissue renovation material (S) made from embodiment 1, embodiment 2, and by silk
The performance for the tissue renovation material (SSS) that fibroin albumen is made is contrasted, using with the small of identical degree of tissue damage
White mouse is tested, and material S, SS and SSS are spread on to the injury site of small white mouse respectively, is changed daily twice, continuously using 10
My god, using observation point system, small white mouse wounded tissue recovery extent is given a mark by 1-5 points respectively, every using effect is most
Excellent is 5 points, and the experimental data drawn is shown in Table 1.
The resultant effect of table 1 is investigated
| Material | S | SS | SSS |
| Tissue repair degree | 4.9 | 4.8 | 2.9 |
| Antiphlogistic effects | 4.8 | 4.9 | 2.6 |
By experimental result it can be seen that：Fibroin is substituted using spider silk fibroin, and added into spider silk fibroin albumen
Plus spider silk fibroin nanofiber paracetamol slow-release microcapsule and essential oil, the tissue that can effectively lift fibroin albumen repaiies
Reactivation power and antiphlogistic effects.
Claims (8)
1. a kind of spider silk fibroin tissue renovation material, for the membranoid substance comprising spider silk fibroin, internal average cell density is 1-
2000/mm2, average pore size is 1-300 μm, it is characterised in that described membranoid substance includes the raw material of following parts by weight：Spider
80-90 parts of spider's thread protein solution, 4-6 parts of spider silk fibroin nanofiber solution, 4-5 parts of paracetamol slow-release microcapsule,
1-2 parts of essential oil；
Described paracetamol slow-release microcapsule is that the paracetamol of 2-3 mass parts is dissolved in into 70-80 mass parts
In water, and the hydrophilic emulsifier of 1-3 mass parts is added, emulsion reaction is carried out in the presence of magnetic stirring apparatus and is made, it is described
Magnetic stirring apparatus stir speed (S.S.) be 1000-3000rpm, emulsification times are 30-40min.
2. a kind of spider silk fibroin tissue renovation material according to claim 1, it is characterised in that described spider silk protein
White nanofiber solution is prepared using following methods：Take spidroin solution to be placed in 60 DEG C of insulating boxs and be concentrated into original volume
Half, then be transferred in fume hood and be concentrated into mass fraction and obtain concentrate for 20-30%, concentrate is put into 4 DEG C of refrigerator storage 2d
It is 0.5% that concentrate is diluted with water into mass fraction afterwards, incubation in insulating box is put into after full and uniform stirring semi-transparent to being formed
Bright gelatinous spider silk fibroin nanofiber solution.
3. a kind of spider silk fibroin tissue renovation material according to claim 2, it is characterised in that：Described spider silk protein
The length of spider silk fibroin nanofiber is 1 μm, a diameter of 10-20nm in white nanofiber solution.
4. a kind of spider silk fibroin tissue renovation material according to claim 1, it is characterised in that described spider silk protein
White solution is prepared using following methods：2L deionized waters are heated to boiling, 4.24g sodium carbonate and 5g spider silks is added, boils
30min, after being cleaned up, drying with deionized water, is dissolved with 9.3mol/L LiBr solution；Solution is filled until completely dissolved
Enter bag filter, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, obtains spidroin solution.
5. a kind of spider silk fibroin tissue renovation material according to claim 1, it is characterised in that：Described spider silk protein
White solution is spider silk silk fibroin protein solution.
6. a kind of spider silk fibroin tissue renovation material according to claim 1, it is characterised in that described membranoid substance is adopted
Prepare using the following method：Spidroin solution is mixed with spider silk fibroin nanofiber solution, and adds acetparaminosalol
The netted knot of three-D non-woven is made using electrostatic spinning process in the well mixed obtained spinning solution of phenol slow-release microcapsule, essential oil, spinning solution
Structure film.
7. a kind of spider silk fibroin tissue renovation material according to claim 6, it is characterised in that described electrostatic spinning
Technique is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spinning solution flow 2-5ml/h, heating temperature
50-150 DEG C of degree.
8. a kind of spider silk fibroin tissue renovation material according to claim 6, it is characterised in that：Described three-D non-woven
Fibre diameter is 50nm-20um in net-structured film, and interfiber pore is l-500um, and film thickness is 50nm-20mm.
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| CN110028569A (en) * | 2019-05-06 | 2019-07-19 | 东华大学 | A kind of preparation method of natural spider silk albumen powder |
| CN110075353A (en) * | 2019-05-06 | 2019-08-02 | 东华大学 | A kind of preparation method of natural spider silk bio-ink |
| CN110172785A (en) * | 2019-06-05 | 2019-08-27 | 安徽迈德普斯医疗科技有限公司 | A kind of waterproof surgical paper |
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