CN108308403A - A method of correction feedstuff amino acid digestibility is prepared for pannage - Google Patents

Abstract

The invention discloses a method for correcting amino acid digestibility of feed raw materials for pig feed preparation, comprising: (1) installing a fistula at the end of the ileum of a growing pig to collect end ileal chyme of the animal, and measuring the end ileum digestibility of amino acids; 2) AID and SID were measured when growing pigs were fed semi-homogeneous diets under restricted feeding conditions; the dietary amino acid digestibility was measured when growing pigs were fed conventional diets under ad libitum conditions; The dietary intake FI and the concentration of nutrient components in the diet were used to establish the correction coefficient binary regression prediction equation of each amino acid digestibility: by combining the concentration of the amino acid in the semi-homozygous diet and the percentage of daily feed intake in the experimental pig body weight with The corrected digestibility is obtained by multiplying the obtained digestibility correction coefficient by the amino acid digestibility determined by the semi-homozygous diet; after verification, the corrected feed raw material amino acid digestibility value is used to prepare a full-price compound feed, and the obtained compound feed The digestible amino acid content was basically consistent with the actual measured value.

Description

A method for correcting feed raw material amino acid digestibility for swine feed preparation

technical field

The invention relates to a method for correcting amino acid digestibility of feed raw materials for preparing feed for growing pigs, and belongs to the technical field of feed.

Background technique

Accurately assessing the amino acid digestibility of feed ingredients and using it in feed preparation is the key research content of pig amino acid nutrition. According to the research progress of the amino acid assessment of feed ingredients, the amino acid digestibility of the terminal ileum can better represent the effective absorption of the protein nutrition of the feed raw materials by pigs. Therefore, half of the amino acid digestibility of the terminal ileum is currently used to estimate the digestible amino acid nutrients in the diet of growing and finishing pigs. Also, the amino acid requirements of growing and finishing pigs are usually expressed in terms of digestible nutrients in the terminal ileum. According to different correction methods for endogenous nitrogen excretion in pigs, amino acid digestibility in the terminal ileum can be divided into apparent (AID) and standard (SID) ileal amino acid digestibility.

The assessment of amino acid digestibility of feed ingredients for growing pigs is affected by crude protein level, diet type, and feed intake. At present, when carrying out digestion and metabolism experiments to determine the amino acid digestibility of feed ingredients for growing pigs, growing pigs are usually fed semi-homogeneous diets mainly composed of corn starch (the feed ingredients to be tested are the only source of protein in the diet), and the daily feed intake is controlled at About 4% of body weight (restricted feeding). The commercial feed fed in the pig production process is usually a conventional diet based on corn and soybean meal, and the average daily feed intake can reach 5% of the growing pig's body weight under the condition of ad libitum feeding. There must be some differences in amino acid digestibility measured under the condition of 4% feed intake using semi-homogeneous diets for the preparation of conventional diets based on corn and feeding growing pigs under the condition of ad libitum feeding. Therefore, when the amino acid digestibility measured by semi-homozygous rations under feed restriction conditions is actually used to formulate complete compound rations, there may be a phenomenon that the estimated digestible amino acid content in the ration does not match the actual measured value. In order to overcome this problem, the present invention makes the formulated feed closer to the amino acid requirement of growing pigs by establishing a method for correcting the amino acid digestibility of feed ingredients for growing pigs.

Contents of the invention

An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages as will be described hereinafter.

In order to achieve these objects and other advantages according to the present invention, a method for correcting feed material amino acid digestibility for swine feed preparation is provided, comprising the following steps:

Step 1, installing a fistula in the terminal ileum of the growing pig by surgical operation to continuously collect the terminal ileal chyme of the animal, so as to measure the terminal ileal digestibility of amino acids;

Step 2. Feed test pigs with semi-homogeneous rations under restricted feeding conditions, and measure the levels of corn, soybean meal, wheat flour, corn ethanol tank and its solubles and Apparent ileal amino acid digestibility and standard ileal amino acid digestibility of conventional dietary raw materials such as rapeseed meal; among them, the daily feed intake was calculated from 3-5% of pig body weight under restricted feeding conditions, and the daily feed intake under restricted feeding conditions The next day's feed intake is close to 5% of the pig's body weight;

Step 3. Establish the correction coefficient binary regression prediction equation of each amino acid digestibility with the concentration of nutrients in the diet and the feed intake FI as independent variables:

Arginine Arg: y=1.2824-0.3732Arg-0.0574FI+0.0749Arg×FI, R ² =0.9629, where the range of Arg concentration is 0.33-0.12%;

Histidine His: y=1.3840-1.2096His-0.0745FI+0.2320His×FI, R ² =0.9187, where the range of His concentration: 0.20-0.53%;

Ileucine Ile: y=1.3968-0.7221Ile-0.0765FI+0.1367Ile×FI, R ² =0.9012, where the range of Ile concentration is 0.30-0.75%;

Leucine Leu: y=1.4631-0.4493Leu-0.0890FI+0.0851Leu×FI, R ² =0.8951, where the range of Leu concentration is 0.69-1.50%;

Lysine Lys: y=1.4648-0.6348Lys-0.0942FI+0.1260Lys×FI, R ² =0.9044, where Lys concentration range: 0.44-1.15%;

Methionine Met: y=1.3595-2.4545Met-0.0709FI+0.4734Met×FI, R ² =0.8634, where the range of Met concentration: 0.09-0.29%;

Phenylalanine Phe: y=1.3499-0.5118Phe-0.0683FI+0.0982Phe×FI, R ² =0.9107, where the range of Phe concentration is 0.40-1.00%;

Threonine Thr: y=2.0128-2.0972Thr-0.2018FI+0.4158Thr×FI, R ² =0.9920, where the range of Thr concentration is 0.25-0.66%;

Tryptophan Trp: y=1.5159-3.4109Trp-0.0996FI+0.6518Trp×FI, R ² =0.9247, where the range of Trp concentration is 0.08-0.24%;

Valine Val: y=1.5190-0.8046Val-0.1019FI+0.1557Val×FI, R ² =0.9345, where Val concentration usage range: 0.36-0.95%;

Cystine Cys: y=1.7016-4.5423Cys-0.1400FI+0.8839Cys×FI, R ² =0.8668, where the range of Cys concentration: 0.10-0.31%;

Wherein, the feed intake FI is the percentage of the daily dry matter intake in the body weight of the experimental pig, which is 3-5%; the concentration of nutrients in the diet is the percentage of dry matter of each amino acid in the diet, that is, the concentration of the amino acid; By bringing the concentration of the amino acid in the semi-homozygous diet and the percentage of daily feed intake to the experimental pig body weight into the equation, the digestibility correction coefficient obtained is multiplied by the amino acid digestibility determined by the semi-homozygous diet. get corrected digestibility;

Step 4. Determining the dietary standard ileal amino acid digestibility of growing pigs fed corn-soybean meal, corn-DDGS feed, corn-rapeseed meal and corn-wheat secondary flour conventional diet under ad libitum conditions, verified by steps 3. Whether the calculated value of amino acid digestibility after establishing a mathematical model is consistent with the measured value;

Preferably, in the step 3, the binary regression prediction equation uses the PROC REG statement in the SAS software for model building.

The present invention at least includes the following beneficial effects: the present invention establishes the correction coefficient binary regression prediction equation of each amino acid digestibility by limiting the feed intake FI under feeding conditions and the concentration of nutrients in the diet, and through the binary regression prediction equation The corrected digestibility was obtained by multiplying the correction coefficient obtained by the amino acid digestibility determined by the semi-homozygous ration; after verification, the corrected amino acid digestibility value of feed raw materials was used to prepare a full-price compound feed, and the digestible amino acid content of the obtained compound feed was calculated to be equal to The actual measured values are basically consistent, and the accuracy is better than the amino acid digestibility of feed raw materials measured by the semi-homogeneous diet method commonly used in the academic circles.

Other advantages, objectives and features of the present invention will partly be embodied through the following descriptions, and partly will be understood by those skilled in the art through the study and practice of the present invention.

Detailed ways:

The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

Example 1:

In order to correct the amino acid digestibility of feed raw materials measured under the experimental conditions, in order to more accurately express the digestible amino acid content of the raw materials in the compound feed produced by pigs, we need to analyze the amino acid digestibility of the feed raw materials measured under the experimental conditions. rate correction;

A method for correcting feed raw material amino acid digestibility for pig feed preparation, comprising the following steps:

Step 1, installing a fistula in the terminal ileum of the growing pig by surgical operation to continuously collect the terminal ileal chyme of the animal, so as to measure the terminal ileal digestibility of amino acids;

Step 2. Feed test pigs with semi-homogeneous rations under restricted feeding conditions, and measure the levels of corn, soybean meal, wheat flour, corn ethanol tank and its solubles and Apparent ileal amino acid digestibility and standard ileal amino acid digestibility of conventional dietary raw materials such as rapeseed meal; among them, the daily feed intake was calculated from 3-5% of pig body weight under restricted feeding conditions, and the daily feed intake under restricted feeding conditions The next day's feed intake is close to 5% of the pig's body weight;

Step 3. According to the SID of different amino acids in conventional feed materials such as corn, soybean meal, wheat flour, corn ethanol tank and its solubles, and rapeseed meal measured in step 2, establish a mathematical model to correct the digestibility of different amino acids in feed materials The measured value, so that the sum after multiplying the corrected value of amino acid digestibility according to different feed raw materials by the correction coefficient is equal to the amino acid digestibility of the corresponding ration; There is a negative correlation with the feed intake under the grain amino acid concentration, and a positive correlation with the feed intake when the amino acid concentration is low; according to the test data of the present invention, using the PROC REG statement in the SAS software, the feed intake (FI, daily Dry matter intake accounted for the percentage of the experimental pig body weight, 3-5%), the concentration of nutrients in the diet (percentage of dry matter in the diet for each amino acid), and the establishment of correction coefficients for the digestibility of each amino acid (long fattening pigs, free Ingestion, meet the rations of its nutritional requirements) binary regression prediction equation and the scope of use of amino acid concentration are as follows:

Among them, the calculation process of the correction coefficient of lysine digestibility is illustrated as follows:

Using the soybean meal semi-homogeneous diet containing 1% lysine, the lysine SID was determined to be 85.1%, 85.0%, and 79.5% at the daily feed intake equivalent to 3%, 4%, and 5% of body weight; Using the soybean meal semi-homogeneous diet containing 0.5% lysine, the lysine SID was determined to be 76.1%, 80.3%, and 80.7% at the daily feed intake equivalent to 3%, 4%, and 5% of body weight; According to the experimental observation, the daily feed intake of the test pigs is about 5% of the body weight when they eat freely, so the data of 5% feed intake is used as the reference data in the case of free feeding, and the concentration of lysine in the diet is obtained. When the dietary lysine level was 1%, the lysine SID under the three feed intake levels were 93.4%, 93.5%, and 100% under the condition of ad libitum feeding respectively; The lysine SID under the feed intake level is 106.0%, 100.5%, and 100% under the free feeding condition respectively; if the percentage of the measured lysine SID equivalent to the lysine SID under the free feeding condition is y, the measured The lysine content of the semi-homozygous diet used at the time was X1 (Lys), and the percentage of feed intake equivalent to body weight was X2 (FI). Amino acid correction factor is:

Lysine Lys: y=1.4648-0.6348Lys-0.0942FI+0.1260Lys×FI, R ² =0.9044, where Lys concentration range: 0.44-1.15%

In the same way, the correction coefficients for various essential amino acids are established as follows:

Arginine Arg: y=1.2824-0.3732Arg-0.0574FI+0.0749Arg×FI, R ² =0.9629, where the range of Arg concentration is 0.33-0.12%;

Histidine His: y=1.3840-1.2096His-0.0745FI+0.2320His×FI, R ² =0.9187, where the range of His concentration: 0.20-0.53%;

Ileucine Ile: y=1.3968-0.7221Ile-0.0765FI+0.1367Ile×FI, R ² =0.9012, where the range of Ile concentration is 0.30-0.75%;

Leucine Leu: y=1.4631-0.4493Leu-0.0890FI+0.0851Leu×FI, R ² =0.8951, where the range of Leu concentration is 0.69-1.50%;

Methionine Met: y=1.3595-2.4545Met-0.0709FI+0.4734Met×FI, R ² =0.8634, where the range of Met concentration: 0.09-0.29%;

Phenylalanine Phe: y=1.3499-0.5118Phe-0.0683FI+0.0982Phe×FI, R ² =0.9107, where the range of Phe concentration is 0.40-1.00%;

Threonine Thr: y=2.0128-2.0972Thr-0.2018FI+0.4158Thr×FI, R ² =0.9920, where the range of Thr concentration is 0.25-0.66%;

Tryptophan Trp: y=1.5159-3.4109Trp-0.0996FI+0.6518Trp×FI, R ² =0.9247, where the range of Trp concentration is 0.08-0.24%;

Valine Val: y=1.5190-0.8046Val-0.1019FI+0.1557Val×FI, R ² =0.9345, where Val concentration usage range: 0.36-0.95%;

Cystine Cys: y=1.7016-4.5423Cys-0.1400FI+0.8839Cys×FI, R ² =0.8668, where the range of Cys concentration: 0.10-0.31%;

Among them, ten essential amino acids and one non-essential amino acid cystine are included; since pigs have a certain ability to synthesize non-essential amino acids in the body, the determination of the digestibility of non-essential amino acids in feed is affected by this variation; the regression equation It is difficult to establish and the accuracy is not high; but because non-essential amino acids can be synthesized in the pig body, it is not necessary to carefully consider the content of various non-essential amino acids in the diet during production, so it is not listed here; cystine Methionine is usually considered together with methionine in the actual formulation of growing and finishing pig diets, so the regression prediction equation is listed here.

When using the regression equation to correct amino acid digestibility, the concentration of the amino acid in the semi-homozygous diet and the percentage of daily feed intake in the experimental pig body weight should be brought into the equation, and the digestibility correction coefficient obtained should be multiplied by the semi-purity The amino acid digestibility determined by the combined diet is the corrected digestibility, which can more accurately estimate the digestible amino acid content of the full-price diet formulated with this feed material under the condition of pigs eating freely;

For example, using a semi-homozygous diet to measure soybean meal lysine SID is 87%, the dry matter content of lysine in the semi-homozygous diet is 0.88%, and the daily feed intake accounts for 3.5% of the experimental pig's body weight. Substitute into the formula:

y=1.4648-0.6348Lys-0.0942FI+0.1260Lys×FI,

Wherein Lys=0.88, FI=3.5, get:

y=1.4648-0.6348×0.88-0.0942×3.5+0.1260×0.88×3.5=0.9646, obtained from 87%×0.9646=83.9% of corrected soybean meal lysine digestibility in ad libitum compound feed;

As another example, the methionine digestibility of wheat flour was determined to be 68% using a semi-homozygous diet, the methionine content in the semi-homozygous diet was 0.10%, and the daily feed intake accounted for 3.2% of the experimental pig's body weight, which was substituted into the formula:

y=1.3595-2.4545Met-0.0709FI+0.4734Met×FI,

Wherein Met=0.10, FI=3.2, get:

y=1.3595-2.4545×0.10-0.0709×3.2+0.4734×0.10×3.2=1.0387, obtained from the corrected methionine digestibility of wheat flour in ad libitum compound feed 68%×1.0387=70.6%;

Step 4. Determining the dietary standard ileal amino acid digestibility of growing pigs fed corn-soybean meal, corn-DDGS feed, corn-rapeseed meal and corn-wheat secondary flour conventional diet under ad libitum conditions, verified by steps 3. Whether the calculated value of the corrected amino acid digestibility by establishing a mathematical model is consistent with the measured value; it has been verified that the digestible amino acid content of the calculated compound feed is basically consistent with the actual measured value. , the accuracy is better than the amino acid digestibility of feed ingredients obtained by using semi-homogeneous diets.

Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the specification and implementation, it can be applied to various fields suitable for the present invention, and it can be easily understood by those skilled in the art Therefore, the invention is not limited to the specific details and examples shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims (2)

1. a kind of correction feedstuff amino acid digestibility is used for the method that pannage is prepared, which is characterized in that including following step Suddenly：

Step 1: fistula is installed in grower pigs terminal ileum by surgical operation, continuously to collect the terminal ileum chyme of animal, To measure the ileal digestibility of amino acid；

Step 2: under the conditions of controlled feeding, with half homozygous Diet test pig, it is horizontal, no that different aminoacids are measured respectively With under feed intake level, the conventional diet raw material of corn, dregs of beans, wheat wheat-middlings, corn alcohol trough and its soluble matter and rapeseed dregs etc. Apparent ileal amino acid digestibility and standard ileal amino acid digestibility；Wherein, under the conditions of controlled feeding daily ingestion amount by The 3-5% of pig weight is calculated, and searches for food close to the 5% of pig weight day under the conditions of free choice feeding；

Step 3: establishing using nutritional ingredient concentration in diet and feed intake FI as the correction of each amino acid digestibility of independent variable Coefficient binary regression predictive equation：

Arginine Arg：Y=1.2824-0.3732Arg-0.0574FI+0.0749Arg × FI, R²=0.9629, wherein Arg is dense Spend use scope：0.33-0.12%；

Histidine：Y=1.3840-1.2096His-0.0745FI+0.2320His × FI, R²=0.9187, wherein His is dense Spend use scope：0.20-0.53%；

Isoleucine Ile：Y=1.3968-0.7221Ile-0.0765FI+0.1367Ile × FI, R²=0.9012, wherein Ile Concentration use scope：0.30-0.75%；

Leucine Leu：Y=1.4631-0.4493Leu-0.0890FI+0.0851Leu × FI, R²=0.8951, wherein Leu is dense Spend use scope：0.69-1.50%；

Lysine Lys：Y=1.4648-0.6348Lys-0.0942FI+0.1260Lys × FI, R²=0.9044, wherein Lys is dense Spend use scope：0.44-1.15%；

Methionine Met：Y=1.3595-2.4545Met-0.0709FI+0.4734Met × FI, R²=0.8634, wherein Met is dense Spend use scope：0.09-0.29%；

Phenylalanine Phe：Y=1.3499-0.5118Phe-0.0683FI+0.0982Phe × FI, R²=0.9107, wherein Phe Concentration use scope：0.40-1.00%；

Threonine Thr：Y=2.0128-2.0972Thr-0.2018FI+0.4158Thr × FI, R²=0.9920, wherein Thr is dense Spend use scope：0.25-0.66%；

Tryptophan Trp：Y=1.5159-3.4109Trp-0.0996FI+0.6518Trp × FI, R²=0.9247, wherein Trp is dense Spend use scope：0.08-0.24%；

Valine Val：Y=1.5190-0.8046Val-0.1019FI+0.1557Val × FI, R²=0.9345, wherein Val is dense Spend use scope：0.36-0.95%；

Cystine Cys：Y=1.7016-4.5423Cys-0.1400FI+0.8839Cys × FI, R²=0.8668, wherein Cys is dense Spend use scope：0.10-0.31%；

Wherein, feed intake FI is that daily dry matter intake accounts for experiment pig percentage of body weight, takes 3-5%；Nutritional ingredient in diet Dry matter percentage of a concentration of each amino acid in diet, the i.e. concentration of the amino acid；By that will be somebody's turn to do in half homozygous daily ration Daily ingestion amount accounts for experiment pig percent weight and brings equation into when the concentration of amino acid and experiment, is corrected using the digestibility obtained Coefficient is multiplied by the amino acid digestibility that half homozygous daily ration measures and obtains correction digestibility；

Step 4: measure grower pigs feeds Corn-soybean, corn-DDGS feeds, corn-rapeseed dregs under the conditions of free choice feeding With daily ration standard ileal amino acid digestibility when corn-wheat wheat-middlings routine diet, verify through step 3 founding mathematical models Whether the amino acid digestibility calculated value after correction coincide with measured value.

2. correction feedstuff amino acid digestibility is used for the method that pannage is prepared as described in claim 1, feature exists In in the step 3, binary regression predictive equation uses the PROCREG sentences in SAS softwares to carry out model foundation.