CN109893473B - Deep Ocean Concentrate Skin Care and Maintenance Kit and Its Application - Google Patents

Abstract

The present invention relates to a deep-ocean concentrated skin care and maintenance kit containing a deep-ocean concentrate and a skin care and maintenance formula. The deep-ocean concentrate skin care and maintenance kit of the present invention has: 1. Immediately micronized skin care and maintenance formula; 2. Immediately reduce the high surface tension of the deep-ocean concentrate, and solve the problem that the skin is not easily absorbed; 3. The deep-ocean concentrate One of its material properties becomes the driving force for the penetration of the micronized skin care and maintenance formula, making the skin (dermis) easier to absorb. The present invention further provides a method for preparing a deep ocean concentrate skin care and maintenance kit resulting in improved skin appearance.

Description

Deep Ocean Concentrate Skin Care and Maintenance Kit and Its Application

【Technical field】

The present invention relates to a deep-ocean concentrate skin care and maintenance kit containing a deep-ocean concentrate and a skin care and maintenance formula, and in particular to a composition of the deep-ocean concentrate and skin care and maintenance formula formulated in real time, which are micronized Molecular clusters reduce the high surface tension of the deep ocean concentrate, allowing the high concentration of charged ions within it to act as a driving force for permeability, which in turn facilitates the absorption of skin care and maintenance formulas by skin cells.

【Background technique】

Deep-sea water (DSW) is obtained from low-temperature, clean and pollution-free seawater below 200 meters above sea level. Deep-sea water is rich in magnesium, calcium, potassium and other metal ions, nutrients, and has Inorganic constituents of state and refractory properties. The reason why deep ocean water is rich in minerals is mainly because sunlight cannot penetrate and photosynthesis cannot occur, so that inorganic nutrients are not consumed by phytoplankton. Compared with surface seawater, deep seawater has a higher content of inorganic nutrients. Due to its rich inorganic nutrients, deep ocean water is currently widely used in health drinks, food, pharmaceutical products and aquaculture.

In recent years, deep ocean water has been widely used in food processing industry, agriculture, and medicine. Some research literature points out that deep ocean water can reduce blood fat, lipid oxidation, vascular sclerosis and hypertension, and can also reduce vascular wall hyperplasia; although in the study of atopic dermatitis, it was also found that patients drinking or soaking Deep sea water can improve the symptoms of skin inflammation and allergies, but it has not been widely used in the cosmetics industry. In addition, drinking deep ocean water has also been found to improve cataracts.

Since deep ocean water can only be collected from four places in the world, Taiwan, China has successfully collected and made a concentrate, which can be eaten and used as a skin care product. It has been pointed out in the cell experiments that deep ocean water can stimulate collagen regeneration, whiten the skin, and inhibit or treat inflammation. However, deep ocean water has not been widely used in cosmetic and skin care products. Generally, commercial skin care products need to use emulsifiers to stably fuse oil and water, so the molecular clusters are large and difficult to be absorbed by the skin, while the deep ocean concentrate has a balanced and stable ionic composition, which is easily absorbed by cells, but has high surface tension. properties that make it less likely to penetrate into the skin. There is still no literature that discloses an effective product that directly mixes skin care products with deep ocean concentrates. In order to provide more effective skin care products and at the same time increase the industrial value of deep ocean water, the industry urgently needs to provide a composition containing deep ocean concentration (liquid/powder) and a skin care and maintenance formula.

[Content of the invention]

In view of this, the present invention provides a skin care and maintenance formula of a skin care and maintenance kit, a method of using the same, and a method of making the same. And the deep-sea concentrate skin care and maintenance kit of the present invention is characterized in that it has immediate micronization skin care and maintenance formula, immediately reduces the high surface tension of the deep-sea concentrate, solves the problem that the skin is not easily absorbed, and the substance of the deep-sea concentrate The characteristic can become the driving force (Driving Force) for the penetration of micronized skin care and maintenance formula, making the skin (dermis layer) easier to absorb.

The present invention provides a skin care and maintenance kit of deep ocean concentrate, comprising a first agent and a second agent, the first agent is the deep ocean concentrate, the second agent is a skin care and maintenance formula, and the two are individual package.

The present invention provides a deep-sea concentrate skin care and maintenance kit, wherein the magnesium concentration of the deep-sea concentrate is 6,500-110,000 mg/l.

The present invention provides a skin care and maintenance kit of deep ocean concentrate, wherein the sodium concentration of the deep ocean concentrate is 3,800-30,000 mg/l, the potassium concentration is 400-18,000 mg/l, and the calcium concentration is 100-500 mg/l l.

The present invention provides a skin care and maintenance kit of deep sea water concentrate, wherein the hardness of the deep sea water concentrate is 80,000-420,000 mg/l, and the salinity is 380-430‰.

The present invention provides a skin care and maintenance kit with deep sea water concentrate, wherein the deep sea water concentrate contains 1,000-1,500 mg of calcium, 1,400-2,100 mg of magnesium, 2,000-4,000 mg of sodium, 1,400-2,100 mg of sodium per 100 g of the deep-sea sea water concentrate The solution of marine minerals with potassium and sulfate <6.5wt% and specific gravity of 1.17-1.32g/ ^cm3 .

The present invention provides a skin care and maintenance kit of deep sea water concentrate, wherein the deep sea water concentrate is taken from a water source below 200m in the ocean.

The present invention provides a skin care and maintenance kit of deep sea water concentrate, wherein the deep sea water concentrate is taken from a water source below 500m in the ocean.

The present invention provides a skin care and maintenance kit of deep ocean concentrate, wherein the skin care and maintenance formulations are whitening products, moisturizing products, wrinkle-removing products, wound healing products, medical or cosmetic introduction products, collagen hyperplasia promotion products, etc. All products related to improving the appearance of the skin.

The present invention further provides a deep-sea concentrate skin care and maintenance kit, wherein the deep-sea concentrate is liquid or powder.

The present invention further provides a deep-ocean concentrate skin care and maintenance kit, wherein the skin care and maintenance formula is in the form of gel or cream.

The present invention further provides a method of promoting penetration of a skin care formulation, (a) providing a skin care formulation; (b) mixing the skin care formulation with a deep ocean concentrate prior to use; The skin care and maintenance formula is immediately micronized to increase the penetration of the skin care and maintenance formula, and the skin care and maintenance formula can quickly enter the skin and reach the dermis layer.

The present invention further provides a method of promoting penetration of a skin care and maintenance formulation, wherein the magnesium concentration of the deep ocean concentrate is 6,500-110,000 mg/l.

The present invention further provides a method for promoting penetration of skin care and maintenance formulations, wherein the concentration of sodium in the deep ocean concentrate is 3,800-30,000 mg/l, the concentration of potassium is 400-18,000 mg/l, and the concentration of calcium is 100 mg/l. ~500mg/l.

The present invention further provides a method for promoting penetration of skin care and maintenance formulations, wherein the hardness of the deep ocean concentrate is 80,000-420,000 mg/l, and the salinity is 380-430‰.

The present invention further provides a method for promoting penetration of skin care and maintenance formulations, wherein the deep ocean concentrate is liquid or powder.

The present invention further provides a method for promoting penetration of a skin care and maintenance formulation, wherein the skin care and maintenance formulation is in a gel or cream form.

The present invention further provides a method for promoting penetration of a skin care and maintenance formulation, wherein after the deep ocean concentrate is mixed with the skin care and maintenance formulation, the effective concentration of the deep ocean concentrate is at least greater than or equal to 1.25 (v/v)%.

The present invention further provides a method of promoting penetration of a skin care and maintenance formulation, wherein the deep ocean concentrate is mixed with the skin care and maintenance formulation and applied to an affected area immediately.

The present invention further provides a method for promoting the penetration of a skin care and maintenance formulation, wherein the affected part is the skin.

The present invention further provides a method for skin care and maintenance, which comprises administering the above-mentioned deep ocean concentrate skin care and maintenance kit to a skin immediately after the preparation is completed.

The present invention further provides a method for skin care and maintenance, wherein after the deep ocean concentrate is mixed with the skin care and maintenance formulation, the effective concentration of the deep ocean concentrate is at least greater than or equal to 1.25 (v/v)% .

The present invention further provides a method for skin care and maintenance, wherein the deep ocean concentrate skin care and maintenance kit can quickly enter the skin, reach the dermis layer, and achieve the effects of wrinkle removal, whitening, firming, etc. to improve the appearance of the skin .

To achieve the above and other objects, one or more specific embodiments of the present invention are described below. Other features or advantages of the present invention are described in detail in the embodiments and claims.

【Description of drawings】

Figures 1A-1C show the changes before and after the application of the "Deep Ocean Concentrate Skin Care and Maintenance Kit" to the wound, wherein Figure 1A is a photo of the wound before application. Figure 1B is a photo of a wound just after application of the Deep Ocean Concentrate Skin Care Kit. Figure 1C shows the wound several hours after application of the Deep Ocean Concentrate Skin Care Kit.

Figures 2A-2G show images taken with 40x and 200x optical microscopes to observe deep ocean concentrate (Figure 2A), maintenance gel (Figure 2B), maintenance cream (Figure 2C-1, 2C-2), real-time mixing Deep Ocean Concentrate and Maintenance Gel (Deep Ocean Concentrate Volume: Maintenance Gel Volume = 1:1) (Fig. 2D-1, 2D-2), Real-Time Mixing Deep Ocean Concentrate and Maintenance Cream (Deep Ocean Concentrate Volume: Maintenance Cream Volume = 1:1) (Figures 2E-1, 2E-2), Mixed Deep Ocean Concentrate and Maintenance Gel for 3 hours (Deep Ocean Concentrate Volume: Maintenance Gel Volume = 1:1 ) (Fig. 2F-1, 2F-2), mixed deep ocean concentrate and maintenance cream for 3 hours (deep ocean concentrate volume: maintenance cream volume = 1:1) (Fig. 2G-1, 2G-2 ).

Figures 3A-3C show changes in physical properties after 10 days of mixing the deep ocean concentrate with the skin care maintenance formula. Figure 3A shows that after 10 days of mixing the deep ocean concentrate with the skin care gel, a white lumpy precipitate was produced. Figure 3B shows that after 10 days of mixing the deep ocean concentrate with the maintenance gel, the gel disintegrated and the viscosity of the gel was significantly reduced. Figure 3C shows that after 10 days of mixing the deep ocean concentrate with the maintenance cream, the cream disintegrated and the viscosity of the cream was significantly reduced.

Figures 4A-4B show the introduction of the "Deep Ocean Concentrate Skin Care and Maintenance Kit" into the patient's face with an iontophoresis device to improve facial texture and freckles.

5A-5B show dynamic laser particle size analysis diagrams, wherein FIG. 5A is an analysis diagram of the particle size of the deep ocean concentrate; FIG. 5B is a real-time mixing of the deep ocean concentrate and the maintenance gel (weight of the deep ocean concentrate: weight of the maintenance gel =1:1) particle size analysis diagram.

Figure 6A shows a graph of particle size changes at different time points after real-time mixing of deep ocean concentrate and maintenance gel (weight of deep ocean concentrate: weight of maintenance gel = 1:1).

Figure 6B shows a particle size microscope view of the maintenance gel.

Figure 6C shows a microscopic view of particle size after mixing pure water and maintenance gel (pure water: maintenance gel weight = 1:1).

Figure 6D shows a microscopic view of micelle particles mixed with deep ocean concentrate and maintenance gel (deep ocean concentrate weight: maintenance gel weight = 1:1).

【Detailed ways】

The present invention provides a skin care and maintenance set of deep ocean concentrates.

The "deep ocean water" mentioned in the present invention refers to the sea water taken from 200 to 1500 meters below the sea level, preferably 500 to 700 meters below the sea level. 1500mg of calcium, 1400-2100mg of magnesium, 2000-4000mg of sodium, 1400-2100mg of potassium, sulfate <6.5wt%, and a solution of marine minerals with a specific gravity of 1.17-1.32g/ ^cm3 .

The "deep-ocean concentrate" mentioned in the present invention refers to the liquid or powder produced by the concentration of deep-ocean water. This deep ocean concentration (liquid/powder) concentration procedure uses steps such as fiber filtration system, ultrafiltration system, seawater reverse osmosis system, low temperature vacuum evaporation concentration system and/or centrifugation.

The deep ocean concentrate has a hardness of 80,000 to 420,000 mg/l, a salinity of 380 to 430‰, a magnesium concentration of 6,500 to 110,000 mg/l, and a sodium concentration of 3,800 to 30,000 mg/l. The deep ocean concentrates of the present invention are rich in various minerals including, but not limited to, calcium (Ca), potassium (K), iron (Fe), zinc (Zn), molybdenum (Mo), manganese (Mn), Trace elements such as lithium (Li), strontium (Sr), copper (Cu), and silicon (Si). In one embodiment, the composition of the deep-ocean concentrate is shown in Table 1, but since the detection unit has not completed the complete element detection, the deep-ocean concentrate of the present invention is not limited to this.

Table 1. Composition of deep ocean concentrates

The skin care and maintenance formula described in the present invention refers to the formula used in the course of treatment in general skin care products and beauty-related institutes, and can be a cream or gel skin care product. For example, whitening products, moisturizing products, anti-wrinkle products, wound healing products, quick-healing treatments and other products that improve the appearance of the skin.

"Mixtures of deep ocean concentrates and skin care maintenance formulations" or "deep ocean concentrate mixtures" of the present invention refer to admixtures of combined deep ocean concentrates and skin care maintenance formulations. After the marine concentrate is added to gel-like or cream-like skin care products, phase transfer can occur immediately, making the molecules smaller, so that skin care products can be easily absorbed by the skin and reach the deep layers of the skin to exert their effects.

However, deep ocean concentrates can cause skin care formulations to deteriorate, become unstable and disintegrate after being added to skin care formulations for a period of time. Therefore, the mixture of the deep ocean concentrate of the present invention and the skin care and maintenance formula is preferably freshly formulated, within 10 days after mixing, preferably 9 days, 8 days, 7 days, 6 days, 5 days, 4 days, 3 days Use within 1 day, 2 days and 1 day, preferably within 12 hours, 6 hours, 3 hours, 2 hours and 1 hour.

When the deep ocean concentrate is mixed with the skin care formula, the surface tension of the mixture (deep sea concentrate and skin care formula) can be reduced and the absorption of the skin care kit by the skin (dermis layer) is increased. Therefore, the mixture is easily absorbed by the skin and reaches the dermis layer to exert its effect.

The present invention further provides a method of promoting penetration of a skin care and maintenance formulation comprising providing a skin care and maintenance formulation which is mixed with a deep ocean concentrate prior to use.

The mixing method is not particularly limited, and general methods can be used, such as stirring, shaking, test tube shaking, ultrasonic shaking, etc., as long as the two can be uniformly mixed. The mixing time is not particularly limited, because it must be used as soon as possible after mixing to avoid the deterioration of the quality of the skin care and maintenance formula, so the mixing time should not be too long, usually within 15 minutes, preferably 14, 13, 12, 11, 10 , 9, 8, 7, 6, 5, 4, 3 minutes. The mixing time can be adjusted appropriately according to the skin care and maintenance formula used and the mixing method.

As mentioned above, the Deep Ocean Concentrate must be applied to the skin as soon as possible after mixing with the skin care formula to avoid changes in the quality of the skin care formula.

Only 10 days after the Deep Ocean Concentrate was added to the skin care formula, the gel and cream care products had deteriorated significantly. In addition, deep-ocean concentrates will cause unidentified sediments and floating substances in gel skin care products, indicating that if deep-ocean concentrates are directly added to skin care and maintenance formulas for a period of time, their ions will chemically change with the ingredients, reducing the original There are skin care and maintenance formulas that have the effect on the skin, and even produce derivatives that may be harmful to the skin. Therefore, deep-ocean concentrates and skin care products must be packaged separately and freshly prepared before use to achieve a mutually reinforcing effect.

After being added to the skin care and maintenance formula, the deep ocean concentrate can immediately produce a phase transfer, which reduces the molecules of the skin care product, so that the skin care and maintenance formula can be easily absorbed by the skin and reach the dermis. Therefore, the deep ocean concentrate can promote the penetration of the commercial skin care and maintenance formula into the skin, and accelerate the effect of the skin care and maintenance formula.

The present invention further provides a method of skin care, comprising administering the deep ocean concentrated mixture of the present invention to the skin immediately after preparation. After being smeared or introduced into the skin through an instrument, the deep-ocean concentrated mixture of the present invention can quickly reach the interior of the skin, be absorbed by the body, and exert its effect.

【Example】

Preparation method of deep ocean concentrate:

The preparation steps of the deep ocean concentrate include the following steps: firstly providing deep seawater at a predetermined depth from the sea level, and sequentially filtering the deep seawater by using a fiber filtration system, an ultrafiltration system and a seawater reverse osmosis system, so that the first concentrated solution and pure water are obtained, By concentrating the first concentrated liquid through a low temperature vacuum evaporation concentration system, the second concentrated liquid and calcium sulfate (gypsum) can be obtained. Because the low-temperature vacuum evaporation and concentration system is a circulation system, the second concentrated liquid can be continuously concentrated by its system. During the concentration process, the second concentrated solution will produce crystalline salts, so the solid particulate salt (ie deep sea salt) and the third concentrated solution can be obtained by centrifugation. The third concentrated solution is heated at a predetermined high temperature (90-120° C.), during the heating process, the third concentrated solution will continue to have crystalline salts to separate out, and the heated third concentrated solution will be allowed to stand, so that the crystalline salts are precipitated in the lower layer, and The supernatant was drawn to obtain the fourth concentrated solution. Finally, the cooled fourth concentrate is filtered with a filter membrane having a predetermined pore size (0.5˜1.5 μm) to obtain a deep seawater concentrate.

According to the material properties of different formulations of deep-sea concentrates and the properties of care and maintenance formulas, a deep-sea concentrated mixture with different functions is prepared, which can optimize the whitening, moisturizing, wrinkle-removing, wound healing and other effects of the mixture.

Various formulations of deep ocean concentration (liquid/powder) are prepared by the above-mentioned process method, and a reasonable interval is selected from each formulation, as shown in Table 2 below.

Table 2. Ingredient content of deep-sea concentrates (liquid/powder) with different formulas

Example 1: Wound experiment

On the injured leg, apply the "Real-time Mix Deep Ocean Concentrate and Maintenance Gel" mixture and observe the changes in the wound.

Referring to Figures 1A-1C, the leg was injured at 10:16 am on September 23, 2017 (see Figure 1A), the wound was disinfected at 3:56 pm and the ocean of "Real-time Mixed Deep Ocean Concentrate and Maintenance Gel" was applied For the deep concentrated (liquid/powder) skin care and maintenance kit (as shown in Figure 1B), at 10:45 in the evening, the redness and swelling of the wound subsided without scab formation (as shown in Figure 1C).

From the results, it can be seen that mixing the maintenance gel with the deep ocean concentrate can accelerate skin absorption and promote wound healing.

Embodiment 2: the influence of deep-sea concentrated liquid on the penetration of maintenance cream and gel

Use a dosing dropper to pipette an equal mixture of Deep Ocean Concentrate and Cream or Gel. Control groups were (a) water, (b) deep ocean concentrate, (c) gel, or (d) cream. The experimental group is 1mL deep ocean concentrate (1 times, 5 times, 10 times, 20 times, 40 times) and 1mL cream or gel, the effective concentration in the final mixture of deep ocean concentrate: 50(v/v)%, 10 (v/v)%, 5(v/v)%, 2.5(v/v)% and 1.25(v/v)%.

On the penetration test regular cloth AATCC97, 0.5 mL of the mixture was dropped on the regular cloth AATCC97 at a height of a fixed distance of 1 cm. After the "Deep Sea Concentrate and Gel" mixture was added, start counting the time it took for the mixture to penetrate the test cloth completely; on the other hand, after the "Deep Sea Concentrate and Cream" mixture was added, start timing 2 The permeation of the mixture was observed within minutes, and the results are shown in Table 3.

Table 3. Comparison of penetrating power before and after mixing skin care products with deep ocean concentrate

According to the experimental results of (b) deep-sea concentrated liquid, the deep-sea concentrated liquid in ionic state, although many literatures have proved that it has whitening and anti-aging effects on skin cells, just like many other maintenance ingredients, in fact, sea water Deep ocean concentrates have high surface tension and have difficulty absorbing and penetrating skin cells.

However, after mixing the deep ocean concentrate with the maintenance gel or maintenance cream in a volume of 1:1, the molecular clusters can be reduced, the penetration and absorption of the skin care products can be improved immediately, and the amount of the deep ocean concentrate can also be immediately reduced. High surface tension to solve the problem of skin absorption. In order to produce excellent penetration rate while increasing the absorption rate of the skin and achieve better results.

Example 3. Effect of Deep Ocean Concentration on Skin Care and Maintenance Formula Size

This experiment was commissioned by the Institute of Materials and Chemical Engineering of the Industrial Technology Research Institute to conduct the experiment, photographed with an optical microscope, and observed the deep ocean concentrate (as shown in Fig. 2A), maintenance gel (as shown in Fig. Mix Deep Ocean Concentrate and Maintenance Gel (Deep Ocean Concentrate Volume: Maintenance Gel Volume = 1:1) (Figure 2D), Real-time Mix Deep Ocean Concentrate and Maintenance Cream (Deep Ocean Concentrate Volume: Maintenance Cream volume = 1:1) (Figure 2E), leave for 3 hours to mix deep ocean concentrate and maintenance gel (deep ocean concentrate volume: maintenance gel volume = 1:1) (Figure 2F), leave A 3-hour mix of Deep Ocean Concentrate and Maintenance Cream (Deep Ocean Concentrate Volume: Maintenance Cream Volume = 1:1) (Figure 2).

Observed under a microscope with a magnification of 40, the molecular clusters of the maintenance gel (as shown in Figure 2B) and the maintenance cream (as shown in Figure 2C-1) are compact, and there are no granular particles. Observing the maintenance cream at a multiple of 200 (as shown in Figure 2C-2), there is no obvious granularity.

Observe under the microscope at 40 and 200 magnifications, mix deep ocean concentrate and maintenance gel in real time (as shown in Figure 2D-1, Figure 2D-2) and real-time mixing of deep ocean concentrate and maintenance cream (as shown in Figure 2E-1) , as shown in Figure 2E-2), the maintenance gel and maintenance cream with dense molecular clusters are immediately micronized by charged mineral ions to one-third or less of the original and evenly distributed. To sum up, real-time mixing of Deep Ocean Concentrate and Maintenance Gel/Cream, a clear encapsulation and even mixing of oil and water will make it easier for the skin to absorb.

However, under the microscope at 40 and 200 magnifications, the mixed deep ocean concentrate and maintenance gel (as shown in Figure 2F-1, Figure 2F-2) left for 3 hours and the mixed deep ocean concentrate and maintenance for 3 hours Cream (as shown in Figure 2G-1, Figure 2G-2), the molecular clusters have become larger, and the mixed deep-ocean concentrate and maintenance gel placed for 3 hours and the mixed marine solution placed for 3 hours are observed under a microscope with a magnification of 200. In deep concentrates and maintenance creams (as shown in Figure 2F-2 and Figure 2G-2), the phenomenon of molecular clusters can be found, which will be more difficult to be absorbed by the skin.

It can be known from the above experiments that the deep-sea concentrated solution of this case has the effect of micronizing the skin care and maintenance formula immediately. The separate maintenance gel and maintenance cream are macromolecules with tight molecular clusters, which are not easily absorbed by the skin. Mixed Deep Ocean Concentrate and Maintenance Gel/Cream that have been left for a while will have molecular clumps that make it harder to absorb. Only real-time mixing of Deep Ocean Concentrate and Maintenance Gel/Cream allows the respective molecules to be micronized and evenly mixed for easier absorption into the skin.

Example 4. Effect of deep ocean concentrate on the consistency and stability of maintenance creams and gels

Fill 1mL of cream and gel into microcentrifuge tubes respectively, centrifuge at 2000rpm for 5 minutes, and use sterilized ultrapure water to serially dilute the deep ocean concentrate by 1, 5, 10, 20 and 40 times. . Take 1mL of different dilution ratios of deep-sea concentrated solution and add them into microcentrifuge tubes that have been filled with 1mL of cream or gel and mix. The control groups were (a) 1mL ultrapure water + 1mL gel, (b) 0.5mL ultrapure water + 0.5mL cream; the experimental groups were 1mL deep ocean concentrated solution (1 times, 5 times, 10 times, 20 times times and 40 times) + 1 mL of cream or gel. The effective concentrations in the deep ocean concentrate final mixture were 50(v/v)%, 10(v/v)%, 5(v/v)%, 2.5(v/v)% and 1.25(v/v)%. The mixture was vigorously mixed with a test tube shaker, placed on a test tube rack and left to stand at room temperature for 10 days, and the viscosity of the mixture was measured with a glass capillary. Fix the microcentrifuge tube containing the mixture on the table, take a glass capillary and go deep into the microcentrifuge tube and time for 1 minute, take out the capillary, and measure the height of the fluid in the tube.

Referring to Figure 3A, the viscosity of the gel has changed significantly 10 days after mixing the deep ocean concentrate and the maintenance gel, which means that the physical properties have been denatured, and white lumps can be clearly seen under the naked eye. Chemical changes occurred during 10 days of standing.

Referring to FIG. 3B , after the deep-ocean concentrate is mixed with the maintenance cream, the viscosity of the cream can be found to be significantly reduced by naked eyes and experimental tests, which means that the stability of the cream has been changed.

Referring to Figure 3C, 10 days after mixing the deep-ocean concentrate with the maintenance gel, it can be found that the gel disintegrates and the consistency decreases by 100%. From Figure 3C, it can be found that when there is no deep-ocean concentrate, the capillary height is 1.5 cm, representing its It is the colloidal phase; and when the deep-ocean concentrate with high or low concentration is added, or even the deep-ocean concentrate that has been diluted 40 times (the concentration of the deep-ocean concentrate is 1.25%), the capillary height is 3cm, which means that it has become water box.

From the above, it can be seen that the deep ocean concentration (liquid/powder) will cause the oil-water separation of the skin care and maintenance formula to be unstable, and even long-term storage may produce a qualitative change in chemical crystallization, which cannot be confirmed to be beneficial to skin health. Therefore, the deep ocean concentrate and maintenance cream Or the gel cannot be stored for a long time after mixing. The best way to use it is to mix an equal amount and apply it to the skin before use.

Example 5: Real-time mixing of marine concentrate and maintenance gel non-invasive water light needle introduction test

On October 15, 2017, using a medical-grade iontophoresis device, a mixture of real-time mixing marine concentrate and maintenance gel was introduced into the right face. On October 26, the right face was imported again. After being introduced into the right face on October 15, the right face became red. The appearance of the skin indicates that the high-concentration ions in the deep ocean concentrate penetrated into the dermis and even the basal layer after the introduction, causing local microvascular blood circulation in the deep skin. The amount increased and the skin temperature increased slightly, which effectively promoted the skin metabolic rate, the redness disappeared within a few hours, and the skin texture was firm and smooth (Figure 4A). On October 26, the right face was imported again. Before and after the introduction, the right face was obviously lifted and the eyebrow height changed (as shown in Figure 4B). The proportions of facial texture, surface spots, purpurin and brown spots before and after the introduction were analyzed, as shown in Table 4.

Table 4. Comparison of facial skin before and after introduction

October 15 October 26 Change texture 51% 61% 10% superficial plaque 88% 91% 3% purpurin (deep spots) 89% 92% 3% Brown spot (basal) 47% 67% 20%

From the experimental results in the table above, it can be seen that using an iontophoresis device into the deep layers of cells can affect the effect of improving skin firmness, reducing fine lines and skin radiance after 24 hours. The material properties of the deep ocean concentrate in this case will become the driving force (Driving Force) for the penetration of the skin care and maintenance formula after micronization, making the skin (dermis) easier to absorb, and indeed reaching the basal layer for rapid repair and rapid repair. , Immediately increase the water retention capacity of the stratum corneum, and significantly improve the efficacy of the culture.

It can be seen from the above that compared with using the maintenance gel alone, the maintenance gel mixed with the marine concentrate can accelerate the skin absorption, quickly reach the dermis and even the basal layer, and produce a therapeutic effect.

Example 6. Effect of Deep Ocean Concentrate on Maintenance Gel Particle Size

This experiment was commissioned by the Institute of Materials and Chemical Engineering of the Industrial Technology Research Institute. The particle size was measured by a dynamic light scattering particle size analyzer, and the deep-sea concentrate (as shown in Figure 5A), the real-time mixing of the deep-sea concentrate and the maintenance gel ( Deep ocean concentrate weight: maintenance gel weight = 1:1) (Figure 5B).

The dynamic light scattering particle size analyzer uses laser light to inject into the solution containing particles. When the laser light hits the particles, scattered light is generated, and the particle size distribution of the particles is calculated by measuring the change of the scattered light with time.

According to the dynamic laser particle size analysis chart, the deep-ocean concentrate (as shown in Figure 5A) has the highest particle size distribution with a particle size of 0.89nm, and the overall average particle size is 19.2nm (Z average=19.2nm). The specimen is a liquid without significant particles.

According to the dynamic laser particle size analysis chart, after real-time mixing of deep ocean concentrate and maintenance gel (weight of deep ocean concentrate: weight of maintenance gel = 1:1) (as shown in Figure 5B), microcellular particles began to form , the main particle distribution according to the analysis results shows that 5082nm has the highest signal value, and the overall average particle size is 5.2μm (Z average=5.2μm). It can be seen from Fig. 5B that the signal value distribution of this sample is single and concentrated, indicating that the deep-sea concentrate and maintenance gel are mixed in real time (weight of deep-ocean concentrate: weight of maintenance gel = 1:1), which can be formed immediately. Uniformly sized micelle particles.

Table 5. Proportion distribution table of dynamic laser particle size analysis of deep ocean concentrate and real-time mixed deep ocean concentrate and maintenance gel

Example 7. Effect on particle size within a short period of time after real-time mixing of deep ocean concentrate and maintenance gel

This experiment is entrusted to the Institute of Materials and Chemical Engineering of ITRI to conduct the experiment, using optical microscope and dynamic light scattering particle size analyzer to observe the particle size of the sample. : Particle size changes at different time points after maintenance gel weight = 1:1) (Fig. 6A).

Under a 200-fold optical microscope, observe the deep-sea concentrate and maintenance gel (deep-ocean concentrate weight: maintenance gel weight = 1:1) with a fixed field of view at 0 seconds, 10 seconds, 20 seconds, 30 seconds after real-time mixing Second, 1 minute, 2 minutes, 3 minutes, 5 minutes, observe the sample with a fixed field of view and find that from the 0 second to the 5th minute after the real-time mixing of the deep ocean concentrate and the maintenance gel, the deep ocean concentrate and maintenance The gel mixture can maintain a stable size in a short period of time, and the process of deep-sea concentrate and maintenance of gel microcellular particles has been completed in the 0th second after mixing, and the particle size will not change significantly within a short period of time (5 minutes). Changed to indicate that this real-time deep ocean concentrate mixed with maintenance gel is suitable for immediate application to human skin after mixing.

Table 6. Dynamic laser particle size analysis at different time points after real-time mixing of deep ocean concentrate and maintenance gel

From the above optical microscope and laser particle size experiments, it can be concluded that the effect of the micelles has been completed after the real-time mixing of the deep ocean concentrate and the maintenance gel, and the average particle size of the micelles is stable within 5 minutes after mixing. It means that the size of the particles will not be changed by the increase of time in a short period of time after the deep ocean concentrate and the maintenance gel are evenly mixed, and they are suitable for application to human skin immediately after mixing.

In addition, under a 200-fold optical microscope, observe the single-agent maintenance gel (as shown in Figure 6B), real-time mixing of pure water and maintenance gel (pure water: maintenance gel weight = 1:1) (as shown in Figure 6C) and Real-time mixing of deep-ocean concentrate and maintenance gel (deep-ocean concentrate weight: maintenance gel weight = 1:1) (Figure 6D) differences in micelle particles.

It can be seen from the optical microscope observation that the single-agent maintenance gel (as shown in Figure 6B), the real-time mixing of pure water and maintenance gel (pure water: maintenance gel weight = 1:1) (as shown in Figure 6C), the cream of the maintenance gel It cannot be uniformly dispersed, and the effect of particle microcells is effectively generated, so there is obvious aggregation phenomenon. On the contrary, real-time mixing of deep ocean concentrate and maintenance gel (weight of deep ocean concentrate: weight of maintenance gel = 1:1) (as shown in Figure 6D), it can be observed that the cream texture of maintenance gelatin immediately undergoes phase transfer (PhaseTransfer) and Particles with micelles formed with an average particle size of about 5.03 μm. Compared with general skin care products, the particle size of molecular clusters is 15-50 μm, which can be reduced by one-third to one-tenth, which helps to improve the probability of skin care product molecular clusters entering the deep layers of the skin more effectively.

Example 8. Driving Force Measurements for Real Time Mixing of Deep Ocean Concentrate and Maintenance Gel

This experiment was commissioned by the Institute of Materials and Chemical Engineering of the Industrial Technology Research Institute to carry out the experiment, and the conductivity analyzer was used to measure the real-time mixing of deep-sea concentrate and maintenance gel (weight of deep-sea concentrate: weight of maintenance gel = 1:1). Conductivity, which in turn explains the propulsion of this real-time blend of Deep Ocean Concentrate and Maintenance Gel.

The conductivity of the mixture was measured using a conductivity analyzer, which were the control group (a) pure water and the experimental group (b) real-time mixing of pure water and maintenance gel (pure water: maintenance gel weight = 1:1) and (c) ) Real-time mixing of deep-sea concentrated solution and maintenance gel (weight of deep-sea concentrated solution: weight of maintenance gel = 1:1), after real-time mixing of samples, the analyzer is directly used for measurement.

Table 7. Conductivity tests of deep ocean concentrates and different mixtures

From the conductivity test, it is known that the conductivity of the experimental group (c) real-time mixing of deep-ocean concentrate and maintenance gel (weight of deep-ocean concentrate: maintenance gel weight = 1:1) exceeds that of the experimental group (b) real-time mixing pure The conductivity of water and maintenance gel (pure water: maintenance gel weight = 1:1) is more than 20 times. Even up to 12,250 times higher than the control group (a) pure water, this significant difference in conductivity shows that real-time mixing of deep ocean concentrate and maintenance gel (deep ocean concentrate weight: maintenance gel weight = 1:1) provides The good driving force of the mixture, through this driving force, promotes the effective absorption of skin care products into the deep layers of the skin by the human body, and improves the efficacy of skin care and maintenance formulas.

All features disclosed in the invention should be implemented in any combination. Each feature disclosed herein may be replaced by a substitute of the same, equivalent or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is only one embodiment of a class of equivalent or similar features.

Claims (9)

1. A deep-ocean concentrate skin care and maintenance set, characterized in that it comprises a first dose and a second dose, wherein the first dose is a deep-ocean concentrate, and the second dose is a skin care and maintenance formula; Wherein, after the deep-sea concentrate is mixed with the skin care formula, the effective concentration of the deep-sea concentrate is 1.25-50 v/v%; the hardness of the deep-sea concentrate is 80,000-420,000 mg/l, The salinity is 380～430‰; the magnesium concentration of the deep ocean concentrate is 6,500～110,000mg/l; the sodium concentration is 3,800～30,000mg/l, the potassium concentration is 400～18,000mg/l, The calcium concentration is 100-500 mg/l; the skin care and maintenance formula is in the form of gel or cream. 2 . The deep-sea concentrate skin care and maintenance kit according to claim 1 , wherein the deep-sea concentrate contains 1000-1500 mg of calcium, 1400-2100 mg of magnesium, and 2000-4000 mg of sodium per 100 g. 3 . , 1400-2100mg of potassium, sulfate <6.5wt%, and a solution of marine minerals with a specific gravity of 1.17-1.32g/ ^cm3 . 3. The deep-sea concentrate skin care and maintenance kit of claim 1, wherein the deep-sea concentrate is obtained from a water source below 200 m in the ocean. 4. The deep-sea concentrate skin care and maintenance kit of claim 1, wherein the deep-sea concentrate is obtained from a water source below 500 m in the ocean. 5. The deep-ocean concentrate skin care and maintenance kit of claim 1, wherein the skin care and maintenance formula is a whitening product, a moisturizing product, a wrinkle-removing product, a wound-healing product, a medical or cosmetic introduction product one or more of. 6. The deep-sea concentrate skin care and maintenance kit of claim 5, wherein the anti-wrinkle product is a collagen-promoting product. 7. The deep-sea concentrate skin care and maintenance kit of claim 1, wherein the deep-sea concentrate is liquid or powder. 8. The application of a deep ocean concentrate skin care kit for preparing a pharmaceutical composition for treating or maintaining skin, comprising administering the deep ocean concentrate skin care kit as claimed in claim 1 immediately after the preparation is completed onto the skin. 9 . The application according to claim 8 , wherein the deep ocean concentrate skin care and maintenance kit can quickly enter the skin, reach the dermis layer, and achieve wrinkle removal, whitening and firming. 10 .

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