CN1113103C - CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium - Google Patents
CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium Download PDFInfo
- Publication number
- CN1113103C CN1113103C CN96119034A CN96119034A CN1113103C CN 1113103 C CN1113103 C CN 1113103C CN 96119034 A CN96119034 A CN 96119034A CN 96119034 A CN96119034 A CN 96119034A CN 1113103 C CN1113103 C CN 1113103C
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- Prior art keywords
- indicator
- absorption
- solution
- concentration
- indication
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 238000010521 absorption reaction Methods 0.000 title claims abstract description 62
- 239000003153 chemical reaction reagent Substances 0.000 title abstract description 8
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 23
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000009007 Diagnostic Kit Methods 0.000 claims abstract description 13
- 239000004202 carbamide Substances 0.000 claims abstract description 11
- 241000590002 Helicobacter pylori Species 0.000 claims abstract description 10
- 229940037467 helicobacter pylori Drugs 0.000 claims abstract description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 51
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 16
- KGWDUNBJIMUFAP-KVVVOXFISA-N Ethanolamine Oleate Chemical compound NCCO.CCCCCCCC\C=C/CCCCCCCC(O)=O KGWDUNBJIMUFAP-KVVVOXFISA-N 0.000 claims description 15
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 9
- XSQUKJJJFZCRTK-NJFSPNSNSA-N UREA C 14 Chemical compound N[14C](N)=O XSQUKJJJFZCRTK-NJFSPNSNSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 6
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 6
- 238000003745 diagnosis Methods 0.000 claims description 6
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 claims description 4
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 3
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 2
- 239000012141 concentrate Substances 0.000 claims 1
- 229920006395 saturated elastomer Polymers 0.000 abstract description 11
- 238000001514 detection method Methods 0.000 abstract description 3
- 230000005855 radiation Effects 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 34
- 238000000034 method Methods 0.000 description 19
- UREZNYTWGJKWBI-UHFFFAOYSA-M benzethonium chloride Chemical compound [Cl-].C1=CC(C(C)(C)CC(C)(C)C)=CC=C1OCCOCC[N+](C)(C)CC1=CC=CC=C1 UREZNYTWGJKWBI-UHFFFAOYSA-M 0.000 description 11
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
- LDKDGDIWEUUXSH-UHFFFAOYSA-N Thymophthalein Chemical compound C1=C(O)C(C(C)C)=CC(C2(C3=CC=CC=C3C(=O)O2)C=2C(=CC(O)=C(C(C)C)C=2)C)=C1C LDKDGDIWEUUXSH-UHFFFAOYSA-N 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- 230000007704 transition Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 231100000987 absorbed dose Toxicity 0.000 description 2
- 239000006096 absorbing agent Substances 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- SIYLLGKDQZGJHK-UHFFFAOYSA-N dimethyl-(phenylmethyl)-[2-[2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethyl]ammonium Chemical compound C1=CC(C(C)(C)CC(C)(C)C)=CC=C1OCCOCC[N+](C)(C)CC1=CC=CC=C1 SIYLLGKDQZGJHK-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- CREICILGVGNQBH-UHFFFAOYSA-N phenolphthalol Chemical compound OCC1=CC=CC=C1C(C=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 CREICILGVGNQBH-UHFFFAOYSA-N 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229950000184 urea c 14 Drugs 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to a CO2 absorption indicator and <14>C-UBT helicobacter pylori (hp) diagnostic kit using the CO2 absorption indicator. The CO2 absorption indicator is composed of an absorption agent ethanolamine solution and an indicator super-high concentration phenolphthalein indicator, wherein the latter comprises the <14> C-Urea whose detection dosage is below luCi in each case and the CO2 absorption indicator, namely that an end point for absorbing CO2 in a saturated way by the indicator of super-high concentration is matched with a color change point of the indicator so that unaided eyes can judge the absorption amount of the CO2 by color change easily, and accordingly, the reagent cost and radiation dose can be greatly reduced; the toxicity and the corrosiveness are reduced.
Description
The present invention relates to applied chemistry, be specifically related to medicochemistry reagent, more particularly, relate to a kind of CO that liquid dodges sample preparation and can judge absorbed dose with appearance method that can be used for
2Absorption Indication and utilize the ultra micro dosage of this Absorption Indication
14The helicobacter pylori of C-UBT (Hp) diagnostic kit.
In dodging liquid medical science and biologic applications technology, a certain amount of CO that needs the quantitative collection human body to breathe out sometimes
2, dodge the conditions such as accessibility of measuring and gathering in order to satisfy liquid, require used absorption agent to have following characteristic: 1) a certain amount of absorption agent can absorb a certain amount of CO
2, and under physical environment, the product after absorbed dose and the absorption keeps stable; 2) available appearance method is judged finishing of absorption process; The color of absorption agent was colourless when 3) absorption was finished.4) low chemoluminescence.The existing carbon-dioxide absorbent that can meet above-mentioned four requirements that uses in the world is normally with the hyamine 10-X methanol solution of thymolphthalein as indicator, and very expensive and the poisonous and corrodibility of hyamine 10-X is very unfavorable to its promotion and application.
On the other hand, detect helicobacter pylori (Helicobacter pylori is called for short Hp down) and align and make a definite diagnosis the disconnected digestive tract diseases cause of disease, significant clinically to correct treatment, radical curing of disease.In the method for existing six kinds of detection Hp, no pain, non-invasive method have two kinds: the breathalyse method of serology immune analysis method and labeled urea.In the breathalyse method,
14C-Urea Breath Test (claims with doffing
14C-UBT) be to reflect in real time that Hp infects the sensitive and special method of height of present situation.Even to this day,
14The major cause that the C-UBT method is not extensively promoted the use of is that the used reagent cost of this method is too high, two kinds of main agents
14C urea
14C-Urea) and hyamine 10-X (Hyamineor Benzethonium hydroxide) cost an arm and a leg.
One object of the present invention is to provide a kind of novel CO
2Absorption Indication, this CO
2Absorption Indication is suitable for liquid and dodges sample preparation and can judge CO with appearance method
2Absorbed dose can overcome the shortcoming of used hyamine 10-X absorption agent, and cost is low, nontoxicity and corrodibility.
Another object of the present invention is to provide a kind of CO of the present invention that utilizes
2Novel helicobacter pylori (Hp) diagnostic kit of Absorption Indication structure, this test kit can overcome the shortcoming of prior art, have extremely low cost, and good security and ease of use are arranged, thereby make it generally to promote the use of in developing country and economically underdeveloped area.
Above-mentioned purpose of the present invention realizes by following technological approaches: 1). will detect in the agents useful for same at every turn
14The consumption of C-Urea is reduced to less than 1uCi from the minimum amount 1uCi that had reported, even can only use the dosage of 0.5uCi; 2). detecting Hp's
14In the C-UBT method, replace the conventional hyamine 10-X (Hyamine or Benzethonium hydroxide) that uses with thanomin (Ethanolamine); 3) use the ultrahigh concentration indicator, solve thanomin saturated absorption CO
2Terminal point can not be with meeting the problem that liquid dodges the indicator indication that sample preparation requires.Though thanomin can absorb CO
2Be well-known already, still, why exist
14Use expensive hyamine 10-X among the C-UBT, reason is always,
14In the C-UBT test, require absorption agent can not only absorb CO
2, and the terminal point of saturated absorption must with the naked eye can differentiate its color easily and indicate from coloured indicator that changes to colourless (liquid dodge measurement requirement terminal point be colourless), and the color transition point of indicator must match with the terminal point of absorption agent saturated absorption.In the prior art, mix thymolphthalein (Thymolphthalein) indicator (blue look changes to colourless) with hyamine 10-X and can satisfy above-mentioned requirements; For thanomin,, then do not find the indicator that can satisfy above-mentioned requirements after being used with it as yet if concentration is routinely used indicator.Phenolphthalein indicator is slight redness in the thanomin methanol solution, but concentration is used indicator routinely, it is the phenolphthalein indicator that every 50ml solution adds about 3 0.1-0.2% concentration alcoholic solutions, every about 50ul, the colour-change that is produced is very slight, be difficult for the naked eye differentiating what is more important, the color transition point of indicator and thanomin saturated absorption CO
2The terminal point difference, promptly the pH value of color transition point is far above the pH value of the terminal point of saturated absorption, therefore do not indicate the terminal point of saturated absorption; Phenolphthalein indicator is scarlet in the thanomin aqueous solution, but absorbs CO
2Still colour-fast during saturated terminal point, can not meet the demands.The phenomenon that the present invention utilizes the indicator of the same colour of higher concentration that color transition point is moved to low pH direction, tens of times to hundreds of times the egmol solution that with ultrahigh concentration is typical concentrations coincide the indicator discoloration point and the terminal point of saturated absorption as indicator, make change in color more obvious simultaneously, be easy to observe.Like this, do not absorb CO
2The thanomin methanol solution under this super dense indicator effect, be scarlet, and to saturated absorption CO
2Terminal point can take off again for colourless, the required time of variable color process is suitable for medical diagnosis and uses.Satisfied the terminal point that with the naked eye to see saturated absorption, can meet liquid again and dodge the colourless and low chemiluminescent requirement of measurement requirement by change in color.So just solve and detect Hp's
14The expensive problem that must use hyamine 10-X in the C-UBT method and cause.
For realizing first purpose of the present invention, construct a kind of CO
2Absorption Indication is made up of absorption agent and indicator, it is characterized in that described absorption agent is ethanolamine solutions or diethanolamine solution or triethylamine or ethylenediamine solution, and solvent can be anhydrous methanol, dehydrated alcohol or ethylene glycol.Described indicator is the ultrahigh concentration phenolphthalein indicator, and the concentration of described ultrahigh concentration phenolphthalein indicator in described Absorption Indication is (0.005-0.2) % (g/ml).
The CO that provides according to first aspect present invention
2Absorption Indication, the solvent that it is characterized in that described ethanolamine solutions or diethanolamine solution or triethylamine solution or ethylenediamine solution can be anhydrous methanol, dehydrated alcohol or ethylene glycol, concentration can be 1-4mol.
For realizing second purpose of the present invention, construct a kind of CO of the present invention that utilizes
2Absorption Indication carries out
14C-UBT helicobacter pylori (Hp) diagnosis kits, it is characterized in that: described test kit comprises: every example detects consumption and is lower than 1uCi's
14C-Urea and the CO that forms by absorption agent ethanolamine solutions and indicator ultrahigh concentration phenolphthalein indicator
2Absorption Indication, the concentration of described ultrahigh concentration phenolphthalein indicator in described Absorption Indication is (0.005-0.2) % (g/ml).
Hp diagnostic kit according to second aspect present invention provides is characterized in that, every example detects used
14The C-Urea consumption can be 0.5uCi.
Hp diagnostic kit according to second aspect present invention provides is characterized in that, the concentration of described ultrahigh concentration phenolphthalein indicator in described Absorption Indication is 0.08% (g/ml) preferably.
According to the Hp diagnostic kit that second aspect present invention provides, the solvent that it is characterized in that ethanolamine solutions can be anhydrous methanol, dehydrated alcohol or ethylene glycol, and concentration can be 1-4mol.
Hp diagnostic kit according to second aspect present invention provides is characterized in that, the described CO that is made up of thanomin methanol solution and ultrahigh concentration phenolphthalein indicator
2In Absorption Indication is divided in a plurality of (2 integral multiples) the liquid flashing counting bottle, described test kit also comprises the container that concentrated scintillation solution (genus prior art) is housed, as long as in this concentrated scintillation solution, add its volume 2.5-3 doubly dimethylbenzene or toluene, can use, described test kit also comprises and being equipped with
14The container of C-Urea.
Implement CO provided by the invention
2Absorption Indication is than traditional CO
2Absorption agent has that cost reduces greatly and the free of contamination advantage of nontoxicity, and utilizes above-mentioned CO according to of the invention process
2The helicobacter pylori diagnostic reagent kit of Absorption Indication structure is with existing survey Hp's
14The C-UBT method is compared, and has following advantage: reagent cost is reduced to original below 1/3, and the suffered radiation dose of patient reduces, and thanomin is compared with the hyamine 10-X methanol solution and do not had toxicity and corrodibility, the operation of being more convenient for.
In conjunction with optimum implementation of the present invention, further specify characteristics of the present invention:
According to CO provided by the invention
2Absorption Indication (the thanomin alcoholic solution under the cooperation of ultrahigh concentration phenolphthalein indicator) is equally applicable to the CO as other breathalyse (Breath Test) method
2Absorption agent.
According to provided by the invention
14C-UBT helicobacter pylori (Hp) diagnostic kit is that the thanomin methanol solution under cooperating with the ultrahigh concentration phenolphthalein indicator replaces hyamine 10-X methanol solution (under the thymolphthalein indicator cooperates) as diagnosis Hp's
14CO in the C-UBT method
2Absorption agent, and every example
14The C-Urea consumption can be lower than 1uCi, even the used 14C-Urea consumption of every example detection can be 0.5uCi.Wherein the concentration of indicator in solution is (0.005-0.2) % (g/ml), the best is 0.08%, this concentration is that tens of times to hundreds of times of conventional concentration are (common, the phenolphthalein indicator 3-4 that splashes into 0.1% (g/ml) concentration in every 50ml solution drips, every about 50ul), solvent can be the alcohols amphoteric solvent.Phenolphthalein dry powder directly can be dropped in the absorbent solution according to the above ratio, make it dissolving (is solvent with the methyl alcohol in the absorbent solution).The solvent of described ethanolamine solutions can be that anhydrous methanol also can be dehydrated alcohol or ethylene glycol, and its concentration can be 1-4mol.
In Hp diagnostic kit provided by the invention, the thanomin methanol solution has been sub-divided in the liquid flashing counting bottle.Concentrated scintillation solution is equipped with in the box, if in this concentrated scintillation solution, add its volume 2.5-3 doubly dimethylbenzene or toluene, can use.
Embodiment one
1. reagent dosage:
(1)
14C-Urea 1uCi;
(2) absorption agent and indicator: absorption agent is that the absorption agent that 0.08% (g/ml) will make like this is called standard absorber with the thanomin absolute methanol solution of 2M concentration with the concentration that makes it in solution in the phenolphthalein dry powder adding absorbent solution.
2. using method:
Step 1: with 1uCi's
14C-Urea is dissolved in about 25ml distilled water standby, and is ready to two bottles of liquid sudden strain of a muscle bottles (screwing lid) that the 2ml standard absorber respectively is housed;
Step 2: after gargling on an empty stomach early morning, exhale to the absorption agent that a liquid dodges in the bottle by flexible pipe, liquid becomes colourlessly from redness to the bottle, tightens lid, as the background bottle;
Step 3: drink flatly and be dissolved with 1uCi's
14The distilled water of C-Urea is gargled with one glass of distilled water more in addition;
Step 4: sat quietly 20 minutes;
Step 5: blow to fading fully to the absorption agent that another liquid dodges in the bottle, tighten bottle cap;
Step 6: dodge the concentrated scintillation solution that this test kit after bottle adds the 4.5ml dilution is equipped with to every liquid, and go up liquid scintillation instrument and measure;
Step 7: if measuring result is more than or equal to 250dpm/mmol CO
2, then be diagnosed as the positive.
Embodiment two:
1. reagent dosage: except
14The C-Urea consumption is outside the 0.5uCi, and all the other are with scheme one.
2. step: except the time of sitting quietly changes 10 minutes into, positive threshold value is 100dpm/mmolCO
2Outward, all the other are with embodiment one.
Claims (7)
1. CO
2Absorption Indication, form by absorption agent and indicator, described absorption agent is ethanolamine solutions or diethanolamine solution or triethanolamine solution or ethylenediamine solution, described indicator is a phenolphthalein indicator, it is characterized in that: the concentration of described phenolphthalein indicator in described Absorption Indication is 0.005-0.2% (g/ml).
2. CO according to claim 1
2Absorption Indication is characterized in that, the solvent of described ethanolamine solutions or diethanolamine solution or triethanolamine solution is anhydrous methanol, dehydrated alcohol or ethylene glycol, and concentration is 1-4mol.
3. one kind is used to carry out
14C-UBT helicobacter pylori (Hp) diagnosis kits is characterized in that described test kit comprises: every example detects consumption and is lower than 1uCi's
14C-Urea, and the CO that forms by absorption agent ethanolamine solutions and indicator phenolphthalein indicator
2Absorption Indication, the concentration of described phenolphthalein indicator in described Absorption Indication are 0.005-0.2% (g/ml).
4. according to the described diagnostic kit of claim 3, it is characterized in that every example detects used
14The C-Urea consumption is 0.5uCi.
5. according to the described diagnostic kit of claim 3, it is characterized in that the concentration of described phenolphthalein indicator in described Absorption Indication is 0.08% (g/ml).
6. according to the described diagnostic kit of claim 3, it is characterized in that the solvent of described ethanolamine solutions can be an anhydrous methanol, also can be dehydrated alcohol or ethylene glycol, and concentration can be 1-4mol.
7. according to the described diagnostic kit of claim 3, it is characterized in that the described CO that forms by thanomin methanol solution and phenolphthalein indicator
2Absorption Indication is divided in a plurality of liquid flashing counting bottles, and described test kit comprises and being equipped with
14The container of C-Urea and the container that concentrates scintillation solution is housed, if in concentrated scintillation solution wherein, add its volume 2.5-3 doubly dimethylbenzene or toluene can use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119034A CN1113103C (en) | 1996-02-14 | 1996-02-14 | CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119034A CN1113103C (en) | 1996-02-14 | 1996-02-14 | CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1157258A CN1157258A (en) | 1997-08-20 |
| CN1113103C true CN1113103C (en) | 2003-07-02 |
Family
ID=5125496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96119034A Expired - Lifetime CN1113103C (en) | 1996-02-14 | 1996-02-14 | CO2 absorption indicator and reagent kit for diagnosing pyloric Helicobacterium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1113103C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926240A (en) * | 2013-09-30 | 2014-07-16 | 安徽养和医疗器械设备有限公司 | Carbon dioxide absorption indicator |
| KR101840773B1 (en) | 2013-11-26 | 2018-03-21 | 차이나 페트로리움 앤드 케미컬 코포레이션 | Beta molecular sieve, preparation method therefor and hydrogenation catalyst containing same |
| CN107229065A (en) * | 2017-05-10 | 2017-10-03 | 江苏万略医药科技有限公司 | Scintillation solution |
| CN116899392A (en) * | 2023-07-18 | 2023-10-20 | 天津大学 | Volatile fluorine anesthetic absorption liquid and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87105619A (en) * | 1986-08-13 | 1988-02-24 | 卡尔·格·费德尔 | indicator device |
| CN87207896U (en) * | 1987-05-06 | 1988-03-02 | 广州中山医科大学第一附属医院 | Soaking slice fast bacteria-culturing box |
| CN1093409A (en) * | 1993-03-06 | 1994-10-12 | 宋家益 | Coliform quick test test paper and production method thereof |
| CN1114748A (en) * | 1994-12-03 | 1996-01-10 | 中国人民解放军第四七八医院 | Carbon dioxide indicator and preparation process |
-
1996
- 1996-02-14 CN CN96119034A patent/CN1113103C/en not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87105619A (en) * | 1986-08-13 | 1988-02-24 | 卡尔·格·费德尔 | indicator device |
| CN87207896U (en) * | 1987-05-06 | 1988-03-02 | 广州中山医科大学第一附属医院 | Soaking slice fast bacteria-culturing box |
| CN1093409A (en) * | 1993-03-06 | 1994-10-12 | 宋家益 | Coliform quick test test paper and production method thereof |
| CN1114748A (en) * | 1994-12-03 | 1996-01-10 | 中国人民解放军第四七八医院 | Carbon dioxide indicator and preparation process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1157258A (en) | 1997-08-20 |
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Owner name: SHENZHEN CITY ZHONGHE HAIDEWEI BIOLOGY TECHNOLOGY Free format text: FORMER OWNER: MA YONGJIAN Effective date: 20061110 |
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Effective date of registration: 20061110 Address after: 6 building, R1-B building, South District, Shenzhen hi tech Industrial Park, 518000 Patentee after: Shenzhen Zhonghe Headway Bio Sci&Tech Co., Ltd. Address before: 518026 room 12, 601 deep and large village, Shenzhen, Guangdong, Futian District Patentee before: Ma Yongjian |
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