CN1114613C - 蛋白质溶液的铝/铁处理和膜浓缩方法 - Google Patents

蛋白质溶液的铝/铁处理和膜浓缩方法 Download PDF

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Publication number
CN1114613C
CN1114613C CN96194380.7A CN96194380A CN1114613C CN 1114613 C CN1114613 C CN 1114613C CN 96194380 A CN96194380 A CN 96194380A CN 1114613 C CN1114613 C CN 1114613C
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China
Prior art keywords
protein solution
test
aluminum
concentration
protein
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Expired - Fee Related
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CN96194380.7A
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English (en)
Chinese (zh)
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CN1186498A (zh
Inventor
T·K·尼尔森
M·A·拉斯特森
N－V·尼尔森
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Novozymes AS
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Novozymes AS
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN96194380.7A 1995-06-02 1996-05-31 蛋白质溶液的铝/铁处理和膜浓缩方法 Expired - Fee Related CN1114613C (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK0626/1995 1995-06-02
DK62695 1995-06-02
DK0626/95 1995-06-02

Publications (2)

Publication Number Publication Date
CN1186498A CN1186498A (zh) 1998-07-01
CN1114613C true CN1114613C (zh) 2003-07-16

Family

ID=8095720

Family Applications (1)

Application Number Title Priority Date Filing Date
CN96194380.7A Expired - Fee Related CN1114613C (zh) 1995-06-02 1996-05-31 蛋白质溶液的铝/铁处理和膜浓缩方法

Country Status (9)

Country Link
EP (1) EP0828753B1 (2)
JP (1) JPH11511118A (2)
CN (1) CN1114613C (2)
AT (1) ATE235506T1 (2)
AU (1) AU5996996A (2)
DE (1) DE69626976T2 (2)
DK (1) DK0828753T3 (2)
IN (1) IN181859B (2)
WO (1) WO1996038469A1 (2)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2810667B1 (fr) * 2000-06-23 2004-09-03 Warner Lambert Co Procede d'isolement et de purification d'une proteine, et proteine obtenue
DK2169076T3 (en) 2001-02-21 2017-02-06 Basf Enzymes Llc Enzymes with alpha-amylase activity and methods for their use
US7659102B2 (en) 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
WO2002092802A1 (en) * 2001-05-14 2002-11-21 Diversa Corporation Novel methods of enzyme purification
US20030119734A1 (en) 2001-06-28 2003-06-26 Flink James M. Stable formulation of modified GLP-1
US7118891B2 (en) 2001-12-11 2006-10-10 Novozymes A/S Crystal harvest from fermentation broth
AU2002349300A1 (en) * 2001-12-11 2003-06-23 Novozymes A/S Process for harvesting crystalline particles from fermentation broth
CN111484996A (zh) 2003-03-06 2020-08-04 巴斯夫酶有限责任公司 淀粉酶、编码它们的核酸及其制备和应用方法
JP4936884B2 (ja) 2003-06-03 2012-05-23 ノボ・ノルデイスク・エー／エス 安定化された薬学的ペプチド組成物
WO2004105790A1 (en) 2003-06-03 2004-12-09 Novo Nordisk A/S Stabilized pharmaceutical peptide compositions
PT1687019T (pt) 2003-11-20 2018-02-26 Novo Nordisk As Formulações de péptidos que contêm polietileno glicol que são ótimas para produção e para utilização em dispositivos de injeção
EP1789434B1 (en) 2004-08-31 2013-11-20 Novo Nordisk A/S Use of tris(hydroxymethyl) aminomethane for the stabilization of peptides, polypeptides and proteins
EP2494983B1 (en) 2004-11-12 2019-04-24 Novo Nordisk A/S Stable formulations of glp-1
WO2007035283A1 (en) * 2005-09-15 2007-03-29 Wyeth Protein floculation using salts
BRPI0719449A2 (pt) 2006-12-21 2017-06-20 Syngenta Participations Ag molécula de ácido nucléico isolada, sintética ou recombinante, polipeptídeo isolado, planta ou parte de planta, eículo de clonagem que compreende um ácido nucléico, cassete de expressão que compreende uma molécula de ácido nucléico e método para hidrolisar um polissacarídeo, oligossacarídeo ou amido
US20120252066A1 (en) * 2011-03-29 2012-10-04 Heng Meng H Methods of foam control
US20150291656A1 (en) 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
WO2015075302A1 (en) 2013-11-19 2015-05-28 Kemira Oyj Method for reducing colour of a biotechnological liquid
HUE066356T2 (hu) 2017-08-24 2024-07-28 Novo Nordisk As GLP-1 készítmények és alkalmazásaik
CN108949703A (zh) * 2018-06-28 2018-12-07 大连理工大学 一种半纤维小体类似物粗提纯方法
EP3983425B1 (en) 2019-06-13 2025-10-01 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
PE20221575A1 (es) 2020-02-18 2022-10-06 Novo Nordisk As Formulaciones farmaceuticas
US20230331775A1 (en) 2020-09-22 2023-10-19 Basf Se Method for recovering a protein from a fermentation broth comprising a high degree of lysed cells
CN112500471B (zh) * 2020-12-22 2022-09-27 中美华世通生物医药科技(武汉)股份有限公司 一种制备高澄清度蛋白琥珀酸铁的方法
US20260062690A1 (en) 2022-08-02 2026-03-05 Basf Se Stabilized protein production process using bacillus host cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3795586A (en) * 1969-08-11 1974-03-05 Pabst Brewing Co Recovery of enzymes
IL85255A0 (en) * 1987-01-30 1988-07-31 Int Minerals & Chem Corp Recovery of somatotropin from dilute aqueous solutions
US4894444A (en) * 1987-07-16 1990-01-16 Merck & Co., Inc. Recovery and purification of immunogens from immunogen-alum complexes
DE3915277A1 (de) * 1989-05-10 1990-11-15 Henkel Kgaa Verfahren zur reinigung von enzymfluessigkonzentraten
DE4037530A1 (de) * 1990-11-26 1992-05-27 Henkel Kgaa Faellungsverfahren fuer exocellulaere proteine
DK91092D0 (2) * 1992-07-10 1992-07-10 Novo Nordisk As

Also Published As

Publication number Publication date
ATE235506T1 (de) 2003-04-15
WO1996038469A1 (en) 1996-12-05
IN181859B (2) 1998-10-03
DK0828753T3 (da) 2003-06-30
EP0828753A1 (en) 1998-03-18
JPH11511118A (ja) 1999-09-28
AU5996996A (en) 1996-12-18
DE69626976T2 (de) 2004-03-04
EP0828753B1 (en) 2003-03-26
CN1186498A (zh) 1998-07-01
DE69626976D1 (de) 2003-04-30

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Applicant before: Novo Nordisk A/S

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Termination date: 20120531