CN112972748A - Method for preparing wound hemostatic dressing by adopting chitosan - Google Patents
Method for preparing wound hemostatic dressing by adopting chitosan Download PDFInfo
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- CN112972748A CN112972748A CN202110143671.3A CN202110143671A CN112972748A CN 112972748 A CN112972748 A CN 112972748A CN 202110143671 A CN202110143671 A CN 202110143671A CN 112972748 A CN112972748 A CN 112972748A
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 99
- 230000002439 hemostatic effect Effects 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 81
- 229920002678 cellulose Polymers 0.000 claims abstract description 59
- 239000001913 cellulose Substances 0.000 claims abstract description 59
- 230000023597 hemostasis Effects 0.000 claims abstract description 36
- 239000007787 solid Substances 0.000 claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 239000011521 glass Substances 0.000 claims abstract description 21
- 238000004108 freeze drying Methods 0.000 claims abstract description 14
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 11
- 239000002131 composite material Substances 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 239000004627 regenerated cellulose Substances 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims description 25
- 239000002608 ionic liquid Substances 0.000 claims description 17
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 8
- 239000012498 ultrapure water Substances 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000007711 solidification Methods 0.000 claims description 6
- 230000008023 solidification Effects 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 5
- AOBIOSPNXBMOAT-UHFFFAOYSA-N 2-[2-(oxiran-2-ylmethoxy)ethoxymethyl]oxirane Chemical compound C1OC1COCCOCC1CO1 AOBIOSPNXBMOAT-UHFFFAOYSA-N 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 229920001223 polyethylene glycol Polymers 0.000 claims description 4
- UWFRVQVNYNPBEF-UHFFFAOYSA-N 1-(2,4-dimethylphenyl)propan-1-one Chemical compound CCC(=O)C1=CC=C(C)C=C1C UWFRVQVNYNPBEF-UHFFFAOYSA-N 0.000 claims description 3
- SHKUUQIDMUMQQK-UHFFFAOYSA-N 2-[4-(oxiran-2-ylmethoxy)butoxymethyl]oxirane Chemical compound C1OC1COCCCCOCC1CO1 SHKUUQIDMUMQQK-UHFFFAOYSA-N 0.000 claims description 3
- 239000008280 blood Substances 0.000 abstract description 7
- 210000004369 blood Anatomy 0.000 abstract description 7
- 230000006378 damage Effects 0.000 abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 3
- 239000012567 medical material Substances 0.000 abstract description 2
- 208000027418 Wounds and injury Diseases 0.000 description 33
- 206010052428 Wound Diseases 0.000 description 32
- 230000006196 deacetylation Effects 0.000 description 4
- 238000003381 deacetylation reaction Methods 0.000 description 4
- 208000014674 injury Diseases 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 230000000740 bleeding effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000002861 polymer material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000008733 trauma Effects 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229940030225 antihemorrhagics Drugs 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- -1 cationic polysaccharide Chemical class 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000002874 hemostatic agent Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- General Health & Medical Sciences (AREA)
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- Dispersion Chemistry (AREA)
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Abstract
The invention belongs to the technical field of medical materials, and discloses a method for preparing a wound hemostatic dressing by using chitosan, which comprises the following steps: (1) preparing a chitosan hemostatic sponge: preparing chitosan powder into a chitosan acetic acid solution, adding a cross-linking agent, and freeze-drying to obtain a chitosan hemostatic sponge; (2) preparation of regenerated cellulose membrane: preparing the dried and activated cellulose into a cellulose solution, pouring the prepared cellulose solution on a glass plate, and moving a wire rod to obtain a transparent solid cellulose membrane; (3) preparing the composite wound hemostatic dressing: and adhering the chitosan hemostatic sponge on the solid cellulose membrane to obtain the wound hemostatic dressing. The chitosan wound hemostasis dressing prepared by the invention has good antibacterial performance and hemostasis effect, the chitosan hemostasis sponge has a loose structure, is easy to absorb blood to form gel, is easy to fall off, has good comfort, and the chitosan wound hemostasis dressing with the solid cellulose membrane protective shell is not easy to damage.
Description
Technical Field
The invention belongs to the technical field of medical materials, and particularly relates to a method for preparing a wound hemostatic dressing by using chitosan.
Background
Effective hemostasis is a main problem to be solved urgently in surgical operations and trauma, the basic methods include three methods of heating hemostasis, mechanical hemostasis and chemical hemostasis, and local hemostasis medicine is one of chemical hemostasis methods.
With the development and research of biological materials, medical natural polymer materials such as cellulose, gelatin, chitin and the like and medical synthetic high polymer materials such as polyvinyl alcohol, collagen and the like are used in hemostatic and trauma dressing, so that the development and research of local hemostatic enter a new stage. Since Malette et al reported chitosan as a hemostatic agent in 1983, scholars have tried to develop hemostatic dressings using chitosan. In 11 months 2002, a Hem Con bandage is approved by the American FDA, and clinical tests prove that the chitosan dressing can obviously reduce the amount of bleeding and the death rate compared with standard gauze dressings. The hemostatic effect of chitosan hemostatic dressing is also reported in China.
Chitosan is a unique cationic polysaccharide in the nature, has low allergenicity and natural antibacterial property, can quickly stop bleeding, and is particularly suitable for battlefield first aid; in addition, the chitosan has biocompatibility, can be degraded and absorbed in vivo, promotes wound healing, reduces scar generation, has an anti-inflammatory effect and the like, and therefore has great advantages in the application of hemostatic dressings, bacteriostatic dressings and wound repair dressings.
The prior chitosan hemostatic product is prepared by soaking gauze in chitosan solution and then drying, and the prepared chitosan dressing can cause secondary injury when being removed if being adhered to a wound during use. Therefore, the invention provides the chitosan hemostatic dressing without special odor.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the chitosan wound hemostasis dressing which avoids secondary damage caused by removal, and the prepared chitosan wound hemostasis dressing has good comfort level.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a method for preparing a wound hemostasis dressing by adopting chitosan comprises the following steps:
(1) preparing a chitosan hemostatic sponge: adding an acetic acid solution into chitosan powder, oscillating and dissolving at a constant temperature of 40 ℃ to obtain a chitosan acetic acid solution, adding a cross-linking agent into the chitosan acetic acid solution at room temperature, mechanically stirring uniformly, pouring into a culture dish, and freeze-drying in a freeze-drying machine to obtain the chitosan hemostatic sponge;
(2) preparation of regenerated cellulose membrane: putting the dried and activated cellulose into ionic liquid at room temperature, uniformly stirring, gradually raising the speed to 45 ℃, preserving heat for 1h, then slowly adding the ionic liquid, uniformly stirring, preserving heat for 2h at 45 ℃, slowly cooling the cellulose solution to 30 ℃, preheating a glass plate and a wire rod to 30 ℃, pouring the prepared cellulose solution on the glass plate, moving the wire rod, pre-solidifying the formed cellulose solution film at room temperature, and then transferring the glass plate to ultrapure water for solidification to obtain a transparent solid cellulose film;
(3) preparing the composite wound hemostatic dressing: and adhering the chitosan hemostatic sponge to the solid cellulose membrane to obtain the wound hemostatic dressing.
Further, the step (1) further comprises standing at room temperature for defoaming after the chitosan is completely shaken and dissolved.
Further, the acetic acid solution in the step (1) is an acetic acid solution with a concentration of 2%.
Further, the cross-linking agent is any one of 1, 4-butanediol diglycidyl ether, ethylene glycol diglycidyl ether and polyethylene glycol diglycidyl ether.
Furthermore, the addition amount of the cross-linking agent is 0.4-2.0% of the mass of the chitosan acetic acid solution.
Further, in the step (2), the cellulose is put into the ionic liquid at room temperature, the temperature is gradually increased to 45 ℃ according to the speed of 3 ℃/min, the ionic liquid is slowly added after the temperature is maintained for 1h, the ionic liquid is uniformly stirred and then the temperature is maintained at 45 ℃ for 2h, the stirring is carried out once every 20min during the temperature maintaining period, and the cellulose solution is slowly cooled to 30 ℃ at the speed of 0.5 ℃/min.
Further, in the step (2), the wire rod was moved at a rate of 1mm/s, and the formed cellulose liquid film was pre-coagulated at room temperature for 30min, and then the glass plate was transferred to ultra-pure water at 20 ℃ to be coagulated for 30min, to obtain a transparent solid cellulose film.
The invention has the beneficial effects that:
the invention provides a method for preparing a wound hemostasis dressing by adopting chitosan, which comprises the steps of firstly freeze-drying a chitosan acetic acid solution to obtain a chitosan hemostasis sponge, then preparing cellulose into a solid cellulose membrane, and finally adhering the chitosan hemostasis sponge on the solid cellulose membrane to obtain the chitosan wound hemostasis dressing. The prepared chitosan wound hemostasis dressing has good antibacterial performance and hemostasis effect, on one hand, the chitosan hemostasis sponge is loose in structure, the chitosan hemostasis sponge is easy to absorb blood to form gel and fall off, the chitosan hemostasis sponge is soft, when the blood of a wound is absorbed, the wound comfort is good, on the other hand, the solid cellulose membrane is high in tensile strength, the protective shell polysaccharide wound hemostasis dressing is not easy to damage, and the chitosan hemostasis sponge is prevented from being broken as soon as being pinched after the blood of the wound is absorbed.
Detailed Description
In order to make the objects, features and advantages of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the embodiments described below are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments that can be derived by one skilled in the art from the embodiments given herein are intended to be within the scope of the invention.
Example 1
A method for preparing a wound hemostasis dressing by adopting chitosan comprises the following steps:
(1) preparing a chitosan hemostatic sponge: adding 100ml of 2% acetic acid solution into 100g of chitosan powder (the viscosity is 100mPa & s, the deacetylation degree is more than or equal to 90%), oscillating and dissolving at the constant temperature of 40 ℃, standing and defoaming at room temperature after chitosan is completely oscillated and dissolved to obtain a chitosan acetic acid solution, adding 1ml of 1, 4-butanediol diglycidyl ether serving as a cross-linking agent into the chitosan acetic acid solution at room temperature, mechanically stirring uniformly, pouring into a culture dish with the diameter of 6.0cm, and freeze-drying in a freeze-drying machine to obtain chitosan hemostatic sponge with the diameter of 6 mm;
(2) preparation of regenerated cellulose membrane: placing 5.5g of dried and activated cellulose into 2.5ml of ionic liquid at room temperature, uniformly stirring, gradually heating to 45 ℃ at the speed of 3 ℃/min, preserving heat for 1h, then slowly adding 6ml of ionic liquid, preserving heat for 2h at 45 ℃ after uniformly stirring, stirring once every 20min during the heat preservation period, slowly cooling the cellulose solution to 30 ℃ at the speed of 0.5 ℃/min, preheating a glass plate and a wire rod to 30 ℃, pouring the prepared cellulose solution onto the glass plate, moving the wire rod at the speed of 1mm/s, pre-solidifying the formed cellulose liquid film for 30min at room temperature, and then transferring the glass plate into ultra-pure water at 20 ℃ for solidification for 30min to obtain a transparent solid cellulose film;
(3) preparing the composite wound hemostatic dressing: and adhering the obtained chitosan hemostatic sponge with the diameter of 6.0cm and the thickness of 6mm to the solid cellulose membrane to obtain the wound hemostatic dressing.
Example 2
A method for preparing a wound hemostasis dressing by adopting chitosan comprises the following steps:
(1) preparing a chitosan hemostatic sponge: adding 100ml of 2% acetic acid solution into 100g of chitosan powder (the viscosity is 100mPa & s, the deacetylation degree is more than or equal to 90%), oscillating and dissolving at the constant temperature of 40 ℃, standing and defoaming at room temperature after chitosan is completely oscillated and dissolved to obtain a chitosan acetic acid solution, adding 0.8ml of ethylene glycol diglycidyl ether serving as a cross-linking agent into the chitosan acetic acid solution at room temperature, mechanically stirring uniformly, pouring into a culture dish with the diameter of 6.0cm, and freeze-drying in a freeze-dryer to obtain 7mm chitosan hemostatic sponge;
(2) preparation of regenerated cellulose membrane: putting 5g of dried and activated cellulose into 5L of ionic liquid at room temperature, uniformly stirring, gradually heating to 45 ℃ at the speed of 3 ℃/min, preserving heat for 2ml, then slowly adding 5ml of ionic liquid, preserving heat for 2h at 45 ℃ after uniformly stirring, stirring once every 20min during the heat preservation period, slowly cooling the cellulose solution to 30 ℃ at the speed of 0.5 ℃/min, preheating a glass plate and a wire rod to 30 ℃, pouring the prepared cellulose solution onto the glass plate, moving the wire rod at the speed of 1mm/s, pre-solidifying a cellulose liquid film formed by the wire rod for 30min at room temperature, and then transferring the glass plate into 20 ℃ ultrapure water for solidification for 30min to obtain a transparent solid cellulose film;
(3) preparing the composite wound hemostatic dressing: and adhering the obtained chitosan hemostatic sponge with the diameter of 6.0cm and the thickness of 7mm to the solid cellulose membrane to obtain the wound hemostatic dressing.
Example 3
A method for preparing a wound hemostasis dressing by adopting chitosan comprises the following steps:
(1) preparing a chitosan hemostatic sponge: adding 200ml of 2% acetic acid solution into 150g of chitosan powder (the viscosity is 100mPa & s, the deacetylation degree is more than or equal to 90%), oscillating and dissolving at the constant temperature of 40 ℃, standing and defoaming at room temperature after chitosan is completely oscillated and dissolved to obtain chitosan acetic acid solution, adding 1.5ml of polyethylene glycol diglycidyl ether serving as a cross-linking agent into the chitosan acetic acid solution at room temperature, mechanically stirring uniformly, pouring into a culture dish with the diameter of 6.0cm, and freeze-drying in a freeze-drying machine to obtain chitosan hemostatic sponge after 8 mm;
(2) preparation of regenerated cellulose membrane: putting 5g of dried and activated cellulose into 2ml of ionic liquid at room temperature, uniformly stirring, gradually heating to 45 ℃ at the speed of 3 ℃/min, preserving heat for 1h, then slowly adding 6ml of ionic liquid, preserving heat for 2h at 45 ℃ after uniformly stirring, stirring once every 20min during the heat preservation period, slowly cooling the cellulose solution to 30 ℃ at the speed of 0.5 ℃/min, preheating a glass plate and a wire rod to 30 ℃, pouring the prepared cellulose solution onto the glass plate, moving the wire rod at the speed of 1mm/s, pre-solidifying a cellulose liquid film formed by the wire rod for 30min at room temperature, and then transferring the glass plate into 20 ℃ ultrapure water for solidification for 30min to obtain a transparent solid cellulose film;
(3) preparing the composite wound hemostatic dressing: and adhering the obtained chitosan hemostatic sponge with the diameter of 6.0cm and the thickness of 8mm to the solid cellulose membrane to obtain the wound hemostatic dressing.
Example 4
A method for preparing a wound hemostasis dressing by adopting chitosan comprises the following steps:
(1) preparing a chitosan hemostatic sponge: adding 120ml of 2% acetic acid solution into 160g of chitosan powder (the viscosity is 100mPa & s, the deacetylation degree is more than or equal to 90%), oscillating and dissolving at the constant temperature of 40 ℃, standing and defoaming at room temperature after chitosan is completely oscillated and dissolved to obtain a chitosan acetic acid solution, adding 1.2ml of polyethylene glycol diglycidyl ether serving as a cross-linking agent into the chitosan acetic acid solution at room temperature, mechanically stirring uniformly, pouring into a culture dish with the diameter of 6.0cm, and freeze-drying in a freeze-drying machine to obtain chitosan hemostatic sponge after 10 mm;
(2) preparation of regenerated cellulose membrane: putting 5g of dried and activated cellulose into 2.5ml of ionic liquid at room temperature, uniformly stirring, gradually heating to 45 ℃ at the speed of 3 ℃/min, preserving heat for 1h, then slowly adding 5ml of ionic liquid, preserving heat for 2h at 45 ℃ after uniformly stirring, stirring once every 20min during the heat preservation period, slowly cooling the cellulose solution to 30 ℃ at the speed of 0.5 ℃/min, preheating a glass plate and a wire rod to 30 ℃, pouring the prepared cellulose solution onto the glass plate, moving the wire rod at the speed of 1mm/s, pre-solidifying the formed cellulose liquid film for 30min at room temperature, and then transferring the glass plate into 20 ℃ ultrapure water for solidification for 30min to obtain a transparent solid cellulose film;
(3) preparing the composite wound hemostatic dressing: and adhering the obtained chitosan hemostatic sponge with the diameter of 6.0cm and the thickness of 10mm to the solid cellulose membrane to obtain the wound hemostatic dressing.
The chitosan hemostatic dressing is prepared by freeze-drying a chitosan acetic acid solution to obtain a chitosan hemostatic sponge, preparing cellulose into a solid cellulose membrane, and adhering the chitosan hemostatic sponge on the solid cellulose membrane. The prepared chitosan wound hemostasis dressing has good antibacterial performance and hemostasis effect, on one hand, the chitosan hemostasis sponge is loose in structure, the chitosan hemostasis sponge is easy to absorb blood to form gel and fall off, the chitosan hemostasis sponge is soft, when the blood of a wound is absorbed, the wound comfort is good, on the other hand, the solid cellulose membrane is high in tensile strength, the protective shell polysaccharide wound hemostasis dressing is not easy to damage, and the chitosan hemostasis sponge is prevented from being broken as soon as being pinched after the blood of the wound is absorbed.
Although the present invention has been described with reference to the specific embodiments, it should be understood that the scope of the present invention is not limited thereto, and those skilled in the art will appreciate that various modifications and variations can be made without inventive changes by those skilled in the art based on the technical solutions of the present invention.
Claims (7)
1. A method for preparing a wound hemostasis dressing by adopting chitosan is characterized by comprising the following steps:
(1) preparing a chitosan hemostatic sponge: adding an acetic acid solution into chitosan powder, oscillating and dissolving at a constant temperature of 40 ℃ to obtain a chitosan acetic acid solution, adding a cross-linking agent into the chitosan acetic acid solution at room temperature, mechanically stirring uniformly, pouring into a culture dish, and freeze-drying in a freeze-drying machine to obtain the chitosan hemostatic sponge;
(2) preparation of regenerated cellulose membrane: putting the dried and activated cellulose into ionic liquid at room temperature, uniformly stirring, gradually raising the speed to 45 ℃, preserving heat for 1h, then slowly adding the ionic liquid, uniformly stirring, preserving heat for 2h at 45 ℃, slowly cooling the cellulose solution to 30 ℃, preheating a glass plate and a wire rod to 30 ℃, pouring the prepared cellulose solution on the glass plate, moving the wire rod, pre-solidifying the formed cellulose solution film at room temperature, and then transferring the glass plate to ultrapure water for solidification to obtain a transparent solid cellulose film;
(3) preparing the composite wound hemostatic dressing: and adhering the chitosan hemostatic sponge to the solid cellulose membrane to obtain the wound hemostatic dressing.
2. The method for preparing a wound hemostatic dressing with chitosan according to claim 1, wherein: and (2) standing at room temperature for defoaming after the chitosan is completely shaken and dissolved.
3. The method for preparing a wound hemostatic dressing with chitosan according to claim 1, wherein: the acetic acid solution in the step (1) is 2% acetic acid solution.
4. The method for preparing a wound hemostatic dressing with chitosan according to claim 1, wherein: the cross-linking agent is any one of 1, 4-butanediol diglycidyl ether, ethylene glycol diglycidyl ether and polyethylene glycol diglycidyl ether.
5. The method for preparing a wound hemostatic dressing with chitosan according to claim 1, wherein: the addition amount of the cross-linking agent is 0.4-1.0% of the mass of the chitosan acetic acid solution.
6. The method for preparing a wound hemostatic dressing with chitosan according to claim 1, wherein: and (3) putting the cellulose into the ionic liquid in the step (2) at room temperature, gradually heating to 45 ℃ at the speed of 3 ℃/min, preserving heat for 1h, then slowly adding the ionic liquid, uniformly stirring, preserving heat for 2h at 45 ℃, stirring once every 20min during the heat preservation period, and slowly cooling the cellulose solution to 30 ℃ at the speed of 0.5 ℃/min.
7. The method for preparing a wound hemostatic dressing with chitosan according to claim 1, wherein: in the step (2), the wire rod is moved at the speed of 1mm/s, the formed cellulose liquid film is pre-solidified for 30min at room temperature, and then the glass plate is transferred into ultrapure water at the temperature of 20 ℃ to be solidified for 30min, so as to obtain a transparent solid cellulose film.
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