CN112972783A - Malignant chest abdomen aquatic separation purification tumor cell device - Google Patents
Malignant chest abdomen aquatic separation purification tumor cell device Download PDFInfo
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- CN112972783A CN112972783A CN201911283759.4A CN201911283759A CN112972783A CN 112972783 A CN112972783 A CN 112972783A CN 201911283759 A CN201911283759 A CN 201911283759A CN 112972783 A CN112972783 A CN 112972783A
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- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 33
- 230000003211 malignant effect Effects 0.000 title claims abstract description 20
- 238000000746 purification Methods 0.000 title claims abstract description 10
- 238000000926 separation method Methods 0.000 title claims description 8
- 210000001015 abdomen Anatomy 0.000 title description 2
- 230000008878 coupling Effects 0.000 claims abstract description 19
- 238000010168 coupling process Methods 0.000 claims abstract description 19
- 238000005859 coupling reaction Methods 0.000 claims abstract description 19
- 206010003445 Ascites Diseases 0.000 claims abstract description 8
- 210000003567 ascitic fluid Anatomy 0.000 claims description 9
- 210000004910 pleural fluid Anatomy 0.000 claims description 9
- 238000003466 welding Methods 0.000 claims description 9
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 6
- 239000012530 fluid Substances 0.000 abstract 2
- 206010026673 Malignant Pleural Effusion Diseases 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000012943 hotmelt Substances 0.000 description 3
- 206010025538 Malignant ascites Diseases 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000001531 micro-dissection Methods 0.000 description 1
- 238000011403 purification operation Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
本发明公开了一种恶性胸腹水中分离纯化肿瘤细胞装置，包括抽吸针，还包括离心装置和离心瓶组件构成，所述的离心装置由固定座、控制面板、伺服电机、联轴器、卡套、放置槽、滑槽、弹簧、卡座和避让孔组成，所述的离心瓶组件由下瓶体、下离心转鼓、过滤层、上瓶体、上离心转鼓和橡胶塞组成，该一种恶性胸腹水中分离纯化肿瘤细胞装置，首先通过前端配合有腹水抽吸器，方便医护人员直接手拿对患者进行细胞提取，其次也利于腹水进入到后续的离心机构，实现细胞分离，最后配合纯化装置的作用，方便无菌PBS液加入，解决了现有方式需要繁琐操作不便临床使用的问题。
The invention discloses a device for separating and purifying tumor cells from malignant pleural and ascites fluid, comprising a suction needle, a centrifugal device and a centrifugal bottle assembly. The centrifugal device is composed of a fixing seat, a control panel, a servo motor, a coupling, It is composed of a ferrule, a placing groove, a chute, a spring, a card seat and an escape hole. The centrifugal bottle assembly is composed of a lower bottle body, a lower centrifugal drum, a filter layer, an upper bottle body, an upper centrifugal drum and a rubber stopper. The device for separating and purifying tumor cells from malignant pleural and ascites fluid is firstly equipped with an ascites aspirator at the front end, which is convenient for medical staff to directly extract cells from patients by hand. Finally, with the function of the purification device, it is convenient to add sterile PBS liquid, and the problem that the existing method requires complicated operations and is inconvenient for clinical use is solved.
Description
Technical Field
The invention relates to equipment for separating and purifying tumor cells, in particular to a device for separating and purifying tumor cells in malignant pleural and peritoneal water.
Background
The effectiveness of the study object can be improved by separating the target tumor cells from the tumor tissues. With the continuous and deep research on tumor, the technology of separating and purifying tumor cells becomes more and more important. At present, a density gradient centrifugation method and a microdissection technology are mainly adopted to separate tumor cells, and an immunomagnetic microsphere method is gradually adopted; in order to purify the tumor cells, other cells can be eliminated by adopting a digestion elimination method, a repeated adherence method, an immunomagnetic microsphere method and the like in the culture process, and the efficient separation and purification of the tumor cells lay the foundation for the later tumor research.
Therefore, the method has important significance for clinical etiology diagnosis, such as disease judgment of tumor cells in malignant pleural effusion and adjustment of clinical treatment strategies, and the tumor cells obtained by separation and purification of malignant pleural effusion are important research tools in drug susceptibility tests and tumor research because the tumor cells directly come from the focus of a patient. Although there are many common methods for isolation and purification from malignant pleural and peritoneal fluid. However, the process of the treatment operation is quite complicated, and corresponding equipment such as the existing centrifugal machine is generally heavy, heavy and complex, is inconvenient to use flexibly, affects the efficiency, improves the cost, and finally greatly restricts the clinical popularization and application.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a device for separating and purifying tumor cells in malignant pleural effusion and abdominal ascites, which solves the problems in the background technology.
In order to solve the technical problems, the technical scheme of the invention is as follows: the utility model provides a malignant chest ascites separation purification tumor cell device, includes the suction needle, still includes centrifugal device and centrifuge bottle subassembly and constitutes, centrifugal device constitute by fixing base, control panel, servo motor, shaft coupling, cutting ferrule, standing groove, spout, spring, cassette and dodge the hole, centrifuge bottle subassembly by lower bottle, lower centrifugal rotary drum, filter layer, go up the bottle, go up centrifugal rotary drum and rubber buffer and constitute, centrifuge bottle subassembly dismantled and assembled with centrifugal device, control panel set firmly in fixing base front end downside, control panel and fixing base adopt bolted connection, servo motor set firmly in the inside left side lower extreme of fixing base, servo motor and fixing base adopt bolted connection, the shaft coupling set firmly in the servo motor top, shaft coupling and servo motor adopt bolted connection, just shaft coupling and fixing base rotate to be connected, the clamping sleeve is fixedly arranged at the top of the coupler, the clamping sleeve is connected with the coupler in a welding manner, the clamping sleeve is rotatably connected with the fixed seat, the placing groove is positioned at the left side inside the fixed seat, the chute is positioned at the upper end of the right side inside the fixed seat, the chute is a rectangular groove, the spring is fixedly arranged at the lower end inside the chute, the spring is connected with the fixed seat in a welding manner, the clamping seat is slidably arranged inside the chute, the clamping seat is connected with the fixed seat in a vertical sliding manner, the clamping seat is connected with the spring in a welding manner, the avoiding hole is positioned at the top end of the left side inside the clamping seat, the avoiding hole is a circular through hole, the lower bottle body is positioned at the upper end inside the clamping sleeve and is movably connected with the clamping sleeve, the lower centrifugal rotary drum is fixedly arranged inside the lower bottle body, and the lower centrifugal, the filter layer set firmly in the inside middle-end of centrifugal drum down, the filter layer adopt medical level adhesive with lower centrifugal drum to be connected, last bottle be located lower bottle upper end, last bottle adopt bayonet coupling with lower bottle, last centrifugal drum set firmly inside last bottle, last centrifugal drum and last bottle heat-fusing be connected, rubber buffer quantity be two, the rubber buffer set firmly respectively in the port department of lower bottle and last bottle, the rubber buffer respectively with lower bottle with last bottle heat-fusing be connected.
Further, the fixing base right side still set firmly the handle, handle and fixing base integrated into one piece, the inside right side lower extreme of fixing base still set firmly the battery, battery and fixing base adopt bolted connection, the battery right side still set firmly the interface that charges, interface and the fixing base that charges adopt bolted connection, just interface and the battery that charges adopt the electrical conductor to be connected.
Furthermore, the control panel comprises a PLC processor, an A/D converter, a display screen, a servo driver and a delay relay, wherein the servo driver and the delay relay are respectively connected with the servo motor by signal lines.
Further, a pulling handle is fixedly arranged on the right side of the top of the clamping seat, and the pulling handle and the clamping seat are integrally formed.
Furthermore, a clamping groove is further formed in the clamping sleeve, the clamping groove is a triangular groove, and the clamping groove is connected with the lower bottle body in a vertical sliding mode.
Compared with the prior art, this malignant pleural effusion and peritoneal water separation purification tumor cells device has the ascites aspirator through the front end cooperation at first, makes things convenient for medical personnel to directly take and carries out the cell extraction to the patient, and secondly also does benefit to the ascites and enters into subsequent centrifugal mechanism, realizes the cell separation, cooperates the effect of purification device at last, and convenient aseptic PBS liquid is added, has solved the problem that current mode needs the inconvenient clinical use of loaded down with trivial details operation.
Drawings
FIG. 1 is a front view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal water;
FIG. 2 is a side view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal water;
FIG. 3 is a perspective view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal fluid, which is shown in FIG. 1;
FIG. 4 is a perspective view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal fluid, FIG. 2;
FIG. 5 is a perspective view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal fluid, which is shown in FIG. 3;
FIG. 6 is a perspective view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal fluid, which is shown in FIG. 4;
FIG. 7 is a perspective view of an apparatus for separating and purifying tumor cells in malignant pleural and peritoneal fluid, which is shown in FIG. 5;
FIG. 8 is a sectional view of the device for separating and purifying tumor cells in malignant pleural and peritoneal water in the direction A;
fig. 9 is a top view of the ferrule.
The device comprises a suction needle 1, a fixed seat 2, a control panel 3, a servo motor 4, a coupler 5, a clamping sleeve 6, a placing groove 7, a sliding groove 8, a spring 9, a clamping seat 10, an avoiding hole 11, a lower bottle body 12, a lower centrifugal drum 13, a filter layer 14, an upper bottle body 15, an upper centrifugal drum 16, a rubber plug 17, a handle 201, a storage battery 202, a charging interface 203, a clamping groove 601 and a pulling handle 1001.
The following detailed description will be further described in conjunction with the above-identified drawings.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the concepts underlying the described embodiments, however, it will be apparent to one skilled in the art that the described embodiments may be practiced without some or all of these specific details, and in other cases well-known process steps have not been described in detail.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8, and fig. 9, a device for separating and purifying tumor cells in malignant pleural and peritoneal water comprises a suction needle 1, a fixing base 2, a control panel 3, a servo motor 4, a coupling 5, a ferrule 6, a placement groove 7, a sliding groove 8, a spring 9, a clamping base 10, an avoiding hole 11, a lower bottle body 12, a lower centrifugal drum 13, a filter layer 14, an upper bottle body 15, an upper centrifugal drum 16, and a rubber plug 17, wherein the centrifugal bottle assembly is detachable from the centrifugal device, the control panel 3 is fixedly arranged at the lower side of the front end of the fixing base 2, the control panel 3 is connected with the fixing base 2 by bolts, the servo motor 4 is fixedly arranged at the lower end of the left side inside the fixing base 2, the servo motor 4 is connected with the fixing base 2 by bolts, the coupling 5 is fixedly arranged at the top of the servo motor 4, the, the coupling 5 is rotatably connected with the fixed seat 2, the cutting sleeve 6 is fixedly arranged at the top of the coupling 5, the cutting sleeve 6 is connected with the coupling 5 in a welding manner, the cutting sleeve 6 is rotatably connected with the fixed seat 2, the placing groove 7 is arranged at the left side inside the fixed seat 2, the chute 8 is arranged at the upper end of the right side inside the fixed seat 2, the chute 8 is a rectangular groove, the spring 9 is fixedly arranged at the lower end inside the chute 8, the spring 9 is connected with the fixed seat 2 in a welding manner, the clamping seat 10 is slidably arranged inside the chute 8, the clamping seat 10 is connected with the fixed seat 2 in a vertical sliding manner, the clamping seat 10 is connected with the spring 9 in a welding manner, the avoiding hole 11 is arranged at the top end of the left side inside the clamping seat 10, the avoiding hole 11 is a circular through hole, the lower bottle body 12 is arranged at the upper end inside the cutting sleeve 6, and the lower bottle body 12 is, lower centrifugal drum 13 set firmly in lower bottle 12 inside, lower centrifugal drum 13 and lower bottle 12 hot melt be connected, filter layer 14 set firmly in the inside middle-end of lower centrifugal drum 13, filter layer 14 and lower centrifugal drum 13 adopt medical grade adhesive to be connected, last bottle 15 be located lower bottle 12 upper end, last bottle 15 and lower bottle 12 adopt bayonet coupling, last centrifugal drum 16 set firmly in last bottle 15 inside, last centrifugal drum 16 and last bottle 15 hot melt be connected, rubber buffer 17 quantity be two, rubber buffer 17 set firmly respectively in the port department of lower bottle 12 and last bottle 15, rubber buffer 17 respectively with lower bottle 12 and last bottle 15 hot melt be connected;
before centrifugation, malignant pleural effusion and ascites are collected through operation, the suction needle 1 is inserted into the rubber plug 17, then the malignant pleural effusion and ascites can be introduced into the centrifuge bottle assembly, then the clamping seat 10 is pulled upwards, the clamping seat 10 moves from bottom to top along the sliding groove 8 to overcome the resilience force of the spring 9, the fixed seat 2 is opened, then the centrifuge bottle assembly is placed into the placing groove 7, the lower bottle body 12 is inserted into the clamping groove 601 in the clamping sleeve 6, then the medical personnel loosens the clamping seat 10, namely the clamping seat 10 is reset downwards through the resilience force of the spring 9, the clamping seat 10 can be sleeved outside the top of the upper bottle body 15 under the action of the avoiding hole 11 in the clamping seat 10, so that the centrifuge bottle assembly is fixed, and at the moment, the medical personnel can conveniently extract and place cells through the operation; during centrifugation, medical personnel operate the control panel 3 to realize the triggering and opening of the servo motor 4, namely the servo motor 4 drives the coupler 5 to link the centrifugal bottle assembly at the upper end of the clamping sleeve 6, namely under the action of centrifugal force generated by ultra-high speed rotation, cell liquid in the centrifugal rotary drum is conveniently separated, namely suspension is separated into the centrifugal bottles; during heavy suspension, the exposure of the rubber plug 17 is realized due to the action of the avoidance hole 11 at the upper end of the clamping seat 10, namely, the suction needle 1 can be inserted into the rubber plug 17 through the avoidance hole 11 again, then sterile PBS liquid is injected, so that the liquid enters the lower bottle body 12 through the upper bottle body 15, then tumor cell suspension can be obtained, due to the action of the filter layer 14 at the middle end of the centrifugal bottle, an upper solution and a lower solution can be formed, the upper solution is tumor cell suspension, the lower solution is serum, then the centrifugal bottle assembly is kept stand for about 5 minutes and then taken out by medical personnel, then the traditional syringe is inserted into the rubber plug 17 at the bottom port of the lower bottle body 12, then the serum at the bottom of the bottle is sucked, at the moment, the liquid layering cannot be damaged due to the requirement at the lower end, then the subsequent conventional purification operation can be carried out, namely, the taken-, centrifuging at normal temperature, and removing supernatant to obtain purified tumor cells;
the right side of the fixed seat 2 is fixedly provided with a handle 201, the handle 201 and the fixed seat 2 are integrally formed, the lower end of the right side in the fixed seat 2 is fixedly provided with a storage battery 202, the storage battery 202 is connected with the fixed seat 2 through bolts, the right side of the storage battery 202 is fixedly provided with a charging interface 203, the charging interface 203 is connected with the fixed seat 2 through bolts, and the charging interface 203 is connected with the storage battery 202 through electric conductors;
it should be noted that the handle 201 is for facilitating the medical staff to carry the device, the storage battery 202 is for providing energy supply to the servo motor 4 and the control panel 3, and the charging interface 203 is for connecting an external power supply, so as to facilitate the charging operation of the storage battery 202;
the control panel 3 consists of a PLC processor, an A/D converter, a display screen, a servo driver and a delay relay, wherein the servo driver and the delay relay are respectively connected with the servo motor 4 by signal lines;
it should be noted that the PLC processor is for processing the converted numerical signal of the a/D converter, the model of the PLC processor is FX1N-06MR, the model of the a/D converter is TELESKY, the display screen is for displaying the rotation speed parameter, the model of the display screen is KD070HDTLA020-TP, the servo driver is for adjusting the rotation speed of the servo motor 4, the model of the servo driver is teda svga, the model of the servo motor is teda svga, the delay relay is for setting the centrifugal time for medical personnel, and the model of the delay relay is ohron H3Y-2H3Y-4, so as to sum up, the medical personnel can set the detailed parameters such as time and speed, for example, the conventional parameter is 1000-;
a pulling handle 1001 is fixedly arranged on the right side of the top of the clamping seat 10, and the pulling handle 1001 and the clamping seat 10 are integrally formed;
it should be noted that the pulling handle 1001 is for facilitating the medical staff to pull the card seat 10, so as to improve the convenience of operation;
a clamping groove 601 is further formed in the clamping sleeve 6, the clamping groove 601 is a triangular groove, and the clamping groove 601 is connected with the lower bottle body 12 in a vertically sliding mode;
it should be noted that the clamping groove 601 is a triangular groove, so that the clamping groove can be conveniently connected with the end of the lower bottle body 12 with a triangular shape, and the clamping sleeve 6 drives the centrifuge bottle assembly to synchronously rotate.
Claims (5)
1. The utility model provides a malignant chest ascites separation purification tumor cell device, includes the suction needle, its characterized in that still includes centrifugal device and centrifuge bottle subassembly and constitutes, centrifugal device constitute by fixing base, control panel, servo motor, shaft coupling, cutting ferrule, standing groove, spout, spring, cassette and dodge the hole, centrifuge bottle subassembly by lower bottle, lower centrifugal rotary drum, filter layer, go up the bottle, go up centrifugal rotary drum and rubber buffer and constitute, centrifuge bottle subassembly dismantled and centrifuge device, control panel set firmly in fixing base front end downside, control panel and fixing base adopt bolted connection, servo motor set firmly in the inside left side lower extreme of fixing base, servo motor and fixing base adopt bolted connection, the shaft coupling set firmly in the servo motor top, shaft coupling and servo motor adopt bolted connection, the shaft coupling is rotatably connected with the fixed seat, the cutting sleeve is fixedly arranged at the top of the shaft coupling, the cutting sleeve is connected with the shaft coupling in a welding manner, the cutting sleeve is rotatably connected with the fixed seat, the placing groove is positioned at the left side inside the fixed seat, the chute is positioned at the upper end of the right side inside the fixed seat, the chute is a rectangular groove, the spring is fixedly arranged at the lower end inside the chute, the spring is connected with the fixed seat in a welding manner, the clamping seat is slidably arranged inside the chute, the clamping seat is connected with the fixed seat in a vertical sliding manner, the clamping seat is connected with the spring in a welding manner, the avoiding hole is positioned at the top end of the left side inside the clamping seat, the avoiding hole is a circular through hole, the lower bottle body is positioned at the upper end inside the cutting sleeve, the lower bottle body is movably connected with the cutting sleeve, the lower centrifugal drum is fixedly arranged inside the lower, the filter layer set firmly in the inside middle-end of centrifugal drum down, the filter layer adopt medical level adhesive with lower centrifugal drum to be connected, last bottle be located lower bottle upper end, last bottle adopt bayonet coupling with lower bottle, last centrifugal drum set firmly inside last bottle, last centrifugal drum and last bottle heat-fusing be connected, rubber buffer quantity be two, the rubber buffer set firmly respectively in the port department of lower bottle and last bottle, the rubber buffer respectively with lower bottle with last bottle heat-fusing be connected.
2. The device for separating and purifying tumor cells in malignant pleural and peritoneal fluid of claim 1, wherein a handle is further fixed to the right side of the holder, the handle is integrally formed with the holder, a storage battery is further fixed to the lower end of the right side inside the holder, the storage battery is connected to the holder through a bolt, a charging interface is further fixed to the right side of the storage battery, the charging interface is connected to the holder through a bolt, and the charging interface is connected to the storage battery through an electrical lead.
3. The apparatus according to claim 1, wherein the control panel comprises a PLC processor, an a/D converter, a display, a servo driver and a delay relay, the servo driver and the delay relay are respectively connected to the servo motor via signal lines.
4. The apparatus for separating and purifying tumor cells in malignant pleural and peritoneal fluid of claim 1, wherein a pull handle is further fixed on the right side of the top of the holder, and the pull handle and the holder are integrally formed.
5. The device for separating and purifying tumor cells in malignant pleural and peritoneal fluid of claim 1, wherein the clamping sleeve is further provided with a clamping groove therein, the clamping groove is a triangular groove, and the clamping groove is connected with the lower bottle body in a sliding manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911283759.4A CN112972783B (en) | 2019-12-13 | 2019-12-13 | Device for separating and purifying tumor cells in malignant hydrothorax and ascites |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911283759.4A CN112972783B (en) | 2019-12-13 | 2019-12-13 | Device for separating and purifying tumor cells in malignant hydrothorax and ascites |
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| CN112972783A true CN112972783A (en) | 2021-06-18 |
| CN112972783B CN112972783B (en) | 2024-04-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120284333A (en) * | 2025-06-09 | 2025-07-11 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | A body fluid sample collection device for pleural effusion and ascites and a method of using the same |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002081095A1 (en) * | 2001-04-09 | 2002-10-17 | Medtronic, Inc. | Microncentrifuge and drive therefor |
| JP2005279507A (en) * | 2004-03-30 | 2005-10-13 | Matsushita Electric Ind Co Ltd | Microbial extraction method by density gradient centrifugation |
| JP2006020658A (en) * | 2004-07-05 | 2006-01-26 | Koshin Kogyo:Kk | Medical electric aspirator |
| WO2009031990A1 (en) * | 2005-04-20 | 2009-03-12 | Millennium Medical Technologies, Inc. | Fluid concentrator |
| JP2010115175A (en) * | 2008-11-14 | 2010-05-27 | Olympus Corp | Centrifugal separation device and centrifugal separation method |
| RU97043U1 (en) * | 2009-02-27 | 2010-08-27 | Государственное образовательное учреждение высшего профессионального образования Военно-медицинская академия им.С.М.Кирова | DEVICE FOR CONCENTRATION AND FILTRATION OF ASCITIC LIQUID |
| JP2011004705A (en) * | 2009-06-29 | 2011-01-13 | Olympus Corp | Cell separation device and cell separation method |
| JP2012095618A (en) * | 2010-11-04 | 2012-05-24 | Olympus Corp | Cell separation apparatus |
| US20120165177A1 (en) * | 2009-07-31 | 2012-06-28 | Tetsuya Tsugawa | Vertical centrifugal separation apparatus and method of recovering centrifuged liquid |
| WO2013141436A1 (en) * | 2012-03-19 | 2013-09-26 | (주)세원메디텍 | Blood centrifugation container |
| CN104962520A (en) * | 2015-06-12 | 2015-10-07 | 广州医科大学附属第一医院 | Method for fast and efficient separation and purification of tumor cells from malignant pleural effusion and ascites |
| CN204779578U (en) * | 2015-06-08 | 2015-11-18 | 沈阳市第四人民医院 | Draw drop filter equipment of cell of chest ascites |
| CN107432958A (en) * | 2017-08-13 | 2017-12-05 | 李凯 | Wet lung drainage arrangement |
| US20180221550A1 (en) * | 2011-07-08 | 2018-08-09 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| CN110192914A (en) * | 2019-06-26 | 2019-09-03 | 厦门市第五医院(厦门市同民医院) | A kind of tumor operation hydrops extractor tool |
| CN110257241A (en) * | 2019-07-04 | 2019-09-20 | 北京晶科瑞医学检验实验室有限公司 | A kind of device of the quick separating tumour cell from lung's malignant hydrothorax |
| CN209589868U (en) * | 2019-03-06 | 2019-11-05 | 成都中珠健联基因科技有限责任公司 | The extraction element of high efficiency extraction tumour cell |
-
2019
- 2019-12-13 CN CN201911283759.4A patent/CN112972783B/en active Active
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002081095A1 (en) * | 2001-04-09 | 2002-10-17 | Medtronic, Inc. | Microncentrifuge and drive therefor |
| JP2005279507A (en) * | 2004-03-30 | 2005-10-13 | Matsushita Electric Ind Co Ltd | Microbial extraction method by density gradient centrifugation |
| JP2006020658A (en) * | 2004-07-05 | 2006-01-26 | Koshin Kogyo:Kk | Medical electric aspirator |
| WO2009031990A1 (en) * | 2005-04-20 | 2009-03-12 | Millennium Medical Technologies, Inc. | Fluid concentrator |
| JP2010115175A (en) * | 2008-11-14 | 2010-05-27 | Olympus Corp | Centrifugal separation device and centrifugal separation method |
| RU97043U1 (en) * | 2009-02-27 | 2010-08-27 | Государственное образовательное учреждение высшего профессионального образования Военно-медицинская академия им.С.М.Кирова | DEVICE FOR CONCENTRATION AND FILTRATION OF ASCITIC LIQUID |
| JP2011004705A (en) * | 2009-06-29 | 2011-01-13 | Olympus Corp | Cell separation device and cell separation method |
| US20120165177A1 (en) * | 2009-07-31 | 2012-06-28 | Tetsuya Tsugawa | Vertical centrifugal separation apparatus and method of recovering centrifuged liquid |
| JP2012095618A (en) * | 2010-11-04 | 2012-05-24 | Olympus Corp | Cell separation apparatus |
| US20180221550A1 (en) * | 2011-07-08 | 2018-08-09 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| WO2013141436A1 (en) * | 2012-03-19 | 2013-09-26 | (주)세원메디텍 | Blood centrifugation container |
| CN204779578U (en) * | 2015-06-08 | 2015-11-18 | 沈阳市第四人民医院 | Draw drop filter equipment of cell of chest ascites |
| CN104962520A (en) * | 2015-06-12 | 2015-10-07 | 广州医科大学附属第一医院 | Method for fast and efficient separation and purification of tumor cells from malignant pleural effusion and ascites |
| CN107432958A (en) * | 2017-08-13 | 2017-12-05 | 李凯 | Wet lung drainage arrangement |
| CN209589868U (en) * | 2019-03-06 | 2019-11-05 | 成都中珠健联基因科技有限责任公司 | The extraction element of high efficiency extraction tumour cell |
| CN110192914A (en) * | 2019-06-26 | 2019-09-03 | 厦门市第五医院(厦门市同民医院) | A kind of tumor operation hydrops extractor tool |
| CN110257241A (en) * | 2019-07-04 | 2019-09-20 | 北京晶科瑞医学检验实验室有限公司 | A kind of device of the quick separating tumour cell from lung's malignant hydrothorax |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120284333A (en) * | 2025-06-09 | 2025-07-11 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | A body fluid sample collection device for pleural effusion and ascites and a method of using the same |
| CN120284333B (en) * | 2025-06-09 | 2025-10-17 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | Body fluid sample collection device for hydrothorax and ascites and application method thereof |
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