CN112972783B - Device for separating and purifying tumor cells in malignant hydrothorax and ascites - Google Patents
Device for separating and purifying tumor cells in malignant hydrothorax and ascites Download PDFInfo
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- CN112972783B CN112972783B CN201911283759.4A CN201911283759A CN112972783B CN 112972783 B CN112972783 B CN 112972783B CN 201911283759 A CN201911283759 A CN 201911283759A CN 112972783 B CN112972783 B CN 112972783B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
本发明公开了一种恶性胸腹水中分离纯化肿瘤细胞装置，包括抽吸针，还包括离心装置和离心瓶组件构成，所述的离心装置由固定座、控制面板、伺服电机、联轴器、卡套、放置槽、滑槽、弹簧、卡座和避让孔组成，所述的离心瓶组件由下瓶体、下离心转鼓、过滤层、上瓶体、上离心转鼓和橡胶塞组成，该一种恶性胸腹水中分离纯化肿瘤细胞装置，首先通过前端配合有腹水抽吸器，方便医护人员直接手拿对患者进行细胞提取，其次也利于腹水进入到后续的离心机构，实现细胞分离，最后配合纯化装置的作用，方便无菌PBS液加入，解决了现有方式需要繁琐操作不便临床使用的问题。
The invention discloses a device for separating and purifying tumor cells from malignant pleural and abdominal effusions. The device comprises an aspiration needle, a centrifugal device and a centrifugal bottle assembly. The centrifugal device comprises a fixing seat, a control panel, a servo motor, a coupling, a ferrule, a placement groove, a slide groove, a spring, a ferrule and an avoidance hole. The centrifugal bottle assembly comprises a lower bottle body, a lower centrifugal drum, a filter layer, an upper bottle body, an upper centrifugal drum and a rubber plug. The device for separating and purifying tumor cells from malignant pleural and abdominal effusions firstly cooperates with an ascites aspirator at the front end to facilitate medical staff to directly extract cells from patients by hand. Secondly, it is also beneficial for ascites to enter a subsequent centrifugal mechanism to achieve cell separation. Finally, in conjunction with the function of the purification device, it is convenient to add sterile PBS liquid, thereby solving the problem that the existing method requires cumbersome operations and is inconvenient for clinical use.
Description
Technical Field
The invention relates to a device for separating and purifying tumor cells, in particular to a device for separating and purifying tumor cells in malignant hydrothorax and ascites.
Background
The target tumor cells are separated from the tumor tissue, so that the effectiveness of the study object can be improved. With the continuous deep research on tumor, the separation and purification technology of tumor cells is more and more important. At present, a density gradient centrifugation method and a microdissection technology are mainly adopted to separate tumor cells, and an immunomagnetic microsphere method is gradually adopted; in order to purify the tumor cells, other cells can be removed by adopting a digestion removal method, a repeated adherence method, an immunomagnetic microsphere method and the like in the culture process, and the efficient separation and purification of the tumor cells lays a foundation for the later tumor research.
Therefore, the method has important significance for clinical etiology diagnosis, such as disease judgment of tumor cells in malignant hydrothorax and ascites and adjustment of clinical treatment strategies, and meanwhile, the tumor cells obtained by separation and purification from the malignant hydrothorax and ascites are important research tools in drug sensitivity tests and tumor research because the tumor cells are directly derived from lesions of patients. Although there are many methods commonly used today for separation and purification from malignant hydrothorax and ascites. However, the process of the treatment operation is quite complicated, corresponding equipment such as the existing centrifugal machine is generally heavy and complex, the equipment is inconvenient to use flexibly, the efficiency is also influenced, the cost is increased, the clinical popularization and application are finally greatly restricted, and in view of the defects, a device for separating and purifying tumor cells in malignant hydrothorax and ascites is needed to be designed.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: provides a device for separating and purifying tumor cells in malignant hydrothorax and ascites, which solves the problems proposed by the background technology.
In order to solve the technical problems, the technical scheme of the invention is as follows: the utility model provides a separate purification tumour cell device in malignant chest and ascites, includes the suction needle, still includes centrifugal device and centrifugation bottle subassembly constitutes, centrifugal device by fixing base, control panel, servo motor, shaft coupling, cutting ferrule, standing groove, spout, spring, cassette and dodge the hole and constitute, centrifugation bottle subassembly by lower bottle, lower centrifugation rotary drum, filter layer, last bottle, last centrifugation rotary drum and rubber buffer constitute, centrifugation bottle subassembly and centrifugal device detachable, control panel set firmly in fixing base front end downside, control panel and fixing base adopt bolted connection, servo motor set firmly in fixing base inside left side lower extreme, servo motor and fixing base adopt bolted connection, the shaft coupling set firmly in servo motor top, shaft coupling and servo motor adopt bolted connection, and shaft coupling and fixing base rotate to be connected, the clamping sleeve is fixedly arranged at the top of the coupling, the clamping sleeve is in welded connection with the coupling, the clamping sleeve is in rotary connection with the fixing seat, the placing groove is positioned at the left side inside the fixing seat, the sliding groove is positioned at the right upper end inside the fixing seat, the sliding groove is a rectangular groove, the spring is fixedly arranged at the lower end inside the sliding groove, the spring is in welded connection with the fixing seat, the clamping seat is slidingly arranged inside the sliding groove, the clamping seat is in vertical sliding connection with the fixing seat, the clamping seat is in welded connection with the spring, the avoiding hole is positioned at the top end inside the clamping seat, the avoiding hole is a circular through hole, the lower bottle body is positioned at the upper end inside the clamping sleeve, the lower bottle body is in movable connection with the clamping sleeve, the lower centrifugal drum is fixedly arranged inside the lower bottle body, the lower centrifugal drum is in hot melt connection with the lower bottle body, the filter layer set firmly in the inside middle-end of lower centrifugal rotary drum, filter layer and lower centrifugal rotary drum adopt medical level adhesive to be connected, last bottle be located lower bottle upper end, last bottle and lower bottle adopt bayonet connection, last centrifugal rotary drum set firmly in the interior of last bottle, last centrifugal rotary drum and last bottle hot melt connection, rubber buffer quantity be two, the rubber buffer set firmly in the port department of lower bottle and last bottle respectively, the rubber buffer be connected with lower bottle and last bottle hot melt respectively.
Further, the fixing base right side still set firmly the handle, handle and fixing base integrated into one piece, the inside right side lower extreme of fixing base still set firmly the battery, battery and fixing base adopt bolted connection, battery right side still set firmly the interface that charges, the interface that charges adopt bolted connection with the fixing base, just the interface that charges adopt electrically conductive wire connection with the battery.
Further, the control panel consists of a PLC processor, an A/D converter, a display screen, a servo driver and a delay relay, wherein the servo driver and the delay relay are respectively connected with a servo motor through signal wires.
Further, the right side of the top of the clamping seat is fixedly provided with a pulling handle, and the pulling handle and the clamping seat are integrally formed.
Further, the inside draw-in groove that still is equipped with of cutting ferrule, the draw-in groove be triangle-shaped recess, just draw-in groove and lower bottle from top to bottom sliding connection.
Compared with the prior art, the device for separating and purifying tumor cells in malignant hydrothorax and ascites has the advantages that firstly, the front end is matched with the ascites aspirator, so that medical staff can directly take the cells of a patient, secondly, the ascites can enter a follow-up centrifugal mechanism, cell separation is realized, finally, the effect of a purifying device is matched, the sterile PBS liquid is conveniently added, and the problem that complicated operation is required in the existing mode and the clinical use is inconvenient is solved.
Drawings
FIG. 1 is a front view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 2 is a side view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 3 is a perspective view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 4 is a perspective view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 5 is a perspective view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 6 is a perspective view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 7 is a perspective view of an apparatus for separating and purifying tumor cells from malignant hydrothorax and ascites;
FIG. 8 is an A-direction cross-sectional view of a device for separating and purifying tumor cells in malignant hydrothorax and ascites;
fig. 9 is a top view of the ferrule.
The suction needle 1, the fixing seat 2, the control panel 3, the servo motor 4, the coupler 5, the clamping sleeve 6, the placing groove 7, the sliding chute 8, the spring 9, the clamping seat 10, the avoiding hole 11, the lower bottle body 12, the lower centrifugal rotary drum 13, the filter layer 14, the upper bottle body 15, the upper centrifugal rotary drum 16, the rubber plug 17, the handle 201, the storage battery 202, the charging interface 203, the clamping groove 601 and the pulling handle 1001.
The following detailed description will be further described in conjunction with the above-described figures.
Detailed Description
In the following, numerous specific details are set forth in order to provide a thorough understanding of the concepts underlying the described embodiments, however, it will be apparent to one skilled in the art that the described embodiments may be practiced without some or all of these specific details, and in other instances well-known process steps are not described in detail.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8, fig. 9, a device for separating and purifying tumor cells in malignant hydrothorax and ascites comprises a suction needle 1, a fixed seat 2, a control panel 3, a servo motor 4, a coupler 5, a clamping sleeve 6, a placing groove 7, a chute 8, a spring 9, a clamping seat 10, a avoiding hole 11, a lower bottle body 12, a lower centrifugal drum 13, a filter layer 14, an upper bottle body 15, an upper centrifugal drum 16 and a rubber plug 17, wherein the centrifugal bottle assembly is detachable with a centrifugal device, the control panel 3 is fixedly arranged at the lower side of the front end of the fixed seat 2, the control panel 3 is connected with the fixed seat 2 through bolts, the servo motor 4 is fixedly arranged at the lower end of the left side inside of the fixed seat 2, the servo motor 4 is connected with the fixed seat 2 through bolts, the coupler 5 is fixedly arranged at the top of the servo motor 4, the coupler 5 is connected with the servo motor 4 through bolts, the coupling 5 is rotationally connected with the fixed seat 2, the clamping sleeve 6 is fixedly arranged at the top of the coupling 5, the clamping sleeve 6 is rotationally connected with the fixed seat 2, the placing groove 7 is positioned at the left side inside the fixed seat 2, the sliding groove 8 is positioned at the right upper end inside the fixed seat 2, the sliding groove 8 is a rectangular groove, the spring 9 is fixedly arranged at the lower end inside the sliding groove 8, the spring 9 is in welded connection with the fixed seat 2, the clamping seat 10 is slidingly arranged inside the sliding groove 8, the clamping seat 10 is in up-down sliding connection with the fixed seat 2, the clamping seat 10 is in welded connection with the spring 9, the avoiding hole 11 is positioned at the left top inside the clamping seat 10, the avoiding hole 11 is a circular through hole, the lower bottle body 12 is positioned at the upper end inside the clamping sleeve 6, the lower bottle body 12 is movably connected with the clamping sleeve 6, the lower centrifugal rotary drum 13 is fixedly arranged in the lower bottle body 12, the lower centrifugal rotary drum 13 is in hot melting connection with the lower bottle body 12, the filter layer 14 is fixedly arranged at the middle end in the lower centrifugal rotary drum 13, the filter layer 14 is connected with the lower centrifugal rotary drum 13 through a medical grade adhesive, the upper bottle body 15 is positioned at the upper end of the lower bottle body 12, the upper bottle body 15 is in bayonet connection with the lower bottle body 12, the upper centrifugal rotary drum 16 is fixedly arranged in the upper bottle body 15, the upper centrifugal rotary drum 16 is in hot melting connection with the upper bottle body 15, the number of rubber plugs 17 is two, the rubber plugs 17 are respectively fixedly arranged at ports of the lower bottle body 12 and the upper bottle body 15, and the rubber plugs 17 are respectively in hot melting connection with the lower bottle body 12 and the upper bottle body 15;
before centrifugation, malignant hydrothorax and ascites are collected through operation, the suction needle 1 is inserted into the rubber plug 17, then the malignant hydrothorax and ascites can be led into the centrifugal bottle assembly, then the clamping seat 10 is pulled upwards, the clamping seat 10 moves downwards along the chute 8 against the resilience force of the spring 9, the fixed seat 2 is opened, the centrifugal bottle assembly is placed into the placing groove 7, the lower bottle body 12 is inserted above the clamping groove 601 in the clamping sleeve 6, then the clamping seat 10 is loosened by medical staff, namely, the clamping seat 10 is reset downwards through the resilience force of the spring 9, and the inside of the clamping seat 10 can be sleeved outside the top of the upper bottle body 15 due to the action of the avoidance hole 11, so that the centrifugal bottle assembly is fixed, and at the moment, the cell is conveniently extracted and placed by medical staff through the operation; during centrifugation, medical staff operates the control panel 3 to realize that the servo motor 4 is triggered and started, namely the servo motor 4 drives the upper end of the coupling 5 to link the clamping sleeve 6 to form a centrifugal bottle component, namely under the action of centrifugal force generated by ultra-high-speed rotation, so that cell liquid separation in the centrifugal drum is facilitated, namely suspension is separated into the centrifugal bottle; during resuspension, the avoidance hole 11 at the upper end of the clamping seat 10 acts, so that the rubber plug 17 is exposed, namely the suction needle 1 can be inserted into the rubber plug 17 through the avoidance hole 11 again, sterile PBS liquid is injected, the liquid enters the lower bottle body 12 through the upper bottle body 15, then tumor cell suspension can be obtained, an upper layer and a lower layer of solution can be formed due to the effect of the filter layer 14 at the middle end of the centrifugal bottle, the upper layer of solution is tumor cell suspension, the lower layer of solution is serum, after standing for about 5 minutes, a medical staff takes out the centrifugal bottle assembly again, then the conventional syringe is inserted into the rubber plug 17 at the bottom port of the lower bottle body 12, then serum at the bottom of the bottle is sucked, at the moment, the lower end needs, the delamination of the liquid cannot be destroyed, then the subsequent conventional purification operation can be carried out, namely the extracted serum is placed in a new centrifuge tube, the sterile PBS liquid is added, the normal temperature is centrifuged, and the supernatant is discarded, and finally the purified tumor cells can be obtained;
the right side of the fixed seat 2 is fixedly provided with a handle 201, the handle 201 and the fixed seat 2 are integrally formed, the lower end of the right side inside the fixed seat 2 is fixedly provided with a storage battery 202, the storage battery 202 is connected with the fixed seat 2 by adopting a bolt, the right side of the storage battery 202 is fixedly provided with a charging interface 203, the charging interface 203 is connected with the fixed seat 2 by adopting a bolt, and the charging interface 203 is connected with the storage battery 202 by adopting an electric wire;
it should be noted that the handle 201 is for facilitating the medical staff to carry the device, the storage battery 202 is for providing energy supply for the servo motor 4 and the control panel 3, and the charging interface 203 is for connecting with an external power supply, so as to facilitate the charging operation of the storage battery 202;
the control panel 3 consists of a PLC processor, an A/D converter, a display screen, a servo driver and a delay relay, wherein the servo driver and the delay relay are respectively connected with a servo motor 4 by adopting signal wires;
the PLC processor is used for processing a converted numerical signal of the A/D converter, the PLC processor is of a programmable controller FX1N-06MR, the A/D converter is of a TELESKY type, the display screen is used for displaying rotating speed parameters, the display screen is of KD070HDTLA020-TP, the servo driver is used for conveniently adjusting the rotating speed of the servo motor 4, the servo driver is of a Tuodsva type, the servo motor is of a Tuodsva type, the delay relay is used for conveniently setting the centrifugal time of medical staff, the delay relay is of an ohm dragon H3Y-2H3Y-4, and in combination, the medical staff is convenient to set the time, the speed and other detailed parameters, for example, the conventional parameters are of the rotating speed 1000-1500r/min, and the centrifugal time is about 5 minutes;
a pulling handle 1001 is also fixedly arranged on the right side of the top of the clamping seat 10, and the pulling handle 1001 and the clamping seat 10 are integrally formed;
it should be noted that, the pulling handle 1001 is for facilitating the medical staff to pull the card holder 10, so as to improve the operation convenience;
a clamping groove 601 is further formed in the clamping sleeve 6, the clamping groove 601 is a triangular groove, and the clamping groove 601 is connected with the lower bottle body 12 in an up-down sliding mode;
it should be noted that, because the card slot 601 is a triangle groove, the card slot can be conveniently connected with the port of the lower bottle body 12 with a triangle shape, and the card sleeve 6 can drive the centrifugal bottle assembly to synchronously rotate.
Claims (5)
1. The utility model provides a separate purification tumour cell device in malignant chest and ascites, includes the suction needle, and its characterized in that still includes centrifugal device and centrifugation bottle subassembly, centrifugal device by fixing base, control panel, servo motor, shaft coupling, cutting ferrule, standing groove, spout, spring, cassette and dodge the hole and constitute, centrifugation bottle subassembly by lower bottle, lower centrifugation rotary drum, filter layer, last bottle, last centrifugation rotary drum and rubber buffer constitute, centrifugation bottle subassembly and centrifugal device detachable, control panel set firmly in fixing base front end downside, control panel and fixing base adopt bolted connection, servo motor set firmly in fixing base inside left side lower extreme, servo motor and fixing base adopt bolted connection, the shaft coupling set firmly in servo motor top, shaft coupling and servo motor adopt bolted connection, the coupling is rotationally connected with the fixing seat, the clamping sleeve is fixedly arranged at the top of the coupling, the clamping sleeve is in welded connection with the coupling, the clamping sleeve is rotationally connected with the fixing seat, the placing groove is positioned at the left side inside the fixing seat, the sliding groove is positioned at the right side upper end inside the fixing seat, the sliding groove is a rectangular groove, the spring is fixedly arranged at the lower end inside the sliding groove, the spring is in welded connection with the fixing seat, the clamping seat is slidably arranged inside the sliding groove, the clamping seat is in sliding connection with the fixing seat up and down, the clamping seat is in welded connection with the spring, the avoiding hole is positioned at the left side top inside the clamping seat, the avoiding hole is a circular through hole, the lower bottle body is positioned at the upper end inside the clamping sleeve, the lower bottle body is movably connected with the clamping sleeve, the lower centrifugal rotary drum is fixedly arranged inside the lower bottle body, the utility model provides a bottle body, including lower centrifugal rotary drum, lower bottle body, upper centrifugal rotary drum, filter layer, lower centrifugal rotary drum, the filter layer set firmly in lower centrifugal rotary drum inside middle-end, filter layer and lower centrifugal rotary drum adopt medical grade adhesive to be connected, last bottle be located lower bottle upper end, last bottle and lower bottle adopt bayonet connection, last centrifugal rotary drum set firmly in last bottle body inside, last centrifugal rotary drum and last bottle body hot melt connection, rubber buffer quantity be two, the rubber buffer set firmly respectively in the port department of lower bottle and last bottle, the rubber buffer set firmly respectively with lower bottle and last bottle body hot melt connection.
2. The device for separating and purifying tumor cells in malignant hydrothorax and ascites as claimed in claim 1, wherein a handle is further fixedly arranged on the right side of the fixing seat, the handle and the fixing seat are integrally formed, a storage battery is further fixedly arranged at the lower end of the right side inside the fixing seat, the storage battery is connected with the fixing seat through bolts, a charging interface is further fixedly arranged on the right side of the storage battery, the charging interface is connected with the fixing seat through bolts, and the charging interface is connected with the storage battery through electric wires.
3. The device for separating and purifying tumor cells in malignant hydrothorax and ascites as claimed in claim 1, wherein the control panel comprises a PLC processor, an A/D converter, a display screen, a servo driver and a delay relay, and the servo driver and the delay relay are respectively connected with a servo motor by signal wires.
4. The device for separating and purifying tumor cells in malignant hydrothorax and ascites as claimed in claim 1, wherein a pulling handle is further fixedly arranged on the right side of the top of the clamping seat, and the pulling handle and the clamping seat are integrally formed.
5. The device for separating and purifying tumor cells in malignant hydrothorax and ascites as claimed in claim 1, wherein a clamping groove is further arranged in the clamping sleeve, the clamping groove is a triangular groove, and the clamping groove is connected with the lower bottle body in an up-down sliding manner.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911283759.4A CN112972783B (en) | 2019-12-13 | 2019-12-13 | Device for separating and purifying tumor cells in malignant hydrothorax and ascites |
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| CN112972783B true CN112972783B (en) | 2024-04-05 |
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| CN120284333B (en) * | 2025-06-09 | 2025-10-17 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | Body fluid sample collection device for hydrothorax and ascites and application method thereof |
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| CN204779578U (en) * | 2015-06-08 | 2015-11-18 | 沈阳市第四人民医院 | Draw drop filter equipment of cell of chest ascites |
| CN104962520A (en) * | 2015-06-12 | 2015-10-07 | 广州医科大学附属第一医院 | Method for fast and efficient separation and purification of tumor cells from malignant pleural effusion and ascites |
| CN107432958A (en) * | 2017-08-13 | 2017-12-05 | 李凯 | Wet lung drainage arrangement |
| CN209589868U (en) * | 2019-03-06 | 2019-11-05 | 成都中珠健联基因科技有限责任公司 | The extraction element of high efficiency extraction tumour cell |
| CN110192914A (en) * | 2019-06-26 | 2019-09-03 | 厦门市第五医院(厦门市同民医院) | A kind of tumor operation hydrops extractor tool |
| CN110257241A (en) * | 2019-07-04 | 2019-09-20 | 北京晶科瑞医学检验实验室有限公司 | A kind of device of the quick separating tumour cell from lung's malignant hydrothorax |
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