CN113413402B - Application of Plum Blossom Extract in the Preparation of Medicines for Helicobacter pylori Infection - Google Patents

Abstract

The invention discloses the application of Plum Blossom extract in preparing medicines for treating Helicobacter pylori infection, belonging to the technical field of medicine. The invention determines for the first time that Plum Blossom extract can inhibit the virulence and colonization ability of H. Experiments have verified that the extract of Prunus chinensis can effectively inhibit the infection and inflammatory state of H. pylori. The effect of its single medication is better than or equal to the therapeutic effect of triple or quadruple combination. It can be used in combination with triple or quadruple combination to synergistically improve the treatment of H. pylori infection. After the eradication effect and gastric mucosa protection effect; the use of Prunus chinensis extract for the treatment of H. pylori infection has low cost, short course of treatment and convenient treatment; the present invention provides a new idea for the treatment of H. pylori infection, and is popularized for the clinical treatment of H. pylori has great significance.

Description

Application of Meihuacao Extract in the Preparation of Drugs for Treating Helicobacter Pylori Infection Diseases

technical field

The invention belongs to the technical field of medicines, and in particular relates to the application of an extract of Prunus chinensis in the preparation of medicines for treating Helicobacter pylori infection diseases.

Background technique

Prunus palustris (Parnassis palustris L.) is the whole herb or root of the saxifrage plant Prunus genus, also known as white side grass, horseshoe grass, etc. For Namu Cannes, Uldige and Namu Riyinchagan-Qiqige and so on. It is one of the commonly used Mongolian medicinal materials for the treatment of tumors and related diseases in the clinical application of modern Mongolian medicine. Plum blossom grass smells sweet and bitter, without obvious toxicity, and has the functions of clearing away heat and detoxifying, dispelling swelling, reducing swelling and discharging pus, and suppressing sunburn. , gallbladder tumors and some intestinal tumors (such as colon tumors), etc., can also treat jaundice hepatitis, pharyngitis, mumps, vasculitis and tuberculosis.

Helicobacter pylori (Hp) is a microaerophilic Gram-negative bacillus widely colonized in the human stomach. More than 50% of people in the world are infected by it. Studies have confirmed that Helicobacter pylori infection is closely related to the high incidence of chronic gastritis, gastric ulcer, duodenal ulcer and gastric cancer. At present, the main methods for the clinical treatment of Helicobacter pylori include triple or quadruple therapy, that is, 2 or 3 antibiotics combined with 1 proton pump inhibitor or bismuth. Due to the particularity of the gastric environment and the emptying of the stomach, the treatment of Helicobacter pylori requires a large amount of medication, a long course of treatment, high costs, and large side effects, resulting in poor patient compliance. Some patients stop taking medicine before reaching the complete course of treatment. In addition, Helicobacter pylori It is easy to produce drug resistance to the antibiotics used, and even drug-resistant strains to 6 kinds of antibiotics have appeared, and Helicobacter pylori is also easy to re-infect. Therefore, eradicating Helicobacter pylori infection has become a medical problem. Although traditional Chinese medicine compound preparations have good curative effects in treating Helicobacter pylori, their ingredients are unclear, the course of treatment is long, it is inconvenient to take, and the cost is higher than that of western medicines.

At present, there is no relevant report on the preparation of medicines for treating diseases infected by Helicobacter pylori with the extract of Prunus chinensis.

Contents of the invention

In order to solve the above-mentioned problems in the prior art, the present invention provides the application of the extract of Prunus chinensis in the preparation of medicines for treating Helicobacter pylori infection.

To achieve the above object, the present invention provides the following technical solutions:

The invention provides the application of the extract of Prunus chinensis in the preparation of medicine for treating Helicobacter pylori infection.

Further, the Helicobacter pylori infection disease is gastritis, gastric ulcer, duodenal ulcer or gastric cancer.

Further, the preparation method of the quince extract comprises the following steps:

Preparation of the organic phase extract of Prunus chinensis: heating and reflux extracting the Prunus chinensis with ethanol to obtain an extract, then removing the ethanol in the extract, and drying to obtain the organic phase extract of Prunus chinense;

Preparation of the water-phase extract of Prunus chinensis: heating and leaching Prunus chinensis with water, filtering to obtain the extract, concentrating, drying the obtained concentrated solution, and obtaining the water-phase extract of Prunus chinense;

The Herba Prunus organic phase extract and the Herba Prunus aqueous phase extract are mixed in equal mass to obtain the Herba Prunus officinalis extract.

Further, in the preparation process of the organic phase extract of Prunus chinensis, the ethanol is 95vol.% ethanol, and during the heating and reflux extraction, the solid-liquid ratio of Plum blossom and ethanol is 1g: (1-5mL), and the heating temperature The temperature is 90-95°C, each extraction is 4h, a total of 3 extractions are made, and the extracts obtained from the 3 extractions are combined, and the drying is spray drying.

Further, the spray drying is carried out in a spray drying tower, the air inlet temperature of the spray drying tower is 120°C-150°C, the air outlet temperature is 90°C-95°C, the atomization frequency is 220-300Hz, and the feeding frequency is 4-300Hz. 6.5Hz, the induced wind frequency is 20~50Hz.

Spray-drying the ethanol extract of Meihuacao can better retain the inherent aromatic substances and heat-sensitive active ingredients of Meihuacao.

Further, in the preparation process of the aqueous phase extract of Prunus chinensis, when water is used to heat and extract Plum blossoms, the ratio of solid to water is 1g: (5-10mL), and the heating temperature is 90-95°C. The leaching time is 1-2h, the concentration is specifically concentrated under reduced pressure at 50-60°C, the drying temperature is 65-75°C, and distilled water is preferably used to heat and extract Prunus chinensis.

Further, the Prunus quince extract is added as an effective active ingredient to pharmaceutically acceptable excipients to make tablets, capsules, granules, oral liquids, suspensions or injections, and the quality of the Prunus quince extract in various dosage forms It accounts for 30-60% of the total mass of the drug.

Compared with the prior art, the present invention has the following beneficial effects:

The present invention confirms for the first time that the extract of Prunus chinensis can inhibit the virulence and colonization ability of H. It is equal to or equivalent to the therapeutic effect of the triple combination and the quadruple combination, and its combined use with the triple combination or the quadruple combination can synergistically improve the therapeutic effect on Hp infection;

Use the extract of Plum Blossom Grass to treat Hp infection, which has low cost, short course of treatment and convenient treatment;

The invention provides a new idea for the treatment of Hp infection diseases, and has great significance for the clinical treatment of Hp.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the embodiments. Obviously, the accompanying drawings in the following description are only some of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without any creative effort.

Fig. 1 is the statistical graph of the relative expression of Hp-related virulence and colonization genes (Cag A, Vac A, Bab A) in the Hp group and the Prunella treatment group;

Figure 2(a) and Figure 2(b) are the general appearance and morphology of the gastric mucosa of mice in the Hp infection group and the Prunus pylori treatment group infected with Hp, respectively;

Figure 3 is the HE staining diagram of the gastric mucosa of mice in each group;

Fig. 4 is the transmission electron micrograph of the mitochondrial structure of the gastric mucosa of each group of mice;

Fig. 5 is a transmission electron microscope image of gastric mucosal microvilli of mice in each group.

Detailed ways

Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features and embodiments of the present invention. It should be understood that the terminology described in the present invention is only used to describe specific embodiments, and is not used to limit the present invention.

In addition, regarding the numerical ranges in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only the preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and/or materials in connection with which the documents are described. In case of conflict with any incorporated document, the contents of this specification control.

It will be apparent to those skilled in the art that various modifications and changes can be made in the specific embodiments of the present invention described herein without departing from the scope or spirit of the present invention. Other embodiments will be apparent to the skilled person from the description of the present invention. The description and examples of the invention are illustrative only.

As used herein, "comprising", "comprising", "having", "comprising" and so on are all open terms, meaning including but not limited to.

The plum blossoms used in the following examples were collected from the Saihan Ula National Nature Reserve in Chifeng City, Inner Mongolia Autonomous Region. After collection, the whole plant was placed indoors to dry naturally in the shade. The professor jointly identified it as Plum Grass (ParnassiapalustrisLinn).

The Helicobacter pylori standard strain SS1 that the present invention adopts is preserved and donated by the Gastroenterology Laboratory of Peking University First Hospital; the male Kunming rat (KM rat) adopted is 6 weeks old, and the body weight is between 36-41g, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., its experimental animal production license: Beijing Baishan SCXK (Beijing) 2016-0006; experimental animal use license: Beijing SYXK (Beijing) 2017-0033; the triple drug used: Rabeprazole Sodium Enteric-coated Tablets: Eisai Pharmaceuticals (Specification 10mg*7); Clarithromycin Tablets: Shanghai Abbott (Specification 250mg*8); Amoxicillin Capsules: Zhuhai Federation (Specification 500mg*48/24), The quadruple drugs used are based on the above triple drugs, plus colloidal pectin bismuth capsules: Shanxi Ante (specification 100mg*48);

The description will not be repeated below.

Example 1

The preparation of Prunus japonica extract comprises the following steps:

(1) Extraction of the organic phase of Prunus chinensis: take 0.40 kg of the whole dried Plum Blossom grass, use 400 ml of 95vol.% ethanol to heat to 90°C for reflux extraction 3 times, each time for 4 hours, and combine the extracts obtained by 3 extractions , after volatilizing the ethanol, the ethanol extract of Prunus chinensis is obtained; the above-mentioned extract is dried in a spray drying tower to obtain the organic phase extract of Prunus chinensis, the air inlet temperature of the spray drying tower is 120°C, and the outlet air temperature is 90°C, The atomization frequency is 220Hz, the feeding frequency is 4Hz, and the air induction frequency is 20Hz, which can better retain the inherent aromatic substances and heat-sensitive active ingredients of Meihua grass.

(2) Aqueous phase extraction of plum blossom grass: Weigh 25 grams of dried plum blossom grass, pulverize it with a powder machine, inject 200ml of deionized water, extract it in a water bath at 90°C for 1 hour, filter it, and concentrate it under reduced pressure at 60°C after filtration. liquid, use a 0.22um filter to sterilize the obtained aqueous phase extract, dry the filtered concentrated solution in an oven at 75°C to obtain the aqueous phase extract of Prunus chinensis, and set aside;

(3) Mixing the organic phase extract of Herba Prunus obtained in step (1) with the aqueous phase extract of Herba Prunus obtained in step (2) to obtain the Herba Prunus extract.

Example 2

Preparation of Prunus officinalis extract:

(1) Extraction of the organic phase of Prunus chinensis: take 0.40 kg of the whole dried Plum chinensis, use 2000 ml of 95vol.% ethanol to heat to 95°C for reflux extraction 3 times, each time for 4 hours, and combine the extracts obtained by 3 extractions , after volatilizing ethanol, the ethanol extract of Prunus chinensis is obtained; the above-mentioned extract is dried in a spray drying tower to obtain the organic phase extract of Prunus chinensis, the air inlet temperature of the spray drying tower is 150°C, and the outlet air temperature is 95°C, The atomization frequency is 300 Hz, the feeding frequency is 5 Hz, and the air induction frequency is 30 Hz, which can better retain the inherent aromatic substances and heat-sensitive active ingredients of Meihua grass.

(2) Aqueous phase extraction of plum blossom grass: Weigh 25 grams of dried plum blossom grass, pulverize it with a powder machine, inject 125ml of deionized water, extract it in a water bath at 95°C for 1 hour, filter it, and concentrate it under reduced pressure at 50°C after filtration. liquid, use a 0.22um filter to sterilize the obtained aqueous phase extract, dry the filtered concentrated solution in an oven at 65°C to obtain the aqueous phase extract of Prunus chinensis, and set aside;

(3) Mixing the organic phase extract of Herba Prunus obtained in step (1) with the aqueous phase extract of Herba Prunus obtained in step (2) to obtain the Herba Prunus extract.

Example 3

Preparation of Prunus officinalis extract:

(1) Extraction of the organic phase of Prunus chinensis: take 0.40 kg of the whole dried Plum chinensis, use 1000 ml of 95vol.% ethanol to heat to 90 ° C for reflux extraction for 3 times, each time for 4 hours, and combine the extracts obtained from the 3 extractions , after volatilizing ethanol, the ethanol extract of Prunus chinensis is obtained; the above-mentioned extract is dried in a spray drying tower to obtain the organic phase extract of Prunus chinensis, the air inlet temperature of the spray drying tower is 130°C, and the air outlet temperature is 90°C, The atomization frequency is 250Hz, the feeding frequency is 6.5Hz, and the air induction frequency is 50Hz, which can better retain the inherent aromatic substances and heat-sensitive active ingredients of Plum Blossom Grass.

(2) Water-phase extraction of plum blossom grass: Weigh 25 grams of dried plum blossom grass, pulverize it with a powder machine, inject 250ml of deionized water, extract it in a water bath at 90°C for 2 hours, filter it, and concentrate it under reduced pressure at 55°C after filtration to obtain concentrated liquid, use a 0.22um filter to sterilize the obtained aqueous phase extract, dry the filtered concentrated solution in an oven at 70°C to obtain the aqueous phase extract of Prunus chinensis, and set aside;

(3) Mixing the organic phase extract of Herba Prunus obtained in step (1) with the aqueous phase extract of Herba Prunus obtained in step (2) to obtain the Herba Prunus extract.

Effect verification example 1

Determination of the minimum inhibitory concentration (minimal inhibitory concentration, MIC) of Prunus alba extract by agar dilution method

The agar dilution method was used to determine the MIC of the extract of Prunus chinensis, specifically as follows: Hp cultured for 48-72 hours was scraped and inoculated in liquid medium, and diluted to 1.5*10 ⁸ CFU/mL with sterile saline. Add 200uL of bacterial solution dropwise on the surface of the culture medium, and inoculate evenly with different final concentrations of Prunus officinalis extracts (40g/L, 20g/L, 10g/L, 5g/L, 2.5g/L, 1.25g/ L) on the drug-containing plate, observe after 72 hours of microaerophilic culture at 37°C, and take the lowest drug concentration at which no Hp growth occurs as the MIC. The results of MIC determination were judged and interpreted according to the National Committee for Clinical Laboratory Standards (NCCLS).

The agar dilution method was used to determine the MIC, and it was found that no Hp appeared in the 20g/L and 10g/L Prunus chinensis drug-containing plates, that is, 10g/L was the MIC value. The results suggested that the extract of Prunus japonica had certain anti-Hp activity.

Effect verification example 2

Effect of real-time quantitative PCR on the expression of genes related to Hp colonization and adhesion

Hp infection first expresses Bab A and other outer membrane proteins to adhere to gastric mucosal cells, and then induces inflammation and cell damage by expressing VacA and Cag A proteins, thereby increasing the permeability of gastric mucosa and facilitating its migration. Bab A is the Hp outer membrane protein gene, which can specifically bind to host cell receptors and endow Hp with persistent colonization ability; highly virulent Hp strains have a cytotoxin-related gene (Cag A), which is involved in Cag A transport and host inflammatory response. Vascular cytotoxin A (Vac A) can destroy cell endocytosis, inhibit immune cells and lead to immune tolerance and chronic infection, so the primer design of the above-mentioned related genes is carried out, see Table 1, and the toxicity of Prunella herba extract to Hp and colonization effects.

Dissolve Prunus chinensis extract in distilled water to a final concentration of 20mg/ml. Resuspend the cultured Hp bacterial solution to 1*10 ⁷ CFU/ml with PBS, add 0.1ml of Hp suspension in PBS to the prepared Prunus chinensis extract solution, incubate at 37°C with shaking and micro-aerobic for 24 hours, centrifuge and discard The supernatant was washed 3 times with PBS, and the total RNA was isolated using RNA prepare Cell/Bacteria Kit (Tiangen Company, China). The cDNA was synthesized according to the operation instructions of the kit (SYBR@primeScript ^TM RT-PCR Kit). Primer sequences are shown in Table 1. The PCR reaction system is: cDNA 2ul, UltraSYBR mixture 25ul, 10uM upstream and downstream primers 1ul each, made up to 50ul with deionized water. Reaction conditions, 95°C, 10min, 95°C, 10s, 60°C, 1min, 40 cycles, using 2- ^△△CT method to calculate the relative expression of the target gene. And take the Hp bacteria liquid without adding the Prunus quinquefolium extract solution as the control group (the treatment method is the same as above), and compare the relative expression of the target gene in the two groups.

Table 1

Using 16S rRNA gene as an internal reference, 2- ^△△CT was used to analyze the differences in the expression of Hp colonization-related genes in the control group and after the treatment of Prunus chinensis extract. The results are shown in Figure 1. It can be seen from Figure 1 that the extract of Prunus chinensis can inhibit the expression of the above-mentioned virulence genes of BabA, Cag A and Vac A. Therefore, it is speculated that Prunus chinensis can reduce the effect of Hp on the gastric mucosa by inhibiting the expression of Hp-related colonization and virulence genes. colonization and damage.

Effect verification example 3

Animal modeling and group administration observation

Suspension preparation of Helicobacter pylori: adopt microaerophilic culture method, gas reaches microaerophilic condition (85% nitrogen, 10% carbon dioxide, 5% oxygen) under the microaerophilic environment, inoculate Helicobacter pylori bacterial strain in containing 7 % on the horse serum solid plate, every 100ml of solid Hp medium (Hp solid medium: Merck Campylobacter jejuni medium 20g, add 500ml of distilled water to dissolve, 6.81kg autoclave for 15min, cool to about 50 ℃ and import 50ml of sheep blood and Add 1 ml of microbial additives to the antibiotic selection solution (mixed and poured into a sterile culture plate), and incubate at a constant temperature of 37° C. for 48 hours. After being identified as positive by rapid urease, oxidase, smear Gram staining and catalase, the bacteria were collected with an inoculation loop, mixed evenly in the bacterial transfer base (bacterial transfer base: 6.75g of bovine heart and brain infusion was dissolved in 500ml of distilled water , 6.81kg was autoclaved for 15 minutes, cooled to 4°C and dispensed into equipment), and the concentration of the turbidimetric tube was controlled at 1*10 ⁹ CFU/ml for later use.

Experimental method: 132 KM mice were randomly divided into 8 groups, including 12 healthy groups (hereinafter also referred to as "normal group") without treatment at all, 12 normal saline groups (Saline group), 18 Hp groups, 18 in the triple group, 18 in the quadruple group, 18 in the plum blossom group, 18 in the "triple + plum blossom group", and 18 in the "quadruple + plum blossom group". group, Plum Blossom Grass group, "triple + Plum Blossom Grass group" and "quadruple + Plum Blossom Grass group" were intragastrically treated with Helicobacter pylori suspension, the amount of suspension was 400 μL/mouse, and then gavaged once every other day. 200 μL/mouse each time, after 4 weeks, 3 mice were selected from each group to evaluate the infection status of Hp. The untreated healthy group was given no drug, the normal saline group was only given normal saline, the Hp group was only given normal saline at the later stage, the triple combination group was only given triple medicine, the quadruple medicine group was only given quadruple medicine, and the Meihuacao group was only given Meihuacao extract. Herb extract and triple drug were administered at the same time in the "triple combination + herb group", and the herb extract and quadruple drug were given at the same time in the "quadruple + herb group". Among them, the dosage of herb extract: according to the standard Adult Plum Blossom Grass Extract 2000mg bid, the dosage is converted according to the body weight of Kunming rats. The dosage of triple drugs: specifically refer to the clinical standard adult dosage for conversion, the dosage of 70kg adult triple method (rabeprazole 20mg bid, amoxicillin 1000mg bid, clarithromycin 500mg bid). Quadruple drug dosage: based on adult quadruple therapy (rabeprazole 20mg bid, amoxicillin 1000mg bid, clarithromycin 500mg bid + colloidal bismuth pectin 300mg bid) , according to the body weight conversion of standard adults (70kg) and KM mice.

The mice in each group were dosed one day apart, once a day, and each time the drug was administered, the drug was stirred in water to obtain 200 μL of the drug solution, which was intragastrically administered after fasting for 12 hours. Fasted with water for half an hour after the drug, administered 5 times in a row, then sacrificed KM mice, collected serum and dissected gastric tissue for relevant experimental detection, carried out rapid urease test (RUT) and immunohistochemical staining of HE and Hp, and analyzed Oxidative stress indicators in serum were detected by ELISA and analyzed by transmission electron microscope on gastric mucosal tissue.

Observe the gastric mucosa of mice in Hp group and Meihuacao group, as shown in Fig. However, the surface of the gastric mucosa in the Prunella extract group was smooth, and no erosion, hemorrhage, or ulcer was seen.

HE staining and observation (100X) were performed on the gastric mucosa of mice in each group, as shown in Figure 3: the structure of the gastric mucosa in the normal group was clear, while in the Hp infection group, mucosal exfoliation, erosion, and thinning of the mucosal layer were seen. The triple and quadruple groups can have a certain protective effect. The plum flower group can significantly increase the thickness of the gastric mucosa, protect and promote the repair of the gastric mucosa.

The rapid urease method (RUT) was used to detect the Helicobacter pylori infection in the gastric mucosa of mice in each group, and the results are shown in Table 2.

Table 2

As can be seen from Table 2, the extract of Prunus chinensis has a good therapeutic effect on Helicobacter pylori infection. The combination of grass extract and triple or quadruple can effectively improve the cure rate.

The mitochondria and gastric mucosal microvilli of the gastric mucosal cells of the mice in each group were observed by transmission electron microscopy, and the results are shown in Figure 4 and Figure 5, respectively. As shown in Figure 4: In the normal group, the mitochondrial structure is complete and the inner cristae are clear. After Hp infection, the mitochondrial structure is broken and the inner crest is disordered. The triple and quadruple groups can repair the structure of the mitochondria to a certain extent. The structure has a protective effect. The combination of triple and quadruple extracts can effectively protect mitochondrial function. The results in Figure 4 suggest that single or combined extracts of plum blossoms can protect the mitochondrial structure of gastric mucosal epithelial cells after Hp infection. As shown in Figure 5: in the normal group, the microvilli were arranged neatly and had a longer structure. However, after Hp infection, the microvilli were found to be broken and disappeared, and after triple or quadruple therapy, they all recovered to varying degrees. A single quince extract can also better restore the microvilli structure. When the quince extract is combined with triple or quadruple, the overall microvilli structure is better. The results in Fig. 5 suggest that the extract of Prunus chinense alone or in combination with triple or quadruple can protect the microvilli structure of gastric mucosal epithelial cells after Hp infection.

The enzyme-linked immunosorbent assay (ELISA) was used to detect serum IL-2, IL-8 and TNF-a of mice in each group, and the results are shown in Table 3, unit: pg/mg.

table 3

Note: ^* : p<0.05, ^** : p<0.01.

It can be seen from Table 3: through serum ELISA detection, the expression levels of IL-2, IL-8 and TNF-a were significantly increased in the Hp group, while the levels of related inflammatory factors were significantly decreased in the group treated with Prunella herba extract, Compared with the simple Hp infection group, there is a statistical difference (p<0.05), and the anti-inflammatory and anti-oxidative effects of the Prunella herba extract combined with triple and quadruple treatment groups are more obvious.

Carry out the above-mentioned effect verification according to the method of above-mentioned effect verification example 1, effect verification example 2 and effect verification example 3 to the Prunus japonica extract prepared by embodiment 2 and embodiment 3, the result obtained is the same as that of the Prunus japonica extract in embodiment 1. The effect is the same.

The above is only a preferred specific embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Anyone familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solutions of the present invention and Any equivalent replacement or change of the inventive concepts shall fall within the protection scope of the present invention.

Claims (3)

1. The application of Prunus alba extract as an effective component in the preparation of medicines for treating Helicobacter pylori infection; The preparation method of described plum blossom extract, comprises the following steps: The preparation of the organic phase extract of Prunus officinalis: use 95vol.% ethanol to heat and reflux the Prunus officinalis to extract the extract, then remove the ethanol in the extract, and spray dry to obtain the organic phase extract of Prunus officinalis; wherein, Prunus officinalis and The solid-liquid ratio of ethanol is 1g: (1-5mL), the heating temperature is 90-95°C, each extraction is 4h, a total of 3 extractions are made, and the extracts obtained from the 3 extractions are combined; The preparation of the water-phase extract of Prunus chinensis: heating and leaching Plum blossom with water, filtering to obtain the extract, then concentrating, and drying the obtained concentrated solution to obtain the aqueous phase extract of Prunus quince; The ratio of solid to liquid is 1g: (5-10mL), the heating temperature is 90-95°C, the extraction time is 1-2h, the concentration is specifically concentrated under reduced pressure at 50-60°C, and the drying temperature is 65- 75°C; The Herba Prunus organic phase extract and the Herba Prunus aqueous phase extract are mixed in equal mass to obtain the Herba Prunus officinalis extract. 2. The application according to claim 1, characterized in that the Helicobacter pylori infection disease is gastritis, gastric ulcer, duodenal ulcer or gastric cancer. 3. application according to claim 1, is characterized in that, described plum blossom extract is added pharmaceutically acceptable adjuvant as effective component to make tablet, capsule, granule, oral liquid, suspension or injection Liquid, the quality of the plum blossom extract in various dosage forms accounts for 30-60% of the total medicine mass.

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