CN118258676A - Sample analyzer and reagent configuration method - Google Patents

Sample analyzer and reagent configuration method Download PDF

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Publication number
CN118258676A
CN118258676A CN202211675850.2A CN202211675850A CN118258676A CN 118258676 A CN118258676 A CN 118258676A CN 202211675850 A CN202211675850 A CN 202211675850A CN 118258676 A CN118258676 A CN 118258676A
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China
Prior art keywords
reagent
container
basic
perform
dispensing
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CN202211675850.2A
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Chinese (zh)
Inventor
方振
张勇
李江波
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Priority to CN202211675850.2A priority Critical patent/CN118258676A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The embodiment of the application provides a sample analyzer and a reagent configuration method, wherein the reagent configuration method is applied to the sample analyzer and comprises the following steps: controlling a reagent dispensing mechanism of the sample analyzer to perform a first reagent configuration operation for configuring the first reagent during a reagent dispensing cycle of the sample analyzer; after the first reagent configuration operation is completed, controlling the reagent dispensing mechanism to execute a target reagent configuration operation so as to mix the first reagent and a third basic reagent to form a target reagent, wherein the first reagent is a PCR reagent, and the third basic reagent is an IC reagent. The technical scheme provided by the embodiment of the application can reduce the sample adding error of the reagent and improve the accuracy and reliability of the detection result of the detection sample.

Description

Sample analyzer and reagent configuration method
Technical Field
The application relates to the technical field of medical instruments, in particular to a sample analyzer and a reagent configuration method.
Background
Nucleic acid is a basic object of molecular biology research, in molecular diagnosis, a plurality of reagents are generally used to extract nucleic acid substances such as DNA or RNA from a biological sample, and the extracted nucleic acid substances are detected to obtain corresponding detection results, so that medical staff can use the obtained detection results as important basis of diagnosis and treatment or related research, and therefore, the accuracy of the detection results is very important.
However, the accuracy of the detection result is affected by various factors, wherein one of the more critical factors is the accuracy of adding the corresponding reagent in the sample processing process, for example, in the process of performing molecular diagnosis on the sample to be detected by the sample analyzer, MIX reagent, enzyme reagent and IC (internal control) reagent are needed, and the MIX reagent and enzyme reagent have high requirements on the accuracy and repeatability of the sample addition, and if the sample addition of the reagent has errors, the accuracy and the repeatability of the amplification result of PCR (Polymerase Chain Reaction ) are poor, thereby affecting the accuracy and the accuracy of the detection result. For example, since the amount of the IC reagent to be added is small, usually in microliters, a small amount of reagent to be added easily introduces errors in the accuracy of reagent addition, and thus affects the accuracy and reliability of the detection result.
Therefore, how to reduce the error of the sample adding precision of the reagent to improve the precision and reliability of the detection result of the detection sample is a popular subject for those skilled in the art.
Disclosure of Invention
The embodiment of the application mainly aims to provide a sample analyzer and a reagent configuration method, aiming at reducing reagent sample adding errors and improving the accuracy and reliability of detection results of detection samples.
In a first aspect, embodiments of the present application provide a sample analyzer, comprising:
A reagent supply mechanism provided with a reagent placement site for placing a reagent container, the reagent placement site including a first reagent placement site for placing a first reagent container, a second reagent placement site for placing a second reagent container, and a third reagent placement site for placing a third reagent container, wherein the first reagent container stores a first base reagent for configuring the first reagent, the second reagent container stores a second base reagent for configuring the first reagent, and the third reagent container stores a third base reagent;
a reagent dispensing mechanism for performing a reagent dispensing operation at least during a reagent dispensing cycle to dispense reagent to a corresponding reagent container; and
A controller for at least:
controlling the reagent dispensing mechanism to perform a first reagent dispensing operation during the reagent dispensing cycle, the first reagent dispensing operation for dispensing the first reagent;
After the execution of the first reagent preparing operation is completed, controlling the reagent dispensing mechanism to execute a target reagent preparing operation so as to prepare a target reagent by using the first reagent and the third basic reagent, wherein the first reagent is a PCR reagent, and the third basic reagent is an IC reagent.
In a second aspect, an embodiment of the present application provides a reagent configuration method, applied to a sample analyzer, including:
Controlling a reagent dispensing mechanism of the sample analyzer to perform a first reagent configuration operation for configuring the first reagent during a reagent dispensing cycle of the sample analyzer;
After the execution of the first reagent preparing operation is completed, controlling the reagent dispensing mechanism to execute a target reagent preparing operation so as to prepare a target reagent by using the first reagent and the third basic reagent, wherein the first reagent is a PCR reagent, and the third basic reagent is an IC reagent.
In a third aspect, embodiments of the present application provide a sample analyzer, comprising:
A reagent supply mechanism provided with a plurality of reagent placement sites for placing reagent containers for placing base reagents;
the information acquisition mechanism is used for acquiring reagent information corresponding to the reagent stored in the reagent container in the reagent storage position;
a reagent dispensing mechanism for performing a reagent dispensing operation;
a reagent dispensing mechanism for performing a reagent dispensing operation at least during a reagent dispensing cycle to dispense reagent to a corresponding reagent container; and
A controller for at least:
Controlling the information acquisition mechanism to acquire reagent information corresponding to the reagent stored in the reagent container in the reagent placement position, and determining a reagent configuration strategy according to the information of the item to be detected of the sample analyzer and the reagent information;
Controlling the reagent dispensing mechanism to perform a reagent dispensing operation according to the reagent configuration strategy so as to dispense a base reagent for configuring a reagent to be configured;
And controlling the reagent dispensing mechanism to execute reagent dispensing operation according to the reagent dispensing strategy in the reagent dispensing cycle so as to dispense the reagent to be dispensed by using the dispensed basic reagent.
The embodiment of the application provides a sample analyzer and a reagent configuration method, wherein the sample analyzer comprises: a reagent supply mechanism provided with a reagent placement site for placing a reagent container, the reagent placement site including a first reagent placement site for placing a first reagent container, a second reagent placement site for placing a second reagent container, and a third reagent placement site for placing a third reagent container, wherein the first reagent container stores a first base reagent for configuring the first reagent, the second reagent container stores a second base reagent for configuring the first reagent, and the third reagent container stores a third base reagent; a reagent dispensing mechanism for performing a reagent dispensing operation at least during a reagent dispensing cycle to dispense reagent to a corresponding reagent container; and a controller for at least: controlling the reagent dispensing mechanism to perform a first reagent dispensing operation during the reagent dispensing cycle, the first reagent dispensing operation for dispensing the first reagent; after the execution of the first reagent preparing operation is completed, controlling the reagent dispensing mechanism to execute a target reagent preparing operation so as to prepare a target reagent by using the first reagent and the third basic reagent, wherein the first reagent is a PCR reagent, and the third basic reagent is an IC reagent. In the embodiment of the application, the corresponding reagents are mixed to achieve the effect of reagent dilution. Reagent sample adding is carried out by utilizing diluted reagent, and the sample adding amount of the diluted reagent is larger than the reagent sample adding amount of the reagent before dilution, so that the influence of low sample adding amount precision errors on a detection result is effectively solved, the reagent sample adding error is reduced, and the accuracy and reliability of the detection result of a detection sample are improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic block diagram of a sample analyzer according to an embodiment of the present application;
FIG. 2 is a schematic diagram showing the configuration of the cooperation of a reagent supply mechanism and an information acquisition mechanism of a reagent processing apparatus in a sample analyzer;
FIG. 3 is a schematic view showing the structure of a reagent dispensing mechanism of the reagent processing apparatus;
FIG. 4 is a schematic illustration of a scenario in which a target reagent is configured and used;
FIG. 5 is a schematic view showing a reagent dispensing mechanism of the reagent processing apparatus for dispensing a reagent into a reagent container;
FIG. 6 is a schematic view showing a case where a reagent dispensing mechanism of the reagent processing apparatus performs a mixing operation on a reagent in a reagent container;
FIG. 7 is a flow chart of steps of a reagent configuration method according to an embodiment of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
In the description of the present application, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
Some embodiments of the present application will be described in detail below with reference to the attached drawings, and the following examples and features of the examples may be combined with each other without conflict.
Referring to fig. 1, fig. 1 is a sample analyzer 100 according to an embodiment of the present application, configured to perform detection analysis on a sample to be detected to obtain a corresponding detection result, where the sample to be detected includes, but is not limited to, a nucleic acid sample and a blood sample.
As shown in fig. 1, the sample analyzer 100 includes a sample introduction device 10, a reagent processing device 20, a separation device 30, a detection device 40, and a controller 50. The reagent processing apparatus 10 is at least used for performing a reagent configuration operation to configure a basic reagent into a corresponding reagent sample injection apparatus 10 for performing a sample injection operation to supply a corresponding sample to be tested to a preset sample injection position. The separation device 30 is used for performing a sample separation operation to separate a reaction liquid formed by mixing at least a sample to be measured and a target reagent, thereby obtaining a sample to be measured. The detecting device 40 is used for performing a sample detecting operation to detect a sample to be tested, so as to obtain detection data.
The controller 50 communicates with the sample injection device 10, the reagent processing device 20, the separation device 30 and the detection device 40, and is used for controlling the corresponding devices to execute preset operation procedures respectively so as to realize respective preset functions. For example, the controller 50 controls the reagent processing apparatus 10 to perform a reagent configuration operation to configure a base reagent into a corresponding reagent. The controller 50 controls the separation device 30 to perform a sample separation operation to separate a reaction liquid formed by mixing at least a sample to be measured and a target reagent, thereby obtaining a sample to be measured.
Alternatively, the controller 50 may be one or more, and may be integrated with or disposed in at least one of the sample injection device 10, the reagent processing device 20, the separation device 30, and the detection device 40, or may be disposed independently, which is not limited herein, and only needs that the controller 50 can control each device of the sample analyzer 100 to execute a corresponding preset operation procedure.
In some embodiments, the sample injection device 10 includes a sample sucking component and an information obtaining component, where the information obtaining component is configured to obtain information of a to-be-measured item corresponding to a to-be-measured sample carried in a sample tube, and the sample sucking component is configured to suck the to-be-measured sample from the sample tube and transfer the to-be-measured sample into a reaction tube.
For example, the sample to be tested is carried on the sample tube, and the corresponding information of the item to be tested of the sample to be tested is set on the outer wall of the sample tube through an information tag, where the information tag may be a two-dimensional code tag, a bar code tag or an RFID (Radio Frequency Identification ) tag, and is not limited herein. In the sample injection process, the information acquisition component scans the information label arranged on the sample tube, so that corresponding information of the item to be detected is obtained. Meanwhile, the sample sucking assembly can perform two-dimensional or three-dimensional movement in space, so that the sample sucking assembly can move to a sample sucking position to suck a sample to be detected loaded in a sample tube positioned at the sample sucking position, and the sample to be detected is discharged into a reaction tube, so that the sample to be detected loaded in the reaction tube reacts with a reagent to form a reaction liquid.
It will be appreciated that in some embodiments, the sample analyzer 100 may not require the sample introduction device 10, and the item information to be measured may be obtained by manually introducing sample through an input device connected to the sample analyzer 100. For example, before or after the sample is introduced, an operator inputs information of a to-be-measured item corresponding to a current to-be-measured sample to the sample analyzer 100 through an input device such as a keyboard, a mouse, a key, a touch panel, etc.
In some embodiments, the reagent processing apparatus 20 includes a reagent supply mechanism 21, an information acquisition mechanism 22, a reagent dispensing mechanism 23, and a reagent dispensing mechanism 24. Wherein the reagent supply mechanism 21 is provided with a reagent placement site 211, the reagent placement site 211 comprising a first reagent placement site 2111 for placing a first reagent container, a second reagent placement site 2112 for placing a second reagent container, and a third reagent placement site 2113 for placing a third reagent container. The first reagent container is used for storing a first basic reagent for configuring a first reagent, the second reagent container is used for storing a second basic reagent for configuring the first reagent, the third reagent container is used for storing a third basic reagent, and the third basic reagent and the first reagent can configure a target reagent.
Optionally, the reagent placement sites 211 further include a fourth reagent placement site 2114 for placing a fourth reagent container for holding a fourth base reagent, and the third base reagent and the fourth base reagent may be configured with a second reagent, and the first reagent and the second reagent may also be configured with a target reagent.
For example, the reagent supply mechanism 21 includes a reagent tray provided with at least a first reagent placement site 2111 for placing a first reagent container, a second reagent placement site 2112 for placing a second reagent container, a third reagent placement site 2113 for placing a third reagent container, and a fourth reagent placement site 2114 for placing a fourth reagent container.
Alternatively, the first basic reagent is a MIX reagent (also referred to as a PCR-MIX reagent), the second basic reagent is a PCR reaction enzyme reagent, the third basic reagent is a first IC reagent, the fourth basic reagent is a diluent, and the fourth basic reagent and the third basic reagent are mixed to obtain a second IC reagent, that is, the diluent is used for diluting the first IC reagent to obtain the second IC reagent in the mixing process of the third basic reagent, that is, both the first IC reagent and the second IC reagent may be referred to as IC reagents, but the reagent concentration of the second IC reagent is smaller than that of the first IC reagent.
Alternatively, the second base reagent is a solid reagent, such as a lyophilized reagent or a powdered reagent. Optionally, the second reagent placement bits 2112 are multiple, and there are multiple second base reagents to be processed placed in the corresponding second reagent containers, and the sample analyzer may dispense the corresponding second base reagents according to the item to be tested of the sample to be tested.
Information acquiring means 22 for acquiring reagent information corresponding to a reagent stored in a corresponding reagent container among the reagent storage positions, for example, the information acquiring means 22 is at least for acquiring reagent information corresponding to a reagent stored in a reagent container placed in at least one of the first reagent storage position 2111, the second reagent storage position 2112, the third reagent storage position 2113, and the fourth reagent storage position 2114.
As shown in fig. 2, a reagent label, which may be a two-dimensional code label, a bar code label, or an RFID label, is attached to the reagent container, the reagent label stores reagent information, and the information acquiring mechanism 22 acquires the reagent information stored in the corresponding reagent container by scanning the reagent label attached to the reagent container, for example.
The sample analyzer 100 is provided with a reagent information collection site at which the information acquisition mechanism 22 performs an information collection operation. Taking as an example the acquisition of reagent information corresponding to a reagent stored in a second reagent container placed in the second reagent site 2112.
In the process of acquiring reagent information corresponding to the reagent stored in the second reagent container, the reagent supply mechanism 21 currently drives the second reagent container to be identified as a target second reagent container to rotate to a reagent information acquisition position, and then the information acquisition mechanism 22 acquires reagent information of a reagent label on the target second reagent container, thereby acquiring a reagent type corresponding to the second basic reagent stored in the target second reagent container.
The reagent dispensing mechanism 23 is at least used for a reagent dispensing operation to dispense a base reagent for dispensing a reagent to be dispensed, and the target reagent is a reagent to be dispensed. Alternatively, the reagent dispensing mechanism 23 is connected to the reagent supplying mechanism 21 for driving the reagent supplying mechanism 21 to rotate in a preset direction to rotate the reagent container on the corresponding reagent placing position in the reagent supplying mechanism 21 as the target reagent container to a preset position. For example, the reagent dispensing mechanism 23 drives the reagent supply mechanism 21 to move the first reagent container placed on the first reagent station 2111 to the reagent dispensing position, so that the reagent dispensing mechanism 24 draws the first base reagent toward the first reagent container at the reagent dispensing position. Or the reagent dispensing mechanism 23 drives the reagent supplying mechanism 21 to move the second reagent container placed at the second reagent position 2112 to the reagent dispensing position so that the reagent dispensing mechanism 24 dispenses the first base reagent into the second base reagent in the second reagent container at the reagent dispensing position, thereby realizing the dispensing of the first reagent.
For another example, the reagent dispensing mechanism 23 drives the reagent supplying mechanism 21 to move the second reagent container placed on the second reagent station 2112 to the reagent information collecting station so that the information acquiring mechanism 22 performs an information collecting operation on the second reagent container at the reagent information collecting station, thereby acquiring reagent information corresponding to the second basic reagent stored in the corresponding second reagent container.
The reagent dispensing mechanism 24 is at least for performing a reagent dispensing operation during a reagent dispensing cycle to dispense reagent to a corresponding reagent container.
The sample analyzer 100 is also provided with a reagent configuration site at which the reagent processing apparatus 20 performs a reagent configuration operation, for example. For example, after determining a target basic reagent to be processed from a plurality of second basic reagents, the controller 50 controls the reagent dispensing mechanism 23 to rotate the reagent supplying mechanism 21 and rotates the second reagent container carrying the target basic reagent to the reagent dispensing position, so that the reagent dispensing mechanism 24 sucks the corresponding first basic reagent from the first reagent container during the reagent dispensing cycle and dispenses the first basic reagent into the corresponding second reagent container, thereby achieving mixing of the first basic reagent and the second basic reagent in the corresponding second reagent container to obtain the first reagent.
Referring to fig. 3, in some embodiments, reagent dispensing mechanism 24 includes a reagent needle 241 and a power assembly 240 for powering reagent needle 241 for performing a reagent dispensing operation. The power assembly 240 includes a first driving mechanism 242 and a first pipetting driving unit 243. The first driving mechanism 242 is used for supporting the reagent needle 241 and driving the reagent needle 241 to move. For example, the reagent needle 241 is spatially moved in two or three dimensions by the two or three-dimensional first driving mechanism 242, so that the reagent needle 241 can be moved to suck up the reagent carried in the corresponding reagent container and discharge the reagent to the corresponding target reagent container.
The first pipetting drive unit 243 is configured to quantitatively aspirate a reagent through the reagent needle 241, for example, the reagent needle 241 is moved under the drive of the first drive mechanism 242 to below the liquid surface of the reagent container carrying the first basic reagent, and quantitatively aspirate the first basic reagent under the drive of the first pipetting drive unit 243, and discharge the aspirated first basic reagent into the second reagent container carrying the second basic reagent, thereby realizing the arrangement of the first reagent.
Optionally, the first driving mechanism 242 includes a supporting frame 2421, the supporting frame 2421 is fixed on the supporting rod 2422, the supporting rod 2422 can move vertically and rotate, and the supporting frame 2421 is driven by the supporting rod 2422 to realize vertical movement and horizontal rotation. The reagent needle 241 is disposed on the supporting frame 2421, and can reach the target position under the driving of the supporting frame 2421. The first driving mechanism 242 further includes a driver 2423 for driving the support bar 2422 to move, for example, a stepper motor, although the present invention is not limited thereto. Optionally, the reagent needle 241 is detachably connected, or fixedly connected, to the first drive mechanism 242.
Optionally, the first pipetting driving unit 243 includes a pipe 2431 and a power unit 2433, where the pipe 2431 is used for transporting a fluid medium, one end of the pipe 2431 is communicated with the reagent needle 241, and the other end is communicated with the power unit 2433, so that the flow direction of the fluid medium in the pipe 2431 is changed under the action of the power unit 2433, so that the reagent needle 241 can transfer a reagent.
In some embodiments, the reagent dispensing mechanism 24 is further configured to dispense a corresponding reagent into the reaction tube carrying the sample to be tested during the reagent dispensing cycle, so that the target reagent reacts with the sample to be tested in the reaction tube to form a sample solution to be tested.
In some embodiments, the separation device 30 employs magnetic separation techniques to extract the components of interest from the reaction solution, thereby obtaining the test sample. The basic principle is that the surface of small particles with magnetic materials is utilized to adsorb required substances through treatment, then the waste liquid is discarded after the adsorption and enrichment of the magnetic materials by a magnet, the enriched magnetic beads are cleaned to further remove impurities and other unnecessary substances, the biological substances of interest are reserved because the biological substances are adsorbed on the magnetic beads, and finally the biological substances enriched on the magnetic beads are released into a required solution system under the preset condition, so that a sample to be tested is obtained.
After the sample to be tested is obtained, the sample to be tested is detected by the detection device 40, so that a corresponding detection result is obtained.
In some embodiments, the reagent processing apparatus 20 further includes a cleaning mechanism for performing a cleaning operation on the target device, e.g., for performing a cleaning operation on the reagent dispensing mechanism 24 that completes the reagent dispensing operation.
In some embodiments, the reagent processing apparatus 20 further includes a mixing mechanism for performing a mixing operation to better mix the solutions in the respective containers.
In some embodiments, the controller 50 includes at least a processor 501, a memory 502, a communication interface (not shown), and an I/O interface (not shown). The processor 501, memory 502, communication interfaces, and I/O interfaces communicate over a bus. The Processor 501 may be a central processing unit (Central Processing Unit, CPU), which may also be other general purpose processors, digital signal processors (DIGITAL SIGNAL Processor, DSP), application SPECIFIC INTEGRATED Circuit (ASIC), field-Programmable gate array (Field-Programmable GATE ARRAY, FPGA) or other Programmable logic device, discrete gate or transistor logic device, discrete hardware components, or the like. Wherein the general purpose processor may be a microprocessor or the processor may be any conventional processor or the like.
The memory 502 contains various computer programs such as an operating system and application programs for execution by the processor 501, and data necessary for execution of the computer programs. During analysis of the sample to be tested, data stored locally may be stored in memory 502, if desired. Meanwhile, the controller 50 invokes the computer program stored in the memory 502 through the processor 501, for controlling the corresponding devices and/or mechanisms in the sample analyzer to respectively execute the preset operation procedures so as to realize the respective preset functions.
The I/O interface includes, but is not limited to, serial interfaces such as USB, IEEE1394, or RS-232C, parallel interfaces such as SCSI, IDE, or IEEE1284, and analog signal interfaces composed of D/a converters, and the like. An input device is coupled to the I/O interface, and a user may directly input data to the controller 50 using the input device, including but not limited to a keyboard, mouse, touch screen, or control buttons. The communication interface may be an interface of any communication protocol known at present, the communication interface communicates with the outside through a network, and the controller 50 may transmit data with any device connected through the network through the communication interface in a preset communication protocol.
It will be appreciated that the controller 50, and in particular the processor 501 of the controller 50, invokes a computer program stored in the memory 502 in implementing the corresponding method steps, thereby implementing the corresponding method steps.
In some embodiments, the controller 50 is configured to implement the following method steps:
controlling the reagent dispensing mechanism 24 to perform a first reagent dispensing operation for dispensing the first reagent during the reagent dispensing cycle;
After the execution of the first reagent preparing operation is completed, the reagent dispensing mechanism 24 is controlled to execute a target reagent preparing operation to prepare a target reagent using the first reagent, which is a PCR reagent, and the third base reagent, which is an IC reagent.
Referring to fig. 2 and 4, the first reagent placement station 2111 of the reagent supply mechanism 21 is illustratively configured to place a first reagent container for holding a first base reagent, and the second reagent placement station 2112 is configured to place a second reagent container in which a second base reagent to be treated is placed.
A third reagent placement station 2113 of the reagent supply mechanism 21 places a third reagent container for holding a third base reagent.
During execution of the first reagent dispensing operation by the reagent dispensing mechanism, the first reagent is formed using the first base reagent and the second base reagent configuration, e.g., the first base reagent is drawn from a container in which the first base reagent is disposed and dispensed into a reagent container of the second base reagent such that the first base reagent and the second base reagent mix to form the first reagent.
After the first reagent configuration is completed, a target reagent is formed using the first reagent and a third base reagent configuration, optionally the first base reagent is a PRC-MIX reagent and the second base reagent is a PCR (Polymerase Chain Reaction ) reactant enzyme reagent. The first basic reagent and the second basic reagent are used for preparing a first reagent, the first reagent is a PCR reagent, and the third basic reagent is an IC reagent. The target reagent is a mixed reagent of a PCR reagent and an IC reagent, and after the target reagent is obtained, the target reagent may be filled into a reaction tube to react the target reagent with a sample in the reaction tube.
Alternatively, the first reagent may be used during a PCR reaction for nucleic acid sample detection and the third base reagent may be used during a nucleic acid extraction reaction.
Based on the reagent configuration process, the basic reagent is utilized to mix so as to realize the dilution of the corresponding reagent to obtain the target reagent, the diluted reagent is utilized to carry out reagent sample loading, and the sample loading amount of the diluted reagent is larger than the reagent sample loading amount of the reagent before dilution, so that the influence of low sample loading amount precision errors on a detection result is effectively solved, the reagent sample loading error is reduced, and the accuracy and reliability of the detection result of a detection sample are improved.
Alternatively, when the sample analyzer 100 needs the reagent processing apparatus 20 to perform the first reagent configuration operation, the controller 50 controls the information acquisition mechanism 22 of the reagent processing apparatus 20 to acquire reagent information corresponding to a reagent stored in the second reagent container in the second reagent storage portion 2112 of the reagent supplying mechanism 21, so as to determine reagent information corresponding to a second basic reagent stored in the reagent supplying mechanism 21 of the current reagent processing apparatus 20, and determines a first reagent required for detecting a sample to be detected according to the reagent information and the item information to be detected of the sample to be detected, and further controls the reagent distributing mechanism 21 to distribute a second basic reagent required for configuring the first reagent.
After the second basic reagent is dispensed, in the reagent dispensing cycle, the reagent dispensing mechanism 24 is controlled to perform a first mixing operation to process the second basic reagent to form the first reagent, so that the second basic reagent can be accurately reconstituted according to the requirement of the item to be tested, and meanwhile, the problem that the reagent use time is wasted due to early reconstitution of the second basic reagent is avoided.
Alternatively, the information of the item to be measured of the sample analyzer 100 may be obtained by the sample injection device 10 or may be input by an operator manipulating an input device.
Alternatively, the information acquiring mechanism 22 may acquire the reagent information corresponding to the reagent stored in the second reagent container, in such a manner that the reagent supplying mechanism 21 rotates the second reagent sites 2112 one by one to the reagent information acquiring site, so that the information acquiring mechanism 22 acquires the reagent information corresponding to the reagent stored in the second reagent container in each of the second reagent sites 2112 at the reagent information acquiring site.
Optionally, the information obtaining mechanism 22 may be movably configured, and the manner in which the information obtaining mechanism 22 obtains the reagent information corresponding to the reagent stored in the second reagent container may be that the controller 50 controls the information obtaining mechanism 22 to move to the second reagent position 2112 where the information is to be collected, and collect the reagent information corresponding to the reagent stored in the second reagent container in the current second reagent position 2112 until the collection of the reagent information corresponding to the reagent stored in the second reagent container in all the second reagent positions 2112 is completed.
In some embodiments, the reagent dispensing mechanism performs the first reagent dispensing operation and the target reagent dispensing operation in different reagent dispensing cycles.
Illustratively, the reagent dispensing mechanism performs a first reagent dispensing operation during a kth reagent dispensing cycle and performs a target reagent dispensing operation during a kth+n reagent dispensing cycle, N being greater than or equal to 1. The reagent dispensing mechanism 24 performs corresponding reagent dispensing operations in different reagent dispensing cycles, thereby achieving full utilization of the overall machine timing.
In some embodiments, the controller 50, in controlling the reagent dispensing mechanism 24 to perform a first reagent dispensing operation, performs:
Controlling the reagent dispensing mechanism 24 to perform a first mixing operation comprising aspirating the first base reagent from the first reagent container and discharging the first base reagent to the second reagent container to mix the first base reagent and the second base reagent to form the first reagent; after the first mixing operation is completed, the reagent dispensing mechanism 24 is controlled to perform a first suction and discharge mixing operation on the first reagent in the second reagent container.
Illustratively, the reagent dispensing mechanism 23 drives the reagent supply mechanism 21 to move the first reagent container disposed on the first reagent station 2111 to the reagent dispensing position, such that the reagent dispensing mechanism 24 draws the first base reagent toward the first reagent container at the reagent dispensing position.
After the first basic reagent is sucked, the reagent dispensing mechanism 23 drives the reagent supplying mechanism 21 to move the second reagent container having the second basic reagent placed on the second reagent position 2112 to the reagent dispensing position, so that the reagent dispensing mechanism 24 dispenses the first basic reagent into the second basic reagent in the second reagent container at the reagent dispensing position, and mixed dilution of the second basic reagent and the first basic reagent is achieved to obtain the first reagent.
After the first reagent is configured, the reagent dispensing mechanism 24 is used for uniformly mixing the reconstituted first reagent, so that the mixing effect of the second basic reagent and the first basic reagent is better, and the multiplexing reagent dispensing mechanism 24 is used for uniformly mixing, so that a uniform mixing mechanism for uniformly mixing the target reagent is not required to be independently arranged, the miniaturization of equipment is facilitated, and the manufacturing cost of the equipment is saved.
In some embodiments, the controller 50 performs, in controlling the reagent dispensing mechanism 21 to aspirate a first base reagent from a first reagent container and discharge the first base reagent to the second reagent container:
the reagent dispensing mechanism 21 is controlled to aspirate a first base reagent from a first reagent container and to discharge the first base reagent into a container wall of a second reagent container carrying a second base reagent so that the first base reagent flows along the container wall into the second reagent container.
Referring to fig. 5, for example, if there are more bubbles in the first reagent, the accuracy of reagent sucking may be affected, so that the reliability and accuracy of the later detection data may be affected, so, in order to reduce the amount of bubbles generated in the process of disposing the first reagent, the reagent dispensing mechanism 21 drives the reagent needle 241 to move down to a predetermined distance H from the bottom of the second reagent container after sucking the first base reagent from the first reagent container, and drains the container wall of the second reagent container, so that the discharged first base reagent flows along the tube wall to the second base reagent, and further reduces the amount of bubbles generated by the mutual impact of the first base reagent and the second base reagent.
In some embodiments, the reagent dispensing mechanism 24 includes a reagent needle 241 and a power assembly 240 coupled to the reagent needle 241 for powering the reagent needle 241 for performing a reagent dispensing operation;
the controller 50 performs the first sucking, spitting and mixing operation on the first reagent in the second reagent container by controlling the reagent dispensing mechanism 24:
controlling the power assembly 240 to drive the needlepoint of the reagent needle 241 to descend to a preset position below the liquid surface of the first reagent in the second reagent container, and controlling the power assembly 240 to drive the reagent needle 241 to perform at least one first sucking and spitting operation, wherein the reagent needle 241 sucks a first amount of the first reagent from the second reagent container and discharges a second amount of the first reagent to the second reagent container in each first sucking and spitting operation.
Alternatively, the reagent dispensing mechanism 24 always has the tip of the reagent needle 241 below the liquid surface of the first reagent in the second reagent container during the execution of the pipetting mixing operation. Alternatively, the reagent dispensing mechanism 24 may be in the process of performing the pipetting and mixing operation in which the tip of the reagent needle 241 is lowered to the first position below the liquid surface of the first reagent in the second reagent container, or in the process of bringing the tip of the reagent needle 241 into contact with the liquid surface of the first reagent and lowering to the first position below the liquid surface of the first reagent in the second reagent container.
Referring to fig. 6, in an exemplary process of mixing the first reagent, the power assembly 240 drives the reagent needle 241 to a first position below the liquid level of the first reagent in the second reagent container, at this time, a space H1 exists between the tip of the reagent needle 241 and the bottom of the second reagent container, the liquid level of the first reagent in the second reagent container is H2, and the change of the liquid level caused by the entering of the reagent needle below the liquid level is less affected by the entering of the reagent needle below the liquid level, so that the change of the liquid level caused by the entering of the reagent needle below the liquid level of the target reagent is ignored.
After the reagent needle 241 is below the level of the first reagent, the power assembly 240 drives the reagent needle 241 to draw a first amount of the first reagent, at which point the level of the second reagent container is H3.
After the first amount of the first reagent is sucked, the second amount of the first reagent is discharged to the second reagent container, and at this time, the liquid level of the second reagent container is H4, and the discharged first reagent impacts the first reagent carried in the second reagent container, so that the first reagent is uniformly mixed.
Optionally, the first amount is greater than or equal to the second amount, i.e. H2 > H3 > H4, e.g. the second amount is 0.4 to 0.9 times the first amount.
Optionally, the controller 50 controls the power assembly 240 to drive the reagent needle 241 to perform the first suction and spit mixing operation at least 2 times below the liquid surface of the first reagent, and the first reagent amount discharged by the last suction and spit mixing operation of the reagent needle 241 is substantially equal to the first reagent amount sucked.
For example, the reagent needle 241 needs to perform M+1 mixing operations on the first reagent, M is greater than or equal to 2, and in the M+1th mixing operation after performing M times of sucking and spitting mixing operations, after the reagent needle 241 is below the liquid surface of the first reagent, the power assembly 240 drives the reagent needle 241 to suck the first reagent, and at this time, the liquid surface height of the second reagent container is H3. After the first reagent is sucked up, the power unit 240 drives the reagent needle 241 to discharge the first reagent to the second reagent container, and at this time, the liquid level of the second reagent container is H4.
In some embodiments, the first mixing operation and the first pipetting mixing operation are performed in different ones of the reagent dispensing cycles.
Illustratively, the reagent dispensing cycle includes a first action sub-cycle in which the reagent dispensing mechanism 24 is controlled to aspirate a first base reagent from the first reagent container and expel the first base reagent to the second reagent container carrying the second base reagent;
in the first action sub-period in the k+n-th reagent dispensing period, the reagent dispensing mechanism 24 is controlled to perform a mixing operation on the first reagent in the second reagent container, wherein N is not less than 1, preferably N is not less than 3. Alternatively, K.gtoreq.1, preferably K.gtoreq.4.
Illustratively, the reagent dispensing mechanism 24 performs the reagent dispensing operation and the mixing operation separately in different reagent dispensing cycles, and the reagent dispensing mechanism 24 performs the mixing operation in a corresponding reagent dispensing cycle after the reagent dispensing operation is completed. By performing the configuration operation of the target reagent and the blending operation of the target reagent in different reagent dispensing cycles, it is unnecessary to increase the blending time sequence by changing the time sequence of the whole machine during the blending operation of the reagent dispensing mechanism 24, thereby ensuring the sample analysis performance of the sample analyzer without affecting the time sequence operation of the whole machine and the subsystem of the sample analyzer.
In some embodiments, the controller 50 is further configured to, prior to the tip of the reagent needle 241 being lowered into contact with the liquid surface of the first reagent in the second reagent container:
The power assembly 240 is controlled to drive the reagent needle 241 to perform a protective medium sucking operation to suck a third amount of protective medium. Optionally, the protective medium is air, and the third amount is greater than the first amount.
Illustratively, the target agent is isolated by inhalation of a protective medium to effectively protect the target agent from environmental factors.
In some embodiments, the controller 50, in controlling the reagent dispensing mechanism 24 to perform a target reagent configuration operation to configure a target reagent with the first reagent and the third base reagent, performs:
Controlling the reagent dispensing mechanism 24 to perform a second mixing operation including aspirating the third base reagent and dispensing the third base reagent to the first reagent to mix the first reagent and the third base reagent to form a target reagent;
after the second mixing operation is completed, the reagent dispensing mechanism 24 is controlled to perform a second suction and discharge mixing operation on the first target reagent so as to mix the target reagent.
Illustratively, in configuring the target reagent, the reagent dispensing mechanism 24 first quantitatively draws the third base reagent into the reagent container carrying the third base reagent and fills the drawn third base reagent into the reagent container carrying the first reagent to configure the third base reagent and the first reagent to form the target reagent.
After the target reagent is placed, the reagent dispensing mechanism 24 is inserted into the liquid surface of the target reagent, and suctions, spits and mixes the target reagent.
In some embodiments, the controller 50 performs, during the control of the reagent dispensing mechanism 24 to perform the second pipetting operation on the first target reagent:
Controlling the power assembly 240 to drive the needle tip of the reagent needle 241 to descend to a second position below the liquid surface of the reagent container carrying the target reagent, and controlling the power assembly 240 to drive the reagent needle 241 to perform at least one first pipetting mixing operation, wherein the reagent needle 241 aspirates a third amount of the target reagent from the reagent container carrying the target reagent and discharges a fourth amount of the target reagent, optionally, the third amount is greater than or equal to the fourth amount, each time the first pipetting mixing operation.
Alternatively, the reagent dispensing mechanism 24 is always under the liquid surface of the target reagent in the reagent container carrying the target reagent during the execution of the second suction and discharge mixing operation. Alternatively, the reagent dispensing mechanism 24 may be, without limitation, a process in which the tip of the reagent needle 241 is lowered to a second position below the liquid surface of the target reagent in the reagent container carrying the target reagent after performing the suction and discharge mixing operation, or the tip of the reagent needle 241 is brought into contact with the liquid surface of the target reagent and lowered to a second position below the liquid surface of the target reagent in the reagent container carrying the target reagent.
In some embodiments, the reagent placement sites further comprise fourth reagent placement sites for placement of fourth base reagents, the fourth reagent containers being used to place fourth base reagents, the controller 50 performing, in controlling the reagent dispensing mechanism 24 to perform a target reagent configuration operation to configure a target reagent with the first reagent and the third base reagents:
controlling the reagent dispensing mechanism 24 to perform a second reagent dispensing operation comprising aspirating the fourth base reagent from the fourth reagent container and discharging the fourth base reagent to the third reagent container to mix the third base reagent and the fourth base reagent to form a second reagent;
After the second reagent preparing operation is completed, controlling the reagent dispensing mechanism 24 to perform a third mixing operation including sucking the second reagent and filling the second reagent into the first reagent so that the first reagent and the second reagent are mixed to form a target reagent;
after the third mixing operation is completed, the reagent dispensing mechanism 24 is controlled to perform the suction and discharge mixing operation on the target reagent so as to mix the target reagent uniformly.
The fourth base reagent is illustratively a diluent, and in the process of preparing the target reagent, the third base reagent may be diluted to obtain the second reagent, and the second reagent and the first reagent may be mixed to obtain the target reagent. After the target reagent is obtained, the reagent needle 241 of the reagent dispensing mechanism 24 is inserted below the liquid surface of the target reagent, and performs the sucking and spitting operation, and the specific sucking and spitting operation may refer to the sucking and spitting operation of the first reagent, which will not be described herein.
In some embodiments, the reagent placement sites further comprise fourth reagent placement sites 2114 for placement of fourth reagent containers for placement of fourth base reagents, the controller 50 further being for:
controlling the reagent dispensing mechanism 24 to perform a second reagent dispensing operation comprising aspirating the fourth base reagent from the fourth reagent container and discharging the fourth base reagent to the third reagent container to mix the third base reagent and the fourth base reagent to form a second reagent;
After the second reagent preparing operation is completed, the reagent dispensing mechanism 24 is controlled to perform the sucking and discharging operation on the second reagent so as to mix the second reagent uniformly.
Optionally, the third basic reagent is a first IC reagent, the fourth basic reagent is a diluent, and the second IC reagent is obtained after mixing the fourth basic reagent and the third basic reagent, that is, the diluent is used in the mixing process of the third basic reagent, the first IC reagent is diluted to obtain the second IC reagent, that is, both the first IC reagent and the second IC reagent may be referred to as IC reagents, but the reagent concentration of the second IC reagent is smaller than that of the first IC reagent. The IC reagent formed using the third base reagent and the fourth base reagent configuration may be applied in a nucleic acid sample extraction reaction process.
In some embodiments, the reagent processing apparatus 20 further comprises a blending mechanism, and the controller 50 is further configured to:
After the first reagent is configured, controlling the mixing mechanism to perform mixing operation on the first reagent;
and after the configuration of the first target reagent is finished, controlling the mixing mechanism to perform mixing operation on the target reagent.
The reagent processing apparatus 20 is provided with a mixing mechanism for mixing the prepared corresponding reagents, wherein the mixing mechanism includes, but is not limited to, a vibration mixing mechanism for performing a vibration mixing operation on the reagents, and an ultrasonic mixing mechanism for performing an ultrasonic mixing operation on the reagents.
In some embodiments, the controller 50 is further configured to:
Controlling the information acquisition mechanism 22 to acquire reagent information corresponding to the reagent stored in the reagent container in the reagent storage position 211, and determining a reagent configuration strategy according to the information of the item to be detected of the sample analyzer and the reagent information;
Controlling the reagent dispensing mechanism to perform a reagent dispensing operation according to the reagent configuration strategy so as to dispense a base reagent for configuring a reagent to be configured;
during the reagent dispensing cycle, the reagent dispensing mechanism 24 is controlled to perform a reagent dispensing operation in accordance with the reagent dispensing strategy to dispense the reagent to be dispensed with the dispensed base reagent.
Illustratively, the reagent to be dispensed includes at least one of a first reagent, a second reagent, and a target reagent, e.g., when it is determined that the reagent to be dispensed is the first reagent according to the project information to be tested, the reagent dispensing mechanism 24 is controlled to dispense the first and second base reagents required for the first reagent according to the reagent dispensing strategy.
The reagent preparing method according to the embodiment of the present application will be described below with reference to the working principle of the sample analyzer 100.
Referring to fig. 7, the embodiment of the application further provides a reagent configuration method applied to the sample analyzer 100, and the reagent configuration method includes steps S101 to S102.
Step S101: controlling a reagent dispensing mechanism of the sample analyzer to perform a first reagent configuration operation for configuring the first reagent during a reagent dispensing cycle of the sample analyzer;
step S102: after the first reagent is configured, controlling the reagent dispensing mechanism to execute a target reagent configuration operation so as to form a target reagent by using the first reagent and the third basic reagent, wherein the first reagent is a PCR reagent, and the third basic reagent is an IC reagent.
In some embodiments, the first reagent dispensing operation and the target reagent dispensing operation are performed in different ones of the reagent dispensing cycles.
In some embodiments, the controlling the reagent dispensing mechanism to perform a first reagent configuration operation comprises:
Controlling the reagent dispensing mechanism to perform a first mixing operation comprising aspirating the first base reagent from the first reagent container and discharging the first base reagent to the second reagent container to mix the first base reagent and the second base reagent to form the first reagent;
and after the first mixing operation is finished, controlling the reagent dispensing mechanism to execute a first sucking and spitting uniform mixing operation on the first reagent in the second reagent container.
In some embodiments, the reagent dispensing mechanism includes a reagent needle and a power assembly connected to the reagent needle and configured to power the reagent needle to perform a reagent dispensing operation, the controlling the reagent dispensing mechanism to perform a first pipetting mixing operation on a first reagent in the second reagent container includes:
And controlling the power assembly to drive the needle tip of the reagent needle to descend to a preset position below the liquid level of the first reagent in the second reagent container, and controlling the power assembly to drive the reagent needle to perform at least one first sucking and spitting mixing operation, wherein in each first sucking and spitting mixing operation, the reagent needle sucks a first amount of the first reagent from the second reagent container and discharges a second amount of the first reagent to the second reagent container, and the first amount is larger than the second amount.
In some embodiments, before the tip of the reagent needle descends into contact with the liquid surface of the first reagent in the second reagent container, the method further comprises:
Controlling the power assembly to drive the reagent needle to perform a protective medium sucking operation to suck a third amount of protective medium.
In some embodiments, the first mixing operation and the first pipetting mixing operation are performed in different ones of the reagent dispensing cycles.
In some embodiments, the controlling the reagent dispensing mechanism to perform a target reagent configuration operation to form a target reagent using the first reagent and the third base reagent configuration comprises:
In some embodiments, the controlling the reagent dispensing mechanism to perform a target reagent configuration operation to configure a target reagent with the first reagent and the third base reagent comprises:
Controlling the reagent dispensing mechanism to perform a second mixing operation, the second mixing operation comprising aspirating the third base reagent and dispensing the third base reagent to the first reagent to mix the first reagent and the third base reagent to form a target reagent;
And after the second mixing operation is finished, controlling the reagent dispensing mechanism to execute a second sucking and spitting uniform mixing operation on the first target reagent.
In some embodiments, the reagent placement site further comprises a fourth reagent placement site for placement of a fourth reagent container for placement of a fourth base reagent, the controlling the reagent dispensing mechanism to perform a target reagent configuration operation to configure a target reagent with the first reagent and the third base reagent, comprising:
Controlling the reagent dispensing mechanism to perform a second reagent dispensing operation comprising aspirating the fourth base reagent from the fourth reagent container and discharging the fourth base reagent to the third reagent container to mix the third base reagent and the fourth base reagent to form a second reagent;
After the second reagent configuration operation is completed, controlling the reagent dispensing mechanism to execute a third mixing operation, wherein the third mixing operation comprises sucking the second reagent and filling the second reagent into the first reagent so as to mix the first reagent and the second reagent to form a target reagent;
And after the third mixing operation is finished, controlling the reagent dispensing mechanism to execute a third sucking and spitting mixing operation on the target reagent. In some embodiments, the sample analyzer further comprises an oscillating mixing mechanism, the method further comprising:
After the first reagent is configured, controlling the mixing mechanism to perform oscillation mixing operation on the first reagent;
after the second reagent is configured, controlling the mixing mechanism to perform oscillation mixing operation on the second reagent;
and after the configuration of the first target reagent is finished, controlling the mixing mechanism to perform oscillation mixing operation on the target reagent.
It should be noted that, for convenience and brevity of description, specific working procedures of the reagent configuration method described above may refer to corresponding working procedures of the sample analyzer, and will not be described herein.
It is to be understood that the terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
It should also be understood that the term "and/or" as used in the present specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The foregoing embodiment numbers of the present application are merely for the purpose of description, and do not represent the advantages or disadvantages of the embodiments. While the application has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions of equivalents may be made and equivalents will be apparent to those skilled in the art without departing from the scope of the application. Therefore, the protection scope of the application is subject to the protection scope of the claims.

Claims (11)

1.一种样本分析仪，其特征在于，包括：1. A sample analyzer, comprising: 试剂供应机构，设置有用于放置试剂容器的试剂放置位，所述试剂放置位包括用于放置第一试剂容器的第一试剂放置位、用于放置第二试剂容器的第二试剂放置位、用于放置第三试剂容器的第三试剂放置位，其中，所述第一试剂容器存放用于配置第一试剂的第一基础试剂，所述第二试剂容器存放用于配置第一试剂的第二基础试剂，所述第三试剂容器存放第三基础试剂；The reagent supply mechanism is provided with reagent placement positions for placing reagent containers, wherein the reagent placement positions include a first reagent placement position for placing a first reagent container, a second reagent placement position for placing a second reagent container, and a third reagent placement position for placing a third reagent container, wherein the first reagent container stores a first basic reagent for configuring the first reagent, the second reagent container stores a second basic reagent for configuring the first reagent, and the third reagent container stores a third basic reagent; 试剂分注机构，至少用于在试剂分注周期内执行试剂分注操作，以将试剂分注至对应的试剂容器；及A reagent dispensing mechanism, at least used to perform a reagent dispensing operation within a reagent dispensing cycle to dispense the reagent into a corresponding reagent container; and 控制器，至少用于：A controller, at least for: 在所述试剂分注周期内，控制所述试剂分注机构执行第一试剂配置操作，所述第一试剂配置操作用于配置所述第一试剂；During the reagent dispensing cycle, controlling the reagent dispensing mechanism to perform a first reagent configuration operation, wherein the first reagent configuration operation is used to configure the first reagent; 在所述第一试剂配置操作执行完成后，控制所述试剂分注机构执行目标试剂配置操作，以利用所述第一试剂和所述第三基础试剂配置目标试剂，所述第一试剂为PCR试剂，所述第三基础试剂为IC试剂。After the first reagent configuration operation is completed, the reagent dispensing mechanism is controlled to perform a target reagent configuration operation to configure a target reagent using the first reagent and the third basic reagent, wherein the first reagent is a PCR reagent and the third basic reagent is an IC reagent. 2.根据权利要求1所述的样本分析仪，其特征在于，所述第一试剂配置操作及所述目标试剂配置操作在不同的所述试剂分注周期中执行。2 . The sample analyzer according to claim 1 , wherein the first reagent configuration operation and the target reagent configuration operation are performed in different reagent dispensing cycles. 3 . 3.根据权利要求1所述的样本分析仪，其特征在于，所述控制器在控制所述试剂分注机构执行第一试剂配置操作过程中，执行：3. The sample analyzer according to claim 1, wherein the controller, in controlling the reagent dispensing mechanism to perform the first reagent configuration operation, executes: 控制所述试剂分注机构执行第一混合操作，所述第一混合操作包括从所述第一试剂容器吸取所述第一基础试剂，并将所述第一基础试剂排放至所述第二试剂容器，以使所述第一基础试剂和所述第二基础试剂混合形成所述第一试剂；Controlling the reagent dispensing mechanism to perform a first mixing operation, wherein the first mixing operation includes sucking the first basic reagent from the first reagent container and discharging the first basic reagent into the second reagent container, so that the first basic reagent and the second basic reagent are mixed to form the first reagent; 在所述第一混合操作执行完成后，控制所述试剂分注机构对所述第二试剂容器内的第一试剂执行第一吸吐混匀操作。After the first mixing operation is completed, the reagent dispensing mechanism is controlled to perform a first suction and discharge mixing operation on the first reagent in the second reagent container. 4.根据权利要求3所述的样本分析仪，其特征在于，所述试剂分注机构包括试剂针及与所述试剂针连接，并用于为试剂针执行试剂分注操作提供动力的动力组件，所述控制器在控制所述试剂分注机构对所述第二试剂容器内的第一试剂执行第一吸吐混匀操作过程中，执行：4. The sample analyzer according to claim 3, characterized in that the reagent dispensing mechanism comprises a reagent needle and a power component connected to the reagent needle and used to provide power for the reagent needle to perform a reagent dispensing operation, and the controller performs the following when controlling the reagent dispensing mechanism to perform a first suction and dispensing mixing operation on the first reagent in the second reagent container: 控制所述动力组件驱动所述试剂针的针尖下降至所述第二试剂容器内第一试剂的液面之下的预设位置，并控制所述动力组件驱动所述试剂针执行至少一次第一吸吐混匀操作，其中，在每次所述第一吸吐混匀操作中，所述试剂针从所述第二试剂容器中吸取第一量的所述第一试剂，并向所述第二试剂容器排出第二量的所述第一试剂，所述第一量大于所述第二量。The power component is controlled to drive the needle tip of the reagent needle to descend to a preset position below the liquid surface of the first reagent in the second reagent container, and the power component is controlled to drive the reagent needle to perform at least one first suction and exhalation mixing operation, wherein, in each of the first suction and exhalation mixing operations, the reagent needle absorbs a first amount of the first reagent from the second reagent container and discharges a second amount of the first reagent into the second reagent container, and the first amount is greater than the second amount. 5.根据权利要求4所述的样本分析仪，其特征在于，在所述试剂针的针尖下降至与所述第二试剂容器内所述第一试剂的液面接触前，所述控制器还用于：5. The sample analyzer according to claim 4, characterized in that before the tip of the reagent needle descends to contact the liquid surface of the first reagent in the second reagent container, the controller is further configured to: 控制所述动力组件驱动所述试剂针执行保护介质吸取操作，以吸取第三量的保护介质。The power assembly is controlled to drive the reagent needle to perform a protective medium aspirating operation to aspirate a third amount of protective medium. 6.根据权利要求3所述的样本分析仪，其特征在于，所述第一混合操作和所述第一吸吐混匀操作在不同的所述试剂分注周期内执行。6 . The sample analyzer according to claim 3 , wherein the first mixing operation and the first suction and discharge mixing operation are performed in different reagent dispensing cycles. 7.根据权利要求1所述的样本分析仪，其特征在于，所述控制器在控制所述试剂分注机构执行目标试剂配置操作，以利用所述第一试剂和所述第三基础试剂配置目标试剂过程中，执行：7. The sample analyzer according to claim 1, wherein the controller, in controlling the reagent dispensing mechanism to perform a target reagent configuration operation so as to configure the target reagent using the first reagent and the third basic reagent, executes: 控制所述试剂分注机构执行第二混合操作，所述第二混合操作包括吸取所述第三基础试剂，并将所述第三基础试剂加注至所述第一试剂，以使所述第一试剂和所述第三基础试剂混合形成目标试剂；Controlling the reagent dispensing mechanism to perform a second mixing operation, wherein the second mixing operation includes sucking the third basic reagent and injecting the third basic reagent into the first reagent, so that the first reagent and the third basic reagent are mixed to form a target reagent; 在所述第二混合操作执行完成后，控制所述试剂分注机构对所述第目标试剂执行第二吸吐混匀操作。After the second mixing operation is completed, the reagent dispensing mechanism is controlled to perform a second suction and discharge mixing operation on the first target reagent. 8.根据权利要求1所述的样本分析仪，其特征在于，所述试剂放置位还包括用于放置第四试剂容器的第四试剂放置位，所述第四试剂容器用于放置第四基础试剂，所述控制器还用于：8. The sample analyzer according to claim 1, characterized in that the reagent placement position further comprises a fourth reagent placement position for placing a fourth reagent container, the fourth reagent container is used to place a fourth basic reagent, and the controller is further used to: 控制所述试剂分注机构执行第二试剂配置操作，所述第二试剂配置操作包括从所述第四试剂容器吸取所述第四基础试剂，并将所述第四基础试剂排放至所述第三试剂容器，以使所述第三基础试剂和所述第四基础试剂混合形成第二试剂；controlling the reagent dispensing mechanism to perform a second reagent dispensing operation, wherein the second reagent dispensing operation includes sucking the fourth basic reagent from the fourth reagent container and discharging the fourth basic reagent into the third reagent container so that the third basic reagent and the fourth basic reagent are mixed to form a second reagent; 在所述第二试剂配置操作执行完成后，控制所述试剂分注机构对所述第二试剂执行第三吸吐混匀操作。After the second reagent configuration operation is completed, the reagent dispensing mechanism is controlled to perform a third suction and discharge mixing operation on the second reagent. 9.根据权利要求1所述的样本分析仪，其特征在于，所述样本分析仪还包括振荡混匀机构，所述控制器还用于：9. The sample analyzer according to claim 1, characterized in that the sample analyzer further comprises an oscillating mixing mechanism, and the controller is further used for: 在所述第一试剂配置完成后，控制所述混匀机构对所述第一试剂执行振荡混匀操作；After the first reagent is configured, controlling the mixing mechanism to perform an oscillation mixing operation on the first reagent; 在所述第目标试剂配置完成后，控制所述混匀机构对所述目标试剂执行振荡混匀操作。After the first target reagent is configured, the mixing mechanism is controlled to perform an oscillation mixing operation on the target reagent. 10.一种试剂配置方法，应用于样本分析仪，其特征在于，所述方法包括：10. A reagent configuration method, applied to a sample analyzer, characterized in that the method comprises: 在所述样本分析仪的试剂分注周期内，控制所述样本分析仪的试剂分注机构执行第一试剂配置操作，所述第一试剂配置操作用于配置所述第一试剂；During a reagent dispensing cycle of the sample analyzer, controlling a reagent dispensing mechanism of the sample analyzer to perform a first reagent configuration operation, wherein the first reagent configuration operation is used to configure the first reagent; 在所述第一试剂配置操作执行完成后，控制所述试剂分注机构执行目标试剂配置操作，以利用所述第一试剂和所述第三基础试剂配置目标试剂，所述第一试剂为PCR试剂，所述第三基础试剂为IC试剂。After the first reagent configuration operation is completed, the reagent dispensing mechanism is controlled to perform a target reagent configuration operation to configure a target reagent using the first reagent and the third basic reagent, wherein the first reagent is a PCR reagent and the third basic reagent is an IC reagent. 11.一种样本分析仪，其特征在于，包括：11. A sample analyzer, comprising: 试剂供应机构，设置有用于放置试剂容器的多个试剂放置位，所述试剂放置位用于放置试剂容器，所述试剂容器用于存放基础试剂；The reagent supply mechanism is provided with a plurality of reagent placement positions for placing reagent containers, wherein the reagent placement positions are used to place reagent containers, and the reagent containers are used to store basic reagents; 信息获取机构，用于获取所述试剂放置位中试剂容器所存放试剂对应的试剂信息；An information acquisition mechanism, used for acquiring reagent information corresponding to the reagent stored in the reagent container in the reagent placement position; 试剂分配机构，用于执行试剂分配操作；A reagent dispensing mechanism, used for performing a reagent dispensing operation; 试剂分注机构，至少用于在试剂分注周期内执行试剂分注操作，以将试剂分注至对应的试剂容器；及A reagent dispensing mechanism, at least used to perform a reagent dispensing operation within a reagent dispensing cycle to dispense the reagent into a corresponding reagent container; and 控制器，至少用于：A controller, at least for: 控制所述信息获取机构获取所述试剂放置位中试剂容器所存放试剂对应的试剂信息，及根据所述样本分析仪的待测项目信息及所述试剂信息确定试剂配置策略；Controlling the information acquisition mechanism to acquire reagent information corresponding to the reagent stored in the reagent container in the reagent placement position, and determining a reagent configuration strategy according to the test item information of the sample analyzer and the reagent information; 根据所述试剂配置策略，控制所述试剂分配机构执行试剂分配操作，以分配用于配置待配置试剂的基础试剂；According to the reagent configuration strategy, controlling the reagent dispensing mechanism to perform a reagent dispensing operation to dispense a basic reagent for configuring the reagent to be configured; 在所述试剂分注周期内，根据所述试剂配置策略控制所述试剂分注机构执行试剂配置操作，以利用所分配的基础试剂配置所述待配置试剂。During the reagent dispensing cycle, the reagent dispensing mechanism is controlled to perform a reagent configuration operation according to the reagent configuration strategy, so as to configure the reagent to be configured using the allocated basic reagent.
CN202211675850.2A 2022-12-26 2022-12-26 Sample analyzer and reagent configuration method Pending CN118258676A (en)

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