CN118319829B - A skin care composition containing lactobacillus/algae extract fermentation product and its preparation method and application - Google Patents
A skin care composition containing lactobacillus/algae extract fermentation product and its preparation method and application Download PDFInfo
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- CN118319829B CN118319829B CN202410467320.1A CN202410467320A CN118319829B CN 118319829 B CN118319829 B CN 118319829B CN 202410467320 A CN202410467320 A CN 202410467320A CN 118319829 B CN118319829 B CN 118319829B
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Classifications
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Landscapes
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- Cosmetics (AREA)
Abstract
本发明涉及一种含乳酸杆菌/藻提取物发酵产物的护肤组合物及其制备方法和应用，所述含乳酸杆菌/藻提取物发酵产物的护肤组合物包括乳酸杆菌/藻提取物发酵产物、球藻组合物、植物提取物、油脂、乳化剂和保湿剂。本发明创造性地发现乳酸杆菌/藻提取物发酵产物、球藻组合物、植物提取物、油脂四种组分搭配使用对肌肤具有显著的抗氧化、保湿、抗炎及维持皮肤表面微生物平衡的效果，且四者在上述功效上具有一定的协同增效效果，非常适合用于护肤类产品，温和不刺激，且稳定性好。The present invention relates to a skin care composition containing a lactobacillus/algae extract fermentation product, a preparation method and an application thereof. The skin care composition containing a lactobacillus/algae extract fermentation product comprises a lactobacillus/algae extract fermentation product, a spherical algae composition, a plant extract, oil, an emulsifier and a moisturizer. The present invention creatively finds that the combination of the four components of the lactobacillus/algae extract fermentation product, the spherical algae composition, the plant extract and the oil has significant antioxidant, moisturizing, anti-inflammatory and skin surface microbial balance effects on the skin, and the four components have a certain synergistic effect on the above effects, and are very suitable for use in skin care products, are mild and non-irritating, and have good stability.
Description
Technical Field
The invention belongs to the technical field of daily cosmetics, and particularly relates to a skin care composition containing lactobacillus/algae extract fermentation products, and a preparation method and application thereof.
Background
The skin is continuously contacted with the outside, such as external pollution, ultraviolet irradiation and other factors, so that free radicals are continuously generated, the capability of removing the free radicals of a human body is reduced due to the increase of the age and certain in vitro factors, and excessive free radicals have destructive effects on unsaturated fatty acids, protein molecules, nucleic acid molecules, extracellular soluble components, membrane lipids and the like, so that wrinkles, sagging and subcutaneous fat are reduced, the skin is moisturized and the function of maintaining elasticity is lost, skin tissues are damaged, aging is caused, and the problems of reduced metabolism of epidermis, disturbed skin flora, damaged epidermis repair capability, reduced barrier protection capability and the like are also caused.
CN116509736a discloses a skin barrier repair and cell repair anti-wrinkle composition, which comprises, by weight, 50-70 parts of component a, 10-20 parts of component B, and 10-20 parts of component C. The invention constructs the liquid crystal structure and the bionic cortex through mixing various components, can effectively penetrate through the stratum corneum, has high bioavailability, forms liposome wrapping components, has excellent stability, can simultaneously achieve barrier repair and cell repair of the epidermis layer and the dermis layer, can effectively repair damaged cells, repair skin barriers, can accelerate metabolism, accelerate differentiation of cells, stimulate generation of ceramide and other components, and promotes self barrier repair and cell repair of cells. The components used in the composition of the invention are safe and nonirritating, and can be applied to various skins.
CN109846740A discloses a skin care composition, a preparation method and application thereof, wherein the composition comprises, based on 100% by weight of the composition, 0.001-20% (w/w) of pyruvic acid and salts thereof, 0.001-15% (w/w) of ketovaleric acid and derivatives thereof, and 65-90% (w/w) of humectant. The composition has greatly improved oxidation resistance, has the capability of repairing injury, and can effectively improve the skin water deficiency state.
However, although the above-mentioned products have a certain skin care effect, because of the self-barrier function of human skin, the active ingredients are difficult to enter into the deep layer of skin to exert the effect, and therefore, it is necessary to develop a skin care product having better and safer effects such as oxidation resistance and skin repair.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a skin care composition containing lactobacillus/algae extract fermentation product, and a preparation method and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
In a first aspect, the present invention provides a skin care composition comprising a lactobacillus/algae extract fermentation product, the skin care composition comprising a lactobacillus/algae extract fermentation product, a chlorella composition, a plant extract, a lipid, an emulsifier, and a humectant.
The lactobacillus/algae extract fermentation product contains alpha-hydroxy acid (AHA) such as lactic acid and other bioactive substances, can help balance the microbiota of skin, accelerate the generation of new cells, strengthen the natural barrier of skin, protect the skin from free radicals, improve the resistance of the skin to external stimulus and pollution, have excellent effects of resisting oxidation, moisturizing, resisting inflammation and maintaining the balance of skin microorganisms, can help the skin to keep moisture, improve the moisturizing capability of the skin, make the skin look more moist and smooth, and can improve the permeability and effect of other active ingredients in a formula, and the plant extract can reduce skin inflammation and has potential benefits for improving sensitive skin or inflammatory skin diseases (such as acne).
The invention creatively discovers that the lactobacillus/algae extract fermentation product, the chlorella composition, the plant extract and the grease have remarkable effects of resisting oxidation, moisturizing, resisting inflammation and maintaining the balance of microorganisms on the surface of skin when being used together, and the four have a certain synergistic effect on the effects, so that the invention is very suitable for skin care products, is mild and does not irritate, and has good stability.
Preferably, the skin care composition containing lactobacillus/algae extract fermentation product comprises 0.5-20 parts of lactobacillus/algae extract fermentation product, 1-10 parts of chlorella composition, 1-15 parts of plant extract, 1-10 parts of grease, 1-10 parts of emulsifier and 1-10 parts of humectant.
The lactobacillus/algae extract fermentation product may be added in an amount of 0.8 parts, 1 part, 2 parts, 5 parts, 7 parts, 9 parts, 10 parts, 13 parts, 15 parts, 17 parts, 19 parts, or the like in the skin care composition containing the lactobacillus/algae extract fermentation product of the present invention;
The adding amount of the chlorella composition can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;
the plant composition may be added in an amount of 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, or the like;
The addition amount of the emulsifier can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;
The humectant may be added in an amount of 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or the like.
Preferably, the skin care composition containing lactobacillus/algae extract fermentation product comprises 3-15 parts of lactobacillus/algae extract fermentation product, 2-9 parts of chlorella composition, 2-12 parts of plant extract, 2-10 parts of grease, 3-9 parts of emulsifier and 2-9 parts of humectant.
Preferably, the skin care composition containing lactobacillus/algae extract fermentation product comprises 5-12 parts of lactobacillus/algae extract fermentation product, 3-8 parts of chlorella composition, 3-10 parts of plant extract, 3-9 parts of grease, 3-7 parts of emulsifier and 2-8 parts of humectant.
Preferably, the chlorella composition comprises an extract of nannochloropsis oculata and an extract of nannochloropsis oculata.
Preferably, the mass ratio of the nannochloropsis oculata extract to the polar nannochloropsis extract is 1 (0.5-10), for example, 1:0.8, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8 or 1:9, etc.
Compared with other chlorella extracts, the chlorella oculopsis extract and the very small chlorella oculopsis extract are matched with other components in the composition, so that the composition can be quickly absorbed by skin and forms a layer of protective film on the surface layer of the skin, thereby reducing water evaporation and playing a better role in moisture preservation.
Preferably, the plant extract comprises any one or a combination of at least two of a compact Luo Mushe/stem extract, a coconut fruit extract, a chamomile extract, an aloe vera leaf extract, a tea leaf extract, a calamus root extract, or a myrrh resin extract.
Preferably, the plant extract is a combination of coconut fruit extract, calamus root extract and myrrh resin extract.
As a preferred technical scheme of the invention, the coconut fruit extract, the calamus root extract and the myrrh resin extract are used in a matched mode, can inhibit the synthesis of inflammatory factors and slow down the release of the inflammatory factors, play an anti-inflammatory role, are natural and non-irritating, can promote the growth of dominant bacteria on the surface of the skin, and maintain the skin micro-ecology.
Preferably, the mass ratio of the coconut fruit extract, the calamus root extract and the myrrh resin extract is (3-5): 2-4): 1-5.
Wherein "3-5" may be 3.1, 3.3, 3.5, 3.7, 3.9, 4, 4.1, 4.3, 4.5, 4.7, 4.9, etc.;
"2-4" may be 2.1, 2.3, 2.5, 2.7, 2.9, 3, 3.1, 3.3, 3.5, 3.7, 3.9, etc.;
"1-5" may be 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, etc.
Preferably, the grease comprises any one or a combination of at least two of butter tree fruit grease, macadamia nut seed oil, olive fruit oil or argania spinosa kernel oil.
Preferably, the lipid is a combination of shea butter, macadamia nut seed oil, olive oil and argania spinosa kernel oil.
Preferably, the mass ratio of the butter tree fruit fat to the macadamia nut seed oil to the olive fruit oil to the argania spinosa kernel oil is (1-3): (3-5): (2-4): (1-5).
Wherein "1-3" may be 1.1, 1.3, 1.5, 1.7, 2, 2.1, 2.3, 2.5, 2.7, 2.9, etc.;
"3-5" may be 3.1, 3.3, 3.5, 3.7, 3.9, 4, 4.1, 4.3, 4.5, 4.7, 4.9, etc.;
"2-4" may be 2.1, 2.3, 2.5, 2.7, 2.9, 3, 3.1, 3.3, 3.5, 3.7, 3.9, etc.;
"1-5" may be 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, etc.
Preferably, the humectant comprises any one or a combination of at least two of sodium polyglutamate, ceramide, butanediol, or 1, 2-hexanediol.
Preferably, the humectant comprises a combination of sodium polyglutamate, ceramide, butanediol, and 1, 2-hexanediol.
Preferably, the mass ratio of the sodium hyaluronate to the ceramide to the butanediol to the 1, 2-hexanediol is (0.1-1): 0.1-5): 1-5: (0.2-0.5.
Wherein "0.1-1" may be 0.1, 0.3, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, etc.;
"0.1-5" may be 0.3, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, or 4.8, etc.;
"1-5" may be 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, etc.;
"0.2-0.5" may be 0.22, 0.25, 0.28, 0.3, 0.32, 0.35, 0.38, 0.4, 0.42, or 0.45, etc.
Preferably, the emulsifier comprises PEG-8C 12-20 acid esters and/or potassium cetyl phosphate.
Preferably, the emulsifier is a combination of PEG-8C 12-20 acid ester and potassium cetyl phosphate.
In the invention, the emulsifier uses the combination of the PEG-8C 12-20 acid ester and the potassium cetyl phosphate, so that the skin care composition and other components can be better emulsified, and the stability of the system is more excellent.
Preferably, the mass ratio of the PEG-8 ester of C12-20 acid to the potassium cetyl phosphate is 1 (0.1-1), and can be, for example, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, or 1:0.9, etc.
Preferably, the skin care composition comprising lactobacillus/algae extract fermentation product further comprises a skin conditioning agent and/or a preservative.
Preferably, the skin care composition comprising lactobacillus/algae extract fermentation product further comprises 1-10 parts (for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.) of skin conditioning agent and 0.1-1 part (for example, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.) of preservative in parts by weight.
Preferably, the skin conditioning agent comprises any one or a combination of at least two of isononyl isononanoate, ascorbic acid, lecithin or squalane.
Preferably, the skin conditioning agent comprises a combination of isononyl isononanoate, ascorbic acid, lecithin and squalane.
The skin conditioner creatively adopts the mode of combining isononyl isononanoate, ascorbic acid, lecithin and squalane, so that the composition can exert more excellent antioxidant and anti-aging effects.
Preferably, the mass ratio of isononyl isononanoate, ascorbic acid, lecithin and squalane is (1-5): 0.5-2): 1-2.5.
Wherein "1-5" may be 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, etc.;
"0.5-2" may be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, etc.;
The first "1-2.5" may be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, etc.;
the second "1-2.5" may be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, etc.
Preferably, the preservative comprises p-hydroxyacetophenone.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
In a second aspect, the present invention provides a method for preparing a skin care composition comprising lactobacillus/algae extract fermentation product as described in the first aspect, said method comprising:
(1) Mixing an emulsifier, a humectant, grease and deionized water, and carrying out high-pressure microjet treatment to obtain a vesicle solution;
(2) Mixing the vesicle solution obtained in the step (1) with a chlorella composition, lactobacillus/algae extract fermentation products and plant extracts, and carrying out high-pressure micro-jet treatment to obtain the skin care composition containing the lactobacillus/algae extract fermentation products.
Preferably, the temperature of the mixing in step (1) is 45-55 ℃, e.g. 46 ℃, 48 ℃, 50 ℃, 52 ℃, 54 ℃ or the like.
Preferably, the high pressure micro-jet treatment in step (1) has a pressure of 200-300MPa and a jet velocity of 500-700m/s, for example, the pressure may be 210MPa, 230MPa, 250MPa, 270MPa or 290MPa, etc., and the jet velocity may be 520m/s, 550m/s, 580m/s, 600m/s, 620m/s, 650m/s or 680m/s, etc.
Preferably, the mixing of step (1) further comprises adding a skin conditioning agent and/or preservative.
Preferably, the temperature of the mixing in step (2) is 40-50 ℃, e.g. 41 ℃, 43 ℃, 45 ℃, 47 ℃, 49 ℃ or the like.
Preferably, the pressure of the high-pressure micro-jet treatment in the step (2) is 200-300MPa, the jet velocity is 500-700m/s, for example, the pressure can be 210MPa, 230MPa, 250MPa, 270MPa or 290MPa, etc., and the jet velocity can be 520m/s, 550m/s, 580m/s, 600m/s, 620m/s, 650m/s or 680m/s, etc.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
In a third aspect, the present invention provides the use of a skin care composition comprising a lactobacillus/algae extract fermentation product as described in the first aspect for the preparation of a cosmetic.
Preferably, the cosmetic comprises essence, lotion, emulsion, cream, facial cleanser or facial mask;
Preferably, the skin care composition containing lactobacillus/algae extract fermentation product is 0.1-15% by mass in the cosmetic, for example, may be 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14% etc.
Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Compared with the prior art, the invention has the following beneficial effects:
(1) The invention creatively discovers that the lactobacillus/algae extract fermentation product, the chlorella composition, the plant extract and the grease have remarkable effects of resisting oxidation, moisturizing, resisting inflammation and maintaining the balance of microorganisms on the surface of skin when being used together, and the four have a certain synergistic effect on the effects, so that the invention is very suitable for skin care products, is mild and does not irritate, and has good stability.
(2) The preparation method of the skin care composition containing lactobacillus/algae extract fermentation product provided by the invention has the advantages of simple operation steps, low cost and easiness in industrial mass production.
Detailed Description
In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The following examples and comparative examples relate to the following partial materials and brand information:
The remaining materials can be used as long as they are purchased from a regular dealer.
Examples 1 to 3
The formulation and the dosage of the hair mask with repairing and softening effects are shown in the following table, and the dosage units of all components in the table are parts;
The preparation method of the skin care composition containing the chlorella vulgaris comprises the following steps:
(1) Mixing an emulsifier, a humectant, grease, a skin conditioning agent, a preservative and deionized water at 50 ℃, and uniformly carrying out high-pressure micro-jet treatment to obtain a vesicle solution, wherein the pressure of the high-pressure micro-jet treatment is 250MPa, and the jet speed is 500m/s;
(2) Mixing the vesicle solution obtained in the step (1) with the chlorella composition, the lactobacillus/algae extract fermentation product and the plant extract at 40 ℃, and uniformly carrying out high-pressure microjet treatment to obtain the skin care composition containing the lactobacillus/algae extract fermentation product, wherein the pressure of the high-pressure microjet treatment is 300MPa, and the jet speed is 650m/s.
Example 4
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that the formulation does not include the extract of nannochloropsis oculata, the reduction amount of which is complemented by the extract of nannochloropsis oculata, and the remaining components and addition amounts are the same as those of example 1, and the preparation method is referred to in example 1.
Example 5
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that the formulation does not include a very small amount of chlorella extract, the balance of components and amounts added being the same as example 1, the preparation being as described in example 1.
Example 6
This example provides a skin care composition containing lactobacillus/algae extract fermentation product, which is different from example 1 only in that the plant extract does not include coconut fruit extract, the reduced amount thereof is distributed into the calamus root extract and myrrh resin extract in a part ratio, and the remaining components and the added amounts are the same as those of example 1, and the preparation method is referred to example 1.
Example 7
This example provides a skin care composition containing lactobacillus/algae extract fermentation product, which is different from example 1 only in that the plant extract does not include calamus root extract, the reduced amount thereof is distributed into coconut fruit extract and myrrh resin extract in a part ratio, and the remaining components and the added amounts are the same as those of example 1, and the preparation method is referred to example 1.
Example 8
This example provides a skin care composition containing lactobacillus/algae extract fermentation product, which is different from example 1 only in that the plant extract does not include myrrh resin extract, the reduced amount thereof is distributed into coconut fruit extract and calamus root extract in a part ratio, and the remaining components and the added amounts are the same as those of example 1, and the preparation method is referred to example 1.
Example 9
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that the formulation does not include skin conditioning agent, the reduction amount is complemented by deionized water, and the remaining components and addition amounts are consistent with example 1, and the preparation method is referred to in example 1.
Example 10
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that isononyl isononanoate is not included in the skin conditioner, the reduced amounts are distributed in parts ratio to ascorbic acid, lecithin and squalane, the remaining components and added amounts are the same as in example 1, and the preparation method is referred to in example 1.
Example 11
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that the skin conditioning agent does not include ascorbic acid, the reduced amount of which is distributed in parts ratio into isononyl isononanoate, lecithin and squalane, the remaining components and the added amounts being in accordance with example 1, the preparation method being referred to example 1.
Example 12
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that the skin conditioning agent does not include lecithin, the reduced amount of which is distributed in parts ratio into isononyl isononanoate, ascorbic acid and squalane, the remaining components and the added amounts being in accordance with example 1, the preparation method being referred to example 1.
Example 13
This example provides a skin care composition comprising lactobacillus/algae extract fermentation product which differs from example 1 only in that squalane is not included in the skin conditioner, the reduced amounts thereof are distributed in parts ratio to isononyl isononanoate, ascorbic acid and lecithin, the remaining components and added amounts are the same as in example 1, and the preparation method is referred to in example 1.
Comparative example 1
This comparative example provides a skin care composition comprising lactobacillus/algae extract fermentation product, which differs from example 1 only in that the formulation does not include chlorella composition, the reduced amount of which is distributed to each component of lactobacillus/algae extract fermentation product, plant extract and fat in parts ratio, and the remaining components and added amounts are the same as in example 1, and the preparation method is referred to in example 1.
Comparative example 2
This comparative example provides a skin care composition comprising lactobacillus/algae extract fermentation product, which differs from example 1 only in that the formulation does not include plant extract, the reduced amount of which is distributed in parts ratio to the components of lactobacillus/algae extract fermentation product, chlorella composition and fat, the remaining components and the added amounts are the same as in example 1, and the preparation method is referred to in example 1.
Comparative example 3
This comparative example provides a skin care composition comprising lactobacillus/algae extract fermentation product, which is different from example 1 only in that no fat is included in the formulation, the reduced amount of which is allocated to each component of lactobacillus/algae extract fermentation product, chlorella composition and plant extract in parts ratio, and the remaining components and added amounts are identical to example 1, and the preparation method is referred to example 1.
Comparative example 4
This comparative example provides a skin care composition differing from example 1 only in that the formulation does not include lactobacillus/algae extract fermentation product, the reduced amount of which is apportioned to the components of the chlorella composition, plant extract and grease in parts, the remaining components and added amounts being identical to example 1, the preparation method being as described in example 1.
Experimental example 1 erythrocyte hemolysis experiment
The erythrocyte hemolysis test (Red blood cell haemolysis test, RBC) is one of the alternative methods for the rabbit eye-stimulating test, and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of cellular hemoglobin and the degree of denaturation. RBC experiments can be used to evaluate eye irritation of cosmetics.
The samples of examples 1-13 were subjected to erythrocyte hemolysis experiments. The experiment adopts the RBC experiment test method and the judgment standard of the European alternative method verification center, the specific judgment standard is shown in the table 2, wherein HD 50 is the sample concentration when 50% of red blood cells are hemolyzed, DI is the protein denaturation index, and L/D is the ratio of HD 50 to DI.
TABLE 1
The results of the irritation of each of the samples to be tested prepared in examples 1 to 13 are shown in Table 2.
TABLE 2
| Sample of | L/D | Grading |
| Example 1 | >100 | No irritation |
| Example 2 | >100 | No irritation |
| Example 3 | >100 | No irritation |
| Example 4 | >100 | No irritation |
| Example 5 | >100 | No irritation |
| Example 6 | >100 | No irritation |
| Example 7 | >100 | No irritation |
| Example 8 | >100 | No irritation |
| Example 9 | >100 | No irritation |
| Example 10 | >100 | No irritation |
| Example 11 | >100 | No irritation |
| Example 12 | >100 | No irritation |
| Example 13 | >100 | No irritation |
From the results of the irritation of the samples in Table 2, the compositions prepared in examples 1-13 of the present invention were classified as non-irritating, indicating that the skin care compositions of the present invention all have mild and non-irritating characteristics.
Test example 2 antioxidant efficacy study-DPPH free radical scavenging experiment
Taking 1g of samples of examples 1-13 and comparative examples 1-4, adding 2mL of absolute ethyl alcohol, adding 2mL of 60 mu mol/L DPPH solution after complete dissolution, mixing uniformly, standing for 30min, zeroing by absolute ethyl alcohol, measuring absorbance at 517nm as Ai, mixing 2mL of absolute ethyl alcohol solvent and 2mL of DPPH solution uniformly to measure absorbance as Ac, dissolving 1g of composition matrix, adding 2mL of absolute ethyl alcohol solvent, mixing uniformly, and measuring absorbance as Aj.
The radical scavenging rate was calculated as follows.
Clearance (%) = [1- (Ai-Aj)/Ac ] ×100%
Wherein, the Aj reaction sample itself contributes to absorbance, the Ai sample acts on the absorbance value after DPPH, and Ac is the absorption of DPPH itself at the measurement wavelength.
The DPPH free radical scavenging rate reflects the antioxidant capacity to a certain extent, and the higher the scavenging rate is, the better the antioxidant degree is, and the better the anti-aging effect is, so the anti-aging effect can be judged through the research of the DPPH free radical scavenging capacity of the composition. The results are shown in table 3 (5 replicates per group of samples, data presented as average).
TABLE 3 Table 3
As can be seen from Table 3, the combination prepared in examples 1-3 has a better DPPH scavenging effect, the scavenging rate is more than 86%, and comparison of examples 1 and comparative examples 1-4 shows that the lactobacillus/algae extract fermentation product, the chlorella composition, the plant extract and the grease in the skin care composition have a synergistic effect on the antioxidation effect, and the synergistic cooperation of the four can lead to better antioxidation effect of the product, and the addition and the type selection of the skin conditioning agent have better antioxidation performance on the product.
Test example 3
Propionibacterium acnes inhibition assay:
The samples prepared in examples 1, 6-8 and comparative examples 1-4 were assayed for their bacteriostatic effect against propionibacterium acnes. Preparing culture medium, namely weighing 33g of lecithin-Tween nutrient agar culture medium and 29.3g of liquid thioglycolate culture medium, adding 1L of ultrapure water, stirring, heating, boiling to dissolve completely, sub-packaging, sterilizing at 121 ℃ for 30min under high pressure, cooling to 60 ℃, and preserving heat in a water bath kettle for later use.
Resuscitator and bacterial suspension of Propionibacterium acnes are prepared by taking freeze-dried powder of Propionibacterium acnes, fusing ampoule bottles under an alcohol lamp, adding 1mL of 0.85% physiological saline, stirring uniformly, inoculating on 3 plates containing culture medium, and culturing for 3 days under anaerobic and 37 ℃ conditions. The slant colonies were scraped with an inoculating loop, and the bacterial suspension was diluted with physiological saline to a concentration of 10 6CFU·mL-1. Under aseptic operation, the bacterial suspension is taken and uniformly smeared on a culture medium by a coating rod. The oxford cup is lightly placed on the surface of the solidified culture medium by using sterile forceps, and the oxford cup (with the outer diameter of 8 mm) is lightly pressed to ensure good contact. 200 mu L of the solution is added into an oxford cup, deionized water is added as a blank control, the solution is cultured for 48 hours in a 37 ℃ incubator and taken out, and the diameter of an effective inhibition zone of the test solution in each culture dish is measured. Each set of experiments was repeated 3 times, twice each time to perform the test.
The judgment standard is that the diameter of the inhibition zone is 20mm, the diameter of the inhibition zone is 10-20mm, the diameter of the inhibition zone is medium, the diameter of the inhibition zone is <10mm, the inhibition is not performed, and the results are shown in Table 4:
TABLE 4 Table 4
| Group of | Average inhibition zone diameter (mm) |
| Example 1 | 24.96 |
| Example 6 | 12.96 |
| Example 7 | 14.18 |
| Example 8 | 13.42 |
| Comparative example 1 | 14.71 |
| Comparative example 2 | 8.39 |
| Comparative example 3 | 13.28 |
| Comparative example 4 | 7.48 |
| Blank control group | 4.81 |
As shown in the data of Table 4, the composition provided by the invention has good inhibition effect on Propionibacterium acnes, the products prepared in comparative examples 1-4 also have a certain inhibition effect compared with a blank control group, and three components of lactobacillus/algae extract fermentation product, chlorella composition and plant extract in the composition have a certain synergistic effect on the effects, so that the composition has a remarkable inhibition effect on Propionibacterium acnes, effectively controls the propagation of Propionibacterium acnes, maintains the ecological balance of skin colonies, and the type selection of the plant composition has a great influence on the inhibition effect of Propionibacterium acnes.
Test example 4 evaluation of moisturizing Effect
The samples of examples 1 to 5 and comparative examples 1 to 4 were evaluated for long-lasting moisturizing effect and skin-tightening effect:
90 testers, 20-45 years old, were randomly selected and divided into 9 groups of 10. The samples obtained in examples 1-5 and comparative examples 1-4 were used by the 9 groups of testers, once a day after cleansing the face in the morning and evening, in the same amount for 30 days, during which no other skin care products were used.
Skin moisture content was measured with a skin moisture content tester Corneometer CM825 on day 0 and day 30, respectively, and the moisture content of the skin before and after the use of the samples was evaluated, and the skin moisture content change rate = (skin moisture content after 30 days of use-skin moisture content before use)/skin moisture content before use x 100%, and the test results are shown in table 5.
TABLE 5
| Group of | Skin moisture% change rate |
| Example 1 | 37.16 |
| Example 2 | 36.52 |
| Example 3 | 37.43 |
| Example 4 | 31.19 |
| Example 5 | 30.42 |
| Comparative example 1 | 28.19 |
| Comparative example 2 | 27.81 |
| Comparative example 3 | 23.53 |
| Comparative example 4 | 24.16 |
As can be seen from Table 5, the skin care compositions and emulsions of the present invention have a good long-lasting moisturizing effect, wherein the lactobacillus/algae extract fermentation product, the chlorella composition, the plant extract and the oil promote each other in the effect, and have unexpected synergy. And the choice of the type of the chlorella composition also has an important influence on the moisture retention of the product.
The applicant states that the present invention is illustrated by the above examples as a skin care composition comprising lactobacillus/algae extract fermentation product, and a method for preparing the same and application thereof, but the present invention is not limited to the above examples, i.e. it is not meant that the present invention must be practiced by relying on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (18)
1. A skin care composition comprising a lactobacillus/algae extract fermentation product, characterized in that the skin care composition comprising a lactobacillus/algae extract fermentation product, a chlorella composition, a plant extract, a grease, an emulsifier, a humectant, a skin conditioner and/or a preservative;
The chlorella composition comprises an ocular vein micro-parachloropsis extract and a very small chlorella extract, wherein the mass ratio of the ocular vein micro-parachloropsis extract to the very small chlorella extract is 1 (0.5-10);
The plant extract is a combination of coconut fruit extract, calamus root extract and myrrh resin extract, wherein the mass ratio of the coconut fruit extract to the calamus root extract to the myrrh resin extract is (3-5): 2-4): 1-5;
the skin conditioning agent comprises a combination of isononyl isononanoate, ascorbic acid, lecithin and squalane;
The grease is a combination of butter tree fruit grease, macadimia nut seed oil, olive fruit oil and argania spinosa kernel oil, and the mass ratio of the butter tree fruit grease, the macadimia nut seed oil, the olive fruit oil and the argania spinosa kernel oil is (1-3): 3-5): 2-4): 1-5.
2. The skin care composition comprising lactobacillus/algae extract fermentation product according to claim 1, wherein the skin care composition comprising lactobacillus/algae extract fermentation product comprises 0.5 to 20 parts of lactobacillus/algae extract fermentation product, 1 to 10 parts of chlorella composition, 1 to 15 parts of plant extract, 1 to 10 parts of grease, 1 to 10 parts of emulsifier, 1 to 10 parts of humectant, 1 to 10 parts of skin conditioner and 0.1 to 1 part of preservative.
3. The skin care composition comprising lactobacillus/algae extract fermentation product according to claim 2, wherein the skin care composition comprising lactobacillus/algae extract fermentation product comprises 3-15 parts of lactobacillus/algae extract fermentation product, 2-9 parts of chlorella composition, 2-12 parts of plant extract, 2-10 parts of grease, 3-9 parts of emulsifier, 2-9 parts of humectant, 1-10 parts of skin conditioner and 0.1-1 part of preservative.
4. The skin care composition comprising lactobacillus/algae extract fermentation product according to claim 3, wherein the skin care composition comprising lactobacillus/algae extract fermentation product comprises 5-12 parts of lactobacillus/algae extract fermentation product, 3-8 parts of chlorella composition, 3-10 parts of plant extract, 3-9 parts of grease, 3-7 parts of emulsifier, 2-8 parts of humectant, 1-10 parts of skin conditioner and 0.1-1 part of preservative.
5. The lactobacillus/algae extract fermentation product containing skin care composition of claim 1, wherein the humectant comprises any one or a combination of at least two of sodium polyglutamate, ceramide, butanediol, or 1, 2-hexanediol.
6. The lactobacillus/algae extract fermentation product containing skin care composition of claim 5, wherein the humectant comprises a combination of sodium polyglutamate, ceramide, butanediol, and 1, 2-hexanediol.
7. The skin care composition comprising a lactobacillus/algae extract fermentation product according to claim 1, wherein the emulsifier comprises PEG-8C 12-20 acid ester and/or potassium cetyl phosphate.
8. The skin care composition comprising a lactobacillus/algae extract fermentation product according to claim 7, wherein the emulsifier is a combination of PEG-8C 12-20 acid ester and potassium cetyl phosphate.
9. The skin care composition comprising fermentation product of lactobacillus/algae extract according to claim 1, wherein the mass ratio of isononyl isononanoate, ascorbic acid, lecithin and squalane is (1-5): 0.5-2.5): 1-2.5.
10. The skin care composition comprising a lactobacillus/algae extract fermentation product of claim 1, wherein the preservative comprises p-hydroxyacetophenone.
11. A method of preparing a skin care composition comprising a lactobacillus/algae extract fermentation product according to any of claims 1 to 10, wherein the method of preparing comprises:
(1) Mixing an emulsifier, a humectant, grease, deionized water, a skin conditioning agent and/or a preservative, and carrying out high-pressure microjet treatment to obtain a vesicle solution;
(2) Mixing the vesicle solution obtained in the step (1) with a chlorella composition, lactobacillus/algae extract fermentation products and plant extracts, and carrying out high-pressure micro-jet treatment to obtain the skin care composition containing the lactobacillus/algae extract fermentation products.
12. The method of claim 11, wherein the temperature of the mixing in step (1) is 45-55 ℃.
13. The method according to claim 11, wherein the high-pressure micro-jet treatment in step (1) is performed at a pressure of 200 to 300MPa and a jet velocity of 500 to 700m/s.
14. The method of claim 11, wherein the temperature of the mixing in step (2) is 40-50 ℃.
15. The method according to claim 11, wherein the high-pressure micro-jet treatment in step (2) is performed at a pressure of 200 to 300MPa and a jet velocity of 500 to 700m/s.
16. Use of a skin care composition comprising a lactobacillus/algae extract fermentation product according to any of claims 1 to 10 for the preparation of a cosmetic.
17. The use according to claim 16, wherein the cosmetic comprises a lotion, a cream, a face wash or a mask.
18. The use according to claim 16, wherein the skin care composition comprising lactobacillus/algae extract fermentation product is present in the cosmetic in a mass percentage of 0.1-15%.
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