CN121027556A

CN121027556A - A device for detecting five-part differential hematology cells and MxA to identify viral infections.

Info

Publication number: CN121027556A
Application number: CN202511194128.0A
Authority: CN
Inventors: 刘朝风; 邓哲; 刘铭秋; 黄洲
Original assignee: Nanjing Kerui Biomedical Technology Co ltd
Current assignee: Nanjing Kerui Biomedical Technology Co ltd
Priority date: 2025-08-25
Filing date: 2025-08-25
Publication date: 2025-11-28

Abstract

The application discloses a device for detecting five-class blood cells and MxA to identify viral infection. The device comprises a DIFF reaction detection system, an MxA immunofluorescence detection system, a sample collection system, a first DIFF reagent pipeline and a second DIFF reagent pipeline, wherein the collected sample is conveyed to the DIFF reaction detection system through the sample collection system, the first DIFF reagent pipeline guides a first DIFF reagent in a first DIFF reagent pool to the DIFF reaction detection system, the second DIFF reagent pipeline guides a second DIFF reagent in a second DIFF reagent pool to the DIFF reaction detection system, so that the DIFF reaction detection is completed in the DIFF reaction detection system, the collected sample is conveyed to the MxA immunofluorescence detection system through the sample collection system, the first pushing unit pushes an MxA reagent card at the uppermost layer of the MxA reagent card stacking unit to the conveying unit, and the conveying unit is used for conveying the MxA reagent card to sequentially pass through the light measurement and incubation unit and the reading unit.

Description

Device for detecting five-class blood cells and MxA to identify viral infection

Technical Field

The application relates to the technical field of medical equipment, in particular to a device for detecting five-class blood cells and MxA to identify viral infection.

Background

In the related art, five-class detection of blood cells and immunofluorescence detection of MxA are usually performed by two independent devices, and a user is required to manually move the MxA reagent card strip, so that not only is the number of times of patient detection high, but also the detection efficiency is relatively low.

Disclosure of Invention

The embodiment of the application provides a device for detecting five-class blood cells and MxA to identify viral infection, which can simultaneously detect five-class blood cells and the immunofluorescence of MxA and can give consideration to the detection efficiency of immunofluorescence.

The embodiment of the application provides a device for detecting five-class blood cells and MxA to identify viral infection, which comprises:

DIFF reaction detection system;

the MxA immunofluorescence detection system comprises a first pushing unit, an MxA reagent card strip stacking unit, a conveying unit, a photometry and incubation unit and a reading unit, wherein the MxA reagent card strip stacking unit is used for stacking MxA reagent card strips, the first pushing unit is used for pushing the MxA reagent card strip at the uppermost layer of the MxA reagent card strip stacking unit to the conveying unit, and the conveying unit is used for conveying the MxA reagent card strips to sequentially pass through the photometry and incubation unit and the reading unit;

The sample collection system is used for conveying the collected sample to the DIFF reaction detection system or the MxA immunofluorescence detection system;

A first DIFF reagent line communicating with a first DIFF reagent tank and with the DIFF reaction detection system to direct a first DIFF reagent within the first DIFF reagent tank to the DIFF reaction detection system;

And the second DIFF reagent pipeline is used for communicating with a second DIFF reagent pool and communicating with the DIFF reaction detection system so as to guide the second DIFF reagent in the second DIFF reagent pool to the DIFF reaction detection system.

In some of these embodiments, the MxA reagent card strip stacking unit for detecting MxA comprises:

the device comprises a stand, a storage bin and a control unit, wherein the stand is provided with a storage bin for accommodating MxA reagent clamping strips, and a discharge hole is formed in the upper surface of the stand by the storage bin;

the bottom plate is arranged at the bottom of the vertical frame and is provided with a through hole communicated with the storage bin;

The ejection mechanism is arranged below the bottom plate and comprises ejection pieces which are vertically and movably arranged so as to extend into the storage bin through the through holes and eject the MxA reagent clamping strips at the uppermost layer out of the storage bin;

the first pushing unit is used for pushing the MxA reagent card strip positioned at the uppermost layer outside the storage bin to the conveying unit.

In some of these embodiments, the MxA reagent card strip stacking unit comprises:

a mounting plate;

The plurality of stand frames are distributed on the mounting plate along the circumferential direction of the mounting plate;

a plurality of bottom plates which are arranged at the bottoms of the plurality of vertical frames in a one-to-one correspondence manner, and

And the rotating mechanism rotates with the mounting plate to drive the mounting plate to rotate around the axis of the mounting plate.

In some of these embodiments, the device for detecting five-class blood cells and MxA to identify a viral infection further comprises:

an HGB detection system comprising a WBC cell, an RBC cell, and a negative pressure chamber in communication with the WBC cell and the RBC cell, respectively, and

The WBC reagent pipeline is used for communicating with the WBC reagent pool and the WBC pool so as to guide the WBC reagent in the WBC reagent pool to the WBC pool;

Wherein the sample collection system is further configured to deliver collected samples to the WBC and RBC reservoirs.

In some of these embodiments, the device for detecting five-class blood cells and MxA to identify a viral infection further comprises a waste collection system in communication with the WBC pool, the RBC pool, the negative pressure chamber, and the DIFF reaction detection system, respectively.

In some embodiments, the device for detecting five-class blood cells and MxA to identify viral infection further comprises a diluent delivery line having a dispensing three-way valve with one dispensing inlet for communication with a diluent reservoir and two dispensing outlets in communication with the WBC reservoir and the RBC reservoir, respectively.

In some embodiments, the diluent conveying pipeline is provided with a first three-way valve and a first injection unit, one interface of the first three-way valve is used for being communicated with the diluent pool, the other two interfaces of the first three-way valve are respectively communicated with the first injection unit and the distribution inlet, and the first injection unit is used for guiding diluent in the diluent pool into the distribution three-way valve.

In some embodiments, the sample collection system comprises a second three-way valve, a second injection unit, and a sample collection unit, wherein one interface on the second three-way valve is used for communicating with a dilution liquid pool, and the other two interfaces on the second three-way valve are respectively connected with the second injection unit and the sample collection unit.

In some embodiments, a third three-way valve and a third injection unit are arranged on the first DIFF reagent pipeline, one interface on the third three-way valve is used for being communicated with the first DIFF reagent tank, the other two interfaces on the third three-way valve are respectively communicated with the third injection unit and the DIFF reaction detection system, and the third injection unit is used for introducing the first DIFF reagent in the first DIFF reagent tank into the DIFF reaction detection system;

And/or a fourth three-way valve and a fourth injection unit are arranged on the second DIFF reagent pipeline, one interface on the fourth three-way valve is used for being communicated with the second DIFF reagent tank, the other two interfaces on the fourth three-way valve are respectively communicated with the fourth injection unit and the DIFF reaction detection system, and the fourth injection unit is used for guiding the second DIFF reagent in the second DIFF reagent tank into the DIFF reaction detection system.

In some of these embodiments, the DIFF reaction detection system includes a DIFF reaction detection unit including a reaction zone and a detection zone, a fifth injection unit in communication with the reaction zone, and a sixth injection unit in communication with the detection zone, the fifth injection unit for providing motive force to push a sample on the reaction zone into the detection zone, the sixth injection unit for providing sheath fluid into the detection zone.

The device for detecting five-class blood cells and MxA to identify viral infection comprises a DIFF reaction detection system, an MxA immunofluorescence detection system, a sample collection system, a first DIFF reagent pipeline and a second DIFF reagent pipeline, wherein the collected sample is conveyed to the DIFF reaction detection system through the sample collection system, the first DIFF reagent pipeline guides a first DIFF reagent in a first DIFF reagent pool to the DIFF reaction detection system, the second DIFF reagent pipeline guides a second DIFF reagent in a second DIFF reagent pool to the DIFF reaction detection system, so that the DIFF reaction detection is completed in the DIFF reaction detection system, the collected sample is conveyed to the MxA immunofluorescence detection system through the sample collection system, in the MxA immunofluorescence detection system, a first pushing unit pushes an MxA reagent card on the uppermost layer of an MxA reagent card stacking unit to a conveying unit, and the conveying unit is used for conveying the MxA reagent card to pass through a light measuring unit, an incubation unit and a reading unit in sequence, so that the detection of the content of MxA is completed rapidly.

Drawings

In order to more clearly illustrate the embodiments of the application or the technical solutions in the prior art, the drawings that are necessary for the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the application and that other drawings may be obtained from them without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of an apparatus for detecting five-class blood cells and MxA to identify viral infection according to an embodiment of the present application;

FIG. 2 is a schematic structural diagram of an MxA reagent card strip stacking unit according to an embodiment of the present application;

fig. 3 is a schematic structural diagram of an MxA immunofluorescence detection system according to an embodiment of the present application.

Reference numerals in the drawings:

100. the system comprises a DIFF reaction detection system, 110, a DIFF reaction detection unit, 120, a fifth injection unit, 130, and a sixth injection unit;

200. the device comprises an MxA immunofluorescence detection system, 210, a first pushing unit, 220, an MxA reagent card strip stacking unit, 221, a vertical frame, 221a, a bin, 221b, a discharge hole, 222, a bottom plate, 223, a material ejection mechanism, 224, a mounting plate, 225, a rotating mechanism, 230, a conveying unit, 240, a photometry and incubation unit and 250, a reading unit;

300. A sample collection system; 310, a second three-way valve, 320, a second injection unit, 330, a sample acquisition unit;

400. a first DIFF reagent line 410, a third three-way valve 420, a third injection unit;

500. a second DIFF reagent line 510, a fourth three-way valve 520, a fourth injection unit;

600. HGB detection system, 610, WBC pool, 620, RBC pool, 630, negative pressure chamber;

700. WBC reagent lines;

800. a waste liquid collection system;

900. A diluent delivery line 910, a first three-way valve 920, a first injection unit 930, a dispensing three-way valve;

10. A dilution liquid pool, 20, a first DIFF reagent pool, 30, a second DIFF reagent pool and 40, a WBC reagent pool.

Detailed Description

The present application will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present application more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application.

In the prior art, five-class detection of blood cells and immunofluorescence detection of MxA are usually performed by two independent devices, and a user is required to manually move the MxA reagent card strip, so that not only is the number of times of patient detection more, but also the detection efficiency is relatively low.

In order to solve the above-mentioned problems, referring to fig. 1-2, the present application provides a device for detecting five-class blood cells and MxA to identify viral infection, which comprises a DIFF (DIFFERENTIAL LEUKOCYTE COUNT, white blood cell class count) reaction detection system 100, an MxA immunofluorescence detection system 200, a sample collection system 300, a first DIFF reagent line 400 and a second DIFF reagent line 500.

The DIFF response detection system 100 evaluates the type of infection, immune status, and blood disease risk by five differential counts of leukocytes (neutrophils, lymphocytes, monocytes, eosinophils, basophils) by flow cytometry or cytochemical staining techniques.

The MxA immunofluorescence detection system 200 for detecting MxA (Myxovirus resistance protein A, a myxovirus-resistant protein a) includes a first pushing unit 210, an MxA reagent card strip stacking unit 220, a transmission unit 230, a photometry and incubation unit 240, and a reading unit 250, the MxA reagent card strip stacking unit 220 is used for stacking MxA reagent card strips, the first pushing unit 210 is used for pushing the MxA reagent card strip at the uppermost layer of the MxA reagent card strip stacking unit 220 to the transmission unit 230, the transmission unit 230 is used for transmitting the MxA reagent card strip sequentially through the photometry and incubation unit 240 and the reading unit 250;

The MxA reagent card strip stacking unit 220 is capable of stacking a plurality of MxA reagent card strips together, preloading a large number of MxA reagent card strips, and vertically stacking in order. The uppermost MxA reagent card strip is in a standby state and can be pushed at any time. The first pushing unit 210 pushes out the uppermost MxA reagent card strip horizontally to the starting position of the conveying unit 230 by using a mechanical arm or a linear sliding table, and the MxA reagent card strip enters the starting point of the detection process.

The conveying unit 230 may drive the conveying belt or the rotary table by using a stepping motor, so as to drive the MxA reagent card strip to sequentially pass through the light measuring and incubation unit 240 and the reading unit 250, and the conveying unit 230 may be equipped with a sensor to monitor the position of the MxA reagent card strip, so as to ensure accurate positioning.

In the photometry and incubation unit 240, it is usually performed in a dark room with a certain temperature, for example, the temperature is controlled at 37 ℃, the environment required for the reaction is provided, the incubation time (for example, 10-15 minutes) is set, and the adjustment is realized by a timer or a transmission speed. In a darkroom, a fluorescence excitation light source (such as an LED) and a photoelectric sensor are integrated, a card strip detection line (T line) and a control line (C line) are scanned, and fluorescence intensity data are collected in real time for quantitative analysis.

In the reading unit 250, the result is determined by the code scanner, i.e. the visual recognition system, and the result is determined by the instrument algorithm, and finally a report is output.

The whole process of feeding from the clamping strip to the result output is automatic, continuous sample processing is supported, the conveying channel and the single-use clamping strip can be closed, cross contamination between samples is avoided, and in addition, the clamping strips of different projects can be replaced, so that the multi-scene requirements are met.

The sample collection system 300 is used to transfer the collected sample to the DIFF reaction detection system 100 or the MxA immunofluorescence detection system 200, and it should be understood that when the sample collection system 300 transfers the collected sample to the MxA immunofluorescence detection system 200, the sample is mainly transferred to the MxA reagent card strip of the uppermost volume, and specifically, the transfer may be completed at the MxA reagent card strip stacking unit 220 or may be completed at the initial position of the transfer unit 230.

The first DIFF reagent line 400 is used to communicate with the first DIFF reagent tank 20 and with the DIFF reaction detection system 100 to direct the first DIFF reagent in the first DIFF reagent tank 20 to the DIFF reaction detection system 100, and the second DIFF reagent line 500 is used to communicate with the second DIFF reagent tank 30 and with the DIFF reaction detection system 100 to direct the second DIFF reagent in the second DIFF reagent tank 30 to the DIFF reaction detection system 100.

The first DIFF reagent pool 20 and the second DIFF reagent pool 30 can be respectively loaded with different reagents (such as hemolysis agent and staining agent), and support complex detection flow, wherein the first DIFF reagent pool 20 and the second DIFF reagent pool 30 are in bottle-packed structures, so that reagents can be automatically selected according to detection items, manual replacement of reagent bottles is avoided, and detection continuity is improved.

In the embodiment of the application, the blood cell analysis and the immune detection of the MxA are integrated on the same platform, the five-class blood cell analysis and the immune fluorescence detection of the MxA can be simultaneously completed by the same sample, repeated sampling and split charging are avoided, the waiting time of a patient is reduced, the DIFF reaction detection system 100 and the MxA immune fluorescence detection system 200 independently operate, synchronous detection of blood routine and an immune project of the MxA is supported, and the report turnaround time is shortened.

The device for detecting the five-class blood cells and the MxA to identify the viral infection can realize the identification of the viral infection more quickly and accurately, and can realize the detection of the viral infection and the degree of the viral infection of an individual by taking the blood sample of the individual in one time and less quantity.

In some embodiments, referring to fig. 2, the mxa reagent card strip stacking unit 220 includes a stand 221, a bottom plate 222, and a ejection mechanism 223.

The stand 221 can be formed with a feed bin 221a for accommodating MxA reagent card strips, the feed bin 221a forms a discharge hole 221b on the upper surface of the stand 221, the feed bin 221a is used as a storage container of MxA reagent card strips, the feed bin 221a limits the stacking direction of the card strips through the stand 221 structure, and the vertical arrangement of the card strips is ensured. The top outlet 221b is designed to allow only a single MxA reagent card strip to pass through, preventing multiple MxA reagent card strips from being ejected simultaneously.

The bottom plate 222 is arranged at the bottom of the vertical frame 221, through holes communicated with the bin 221a are formed in the bottom plate 222, the bottom plate 222 supports stacked MxA reagent clamping strips, and the through holes serve as channels of ejection pieces. The position is usually located at the bottom center of the bin 221a, so that the acting force of the ejection part is uniformly distributed, and the inclination of the clamping strips is avoided.

The ejection mechanism 223 is arranged below the bottom plate 222, the ejection mechanism 223 comprises ejection pieces which are vertically movably arranged so as to extend into the storage bin 221a through the through holes, and the uppermost MxA reagent card strip is ejected out of the storage bin 221a and is controlled to reciprocate along the vertical direction through a motor, a cylinder or electromagnetic drive. When the ejection part ascends, the MxA reagent card strip at the bottommost layer is supported, the jacking height can be controlled by a sensor, the reset is ensured after only a single MxA reagent card strip is ejected, and the rest MxA reagent card strips fall back to the bottom plate 222 due to gravity to wait for the next operation.

The first pushing unit 210 is configured to push the MxA reagent card strip located at the uppermost layer outside the bin 221a to the conveying unit 230, and after the MxA reagent card strip is ejected out of the bin 221a, the MxA reagent card strip is laterally transferred to the conveying unit 230 through a push rod, a rotary table or a vacuum nozzle, and the pushing action and the resetting of the ejection mechanism 223 are synchronously performed, so as to avoid blocking caused by retention of the MxA reagent card strip at the discharge hole 221 b.

Therefore, the clamping strip can be supplied just by the time without manual intervention, continuous sample processing is supported, the detection flux is obviously improved, the mechanical limit and the sensor are cooperatively controlled, only a single Zhang Katiao is ejected each time, the adhesion or the empty pushing of a plurality of sheets is avoided, and the system fault rate is reduced.

Still further, referring to fig. 2, the mxa reagent card strip stacking unit 220 includes a mounting plate 224, a plurality of stand frames 221, a plurality of bottom plates 222 and a rotation mechanism 225, wherein the plurality of stand frames 221 are distributed on the mounting plate 224 along a circumferential direction of the mounting plate 224, the plurality of bottom plates 222 are disposed at bottoms of the plurality of stand frames 221 in a one-to-one correspondence, and the rotation mechanism 225 rotates with the mounting plate 224 to drive the mounting plate 224 to rotate around an axis of the mounting plate 224.

The stand 221 distributed along the circumference of the mounting plate 224 forms independent bins 221a, each bin 221a can store one or more MxA reagent card strips, the number of the stand 221 can be increased or decreased (e.g. 4/6/8) according to the requirement, and the parallel detection of multiple items is supported.

It should be appreciated that the ejector mechanism 223 is disposed below the mounting plate 224, so that the mounting plate 224 is provided with a relief hole in communication with the through hole of the bottom plate 22, so that the ejector member can extend into the bin 221a through the relief hole and the through hole.

The station switching of the stand 221 is realized by driving the mounting plate 224 to rotate around the axis through a stepping motor or a servo motor. The encoder or photosensor ensures positioning accuracy (e.g., ±0.1°). When the satisfactory chromatographic card strip of one of the uprights 221 is about to run out, the system automatically rotates to the next upright 221, and 'seamless' feeding is realized.

The multi-stand 221 design supports batch loading, and in combination with the rotary mechanism 225, enables "rotary feeding" to be achieved, thereby enabling non-stop feeding. And the parallel of multiple projects is supported, for example, the combined detection of the infection markers does not need to stop and change cards, and the annular layout can enable the whole device to be designed in a miniaturized way.

In some of these embodiments, the apparatus for detecting five-class Blood cells and MxA to identify a viral infection further comprises an HGB detection system 600 and a WBC reagent line 700, the HGB detection system 600 including a WBC (White Blood Cell) pool, a RBC (Red Blood Cell) pool, and a negative pressure chamber 630, the negative pressure chamber 630 in communication with the WBC pool 610 and the RBC pool 620, respectively, the WBC reagent line 700 for communicating with the WBC reagent pool 40 and with the WBC pool 610 for directing WBC reagent within the WBC reagent pool 40 to the WBC pool 610, wherein the sample collection system 300 is further for delivering collected samples to the WBC pool 610 and the RBC pool 620.

The WBC cell 610 receives a portion of the white blood cells in the sample, reacts with WBC reagents (e.g., red blood cells lyse), releases the white blood cells for subsequent sorting and counting, and the cell body may incorporate a stirring mechanism or ultrasonic vibration to ensure adequate mixing of the reagents with the sample.

RBC cell 620 directly analyzes parameters related to red blood cells (e.g., hemoglobin concentration, hematocrit) and can be detected spectrophotometrically or impedance, with the independent cell body avoiding interference of leukocyte lysis with red blood cell detection.

The WBC reagent pipeline 700 is used for accurately conveying the hemolytic agent (such as quaternary ammonium salt) or the coloring agent (such as fluorescent dye) in the WBC reagent tank 40 to the WBC tank 610, and a three-way valve and an electric injection unit can be arranged on the WBC reagent pipeline 700, so that the accurate suction of the WBC reagent tank can be realized through the three-way valve and the electric injection unit, and the detection accuracy is ensured.

The negative pressure chamber 630 generates negative pressure through the vacuum pump, so that the sample and the reagent are driven to flow between the tanks, a mechanical pump is not needed, the fluid control is simplified, the cells in the WBC tank 610 and the RBC tank 620 are driven by the negative pressure to flow to the negative pressure chamber 630, each cell is counted in the flowing process, the measurement of the WBC and the RBC is completed (the light is irradiated to the blood sample reacted by the WBC reagent through the LED lamp, and then the HGB is calculated according to the light intensity received by the photoelectric receiver).

In the embodiment of the application, WBC and RBC detection are performed synchronously, shortening reporting time, and in addition, WBC cell 610 is independent of RBC cell 620, avoiding chemical interference of hemolytic agent on red blood cell detection.

Further, the device for detecting five-class blood cells and MxA to identify viral infection further includes a waste liquid collection system 800 in communication with the WBC cell 610, the RBC cell 620, the negative pressure chamber 630, and the DIFF reaction detection system 100, respectively.

The separate pipelines are respectively connected with the WBC pool 610, the RBC pool 620, the negative pressure chamber 630 and the DIFF reaction detection system 100, so that different sources of waste liquid are ensured to be separated and treated, the liquid level in the pool is monitored by the sensor, the waste liquid is automatically triggered to be discharged to the outside of the whole device, after the reaction of each pool is completed, the sensor sends a signal to the central controller, the valve in the waste liquid collection system 800 is opened, and the negative pressure drives the waste liquid to flow to the collection container in the waste liquid collection system 800.

It should be understood that, after the completion of the reaction and before the next reaction, the WBC tank 610 and the RBC tank 620 need to be cleaned to avoid residues, thereby affecting the next reaction result, so in this embodiment of the present application, referring to fig. 1, the apparatus for detecting five-class blood cells and MxA to identify viral infection further includes a diluent delivery pipeline 900, and the diluent delivery pipeline 900 is provided with a three-way valve 930, and the three-way valve 930 has one dispensing inlet and two dispensing outlets, the dispensing inlet is used for communicating with the diluent tank 10, and the two dispensing outlets are respectively correspondingly communicated with the WBC tank 610 and the RBC tank 620.

In the embodiment of the application, by adopting the distribution three-way valve 930, namely realizing double-tank liquid supply through a single valve, the number of pipeline joints is reduced, the leakage risk is reduced, and the whole length of the pipeline is reduced, so that the occupied space in the equipment is reduced, and the whole compact layout and the miniaturized design can be realized.

With reference to the foregoing description, in some embodiments of the present application, with continued reference to fig. 1, the diluent delivery pipeline 900 is provided with a first three-way valve 910 and a first injection unit 920, one port of the first three-way valve 910 is used for communicating with the diluent pool 10, and the other two ports of the first three-way valve 910 are respectively communicated with the first injection unit 920 and the dispensing inlet, and the first injection unit 920 is used for introducing the diluent in the diluent pool 10 into the dispensing three-way valve.

The first injection unit 920 is connected with a first interface of the first three-way valve 910, the dilution liquid tank 10 is connected with a second interface of the first three-way valve 910, a distribution inlet is connected with a third interface of the first three-way valve 910, the first interface is communicated with the second interface when the diluent is sucked, the first interface is not communicated with the third interface when the diluent is discharged, the first interface is not communicated with the second interface, the first interface is communicated with the third interface, the first three-way valve 910 is matched with the first injection unit 920, the accurate quantitative distribution of the diluent can be realized, and different detection parameter requirements are adapted.

It should be understood that the first injection unit 920 may be electrically operated, and the motor is used in combination with the ball screw to change the rotational motion into the linear motion, so as to facilitate precise control of the stroke of the piston in the first injection unit 920, thereby precisely controlling the suction and discharge amounts of the diluent.

Optionally, in an embodiment of the present application, the sample collection system 300 includes a second three-way valve 310, a second injection unit 320, and a sample collection unit 330, one interface on the second three-way valve 310 is used to communicate with the dilution liquid tank 10, and the other two interfaces on the second three-way valve 310 are respectively connected with the second injection unit 320 and the sample collection unit 330.

The second injection unit 320 is connected with the first interface of the second three-way valve 310, the dilution liquid tank 10 is connected with the second interface of the second three-way valve 310, the sample collection unit 330 is connected with the third interface of the second three-way valve 310, the second three-way valve 310 can also be an electromagnetic valve, automatic switching of communication objects is realized, when a sample is sucked, the first interface is conducted with the third interface, the second interface is in a closed state, when the dilution liquid in the dilution liquid tank 10 is sucked, the first interface is conducted with the second interface, the third interface is in a closed state, so that the dilution liquid and the sample can be mixed, and finally the mixture is discharged to the MxA immunofluorescence detection system 200 and the DIFF reaction detection system 100 through the third interface.

Optionally, the user may further directly draw the sample through the sample collection unit 330 and then discharge the sample through the third interface, that is, the sample is not required to be mixed, and after the second interface is used to draw the diluent in the diluent pool 10, the second interface is switched to the third interface, so that the diluent can be discharged through the sample collection unit 330, thereby cleaning the DIFF reaction detection system 100 and ensuring the detection result.

In this way, by the cooperation of the second injection unit 320 and the second three-way valve 310, the diluent and the sample can be accurately sucked, and the detection results of the MxA immunofluorescence detection system 200 and the DIFF reaction detection system 100 can be ensured.

It should be understood that the second injection unit 320 may be electrically powered, and the motor is used in combination with the ball screw to change the rotational motion into linear motion, so as to facilitate accurate control of the stroke of the piston in the second injection unit 320, thereby accurately controlling the amount of the sample and the diluent sucked.

Optionally, referring to fig. 1, a third three-way valve 410 and a third injection unit 420 are disposed on the first DIFF reagent pipeline 400, one port on the third three-way valve 410 is used for communicating with the first DIFF reagent tank 20, the other two ports on the third three-way valve 410 are respectively communicated with the third injection unit 420 and the DIFF reaction detection system 100, and the third injection unit 420 is used for introducing the first DIFF reagent in the first DIFF reagent tank 20 into the DIFF reaction detection system 100.

The third injection unit 420 is connected with the first interface of the third three-way valve 410, the first DIFF reagent tank 20 is connected with the second interface of the third three-way valve 410, the DIFF reaction detection system 100 is connected with the third interface of the third three-way valve 410, and the third three-way valve 410 can also be an electromagnetic valve to realize automatic switching of a communicating object, so that the first interface can be selectively communicated with the second interface and the third interface, thereby realizing that the first DIFF reagent is sucked from the first DIFF reagent tank 20 and discharged into the DIFF reaction detection system 100, and the first DIFF reagent can be accurately sucked through the cooperation of the third injection unit 420 and the third three-way valve 410, so that the accuracy of a result is ensured.

Optionally, with continued reference to fig. 1, a fourth three-way valve 510 and a fourth injection unit 520 are disposed on the second DIFF reagent line 500, one port on the fourth three-way valve 510 is used for communicating with the second DIFF reagent tank 30, and the other two ports on the fourth three-way valve 510 are respectively communicated with the fourth injection unit 520 and the DIFF reaction detection system 100, and the fourth injection unit 520 is used for introducing the second DIFF reagent in the second DIFF reagent tank 30 into the DIFF reaction detection system 100.

The fourth injection unit 520 is connected with the first interface of the fourth three-way valve 510, the second DIFF reagent tank 30 is connected with the second interface of the fourth three-way valve 510, the DIFF reaction detection system 100 is connected with the third interface of the fourth three-way valve 510, and the fourth three-way valve 510 can also be an electromagnetic valve to realize automatic switching of a communicating object, so that the first interface can be selectively communicated with the second interface and the third interface, thereby realizing that the second DIFF reagent is sucked from the second DIFF reagent tank 30 and discharged into the DIFF reaction detection system 100, and the second DIFF reagent can be accurately sucked through the cooperation of the fourth injection unit 520 and the fourth three-way valve 510, so that the accuracy of a result is ensured.

It should be understood that the third injection unit 420 and the fourth injection unit 520 may be electrically powered, and the motor is matched with the ball screw to change the rotation motion into the linear motion, so as to facilitate precisely controlling the strokes of the pistons in the third injection unit 420 and the fourth injection unit 520, thereby precisely controlling the sucking amounts of the first DIFF reagent and the second DIFF reagent.

Specifically, in one embodiment of the present application, the DIFF reaction detection system 100 includes a DIFF reaction detection unit 110, a fifth injection unit 120, and a sixth injection unit 130, wherein the DIFF reaction detection unit 110 includes a reaction region (not shown) and a detection region (not shown), the fifth injection unit 120 is in communication with the reaction region, the sixth injection unit 130 is in communication with the detection region, the fifth injection unit 120 is for providing power to push a sample on the reaction region into the detection region, and the sixth injection unit 130 is for providing sheath fluid into the detection region.

In the same case, the DIFF reaction detection unit 110 has a laser light source, a photo receiver, and a flow cell, and the laser light source, the photo receiver, and the flow cell are all located in the detection area.

In the reaction zone, the sample from the sample collection system 300 reacts and then enters the detection zone under the pushing action of the fifth injection unit 120, and a sheath flow is provided through the sixth injection unit 130, wherein the sheath flow is used for wrapping the sample flow and queuing the sample flow in the flow chamber to pass through the detection zone, when the sample flow passes through the detection zone after being wrapped by the sheath flow, the laser light source irradiates the sample, the photoelectric receiver detects scattered light or fluorescent signals, and the sample is wrapped in the sheath flow and queuing the sample flow in the flow chamber to pass through the flow chamber, so as to complete the measurement of the DIFF channel (leukocyte classification detection).

The fifth injection unit 120 may be a one-to-two syringe, and the reacted sample is sucked by one syringe, and the reacted sample is discharged by one syringe, and the operation of one syringe may be controlled by an electromagnetic valve, and when the reacted sample is discharged, power is provided to enable the sample to rapidly move from the reaction area to the detection area.

By the cooperation of the fifth injection unit 120 and the sixth injection unit 130, the sheath flow can wrap the sample flow at a high flow rate, and the sample flow is compressed to the detection spot diameter (5-10 μm) by the Bernoulli effect, so that cells can pass through the detection area one by one, and overlapping interference is avoided.

The fifth injection unit 120 and the sixth injection unit 130 may also adopt a manner of matching the power unit with the piston, the power unit may include a motor and a transmission member, the motor may adopt a stepping motor and a servo motor, and the transmission member may adopt a ball screw manner, so that the piston strokes in the fifth injection unit 120 and the sixth injection unit 130 may be accurately controlled.

In the description of the present application, it should be understood that, if there is an orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is merely for convenience of describing the present application and simplifying the description, and it is not intended to indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus the terms describing the positional relationship in the drawings are merely for exemplary illustration and are not to be construed as limitations of the present patent, and that the specific meaning of the terms described above may be understood by those skilled in the art according to specific circumstances.

The foregoing description of the preferred embodiments of the application is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the application.

Claims

1. A device for detecting five-class blood cells and MxA to identify a viral infection for diagnosing a viral infection and the extent thereof in an individual, comprising:

DIFF reaction detection system;

2. The device for detecting five-class blood cells and MxA for identifying a viral infection according to claim 1, wherein the MxA reagent card strip stacking unit comprises:

3. The device for detecting five-class blood cells and MxA for identifying a viral infection according to claim 2, wherein the MxA reagent card strip stacking unit comprises:

a mounting plate;

4. The device for detecting penta-classified blood cells and MxA to identify a viral infection of claim 1, further comprising:

5. The device for detecting penta-classified blood cells and MxA for the identification of viral infection of claim 4, further comprising a waste collection system, the waste liquid collection unit is in communication with the WBC pool, the RBC pool, the negative pressure chamber, and the DIFF reaction detection system, respectively.

6. The device for detecting penta-classified blood cells and MxA for identifying a viral infection according to claim 4, further comprising a diluent delivery line having a dispensing three-way valve thereon having a dispensing inlet for communicating with a diluent reservoir and two dispensing outlets in communication with the WBC reservoir and the RBC reservoir, respectively.

7. The device for detecting five-class blood cells and MxA for identifying a viral infection according to claim 6, wherein a first three-way valve and a first injection unit are provided on the diluent delivery tube, one port on the first three-way valve is used for communicating with a diluent reservoir, the other two ports on the first three-way valve are respectively communicated with the first injection unit and the dispensing inlet, and the first injection unit is used for introducing the diluent in the diluent reservoir into the dispensing three-way valve.

8. The device of claim 1, wherein the sample collection system comprises a second three-way valve, a second injection unit, and a sample collection unit, wherein one port on the second three-way valve is configured to communicate with a dilution reservoir, and wherein two other ports on the second three-way valve are configured to communicate with the second injection unit and the sample collection unit, respectively.

9. The device for detecting five-class blood cells and MxA for identifying a viral infection according to claim 1, wherein a third three-way valve and a third injection unit are provided on the first DIFF reagent line, one port on the third three-way valve is used to communicate with the first DIFF reagent pool, the other two ports on the third three-way valve are respectively communicated with the third injection unit and the DIFF reaction detection system, and the third injection unit is used to introduce the first DIFF reagent in the first DIFF reagent pool into the DIFF reaction detection system;

10. The device of claim 1, wherein the DIFF response detection system comprises a DIFF response detection unit comprising a reaction zone and a detection zone, a fifth injection unit in communication with the reaction zone, and a sixth injection unit in communication with the detection zone, the fifth injection unit for providing motive force to push a sample on the reaction zone into the detection zone, the sixth injection unit for providing sheath fluid into the detection zone.