CN121263530A - 用于通过进料分批转录制备加帽rna的方法和装置 - Google Patents

用于通过进料分批转录制备加帽rna的方法和装置

Info

Publication number
CN121263530A
CN121263530A CN202480028773.2A CN202480028773A CN121263530A CN 121263530 A CN121263530 A CN 121263530A CN 202480028773 A CN202480028773 A CN 202480028773A CN 121263530 A CN121263530 A CN 121263530A
Authority
CN
China
Prior art keywords
reaction
rna
feed
competing
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202480028773.2A
Other languages
English (en)
Chinese (zh)
Inventor
A·范·阿什贝克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ribopro BV
Original Assignee
Ribopro BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribopro BV filed Critical Ribopro BV
Publication of CN121263530A publication Critical patent/CN121263530A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202480028773.2A 2023-02-28 2024-02-27 用于通过进料分批转录制备加帽rna的方法和装置 Pending CN121263530A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2302887.1 2023-02-28
GBGB2302887.1A GB202302887D0 (en) 2023-02-28 2023-02-28 Rna
PCT/EP2024/054929 WO2024180048A2 (fr) 2023-02-28 2024-02-27 Arn

Publications (1)

Publication Number Publication Date
CN121263530A true CN121263530A (zh) 2026-01-02

Family

ID=85794061

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202480028773.2A Pending CN121263530A (zh) 2023-02-28 2024-02-27 用于通过进料分批转录制备加帽rna的方法和装置

Country Status (9)

Country Link
EP (1) EP4673553A2 (fr)
JP (1) JP2026506804A (fr)
KR (1) KR20250161571A (fr)
CN (1) CN121263530A (fr)
AU (1) AU2024229166A1 (fr)
GB (1) GB202302887D0 (fr)
IL (1) IL322948A (fr)
MX (1) MX2025010001A (fr)
WO (1) WO2024180048A2 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7074596B2 (en) 2002-03-25 2006-07-11 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Synthesis and use of anti-reverse mRNA cap analogues
US20050287539A1 (en) * 2004-06-29 2005-12-29 Emmanuel Labourier Methods and compositions for preparing capped RNA
CN113874502A (zh) 2019-03-11 2021-12-31 摩登纳特斯有限公司 补料分批体外转录方法
CA3173131A1 (fr) * 2020-04-16 2021-10-21 Michael Humbert Fabrication et purification in vitro d'arnm therapeutique

Also Published As

Publication number Publication date
IL322948A (en) 2025-10-01
WO2024180048A3 (fr) 2025-02-27
GB202302887D0 (en) 2023-04-12
EP4673553A2 (fr) 2026-01-07
WO2024180048A2 (fr) 2024-09-06
KR20250161571A (ko) 2025-11-17
JP2026506804A (ja) 2026-02-26
AU2024229166A1 (en) 2025-10-09
MX2025010001A (es) 2025-11-03

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