CN1331748A - 重组方法及其所用试剂 - Google Patents

重组方法及其所用试剂 Download PDF

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Publication number
CN1331748A
CN1331748A CN99814800A CN99814800A CN1331748A CN 1331748 A CN1331748 A CN 1331748A CN 99814800 A CN99814800 A CN 99814800A CN 99814800 A CN99814800 A CN 99814800A CN 1331748 A CN1331748 A CN 1331748A
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CN
China
Prior art keywords
recbcd
nucleotide sequence
homologous recombination
host cell
nucleotide sequences
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Pending
Application number
CN99814800A
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English (en)
Chinese (zh)
Inventor
P·艾欧安诺
K·纳拉亚南
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MURDOCH INSTITUTE
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MURDOCH INSTITUTE
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Publication of CN1331748A publication Critical patent/CN1331748A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN99814800A 1998-10-30 1999-09-29 重组方法及其所用试剂 Pending CN1331748A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPP6849 1998-10-30
AUPP6849A AUPP684998A0 (en) 1998-10-30 1998-10-30 A method of recombination and agents useful for same

Publications (1)

Publication Number Publication Date
CN1331748A true CN1331748A (zh) 2002-01-16

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ID=3811063

Family Applications (1)

Application Number Title Priority Date Filing Date
CN99814800A Pending CN1331748A (zh) 1998-10-30 1999-09-29 重组方法及其所用试剂

Country Status (5)

Country Link
EP (1) EP1127152A4 (fr)
JP (1) JP2002528129A (fr)
CN (1) CN1331748A (fr)
AU (1) AUPP684998A0 (fr)
WO (1) WO2000026396A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103068995A (zh) * 2010-06-10 2013-04-24 基因桥有限责任公司 直接克隆
CN103160495A (zh) * 2011-12-15 2013-06-19 张力军 一种非连接依赖性的快速克隆方法
WO2013143438A1 (fr) * 2012-03-30 2013-10-03 深圳市中联生物科技开发有限公司 Procédé de clonage moléculaire d'acide nucléique basé sur une recombinaison homologue et kit de réactifs associé
CN105087517A (zh) * 2015-08-18 2015-11-25 翌圣生物科技（上海）有限公司 一种重组酶复合物及体外同源重组无缝克隆的方法
CN106573038A (zh) * 2014-06-30 2017-04-19 默多克儿童研究所 螺杆菌治疗剂

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7517688B2 (en) 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
EP1178111A1 (fr) * 2000-08-03 2002-02-06 Lohmann Animal Health GmbH & Co. KG Vaccination contre des virus herpes associés aux cellules-hôtes
US7781190B2 (en) * 2003-07-24 2010-08-24 The University Of Hong Kong Method for constructing and modifying large DNA molecules

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69841340D1 (de) * 1997-12-05 2010-01-07 Europ Lab Molekularbiolog Neue Methode zur Klonierung DNS unter Anwendung des E. coli RecE/RecT Rekombinationssystems

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103068995A (zh) * 2010-06-10 2013-04-24 基因桥有限责任公司 直接克隆
CN103068995B (zh) * 2010-06-10 2020-10-16 基因桥有限责任公司 直接克隆
CN103160495A (zh) * 2011-12-15 2013-06-19 张力军 一种非连接依赖性的快速克隆方法
WO2013143438A1 (fr) * 2012-03-30 2013-10-03 深圳市中联生物科技开发有限公司 Procédé de clonage moléculaire d'acide nucléique basé sur une recombinaison homologue et kit de réactifs associé
CN106573038A (zh) * 2014-06-30 2017-04-19 默多克儿童研究所 螺杆菌治疗剂
CN105087517A (zh) * 2015-08-18 2015-11-25 翌圣生物科技（上海）有限公司 一种重组酶复合物及体外同源重组无缝克隆的方法

Also Published As

Publication number Publication date
EP1127152A1 (fr) 2001-08-29
JP2002528129A (ja) 2002-09-03
AUPP684998A0 (en) 1998-11-26
EP1127152A4 (fr) 2003-01-02
WO2000026396A1 (fr) 2000-05-11

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