EP1127152A4 - Methode de recombinaison et agents utiles dans ladite methode - Google Patents

Methode de recombinaison et agents utiles dans ladite methode

Info

Publication number
EP1127152A4
EP1127152A4 EP99950404A EP99950404A EP1127152A4 EP 1127152 A4 EP1127152 A4 EP 1127152A4 EP 99950404 A EP99950404 A EP 99950404A EP 99950404 A EP99950404 A EP 99950404A EP 1127152 A4 EP1127152 A4 EP 1127152A4
Authority
EP
European Patent Office
Prior art keywords
agents useful
recombination
recombination method
agents
useful
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99950404A
Other languages
German (de)
English (en)
Other versions
EP1127152A1 (fr
Inventor
Panos Ioannou
Kumaran Narayanan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MURDOCH INSTITUTE
MURDOCH INST
Original Assignee
MURDOCH INSTITUTE
MURDOCH INST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MURDOCH INSTITUTE, MURDOCH INST filed Critical MURDOCH INSTITUTE
Publication of EP1127152A1 publication Critical patent/EP1127152A1/fr
Publication of EP1127152A4 publication Critical patent/EP1127152A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
EP99950404A 1998-10-30 1999-09-29 Methode de recombinaison et agents utiles dans ladite methode Withdrawn EP1127152A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPP684998 1998-10-30
AUPP6849A AUPP684998A0 (en) 1998-10-30 1998-10-30 A method of recombination and agents useful for same
PCT/AU1999/000835 WO2000026396A1 (fr) 1998-10-30 1999-09-29 Methode de recombinaison et agents utiles dans ladite methode

Publications (2)

Publication Number Publication Date
EP1127152A1 EP1127152A1 (fr) 2001-08-29
EP1127152A4 true EP1127152A4 (fr) 2003-01-02

Family

ID=3811063

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99950404A Withdrawn EP1127152A4 (fr) 1998-10-30 1999-09-29 Methode de recombinaison et agents utiles dans ladite methode

Country Status (5)

Country Link
EP (1) EP1127152A4 (fr)
JP (1) JP2002528129A (fr)
CN (1) CN1331748A (fr)
AU (1) AUPP684998A0 (fr)
WO (1) WO2000026396A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7517688B2 (en) 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
EP1178111A1 (fr) * 2000-08-03 2002-02-06 Lohmann Animal Health GmbH & Co. KG Vaccination contre des virus herpes associés aux cellules-hôtes
US7781190B2 (en) * 2003-07-24 2010-08-24 The University Of Hong Kong Method for constructing and modifying large DNA molecules
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
CN103160495A (zh) * 2011-12-15 2013-06-19 张力军 一种非连接依赖性的快速克隆方法
CN102634534A (zh) * 2012-03-30 2012-08-15 深圳市中联生物科技开发有限公司 基于同源重组的核酸分子克隆方法及相关试剂盒
WO2016000022A1 (fr) * 2014-06-30 2016-01-07 Murdoch Childrens Research Institute Agent thérapeutique helicobacter
CN105087517B (zh) * 2015-08-18 2019-05-07 翌圣生物科技(上海)有限公司 一种重组酶复合物及体外同源重组无缝克隆的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509156B1 (en) * 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
ANDERSON D G ET AL: "SSB PROTEIN CONTROLS RECBCD ENZYME NUCLEASE ACTIVITY DURING UNWINDING: A NEW ROLE FOR LOOPED INTERMEDIATES", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 282, no. 2, 18 September 1998 (1998-09-18), pages 275 - 285, XP001057243, ISSN: 0022-2836 *
BOUDSOCQ FRANCOIS ET AL: "Quantitation of the inhibition of Hfr x F- recombination by the mutagenesis complex UmuD'C.", JOURNAL OF MOLECULAR BIOLOGY, vol. 270, no. 2, 1997, pages 201 - 211, XP002218282, ISSN: 0022-2836 *
HALL S D ET AL: "HOMOLOGOUS PAIRING AND STRAND EXCHANGE PROMOTED BY THE ESCHERICHIA COLI RECT PROTEIN", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, 1 April 1994 (1994-04-01), pages 3205 - 3209, XP002064299, ISSN: 0027-8424 *
HALL S D ET AL: "IDENTIFICATION AND CHARACTERIZATION OF THE ESCHERICHIA COLI RECT PROTEIN, A PROTEIN ENCODED BY THE RECE REGION THAT PROMOTES RENATURATION OF HOMOLOGOUS SINGLE-STRANDED DNA", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 175, no. 1, 1993, pages 277 - 287, XP002064298, ISSN: 0021-9193 *
KOWALCZYKOWSKI S C ET AL: "BIOCHEMISTRY OF HOMOLOGOUS RECOMBINATION IN ESCHERICHIA COLI", MICROBIOLOGICAL REVIEWS, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, US, vol. 58, no. 3, September 1994 (1994-09-01), pages 401 - 465, XP001093135, ISSN: 0146-0749 *
MURPHY ET AL: "Use of bacteriophage lambda recombination funtions to promote gene replacement in Escherichia coli", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 180, no. 8, April 1998 (1998-04-01), pages 2063 - 2071, XP002149589, ISSN: 0021-9193 *
MURPHY KENAN C: "Biochemical characterization of P22 phage-modified Escherichia coli RecBCD enzyme.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 36, 1994, pages 22507 - 22516, XP002218281, ISSN: 0021-9258 *
NARAYANAN K ET AL: "EFFICIENT AND PRECISE ENGINEERING OF A 200 KG BETA-GLOBIN HUMAN/BACTERIAL ARTIFICIAL CHROMOSOME IN E.COLI DH10B USING AN INDUCIBLE HOMOLOGOUS RECOMBINATION SYSTEM", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 6, no. 3, March 1999 (1999-03-01), pages 442 - 447, XP000946877, ISSN: 0969-7128 *
NARAYANAN KUMARAN ET AL: "GET recombination, a novel E. coli inducible homologous recombination system for BACs.", EUROPEAN JOURNAL OF HUMAN GENETICS, vol. 7, no. SUPPL. 1, July 1999 (1999-07-01), 31st Annual Meeting of the European Society of Human Genetics;Geneva, Switzerland; May 29-June 1, 1999, pages 109, XP001118691, ISSN: 1018-4813 *
POTEETE A R ET AL: "ACTIVATION OF REC-F-DEPENDENT RECOMBINATION IN ESCHERICHIA-COLI BY BACTERIOPHAGE LAMBDA AND P22-ENCODED FUNCTIONS", JOURNAL OF BACTERIOLOGY, vol. 170, no. 9, 1988, pages 4379 - 4381, XP001118714, ISSN: 0021-9193 *
POTEETE A R ET AL: "LAMBDA RED DEPENDENT GROWTH AND RECOMBINATION OF PHAGE P-22", VIROLOGY, vol. 134, no. 1, 1984, pages 161 - 167, XP001118765, ISSN: 0042-6822 *
POTEETE A R ET AL: "MODULATION OF ESCHERICHIA-COLI REC-BCD ACTIVITY BY THE BACTERIOPHAGE LAMBDA GAM AND P22 ABC FUNCTIONS", JOURNAL OF BACTERIOLOGY, vol. 170, no. 5, 1988, pages 2012 - 2021, XP001117996, ISSN: 0021-9193 *
POTEETE ANTHONY R ET AL: "Efficient double-strand break-stimulated recombination promoted by the general recombination systems of phages lambda and P22.", GENETICS, vol. 134, no. 4, 1993, pages 1013 - 1021, XP001118686, ISSN: 0016-6731 *
See also references of WO0026396A1 *

Also Published As

Publication number Publication date
AUPP684998A0 (en) 1998-11-26
JP2002528129A (ja) 2002-09-03
CN1331748A (zh) 2002-01-16
EP1127152A1 (fr) 2001-08-29
WO2000026396A1 (fr) 2000-05-11

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