CN1631902A - A kind of angelica polysaccharide and its preparation method and application - Google Patents

Abstract

The invention relates to a Chinese angelica polysaccharide, which is made in the following way: dissolve alcohol deposit of Chinese angelica into distilled water, use savage method to more the protein away until there is no protein absorption peak of 280 nm by ultraviolet test, centrifugation to clear the deposit; keep the water-phase and add into alcohol for depositing polysaccharide, abandon the supernatant liquid, wash the deposit by alcohol, then freeze and dry it, through gel molecular chromatography, accumulate the separated product with the relative molecule mass between 10,000-110,000, then freeze and dry it again to acquire the Chinese angelica polysaccharide. The invention has remarkable analgesia for ache caused by chemicals and hot simulation; as well as dysmenorrheal caused by female hormone; and it can be made into drug for analgesia especially that for curing dysmenorrheal.

Description

A kind of angelica polysaccharide and its preparation method and application

technical field

The invention relates to an angelica polysaccharide, a preparation method and application thereof.

Background technique

Angelica belongs to tonic medicine, which is the dry root of Angelica sinensis (Oliv) Diels, a perennial herb belonging to the umbrella family (umbuiferae). Angelica has the effects of enriching blood and blood, promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain. Clinically, angelica is often used in combination with other medicines for irregular menstruation, dysmenorrhea, blood deficiency headache, waist and abdomen pain and other diseases.

The chemical composition of angelica includes two major parts of volatile oil and water-soluble components. Early studies have pointed out that angelica oil has a relaxing effect on isolated uteri of various animals, both pregnant and non-pregnant. It partly explains the use of Angelica sinensis in the treatment of dysmenorrhea.

Angelica polysaccharide exists in the water-soluble part of Angelica sinensis, which is a homologous mixture composed of polymer compounds with different molecular weights. In recent years, Angelica polysaccharides have been proved to have complex biological activities, which can regulate the body's immune function, resist the inhibition caused by corticosteroids, prevent the reduction of peripheral leukocytes and the post-irradiation effect in radiation damage, enhance the body's hematopoietic function and anti-tumor effect.

Pain is a common clinical symptom, and finding safe and effective non-addictive analgesics has always been a problem that drug workers attach great importance to. "Dysmenorrhea" is a common disease in gynecology. Traditional Chinese medicine believes that its pathogenesis is mainly due to obstruction of Qi and blood and poor blood circulation. Angelica is an important gynecological medicine in traditional Chinese medicine. How to find its effective therapeutic ingredients to modernize traditional Chinese medicine and make it meet the requirements of modern dosage forms is a research hotspot in the field of pharmacy.

"Chinese Journal of Pharmaceutical Sciences" 2002; 37(10): 746-8 "Experimental Study on the Analgesic Effect of Angelica Crude Polysaccharides" revealed that Angelica sinensis crude polysaccharides have analgesic effects, but the sugar content in the Angelica sinensis crude polysaccharides used is relatively low and contains other ingredients, and the analgesic effect is not obvious.

Contents of the invention

The problem to be solved by the present invention is to provide a preparation method and application of angelica polysaccharide, the obtained angelica polysaccharide has obvious analgesic effect, especially for treating "dysmenorrhea".

The technical scheme provided by the invention is: a kind of angelica polysaccharide, which is prepared by the following method: dissolve the alcohol precipitate of angelica sinensis in distilled water, use the Sevag method to remove protein until there is no protein absorption peak at 280nm in ultraviolet detection, and remove the precipitate by centrifugation; keep the water phase, Add absolute ethanol to precipitate the polysaccharide, discard the supernatant, wash the obtained precipitate with absolute ethanol, freeze-dry, and separate by gel molecular exclusion chromatography. The relative molecular mass range of the collection is 1,0000-11,0000 The isolated product between them was freeze-dried to obtain Angelica polysaccharide.

The present invention also provides a preparation method of the above-mentioned angelica polysaccharide, which comprises dissolving the alcohol precipitate of angelica sinensis in distilled water, removing the protein by Sevag method until there is no protein absorption peak at 280nm in ultraviolet detection, and centrifuging to remove the precipitate; retaining the water phase, adding absolute ethanol, and precipitating Polysaccharide, discard the supernatant, wash the obtained precipitate with absolute ethanol until the washing liquid is colorless, discard the washing liquid, freeze-dry the washed precipitate, separate by gel size exclusion chromatography, and collect the relative molecular mass range The isolated product between 1,0000-110,000 is freeze-dried to obtain Angelica polysaccharide.

The alcohol precipitate of Angelica sinensis raw material in the present invention can come from the alcohol precipitate of Angelica sinensis obtained by the common extraction method of Chinese medicinal materials in the preparation process of traditional Chinese medicine: that is, the water extract of Angelica sinensis is boiled in water, and the precipitate of the water extract is extracted with ethanol.

The above-mentioned angelica polysaccharide obtained in the present invention belongs to a polymer, and its molecular weight represents the average distribution of similar chain lengths. The physical and chemical properties of the angelica polysaccharide identified in the present invention are as follows: the relative molecular mass range is between 1,0000-11,0000 (using gel permeation chromatography-laser light scattering combined technology), easily soluble in water, and the sugar content is greater than 95 %, HPLC detection shows that it is mainly composed of glucose, arabinose, galactose, rhamnose and xylose, and the ultraviolet absorption spectrum shows an obvious absorption peak at 192nm; infrared absorption spectrum analysis, at 3352cm ^-1 , 2940cm ^-1 , Typical polysaccharide absorption peaks were observed at 1747cm ^-1 , 1626cm ^-1 , 1414cm ^-1 , 1237cm ^-1 , 1021cm ^-1 and 536cm ^-1 .

Use of the above-mentioned angelica polysaccharide of the present invention in the preparation of analgesic drugs, especially drugs for treating dysmenorrhea. If the angelica polysaccharide of the present invention is used to prepare capsules, tablets, granules and injections, the obtained medicine has obvious analgesic effect, especially the effect of treating "dysmenorrhea" is remarkable.

When the Angelica sinensis crude polysaccharide (sugar content is 65.5%) in the background technology is once-administered 0.25g/kg, it has obvious analgesic effect to the model that chemical substance and heat stimulation cause pain, and the Angelica sinensis polysaccharide of the present invention once A comparable effect can be produced when oral administration of 0.025g/kg is administered, the dosage is reduced by 90%, and the analgesic effect is enhanced with the increase of dosage.

Detailed ways

The technical scheme of the present invention will be further described below in conjunction with specific examples:

The alcohol precipitate of Angelica sinensis raw material in the present invention can come from the alcohol precipitate of Angelica sinensis obtained by the common extraction method of Chinese medicinal materials in the preparation process of traditional Chinese medicine: that is, the water extract of Angelica sinensis is boiled in water, and the precipitate of the water extract is extracted with ethanol.

Angelica alcohol precipitation can also be obtained by the following method: the angelica alcohol precipitation is prepared according to the following method, take angelica decoction pieces, add water 5 to 7 times the weight of angelica decoction pieces, decoct for 30 to 60 minutes, filter; Decoct 3-6 times the weight (dregs) of water for 30-50 minutes, filter; add 3-6 times the weight (dregs) of water to decoct for 30-50 minutes, filter; combine the three filtrates , and concentrate the filtrate; combine the three filtrates, concentrate, cool to room temperature, add ethanol until the ethanol content is 40% to 60% (volume ratio, the same below), refrigerate at 2 to 4°C for 24 to 48 hours, filter to obtain a precipitate Concentrate again, add ethanol until the ethanol content is 70%-80%, refrigerate at 2-4°C for 24-48 hours, filter to obtain a precipitate; concentrate for the third time, add ethanol until the ethanol content is 80%-90% , refrigerated at 2 to 4°C for 24 to 48 hours, filtered to obtain a precipitate; the three precipitates were combined to obtain an angelica alcohol precipitate.

The preparation of angelica polysaccharides of the present invention: dissolve the alcohol precipitate of angelica sinensis in distilled water, add ethanol until the ethanol content is 70% to 80% for extraction, refrigerate at 2 to 4°C for 24 to 48 hours, discard the supernatant, keep the precipitate, and repeat the process Step until the supernatant is colorless, save the pellet. The precipitate was redissolved in distilled water again, protein was removed by Sevag method until there was no protein absorption peak at 280nm in ultraviolet detection, and denatured protein was removed by centrifugation. Keep the water phase, add 3 to 4 times the volume of absolute ethanol, refrigerate at 2 to 4°C for 24 to 48 hours, and discard the supernatant. The precipitated part is washed with absolute ethanol until the washing liquid is colorless, discarding the washing liquid, and after the ethanol volatilizes naturally, freeze-dry for 5-20 hours. The obtained product belongs to a polymer, and its molecular weight represents the average distribution of similar chain lengths. Separation by gel molecular exclusion chromatography, collecting isolated products with a relative molecular mass ranging from 1,0000 to 11,0000, and then freeze-drying to obtain Angelica polysaccharide. The physical and chemical properties of Angelica polysaccharide obtained in the present invention are identified as follows: the relative molecular mass range is between 1,0000-11,0000 (using gel permeation chromatography-laser light scattering combined technology), easily soluble in water, and the sugar content is greater than 95 %, HPLC detection shows that it is mainly composed of glucose, arabinose, galactose, rhamnose and xylose, and the ultraviolet absorption spectrum shows an obvious absorption peak at 192nm; infrared absorption spectrum analysis, at 3352cm ^-1 , 2940cm ^-1 , Typical polysaccharide absorption peaks were observed at 1747cm ^-1 , 1626cm ^-1 , 1414cm ^-1 , 1237cm ^-1 , 1021cm ^-1 and 536cm ^-1 .

Embodiment 1: Take Angelica decoction pieces and add 5 times (by weight) water to decoct for 30 minutes, filter; add 3 times (weight ratio) water to the obtained medicinal residues and decoct for 30 minutes, filter; add 3 times the obtained medicinal residues (weight ratio) decoct in water for 30 minutes, filter; combine the three filtrates, and concentrate the filtrate. Combine the three filtrates, concentrate, cool to room temperature, add ethanol until the ethanol content is 40% (v/v), refrigerate at 4°C for 24 hours, filter, keep the precipitate that is the alcohol precipitate of Angelica sinensis; concentrate again, add ethanol until the ethanol content is 70% (v/v), refrigerated at 4°C for 24 hours, filtered, and retained the precipitate, namely the alcohol precipitate of Angelica sinensis; the third concentration, adding ethanol until the ethanol content was 80% (v/v), refrigerated at 4°C for 24 hours, Filtrate, and keep the precipitate, that is, the angelica alcohol precipitate. Merge the precipitation, that is, combine the alcohol precipitate of Angelica sinensis, redissolve it in distilled water, add ethanol until the ethanol content is 70% (v/v) for extraction, refrigerate at 4°C for 24 hours, discard the supernatant, keep the precipitate, and repeat this step until The supernatant was colorless and the precipitate remained. The precipitate was redissolved in distilled water again, the protein was removed by Sevag method until there was no protein absorption peak at 280nm by UV detection, and the denatured protein was removed by centrifugation. Keep the aqueous phase, add 4 times the volume of absolute ethanol, refrigerate at 4°C for 24 hours, and discard the supernatant. The precipitated part was washed with absolute ethanol until the washing solution was colorless, discarded the washing solution, and after the ethanol volatilized naturally, freeze-dried for 5 hours; with a TSK-Gel4000H column (pore diameter of 600 angstroms), the column size was 250 × 10mm, Using distilled water as the mobile phase with a flow rate of 10ml/min, collecting the isolated product for 5-60min; and then freeze-drying to obtain Angelica polysaccharide. The gel permeation chromatography-laser light scattering combined technique, HPLC and infrared absorption spectrum analysis were used to identify the yield of the polysaccharide of Angelica sinensis 10%, and the purity was 96%.

Embodiment 2: Take Angelica decoction pieces and add 7 times (weight ratio) water to decoct for 60 minutes, filter; add 5 times (weight ratio) water to the obtained medicinal residues and decoct for 40 minutes, filter; obtained medicinal residues add 3 times (weight ratio) decoct in water for 40 minutes, filter; combine the three filtrates, and concentrate the filtrate. Combine the three filtrates, concentrate, cool to room temperature, add ethanol until the ethanol content is 60% (v/v), refrigerate at 2°C for 48 hours, filter, keep the precipitate that is the alcohol precipitate of Angelica sinensis; concentrate again, add ethanol until the ethanol content is 80% (v/v), refrigerated at 2°C for 48 hours, filtered, and retained the precipitate that is the alcohol precipitate of Angelica sinensis; the third concentration, adding ethanol until the ethanol content was 80% (v/v), refrigerated at 2°C for 48 hours, Filtrate, and keep the precipitate, that is, the angelica alcohol precipitate. Merge the precipitation, that is, combine the alcohol precipitate of Angelica sinensis, redissolve it in distilled water, add ethanol until the ethanol content is 80% (v/v) for extraction, refrigerate at 2°C for 48 hours, discard the supernatant, keep the precipitate, and repeat this step until The supernatant was colorless and the precipitate remained. The precipitate was redissolved in distilled water again, protein was removed by Sevag method until there was no protein absorption peak at 280nm in ultraviolet detection, and denatured protein was removed by centrifugation. Keep the aqueous phase, add 3 times the volume of absolute ethanol, refrigerate at 2°C for 48 hours, and discard the supernatant. The precipitated part was washed with absolute ethanol until the washing liquid was colorless, and the washing liquid was discarded. After the ethanol volatilized naturally, it was freeze-dried for 10 hours; with a TSK-Gel4000H column (pore diameter 600 angstroms), the column size was 250 × 10mm, Using distilled water as the mobile phase, the flow rate is 10ml/min, and the isolated product is collected for 5-60min; then freeze-dried to obtain the polysaccharide of Angelica sinensis. The gel permeation chromatography-laser light scattering combined technique, HPLC and infrared absorption spectrum analysis were used to identify the yield of the polysaccharide from Angelica sinensis to 30%, and the purity was 97%.

Embodiment 3: Get Angelica decoction pieces and add 6 times (weight ratio) water to decoct for 50 minutes, filter; Add 6 times (weight ratio) water to the obtained medicinal residues and decoct for 50 minutes, filter; Gained medicinal residues add 6 times (weight ratio) decoct in water for 50 minutes, filter; combine three filtrates, and concentrate the filtrate. Combine the three filtrates, concentrate, cool to room temperature, add ethanol until the ethanol content is 50% (v/v), refrigerate at 4°C for 48 hours, filter, keep the precipitate, which is the alcohol precipitate of Angelica sinensis; concentrate again, add ethanol until the ethanol content is 80% (v/v), refrigerated at 4°C for 48 hours, filtered, and retained the precipitate that is the alcohol precipitate of Angelica sinensis; the third concentration, adding ethanol until the ethanol content was 90% (v/v), refrigerated at 4°C for 48 hours, Filtrate, and keep the precipitate, that is, the angelica alcohol precipitate. Merge the precipitation, that is, combine the alcohol precipitate of Angelica sinensis, redissolve it in distilled water, add ethanol until the ethanol content is 70% (v/v) for extraction, refrigerate at 4°C for 48 hours, discard the supernatant, keep the precipitate, and repeat this step until The supernatant was colorless and the precipitate remained. The precipitate was redissolved in distilled water again, protein was removed by Sevag method until there was no protein absorption peak at 280nm in ultraviolet detection, and denatured protein was removed by centrifugation. Keep the aqueous phase, add 4 times the volume of absolute ethanol, refrigerate at 4°C for 24 hours, and discard the supernatant. The precipitated part was washed with absolute ethanol until the washing solution was colorless, and the washing solution was discarded. After the ethanol volatilized naturally, it was freeze-dried for 20 hours; with a TSK-Gel4000H column (pore diameter 600 angstroms), the column size was 250 × 10mm, Using distilled water as the mobile phase, the flow rate is 10ml/min, and the isolated product is collected for 5-60min; then freeze-dried to obtain the polysaccharide of Angelica sinensis. The gel permeation chromatography-laser light scattering combined technique, HPLC and infrared absorption spectrum analysis were used to identify the yield of the polysaccharide from Angelica sinensis to 40%, and the purity was 96%.

Example 4: Preparation of analgesic drug using Angelica polysaccharide of the present invention: Angelica polysaccharide is added with excipients, and made into tablets according to conventional methods.

Example 5: Preparation of analgesic drug using Angelica polysaccharide of the present invention: Angelica polysaccharide is added with excipients and made into capsules according to conventional methods.

Example 6: Preparation of analgesic drug using Angelica polysaccharide of the present invention: Angelica polysaccharide was dissolved in water for injection, and injection was prepared according to conventional methods.

Animal testing:

1. Drug: diethylstilbestrol injection, Shanghai Ninth Pharmaceutical Factory, batch number: 020906. Oxytocin injection, Nanjing Biochemical Pharmaceutical Factory, batch number: 030409.

(2) The main instrument GL-8402 hot plate pain measuring instrument, produced by Zhejiang Ninghai Medical Instrument Factory.

(3) Animal health Kunming mice, except for the "dysmenorrhea model" which uses ♂ mice as negative controls, other experiments use ♀ mice, weighing 18±1g, provided by the Animal Experiment Center of Wuhan University, certificate number: SCXK (E) 2003-2004.

2. Method

(1) Effect of Angelica polysaccharide of the present invention on writhing response of female mice caused by acetic acid

See Table 1 for animal grouping and drug dosage. Angelica sinensis crude polysaccharide (sugar content: 65.5%) in the background technology or Angelica sinensis polysaccharide of the present invention was orally administered 1 hour before the experiment, and the model group was given normal saline of equal volume. One hour after administration, 0.2ml of 1% acetic acid was injected intraperitoneally, and the latent period of writhing reaction and the number of times of writhing within 30 minutes were observed in the administered mice, and the protection percentage was calculated.

(2) The influence of Angelica polysaccharide of the present invention on the analgesic effect of hot plate method

The ♀ mice were screened on a 55°C hot plate in advance, and the mice that showed a foot-licking response within 10 to 30 seconds were qualified mice. The experimental groups and drug doses are shown in Table 2. One hour before the experiment, the test drug was given by oral gavage (ig), and the model group was given the same volume of normal saline. In order to avoid the influence of the periodic fluctuation of the pain threshold, the experiments were carried out at 8-10 a.m. at a room temperature of 25±1°C. The change of pain threshold in each group was recorded, and the percentage increase of pain threshold was calculated. If there is no foot licking reaction within 60 seconds, the pain threshold is counted as 60 seconds.

(3) Effect of Angelica polysaccharide of the present invention on diethylstilbestrol and oxytocin-induced mouse uterine spasms

Animals in each group were subcutaneously injected with 0.2 mg of diethylstilbestrol per animal for 3 consecutive days before the experiment. On the day of the experiment, the animals were orally administered with Angelica sinensis crude polysaccharide or an Angelica sinensis polysaccharide described in the present invention, and the model group was given an equal volume of normal saline, and ip oxytocin 0.3u/animal after 1 hour. The latent period of writhing reaction and the number of writhing times within 30 min were observed in each group. If there is no writhing reaction within 30 minutes, the incubation period is counted as 30 minutes.

3. Results

(1) The effect experiment of Angelica polysaccharide of the present invention on pain model—writhing response of female mice induced by acetic acid, the results are shown in Table 1.

Table 1 The influence of Angelica polysaccharide of the present invention on writhing reaction caused by acetic acid. n (number of cases)=10, x ± s (mean ± standard deviation)

writhing reaction

Group Dose (g·kg ^-1 )

Incubation period (min) Times (times/30min) Protection rate (%)

Model group / 5.2±1.8 34.2±8.8 /

Angelica crude polysaccharide 0.075 6.9±1.5 ¹⁾ 22.9±6.9 ²⁾ 33.0

0.250 19.3±6.8 ²⁾ 7.4±5.0 ²⁾ 78.4

An angelica polysaccharide 0.025 14.1±7.8 ²⁾ 10.3±5.3 ²⁾ 69.9

0.075 23.1±6.9 ²⁾ 5.9±4.8 ²⁾ 82.7

Note: Compared with the model control group, ¹⁾ P<0.05, ²⁾ P<0.01.

The results show that the angelica polysaccharide of the present invention can obviously produce a protective effect on the pain caused by acetic acid at only 1/10 dose of the angelica crude polysaccharide, and the effect is enhanced with the increase of the dose.

(2) Experiment of the influence of the polysaccharide of Angelica sinensis of the present invention on the pain-inducing effect of the hot plate method. The results are shown in Table 2.

Table 2 The effect of Angelica polysaccharide of the present invention on the pain induced by hot plate method in female mice. n (number of cases)=10, x ± s (mean ± standard deviation)

Pain threshold before administration

Dosage After administration Pain threshold Pain threshold increased Pain threshold increased

group room

(g·kg ^-1 ) Room(s) Room(s) Rate(%)

(s)

Model group / 20.8±6.4 21.2±6.8 1.3±1.5 1.9

Angelica crude polysaccharide 0.075 21.5±6.3 24.9±7.7 4.4±5.4 15.8

0.250 23.4±4.4 34.6±9.3 11.7±8.2 ¹⁾ 47.9

An angelica polysaccharide 0.025 20.7±5.2 29.4±9.1 9.1±8.1 ¹⁾ 42.0

0.075 21.3±6.2 34.8±8.7 13.7±9.6 ¹⁾ 63.4

Note: Compared with the control group, ¹⁾ P<0.01.

The results show that the angelica polysaccharide of the present invention can significantly protect the pain caused by heat stimulation at only 1/10 dose of the angelica crude polysaccharide, and the effect is enhanced with the increase of the dose.

(3) The experiment of the effect of Angelica polysaccharide of the present invention on the "dysmenorrhea model" - diethylstilbestrol and oxytocin-induced uterine cramps in mice, the results are shown in Table 3.

Table 3 Effect of Angelica polysaccharide of the present invention on uterine spasm in mice induced by diethylstilbestrol and oxytocin.

n (number of cases) = 10, x ± s (mean ± standard deviation)

writhing reaction

Group Dose (g·kg ^-1 )

Incubation period (min) Times (times/30min) Occurrence rate (%)

Model Group / / 12.3±3.8 6.3±4.0 100

Angelica crude polysaccharide 0.075 21.1±9.0 ¹⁾ 2.0±2.1 ¹⁾ 60

0.250 23.2±8.8 ²⁾ 1.3±1.9 ²⁾ 40 ¹⁾

An angelica polysaccharide 0.025 25.4±6.6 ²⁾ 0.9±1.3 ²⁾ 40 ¹⁾

0.075 27.7±4.9 ²⁾ 0.6±1.3 ²⁾ 30 ²⁾

Note: Compared with the model group, ¹⁾ P<0.05, ²⁾ P<0.01.

The above results show that the one-time oral administration of 0.025g/kg and 0.075g/kg of Angelica polysaccharide of the present invention has obvious analgesic effect on the pain model caused by chemical substances and heat stimulation, and significantly inhibits the pain caused by "dysmenorrhea" in mice. The body response can effectively counteract the pain response caused by estrogen-induced "dysmenorrhea". Experiments show that the angelica polysaccharide of the present invention has a significant effect at 1/10 dose of the angelica crude polysaccharide, and the effect is enhanced with the increase of the dose.

Claims (5)

1. Radix Angelicae Sinensis polysaccharide is made by laxative remedy: the angelol hypostasis is dissolved in distilled water, does not have the protein absorption peak of 280nm, the centrifugal precipitation of removing with Sevag method Deproteinization until ultraviolet detection; Keep water, add dehydrated alcohol, the precipitation polysaccharide is abandoned supernatant liquor, the gained throw out is with absolute ethanol washing, and after lyophilize, the gel molecular exclusion chromatography separates, and collects the relative molecular mass scope 1,0000-11, the separated product between 0000 obtains Radix Angelicae Sinensis polysaccharide again after lyophilize.

2. Radix Angelicae Sinensis polysaccharide according to claim 1 is characterized in that: described angelol hypostasis is pressed method and is made, and gets angelica sinensis, and the decocting that adds 5～7 times of angelica sinensis weight boiled 30～60 minutes, filters; The water that the gained dregs of a decoction is added 3～6 times of weight decocted 30～50 minutes, filtered; The decocting that the gained dregs of a decoction add 3～6 times of weight boiled 30～50 minutes, filtered; Merge three times filtrate, and filtrate is concentrated; Merge three times filtrate, concentrate, be cooled to room temperature, adding ethanol to ethanol content is 40%～60%, 2～4 ℃ of refrigerations 24～48 hours, filters, and gets throw out; Concentrate once more, adding ethanol to ethanol content is 70%～80%, 2～4 ℃ of refrigerations 24～48 hours, filters, and gets throw out; Concentrate for the third time, adding ethanol to ethanol content is 80%～90%, 2～4 ℃ of refrigerations 24～48 hours, filters, and gets throw out; Merge three throw outs and get Radix Angelicae Sinensis alcohol precipitation thing.

3. the preparation method of the described Radix Angelicae Sinensis polysaccharide of claim 1 is characterized in that: the angelol hypostasis is dissolved in distilled water, does not have the protein absorption peak of 280nm, the centrifugal precipitation of removing with Sevag method Deproteinization until ultraviolet detection; Keep water, add dehydrated alcohol, the precipitation polysaccharide is abandoned supernatant liquor, the gained throw out is colourless with absolute ethanol washing to washings, discard washings, the throw out after the washing is through lyophilize, and the gel molecular exclusion chromatography separates, collect the relative molecular mass scope 1,0000-11, the separated product between 0000 obtains Radix Angelicae Sinensis polysaccharide again after lyophilize.

4. claim 1 or 2 described Radix Angelicae Sinensis polysaccharides are in the purposes of preparation in the analgesic.

5. claim 1 or 2 described Radix Angelicae Sinensis polysaccharides are preparing the purposes for the treatment of in the dysmenorrhoea medicine.