CN209727806U - A sample stabilization device based on optical projection tomography - Google Patents

Abstract

The present application provides a sample stabilization device based on optical projection tomography, which includes a main frame, a fixing mechanism for fixing the sample is arranged on the main frame, a rotating device for rotating the sample is arranged on the fixing mechanism, the The main frame is also provided with a positioning device, the positioning device is arranged below the fixing mechanism, one end of the positioning device is connected with the main frame, and the positioning device includes a through hole for the sample to pass through. The positioning device also includes a stabilizer, and the stabilizer is arranged in cooperation with the through hole. On the one hand, the positioning device in this application plays a positioning role in fixing the position of the sample. On the other hand, a stabilizer is provided in the positioning device, so that the sample is more stable when it is moving or rotating, which is more conducive to the stability of imaging.

Description

A sample stabilization device based on optical projection tomography

technical field

The present application relates to the technical field of molecular imaging, in particular to a sample stabilization device based on optical projection tomography.

Background technique

Optical projection tomography (OPT) technology uses the characteristics of light to travel along a straight line in a small-sized organism, emits visible light to penetrate the sample, or uses a laser to excite the sample to generate fluorescence, and then uses a camera to collect projection views of the sample from multiple angles. Perform 3D imaging. Specifically, when performing optical projection tomography, the sample needs to be scanned from multiple angles. Generally, an electronically controlled turntable is used to rotate the sample step by step, and one or more projection images are collected for each rotation to an angle. The final data collected by the circular orbit scanning of the optical projection tomography system is a series of two-dimensional projection images of light passing through the sample at different angles, and three-dimensional images can be obtained through corresponding reconstruction algorithms. Through the above-mentioned technology, high-resolution, structural-functional integration, and radiation-free qualitative and quantitative research on small-scale living organisms at the cellular level can be achieved, and real-time, non-invasive, dynamic, and in-vivo imaging of organisms can be realized. In order to reduce the refraction and reflection effects of light on the surface of the biological body, the existing technology center places the capillary glass tube containing the sample in the matching liquid.

An optical projection tomography system is disclosed in CN102743159A, including: a light source module, used to provide white light or laser for illuminating the sample; a sample carrying positioning module, used to adjust the position of the sample and drive the sample to rotate to collect projections from multiple angles Data; the signal acquisition module is used to realize the convergence of optical signals and selectively collect optical signals; the central control module is used to coordinate the orderly operation of various parts, realize the opening and closing and intensity adjustment of the light source, and the center of the translation platform Calibration, parameter setting of the rotary table, parameter setting of the EMCCD camera, data processing and data storage; peripheral modules of the equipment are used to provide an anti-interference and stable environment for imaging. In this application, the capillary glass tube rotates relative to the matching liquid tank. If the capillary glass tube is not stabilized, the matching liquid will be agitated. In severe cases, there will be air bubbles, which may cause imaging effects during imaging.

Utility model content

In order to solve the above-mentioned technical problems, the present application proposes a sample stabilization device based on optical projection tomography, which includes a main frame, and a fixing mechanism for fixing the sample is arranged on the main frame, and a fixing mechanism for sample rotation is arranged on the fixing mechanism. The main frame is also provided with a positioning device, the positioning device is arranged below the fixing mechanism, one end of the positioning device is connected with the main frame, and the positioning device includes a sample for passing through The positioning device also includes a stabilizer, and the stabilizer is arranged in cooperation with the through hole. The fixing mechanism in this application is used to fix the sample in this application. The rotating device in the fixing mechanism is used for the rotation of the sample. On the one hand, the positioning device plays a role in positioning the position of the sample. Components make the sample more stable when moving or rotating, which is more conducive to the stability of imaging.

Further, the main frame includes a base and a support frame, the support frame is connected to the base, and the support frame is vertically arranged above the base; the fixing mechanism and the positioning device are both arranged on the support on the shelf. The main frame of the present application is provided with a support frame on the base to make the whole structure more stable, and the fixing mechanism and positioning device are arranged on the support frame to make the whole device more convenient to use.

Further, the stabilizer includes several elastic pieces, one end of which is connected to the inner wall of the through hole, the several elastic pieces are evenly arranged in the through hole, and the other ends of the several elastic pieces are matched to form the inner wall of the through hole. channel through which the sample passes. On the one hand, the setting of the elastic member makes the rotation of the sample more stable after entering the channel, and there will be no shaking and other conditions that affect the imaging effect; on the other hand, the device is suitable for samples of different sizes.

Further, several cleaning parts for cleaning the outside of the sample are provided inside the channel. The surface of the sample is wiped by the cleaning part before imaging, which is more conducive to the clarity of the image; on the other hand, if the sample is placed in the matching liquid tank, after the test is completed, the surface of the sample is covered with matching liquid. If it is not wiped off in time , may affect the operation of electronic components.

Further, the base is provided with a clamping and fixing part for placing a matching liquid tank, the clamping and fixing part is arranged under the positioning device, and the clamping and fixing part is arranged in cooperation with the channel. The installation and fixation of the matching liquid tank is also very important for the stability of the imaging. In this application, the clamping and fixing part is used to install the matching liquid tank at a corresponding position.

Further, the clamping and fixing part includes several clamping grooves cooperating with the matching liquid tank, and one end of the clamping grooves is fixedly connected with the base.

Further, several locking protrusions are arranged inside the locking groove. The frictional force between the snap-in groove and the matching liquid tank is increased by using the snap-in protrusion.

Further, the main frame is provided with a lifting device for the lifting movement of the fixing mechanism, and the lifting device includes a first slideway arranged on the support frame, and the fixing mechanism is arranged in cooperation with the first slideway the first sliding end of . In this application, the lifting device is used to drive the fixing mechanism to move up and down, thereby driving the sample to move up and down.

Further, one end of the base close to the support frame is provided with a first sliding device for the sliding of the support frame, and the first sliding device includes a second slideway arranged on the base, which is arranged on the second slideway on the support frame. The slideway cooperates with the second sliding end provided.

Further, the base is also provided with a second sliding device for the support frame to slide left and right, the second sliding device includes a third slideway provided on the base, and is arranged on the support frame in cooperation with the third slideway The third sliding end of .

The beneficial effects of the application are as follows:

1. In this application, on the one hand, the positioning device can fix the position of the sample, and on the other hand, a stabilizer is installed in the positioning device, so that the sample is more stable when it is moving or rotating, and it is more conducive to the stability of imaging;

2. The main frame of the present application is equipped with a support frame on the base, making the whole structure more stable, and setting the fixing mechanism and positioning device on the support frame, making the whole device more convenient to use;

3. On the one hand, the arrangement of the elastic part in this application makes the rotation of the sample more stable after entering the channel, and there will be no shaking and other situations that affect the imaging effect; on the other hand, the device is suitable for samples of different sizes;

4. The setting of the cleaning part of this application enables the sample to be wiped on the surface of the sample by the cleaning part before imaging, which is more conducive to the clarity of imaging;

5. In this application, the matching liquid tank is installed in the corresponding position by using the clamping and fixing part;

6. This application utilizes the lifting device, the first sliding device, and the second sliding device so that the sample can be moved up and down, forward and backward, and left and right to adjust the position of the sample.

Description of drawings

The drawings described here are used to provide a further understanding of the application and constitute a part of the application. The schematic embodiments and descriptions of the application are used to explain the application and do not constitute an improper limitation to the application. In the attached picture:

Fig. 1 is the structural representation of a kind of sample stabilizing device in the present application;

Fig. 2 is the front view of the sample stabilizing device in Fig. 1;

Fig. 3 is the left side view of the sample stabilizing device in Fig. 1;

Fig. 4 is the structural representation of the stabilizer in the present application;

Fig. 5 is the structural representation of cleaning part in the present application;

Fig. 6 is a schematic structural diagram of the clamping and fixing part in the present application;

Fig. 7 is a structural schematic diagram of the lifting device in the present application.

Detailed ways

In order to explain the overall concept of the present application more clearly, the following detailed description will be given by way of examples in conjunction with the accompanying drawings.

In the following description, many specific details are set forth in order to fully understand the application, but the application can also be implemented in other ways different from those described here, therefore, the protection scope of the application is not limited by the specific details disclosed below. EXAMPLE LIMITATIONS.

In addition, in the description of the present application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal" ", "Top", "Bottom", "Inner", "Outer", "Axial", "Radial", "Circumferential" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings , is only for the convenience of describing the present application and simplifying the description, but does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present application.

In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of these features. In the description of the present application, "plurality" means two or more, unless otherwise specifically defined.

In this application, terms such as "installation", "connection", "connection" and "fixation" should be interpreted in a broad sense, for example, it may be a fixed connection or a detachable connection, unless otherwise clearly specified and limited. , or integrated; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection or an indirect connection through an intermediary, and it can be the internal communication of two components or the interaction relationship between two components . Those of ordinary skill in the art can understand the specific meanings of the above terms in this application according to specific situations.

In the present application, unless otherwise clearly specified and limited, a first feature being "on" or "under" a second feature may mean that the first and second features are in direct contact, or that the first and second features are indirect through an intermediary. touch. In the description of this specification, descriptions with reference to the terms "one embodiment", "some embodiments", "example", "specific examples", or "some examples" mean that specific features described in connection with the embodiment or example , structure, material or characteristic is included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the described specific features, structures, materials or characteristics may be combined in any suitable manner in any one or more embodiments or examples.

A sample stabilization device based on optical projection tomography, as shown in Figures 1-3, includes a main frame 1, a fixing mechanism 2 for fixing a sample 4 is arranged on the main frame 1, and a fixing mechanism 2 for fixing a sample 4 is arranged on the fixing mechanism 2 Rotating device, the main frame 1 is also provided with a positioning device 3, the positioning device 3 is arranged below the fixing mechanism 2, one end of the positioning device 3 is connected with the main frame 1, and the positioning device 3 includes a passage for the sample 4 to pass through. The hole 5, the positioning device 3 also includes a stabilizer 31, and the stabilizer 31 is arranged in cooperation with the through hole 5. The main frame 1 includes a base 11 and a support frame 12 , the support frame 12 is connected to the base 11 , and the support frame 12 is vertically arranged above the base 11 ; the fixing mechanism 2 and the positioning device 3 are both arranged on the support frame 12 . As shown in Figure 4, the stabilizer 31 includes a number of elastic pieces 311, one end of the elastic piece 311 is connected to the inner wall of the through hole 5, the number of elastic pieces 311 are evenly arranged in the through hole 5, and the other ends of the several elastic pieces 311 are matched to form the sample 4. through channel 312. As shown in FIG. 5 , several cleaning parts 313 for cleaning the outside of the sample 4 are provided inside the channel 312 . As shown in FIG. 6 , the base 11 is provided with a clamping and fixing part 7 for placing the matching liquid tank 6 , the clamping and fixing part 7 is arranged under the positioning device 3 , and the clamping and fixing part 7 is arranged in cooperation with the channel 312 . The clamping and fixing part 7 includes a plurality of clamping grooves 71 cooperating with the matching liquid tank 6 , and one end of the clamping grooves 71 is fixedly connected with the base 11 . Several locking protrusions are arranged inside the locking groove 71 . As shown in Figure 7, the main frame 1 is provided with a lifting device 8 for the lifting movement of the fixing mechanism 2. The lifting device 8 includes a first slideway 81 arranged on the support frame 12, and the fixing mechanism 2 is arranged to cooperate with the slideway. the first sliding end of . One end of the base 11 near the support frame 12 is provided with a first sliding device for the sliding of the support frame 12. The first sliding device includes a second slideway arranged on the base 11, and is arranged on the support frame 12 in cooperation with the second slideway. the second sliding end of . The base 11 is also provided with a second sliding device for the left and right sliding of the support frame 12. The second sliding device includes a third slideway arranged on the base 11, and a third slideway cooperating with the third slideway is arranged on the support frame 12. sliding end.

In this example, the sample is fixed in a capillary glass tube. If the sample size is large, the sample is stuck to one end of the capillary glass tube by adsorption. If the sample size is small, the sample is sealed in the capillary glass tube. The glass tube may be borosilicate material. Matching is also used to hold the matching liquid of titin, which is used to reduce the refraction and reflection effect of light on the surface of the organism, wherein the refractive index of the matching liquid should be close to the refractive index of the sample, and the matching liquid tank 6 is made of transparent borosilicate Glass support.

When this embodiment is in use, use the lifting device 8 to raise the fixing mechanism 2 to a suitable position, install the sample 4 on the fixing mechanism 2, fill the matching liquid tank 6 with an appropriate amount of matching liquid and put it into the clamping groove 71 In the process, the lifting device 8 is used to insert the sample 4 into the matching liquid tank 6 through the channel 312. If the imaging position is not suitable before the test, the position of the sample 4 can be adjusted by using the first sliding device and the second sliding device. .

It can be understood that the fixing mechanism 2 and the rotating device in this embodiment can adopt the mechanical movement device in the patent document CN102743159A. The fixing mechanism 2 is used to clamp the sample 4, and the rotating device is used to rotate the sample 4.

It can be understood that the elastic member 311 in the present application may be an elastic net, a spring, or a combination of an elastic element and a spring.

It can be understood that the cleaning part 313 in this application may be a flexible scraper or wiping cotton wrapped on the inner wall of the channel 312 . It is only necessary to wipe the component material on the outside of sample 4.

It can be understood that the lifting device 8 can also be configured to lift the entire support frame 12 .

Each embodiment in this specification is described in a progressive manner, the same and similar parts of each embodiment can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, as for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and for the related parts, please refer to the part of the description of the method embodiment.

The above are only examples of the present application, and are not intended to limit the present application. For those skilled in the art, various modifications and changes may occur in this application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the scope of the claims of the present application.

Claims (10)

1. a kind of sample securing device based on optical projection tomographic imaging, which is characterized in that including body frame, set on the body frame The fixed mechanism for fixing sample is set, the rotating device for sample rotates, the body frame are provided on the fixed mechanism On be additionally provided with positioning device, the positioning device is set to the lower section of the fixed mechanism, described positioning device one end and institute It states body frame to be connected, it further includes firmware in the positioning device that the positioning device, which includes the through-hole passed through for sample, described Firmware is equipped with the through-hole.

2. a kind of sample securing device based on optical projection tomographic imaging according to claim 1, which is characterized in that institute Stating body frame includes pedestal and support frame, and support frame as described above is connected with the pedestal, and support frame as described above is vertically installed in the pedestal Top；The fixed mechanism and the positioning device are arranged on support frame as described above.

3. a kind of sample securing device based on optical projection tomographic imaging according to claim 2, which is characterized in that institute Stating firmware includes several elastic components, and described elastic component one end is connected with the through-hole wall, and several elastic components are uniformly set It is placed in the through-hole, several elastic component other ends are equipped with to form the channel that the sample passes through.

4. a kind of sample securing device based on optical projection tomographic imaging according to claim 3, which is characterized in that institute It states and is provided with several cleaning portions for being used to clear up sample exterior on the inside of channel.

5. a kind of sample securing device based on optical projection tomographic imaging according to claim 3, which is characterized in that institute It states to be provided on pedestal and connects and fixes portion for placing matching liquid bath, the portion of connecting and fixing is set under the positioning device Side, the portion that connects and fixes is equipped with the channel.

6. a kind of sample securing device based on optical projection tomographic imaging according to claim 5, which is characterized in that institute Stating the portion of connecting and fixing includes several with the clamping groove for matching liquid bath and being equipped with, and described clamping groove one end and the pedestal are solid Even.

7. a kind of sample securing device based on optical projection tomographic imaging according to claim 6, which is characterized in that institute It states and is provided with several clamping bumps on the inside of clamping groove.

8. a kind of sample securing device based on optical projection tomographic imaging according to claim 2, which is characterized in that institute The lifting device that setting is moved up and down for fixed mechanism on body frame is stated, the lifting device includes being arranged in support frame as described above On the first slideway, the first sliding end that setting is equipped with first slideway on the fixed mechanism.

9. a kind of sample securing device based on optical projection tomographic imaging according to claim 2, which is characterized in that institute State the first carriage that pedestal is used to support frame sliding close to the setting of one end of support frame as described above, the first carriage packet The second slideway being set on the base is included, the second sliding end that the second slideway is equipped with is set on support frame as described above.

10. a kind of sample securing device based on optical projection tomographic imaging according to claim 2, which is characterized in that It is additionally provided on the pedestal and is used to support the second carriage that frame horizontally slips, second carriage includes that setting exists Third slideway on pedestal is set to the third sliding end that third slideway is equipped on support frame as described above.