CN209727807U - An optical projection tomography system - Google Patents

Abstract

The present application provides an optical projection tomography system, including: a light source module, a sample carrying module, a signal acquisition module and a control module, and also includes an adjustment device for adjusting the light source, the adjustment device is arranged in cooperation with the light source module, The light source module includes a housing, a light source outlet is provided on a side of the housing close to the sample carrying module, and the adjustment device is arranged on the light source outlet. In this application, the adjustment device is used to adjust the light source, and the brightness, direction, and illumination range of the light source irradiated on the sample can be adjusted according to the actual situation through the adjustment device, which is beneficial to obtain better imaging effects.

Description

An optical projection tomography system

technical field

The present application relates to the technical field of molecular imaging, in particular to an optical projection tomography system.

Background technique

Optical projection tomography (OPT) technology uses the characteristics of light to travel along a straight line in a small-sized organism, emits visible light to penetrate the sample, or uses a laser to excite the sample to generate fluorescence, and then uses a camera to collect projection views of the sample from multiple angles. Perform 3D imaging. Specifically, when performing optical projection tomography, the sample needs to be scanned from multiple angles. Generally, an electronically controlled turntable is used to rotate the sample step by step, and one or more projection images are collected for each rotation to an angle. The final data collected by the circular orbit scanning of the optical projection tomography system is a series of two-dimensional projection images of light passing through the sample at different angles, and three-dimensional images can be obtained through corresponding reconstruction algorithms. Through the above-mentioned technology, high-resolution, structural-functional integration, and radiation-free qualitative and quantitative research on small-scale living organisms at the cell level can be achieved, and real-time, non-invasive, and dynamic imaging of organisms can be realized.

An optical projection tomography system is disclosed in CN102743159A, including: a light source module, used to provide white light or laser for illuminating the sample; a sample carrying positioning module, used to adjust the position of the sample and drive the sample to rotate to collect projections from multiple angles Data; the signal acquisition module is used to realize the convergence of optical signals and selectively collect optical signals; the central control module is used to coordinate the orderly operation of various parts, realize the opening and closing and intensity adjustment of the light source, and the center of the translation platform Calibration, parameter setting of the rotary table, parameter setting of the EMCCD camera, data processing and data storage; peripheral modules of the equipment are used to provide an anti-interference and stable environment for imaging. This application did not explore the illumination factors such as the illumination angle, illumination range, and light source brightness of the light source module, but the illumination has a great influence on the imaging result.

Utility model content

In order to solve the above technical problems, the present application provides an optical projection tomography system, including: a light source module, a sample carrying module, a signal acquisition module and a control module, and also includes an adjustment device for adjusting the light source, the adjustment device and the The light source module is cooperatingly arranged, and the light source module includes a housing, and a light source outlet is arranged on a side of the housing close to the sample carrying module, and the adjustment device is arranged on the light source outlet. The light source module in this application is used to provide a light source for irradiating the sample; the sample carrying module is used for the installation of the sample and the adjustment of the sample position; the signal acquisition module is used for the convergence of optical signals and the selective acquisition of optical signals; The orderly operation of components, as well as data processing and data storage. In this application, the adjustment device is used to adjust the light source, and the brightness, direction, and illumination range of the light source irradiated on the sample can be adjusted according to the actual situation through the adjustment device, which is beneficial to obtain better imaging effects.

Further, the adjustment device includes a moving plate arranged on the light source outlet, the two sides of the moving plate are connected to the housing, and the housing is provided with a chute for the moving plate to slide up and down, and the moving plate Light-transmitting holes with different cross-sectional areas are arranged on the top. In this application, by moving the position of the plate, part of the light is blocked to adjust the light source, and the setting of the through hole is used to adjust the range of the light and the adjustment of the range of the light.

Further, the adjustment device includes a baffle arranged at the outlet of the light source, both ends of the baffle are hingedly connected to the housing through connecting pieces, the baffle is set on a curved surface, and several Light transmission holes with different illumination angles. The baffle can move up and down at the exit of the light source, and can also block the light to adjust the brightness of the light source. The curved surface of the baffle is designed to gather or disperse the light source according to actual needs. Light range and light direction can be adjusted.

Further, the light source module includes a laser device, and the laser device is provided with a laser emitting part and a laser processing part in sequence according to the laser emitting direction, and the laser processing part is sequentially provided with a beam homogenization device and a laser processing part according to the laser emitting direction. Beam collimator. The laser emitting part is used to emit laser light, and the beam homogenization device is used to eliminate the inherent astigmatism of the beam in the transmission direction, and perform a certain homogenization treatment on the spot. The beam homogenization device can use frosted glass homogenization sheet, engineering scattering sheet or beam Homogenizing sheet, etc.; the laser collimating device makes the emitted laser light more collimated. The laser collimating device can use positive and negative lenses or ordinary lenses with aspheric or free-form surfaces to shape the beam after passing through the beam homogenizing device. .

Further, the beam homogenizing device includes several beam homogenizing sheets, and the beam collimating device includes several beam collimating lenses and beam focusing lenses.

Further, the light source module also includes a support table for fixing the support shell, and also includes a control device for the movement of the support table, the control device includes a telescopic part for the support table to perform telescopic movement, and also includes a The lifting part that does the lifting movement. In this application, the position of the light source module can be adjusted as required, the light source module can be moved back and forth by using the telescopic part, and the light source module can be moved up and down by using the lifting part.

Further, the sample carrying module includes a main frame, the main frame is provided with a fixing mechanism for fixing the sample, the fixing mechanism is provided with a rotating device for sample rotation, and the main frame is also provided with a positioning device , the positioning device is arranged below the fixing mechanism, one end of the positioning device is connected to the main frame, the positioning device includes a through hole for the sample to pass through, and the positioning device also includes a stabilizer, The stabilizer is set in cooperation with the through hole. The fixing mechanism in this application is used to fix the sample in this application. The rotating device in the fixing mechanism is used for the rotation of the sample. On the one hand, the positioning device plays a role in positioning the position of the sample. Components make the sample more stable when moving or rotating, which is more conducive to the stability of imaging.

Further, the stabilizer includes several elastic pieces, one end of which is connected to the inner wall of the through hole, the several elastic pieces are evenly arranged in the through hole, and the other ends of the several elastic pieces are matched to form the inner wall of the through hole. The channel through which the sample passes, and several cleaning parts for cleaning the outside of the sample are arranged inside the channel. On the one hand, the setting of the elastic member makes the rotation of the sample more stable after entering the channel, and there will be no shaking and other conditions that affect the imaging effect; on the other hand, the device is suitable for samples of different sizes. The surface of the sample is wiped by the cleaning part before imaging, which is more conducive to the clarity of the image; on the other hand, if the sample is placed in the matching liquid tank, after the test is completed, the surface of the sample is covered with matching liquid. If it is not wiped off in time , may affect the operation of electronic components.

Further, the main frame includes a base and a support frame, the support frame is connected to the base, and the support frame is vertically arranged above the base; the fixing mechanism and the positioning device are both arranged on the support on the shelf. The main frame of the present application is provided with a support frame on the base to make the whole structure more stable, and the fixing mechanism and positioning device are arranged on the support frame to make the whole device more convenient to use.

Further, the base is provided with a clamping and fixing part for placing a matching liquid tank, the clamping and fixing part is arranged under the positioning device, and the clamping and fixing part is arranged in cooperation with the channel. The installation and fixation of the matching liquid tank is also very important for the stability of the imaging. In this application, the clamping and fixing part is used to install the matching liquid tank at a corresponding position.

The beneficial effects of the application are as follows:

1. In this application, the adjustment device is used to adjust the light source. The brightness, direction and illumination range of the light source irradiated on the sample can be adjusted according to the actual situation through the adjustment device, which is beneficial to obtain better imaging effect;

2. This application blocks part of the light by moving the position of the plate to adjust the light source, and the setting of the through hole is used to adjust the light transmission range and the adjustment of the transmission range;

3. The baffle of this application can move up and down at the exit of the light source, and can also block the light to adjust the brightness of the light source. The curved surface of the baffle is set to gather or disperse the light source according to actual needs. The setting of the through hole is In order to adjust the illumination range and illumination direction of the light source;

4. In this application, the position of the light source module can be adjusted according to the needs. The telescopic part can be used to make the light source module move forward and backward, and the lifting part can be used to make the light source module move up and down;

5. On the one hand, the positioning device in this application plays a positioning role in fixing the position of the sample, and on the other hand, a stabilizer is provided in the positioning device to make the sample more stable when moving or rotating, which is more conducive to the stability of imaging;

6. The setting of the elastic part in this application makes the rotation of the sample more stable after entering the channel on the one hand, and there will be no shaking and other situations that will affect the imaging effect

Description of drawings

The drawings described here are used to provide a further understanding of the application and constitute a part of the application. The schematic embodiments and descriptions of the application are used to explain the application and do not constitute an improper limitation to the application. In the attached picture:

Fig. 1 is a schematic structural diagram of an optical projection tomography system in the present application;

Fig. 2 is the structural representation of support platform in the present application;

FIG. 3 is a schematic structural diagram of a light source module in the present application;

Fig. 4 is the structural representation of the sample carrying module in the present application;

Fig. 5 is the structural representation of the stabilizer in the present application;

Fig. 6 is the structural representation of cleaning part in the present application;

Fig. 7 is a schematic structural diagram of the clamping and fixing part in the present application;

Fig. 8 is a schematic structural diagram of the mobile plate in the present application;

Fig. 9 is a schematic structural diagram of the regulating device in the present application.

Detailed ways

In order to explain the overall concept of the present application more clearly, the following detailed description will be given by way of examples in conjunction with the accompanying drawings.

In the following description, many specific details are set forth in order to fully understand the application, but the application can also be implemented in other ways different from those described here, therefore, the protection scope of the application is not limited by the specific details disclosed below. EXAMPLE LIMITATIONS.

In addition, in the description of the present application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal" ", "Top", "Bottom", "Inner", "Outer", "Axial", "Radial", "Circumferential" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings , is only for the convenience of describing the present application and simplifying the description, but does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present application.

In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of these features. In the description of the present application, "plurality" means two or more, unless otherwise specifically defined.

In this application, terms such as "installation", "connection", "connection" and "fixation" should be interpreted in a broad sense, for example, it may be a fixed connection or a detachable connection, unless otherwise clearly specified and limited. , or integrated; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection or an indirect connection through an intermediary, and it can be the internal communication of two components or the interaction relationship between two components . Those of ordinary skill in the art can understand the specific meanings of the above terms in this application according to specific situations.

In the present application, unless otherwise clearly specified and limited, a first feature being "on" or "under" a second feature may mean that the first and second features are in direct contact, or that the first and second features are indirect through an intermediary. touch. In the description of this specification, descriptions with reference to the terms "one embodiment", "some embodiments", "example", "specific examples", or "some examples" mean that specific features described in connection with the embodiment or example , structure, material or characteristic is included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the described specific features, structures, materials or characteristics may be combined in any suitable manner in any one or more embodiments or examples.

An optical projection tomography system in this embodiment, as shown in Figure 1 and Figure 2, includes: a light source module 1, a sample carrying module 2, a signal acquisition module 3 and a control module, and also includes an adjustment device for adjusting the light source 5. The adjustment device 5 is arranged in cooperation with the light source module 1. The light source module 1 includes a housing 11. The side of the housing 11 close to the sample carrying module 2 is provided with a light source outlet 12. The adjustment device 5 is arranged on the light source outlet 12.

As shown in Fig. 3, the light source module 1 includes a laser device 13. In the laser device 13, a laser emitting part 131 and a laser processing part 132 are sequentially arranged according to the laser emitting direction. Lightning device 133 and beam collimating device 134. The beam homogenizing device 133 includes several beam homogenizing sheets 1331 , and the beam collimating device 134 includes several beam collimating lenses 1332 and beam focusing lenses 1333 .

As shown in FIG. 2 , the light source module 1 further includes a support table 14 for fixing the support shell 11, and a control device for the movement of the support table 14. The control device includes a telescopic part for the support table 14 to perform telescopic movement, and also includes The lifting part is used for supporting the platform 14 to move up and down.

As shown in Figure 4, the sample carrying module 2 includes a main frame 21, a fixing mechanism 22 for fixing the sample is arranged on the main frame 21, a rotating device for sample rotation is arranged on the fixing mechanism 22, and a positioning device is also arranged on the main frame 21. Device 23, the positioning device 23 is arranged below the fixing mechanism 22, one end of the positioning device 23 is connected with the main frame 21, the positioning device 23 includes a through hole 231 for the sample to pass, and the positioning device 23 also includes a stabilizer 24, a stabilizer 24 is set in cooperation with the through hole 231. As shown in Figure 5, the stabilizer 24 includes a number of elastic pieces 241, one end of the elastic piece 241 is connected to the inner wall of the through hole 231, the number of elastic pieces 241 are evenly arranged in the through hole 231, and the other ends of the several elastic pieces 241 are matched to form a sample through the hole. As shown in FIG. 6 , several cleaning parts 243 for cleaning the outside of the sample are provided inside the channel 242 .

As shown in Figure 7, the main frame 21 includes a base 211 and a support frame 212, the support frame 212 is connected to the base 211, and the support frame 212 is vertically arranged above the base 211; the fixing mechanism 22 and the positioning device 23 are all arranged on the support frame 212. The base 211 is provided with a clamping and fixing part 213 for placing the matching liquid tank 25 , the clamping and fixing part 213 is arranged under the positioning device 23 , and the clamping and fixing part 213 is arranged in cooperation with the channel 242 .

It can be understood that sample 26 in this embodiment uses a capillary glass tube to fix the sample. If the sample size is large, the sample is stuck to one end of the capillary glass tube by adsorption. If the sample size is small, the sample is sealed in the capillary glass tube. Of the glass tubes, the capillary glass tube may be a borosilicate material. Matching is also used to hold the matching liquid of titin, which is used to reduce the refraction and reflection effects of light on the surface of the organism, wherein the refractive index of the matching liquid should be close to the refractive index of the sample, and the matching liquid tank 25 is made of transparent borosilicate Glass support.

It can be understood that the light source module 1 includes a halogen lamp, and the light source module 1 is composed of a halogen lamp and a laser; wherein, the halogen lamp emits white light for structural imaging of biological samples; the laser emits laser light for specific imaging of biological samples; White light or laser light is exported to the designated position through optical fiber.

It can be understood that the signal acquisition module 3 in this application can adopt the signal acquisition module 3 in CN102743159A, and the control module 4 can adopt the central control module 4 in CN102743159A.

It can be understood that, as shown in FIG. 8 , the adjustment device 5 includes a moving plate 51 arranged on the light source outlet 12 , the two sides of the moving plate 51 are connected with the casing 11 , and the casing 11 is provided with a chute 52 for the moving plate 51 to slide up and down. , the moving plate 51 is provided with light-transmitting holes 53 with different cross-sectional areas.

It can be understood that, as shown in FIG. 9 , the adjustment device 5 includes a baffle 54 arranged at the light source outlet 12 , the two ends of the baffle 54 are hingedly connected with the housing 11 through a connecting piece 55 , and the baffle 54 is arranged on a curved surface. Several light-transmitting holes 53 with different cross-sectional areas are provided.

It can be understood that the light-transmitting hole 53 is divided into an upper half and a lower half. The upper half of the light-transmitting hole opens upwards, and the angle with the horizontal is 10-30°, and the opening of the lower half of the light-transmitting hole faces downwards, and the angle with the horizontal is 10-30°. 10-30, this setting makes the emitted light range wider.

It can be understood that the telescopic part may adopt structures such as a connecting rod, a sliding block on a slide rail, a screw rod, or a piston cylinder.

It can be understood that the lifting part can adopt structures such as a connecting rod, a sliding block on a slide rail, a screw rod, and a piston cylinder.

It can be understood that the fixing mechanism 22 and the rotating device in this embodiment can adopt the mechanical movement device in the patent document CN102743159A. The fixing mechanism 22 is used to clamp the sample 26, and the rotating device is used to rotate the sample 26.

It can be understood that the elastic member 241 in this application may be an elastic net, a spring or a combination of an elastic element and a spring.

It can be understood that the cleaning part 243 in this application may be a flexible scraper or wiping cotton wrapped on the inner wall of the channel 242 . It is sufficient to wipe the component material on the outside of the sample 26 .

It can be understood that the lifting device can also be configured to lift the entire support frame 212 .

The operation of the optical projection tomography system includes the following:

Step 1: first start the power supply of an optical projection tomography system of the present application, use the lifting device to raise the fixing mechanism 22 to a suitable position, install the sample 26 on the fixing mechanism 22, and put an appropriate amount of The matching solution is put into the clamping tank, and then the sample 26 is inserted into the matching solution tank 25 through the channel 242 by using the lifting device.

Step 2: According to the real-time images collected by the camera, if the imaging position is not suitable before the test, the position of the sample 26 can be adjusted by using the first sliding device and the second sliding device, so that the sample is just near the imaging lens, and the adjustment device can be used to 5. The telescopic part and the lifting part adjust the light source to make the image clearest.

Step 3: Set the parameters of the rotating device, including step angle, angular displacement, angular velocity and waiting time after each rotation step. Set the working parameters of the camera, including exposure time and shooting interval. Select the imaging mode, when the structural imaging is selected, the white light is turned on and the laser is turned off; when the specific imaging is selected, the laser is turned on and the white light is turned off. At this point, the parameters are set.

Step 4: Image acquisition, at this time all parts work in harmony. The captured images are displayed in the software display area. And automatically save to the specified location. After the image is collected, the system automatically processes the obtained projection image, reconstructs the three-dimensional structure of the image, and outputs the reconstructed result. So far, this data processing is completed. Turn off each electric control device in the dark box in turn, and finally turn off the workstation.

Each embodiment in this specification is described in a progressive manner, the same and similar parts of each embodiment can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, as for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and for the related parts, please refer to the part of the description of the method embodiment.

The above descriptions are only examples of the present application, and are not intended to limit the present application. For those skilled in the art, various modifications and changes may occur in this application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the scope of the claims of the present application.

Claims (10)

1. An optical projection tomography system, comprising: a light source module, a sample carrying module, a signal acquisition module and a control module, characterized in that, it also includes an adjustment device for adjusting the light source, and the adjustment device cooperates with the light source module It is provided that the light source module includes a housing, a light source outlet is provided on a side of the housing close to the sample carrying module, and the adjustment device is arranged on the light source outlet. 2. An optical projection tomography system according to claim 1, wherein the adjusting device comprises a moving plate arranged on the light source outlet, the two sides of the moving plate are connected to the housing, and the housing A chute for sliding up and down of the moving plate is arranged on the top, and light transmission holes with different cross-sectional areas are arranged on the moving plate. 3. An optical projection tomography system according to claim 1, wherein the adjusting device comprises a baffle arranged at the outlet of the light source, and both ends of the baffle are hinged to the housing through connecting pieces connected, the baffle is set on a curved surface, and the baffle is provided with several light-transmitting holes with different irradiation angles. 4. A kind of optical projection tomography system according to claim 1, characterized in that, the light source module comprises a laser device, a laser emitting part and a laser processing part are sequentially arranged in the laser device according to the laser emitting direction, so A beam homogenizing device and a beam collimating device are sequentially arranged in the laser processing part according to the laser emitting direction. 5. A kind of optical projection tomography system according to claim 4, it is characterized in that, said beam homogenization device comprises several beam homogenization sheets, and said beam collimation device comprises several beam collimating lenses and beam focusing lens. 6. An optical projection tomography system according to claim 1, wherein the light source module further comprises a support table for fixing the support shell, and also includes a control device for the movement of the support table, the control device It includes a telescopic part for the supporting table to perform telescopic movement, and also includes a lifting part for the supporting table to perform lifting movement. 7. An optical projection tomography system according to claim 1, wherein the sample carrying module comprises a main frame, a fixing mechanism for fixing the sample is arranged on the main frame, and a fixing mechanism is arranged on the fixing mechanism There is a rotating device for sample rotation, and the main frame is also provided with a positioning device, the positioning device is arranged below the fixing mechanism, one end of the positioning device is connected with the main frame, and the positioning device includes For the through hole through which the sample passes, the positioning device further includes a stabilizer, and the stabilizer is arranged in cooperation with the through hole. 8. An optical projection tomography system according to claim 7, wherein the stabilizer includes several elastic members, one end of the elastic member is connected to the inner wall of the through hole, and the plurality of elastic members are evenly arranged In the through hole, the other ends of the plurality of elastic members cooperate to form a channel through which the sample passes, and a plurality of cleaning parts for cleaning the outside of the sample are arranged inside the channel. 9. An optical projection tomography system according to claim 8, wherein the main frame comprises a base and a support frame, the support frame is connected to the base, and the support frame is vertically arranged on the Above the base; both the fixing mechanism and the positioning device are arranged on the support frame. 10. An optical projection tomography system according to claim 9, characterized in that, the base is provided with a clamping and fixing part for placing a matching liquid tank, and the clamping and fixing part is arranged on the positioning device Below, the clamping and fixing part is arranged in cooperation with the channel.