Automatic sampling device of fermentation tank
Technical Field
The utility model relates to the technical field of biological fermentation equipment, in particular to an automatic sampling device of a fermentation tank.
Background
At the moment of the vigorous development of the biological fermentation industry, the fermentation process is widely applied to various fields such as foods, medicines, biological energy sources, agricultural biological preparations and the like. The fermenter is used as a core device in the biological fermentation process and plays an important role in providing a suitable environment for the growth and metabolism of microorganisms and promoting the microorganisms to convert the raw materials into target fermentation products. The fermentation process is a complex dynamic system, and the processes of growth and propagation of microorganisms, generation and accumulation of metabolites and the like are continuously evolved along with the change of fermentation time and environmental conditions.
In order to accurately master the fermentation process, ensure that the fermentation process is carried out according to the expected direction, finally obtain the fermentation product with high quality and high yield, and the real-time and accurate sampling detection of the fermentation liquor in the fermentation tank is an essential link. Through periodic sampling analysis, key parameters such as the quantity and the type of microorganisms in fermentation liquor, the concentration and the type of metabolites, the substrate consumption condition and the like can be known in time, so that scientific basis is provided for optimizing and adjusting the fermentation process, for example, the operation conditions such as fermentation temperature, pH value, dissolved oxygen amount, feeding rate and the like are reasonably controlled.
However, in the prior art, the sampling mode adopts manual sampling, the fermentation process is required to be frequently interrupted, and the risk of bacteria contamination is increased.
Disclosure of utility model
The utility model aims to provide an automatic sampling device for a fermentation tank, and aims to solve the technical problems that in the prior art, a sampling mode adopts manual sampling, the fermentation process is required to be frequently interrupted, and the risk of bacteria contamination is increased.
In order to achieve the above purpose, the automatic sampling device for the fermentation tank comprises a sampling tube, an electric diaphragm valve, a peristaltic pump, a connecting tube, a controller, an air inlet assembly and a plurality of groups of sampling assemblies, wherein the sampling tube is arranged in the fermentation tank to be sampled, one end of the connecting tube is connected with the sampling tube through the peristaltic pump, the electric diaphragm valve is arranged on the sampling tube, the air inlet assembly and the plurality of groups of sampling assemblies are connected with the connecting tube, and the controller is connected with the electric diaphragm valve, the peristaltic pump, the air inlet assembly and the plurality of groups of sampling assemblies.
The air inlet assembly comprises an air inlet pipe and an air pinch valve, the air inlet pipe is communicated with the connecting pipe, and the air pinch valve is arranged on the air inlet pipe.
Wherein, the subassembly that admits air still includes the air degerming filter, the air degerming filter sets up in the intake pipe.
The sampling assembly comprises a silicone tube, a quick connector and a sampling bottle, one end of the silicone tube is communicated with the connecting tube, a discharge pinch valve is arranged on the silicone tube, and the sampling bottle is connected with the silicone tube through the quick connector.
The automatic sampling device for the fermentation tank further comprises a water bath kettle with a cooling function, and the water bath kettle with the cooling function is used for placing a plurality of sampling bottles.
The automatic sampling device for the fermentation tank is characterized in that a sampling period and a sampling amount are set in a controller in advance when the automatic sampling device is specifically used, the electric diaphragm valve is opened, compressed air is blown to the sampling tube through the air inlet component, fermentation liquor in the electric diaphragm valve is blown out, so that sampled samples represent real conditions at the time, after the set time is reached, the air inlet component is closed, one of the sampling tubes is opened corresponding to the pinch valve, the peristaltic pump is opened, fermentation liquor in the fermentation tank enters the corresponding sampling tube under the action of the peristaltic pump along the sampling tube, the electric diaphragm valve, the connecting tube and the discharge pinch valve, meanwhile, the peristaltic pump calculates an accumulated value, after the peristaltic pump is accumulated to a set value, the electric diaphragm valve is closed, the air inlet component is opened, the fermentation liquor after the connecting tube is pressed into the corresponding sampling tube, then the discharge pinch valve is closed, the electric diaphragm valve is opened, the air inlet component opens the corresponding pinch valve, the peristaltic pump is opened, the peristaltic pump is closed, the sampling liquor in the sampling tube is emptied by the sampling tube, and the whole fermentation tank is emptied by the manual sampling device, and the problem of the sampling device is solved, and the problem of the conventional sampling device is solved, and the problem of frequently-like is solved, and the problem of sampling is solved.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an automatic sampling device for a fermenter according to the present utility model.
1-Sampling tube, 2-electric diaphragm valve, 3-peristaltic pump, 4-connecting tube, 5-controller, 6-silicone tube, 7-quick connector, 8-sampling bottle, 9-discharge pinch valve, 10-intake pipe, 11-air pinch valve, 12-air degerming filter, 13-water bath with cooling function, 14-fermentation tank.
Detailed Description
The following detailed description of embodiments of the utility model, examples of which are illustrated in the accompanying drawings and, by way of example, are intended to be illustrative, and not to be construed as limiting, of the utility model.
Referring to fig. 1, fig. 1 is a schematic diagram of an automatic sampling device for a fermenter 14 according to the present utility model.
The utility model provides an automatic sampling device of a fermentation tank 14, which comprises a sampling tube 1, an electric diaphragm valve 2, a peristaltic pump 3, a connecting tube 4, a controller 5, an air inlet component and a plurality of groups of sampling components, wherein the sampling tube 1 is arranged in the fermentation tank 14 to be sampled, one end of the connecting tube 4 is connected with the sampling tube 1 through the peristaltic pump 3, the electric diaphragm valve 2 is arranged on the sampling tube 1, the air inlet component and the plurality of groups of sampling components are connected with the connecting tube 4, and the controller 5 is connected with the electric diaphragm valve 2, the peristaltic pump 3, the air inlet component and the plurality of groups of sampling components;
The sampling assembly comprises a silicone tube 6, a quick connector 7 and a sampling bottle 8, one end of the silicone tube 6 is communicated with the connecting tube 4, a discharge pinch valve 9 is arranged on the silicone tube 6, and the sampling bottle 8 is connected with the silicone tube 6 through the quick connector 7.
For the specific implementation mode, when the device is specifically used, sampling period and sampling quantity are set in the controller 5 in advance, the electric diaphragm valve 2 is opened, compressed air is blown to the sampling tube 1 through the air inlet component, fermentation liquor in the electric diaphragm valve 2 is blown out, so that sampled samples represent real conditions at the time, after the set time is reached, the air inlet component is closed, one of the sampling tubes 8 is opened corresponding to the pinch valve, the peristaltic pump 3 is opened, fermentation liquor in the fermentation tank 14 enters the corresponding sampling tube 8 along the sampling tube 1, the electric diaphragm valve 2, the connecting tube 4 and the discharge pinch valve 9 under the action of the peristaltic pump 3, the peristaltic pump 3 calculates accumulated values, after the peristaltic pump 3 is accumulated to a set value, the electric diaphragm valve 2 is closed, the air inlet component is opened, the fermentation liquor after the 4 is pressed into the corresponding sampling tube 8, then the discharge pinch valve 9 is closed, the electric diaphragm valve 2 is opened, the sampling tube 1 is closed, the sampling tube is stopped, and the sampling tube is stopped, the whole fermentation liquor is emptied by the sampling tube is stopped, and the problems of the conventional sampling device are solved, and the conventional sampling device is solved, and the problems of the conventional sampling device are solved, and the sampling is frequently, and the sampling of the fermentation tube is completely emptied.
Wherein, the air intake assembly comprises an air intake pipe 10 and an air pinch valve 11, the air intake pipe 10 is communicated with the connecting pipe 4, and the air pinch valve 11 is arranged on the air intake pipe 10.
For the present embodiment, the air inlet pipe 10 is used for delivering air into the sampling pipe 1 and the connecting pipe 4, and the air pinch valve 11 is used for controlling the opening and closing of the air inlet pipe 10.
Next, the intake assembly further includes an air sterilization filter 12, and the air sterilization filter 12 is disposed on the intake pipe 10.
For the present embodiment, the air sterilizing filter 12 is used for filtering and sterilizing the air entering the sampling tube 1 and the connecting tube 4.
Simultaneously, fermentation cylinder 14 automatic sampling device still includes the water bath 13 of area cooling function, the water bath 13 of area cooling function is used for placing many the sample bottle 8.
For this embodiment, the water bath 13 with the cooling function is used for placing a plurality of sampling bottles 8, and keeps the sampled samples below 4 ℃ all the time for storage, so that the integrity of the samples in the storage time can be ensured.
When the automatic sampling device for the fermentation tank 14 is used, a sampling period and a sampling amount are set in the controller 5 in advance, the electric diaphragm valve 2 is opened, compressed air is blown to the sampling tube 1 through the air inlet component, and fermentation liquor in the electric diaphragm valve 2 is blown out, so that the sampled sample represents the real condition at the time, after the set time is reached, the air inlet component is closed, one of the sampling tube valves 8 is opened corresponding to the pinch valve, the peristaltic pump 3 is opened, fermentation liquor in the fermentation tank 14 enters the corresponding sampling tube 8 along the electric diaphragm valve 2, the connecting tube 4 and the discharge pinch valve 9 under the action of the peristaltic pump 3, the peristaltic pump 3 calculates an accumulated value, after the accumulated value of the peristaltic pump 3 is up to a set value, the electric diaphragm valve 2 is closed, the air inlet component is opened, the fermentation liquor in the back direction is pressed into the corresponding sampling tube 8, then the discharge pinch valve 9 is closed, the sampling tube valve 2 is closed, and the whole fermentation liquor in the sampling tube is completely emptied by the manual sampling tube is completely, and the problem of the fermentation tank is solved by adopting the conventional method, and the manual sampling device is completely emptied by the method of the sampling tube valve 1.
The above disclosure is only a preferred embodiment of the present utility model, and it should be understood that the scope of the utility model is not limited thereto, and those skilled in the art will appreciate that all or part of the procedures described above can be performed according to the equivalent changes of the claims, and still fall within the scope of the present utility model.