CN85108564B - Fermentation of Aspergillus niger for coproduction of diosgenin and citric acid - Google Patents
Fermentation of Aspergillus niger for coproduction of diosgenin and citric acid Download PDFInfo
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- CN85108564B CN85108564B CN85108564A CN85108564A CN85108564B CN 85108564 B CN85108564 B CN 85108564B CN 85108564 A CN85108564 A CN 85108564A CN 85108564 A CN85108564 A CN 85108564A CN 85108564 B CN85108564 B CN 85108564B
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- fermentation
- diosgenin
- citric acid
- saponin
- yield
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 238000000855 fermentation Methods 0.000 title claims abstract description 39
- 230000004151 fermentation Effects 0.000 title claims abstract description 39
- WQLVFSAGQJTQCK-UHFFFAOYSA-N diosgenin Natural products CC1C(C2(CCC3C4(C)CCC(O)CC4=CCC3C2C2)C)C2OC11CCC(C)CO1 WQLVFSAGQJTQCK-UHFFFAOYSA-N 0.000 title claims abstract description 32
- DWCSNWXARWMZTG-UHFFFAOYSA-N Trigonegenin A Natural products CC1C(C2(CCC3C4(C)CCC(O)C=C4CCC3C2C2)C)C2OC11CCC(C)CO1 DWCSNWXARWMZTG-UHFFFAOYSA-N 0.000 title claims abstract description 27
- WQLVFSAGQJTQCK-VKROHFNGSA-N diosgenin Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)CC[C@H](O)CC4=CC[C@H]3[C@@H]2C1)C)[C@@H]1C)[C@]11CC[C@@H](C)CO1 WQLVFSAGQJTQCK-VKROHFNGSA-N 0.000 title claims abstract description 27
- 241000228245 Aspergillus niger Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 5
- 229930182490 saponin Natural products 0.000 claims description 19
- 150000007949 saponins Chemical class 0.000 claims description 19
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 18
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 235000004879 dioscorea Nutrition 0.000 abstract description 11
- 229920002472 Starch Polymers 0.000 abstract description 8
- 230000007062 hydrolysis Effects 0.000 abstract description 8
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 8
- 235000019698 starch Nutrition 0.000 abstract description 8
- 239000008107 starch Substances 0.000 abstract description 8
- 239000002253 acid Substances 0.000 abstract description 4
- 235000005903 Dioscorea Nutrition 0.000 abstract description 2
- 235000000504 Dioscorea villosa Nutrition 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- HDXIQHTUNGFJIC-UHFFFAOYSA-N (25R)-spirost-5-en-3beta-ol 3-O-<O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside> Natural products O1C2(OCC(C)CC2)C(C)C(C2(CCC3C4(C)CC5)C)C1CC2C3CC=C4CC5OC1OC(CO)C(O)C(O)C1OC1OC(C)C(O)C(O)C1O HDXIQHTUNGFJIC-UHFFFAOYSA-N 0.000 abstract 2
- VNONINPVFQTJOC-RXEYMUOJSA-N Collettiside III Natural products O([C@@H]1[C@@H](O)[C@H](O[C@H]2[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O2)[C@H](CO)O[C@@H]1O[C@@H]1CC=2[C@@](C)([C@@H]3[C@H]([C@H]4[C@@](C)([C@H]5[C@H](C)[C@@]6(O[C@H]5C4)OC[C@H](C)CC6)CC3)CC=2)CC1)[C@H]1[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O1 VNONINPVFQTJOC-RXEYMUOJSA-N 0.000 abstract 2
- VNONINPVFQTJOC-ZGXDEBHDSA-N dioscin Chemical compound O([C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)O[C@H]1[C@@H]([C@H](O)[C@@H](O)[C@H](C)O1)O)O[C@@H]1CC2=CC[C@H]3[C@@H]4C[C@H]5[C@@H]([C@]4(CC[C@@H]3[C@@]2(C)CC1)C)[C@@H]([C@]1(OC[C@H](C)CC1)O5)C)[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O VNONINPVFQTJOC-ZGXDEBHDSA-N 0.000 abstract 2
- CJNUQCDDINHHHD-APRUHSSNSA-N dioscin Natural products C[C@@H]1CC[C@@]2(OC1)O[C@H]3C[C@H]4[C@@H]5CC=C6C[C@H](CC[C@@H]6[C@H]5CC[C@]4(C)[C@H]3[C@@H]2C)O[C@@H]7O[C@H](CO)[C@@H](O[C@@H]8O[C@@H](C)[C@H](O)[C@@H](O)[C@H]8O)[C@H](O)[C@H]7O[C@@H]9O[C@@H](C)[C@H](O)[C@@H](O)[C@H]9O CJNUQCDDINHHHD-APRUHSSNSA-N 0.000 abstract 2
- VNONINPVFQTJOC-UHFFFAOYSA-N polyphyllin III Natural products O1C2(OCC(C)CC2)C(C)C(C2(CCC3C4(C)CC5)C)C1CC2C3CC=C4CC5OC(C(C1O)OC2C(C(O)C(O)C(C)O2)O)OC(CO)C1OC1OC(C)C(O)C(O)C1O VNONINPVFQTJOC-UHFFFAOYSA-N 0.000 abstract 2
- 241000234273 Dioscorea Species 0.000 abstract 1
- 239000007791 liquid phase Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 235000017709 saponins Nutrition 0.000 description 17
- 235000007056 Dioscorea composita Nutrition 0.000 description 10
- 244000281702 Dioscorea villosa Species 0.000 description 9
- 235000002723 Dioscorea alata Nutrition 0.000 description 8
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 description 8
- 235000005362 Dioscorea floribunda Nutrition 0.000 description 8
- 235000004868 Dioscorea macrostachya Nutrition 0.000 description 8
- 235000005361 Dioscorea nummularia Nutrition 0.000 description 8
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- 238000005903 acid hydrolysis reaction Methods 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- NWMIYTWHUDFRPL-UHFFFAOYSA-N sapogenin Natural products COC(=O)C1(CO)C(O)CCC2(C)C1CCC3(C)C2CC=C4C5C(C)(O)C(C)CCC5(CCC34C)C(=O)O NWMIYTWHUDFRPL-UHFFFAOYSA-N 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 240000003183 Manihot esculenta Species 0.000 description 4
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 4
- 238000009835 boiling Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 235000013312 flour Nutrition 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 235000002722 Dioscorea batatas Nutrition 0.000 description 3
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 3
- 240000001811 Dioscorea oppositifolia Species 0.000 description 3
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000002329 infrared spectrum Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 244000080208 Canella winterana Species 0.000 description 2
- 235000008499 Canella winterana Nutrition 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 235000010240 Paullinia pinnata Nutrition 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 150000002338 glycosides Chemical group 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- JUVIOZPCNVVQFO-HBGVWJBISA-N rotenone Chemical compound O([C@H](CC1=C2O3)C(C)=C)C1=CC=C2C(=O)[C@@H]1[C@H]3COC2=C1C=C(OC)C(OC)=C2 JUVIOZPCNVVQFO-HBGVWJBISA-N 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 240000000530 Alcea rosea Species 0.000 description 1
- 235000017334 Alcea rosea Nutrition 0.000 description 1
- 235000017303 Althaea rosea Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101100433727 Caenorhabditis elegans got-1.2 gene Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000908494 Dioscorea nipponica Species 0.000 description 1
- 235000017008 Dioscorea nipponica Nutrition 0.000 description 1
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- JLISZLJGTVNTPC-UBWBUNFISA-N Yuccagenin Natural products C[C@@H]1CC[C@@]2(OC1)O[C@H]3C[C@H]4[C@@H]5CCC6=C[C@@H](O)[C@H](O)C[C@]6(C)[C@H]5CC[C@]4(C)[C@H]3[C@@H]2C JLISZLJGTVNTPC-UBWBUNFISA-N 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000007171 acid catalysis Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000002655 kraft paper Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- -1 yuccagenin ketone Chemical class 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Dioscorea containing dioscin is used as raw material, and is fermented by aspergillus niger to produce dioscin and citric acid. Through fermentation, the molecular structure of the diosgenin is kept unchanged, and the yield of the diosgenin is improved by times; after fermentation, the starch is converted into citric acid and enters a liquid phase, so that the materials put into the hydrolysis process are reduced, the consumption of acid, solvent and energy is reduced, and the production cost of each ton of diosgenin can be reduced by about 3 ten thousand yuan.
Description
The present invention is a kind of science fermentation process that is extracted diosgenin by yam.
Diosgenin is a precursor of producing multiple steroid drugs, the steroid hormone of produced worldwide, and diosgenin has drawn from more than 60%.Largest production state is a Mexico in the world, about 500 tons of annual production, and raw material variety is Dioscorea camposita and barbasco.Next is China, and raw material variety is Rhizome of Peltate Yam and Dioscorea nipponica Mak. Ningpo Yam Rhizome, and other all has a certain amount of production as states such as Guatemala, Puerto Rico, India.Estimate that it is 2400 tons that world's annual production in 1985 (not comprising China) will increase, rate of growth is 10% every year on average.
In early days, the method for producing diosgenin is a direct acid-hydrolysis method-Chinese yam sheet is put into hydrolytic decomposition pot, and with certain density sulfuric acid or hydrochloric acid catalysis hydrolysis, with organic solvent lixiviate diosgenin from hydrolyzate, sugar and starch is stayed in the waste residue and liquid then.This method hydrolysis is not thorough, and the saponin yield is low, generally reaches about 2%.
If through pre-fermentative processing, can make output improve 17～48%, yet existing pre-fermentative processing is with Chinese yam sheet water-sprinkling humidification, stacking in heaps, spontaneous fermentation, or be called from ferment and ferment.The soaking fermentation in the pond that has, the adding exogenous enzyme that has promotes fermentation.Simple and easy to do but the science not of aforesaid method, name is said from enzymic fermentation, assorted actually bacterium fermentation, any bacterial classification in the empty G﹠W all can be participated in, and often causes and goes mouldy.Wear Chinese yam rhizomes such as dragon, shield leaf, Foochow and hollyhock leaf, mouldy back saponin must be measured height, but the fusing point reduction can obtain table-yuccagenin and yuccagenin ketone through separating, without mouldy then not having.The appearance of these two kinds of derivatives makes quality product nonconforming, and output is descended.Particularly weather condition are to big from the enzymic fermentation influence, and fermentation condition and attenuation degree are difficult to control.Certain saponin factory is because of the heavy rain of a bust makes three, 40 tons of fresh ginger slices that fermenting all mould bad, loses to extract to be worth and to be forced to outwell.Not only be difficult to promote effectively hydrolysis to improve yield from enzymic fermentation, and, make the diosgenin structural modification, change other derivatives into, reduce the product fusing point, widen molten distance, cause bigger loss often owing to go mouldy in the part.Thereby the saponin yield of most producers is still 2～2.2%.
Be the approach of seeking to improve the saponin yield He utilize starch, Rothock, J.W. etc. did not once exceed direct acid-hydrolysis method level with terreus MF-118 bacterial strain enzymolysis D.barbasco diosgenin productive rate; The biological institute in Sichuan once carried out the research of pre-fermentation raising diosgenin productive rate and starch comprehensive utilization aspect, and propose that " Rhizome of Peltate Yam has kind can be influenced the enzyme that saponin is converted into sapogenin and exist; when the catalytic condition of suitable this kind of enzyme exists; its enzymic activity is strong, just can promote the raising of saponin productive rate in pre-fermenting process.Otherwise, after this kind of enzyme activity is damaged, though handle by pre-fermentation condition, all can not improve the saponin productive rate ", thereby, " without pre-fermentation, high temperature when zymamsis, high pressure steam material, have just played the effect of killing the saponin enzyme, so can not improve the saponin productive rate." that is the enzyme that produces during the fermentation of distiller's yeast, can not make saponin be degraded to secondary glycoside, thereby can not replace " from enzymic fermentation "; After 1 year, boiling at once after light industry institute in Shaanxi Province's pulverizes Rhizome of Peltate Yam, saccharification insert 1300
#Yeast carries out zymamsis, and per hundred jin of Chinese yams produce 96 degree 12.65 jin of alcohol (the rate of getting alcohol 27.3%), produce 0.78 jin of saponin (saponin content 0.9 to 1.5% in the raw material).
Can they only be conceived to utilize starch, improve the saponin yield to yeast fermentation and do not inquired into.The present invention is the disadvantage of radical cure " from enzymic fermentation ", several purebred black-koji moulds have been screened, carried out repeatedly fermenting experiment, cut-off connects total sapogenin that acid-hydrolysis method extracts then, after purebred black-koji mould fermentation, the total sapogenin that extracts through acid hydrolysis contrasts with the pre-saponin standard specimen of potato again, carry out tlc analysis, obtained two pairs of color spots.First couple of color spot R
f=0.45, with the R of standard specimen
fBe worth in full accordly, visible this color spot is exactly the color spot of diosgenin.Second pair color spot is little and look shallow, R
f=0.85, this is the color spot of other sapogenin or derivatives thereofs of coexistence, does not see other obvious color spots in addition.
To ferment and the total sapogenin sample of unfermentable two classes, gradation is successively used ether and benzene wash-out after the absorption of alumina dry post, obtain two elution peaks, collects product respectively, measures infrared spectra.As a result, the infrared spectra of the ether eluate of this two classes sample is identical ν＜`; Film; Max`〉cm
-1; 1057(C
3-OH and △
5), 986,926＜904, the 868(25R spiral shell stays alkane), contrasting decidable with Sadler's commodity spectrogram S-092 is diosgenin; Mass spectrum M/Z:414(M
+), the 139(base peak) 282,300, be diosgenin through library searching also decidable.The infrared spectra of the benzene eluate of this two classes sample is also identical, and they are same derivatives.
The above results shows, the change of black-koji mould fermentation not causing diosgenin molecular structure, and derivative is an inherent in the raw material, rather than produce in the fermentation.
Use without the general raw materials for production from enzymic fermentation, destroyed after the various enzymes that self exist through boiling, directly use the black-koji mould inoculation fermentation, extract diosgenin through acid hydrolysis again, its yield has improved more than 100% output all 4～5%.
In addition, it is raw material that the present invention adopts yam, through the black-koji mould one time fermentation, can produce two kinds of product-diosgenins and citric acid simultaneously on a cover production line.In the yam rhizome, except that containing various saponins and Mierocrystalline cellulose, also contain a large amount of starch, in enzymic fermentation, perhaps be decomposed into water and CO original
2Run away, perhaps mould bad, perhaps drain with spent acid solution, both enabled from acid solution, further to utilize starch, also neutralization in advance just can transfer other products to through Secondary Fermentation again.The present invention then is, under the black-koji mould effect, only needs one time fermentation, Yi Bian make the starch hydrolysis and change citric acid into, Yi Bian make sugar chain part enzymolysis in the diosgenin molecule, becomes secondary glycoside and sugar.(shown in the accompanying drawing schema) by fermentation can extract citric acid respectively from fermented liquid, extract diosgenin from filter residue.Through the check of Hubei bureau of import ﹠ export commodities inspection, the diosgenin of extraction and citric acid all reach export standard.
Recommendable implementation condition is as follows:
1. 5～15 gram tapioca flours are put into the 250ml conical flask, add 50ml water, wrap bottleneck, with 1.15～1.5kg/cm
2Open steam sterilization, and gelatinization 20～40 minutes is chilled to below 40 ℃, seals bottleneck after the inoculation, is placed on the shaking table 30～40 ℃ of temperature controls, ferment 3～4 days, takes out and filters, to the content and the purity of filtrate mensuration citric acid; To filter residue 4NH
2SO
4Ordinary-pressure hydrolysis 2～4 hours filters and is washed till nearly neutrality, and the baking universe changes in the Kai Shi extractor, with the sherwood oil (or 120 of 60～90 ℃ of boiling ranges
#Gasoline) lixiviate is 2～6 hours, concentrate then, and crystallization, drying is weighed and is surveyed fusing point, molten distance.
Also can adopt following processing condition.
2. at first the Chinese yam sheet is soaked, pump into behind the defibrination in the fermentor tank, make raw material: water=7～13: 50.Under agitation be warming up to 120～150 ℃ of sterilizations with steam, gelatinization 20～40 minutes is waited to be chilled to below 40 ℃ and can be inoculated, and 28～42 ℃ of temperature controls fermented 2～4 days.Stir speed (S.S.) is 120～180 rev/mins.Being close to zero with reducing sugar is fermentation termination.During fermentation ends, be warming up to 65～75 ℃ of boiling slips and squeeze into plate-and-frame filter press while hot and filter.Fermented liquid CaOO
3In and after-filtration, affination, the white powder crystal be citrate of lime, can get citric acid or other salts through processing again.Filter residue adds acid size mixing after, at 0.8～4kg/cm
2Vapour pressure under, hydrolysis 2～6 hours is squeezed into the plate-and-frame filter press press filtration then and is washed till nearly neutrality, drops into extractor through air stream drying to water content＜5% o'clock, with 120
#The gasoline lixiviate is to clean.Be concentrated into gasoline at concentration tank: saponin=30～60: changed over to and to weigh after crystallizer crystallization, filtration, the drying and survey fusing point, molten distance at 1 o'clock.
Because black-koji mould fermentation is without any negative reaction, and by fermentation with hydrolysis after twice filtration washing, in the hydrolyzate pigment and other impurity all seldom, so constant product quality, fusing point, molten be standard up to standard apart from primary crystallization.
Here, provide three embodiment.
Example 1 is got 1 part of tapioca flour, adds 5 parts of water, seals bottleneck with gauze and kraft paper in the 250ml conical flask of packing into, with 1.2～1.5kg/cm
2Steam sterilizing 20 minutes is chilled to below 40 ℃, from the inclined-plane that refrigerator is preserved, gets B
826Bacterial classification one ring is put into through adequately disinfected pure water, smashes with glass sphere and makes its suspension, gets suspension 1ml and injects conical flask by syringe, shaking table top fermentation 114 hours.Total reducing sugar be can get thus and sour rate 95.0%, citric acid purity 98.9%, diosgenin 4.48%, yield 88.4% changeed.
Example 2 is got 11 parts of tapioca flours, adds 50 parts of water, after the sterilization, inoculates B in conical flask
828Melanomyces spore suspension 1ml fermentation 110 hours.Total reducing sugar be can get thus and sour rate 85.9%, citric acid purity 91.1%, diosgenin yield 4.75%, yield 93.3% changeed.
Example 3 is got 11 parts of tapioca flours, adds 50 parts of water, adds a small amount of Ye Huamei, according to inoculating B with method sterilization back
828Black-koji mould suspension 1ml fermented 4 days.Total reducing sugar be can get and sour rate 90.7%, citric acid purity 71.1%, diosgenin yield 4.95%, yield 97.6% changeed.
Claims (3)
1, a kind of is the novel process of raw material coproduction diosgenin and citric acid with the yam that contains the pre-saponin of potato, it is characterized in that improving the saponin yield with the black-koji mould fermentation.
2, novel process as claimed in claim 1 is characterized in that remaining unchanged by the molecular structure of fermentation diosgenin.
3, novel process as claimed in claim 1 is characterized in that with a kind of raw material just can obtaining two products of diosgenin and citric acid simultaneously only through one time fermentation, also can appoint in the production and get one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN85108564A CN85108564B (en) | 1985-11-19 | 1985-11-19 | Fermentation of Aspergillus niger for coproduction of diosgenin and citric acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN85108564A CN85108564B (en) | 1985-11-19 | 1985-11-19 | Fermentation of Aspergillus niger for coproduction of diosgenin and citric acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN85108564A CN85108564A (en) | 1987-02-25 |
| CN85108564B true CN85108564B (en) | 1987-02-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN85108564A Expired CN85108564B (en) | 1985-11-19 | 1985-11-19 | Fermentation of Aspergillus niger for coproduction of diosgenin and citric acid |
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| Country | Link |
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| CN (1) | CN85108564B (en) |
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|---|---|---|---|---|
| CN101012474B (en) * | 2007-02-05 | 2010-09-15 | 大连理工大学 | A kind of method for preparing diosgenin by microbial transformation method |
| CN102154118B (en) * | 2010-12-31 | 2013-06-19 | 黄石兴华生化有限公司 | Method for producing citric acid by using AspergillusnigerHsy21 and by using fresh sweet potatoes as raw materials |
| CN117821260A (en) * | 2023-12-20 | 2024-04-05 | 西北农林科技大学 | A strain of Aspergillus niger and its application |
-
1985
- 1985-11-19 CN CN85108564A patent/CN85108564B/en not_active Expired
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| Publication number | Publication date |
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| CN85108564A (en) | 1987-02-25 |
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