CO6280552A2 - Plantas transgenicas modificadas para reducir el transporte de cadmio productos derivados y metodos relacionados - Google Patents

Plantas transgenicas modificadas para reducir el transporte de cadmio productos derivados y metodos relacionados

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Publication number
CO6280552A2
CO6280552A2 CO10085354A CO10085354A CO6280552A2 CO 6280552 A2 CO6280552 A2 CO 6280552A2 CO 10085354 A CO10085354 A CO 10085354A CO 10085354 A CO10085354 A CO 10085354A CO 6280552 A2 CO6280552 A2 CO 6280552A2
Authority
CO
Colombia
Prior art keywords
plants
modified
transgenic
transporters
nthma
Prior art date
Application number
CO10085354A
Other languages
English (en)
Inventor
Alec Hayes
Chengalrayan Kudithipudi
Der Hoeven Rutger Van
Original Assignee
Philip Morris Prod
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40481764&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CO6280552(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Philip Morris Prod filed Critical Philip Morris Prod
Publication of CO6280552A2 publication Critical patent/CO6280552A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La invención se dirige en parte a plantas transgénicas, que incluyen plantas de tabaco transgénicas y semillas derivadas, modificadas genéticamente para impedir el trasporte de Cadmio (Cd) del sistema de raíz a las porciones aéreas de plantas transgénicas al reducir los niveles de expresión de los transportadores relacionados con HMA. La invención incluye plantas de tabaco transgénicas modificadas genéticamente para expresar establemente una construcción de ARNi que codifica polinucleótidos de ARNi que posibilitan la degradación de variantes ARN NtHMA endógenas. La expresión reducida de transportadores NtHMA en plantas transgénicas resulta en contenido sustancialmente reducido de Cadmio (Cd) en la lámina de hoja. Varios productos consumibles que son sustancialmente libres o reducidos sustancialmente en el contenido de Cd se pueden producir al incorporar hojas derivadas de plantas de tabaco transgénicas modificadas para reducir la expresión de transportadores NtHMA.
CO10085354A 2007-12-13 2010-07-13 Plantas transgenicas modificadas para reducir el transporte de cadmio productos derivados y metodos relacionados CO6280552A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US99698207P 2007-12-13 2007-12-13

Publications (1)

Publication Number Publication Date
CO6280552A2 true CO6280552A2 (es) 2011-05-20

Family

ID=40481764

Family Applications (1)

Application Number Title Priority Date Filing Date
CO10085354A CO6280552A2 (es) 2007-12-13 2010-07-13 Plantas transgenicas modificadas para reducir el transporte de cadmio productos derivados y metodos relacionados

Country Status (11)

Country Link
US (4) US8383889B2 (es)
EP (3) EP2559766B1 (es)
CN (2) CN101952442A (es)
AP (1) AP3226A (es)
CO (1) CO6280552A2 (es)
DK (1) DK2231861T3 (es)
ES (2) ES2527272T3 (es)
PL (2) PL2231861T3 (es)
PT (1) PT2231861E (es)
SI (1) SI2231861T1 (es)
WO (1) WO2009074325A1 (es)

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EP2559766B1 (en) 2007-12-13 2017-10-18 Philip Morris Products S.A. Plants modified for reduced cadmium transport, derivative products, and related methods
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EP2565271A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Threonine synthase from Nicotiana tabacum and methods and uses thereof
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CN108338076B (zh) * 2012-02-08 2021-06-25 雷诺兹科技股份有限公司 具有可变量的环境污染物的烟草和用于制造这种品系的方法
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EP3480314A1 (en) 2017-11-03 2019-05-08 Philip Morris Products S.A. Regulation of alkaloid content
KR20200136921A (ko) 2018-03-28 2020-12-08 필립모리스 프로덕츠 에스.에이. 식물 내 아미노산 함량 조절
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JP7659547B2 (ja) 2019-10-01 2025-04-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節（ｉｎｖ）
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Also Published As

Publication number Publication date
US20140378667A1 (en) 2014-12-25
US10287598B2 (en) 2019-05-14
DK2231861T3 (en) 2015-01-19
PL2559766T3 (pl) 2018-02-28
WO2009074325A1 (en) 2009-06-18
EP2231861A1 (en) 2010-09-29
EP3327129A1 (en) 2018-05-30
US20130183738A1 (en) 2013-07-18
EP2231861B1 (en) 2014-10-29
PT2231861E (pt) 2015-02-03
US8383889B2 (en) 2013-02-26
CN104131030A (zh) 2014-11-05
AP3226A (en) 2015-04-30
EP2559766A1 (en) 2013-02-20
US8829161B2 (en) 2014-09-09
BRPI0820741A2 (pt) 2015-04-14
US9644206B2 (en) 2017-05-09
US20170218385A1 (en) 2017-08-03
SI2231861T1 (sl) 2015-02-27
US20090183280A1 (en) 2009-07-16
AP2010005311A0 (en) 2010-08-31
ES2645763T3 (es) 2017-12-07
EP2559766B1 (en) 2017-10-18
PL2231861T3 (pl) 2015-04-30
ES2527272T3 (es) 2015-01-22
CN101952442A (zh) 2011-01-19

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